                         SEQUENCE LISTING

<110>  Rockefeller University
 
<120>  COMPOSITIONS AND METHODS FOR TRANSCRIPTION-BASED CRISPR-CAS DNA 
       EDITING

<130>  076091.00014

<150>  62/034,684
<151>  2014-08-07

<160>  192   

<170>  PatentIn version 3.5

<210>  1
<211>  4627
<212>  DNA
<213>  artificial sequence

<220>
<223>  Plasmid

<400>  1
ctctgtaact gcttatttgc cttctgtaac tcatccttaa cttcttgcag ttcctgttta       60

tgaaatacag tatctttctt gtactgatcc atcgctttat gttctcgttc tgtaacctct      120

ttggacgtgc ctctttcaag ttcataacct ttctcattca catactcatt aaatctatct      180

tgtaattgag taaagtcttt cttgttgcct aactgttctt ttgcagacaa tctcccgtcc      240

tctgttaaag ggacaaaacc aaagtgcata tgtgggactc tttcatccag atggacagtc      300

gcatacagca tattttcctt accgtattca ttttctagaa actccaagct atctttaaaa      360

aatcgttcta tttcttctcc gcttaaatca tcaaagaaat ctttatcact tgtaaccagt      420

ccgtccacat gtcgaattgc atctgaccga attttacgtt tccctgaata attctcatca      480

atcgtttcat caattttatc tttatacttt atattttgtg cgttaatcaa atcataattt      540

ttatatgttt cctcatgatt tatgtcttta ttattatagt ttttattctc tctttgatta      600

tgtctttgta tcccgtttgt attacttgat cctttaactc tggcaaccct caaaattgaa      660

tgagacatgc tacacctccg gataataaat atatataaac gtatatagat ttcataaagt      720

ctaacacact agacttattt acttcgtaat taagtcgtta aaccgtgtgc tctacgacca      780

aaactataaa acctttaaga actttctttt tttacaagaa aaaagaaatt agataaatct      840

ctcatatctt ttattcaata atcgcatccg attgcagtat aaatttaacg atcactcgtt      900

accatcacgg aaaaaggtta tgctgctttt aagacccact ttcacattta agttgttttt      960

ctaatccgca tatgatcaat tcaaggccga ataagaaggc tggctctgca ccttggtgat     1020

caaataattc gatagcttgt cgtaataatg gcggcatact atcagtagta ggtgtttccc     1080

tttcttcttt agcgacttga tgctcttgat cttccaatac gcaacctaaa gtaaaatgcc     1140

ccacagcgct gagtgcatat aatgcattct ctagtgaaaa accttgttgg cataaaaagg     1200

ctaattgatt ttcgagagtt tcatactgtt tttctgtagg ccgtgtacct aaatgtactt     1260

ttgctccatc gcgatgactt agtaaagcac atctaaaact tttagcgtta ttacgtaaaa     1320

aatcttgcca gctttcccct tctaaagggc aaaagtgagt atggtgccta tctaacatct     1380

caatggctaa ggcgtcgagc aaagcccgct tattttttac atgccaatac aatgtaggct     1440

gctctacacc tagcttctgg gcgagtttac gggttgttaa accttcgatt ccgacctcat     1500

taagcagctc taatgcgctg ttaatcactt tacttttatc taatctagac atcattaatt     1560

cctccttttt gttgacatta tatcattgat agagttattt gtcaaactag ttttttattt     1620

ggatcccctc gagttcatga aaaactaaaa aaaatattga cactctatca ttgatagagc     1680

ataattaaaa taagctctct atcattgata gagtgatatc cggaggcata tcaaatgacc     1740

taggagatct gtgattctta cctttgtact gatgatttat atacttcggc atacgttctc     1800

taaatatctt gttcatattt atcagagctc gtgctataat tatactaatt ttataaggag     1860

gaaaaaatat gggcattttt agtatttttg taatcagcac agttcattat caaccaaaca     1920

aaaaataagt ggttataatg aatcgttaat aagcaaaatt catataacca aattaaagag     1980

ggttataatg aacgagaaaa atataaaaca cagtcaaaac tttattactt caaaacataa     2040

tatagataaa ataatgacaa atataagatt aaatgaacat gataatatct ttgaaatcgg     2100

ctcaggaaaa ggccatttta cccttgaatt agtaaagagg tgtaatttcg taactgccat     2160

tgaaatagac cataaattat gcaaaactac agaaaataaa cttgttgatc acgataattt     2220

ccaagtttta aacaaggata tattgcagtt taaatttcct aaaaaccaat cctataaaat     2280

atatggtaat ataccttata acataagtac ggatataata cgcaaaattg tttttgatag     2340

tatagctaat gagatttatt taatcgtgga atacgggttt gctaaaagat tattaaatac     2400

aaaacgctca ttggcattac ttttaatggc agaagttgat atttctatat taagtatggt     2460

tccaagagaa tattttcatc ctaaacctaa agtgaatagc tcacttatca gattaagtag     2520

aaaaaaatca agaatatcac acaaagataa acaaaagtat aattatttcg ttatgaaatg     2580

ggttaacaaa gaatacaaga aaatatttac aaaaaatcaa tttaacaatt ccttaaaaca     2640

tgcaggaatt gacgatttaa acaatattag ctttgaacaa ttcttatctc ttttcaatag     2700

ctataaatta tttaataagt aagttaaggg atgcataaac tgcatccctt aacttgtttt     2760

tcgtgtgcct attttttgtg aatcgattat gtcttttgcg cagtcggctt aaaccagttt     2820

tcgctggtgc gaaaaaagag tgtcttgtga cacctaaatt caaaatctat cggtcagatt     2880

tataccgatt tgattttata tattcttgaa taacatacgc cgagttatca cataaaagcg     2940

ggaaccaatc atcaaattta aacttcattg cataatccat taaactctta aattctacga     3000

ttccttgttc atcaataaac tcaatcattt ctttaattaa tttatatcta tctgttgttg     3060

ttttctttaa taattcatca acatctacac cgccataaac tatcatatct tctttttgat     3120

atttaaattt attaggatcg tccatgtgaa gcatatatct cacaagacct ttcacacttc     3180

ctgcaatctg cggaatagtc gcattcaatt cttctgttaa ttatttttat ctgttcataa     3240

gatttattac cctcatacat cactagaata tgataatgct cttttttcat cctaccttct     3300

gtatcagtat ccctatcatg taatggagac actacaaatt gaatgtgtaa ctcttttaaa     3360

tactctaacc actcggcttt tgctgattct ggatataaaa caaatgtcca attacgtcct     3420

cttgaatttt tcttgttttc agtttctttt attacatttt cgctcatgat ataataacgg     3480

tgctaataca cttaacaaaa tttagtcata gataggcagc atgccagtgc tgtctatctt     3540

tttttgttta aaatgcaccg tattcctcct ttgcatattt ttttattaga ataccggttg     3600

catctgattt gctaatatta tatttttctt tgattctatt taatatctca ttttcttctg     3660

ttgtaagtct taaagtaaca gcaacttttt tctcttcttt tctatctaca accatcactg     3720

tacctcccaa catctgtttt tttcacttta acataaaaaa caacctttta acattaaaaa     3780

cccaatattt atttatttgt ttggacaatg gacaatggac acctaggggg gaggtcgtag     3840

taccccccta tgttttctcc cctaaataac cccaaaaatc taagaaaaaa agacctcaaa     3900

aaggtcttta attaacatct caaatttcgc atttattcca atttcctttt tgcgtgtgat     3960

gcgctgcgtc cattaaaaat cctagagctt tgcaaccgaa agttaatagc tgtcgctact     4020

actttcgctt acgctctaag tatattttaa ggactgtcac acgcaaaaag ttttctcggc     4080

ataaaagtac ctctacatct ctaaatcgtc tgtacgctgt ttctcacgct ttctatcgac     4140

cttctggaca ttatcctgta caacatccat aaactgtccc acacgctcaa atttggaatc     4200

attaaagaat ttctctttaa gcctattaaa ccctttctca aacccaggga aattcgccct     4260

