                         SEQUENCE LISTING

<110>  The Coca-Cola Company
 
<120>  METHODS FOR PREPARING REBAUDIOSIDE I AND USES

<130>  12600.105145

<140>  PCT/US2015/045906
<141>  2015-08-19

<150>  62/039,344
<151>  2014-08-19

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  1397
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized expression sequence

<400>  1
ccatggccca tatggaaaac aaaaccgaaa ccaccgttcg tcgtcgtcgc cgtattattc       60

tgtttccggt tccgtttcag ggtcatatta atccgattct gcagctggca aatgtgctgt      120

atagcaaagg ttttagcatt accatttttc ataccaattt taacaaaccg aaaaccagca      180

attatccgca ttttaccttt cgctttattc tggataatga tccgcaggat gaacgcatta      240

gcaatctgcc gacacatggt ccgctggcag gtatgcgtat tccgattatt aacgaacatg      300

gtgcagatga actgcgtcgt gaactggaac tgctgatgct ggcaagcgaa gaagatgaag      360

aagttagctg tctgattacc gatgcactgt ggtattttgc acagagcgtt gcagatagcc      420

tgaatctgcg tcgtctggtt ctgatgacca gcagcctgtt taactttcat gcacatgtta      480

gcctgccgca gtttgatgaa ctgggttatc tggatccgga tgataaaacc cgtctggaag      540

aacaggcaag cggttttccg atgctgaaag tgaaagatat caaaagcgcc tatagcaatt      600

ggcagattct gaaagaaatt ctgggcaaaa tgattaaaca gaccaaagca agcagcggtg      660

ttatttggaa tagctttaaa gaactggaag aaagcgaact ggaaaccgtg attcgtgaaa      720

ttccggcacc gagctttctg attccgctgc cgaaacatct gaccgcaagc agcagcagcc      780

tgctggatca tgatcgtacc gtttttcagt ggctggatca gcagcctccg agcagcgttc      840

tgtatgttag ctttggtagc accagcgaag ttgatgaaaa agattttctg gaaattgccc      900

gtggtctggt tgatagcaaa cagagctttc tgtgggttgt tcgtccgggt tttgttaaag      960

gtagcacctg ggttgaaccg ctgccggatg gttttctggg tgaacgtggt cgtattgtta     1020

aatgggttcc gcagcaagaa gttctggcac acggcgcaat tggtgcattt tggacccata     1080

gcggttggaa tagcaccctg gaaagcgttt gtgaaggtgt tccgatgatt tttagcgatt     1140

ttggtctgga tcagccgctg aatgcacgtt atatgagtga tgttctgaaa gtgggtgtgt     1200

atctggaaaa tggttgggaa cgtggtgaaa ttgcaaatgc aattcgtcgt gttatggtgg     1260

atgaagaagg tgaatatatt cgtcagaatg cccgtgttct gaaacagaaa gcagatgtta     1320

gcctgatgaa aggtggtagc agctatgaaa gcctggaaag tctggttagc tatattagca     1380

gcctgtaata actcgag                                                    1397


<210>  2
<211>  1442
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon-optimized expression sequence

<400>  2
ccatggcaca tatggcaacc agcgatagca ttgttgatga tcgtaaacag ctgcatgttg       60

