                         SEQUENCE LISTING

<110>  The Governors of The University of Alberta
 
<120>  FUNCTIONALIZED BETA-SHEET STABILIZED MEMBRANE PROTEINS, 
       CONSTRUCTS COMPRISING SAME, AND METHODS OF FORMING AND USING SAME

<130>  M917 0005/TWB

<150>  US 62/041,587
<151>  2014-08-25

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  (octyl)Gly

<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  (octyl)Gly

<400>  1

Xaa Ser Leu Ser Leu Asp Xaa Asp 
1               5               


<210>  2
<211>  243
<212>  PRT
<213>  Rhodobacter sphaeroides

<400>  2

Met Thr Lys Lys Leu Leu Ala Glu Leu Leu Gly Thr Phe Ile Leu Val 
1               5                   10                  15      


Phe Phe Gly Cys Gly Ala Ala Val Leu Met Gly Pro Gln Ile Gly Met 
            20                  25                  30          


Leu Gly Ile Ser Leu Ala Phe Gly Leu Ser Ile Val Ala Ala Ala Tyr 
        35                  40                  45              


Ser Leu Gly Ala Ile Ser Gly Ala His Leu Asn Pro Ala Val Ser Leu 
    50                  55                  60                  


Gly Phe Leu Met Ala Gly Arg Met Pro Met Ala Glu Phe Gly Gly Tyr 
65                  70                  75                  80  


Val Leu Ala Gln Ile Ala Gly Ala Leu Leu Gly Ser Leu Val Val Phe 
                85                  90                  95      


Leu Ile Ala Ser Gly Lys Ala Gly Tyr Val Leu Ala Thr Asp Gly Leu 
            100                 105                 110         


Gly Gln Asn Gly Phe Gly Ala Gly Tyr Leu Gly Glu Tyr Ser Met Gly 
        115                 120                 125             


Ala Ala Leu Ile Phe Glu Leu Ile Ala Thr Phe Val Phe Val Ser Val 
    130                 135                 140                 


Ile Leu Ala Ala Thr Ala Ser His Val Ser Ser Ala Ser Thr Ala Leu 
145                 150                 155                 160 


Ala Gly Leu Ala Ile Gly Leu Thr Leu Thr Gly Ile His Leu Val Gly 
                165                 170                 175     


Ile Asn Val Thr Gly Val Ser Val Asn Pro Ala Arg Ser Leu Ala Pro 
            180                 185                 190         


Ala Leu Phe Val Gly Gly Lys Ala Leu Ser Asp Leu Trp Val Phe Ile 
        195                 200                 205             


Val Ala Pro Leu Ala Gly Gly Ala Ala Ala Gly Leu Ala His Ala Ser 
    210                 215                 220                 


Gly Phe Phe Arg Pro Gly Gly Ile Glu Pro Ala Pro Ala Thr Gly Ala 
225                 230                 235                 240 


Ala Thr Leu 
            


<210>  3
<211>  231
<212>  PRT
<213>  Escherichia coli

<400>  3

Met Phe Arg Lys Leu Ala Ala Glu Cys Phe Gly Thr Phe Cys Leu Val 
1               5                   10                  15      


Phe Gly Gly Cys Gly Ser Ala Val Leu Pro Ala Gly Phe Pro Glu Leu 
            20                  25                  30          


Gly Ile Gly Phe Ala Gly Val Ala Leu Ala Phe Gly Leu Thr Val Leu 
        35                  40                  45              


Thr Met Ala Phe Ala Val Gly His Ile Ser Gly Gly His Phe Asn Pro 
    50                  55                  60                  


Ala Val Thr Ile Gly Leu Trp Ala Gly Gly Arg Phe Pro Ala Lys Glu 
65                  70                  75                  80  


Val Val Gly Tyr Val Ile Ala Gln Val Val Gly Gly Ile Val Ala Ala 
                85                  90                  95      


Ala Leu Leu Tyr Leu Ile Ala Ser Gly Lys Thr Gly Phe Asp Ala Ala 
            100                 105                 110         