cgcagcacga tataaagtca ctgtactagc ttgaaatttc tctgatacat tcaactgctc     4320

attcaaacta tcattctctc gctttaattt attaacctct ttactttttt cgtgataccc     4380

ctctttccat gtattcacta cttctttcaa actctctcta cgttttttta attcttgatt     4440

ttctgtgtaa tagtctgtgc tcttaatatt ttcgtaatca tcaacaatcc gttctgcaga     4500

agagattgtt tcttgcaggc gttcaaattc atcagcagtt aatatctttc taccagtctc     4560

ttcacgtcca gagaacaaac ctgtacgctc attttcataa tcaaagggtt tcgtagacct     4620

catatgc                                                               4627


<210>  2
<211>  757
<212>  PRT
<213>  artificial sequence

<220>
<223>  CRISPR associated enzyme

<400>  2

Met Asn Lys Lys Asn Ile Leu Met Tyr Gly Ser Leu Leu His Asp Ile 
1               5                   10                  15      


Gly Lys Ile Ile Tyr Arg Ser Gly Asp His Thr Phe Ser Arg Gly Thr 
            20                  25                  30          


His Ser Lys Leu Gly His Gln Phe Leu Ser Gln Phe Ser Glu Phe Lys 
        35                  40                  45              


Asp Asn Glu Val Leu Asp Asn Val Ala Tyr His His Tyr Lys Glu Leu 
    50                  55                  60                  


Ala Lys Ala Asn Leu Asp Asn Asp Asn Thr Ala Tyr Ile Thr Tyr Ile 
65                  70                  75                  80  


Ala Asp Asn Ile Ala Ser Gly Ile Asp Arg Arg Asp Ile Ile Glu Glu 
                85                  90                  95      


Gly Asp Glu Glu Tyr Glu Lys Gln Leu Phe Asn Phe Asp Lys Tyr Thr 
            100                 105                 110         


Pro Leu Tyr Ser Val Phe Asn Ile Val Asn Ser Glu Lys Leu Lys Gln 
        115                 120                 125             


Thr Asn Gly Lys Phe Lys Phe Ser Asn Glu Ser Asn Ile Glu Tyr Pro 
    130                 135                 140                 


Lys Thr Glu Asn Ile Gln Tyr Ser Ser Gly Asn Tyr Thr Thr Leu Met 
145                 150                 155                 160 


Lys Asp Met Ser His Asp Leu Glu His Lys Leu Ser Ile Lys Glu Gly 
                165                 170                 175     


Thr Phe Pro Ser Leu Leu Gln Trp Thr Glu Ser Leu Trp Gln Tyr Val 
            180                 185                 190         


Pro Ser Ser Thr Asn Lys Asn Gln Leu Ile Asp Ile Ser Leu Tyr Asp 
        195                 200                 205             


His Ser Arg Ile Thr Cys Ala Ile Ala Ser Cys Ile Phe Asp Tyr Leu 
    210                 215                 220                 


Asn Glu Asn Asn Ile His Asn Tyr Lys Asp Glu Leu Phe Ser Lys Tyr 
225                 230                 235                 240 


Glu Asn Thr Lys Ser Phe Tyr Gln Lys Glu Ala Phe Leu Leu Leu Ser 
                245                 250                 255     


Met Asp Met Ser Gly Ile Gln Asp Phe Ile Tyr Asn Ile Ser Gly Ser 
            260                 265                 270         


Lys Ala Leu Lys Ser Leu Arg Ser Arg Ser Phe Tyr Leu Glu Leu Met 
        275                 280                 285             


Leu Glu Val Ile Val Asp Gln Leu Leu Glu Arg Leu Glu Leu Ala Arg 
    290                 295                 300                 


Ala Asn Leu Leu Tyr Thr Gly Gly Gly His Ala Tyr Leu Leu Val Ser 
305                 310                 315                 320 


Asn Thr Asp Lys Val Lys Lys Lys Ile Thr Gln Phe Asn Asn Glu Leu 
                325                 330                 335     


Lys Lys Trp Phe Met Ser Glu Phe Thr Thr Asp Leu Ser Leu Ser Met 
            340                 345                 350         


Ala Phe Glu Lys Cys Ser Gly Asp Asp Leu Met Asn Thr Ser Gly Asn 
        355                 360                 365             


Tyr Arg Thr Ile Trp Arg Asn Val Ser Ser Lys Leu Ser Asp Ile Lys 
    370                 375                 380                 


Ala His Lys Tyr Ser Ala Glu Asp Ile Leu Lys Leu Asn His Phe His 
385                 390                 395                 400 


Ser Tyr Gly Asp Arg Glu Cys Lys Glu Cys Leu Arg Ser Asp Ile Asp 
                405                 410                 415     


Ile Asn Asp Asp Gly Leu Cys Ser Ile Cys Glu Gly Ile Ile Asn Ile 
            420                 425                 430         


Ser Asn Asp Leu Arg Asp Lys Ser Phe Phe Val Leu Ser Glu Thr Gly 
        435                 440                 445             


Lys Leu Lys Met Pro Phe Asn Lys Phe Ile Ser Val Ile Asp Tyr Glu 
    450                 455                 460                 


Glu Ala Glu Met Leu Val Gln Asn Asn Asn Gln Val Arg Ile Tyr Ser 
465                 470                 475                 480 


Lys Asn Lys Pro Tyr Ile Gly Ile Gly Ile Ser Thr Asn Leu Trp Met 
                485                 490                 495     


Cys Asp Tyr Asp Tyr Ala Ser Gln Asn Gln Asp Met Arg Glu Lys Gly 
            500                 505                 510         


Ile Gly Ser Tyr Val Asp Arg Glu Glu Gly Val Lys Arg Leu Gly Val 
        515                 520                 525             


Val Arg Ala Asp Ile Asp Asn Leu Gly Ala Thr Phe Ile Ser Gly Ile 
    530                 535                 540                 


Pro Glu Lys Tyr Asn Ser Ile Ser Arg Thr Ala Thr Leu Ser Arg Gln 
545                 550                 555                 560 


Leu Ser Leu Phe Phe Lys Tyr Glu Leu Asn His Leu Leu Glu Asn Tyr 
                565                 570                 575     


Gln Ile Thr Ala Ile Tyr Ser Gly Gly Asp Asp Leu Phe Leu Ile Gly 
            580                 585                 590         


Ala Trp Asp Asp Ile Ile Glu Ala Ser Ile Tyr Ile Asn Asp Lys Phe 
        595                 600                 605             


Lys Glu Phe Thr Leu Asp Lys Leu Thr Leu Ser Ala Gly Val Gly Met 
    610                 615                 620                 


