                         SEQUENCE LISTING

<110>  MEMORIAL SLOAN KETTERING CANCER CENTER
       ROTOLO, Jimmy
       KOLESNICK, Richard
 
<120>  ANTI-CERAMIDE ANTIBODIES

<130>  32896-226065PCT

<150>  US 62/034,453
<151>  2014-08-07

<160>  45    

<170>  PatentIn version 3.5

<210>  1
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: heavy chain variable region CDR1 of the anti-ceramide 
       antibody

<400>  1

Gly Tyr Thr Phe Thr Asp His Thr Ile His 
1               5                   10  


<210>  2
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: heavy chain variable region CDR2

<400>  2

Tyr Asn Tyr Pro Arg Asp Gly Ser Thr Lys Tyr Asn Glu Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  3
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: heavy chain variable region CDR3

<400>  3

Gly Phe Ile Thr Thr Val Val Pro Ser Ala Tyr 
1               5                   10      


<210>  4
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: light chain variable region CDR1

<400>  4

Arg Ala Ser Lys Ser Ile Ser Lys Tyr Leu Ala 
1               5                   10      


<210>  5
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: light chain variable region CDR2

<400>  5

Ser Gly Ser Thr Leu Gln Ser 
1               5           


<210>  6
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: light chain variable region CDR3

<400>  6

Gln Gln His Asn Glu Tyr Pro Trp Thr 
1               5                   


<210>  7
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: heavy chain variable region sequence

<400>  7

Gln Val Gln Leu Gln Gln Ser Asp Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Val Ser Gly Tyr Thr Phe Thr Asp His 
            20                  25                  30          


Thr Ile His Trp Met Lys Gln Arg Pro Glu Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Asn Pro Arg Asp Gly Ser Thr Lys Tyr Asn Glu Lys Phe Gly 
    50                  55                  60                  


Lys Ala Thr Leu Thr Asp Ala Asp Lys Ser Ser Ser Thr Ala Tyr Met 
65                  70                  75                  80  


Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala 
                85                  90                  95      


Lys Gly Phe Ile Thr Thr Val Val Pro Ser Ala Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Leu Val Thr Val Ser Ala 
        115                 


<210>  8
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: light chain variable region sequence

<400>  8

Asp Val Gln Ile Thr Gln Ser Pro Ser Tyr Leu Ala Ala Ser Pro Gly 
1               5                   10                  15      


Glu Thr Ile Thr Ile Asn Cys Arg Ala Ser Lys Ser Ile Ser Lys Tyr 
            20                  25                  30          


Leu Ala Trp Tyr Gln Glu Lys Pro Gly Lys Thr Asn Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Gly Ser Thr Leu Gln Ser Gly Ile Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Met Tyr Tyr Cys Gln Gln His Asn Glu Tyr Pro Trp 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  9
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: heavy chain variable region CDR1

<400>  9

Gly Tyr Ala Phe Ser Ser Tyr Trp Met Asn 
1               5                   10  


<210>  10
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: heavy chain variable region CDR2

<400>  10

Gln Ile Tyr Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  11
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: heavy chain variable region CDR3

<400>  11

Arg Cys Tyr Tyr Gly Leu Tyr Phe Asp Val 
1               5                   10  


<210>  12
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: light chain variable region CDR1

<400>  12

Lys Ala Ser Gln Asp Ile Asn Arg Tyr Leu Ser 
1               5                   10      


<210>  13
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: light chain variable region CDR2

<400>  13

Arg Ala Asn Arg Leu Val Asp 
1               5           


<210>  14
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: light chain variable region CDR3

<400>  14

Leu Gln Tyr Asp Glu Phe Pro Tyr Thr 
1               5                   


<210>  15
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: heavy chain variable region sequence

<400>  15

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ala Phe Ser Ser Tyr 
            20                  25                  30          


Trp Met Asn Trp Val Lys Gln Arg Pro Gly Lys Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Gln Ile Tyr Pro Gly Asp Gly Asp Thr Asn Tyr Asn Gly Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Thr Arg Arg Cys Tyr Tyr Gly Leu Tyr Phe Asp Val Trp Gly Thr Gly 
            100                 105                 110         


Thr Thr Val Thr Val Ser Ser 
        115                 


<210>  16
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: light chain variable region sequence

<400>  16

Asp Ile Lys Met Thr Gln Ser Pro Ser Ser Arg Tyr Ala Ser Leu Gly 
1               5                   10                  15      


Glu Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Ile Asn Arg Tyr 
            20                  25                  30          


Leu Ser Trp Phe Gln Gln Lys Pro Gly Lys Ser Pro Lys Thr Leu Ile 
        35                  40                  45              


Tyr Arg Ala Asn Arg Leu Val Asp Gly Val Pro Ser Ser Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Gln Asp Tyr Ser Leu Thr Ile Ser Ser Leu Glu 
65                  70                  75                  80  


Tyr Glu Asp Met Gly Ile Tyr Tyr Cys Leu Gln Tyr Asp Glu Phe Pro 
                85                  90                  95      


Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105             


<210>  17
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: heavy chain variable region CDR1 of the anti-ceramide 
       antibody

<400>  17

Gly Tyr Thr Phe Thr Ser Tyr Trp Met His 
1               5                   10  


<210>  18
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: heavy chain variable region CDR2

<400>  18

Tyr Ile Asn Pro Ser Ser Gly Tyr Thr Lys Tyr Asn Gln Phe Lys Asp 
1               5                   10                  15      


<210>  19
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: heavy chain variable region CDR3

<400>  19

Gly Gly Tyr Tyr Gly Phe Ala Tyr 
1               5               


<210>  20
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: light chain variable region CDR1