Ala Ser Gly Phe Ala Ser Asn Gly Tyr Gly Glu His Ser Pro Gly Gly 
        115                 120                 125             


Tyr Ser Met Leu Ser Ala Leu Val Val Glu Leu Val Leu Ser Ala Gly 
    130                 135                 140                 


Phe Leu Leu Val Ile His Gly Ala Thr Asp Lys Phe Ala Pro Ala Gly 
145                 150                 155                 160 


Phe Ala Pro Ile Ala Ile Gly Leu Ala Leu Thr Leu Ile His Leu Ile 
                165                 170                 175     


Ser Ile Pro Val Thr Asn Thr Ser Val Asn Pro Ala Arg Ser Thr Ala 
            180                 185                 190         


Val Ala Ile Phe Gln Gly Gly Trp Ala Leu Glu Gln Leu Trp Phe Phe 
        195                 200                 205             


Trp Val Val Pro Ile Val Gly Gly Ile Ile Gly Gly Leu Ile Tyr Arg 
    210                 215                 220                 


Thr Leu Leu Glu Lys Arg Asp 
225                 230     


<210>  4
<211>  281
<212>  PRT
<213>  Escherichia coli

<400>  4

Met Ser Gln Thr Ser Thr Leu Lys Gly Gln Cys Ile Ala Glu Phe Leu 
1               5                   10                  15      


Gly Thr Gly Leu Leu Ile Phe Phe Gly Val Gly Cys Val Ala Ala Leu 
            20                  25                  30          


Lys Val Ala Gly Ala Ser Phe Gly Gln Trp Glu Ile Ser Val Ile Trp 
        35                  40                  45              


Gly Leu Gly Val Ala Met Ala Ile Tyr Leu Thr Ala Gly Val Ser Gly 
    50                  55                  60                  


Ala His Leu Asn Pro Ala Val Thr Ile Ala Leu Trp Leu Phe Ala Cys 
65                  70                  75                  80  


Phe Asp Lys Arg Lys Val Ile Pro Phe Ile Val Ser Gln Val Ala Gly 
                85                  90                  95      


Ala Phe Cys Ala Ala Ala Leu Val Tyr Gly Leu Tyr Tyr Asn Leu Phe 
            100                 105                 110         


Phe Asp Phe Glu Gln Thr His His Ile Val Arg Gly Ser Val Glu Ser 
        115                 120                 125             


Val Asp Leu Ala Gly Thr Phe Ser Thr Tyr Pro Asn Pro His Ile Asn 
    130                 135                 140                 


Phe Val Gln Ala Phe Ala Val Glu Met Val Ile Thr Ala Ile Leu Met 
145                 150                 155                 160 


Gly Leu Ile Leu Ala Leu Thr Asp Asp Gly Asn Gly Val Pro Arg Gly 
                165                 170                 175     


Pro Leu Ala Pro Leu Leu Ile Gly Leu Leu Ile Ala Val Ile Gly Ala 
            180                 185                 190         


Ser Met Gly Pro Leu Thr Gly Phe Ala Met Asn Pro Ala Arg Asp Phe 
        195                 200                 205             


Gly Pro Lys Val Phe Ala Trp Leu Ala Gly Trp Gly Asn Val Ala Phe 
    210                 215                 220                 


Thr Gly Gly Arg Asp Ile Pro Tyr Phe Leu Val Pro Leu Phe Gly Pro 
225                 230                 235                 240 


Ile Val Gly Ala Ile Val Gly Ala Phe Ala Tyr Arg Lys Leu Ile Gly 
                245                 250                 255     


Arg His Leu Pro Cys Asp Ile Cys Val Val Glu Glu Lys Glu Thr Thr 
            260                 265                 270         


Thr Pro Ser Glu Gln Lys Ala Ser Leu 
        275                 280     


<210>  5
<211>  246
<212>  PRT
<213>  Methanobacter marburgensis

<400>  5

Met Val Ser Leu Thr Lys Arg Cys Ile Ala Glu Phe Ile Gly Thr Phe 
1               5                   10                  15      