Phe Ser Gly Lys Tyr Pro Val Ser Lys Met Ala Phe Glu Thr Gly Arg 
625                 630                 635                 640 


Leu Glu Glu Ala Ala Lys Thr Gly Glu Lys Asn Gln Ile Ser Leu Trp 
                645                 650                 655     


Leu Gln Glu Lys Val Tyr Asn Trp Asp Glu Phe Lys Lys Asn Ile Leu 
            660                 665                 670         


Glu Glu Lys Leu Leu Val Leu Gln Gln Gly Phe Ser Gln Thr Asp Glu 
        675                 680                 685             


His Gly Lys Ala Phe Ile Tyr Lys Met Leu Ala Leu Leu Arg Asn Asn 
    690                 695                 700                 


Glu Ala Ile Asn Ile Ala Arg Leu Ala Tyr Leu Leu Ala Arg Ser Lys 
705                 710                 715                 720 


Met Asn Glu Asp Phe Thr Ser Lys Ile Phe Asn Trp Ala Gln Asn Asp 
                725                 730                 735     


Lys Asp Lys Asn Gln Leu Ile Thr Ala Leu Glu Tyr Tyr Ile Tyr Gln 
            740                 745                 750         


Ile Arg Glu Ala Asp 
        755         


<210>  3
<211>  141
<212>  PRT
<213>  artificial sequence

<220>
<223>  CRISPR associated enzyme

<400>  3

Met Ile Leu Ala Lys Thr Lys Ser Gly Lys Thr Ile Asp Leu Thr Phe 
1               5                   10                  15      


Ala His Glu Val Val Lys Ser Asn Val Lys Asn Val Lys Asp Arg Lys 
            20                  25                  30          


Gly Lys Glu Lys Gln Val Leu Phe Asn Gly Leu Thr Thr Ser Lys Leu 
        35                  40                  45              


Arg Asn Leu Met Glu Gln Val Asn Arg Leu Tyr Thr Ile Ala Phe Asn 
    50                  55                  60                  


Ser Asn Glu Asp Gln Leu Asn Glu Glu Phe Ile Asp Glu Leu Glu Tyr 
65                  70                  75                  80  


Leu Lys Ile Lys Phe Tyr Tyr Glu Ala Gly Arg Glu Lys Ser Val Asp 
                85                  90                  95      


Glu Phe Leu Lys Lys Thr Leu Met Phe Pro Ile Ile Asp Arg Val Ile 
            100                 105                 110         


Lys Lys Glu Ser Lys Lys Phe Phe Leu Asp Tyr Cys Lys Tyr Phe Glu 
        115                 120                 125             


Ala Leu Val Ala Tyr Ala Lys Tyr Tyr Gln Lys Glu Asp 
    130                 135                 140     


<210>  4
<211>  214
<212>  PRT
<213>  artificial sequence

<220>
<223>  CRISPR-associated enzyme

<400>  4

Met Tyr Ser Lys Ile Lys Ile Ser Gly Thr Ile Glu Val Val Thr Gly 
1               5                   10                  15      


Leu His Ile Gly Gly Gly Gly Glu Ser Ser Met Ile Gly Ala Ile Asp 
            20                  25                  30          


Ser Pro Val Val Arg Asp Leu Gln Thr Lys Leu Pro Ile Ile Pro Gly 
        35                  40                  45              


Ser Ser Ile Lys Gly Lys Met Arg Asn Leu Leu Ala Lys His Phe Gly 
    50                  55                  60                  


Leu Lys Met Lys Gln Glu Ser His Asn Gln Asp Asp Glu Arg Val Leu 
65                  70                  75                  80  


Arg Leu Phe Gly Ser Ser Glu Lys Gly Asn Ile Gln Arg Ala Arg Leu 
                85                  90                  95      


Gln Ile Ser Asp Ala Phe Phe Ser Glu Lys Thr Lys Glu His Phe Ala 
            100                 105                 110         


Gln Asn Asp Ile Ala Tyr Thr Glu Thr Lys Phe Glu Asn Thr Ile Asn 
        115                 120                 125             


Arg Leu Thr Ala Val Ala Asn Pro Arg Gln Ile Glu Arg Val Thr Arg 
    130                 135                 140                 


Gly Ser Glu Phe Asp Phe Val Phe Ile Tyr Asn Val Asp Glu Glu Ser 
145                 150                 155                 160 


Gln Val Glu Asp Asp Phe Glu Asn Ile Glu Lys Ala Ile His Leu Leu 
                165                 170                 175     


Glu Asn Asp Tyr Leu Gly Gly Gly Gly Thr Arg Gly Asn Gly Arg Ile 
            180                 185                 190         


Gln Phe Lys Asp Thr Asn Ile Glu Thr Val Val Gly Glu Tyr Asp Ser 
        195                 200                 205             


Thr Asn Leu Lys Ile Lys 
    210                 


<210>  5
<211>  304
<212>  PRT
<213>  artificial sequence

<220>
<223>  CRISPR-associated enzyme

<400>  5

Met Thr Leu Ala Thr Lys Val Phe Lys Leu Ser Phe Lys Thr Pro Val 
1               5                   10                  15      


His Phe Gly Lys Lys Arg Leu Ser Asp Gly Glu Met Thr Ile Thr Ala 
            20                  25                  30          


Asp Thr Leu Phe Ser Ala Leu Phe Ile Glu Thr Leu Gln Leu Gly Lys 
        35                  40                  45              


Asp Thr Asp Trp Leu Leu Asn Asp Leu Ile Ile Ser Asp Thr Phe Pro 
    50                  55                  60                  


Tyr Glu Asn Glu Leu Tyr Tyr Leu Pro Lys Pro Leu Ile Lys Ile Asp 
65                  70                  75                  80  


Ser Lys Glu Glu Asp Asn His Lys Ala Phe Lys Lys Leu Lys Tyr Val 
                85                  90                  95      


Pro Val His His Tyr Asn Gln Tyr Leu Asn Gly Glu Leu Ser Ala Glu 
            100                 105                 110         


Asp Ala Thr Asp Leu Asn Asp Ile Phe Asn Ile Gly Tyr Phe Ser Leu 
        115                 120                 125             


Gln Thr Lys Val Ser Leu Ile Ala Gln Glu Thr Asp Ser Ser Ala Asp 
    130                 135                 140                 


Ser Glu Pro Tyr Ser Val Gly Thr Phe Thr Phe Glu Pro Glu Ala Gly 
145                 150                 155                 160 


Leu Tyr Phe Ile Ala Lys Gly Ser Glu Glu Thr Leu Asp His Leu Asn 
                165                 170                 175     


Asn Ile Met Thr Ala Leu Gln Tyr Ser Gly Leu Gly Gly Lys Arg Asn 
            180                 185                 190         


Ala Gly Tyr Gly Gln Phe Glu Tyr Glu Ile Ile Asn Asn Gln Gln Leu 
        195                 200                 205             


Ser Lys Leu Leu Asn Gln Asn Gly Lys His Ser Ile Leu Leu Ser Thr 
    210                 215                 220                 


Ala Met Ala Lys Lys Glu Glu Ile Glu Ser Ala Leu Lys Glu Ala Arg 
225                 230                 235                 240 


Tyr Ile Leu Thr Lys Arg Ser Gly Phe Val Gln Ser Thr Asn Tyr Ser 
                245                 250                 255     


