SEQUENCE LISTING

<110>  SuppreMol GmbH
 
<120>  Novel antibodies directed to Fc gamma receptor IIB and Fc epsilon receptor

<130>  SUP15095PCT

<150>  EP14002825.9
<151>  2014-08-13


<160>  38    

<170>  PatentIn version 3.5

<210>  1
<211>  113
<212>  PRT
<213>  Rattus norvegicus


<220>
<221>  MISC_FEATURE
<222>  (1)..(113)
<223>  VH r8A6

<400>  1

Glu Val Gln Leu Val Glu Ser Gly Gly Asp Leu Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Tyr 
            20                  25                  30          


Tyr Met Ala Trp Val Arg Gln Ala Pro Lys Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Ser Ile Ser Ser Asp Gly Ser Asn Thr Tyr Tyr Gly Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Thr Arg Ser Asn Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ser Glu Asp Thr Ala Thr Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Gly Asp Tyr Trp Gly Gln Gly Val Met Val Thr Val Ser 
            100                 105                 110         


Ser 
    


<210>  2
<211>  108
<212>  PRT
<213>  Rattus norvegicus


<220>
<221>  MISC_FEATURE
<223>  VL r8A6

<220>
<221>  MISC_FEATURE
<222>  (1)..(108)
<223>  VL r8A6

<400>  2

Asn Ile Val Met Thr Gln Ser Pro Thr Ser Met Phe Ile Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Met Asn Cys Lys Ala Ser Gln Asn Val Gly Thr Tyr 
            20                  25                  30          


Val Asp Trp Phe Gln Gln Lys Thr Gly Gln Ser Pro Thr Leu Leu Ile 
        35                  40                  45              


Phe Gly Ala Ser Asn Arg Tyr Thr Gly Val Pro Asp Arg Phe Thr Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Ser Ile Ser Asn Met Gln Ala 
65                  70                  75                  80  


Glu Asp Leu Ala Val Tyr Tyr Cys Leu Gln Tyr Asn Tyr His Pro Tyr 
                85                  90                  95      


Thr Phe Gly Pro Gly Thr Thr Leu Glu Leu Lys Arg 
            100                 105             


<210>  3
<211>  113
<212>  PRT
<213>  Artificial

<220>
<223>  humanized antibody


<220>
<221>  MISC_FEATURE
<222>  (1)..(113)
<223>  VH hu8A6

<400>  3

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Tyr 
            20                  25                  30          


Tyr Met Ala Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Ser Ile Ser Tyr Asp Gly Ser Asn Lys Tyr Tyr Gly Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Pro Gly Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
            100                 105                 110         


Ser 
    


<210>  4
<211>  108
<212>  PRT
<213>  Artificial

<220>
<223>  humanized antibody


<220>
<221>  MISC_FEATURE
<222>  (1)..(108)
<223>  VL hu8A6

<400>  4

Gln Ile Val Met Thr Gln Ser Pro Ala Thr Leu Ser Val Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Val Gly Ser Tyr 
            20                  25                  30          


Val Asp Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 
        35                  40                  45              


Tyr Gly Ala Ser Thr Arg Tyr Thr Gly Ile Pro Ala Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Ser 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Leu Gln Tyr Asn Asn His Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg 
            100                 105             


<210>  5
<211>  181
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(181)
<223>  human FcyRIIB

<400>  5

Met Gly Thr Pro Ala Ala Pro Pro Lys Ala Val Leu Lys Leu Glu Pro 
1               5                   10                  15      


Gln Trp Ile Asn Val Leu Gln Glu Asp Ser Val Thr Leu Thr Cys Arg 
            20                  25                  30          


Gly Thr His Ser Pro Glu Ser Asp Ser Ile Gln Trp Phe His Asn Gly 
        35                  40                  45              


Asn Leu Ile Pro Thr His Thr Gln Pro Ser Tyr Arg Phe Lys Ala Asn 
    50                  55                  60                  


Asn Asn Asp Ser Gly Glu Tyr Thr Cys Gln Thr Gly Gln Thr Ser Leu 
65                  70                  75                  80  


Ser Asp Pro Val His Leu Thr Val Leu Ser Glu Trp Leu Val Leu Gln 
                85                  90                  95      


Thr Pro His Leu Glu Phe Gln Glu Gly Glu Thr Ile Val Leu Arg Cys 
            100                 105                 110         


His Ser Trp Lys Asp Lys Pro Leu Val Lys Val Thr Phe Phe Gln Asn 
        115                 120                 125             