Ile Leu Val Phe Phe Gly Ala Gly Ser Ala Ala Val Thr Leu Met Ile 
            20                  25                  30          


Ala Ser Gly Gly Thr Ser Pro Asn Pro Phe Asn Ile Gly Ile Gly Leu 
        35                  40                  45              


Leu Gly Gly Leu Gly Asp Trp Val Ala Ile Gly Leu Ala Phe Gly Phe 
    50                  55                  60                  


Ala Ile Ala Ala Ser Ile Tyr Ala Leu Gly Asn Ile Ser Gly Cys His 
65                  70                  75                  80  


Ile Asn Pro Ala Val Thr Ile Gly Leu Trp Ser Val Lys Lys Phe Pro 
                85                  90                  95      


Gly Arg Glu Val Val Pro Tyr Ile Ile Ala Gln Leu Leu Gly Ala Ala 
            100                 105                 110         


Phe Gly Ser Phe Ile Phe Leu Gln Cys Ala Gly Ile Gly Ala Ala Thr 
        115                 120                 125             


Val Gly Gly Leu Gly Ala Thr Ala Pro Phe Pro Gly Ile Ser Tyr Trp 
    130                 135                 140                 


Gln Ala Met Leu Ala Glu Val Val Gly Thr Phe Leu Leu Met Ile Thr 
145                 150                 155                 160 


Ile Met Gly Ile Ala Val Asp Glu Arg Ala Pro Lys Gly Phe Ala Gly 
                165                 170                 175     


Ile Ile Ile Gly Leu Thr Val Ala Gly Ile Ile Thr Thr Leu Gly Asn 
            180                 185                 190         


Ile Ser Gly Ser Ser Leu Asn Pro Ala Arg Thr Phe Gly Pro Tyr Leu 
        195                 200                 205             


Asn Asp Met Ile Phe Ala Gly Thr Asn Leu Trp Asn Tyr Tyr Pro Ile 
    210                 215                 220                 


Tyr Val Ile Gly Pro Ile Val Gly Ala Val Leu Ala Ala Leu Thr Tyr 
225                 230                 235                 240 


Gln Tyr Leu Thr Ser Glu 
                245     


<210>  6
<211>  269
<212>  PRT
<213>  Synechococcus elongatus

<400>  6

Met Lys Met Leu Arg Ala Leu Lys His His Trp Pro Glu Tyr Leu Ile 
1               5                   10                  15      


Glu Ala Trp Gly Leu Gly Leu Phe Met Val Ala Ala Gly Val Val Gly 
            20                  25                  30          


Thr Leu Val Phe Tyr Pro Gln Ser Pro Ala Tyr Gln Ala Ile Ala Asp 
        35                  40                  45              


Pro Phe Leu Gln Arg Val Val Met Gly Leu Gly Met Gly Leu Thr Ala 
    50                  55                  60                  


Met Ile Ile Met Tyr Ser Pro Trp Gly Lys Arg Ser Gly Ala His Ile 
65                  70                  75                  80  


Asn Pro Ala Val Thr Leu Thr Phe Tyr Arg Leu Lys Lys Ile Ala Ala 
                85                  90                  95      


Trp Asp Ala Phe Phe Tyr Val Val Phe Gln Phe Ile Gly Gly Leu Leu 
            100                 105                 110         


Gly Val Val Leu Val Ala Phe Leu Leu Gln Thr Pro Phe Thr Gln Ala 
        115                 120                 125             


Pro Val Asn Tyr Val Val Thr Val Pro Gly Lys Gln Gly Ala Ile Val 
    130                 135                 140                 


Ala Cys Ile Ala Glu Tyr Phe Ile Ala Val Leu Met Met Ser Met Val 
145                 150                 155                 160 


Leu Phe Thr Ser Asn Gln Pro Lys Leu Glu Arg Phe Thr Pro Phe Phe 
                165                 170                 175     