Glu Met Leu Val Lys Lys Ser Asp Phe Tyr Ser Phe Ser Ser Gly Ser 
            260                 265                 270         


Val Phe Lys Asn Ile Phe Asn Gly Asp Ile Phe Asn Val Gly His Asn 
        275                 280                 285             


Gly Lys His Pro Val Tyr Arg Tyr Ala Lys Pro Leu Trp Leu Glu Val 
    290                 295                 300                 


<210>  6
<211>  340
<212>  PRT
<213>  artificial sequence

<220>
<223>  CRISPR-associated enzyme

<400>  6

Met Thr Ile Lys Asn Tyr Glu Val Val Ile Lys Thr Leu Gly Pro Ile 
1               5                   10                  15      


His Ile Gly Ser Gly Gln Val Met Lys Lys Gln Asp Tyr Ile Tyr Asp 
            20                  25                  30          


Phe Tyr Asn Ser Lys Val Tyr Met Ile Asn Gly Asn Lys Leu Val Lys 
        35                  40                  45              


Phe Leu Lys Arg Lys Asn Leu Leu Tyr Thr Tyr Gln Asn Phe Leu Arg 
    50                  55                  60                  


Tyr Pro Pro Lys Asn Pro Arg Glu Asn Gly Leu Lys Asp Tyr Leu Asp 
65                  70                  75                  80  


Ala Gln Asn Val Lys Gln Ser Glu Trp Glu Ala Phe Val Ser Tyr Ser 
                85                  90                  95      


Glu Lys Val Asn Gln Gly Lys Lys Tyr Gly Asn Thr Arg Pro Lys Pro 
            100                 105                 110         


Leu Asn Asp Leu His Leu Met Val Arg Asp Gly Gln Asn Lys Val Tyr 
        115                 120                 125             


Leu Pro Gly Ser Ser Ile Lys Gly Ala Ile Lys Thr Thr Leu Val Ser 
    130                 135                 140                 


Lys Tyr Asn Asn Glu Lys Asn Lys Asp Ile Tyr Ser Lys Ile Lys Val 
145                 150                 155                 160 


Ser Asp Ser Lys Pro Ile Asp Glu Ser Asn Leu Ala Ile Tyr Gln Lys 
                165                 170                 175     


Ile Asp Ile Asn Lys Ser Glu Lys Ser Met Pro Leu Tyr Arg Glu Cys 
            180                 185                 190         


Ile Asp Val Asn Thr Glu Ile Lys Phe Lys Leu Thr Ile Glu Asp Glu 
        195                 200                 205             


Ile Tyr Ser Ile Asn Glu Ile Glu Gln Ser Ile Gln Asp Phe Tyr Lys 
    210                 215                 220                 


Asn Tyr Tyr Asp Lys Trp Leu Val Gly Phe Lys Glu Thr Lys Gly Gly 
225                 230                 235                 240 


Arg Arg Phe Ala Leu Glu Gly Gly Ile Pro Asp Val Leu Asn Gln Asn 
                245                 250                 255     


Ile Leu Phe Leu Gly Ala Gly Thr Gly Phe Val Ser Lys Thr Thr His 
            260                 265                 270         


Tyr Gln Leu Lys Asn Arg Lys Gln Ala Lys Gln Asp Ser Phe Glu Ile 
        275                 280                 285             


Leu Thr Lys Lys Phe Arg Gly Thr Tyr Gly Lys Met Lys Glu Ile Pro 
    290                 295                 300                 


Ser Asn Val Pro Val Ala Leu Lys Gly Thr Thr Asn Gln Ser Arg His 
305                 310                 315                 320 


Thr Ser Tyr Gln Gln Gly Met Cys Lys Val Ser Phe Gln Glu Leu Asn 
                325                 330                 335     


Asn Glu Val Leu 
            340 


<210>  7
<211>  422
<212>  PRT
<213>  artificial sequence

<220>
<223>  CRISPR-associated enzyme

<400>  7

Met Lys Ile Leu Phe Ser Pro Ile Gly Asn Ser Asp Pro Trp Arg Asn 
1               5                   10                  15      


Asp Arg Asp Gly Ala Met Leu His Ile Val Arg His Tyr Asn Leu Asp 
            20                  25                  30          


Lys Val Val Leu Tyr Phe Thr Arg Thr Ile Trp Glu Gly Asn Glu Asn 
        35                  40                  45              


Arg Lys Gly His Lys Ile Tyr Glu Trp Glu Lys Ile Ile Gln Thr Val 
    50                  55                  60                  


Ser Pro Asn Thr Glu Val Glu Ile Ile Ile Glu Asn Val Asp Asn Ala 
65                  70                  75                  80  


Gln Asp Tyr Asp Val Phe Lys Glu Lys Phe His Lys Tyr Leu Lys Ile 
                85                  90                  95      


Ile Glu Asp Ser Tyr Glu Asp Cys Glu Ile Ile Leu Asn Val Thr Ser 
            100                 105                 110         


Gly Thr Pro Gln Met Glu Ser Thr Leu Cys Leu Glu Tyr Ile Val Tyr 
        115                 120                 125             


Pro Glu Asn Lys Lys Cys Val Gln Val Ser Thr Pro Thr Lys Asp Ser 
    130                 135                 140                 


Asn Ala Gly Ile Glu Tyr Ser Asn Pro Lys Asp Lys Val Glu Glu Phe 
145                 150                 155                 160 


Glu Ile Val Asn Glu Val Glu Lys Lys Ser Glu Lys Arg Cys Lys Glu 
                165                 170                 175     


Ile Asn Ile Leu Ser Phe Arg Glu Ala Met Ile Arg Ser Gln Ile Leu 
            180                 185                 190         


Gly Leu Ile Asp Asn Tyr Asp Tyr Glu Gly Ala Leu Asn Leu Val Ser 
        195                 200                 205             


Asn Gln Lys Ser Phe Arg Asn Gly Lys Leu Leu Arg Lys Lys Leu Leu 
    210                 215                 220                 


Ser Leu Thr Lys Gln Ile Lys Thr His Glu Val Phe Pro Glu Ile Asn 
225                 230                 235                 240 


Glu Lys Tyr Arg Asp Asp Ala Leu Lys Lys Ser Leu Phe His Tyr Leu 
                245                 250                 255     


Leu Leu Asn Met Arg Tyr Asn Arg Leu Asp Val Ala Glu Thr Leu Ile 
            260                 265                 270         


Arg Val Lys Ser Ile Ala Glu Phe Ile Leu Lys Thr Tyr Ile Glu Ile 
        275                 280                 285             


His Trp Pro Thr Leu Ile Ile Glu Lys Asp Gly Lys Pro Tyr Leu Asn 
    290                 295                 300                 


Asp Glu Asp Asn Leu Ser Phe Val Tyr Lys Tyr Asn Leu Leu Leu Glu 
305                 310                 315                 320 


Lys Arg Lys Gln Asn Phe Asp Val Ser Arg Ile Leu Gly Leu Pro Ala 
                325                 330                 335     


Phe Ile Asp Ile Leu Thr Ile Leu Glu Pro Asn Ser Gln Leu Leu Lys 
            340                 345                 350         


Glu Val Asn Ala Val Asn Asp Ile Asn Gly Leu Arg Asn Ser Ile Ala 
        355                 360                 365             