Gly Lys Ser Lys Lys Phe Ser Arg Ser Asp Pro Asn Phe Ser Ile Pro 
    130                 135                 140                 


Gln Ala Asn His Ser His Ser Gly Asp Tyr His Cys Thr Gly Asn Ile 
145                 150                 155                 160 


Gly Tyr Thr Leu Tyr Ser Ser Lys Pro Val Thr Ile Thr Val Gln Ala 
                165                 170                 175     


Pro Ser Ser Ser Pro 
            180     


<210>  6
<211>  329
<212>  PRT
<213>  Artificial

<220>
<223>  humanized antibody


<220>
<221>  MISC_FEATURE
<222>  (1)..(329)
<223>  CH hu8A6_wt

<400>  6

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
225                 230                 235                 240 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly 
                325                 


<210>  7
<211>  106
<212>  PRT
<213>  Artificial

<220>
<223>  humanized antibody


<220>
<221>  MISC_FEATURE
<222>  (1)..(106)
<223>  CL hu8A6_wt

<400>  7

Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
1               5                   10                  15      


Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
            20                  25                  30          


Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
        35                  40                  45              


Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
    50                  55                  60                  


Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
65                  70                  75                  80  


His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro 
                85                  90                  95      


Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105     


<210>  8
<211>  339
<212>  DNA
<213>  Artificial

<220>
<223>  humanized antibody


<220>
<221>  misc_feature
<222>  (1)..(339)
<223>  VH hu8A6

<400>  8
caggtgcagc tggtggagtc tgggggaggc gtggtccagc ctgggaggtc cctgagactc     60

tcctgtgcag cctctggatt caccttcagt gactattaca tggcctgggt ccgccaggct    120

ccaggcaagg ggctggagtg ggtggcatcc atatcatacg atggaagcaa taagtactac    180

ggagactccg tgaagggccg attcaccatc tccagagaca attccaagaa cacgctgtat    240

ctgcaaatga acagcctgag agctgaggac acggctgtgt attactgtgc gagaccggga    300

gactactggg gccaaggaac cctggtcacc gtcagctca                           339


<210>  9
<211>  324
<212>  DNA
<213>  Artificial

<220>
<223>  humanized antibody


<220>
<221>  misc_feature
<222>  (1)..(324)
<223>  VL hu8A6

<400>  9
cagatagtga tgacgcagtc tccagccacc ctgtctgtgt ctccagggga aagagccacc     60

ctctcctgca gggccagtca gtccgttggc tcctatgtcg actggtacca gcagaaacct    120

ggccaggctc ccaggctcct catctatggt gcatccacca ggtacactgg tatcccagcc    180

aggttcagtg gcagtgggtc tgggacagag ttcactctca ccatcagcag cctgcagtct    240

gaagattttg cagtttatta ctgtctgcag tataacaacc atccttacac ttttggccag    300

gggaccaagc tggagatcaa acgt                                           324


<210>  10
<211>  990
<212>  DNA
<213>  Artificial

<220>
<223>  humanized antibody


<220>
<221>  misc_feature
<222>  (1)..(990)
<223>  CH hu8A6_wt

<400>  10
gcctccacca agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg     60

ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg    120

tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca    180

ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc    240

tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa ggttgagccc    300

aaatcttgtg acaaaactca cacatgccca ccgtgcccag cacctgaact cctgggggga    360

ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc ccggacccct    420

gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg    480

tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga gcagtacaac    540

agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct gaatggcaag    600

gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa aaccatctcc    660

aaagccaaag ggcagccccg agaaccacag gtgtacaccc tgcccccatc ccgggaggag    720

atgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc cagcgacatc    780

gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac gcctcccgtg    840

ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa gagcaggtgg    900

cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa ccactacacg    960

cagaagagcc tctccctgtc tccgggttaa                                     990


<210>  11
<211>  321
<212>  DNA
<213>  Artificial

<220>
<223>  humanized antibody


<220>
<221>  misc_feature
<222>  (1)..(321)
<223>  CL hu8A6_wt

<400>  11
acggtggctg caccatcggt cttcatcttc ccgccatctg atgagcagtt gaaatctgga     60

actgcctctg ttgtgtgcct gctgaataac ttctatccca gagaggccaa agtacagtgg    120

aaggtggata acgccctcca atcgggtaac tcccaggaga gtgtcacaga gcaggacagc    180

aaggacagca cctacagcct cagcagcacc ctgacgctga gcaaagcaga ctacgagaaa    240

cacaaagtct acgcctgcga agtcacccat cagggcctga gctcgcccgt cacaaagagc    300

ttcaacaggg gagagtgtta g                                              321


<210>  12
<211>  125
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(125)
<223>  hu soluble FCyRIIA