Ala Gly Cys Leu Ile Val Ser Tyr Val Ile Phe Glu Ser Pro Leu Ser 
            180                 185                 190         


Gly Phe Gly Met Asn Pro Ala Arg Thr Val Ala Ser Ala Leu Pro Ser 
        195                 200                 205             


Gly Ile Trp Thr Ala Ile Trp Leu Tyr Phe Leu Ala Pro Ile Ala Gly 
    210                 215                 220                 


Met Leu Thr Ala Ala Glu Leu Tyr Leu Arg Met Ile Gly Pro Arg Lys 
225                 230                 235                 240 


Ile Phe Cys Ala Lys Leu Tyr His Asp Pro Leu Tyr Arg Cys Ile His 
                245                 250                 255     


Cys Gly His Leu Ile His Trp His Arg Pro His Leu Arg 
            260                 265                 


<210>  7
<211>  279
<212>  PRT
<213>  Pichia pastoris

<400>  7

Met Pro Asp Ile Glu Asn Gln Ala Ala Asp Gly Gln Ala Glu Ile Lys 
1               5                   10                  15      


Pro Glu Asp Ala Pro Tyr Ile Thr Asn Ala Tyr Lys Pro Ala Tyr Ala 
            20                  25                  30          


Arg Trp Gly Phe Gly Ser Asp Ser Val Arg Asn His Phe Ile Ala Met 
        35                  40                  45              


Ser Gly Glu Phe Val Gly Thr Phe Leu Phe Leu Trp Ser Ala Phe Val 
    50                  55                  60                  


Ile Ala Gln Ile Ala Asn Gln Ala Pro Glu Thr Pro Asp Gly Gly Ser 
65                  70                  75                  80  


Asn Pro Ala Gln Leu Ile Met Ile Ser Phe Gly Phe Gly Phe Gly Val 
                85                  90                  95      


Met Val Gly Val Phe Ile Thr Tyr Arg Val Ser Gly Gly Asn Leu Asn 
            100                 105                 110         


Pro Ala Val Thr Leu Ala Leu Val Leu Ala Arg Ala Ile Pro Pro Phe 
        115                 120                 125             


Arg Gly Ile Leu Met Ala Phe Thr Gln Ile Val Ala Gly Met Ala Ala 
    130                 135                 140                 


Ala Gly Ala Ala Ser Ala Met Thr Pro Gly Glu Ile Ala Phe Ala Asn 
145                 150                 155                 160 


Ala Leu Gly Gly Gly Ala Ser Arg Thr Arg Gly Leu Phe Leu Glu Ala 
                165                 170                 175     


Phe Gly Thr Ala Ile Leu Cys Leu Thr Val Leu Met Leu Ala Val Glu 
            180                 185                 190         


Lys His Arg Ala Thr Trp Phe Ala Pro Phe Val Ile Gly Ile Ala Leu 
        195                 200                 205             


Leu Ile Ala His Leu Ile Cys Ile Tyr Tyr Thr Gly Ala Gly Leu Asn 
    210                 215                 220                 


Pro Ala Arg Ser Phe Gly Pro Ala Val Ala Ala Arg Ser Phe Pro Asn 
225                 230                 235                 240 


Tyr His Trp Ile Tyr Trp Leu Gly Pro Ile Leu Gly Ala Phe Leu Ala 
                245                 250                 255     


Tyr Ser Ile Trp Gln Met Trp Lys Trp Leu Asn Tyr Gln Thr Thr Asn 
            260                 265                 270         


Pro Gly Gln Asp Ser Asp Ala 
        275                 


<210>  8
<211>  281
<212>  PRT
<213>  Spinacia oleracea

<400>  8

Met Ser Lys Glu Val Ser Glu Glu Ala Gln Ala His Gln His Gly Lys 
1               5                   10                  15      


Asp Tyr Val Asp Pro Pro Pro Ala Pro Phe Phe Asp Leu Gly Glu Leu 
            20                  25                  30          


Lys Leu Trp Ser Phe Trp Arg Ala Ala Ile Ala Glu Phe Ile Ala Thr 
        35                  40                  45              