caacctttcc gtggctggca tttggtcata ttctgccgta tctgcagctg agcaaactga      120

ttgcagaaaa aggtcataaa gtgagctttc tgagcaccac ccgtaatatt cagcgtctga      180

gcagccatat tagtccgctg attaatgttg ttcagctgac cctgcctcgt gttcaagaac      240

tgccggaaga tgccgaagca accaccgatg ttcatccgga agatattccg tatctgaaaa      300

aagcaagtga tggtctgcag ccggaagtta cccgttttct ggaacagcat agtccggatt      360

ggatcatcta tgattatacc cattattggc tgccgagcat tgcagcaagc ctgggtatta      420

gccgtgcaca ttttagcgtt accaccccgt gggcaattgc atatatgggt ccgagcgcag      480

atgcaatgat taatggtagt gatggtcgta ccaccgttga agatctgacc acccctccga      540

aatggtttcc gtttccgacc aaagtttgtt ggcgtaaaca tgatctggca cgtctggttc      600

cgtataaagc accgggtatt agtgatggtt atcgtatggg tctggttctg aaaggtagcg      660

attgtctgct gagcaaatgc tatcatgaat ttggcaccca gtggctgccg ctgctggaaa      720

ccctgcatca ggttccggtt gttccggtgg gtctgctgcc tccggaagtt ccgggtgatg      780

aaaaagatga aacctgggtt agcatcaaaa aatggctgga tggtaaacag aaaggtagcg      840

tggtttatgt tgcactgggt agcgaagttc tggttagcca gaccgaagtt gttgaactgg      900

cactgggtct ggaactgagc ggtctgccgt ttgtttgggc atatcgtaaa ccgaaaggtc      960

cggcaaaaag cgatagcgtt gaactgccgg atggttttgt tgaacgtacc cgtgatcgtg     1020

gtctggtttg gaccagctgg gcacctcagc tgcgtattct gagccatgaa agcgtttgtg     1080

gttttctgac ccattgtggt agcggtagca ttgtggaagg tctgatgttt ggtcatccgc     1140

tgattatgct gccgattttt ggtgatcagc cgctgaatgc acgtctgctg gaagataaac     1200

aggttggtat tgaaattccg cgtaatgaag aagatggttg cctgaccaaa gaaagcgttg     1260

cacgtagcct gcgtagcgtt gttgttgaaa aagaaggcga aatctataaa gccaatgcac     1320

gtgaactgag caaaatctat aatgatacca aagtggaaaa agaatatgtg agccagttcg     1380

tggattatct ggaaaaaaac acccgtgcag ttgccattga tcacgaaagc taatgactcg     1440

ag                                                                    1442


<210>  3
<211>  442
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Expressed artificial UGTSL2

<400>  3

Met Ala Thr Asn Leu Arg Val Leu Met Phe Pro Trp Leu Ala Tyr Gly 
1               5                   10                  15      


His Ile Ser Pro Phe Leu Asn Ile Ala Lys Gln Leu Ala Asp Arg Gly 
            20                  25                  30          


Phe Leu Ile Tyr Leu Cys Ser Thr Arg Ile Asn Leu Glu Ser Ile Ile 
        35                  40                  45              


Lys Lys Ile Pro Glu Lys Tyr Ala Asp Ser Ile His Leu Ile Glu Leu 
    50                  55                  60                  


Gln Leu Pro Glu Leu Pro Glu Leu Pro Pro His Tyr His Thr Thr Asn 
65                  70                  75                  80  


Gly Leu Pro Pro His Leu Asn Pro Thr Leu His Lys Ala Leu Lys Met 
                85                  90                  95      


Ser Lys Pro Asn Phe Ser Arg Ile Leu Gln Asn Leu Lys Pro Asp Leu 
            100                 105                 110         


Leu Ile Tyr Asp Val Leu Gln Pro Trp Ala Glu His Val Ala Asn Glu 
        115                 120                 125             


Gln Asn Ile Pro Ala Gly Lys Leu Leu Thr Ser Cys Ala Ala Val Phe 
    130                 135                 140                 


Ser Tyr Phe Phe Ser Phe Arg Lys Asn Pro Gly Val Glu Phe Pro Phe 
145                 150                 155                 160 


Pro Ala Ile His Leu Pro Glu Val Glu Lys Val Lys Ile Arg Glu Ile 
                165                 170                 175     


Leu Ala Lys Glu Pro Glu Glu Gly Gly Arg Leu Asp Glu Gly Asn Lys 
            180                 185                 190         


Gln Met Met Leu Met Cys Thr Ser Arg Thr Ile Glu Ala Lys Tyr Ile 
        195                 200                 205             


Asp Tyr Cys Thr Glu Leu Cys Asn Trp Lys Val Val Pro Val Gly Pro 
    210                 215                 220                 


Pro Phe Gln Asp Leu Ile Thr Asn Asp Ala Asp Asn Lys Glu Leu Ile 
225                 230                 235                 240 


Asp Trp Leu Gly Thr Lys His Glu Asn Ser Thr Val Phe Val Ser Phe 
                245                 250                 255     


Gly Ser Glu Tyr Phe Leu Ser Lys Glu Asp Met Glu Glu Val Ala Phe 
            260                 265                 270         


Ala Leu Glu Leu Ser Asn Val Asn Phe Ile Trp Val Ala Arg Phe Pro 
        275                 280                 285             


Lys Gly Glu Glu Arg Asn Leu Glu Asp Ala Leu Pro Lys Gly Phe Leu 
    290                 295                 300                 


Glu Arg Ile Gly Glu Arg Gly Arg Val Leu Asp Lys Phe Ala Pro Gln 
305                 310                 315                 320 


Pro Arg Ile Leu Asn His Pro Ser Thr Gly Gly Phe Ile Ser His Cys 
                325                 330                 335     


Gly Trp Asn Ser Ala Met Glu Ser Ile Asp Phe Gly Val Pro Ile Ile 
            340                 345                 350         


Ala Met Pro Ile His Asn Asp Gln Pro Ile Asn Ala Lys Leu Met Val 
        355                 360                 365             


Glu Leu Gly Val Ala Val Glu Ile Val Arg Asp Asp Asp Gly Lys Ile 
    370                 375                 380                 