His Asn Leu Asp Thr Leu Asn Leu Asp Lys Asn Lys Asn Tyr Lys Lys 
    370                 375                 380                 


Ile Met Leu Ser Val Glu Ala Ile Lys Asn Met Leu His Ile Ser Phe 
385                 390                 395                 400 


Pro Glu Ile Glu Glu Glu Asp Tyr Asn Tyr Phe Glu Glu Lys Asn Lys 
                405                 410                 415     


Glu Phe Lys Glu Leu Leu 
            420         


<210>  8
<211>  244
<212>  PRT
<213>  artificial sequence

<220>
<223>  CRISPR-associated enzyme

<400>  8

Met Ile Asn Lys Ile Thr Val Glu Leu Asp Leu Pro Glu Ser Ile Arg 
1               5                   10                  15      


Phe Gln Tyr Leu Gly Ser Val Leu His Gly Val Leu Met Asp Tyr Leu 
            20                  25                  30          


Ser Asp Asp Ile Ala Asp Gln Leu His His Glu Phe Ala Tyr Ser Pro 
        35                  40                  45              


Leu Lys Gln Arg Ile Tyr His Lys Asn Lys Lys Ile Ile Trp Glu Ile 
    50                  55                  60                  


Val Cys Met Ser Asp Asn Leu Phe Lys Glu Val Val Lys Leu Phe Ser 
65                  70                  75                  80  


Ser Lys Asn Ser Leu Leu Leu Lys Tyr Tyr Gln Thr Asn Ile Asp Ile 
                85                  90                  95      


Gln Ser Phe Gln Ile Glu Lys Ile Asn Val Gln Asn Met Met Asn Gln 
            100                 105                 110         


Leu Leu Gln Val Glu Asp Leu Ser Arg Tyr Val Arg Leu Asn Ile Gln 
        115                 120                 125             


Thr Pro Met Ser Phe Lys Tyr Gln Asn Ser Tyr Met Ile Phe Pro Asp 
    130                 135                 140                 


Val Lys Arg Phe Phe Arg Ser Ile Met Ile Gln Phe Asp Ala Phe Phe 
145                 150                 155                 160 


Glu Glu Tyr Arg Met Tyr Asp Lys Glu Thr Leu Asn Phe Leu Glu Lys 
                165                 170                 175     


Asn Val Asn Ile Val Asp Tyr Lys Leu Lys Ser Thr Arg Phe Asn Leu 
            180                 185                 190         


Glu Lys Val Lys Ile Pro Ser Phe Thr Gly Glu Ile Val Phe Lys Ile 
        195                 200                 205             


Lys Gly Pro Leu Pro Phe Leu Gln Leu Thr His Phe Leu Leu Lys Phe 
    210                 215                 220                 


Gly Glu Phe Ser Gly Ser Gly Ile Lys Thr Ser Leu Gly Met Gly Lys 
225                 230                 235                 240 


Tyr Ser Ile Ile 
                


<210>  9
<211>  469
<212>  DNA
<213>  artificial sequence

<220>
<223>  CRISPR locus

<400>  9
cgaaatataa aaagaaatga aaggttaaat taatattaat tttattaaat gaataggcta       60

aaccatctta aatgtagtat actattaata taaatgtaat tattataaaa tttgtcaaaa      120

aaagtgacat atcatataat cttgtactag tgattgtcat attttttgac agcaaaaatg      180

atgcttgaaa tatagttgtg atggcatttg ttaaagtatc ggatcgatac ccaccccgaa      240

gaaaagggga cgagaacacg tatgccgaag tatataaatc atcagtacaa aggatcgata      300

cccaccccga agaaaagggg acgagaacta gtaataattg tcatttgcat acgttacatc      360

gatgatcgat acccaccccg aagaaaaggg gacgagaact agtacggtcg tgaacatttt      420

ttcttgattc tctgatcgat agccaccccg aagaaaaggg ggcagagtg                  469


<210>  10
<211>  35
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide

<400>  10
aatttttaat ttaagttctt gttcatcgtc ataaa                                  35


<210>  11
<211>  35
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide

<400>  11
tatttatgac gatgaacaag aacttaaatt aaaaa                                  35


<210>  12
<211>  35
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide

<400>  12
aatttcgagg aagttgcaat tgataatgaa aaatt                                  35


<210>  13
<211>  35
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide

<400>  13
aatttttcat tatcaattgc aacttcctcg aaatt                                  35


<210>  14
<211>  30
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide

<400>  14
gaaatttcca gcagaaactt taccgaaata                                        30


<210>  15
<211>  34
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide

<400>  15
cattttgttt ctgttcatgc ctctgccgac tgct                                   34


<210>  16
<211>  34
<212>  DNA
<213>  artificial sequence

<220>
<223>  oligonucleotide

<400>  16
agcagtcggc agaggcatga acagaaacaa aatg                                   34


<210>  17
<211>  36
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  17
taataagttt tatgctcctc agtttttaaa tcactt                                 36


<210>  18
<211>  36
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  18
aagtgattta aaaactgagg agcataaaac ttatta                                 36


<210>  19
<211>  35
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  19
tttttaaaaa ttctttggtt accatgcatc tcgct                                  35


<210>  20
<211>  35
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  20
agcgagatgc atggtaacca aagaattttt aaaaa                                  35


<210>  21
<211>  36
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  21
ttaaatcttt gattgctctt agctctagtt atgtat                                 36


<210>  22
<211>  36
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  22
gtaaaccttt gattgctctt agctcgagtt atgtgc                                 36


<210>  23
<211>  36
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  23
atacataact agagctaaga gcaatcaaag atttaa                                 36


<210>  24
<211>  36
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  24
attcgtcatc ttcaagtaat gcctctaaat caataa                                 36


<210>  25
<211>  36
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  25
ttattgattt agaggcatta cttgaagatg acgaat                                 36


<210>  26
<211>  30
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  26
acttcacaca agataacatt attgatttag                                        30


<210>  27
<211>  35
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  27
gcatgcacct tgcctgaatg ttttaaaaat tcatt                                  35


<210>  28
<211>  35
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  28
aatgaatttt taaaacattc aggcaaggtg catgc                                  35


<210>  29
<211>  35
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  29
atgtcaccta agtcaacacc atcatttttt attct                                  35


<210>  30
<211>  35
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  30
cttaggtgac attggctgtc gattttacac tgaag                                  35


<210>  31
<211>  35
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  31
ttatgatttt ttggagcata taaatcattt agtgt                                  35


<210>  32
<211>  35
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  32
cagaaagtgt attgcaacag attggctcaa aagtt                                  35


<210>  33
<211>  34
<212>  DNA
<213>  artificial seq

<220>
<223>  spacer sequence

<400>  33
ataaaagaaa agggattgat aaactatgaa atta                                   34


<210>  34
<211>  36
<212>  DNA
<213>  artificial seq

<220>
<223>  spacer sequence

<400>  34
tatgtattga tctcgattct cgttagtttc taaatt                                 36


<210>  35
<211>  36
<212>  DNA
<213>  artificial seq

<220>
<223>  spacer sequence

<400>  35
catttggaaa ctaacgagaa tcgagctcaa tacacg                                 36


<210>  36
<211>  38
<212>  DNA
<213>  artificial sequence

<220>
<223>  spacer sequence

<400>  36
cacgctgtag tgaagtatag aaacggcatg agtacaat                               38