Leu Leu Phe Leu Tyr Ile Thr Val Ala Thr Val Ile Gly His Ser Lys 
    50                  55                  60                  


Glu Thr Val Val Cys Gly Ser Val Gly Leu Leu Gly Ile Ala Trp Ala 
65                  70                  75                  80  


Phe Gly Gly Met Ile Phe Val Leu Val Tyr Cys Thr Ala Gly Ile Ser 
                85                  90                  95      


Gly Gly His Ile Asn Pro Ala Val Thr Phe Gly Leu Phe Leu Ala Arg 
            100                 105                 110         


Lys Val Ser Leu Leu Arg Ala Leu Val Tyr Met Ile Ala Gln Cys Leu 
        115                 120                 125             


Gly Ala Ile Cys Gly Val Gly Leu Val Lys Ala Phe Met Lys Gly Pro 
    130                 135                 140                 


Tyr Asn Gln Phe Gly Gly Gly Ala Asn Ser Val Ala Leu Gly Tyr Asn 
145                 150                 155                 160 


Lys Gly Thr Ala Leu Gly Ala Glu Ile Ile Gly Thr Phe Val Leu Val 
                165                 170                 175     


Tyr Thr Val Phe Ser Ala Thr Asp Pro Lys Arg Ser Ala Arg Asp Ser 
            180                 185                 190         


His Val Pro Ile Leu Ala Pro Leu Pro Ile Gly Phe Ala Val Phe Met 
        195                 200                 205             


Val His Leu Ala Thr Ile Pro Ile Thr Gly Thr Gly Ile Asn Pro Ala 
    210                 215                 220                 


Arg Ser Phe Gly Ala Ala Val Ile Phe Asn Ser Asn Lys Val Trp Asp 
225                 230                 235                 240 


Asp Gln Trp Ile Phe Trp Val Gly Pro Phe Ile Gly Ala Ala Val Ala 
                245                 250                 255     


Ala Ala Tyr His Gln Tyr Val Leu Arg Ala Ala Ala Ile Lys Ala Leu 
            260                 265                 270         


Gly Ser Phe Arg Ser Asn Pro Thr Asn 
        275                 280     


<210>  9
<211>  269
<212>  PRT
<213>  Homo sapiens

<400>  9

Met Ala Ser Glu Phe Lys Lys Lys Leu Phe Trp Arg Ala Val Val Ala 
1               5                   10                  15      


Glu Phe Leu Ala Thr Thr Leu Phe Val Phe Ile Ser Ile Gly Ser Ala 
            20                  25                  30          


Leu Gly Phe Lys Tyr Pro Val Gly Asn Asn Gln Thr Ala Val Gln Asp 
        35                  40                  45              


Asn Val Lys Val Ser Leu Ala Phe Gly Leu Ser Ile Ala Thr Leu Ala 
    50                  55                  60                  


Gln Ser Val Gly His Ile Ser Gly Ala His Leu Asn Pro Ala Val Thr 
65                  70                  75                  80  


Leu Gly Leu Leu Leu Ser Cys Gln Ile Ser Ile Phe Arg Ala Leu Met 
                85                  90                  95      


Tyr Ile Ile Ala Gln Cys Val Gly Ala Ile Val Ala Thr Ala Ile Leu 
            100                 105                 110         


Ser Gly Ile Thr Ser Ser Leu Thr Gly Asn Ser Leu Gly Arg Asn Asp 
        115                 120                 125             


Leu Ala Asp Gly Val Asn Ser Gly Gln Gly Leu Gly Ile Glu Ile Ile 
    130                 135                 140                 


Gly Thr Leu Gln Leu Val Leu Cys Val Leu Ala Thr Thr Asp Arg Arg 
145                 150                 155                 160 


Arg Arg Asp Leu Gly Gly Ser Ala Pro Leu Ala Ile Gly Leu Ser Val 
                165                 170                 175     


Ala Leu Gly His Leu Leu Ala Ile Asp Tyr Thr Gly Cys Gly Ile Asn 
            180                 185                 190         