His Arg Gly Glu Ile Ala Glu Thr Leu Lys Ser Val Val Thr Gly Glu 
385                 390                 395                 400 


Thr Gly Glu Ile Leu Arg Ala Lys Val Arg Glu Ile Ser Lys Asn Leu 
                405                 410                 415     


Lys Ser Ile Arg Asp Glu Glu Met Asp Ala Val Ala Glu Glu Leu Ile 
            420                 425                 430         


Gln Leu Cys Arg Asn Ser Asn Lys Ser Lys 
        435                 440         


<210>  4
<211>  458
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Expressed artificial UGT76G1

<400>  4

Met Glu Asn Lys Thr Glu Thr Thr Val Arg Arg Arg Arg Arg Ile Ile 
1               5                   10                  15      


Leu Phe Pro Val Pro Phe Gln Gly His Ile Asn Pro Ile Leu Gln Leu 
            20                  25                  30          


Ala Asn Val Leu Tyr Ser Lys Gly Phe Ser Ile Thr Ile Phe His Thr 
        35                  40                  45              


Asn Phe Asn Lys Pro Lys Thr Ser Asn Tyr Pro His Phe Thr Phe Arg 
    50                  55                  60                  


Phe Ile Leu Asp Asn Asp Pro Gln Asp Glu Arg Ile Ser Asn Leu Pro 
65                  70                  75                  80  


Thr His Gly Pro Leu Ala Gly Met Arg Ile Pro Ile Ile Asn Glu His 
                85                  90                  95      


Gly Ala Asp Glu Leu Arg Arg Glu Leu Glu Leu Leu Met Leu Ala Ser 
            100                 105                 110         


Glu Glu Asp Glu Glu Val Ser Cys Leu Ile Thr Asp Ala Leu Trp Tyr 
        115                 120                 125             


Phe Ala Gln Ser Val Ala Asp Ser Leu Asn Leu Arg Arg Leu Val Leu 
    130                 135                 140                 


Met Thr Ser Ser Leu Phe Asn Phe His Ala His Val Ser Leu Pro Gln 
145                 150                 155                 160 


Phe Asp Glu Leu Gly Tyr Leu Asp Pro Asp Asp Lys Thr Arg Leu Glu 
                165                 170                 175     


Glu Gln Ala Ser Gly Phe Pro Met Leu Lys Val Lys Asp Ile Lys Ser 
            180                 185                 190         


Ala Tyr Ser Asn Trp Gln Ile Leu Lys Glu Ile Leu Gly Lys Met Ile 
        195                 200                 205             


Lys Gln Thr Lys Ala Ser Ser Gly Val Ile Trp Asn Ser Phe Lys Glu 
    210                 215                 220                 


Leu Glu Glu Ser Glu Leu Glu Thr Val Ile Arg Glu Ile Pro Ala Pro 
225                 230                 235                 240 


Ser Phe Leu Ile Pro Leu Pro Lys His Leu Thr Ala Ser Ser Ser Ser 
                245                 250                 255     


Leu Leu Asp His Asp Arg Thr Val Phe Gln Trp Leu Asp Gln Gln Pro 
            260                 265                 270         


Pro Ser Ser Val Leu Tyr Val Ser Phe Gly Ser Thr Ser Glu Val Asp 
        275                 280                 285             


Glu Lys Asp Phe Leu Glu Ile Ala Arg Gly Leu Val Asp Ser Lys Gln 
    290                 295                 300                 


Ser Phe Leu Trp Val Val Arg Pro Gly Phe Val Lys Gly Ser Thr Trp 
305                 310                 315                 320 


Val Glu Pro Leu Pro Asp Gly Phe Leu Gly Glu Arg Gly Arg Ile Val 
                325                 330                 335     


Lys Trp Val Pro Gln Gln Glu Val Leu Ala His Gly Ala Ile Gly Ala 
            340                 345                 350         


Phe Trp Thr His Ser Gly Trp Asn Ser Thr Leu Glu Ser Val Cys Glu 
        355                 360                 365             


Gly Val Pro Met Ile Phe Ser Asp Phe Gly Leu Asp Gln Pro Leu Asn 
    370                 375                 380                 


Ala Arg Tyr Met Ser Asp Val Leu Lys Val Gly Val Tyr Leu Glu Asn 
385                 390                 395                 400 


Gly Trp Glu Arg Gly Glu Ile Ala Asn Ala Ile Arg Arg Val Met Val 
                405                 410                 415     


Asp Glu Glu Gly Glu Tyr Ile Arg Gln Asn Ala Arg Val Leu Lys Gln 
            420                 425                 430         


Lys Ala Asp Val Ser Leu Met Lys Gly Gly Ser Ser Tyr Glu Ser Leu 
        435                 440                 445             


Glu Ser Leu Val Ser Tyr Ile Ser Ser Leu 
    450                 455             