<210>  37
<211>  38
<212>  DNA
<213>  artificial sequence

<220>
<223>  spacer sequence

<400>  37
attgtactca tgccgtttct atacttcact acagcgtg                               38


<210>  38
<211>  35
<212>  DNA
<213>  artificial seq

<220>
<223>  spacer sequence

<400>  38
acgtatgccg aagtatataa atcatcagta caaag                                  35


<210>  39
<211>  35
<212>  DNA
<213>  artificial sequence

<220>
<223>  spacer sequence

<400>  39
ctttgtactg atgatttata tacttcggca tacgt                                  35


<210>  40
<211>  35
<212>  DNA
<213>  artificial seq

<220>
<223>  spacer sequence

<400>  40
tagtaataat tgtcatttgc atacgttaca tcgat                                  35


<210>  41
<211>  35
<212>  DNA
<213>  artificial seq

<220>
<223>  spacer sequence

<400>  41
atcgatgtaa catatgcaaa tgacaattat tacta                                  35


<210>  42
<211>  34
<212>  DNA
<213>  artificial seq

<220>
<223>  spacer sequence

<400>  42
tagaatgtta ttatctaagt ggtcgatgta ttcc                                   34


<210>  43
<211>  34
<212>  DNA
<213>  artificial seq

<220>
<223>  spacer sequence

<400>  43
ggaatacatc gaccacatag ataataacat ccta                                   34


<210>  44
<211>  35
<212>  DNA
<213>  artificial seq

<220>
<223>  spacer sequence

<400>  44
aagttaacgg cattacctaa taaaaatatt ttagg                                  35


<210>  45
<211>  35
<212>  DNA
<213>  artificial seq

<220>
<223>  spacer sequence

<400>  45
cctaaaatgt ttttattagg taatgcggtc aactt                                  35


<210>  46
<211>  33
<212>  DNA
<213>  artificial sequence

<220>
<223>  spacer sequence

<400>  46
tcatctttca tgtcactgat taattcattt gta                                    33


<210>  47
<211>  33
<212>  DNA
<213>  artificial seq

<220>
<223>  spacer sequence

<400>  47
tacaaatgaa ttaatcaatg atatgaaaga tga                                    33


<210>  48
<211>  35
<212>  DNA
<213>  artificial seq

<220>
<223>  spacer sequence

<400>  48
ccaaaccatt tagcacgata tttattaaaa ccata                                  35


<210>  49
<211>  35
<212>  DNA
<213>  artificial seq

<220>
<223>  spacer sequence

<400>  49
tacggattta ataaatatcg tgccaaatgg tttgg                                  35


<210>  50
<211>  35
<212>  DNA
<213>  artificial seq

<220>
<223>  spacer sequence

<400>  50
tatttttctc ctttagcaat cattctgtct agtac                                  35


<210>  51
<211>  35
<212>  DNA
<213>  artificial seq

<220>
<223>  spacer sequence

<400>  51
gtattagaca gaatgattgc tagaggagaa caata                                  35


<210>  52
<211>  34
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  52
ctttgtactg atgatttata tacttcggca tacg                                   34


<210>  53
<211>  20
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucloetide

<400>  53
taaatctaac aacactctaa                                                   20


<210>  54
<211>  33
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  54
aaaggtacca aatttaatgc tattttcctt cgc                                    33


<210>  55
<211>  31
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  55
aaaagatcta ataatgtatt tacgctgggg c                                      31


<210>  56
<211>  62
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  56
gttctcgtcc ccttttcttc ggggtgggta tcgatccttt gtactgatga tttatatact       60

tc                                                                      62


<210>  57
<211>  61
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  57
agcagtcggc agaggcatga acagaaacaa aatgtaaatc taacaacact ctaaaaaatt       60

g                                                                       61


<210>  58
<211>  62
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  58
tatttatgac gatgaacaag aacttaaatt aaaaataaat ctaacaacac tctaaaaaat       60

tg                                                                      62


<210>  59
<211>  62
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  59
agcgagatgc atggtaacca aagaattttt aaaaataaat ctaacaacac tctaaaaaat       60

tg                                                                      62


<210>  60
<211>  62
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  60
aatgaatttt taaaacattc aggcaaggtg catgctaaat ctaacaacac tctaaaaaat       60

tg                                                                      62


<210>  61
<211>  63
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  61
ttaaatcttt gattgctctt agctctagtt atgtattaaa tctaacaaca ctctaaaaaa       60

ttg                                                                     63


<210>  62
<211>  63
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  62
gtaaaccttt gattgctctt agctcgagtt atgtgctaaa tctaacaaca ctctaaaaaa       60

ttg                                                                     63


<210>  63
<211>  62
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  63
cttaggtgac attggctgtc gattttacac tgaagtaaat ctaacaacac tctaaaaaat       60

tg                                                                      62


<210>  64
<211>  62
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  64
cagaaagtgt attgcaacag attggctcaa aagtttaaat ctaacaacac tctaaaaaat       60

tg                                                                      62


<210>  65
<211>  62
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  65
atgtcaccta agtcaacacc atcatttttt attcttaaat ctaacaacac tctaaaaaat       60

tg                                                                      62


<210>  66
<211>  62
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  66
ttatgatttt ttggagcata taaatcattt agtgttaaat ctaacaacac tctaaaaaat       60

tg                                                                      62


<210>  67
<211>  61
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  67
cattttgttt ctgttcatgc ctctgccgac tgcttaaatc taacaacact ctaaaaaatt       60

g                                                                       61


<210>  68
<211>  62
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  68
aatttttcat tatcaattgc aacttcctcg aaatttaaat ctaacaacac tctaaaaaat       60

tg                                                                      62


<210>  69
<211>  62
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  69
aatttcgagg aagttgcaat tgataatgaa aaatttaaat ctaacaacac tctaaaaaat       60

tg                                                                      62


<210>  70
<211>  63
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  70
atacataact agagctaaga gcaatcaaag atttaataaa tctaacaaca ctctaaaaaa       60

ttg                                                                     63


<210>  71
<211>  63
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  71
attcgtcatc ttcaagtaat gcctctaaat caataataaa tctaacaaca ctctaaaaaa       60

ttg                                                                     63


<210>  72
<211>  58
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  72
tgagaccagt ctcggaagct caaaggtctc ttaaatctaa caacactcta aaaaattg         58


<210>  73
<211>  40
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  73
gaacttattg atttagaggc attacttgaa gatgacgaat                             40


<210>  74
<211>  40
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  74
tttaattcgt catcttcaag taatgcctct aaatcaataa                             40


<210>  75
<211>  39
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  75
gaacaatttt taatttaagt tcttgttcat cgtcataaa                              39


<210>  76
<211>  39
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  76
tttatttatg acgatgaaca agaacttaaa ttaaaaatt                              39


<210>  77
<211>  39
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  77
gaacttttta aaaattcttt ggttaccatg catctcgct                              39


<210>  78
<211>  39
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  78
tttaagcgag atgcatggta accaaagaat ttttaaaaa                              39


<210>  79
<211>  39
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  79
gaacgcatgc accttgcctg aatgttttaa aaattcatt                              39


<210>  80
<211>  39
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  80
tttaaatgaa tttttaaaac attcaggcaa ggtgcatgc                              39


<210>  81
<211>  40
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  81
gaacaagtga tttaaaaact gaggagcata aaacttatta                             40