Pro Ala Arg Ser Phe Gly Ser Ala Val Ile Thr His Asn Phe Ser Asn 
        195                 200                 205             


His Trp Ile Phe Trp Val Gly Pro Phe Ile Gly Gly Ala Leu Ala Val 
    210                 215                 220                 


Leu Ile Tyr Asp Phe Ile Leu Ala Pro Arg Ser Ser Asp Leu Thr Asp 
225                 230                 235                 240 


Arg Val Lys Val Trp Thr Ser Gly Gln Val Glu Glu Tyr Asp Leu Asp 
                245                 250                 255     


Ala Asp Asp Ile Asn Ser Arg Val Glu Met Lys Pro Lys 
            260                 265                 


<210>  10
<211>  323
<212>  PRT
<213>  Homo sapiens

<400>  10

Met Ser Asp Arg Pro Thr Ala Arg Arg Trp Gly Lys Cys Gly Pro Leu 
1               5                   10                  15      


Cys Thr Arg Glu Asn Ile Met Val Ala Phe Lys Gly Val Trp Thr Gln 
            20                  25                  30          


Ala Phe Trp Lys Ala Val Thr Ala Glu Phe Leu Ala Met Leu Ile Phe 
        35                  40                  45              


Val Leu Leu Ser Leu Gly Ser Thr Ile Asn Trp Gly Gly Thr Glu Lys 
    50                  55                  60                  


Pro Leu Pro Val Asp Met Val Leu Ile Ser Leu Cys Phe Gly Leu Ser 
65                  70                  75                  80  


Ile Ala Thr Met Val Gln Cys Phe Gly His Ile Ser Gly Gly His Ile 
                85                  90                  95      


Asn Pro Ala Val Thr Val Ala Met Val Cys Thr Arg Lys Ile Ser Ile 
            100                 105                 110         


Ala Lys Ser Val Phe Tyr Ile Ala Ala Gln Cys Leu Gly Ala Ile Ile 
        115                 120                 125             


Gly Ala Gly Ile Leu Tyr Leu Val Thr Pro Pro Ser Val Val Gly Gly 
    130                 135                 140                 


Leu Gly Val Thr Met Val His Gly Asn Leu Thr Ala Gly His Gly Leu 
145                 150                 155                 160 


Leu Val Glu Leu Ile Ile Thr Phe Gln Leu Val Phe Thr Ile Phe Ala 
                165                 170                 175     


Ser Cys Asp Ser Lys Arg Thr Asp Val Thr Gly Ser Ile Ala Leu Ala 
            180                 185                 190         


Ile Gly Phe Ser Val Ala Ile Gly His Leu Phe Ala Ile Asn Tyr Thr 
        195                 200                 205             


Gly Ala Ser Met Asn Pro Ala Arg Ser Phe Gly Pro Ala Val Ile Met 
    210                 215                 220                 


Gly Asn Trp Glu Asn His Trp Ile Tyr Trp Val Gly Pro Ile Ile Gly 
225                 230                 235                 240 


Ala Val Leu Ala Gly Gly Leu Tyr Glu Tyr Val Phe Cys Pro Asp Val 
                245                 250                 255     


Glu Phe Lys Arg Arg Phe Lys Glu Ala Phe Ser Lys Ala Ala Gln Gln 
            260                 265                 270         


Thr Lys Gly Ser Tyr Met Glu Val Glu Asp Asn Arg Ser Gln Val Glu 
        275                 280                 285             


Thr Asp Asp Leu Ile Leu Lys Pro Gly Val Val His Val Ile Asp Val 
    290                 295                 300                 


Asp Arg Gly Glu Glu Lys Lys Gly Lys Asp Gln Ser Gly Glu Val Leu 
305                 310                 315                 320 


Ser Ser Val 
            


<210>  11
<211>  295
<212>  PRT
<213>  Homo sapiens

<400>  11

Met Gln Pro Glu Gly Ala Glu Lys Gly Lys Ser Phe Lys Gln Arg Leu 
1               5                   10                  15      