<400>  12

Arg Phe Lys Ala Asn Asn Asn Asp Ser Gly Glu Tyr Thr Cys Gln Thr 
1               5                   10                  15      


Gly Gln Thr Ser Leu Ser Asp Pro Val His Leu Thr Val Leu Ser Glu 
            20                  25                  30          


Trp Leu Val Leu Gln Thr Pro His Leu Glu Phe Gln Glu Gly Glu Thr 
        35                  40                  45              


Ile Met Leu Arg Cys His Ser Trp Lys Asp Lys Pro Leu Val Lys Val 
    50                  55                  60                  


Thr Phe Phe Gln Asn Gly Lys Ser Gln Lys Phe Ser Arg Leu Asp Pro 
65                  70                  75                  80  


Thr Phe Ser Ile Pro Gln Ala Asn His Ser His Ser Gly Asp Tyr His 
                85                  90                  95      


Cys Thr Gly Asn Ile Gly Tyr Thr Leu Phe Ser Ser Lys Pro Val Thr 
            100                 105                 110         


Ile Thr Val Gln Val Pro Ser Met Gly Ser Ser Ser Pro 
        115                 120                 125 


<210>  13
<211>  179
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<222>  (1)..(179)
<223>  soluble mutated human FCyRIIA

<400>  13

Met Ala Pro Pro Lys Ala Val Leu Lys Leu Glu Pro Pro Trp Ile Asn 
1               5                   10                  15      


Val Leu Gln Glu Asp Ser Val Thr Leu Thr Cys Gln Gly Ala Arg Ser 
            20                  25                  30          


Pro Glu Ser Asp Ser Ile Gln Trp Phe His Asn Gly Asn Leu Ile Pro 
        35                  40                  45              


Thr His Thr Gln Pro Ser Tyr Arg Phe Lys Ala Asn Asn Asn Asp Ser 
    50                  55                  60                  


Gly Glu Tyr Thr Cys Gln Thr Gly Gln Thr Ser Leu Ser Asp Pro Val 
65                  70                  75                  80  


His Leu Thr Val Leu Ser Glu Trp Leu Val Leu Gln Thr Pro His Leu 
                85                  90                  95      


Glu Phe Gln Glu Gly Glu Thr Ile Met Leu Arg Cys His Ser Trp Lys 
            100                 105                 110         


Asp Lys Pro Leu Val Lys Val Thr Phe Phe Gln Asn Gly Lys Ser Lys 
        115                 120                 125             


Lys Phe Ser Arg Ser Asp Pro Asn Phe Ser Ile Pro Gln Ala Asn His 
    130                 135                 140                 


Ser His Ser Gly Asp Tyr His Cys Thr Gly Asn Ile Gly Tyr Thr Leu 
145                 150                 155                 160 


Phe Ser Ser Lys Pro Val Thr Ile Thr Val Gln Val Pro Ser Met Gly 
                165                 170                 175     


Ser Ser Pro 
            

                         
<210>  14
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  heavy chain CDR1 from rat antibody 8A6

<400>  14

Asp Tyr Tyr Met Ala 
1               5   


<210>  15
<211>  17
<212>  PRT
<213>  artificial

<220>
<223>  heavy chain CDR2 from rat antibody 8A6

<400>  15

Ser Ile Ser Ser Asp Gly Ser Asn Thr Tyr Tyr Gly Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  16
<211>  6
<212>  PRT
<213>  artificial

<220>
<223>  heavy chain CDR3 from rat antibody 8A6

<400>  16

Ala Arg Pro Gly Asp Tyr 
1               5       


<210>  17
<211>  11
<212>  PRT
<213>  artificial

<220>
<223>  light chain CDR1 from rat antibody 8A6

<400>  17

Lys Ala Ser Gln Asn Val Gly Thr Tyr Val Asp 
1               5                   10      


<210>  18
<211>  7
<212>  PRT
<213>  artificial

<220>
<223>  light chain CDR2 from rat antibody 8A6

<400>  18

Gly Ala Ser Asn Arg Tyr Thr 
1               5           


<210>  19
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  light chain CDR3 from rat antibody 8A6