<210>  82
<211>  40
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  82
tttataataa gttttatgct cctcagtttt taaatcactt                             40


<210>  83
<211>  40
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  83
gaactaataa gttttatgct cctcagtttt taaatcactt                             40


<210>  84
<211>  40
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  84
tttaaagtga tttaaaaact gaggagcata aaacttatta                             40


<210>  85
<211>  32
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  85
aaactcgtgg attctgtgat ttggatcctt cc                                     32


<210>  86
<211>  32
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  86
aaaaagatct tatgactgtt atgtggttat cg                                     32


<210>  87
<211>  31
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  87
aaaaagatct tgcataattc acgctgacct c                                      31


<210>  88
<211>  32
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  88
aaaacacgag cgtttgttga actaatgggt gc                                     32


<210>  89
<211>  53
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  89
aaaaagcgca agaagaaatc aaccagcgca ctcgtagact atttttgtct aaa              53


<210>  90
<211>  44
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  90
acactgagac ttgttgagtt caaacgaaaa ttggataaag tggg                        44


<210>  91
<211>  27
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  91
attatttctt aataactaaa aatatgg                                           27


<210>  92
<211>  44
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  92
ctttatccaa ttttcgtttg aactcaacaa gtctcagtgt gctg                        44


<210>  93
<211>  35
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  93
aaacacttca cacaagataa cattattgat ttagg                                  35


<210>  94
<211>  35
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  94
aaaacctaaa tcaataatgt tatcttgtgt gaagt                                  35


<210>  95
<211>  35
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  95
aaacgaaatt tccagcagaa actttaccga aatag                                  35


<210>  96
<211>  35
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucletide

<400>  96
aaaactattt cggtaaagtt tctgctggaa atttc                                  35


<210>  97
<211>  21
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  97
gaagctttag ctttgcagtg g                                                 21


<210>  98
<211>  22
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  98
ctgtaataga catcgttcgc ag                                                22


<210>  99
<211>  26
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  99
gctacattaa ttatagggaa tcttac                                            26


<210>  100
<211>  23
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  100
tcctaacaga aattgcgtta aag                                               23


<210>  101
<211>  26
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  101
ttttatacaa tactatttat aagtgc                                            26


<210>  102
<211>  24
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  102
catatagttt tatgcctaaa aacc                                              24


<210>  103
<211>  25
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  103
atatatttat ttggctcata tttgc                                             25


<210>  104
<211>  28
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  104
aaactcgagc gcgcaagctg gggatccg                                          28


<210>  105
<211>  33
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  105
aaactcgagt aggtactaaa acaattcatc cag                                    33


<210>  106
<211>  30
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  106
aaactcgagc tgagagtgca ccatatgcgg                                        30


<210>  107
<211>  31
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  107
aaactcgagc ttaatagctc acgctatgcc g                                      31


<210>  108
<211>  46
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  108
taattcctcc tttttgttga cattatatca ttgatagagt tatttg                      46


<210>  109
<211>  46
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  109
actctatcaa tgatataatg tcaacaaaaa ggaggaatta atgatg                      46


<210>  110
<211>  46
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  110
tgacactcta tcattgatag agcataatta aaataagctt gatatc                      46


<210>  111
<211>  46
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  111
aagcttattt taattatgct ctatcaatga tagagtgtca atattt                      46


<210>  112
<211>  33
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  112
ttgatagagt gatatcgaat tcggaggcat atc                                    33


<210>  113
<211>  32
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  113
tgatagagag cttattttaa ttatgctcta tc                                     32


<210>  114
<211>  65
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  114
gatctcaaga taacattatt gatttagagg cattacttga agatgacgaa ttagaagcaa       60

accgc                                                                   65


<210>  115
<211>  59
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  115
ggtttgcttc taattcgtca tcttcaagta atgcctctaa atcaataatg ttatcttga        59


<210>  116
<211>  65
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  116
gatcttttgc ttctaattcg tcatcttcaa gtaatgcctc taaatcaata atgttatctt       60

gccgc                                                                   65


<210>  117
<211>  59
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  117
ggcaagataa cattattgat ttagaggcat tacttgaaga tgacgaatta gaagcaaaa        59


<210>  118
<211>  24
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  118
tcttattcaa gacaacactt acac                                              24


<210>  119
<211>  23
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  119
atctaacatc tcaatggcta agg                                               23


<210>  120
<211>  47
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  120
ccacatacct atatctgccc tttttctgcc cttttttatt tttaaag                     47


<210>  121
<211>  39
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  121
gtgtactaaa aggtaatcga tacggttata tttattccc                              39


<210>  122
<211>  41
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  122
ggcagaaaaa gggcagatat aggtatgtgg ttttgtattg g                           41


<210>  123
<211>  39
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  123
tataaccgta tcgattacct tttagtacac aagtttttc                              39


<210>  124
<211>  23
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  124
gttaatgtta cgaatgatga acc                                               23


<210>  125
<211>  22
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  125
ttggcaagtt ctgcaccttt ac                                                22


<210>  126
<211>  23
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  126
aagatgcaac aatgggaacc aag                                               23


<210>  127
<211>  27
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  127
ctaaatgtga tataataaaa taaaaag                                           27


<210>  128
<211>  23
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  128
ataaagacac cgattcaact atg                                               23


<210>  129
<211>  24
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  129
aagataaaga atttgctcaa gacg                                              24


<210>  130
<211>  23
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  130
ttcatcagct gacattaact cac                                               23


<210>  131
<211>  23
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  131
gcaagagagt taaaaggtat acg                                               23


<210>  132
<211>  24
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  132
ctgtatatcc ttgtatcaac tatc                                              24


<210>  133
<211>  23
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  133
cctatctgac aattcctgaa tag                                               23


<210>  134
<211>  21
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  134
taccctagtt aacgtctctt g                                                 21


<210>  135
<211>  23
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  135
cgtttcggta cttatttcaa cac                                               23


<210>  136
<211>  25
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  136
gttaattcta tgtccatttg taacc                                             25


<210>  137
<211>  55
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  137
ggggacaagt ttgtacaaaa aagcaggcta ttcgaaattg tacctgtttc atctc            55


<210>  138
<211>  46
<212>  DNA
<213>  artificial organism

<220>
<223>  oligonucleotide

<400>  138
cgaaaaaaga gtgtcttgtg atggtatcat atcggtatca aataac                      46


<210>  139
<211>  44
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  139
tgataccgat atgataccat cacaagacac tcttttttcg cacc                        44


<210>  140
<211>  46
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  140
ctatgaacat atttgattaa cgtatataga tttcataaag tctaac                      46


<210>  141
<211>  47
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  141
ctttatgaaa tctatatacg ttaatcaaat atgttcatag cttgatg                     47


<210>  142
<211>  53
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  142
ggggaccact ttgtacaaga aagctgggtc attagatata aagatgtata cgg              53


<210>  143
<211>  18
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  143
tacgactcac tatagggg                                                     18


<210>  144
<211>  23
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  144
tctacttaat ctgataagtg agc                                               23


<210>  145
<211>  28
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  145
aaacccggga cgcaaaccgc ctctcccc                                          28


<210>  146
<211>  31
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  146
cctccttata aaattagtat aattatagca c                                      31


<210>  147
<211>  20
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  147
gtgacctcct tgccattgtc                                                   20