Val Leu Lys Ser Ser Leu Ala Lys Glu Thr Leu Ser Glu Phe Leu Gly 
            20                  25                  30          


Thr Phe Ile Leu Ile Val Leu Gly Cys Gly Cys Val Ala Gln Ala Ile 
        35                  40                  45              


Leu Ser Arg Gly Arg Phe Gly Gly Val Ile Thr Ile Asn Val Gly Phe 
    50                  55                  60                  


Ser Met Ala Val Ala Met Ala Ile Tyr Val Ala Gly Gly Val Ser Gly 
65                  70                  75                  80  


Gly His Ile Asn Pro Ala Val Ser Leu Ala Met Cys Leu Phe Gly Arg 
                85                  90                  95      


Met Lys Trp Phe Lys Leu Pro Phe Tyr Val Gly Ala Gln Phe Leu Gly 
            100                 105                 110         


Ala Phe Val Gly Ala Ala Thr Val Phe Gly Ile Tyr Tyr Asp Gly Leu 
        115                 120                 125             


Met Ser Phe Ala Gly Gly Lys Leu Leu Ile Val Gly Glu Asn Ala Thr 
    130                 135                 140                 


Ala His Ile Phe Ala Thr Tyr Pro Ala Pro Tyr Leu Ser Leu Ala Asn 
145                 150                 155                 160 


Ala Phe Ala Asp Gln Val Val Ala Thr Met Ile Leu Leu Ile Ile Val 
                165                 170                 175     


Phe Ala Ile Phe Asp Ser Arg Asn Leu Gly Ala Pro Arg Gly Leu Glu 
            180                 185                 190         


Pro Ile Ala Ile Gly Leu Leu Ile Ile Val Ile Ala Ser Ser Leu Gly 
        195                 200                 205             


Leu Asn Ser Gly Cys Ala Met Asn Pro Ala Arg Asp Leu Ser Pro Arg 
    210                 215                 220                 


Leu Phe Thr Ala Leu Ala Gly Trp Gly Phe Glu Val Phe Arg Ala Gly 
225                 230                 235                 240 


Asn Asn Phe Trp Trp Ile Pro Val Val Gly Pro Leu Val Gly Ala Val 
                245                 250                 255     


Ile Gly Gly Leu Ile Tyr Val Leu Val Ile Glu Ile His His Pro Glu 
            260                 265                 270         


Pro Asp Ser Val Phe Lys Thr Glu Gln Ser Glu Asp Lys Pro Glu Lys 
        275                 280                 285             


Tyr Glu Leu Ser Val Ile Met 
    290                 295 


<210>  12
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  (octyl)Gly

<220>
<221>  MOD_RES
<222>  (7)..(7)
<223>  (octyl)Gly

<220>
<221>  MOD_RES
<222>  (9)..(9)
<223>  (propargyl)Gly

<400>  12

Xaa Ser Leu Ser Leu Asp Xaa Asp Xaa 
1               5                   


<210>  13
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  azidohomoalanine

<220>
<221>  MOD_RES
<222>  (2)..(2)
<223>  (octyl)Gly

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  (octyl)Gly

<400>  13

Xaa Xaa Ser Leu Ser Leu Asp Xaa Asp 
1               5                   


<210>  14
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  (propargyl)Gly

<220>
<221>  MOD_RES
<222>  (2)..(2)
<223>  (octyl)Gly

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  (octyl)Gly

<400>  14

Xaa Xaa Ser Leu Ser Leu Asp Xaa Asp 
1               5                   


<210>  15
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  (octyl)Gly

<220>
<221>  MOD_RES
<222>  (5)..(5)
<223>  amino hexenoic acid

<220>
<221>  MOD_RES
<222>  (8)..(8)
<223>  (octyl)Gly

<400>  15

Xaa Ser Leu Ser Xaa Leu Asp Xaa Asp Gly Leu 
1               5                   10      


<210>  16
<211>  6003
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid used for expression of AqpZ in Eschericia coli.