<400>  19

Leu Gln Tyr Asn Tyr His Pro Tyr Thr 
1               5                   


<210>  20
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  heavy chain CDR1 from humanized antibody 8A6

<400>  20

Asp Tyr Tyr Met Ala 
1               5   


<210>  21
<211>  17
<212>  PRT
<213>  artificial

<220>
<223>  heavy chain CDR2 from humanized antibody 8A6

<400>  21

Ser Ile Ser Tyr Asp Gly Ser Asn Lys Tyr Tyr Gly Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  22
<211>  6
<212>  PRT
<213>  artificial

<220>
<223>  heavy chain CDR3 from humanized antibody 8A6

<400>  22

Ala Arg Pro Gly Asp Tyr 
1               5       


<210>  23
<211>  11
<212>  PRT
<213>  artificial

<220>
<223>  light chain CDR1 from humanized antibody 8A6

<400>  23

Arg Ala Ser Gln Ser Val Gly Ser Tyr Val Asp 
1               5                   10      


<210>  24
<211>  7
<212>  PRT
<213>  artificial

<220>
<223>  light chain CDR2 from humanized antibody 8A6

<400>  24

Gly Ala Ser Thr Arg Tyr Thr 
1               5           


<210>  25
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  light chain CDR3 from humanized antibody 8A6

<400>  25

Leu Gln Tyr Asn Asn His Pro Tyr Thr 
1               5                   


<210>  26
<211>  104
<212>  PRT
<213>  artificial

<220>
<223>  variable region of the heavy chain of antibody GB3

<400>  26

Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala Ser 
1               5                   10                  15      


Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr Tyr 
            20                  25                  30          


Ile Tyr Trp Val Lys Gln Trp Pro Gly Gln Gly Leu Glu Trp Ile Gly 
        35                  40                  45              


Trp Ile Phe Pro Gly Thr Gly Asn Thr Tyr Tyr Asn Glu Asn Phe Lys 
    50                  55                  60                  


Asp Lys Ala Thr Leu Thr Ile Asp Arg Ser Ser Ser Thr Ala Tyr Met 
65                  70                  75                  80  


Leu Leu Gly Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Tyr 
                85                  90                  95      


Gly Pro Phe Ala Tyr Trp Gly Gln 
            100                 


<210>  27
<211>  104
<212>  PRT
<213>  artificial

<220>
<223>  variable region of the light chain of the antbody GB3

<400>  27

Arg Ile Gln Leu Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Glu Arg Val Ser Leu Thr Cys Arg Ala Ser Gln Glu Ile Ser Gly Tyr 
            20                  25                  30          


Leu Ser Trp Leu Gln Gln Lys Pro Asp Gly Thr Ile Lys Arg Leu Ile 
        35                  40                  45              


Tyr Ala Thr Ser Ala Leu Asp Ser Gly Val Pro Lys Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Ser Asn Tyr Ser Leu Thr Ile Ser Ser Leu Glu Ser 
65                  70                  75                  80  


Glu Asp Phe Ala Asp Tyr Tyr Cys Leu Gln Tyr Ala Asn Tyr Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu 
            100                 


<210>  28
<211>  329
<212>  PRT
<213>  artificial

<220>
<223>  IgG heavy chain constant region with N to A change at position 
       297 when regarding pos. 1 as pos. 118

<400>  28

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Ala Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
225                 230                 235                 240 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly 
                325                 


<210>  29
<211>  5
<212>  PRT
<213>  artificial

<220>
<223>  CDR1 from heavy chain variable region


<220>
<221>  misc
<222>  (1)..(1)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (1)..(1)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (2)..(2)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (3)..(3)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (3)..(3)
<223>  Xaa can be any amino acid

<400>  29

Xaa Xaa Xaa Met Ala 
1               5   


<210>  30
<211>  17
<212>  PRT
<213>  artificial

<220>
<223>  CDR2 from heavy chain variable region


<220>
<221>  misc
<222>  (2)..(2)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (2)..(2)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (8)..(8)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (9)..(9)
<223>  Xaa can K or T

<220>
<221>  misc
<222>  (10)..(10)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (11)..(11)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (16)..(16)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (17)..(17)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (17)..(17)
<223>  Xaa can be any amini acid

<400>  30

Ser Xaa Ser Tyr Asp Gly Ser Xaa Xaa Xaa Xaa Gly Asp Ser Val Xaa 
1               5                   10                  15      