<210>  148
<211>  44
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  148
tatacttcgg catacgtgtt ctcgttatct tgttcatatt tatc                        44


<210>  149
<211>  44
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  149
gataaatatg aacaagataa cgagaacacg tatgccgaag tata                        44


<210>  150
<211>  58
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  150
tgagaccagt ctcggaagct caaaggtctc ttaaatctaa caacactcta aaaaattg         58


<210>  151
<211>  58
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  151
gttctcgtcc ccttttcttc ggggtgggta tcgatccgat actttaacaa atgccatc         58


<210>  152
<211>  39
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  152
gaacattcgt catcttcaag taatgcctct aaatcaata                              39


<210>  153
<211>  39
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  153
gatctattga tttagaggca ttacttgaag atgacgaat                              39


<210>  154
<211>  50
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  154
ggtaaatcaa aactaactaa caaatacatt agtttcccac ctctatcatc                  50


<210>  155
<211>  50
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  155
gatgatagag gtgggaaact aatgtatttg ttagttagtt ttgatttacc                  50


<210>  156
<211>  55
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  156
tatttagaga acgtatgccg aagtatataa atcatcagta caaaggtaag aatca            55


<210>  157
<211>  55
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  157
tgattcttac ctttgtactg atgatttata tacttcggca tacgttctct aaata            55


<210>  158
<211>  90
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  158
gcggtaattt taatgagata tttagagaac gtatgccgaa gtatataaat catcagtaca       60

aaggtaagaa tcacagtaaa cagcgcgcgg                                        90


<210>  159
<211>  90
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  159
ccgcgcgctg tttactgtga ttcttacctt tgtactgatg atttatatac ttcggcatac       60

gttctctaaa tatctcatta aaattaccgc                                        90


<210>  160
<211>  58
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  160
gcggcgtaga gaacgtatgc cgaagtatat aaatcatcag tacaaaggta aggcggcg         58


<210>  161
<211>  58
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  161
cgccgcgaat ggccctttga ctaccccccc ccccaagccg ccctttagag atcgccgc         58


<210>  162
<211>  58
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  162
gcgcggctta cctttgtact gatgatttat atacttcggc atacgttctc tagcggcg         58


<210>  163
<211>  58
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  163
cgccgcatct cttttccccg gcttcccccc ccccgtagtc tttcccccat tcccgcgc         58


<210>  164
<211>  54
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  164
gaatctagta tgattggagc aattgcttct cctgtagtta gagatttgca aacc             54


<210>  165
<211>  54
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  165
ggtttgcaaa tctctaacta caggagaagc aattgctcca atcatactag attc             54


<210>  166
<211>  90
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  166
gcggtaattt taatgagata acgagaacac gtatgccgaa gtatataaat catcagtaca       60

aaggtaagaa tcacagtaaa cagcgcgcgg                                        90


<210>  167
<211>  90
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  167
ccgcgcgctg tttactgtga ttcttacctt tgtactgatg atttatatac ttcggcatac       60

gtgttctcgt tatctcatta aaattaccgc                                        90


<210>  168
<211>  47
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  168
ctgctatata ttcaggcggt gccgctttat ttttaatcgg tgcatgg                     47


<210>  169
<211>  47
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  169
ccatgcaccg attaaaaata aagcggcacc gcctgaatat atagcag                     47


<210>  170
<211>  38
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  170
ggcgaatttt caggttcagg tataaaaaca agcttagg                               38


<210>  171
<211>  38
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  171
cctaagcttg tttttatacc tgaacctgaa aattcgcc                               38


<210>  172
<211>  25
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  172
tgataaatat aatactctaa cgctg                                             25


<210>  173
<211>  25
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  173
acagcgttag agtattatat ttatc                                             25


<210>  174
<211>  25
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  174
aataacatct ttcatttttc catcc                                             25


<210>  175
<211>  24
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  175
gggatggaaa aatgaaagat gtta                                              24


<210>  176
<211>  43
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  176
cttcggggtg ggtatcgatc agagaccttt gagcttccga gac                         43


<210>  177
<211>  43
<212>  DNA
<213>  artificial seq

<220>
<223>  oligonucleotide

<400>  177
aaaaggggac gagaactaaa tctaacaaca ctctaaaaaa ttg                         43


<210>  178
<211>  55
<212>  RNA
<213>  artificial seq

<220>
<223>  RNA oligonucleotide

<400>  178
ugauucuuac cuuuguacug augauuuaua uacuucggca uacguucucu aaaua            55


<210>  179
<211>  55
<212>  RNA
<213>  artificial seq

<220>
<223>  RNA oligonucleotide

<400>  179
gcuguuaagu uacucgagca caucagugau agccuuauuc ccgcugugcc uauac            55


<210>  180
<211>  55
<212>  RNA
<213>  artificial seq

<220>
<223>  RNA oligonucleotide

<400>  180
ugauucuuac cuuuguacug augauuuaua uacuucggca uacguguucu cguua            55


<210>  181
<211>  10
<212>  RNA
<213>  artificial seq

<220>
<223>  RNA oligonucleotide

<400>  181
guuuacugug                                                              10


<210>  182
<211>  10
<212>  RNA
<213>  artificial seq

<220>
<223>  RNA oligonucleotide

<400>  182
uuaaugagau                                                              10


<210>  183
<211>  34
<212>  DNA
<213>  artificial seq

<220>
<223>  spacer sequence

<400>  183
gtttttcata gttaatcaat cccttttctt tttt                                   34


<210>  184
<211>  44
<212>  RNA
<213>  artificial sequence

<220>
<223>  crRNA

<400>  184
acgagaacuu aaaucuuuga uugcucuuag cucuaguuau guau                        44


<210>  185
<211>  60
<212>  DNA
<213>  artificial sequence

<220>
<223>  Target

<400>  185
aaacatacgc acataactcg agctaagagc aatcaaaggt ttacagttgt tgaggcagag       60


<210>  186
<211>  60
<212>  DNA
<213>  artificial sequence

<220>
<223>  Target

<400>  186
ctctgcctca acaactgtaa acctttgatt gctcttagct cgagttatgt gcgtatgttt       60


<210>  187
<211>  45
<212>  DNA
<213>  artificial sequence

<220>
<223>  Promoter

<400>  187
tacactttta ttatactatg aaaaatcgta attgcaaccc ttaaa                       45


<210>  188
<211>  45
<212>  DNA
<213>  artificial sequence

<220>
<223>  Promoter

<400>  188
tttaagggtt gcaattacga tttttcatag tataataaaa gtgta                       45


<210>  189
<211>  41
<212>  RNA
<213>  artificial sequence

<220>
<223>  spc1 crRNA

<400>  189
acgagaacac guaugccgaa guauauaaau caucaguaca a                           41


<210>  190
<211>  60
<212>  DNA
<213>  artificial sequence

<220>
<223>  gp43 target region (coding strand sequence)

<400>  190
gtgacttcac acaagataac attattgatt tagaggcatt acttgaagat gacgaattag       60


<210>  191
<211>  45
<212>  DNA
<213>  artificial sequence

<220>
<223>  nes target bottom strand

<400>  191
tttagagaac gtatgccgaa gtatataaat catcagtaca aaggt                       45


<210>  192
<211>  45
<212>  DNA
<213>  artificial sequence

<220>
<223>  target

<400>  192
acctttgtac tgatgattta tatacttcgg catacgttct ctaaa                       45