<400>  16
atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa       60

ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt      120

tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttta      180

atcacgcttt tccagcaggg tccggtaaat cagaccaccg ataatgccgc cgacaattgg      240

caccacccag aagaaccaca gttgttctaa tgcccagccg ccctggaaga tagcaaccgc      300

ggtgctgcgc gccgggttaa cagaagtgtt agtcaccgga atactaatta agtgaatcag      360

ggttaaggcc agaccaatag cgatcggcgc aaaacctgcc ggcgcgaatt tgtcggttgc      420

gccgtggatc accaacagga aacctgcact caataccagt tcaactacca gcgcggaaag      480

catggaataa ccgcctggtg aatgctcgcc ataaccgtta gaagcaaaac cgctggctgc      540

cgcgtcaaaa cccgttttac cactggcaat taaatacagc agcgccgctg caacaatacc      600

gccgacaacc tgggcaatta cgtagccaac gacttctttt gccggaaaac gtccgccagc      660

ccataaacca atagtgaccg ccgggttaaa atgaccacca gaaatatgac caacagcaaa      720

ggccatcgtc agaacggtca gaccgaacgc caacgccacg ccggcaaaac caatgcctaa      780

ttccgggaag cctgcggcca gtacagcact accacagcca ccaaaaacaa gccagaaagt      840

accaaaacat tcagctgcta attttctgaa catatggctg ccgcgcggca ccaggccgct      900

gctgtgatga tgatgatgat ggctgctgcc catggtatat ctccttctta aagttaaaca      960

aaattatttc tagaggggaa ttgttatccg ctcacaattc ccctatagtg agtcgtatta     1020

atttcgcggg atcgagatct cgatcctcta cgccggacgc atcgtggccg gcatcaccgg     1080

cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc     1140

tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc     1200

cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa     1260

cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg     1320

agatcccgga caccatcgaa tggcgcaaaa cctttcgcgg tatggcatga tagcgcccgg     1380

aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt     1440

atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg     1500

cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg     1560

tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg     1620

ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg     1680

ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc     1740

acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg     1800

atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg     1860

accagacacc catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg     1920

agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg     1980

tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc     2040

cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa     2100

tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg     2160

gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg     2220

gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg     2280

attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg     2340

cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc     2400

ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac     2460

aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtaag ttagctcact     2520

cattaggcac cgggatctcg accgatgccc ttgagagcct tcaacccagt cagctccttc     2580

cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa     2640

ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg     2700

agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa     2760

gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc     2820

atggcggccc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg     2880

cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac     2940

tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt     3000

tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc     3060

gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat     3120

tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca     3180

caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct     3240

cgtttcatcg gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg     3300

accaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg     3360

cttctggaga aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt     3420

cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa     3480

aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg     3540

agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg     3600

acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga     3660

ttgtactgag agtgcaccat atatgcggtg tgaaataccg cacagatgcg taaggagaaa     3720

ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg     3780

gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg     3840

ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa     3900

ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg     3960

acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc     4020

tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc     4080

ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc     4140

ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg     4200

ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc     4260

actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga     4320

gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc     4380

tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac     4440

caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg     4500

atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc     4560

acgttaaggg attttggtca tgaacaataa aactgtctgc ttacataaac agtaatacaa     4620

ggggtgttat gagccatatt caacgggaaa cgtcttgctc taggccgcga ttaaattcca     4680

acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg     4740

cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca     4800

aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat     4860

ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca     4920

ccactgcgat ccccgggaaa acagcattcc aggtattaga agaatatcct gattcaggtg     4980

aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta     5040

attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata     5100

acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag     5160

tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg     5220

atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg     5280

gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg     5340

agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata     5400

tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaagaatta attcatgagc     5460

ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc     5520

cgaaaagtgc cacctgaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt     5580

tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca     5640

aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta     5700

aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta     5760

cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg     5820

aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga     5880

aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg     5940

ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg     6000

cca                                                                   6003