Xaa 
    


<210>  31
<211>  6
<212>  PRT
<213>  artificial

<220>
<223>  CDR3 from heavy chain variable region


<220>
<221>  misc
<222>  (3)..(3)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (3)..(3)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (5)..(5)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (6)..(6)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (6)..(6)
<223>  Xaa can be any amino acid

<400>  31

Ala Arg Xaa Gly Xaa Xaa 
1               5       


<210>  32
<211>  11
<212>  PRT
<213>  artificial

<220>
<223>  CDR1 from light chain variable region


<220>
<221>  misc
<222>  (1)..(1)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (2)..(2)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (3)..(3)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (4)..(4)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (5)..(5)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (7)..(7)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (8)..(8)
<223>  Xaa can be S or T

<220>
<221>  misc
<222>  (9)..(9)
<223>  Xaa can be any amino acid

<400>  32

Xaa Xaa Xaa Xaa Xaa Val Xaa Xaa Xaa Val Asp 
1               5                   10      


<210>  33
<211>  7
<212>  PRT
<213>  artificial

<220>
<223>  CDR2 from light chain variable region


<220>
<221>  misc
<222>  (2)..(2)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (3)..(3)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (4)..(4)
<223>  Xaa can be T or N

<400>  33

Gly Xaa Xaa Xaa Arg Tyr Thr 
1               5           


<210>  34
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  CDR3 from light chain variable region


<220>
<221>  misc
<222>  (1)..(1)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (1)..(1)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (2)..(2)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (3)..(3)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (4)..(4)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (7)..(7)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (8)..(8)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (9)..(9)
<223>  Xaa can be any amino acid

<220>
<221>  misc
<222>  (9)..(9)
<223>  Xaa can be any amino acid

<400>  34

Xaa Xaa Xaa Xaa Asn His Xaa Xaa Xaa 
1               5                   



<210>  35
<211>  433
<212>  PRT
<213>  artificial

<220>
<223>  human IgE constant region

<400>  35

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Pro Lys Ser Cys Asp Lys Thr Phe Ser Val Ser Ser Arg 
            100                 105                 110         


Asp Phe Thr Pro Pro Thr Val Lys Ile Leu Gln Ser Ser Cys Asp Gly 
        115                 120                 125             


Gly Gly His Phe Pro Pro Thr Ile Gln Leu Leu Cys Leu Val Ser Gly 
    130                 135                 140                 


Tyr Thr Pro Gly Thr Ile Asn Ile Thr Trp Leu Glu Asp Gly Gln Val 
145                 150                 155                 160 


Met Asp Val Asp Leu Ser Thr Ala Ser Thr Thr Gln Glu Gly Glu Leu 
                165                 170                 175     


Ala Ser Thr Gln Ser Glu Leu Thr Leu Ser Gln Lys His Trp Leu Ser 
            180                 185                 190         


Asp Arg Thr Tyr Thr Cys Gln Val Thr Tyr Gln Gly His Thr Phe Glu 
        195                 200                 205             


Asp Ser Thr Lys Lys Cys Ala Asp Ser Asn Pro Arg Gly Val Ser Ala 
    210                 215                 220                 


Tyr Leu Ser Arg Pro Ser Pro Phe Asp Leu Phe Ile Arg Lys Ser Pro 
225                 230                 235                 240 


Thr Ile Thr Cys Leu Val Val Asp Leu Ala Pro Ser Lys Gly Thr Val 
                245                 250                 255     


Asn Leu Thr Trp Ser Arg Ala Ser Gly Lys Pro Val Asn His Ser Thr 
            260                 265                 270         


Arg Lys Glu Glu Lys Gln Arg Asn Gly Thr Leu Thr Val Thr Ser Thr 
        275                 280                 285             


Leu Pro Val Gly Thr Arg Asp Trp Ile Glu Gly Glu Thr Tyr Gln Cys 
    290                 295                 300                 


Arg Val Thr His Pro His Leu Pro Arg Ala Leu Met Arg Ser Thr Thr 
305                 310                 315                 320 


Lys Thr Ser Gly Pro Arg Ala Ala Pro Glu Val Tyr Ala Phe Ala Thr 
                325                 330                 335     


Pro Glu Trp Pro Gly Ser Arg Asp Lys Arg Thr Leu Ala Cys Leu Ile 
            340                 345                 350         


Gln Asn Phe Met Pro Glu Asp Ile Ser Val Gln Trp Leu His Asn Glu 
        355                 360                 365             


Val Gln Leu Pro Asp Ala Arg His Ser Thr Thr Gln Pro Arg Lys Thr 
    370                 375                 380                 


Lys Gly Ser Gly Phe Phe Val Phe Ser Arg Leu Glu Val Thr Arg Ala 
385                 390                 395                 400 


Glu Trp Glu Gln Lys Asp Glu Phe Ile Cys Arg Ala Val His Glu Ala 
                405                 410                 415     


Ala Ser Pro Ser Gln Thr Val Gln Arg Ala Val Ser Val Asn Pro Gly 
            420                 425                 430         


Lys 
    


<210>  36
<211>  332
<212>  PRT
<213>  artificial

<220>
<223>  constant IgE domains 3 and 4

<400>  36

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Val Ser Ala Tyr Leu Ser Arg Pro 
        115                 120                 125             


Ser Pro Phe Asp Leu Phe Ile Arg Lys Ser Pro Thr Ile Thr Cys Leu 
    130                 135                 140                 


Val Val Asp Leu Ala Pro Ser Lys Gly Thr Val Asn Leu Thr Trp Ser 
145                 150                 155                 160 


Arg Ala Ser Gly Lys Pro Val Asn His Ser Thr Arg Lys Glu Glu Lys 
                165                 170                 175     


Gln Arg Asn Gly Thr Leu Thr Val Thr Ser Thr Leu Pro Val Gly Thr 
            180                 185                 190         


Arg Asp Trp Ile Glu Gly Glu Thr Tyr Gln Cys Arg Val Thr His Pro 
        195                 200                 205             


His Leu Pro Arg Ala Leu Met Arg Ser Thr Thr Lys Thr Ser Gly Pro 
    210                 215                 220                 


Arg Ala Ala Pro Glu Val Tyr Ala Phe Ala Thr Pro Glu Trp Pro Gly 
225                 230                 235                 240 


Ser Arg Asp Lys Arg Thr Leu Ala Cys Leu Ile Gln Asn Phe Met Pro 
                245                 250                 255     


Glu Asp Ile Ser Val Gln Trp Leu His Asn Glu Val Gln Leu Pro Asp 
            260                 265                 270         


Ala Arg His Ser Thr Thr Gln Pro Arg Lys Thr Lys Gly Ser Gly Phe 
        275                 280                 285             


Phe Val Phe Ser Arg Leu Glu Val Thr Arg Ala Glu Trp Glu Gln Lys 
    290                 295                 300                 


Asp Glu Phe Ile Cys Arg Ala Val His Glu Ala Ala Ser Pro Ser Gln 
305                 310                 315                 320 


Thr Val Gln Arg Ala Val Ser Val Asn Pro Gly Lys 
                325                 330         

<210>  37
<211>  600
<212>  DNA
<213>  artificial

<220>
<223>  Plasmid DNA Sequence of soluble Fc epsilon RI

<400>  37
atgggtgaca atgacatcca ctttgccttt ctctccacag gtgtccagtc cgtccctcag       60

aaacctaagg tctccttgaa ccctccatgg aatagaatat ttaaaggaga gaatgtgact      120

cttacatgta atgggaacaa tttctttgaa gtcagttcca ccaaatggtt ccacaatggc      180

agcctttcag aagagacaaa ttcaagtttg aatattgtga atgccaaatt tgaagacagt      240

ggagaataca aatgtcagca ccaacaagtt aatgagagtg aacctgtgta cctggaagtc      300

ttcagtgact ggctgctcct tcaggcctct gctgaggtgg tgatggaggg ccagcccctc      360

ttcctcaggt gccatggttg gaggaactgg gatgtgtaca aggtgatcta ttataaggat      420

ggtgaagctc tcaagtactg gtatgagaac cacaacatct ccattacaaa tgccacagtt      480

gaagacagtg gaacctacta ctgtacgggc aaagtgtggc agctggacta tgagtctgag      540

cccctcaaca ttactgtaat aaaagctccg cgtgagaagc accatcacca ccatcactga      600


<210>  38
<211>  3
<212>  PRT
<213>  artificial

<220>
<223>  fMLF peptide


<220>
<221>  MOD_RES
<222>  (1)..(1)
<223>  FORMYLATION

<400>  38

Met Leu Phe 
1
      



       