<400>  20

Ser Ala Ser Ser Ser Val Ser Tyr Met Tyr 
1               5                   10  


<210>  21
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: light chain variable region CDR2

<400>  21

Leu Thr Ser Asn Leu Ala Ser 
1               5           


<210>  22
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: light chain variable region CDR3

<400>  22

Gln Gln Trp Ser Ser Asn Pro Leu Thr 
1               5                   


<210>  23
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: heavy chain variable region sequence

<400>  23

Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Trp Met His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Ser Ser Gly Tyr Thr Lys Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Asp Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Tyr Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly Tyr Tyr Gly Phe Ala Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ala 
        115         


<210>  24
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: light chain variable region sequence

<400>  24

Gln Ile Val Leu Thr Gln Ser Pro Ala Leu Met Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 
            20                  25                  30          


Tyr Trp Tyr Gln Gln Lys Pro Arg Ser Ser Pro Lys Pro Trp Ile Tyr 
        35                  40                  45              


Leu Thr Ser Asn Leu Ala Ser Gly Val Pro Ala Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala Glu 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser Ser Asn Pro Leu Thr 
                85                  90                  95      


Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            100                 105     


<210>  25
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: heavy chain variable region CDR1 of the anti-ceramide 
       antibody

<400>  25

Gly Phe Ser Leu Thr Gly Tyr Gly Val His 
1               5                   10  


<210>  26
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: heavy chain variable region CDR2

<400>  26

Val Ile Trp Ser Gly Gly Ser Thr Asp Tyr Asn Ala Ala Phe Ile Ser 
1               5                   10                  15      


<210>  27
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: heavy chain variable region CDR3

<400>  27

Asn Tyr Gly Tyr Asp Tyr Ala Met Asp Tyr 
1               5                   10  


<210>  28
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: light chain variable region CDR1

<400>  28

Arg Ala Ser Gln Ser Ile Gly Thr Ser Ile His 
1               5                   10      


<210>  29
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: light chain variable region CDR2

<400>  29

Tyr Ala Ser Glu Ser Ile Ser 
1               5           


<210>  30
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: light chain variable region CDR3

<400>  30

Gln Gln Ser Asn Ser Trp Pro Phe Thr 
1               5                   


<210>  31
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: heavy chain variable region sequence

<400>  31

Gln Val Gln Leu Lys Gln Ser Gly Pro Gly Val Gln Pro Ser Ser Leu 
1               5                   10                  15      


Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Thr Ser Tyr Gly Val 
            20                  25                  30          


His Trp Val Arg Gln Ser Pro Gly Lys Gly Leu Glu Trp Leu Gly Val 
        35                  40                  45              


Ile Trp Ser Gly Gly Ser Thr Asp Tyr Asn Ala Ala Phe Ile Ser Arg 
    50                  55                  60                  


Leu Ser Ile Ser Lys Asp Asn Ser Lys Ser Gln Val Phe Phe Lys Met 
65                  70                  75                  80  


Asn Ser Leu Gln Ala Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Arg Asn 
                85                  90                  95      


Tyr Gly Tyr Asp Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser Val 
            100                 105                 110         


Thr Val Ser Ser 
        115     


<210>  32
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: light chain variable region sequence

<400>  32

Asp Ile Leu Leu Thr Gln Ser Pro Ala Ile Leu Ser Val Ser Pro Gly 
1               5                   10                  15      


Glu Arg Val Ser Phe Ser Cys Arg Ala Ser Gln Ser Ile Gly Thr Ser 
            20                  25                  30          


Ile His Trp Tyr Gln Gln Arg Thr Asn Gly Ser Pro Arg Leu Leu Ile 
        35                  40                  45              


Lys Tyr Ala Ser Glu Ser Ile Ser Gly Ile Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Ser Ile Asn Ser Val Glu Ser 
65                  70                  75                  80  


Glu Asp Ile Ala Asp Tyr Tyr Cys Gln Gln Ser Asn Ser Trp Pro Phe 
                85                  90                  95      


Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  33
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: heavy chain variable region CDR1 of the anti-ceramide 
       antibody

<400>  33

Gly Tyr Thr Phe Thr Asn Tyr Trp Met His 
1               5                   10  


<210>  34
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: heavy chain variable region CDR2

<400>  34

Ala Ile Tyr Pro Gly Asp Ser Asp Thr Ser Tyr Asn Gln Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  35
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: heavy chain variable region CDR3

<400>  35

Gly Leu Tyr Tyr Gly Tyr Asp 
1               5           


<210>  36
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: light chain variable region CDR1

<400>  36

Lys Ser Ser Gln Ser Leu Ile Asp Ser Asp Gly Lys Thr Phe Leu Asn 
1               5                   10                  15      


<210>  37
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: light chain variable region CDR2

<400>  37

Leu Val Ser Lys Leu Asp Ser 
1               5           


<210>  38
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: said light chain variable region CDR3

<400>  38

Trp Gln Gly Thr His Phe Pro Tyr Thr 
1               5                   


<210>  39
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: heavy chain variable region sequence comprising  the 
       sequence

<400>  39

Glu Val Gln Leu Gln Gln Ser Gly Thr Val Leu Ala Arg Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Trp Met His Trp Val Lys Gln Arg Pro Val Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Ala Ile Tyr Pro Gly Asp Ser Asp Thr Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Lys Leu Thr Ala Val Thr Ser Thr Ser Thr Ala Phe 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Thr Asn Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Gly Leu Tyr Tyr Gly Tyr Asp Trp Gly Gln Gly Thr Thr Leu Thr 
            100                 105                 110         


Val Ser Ser 
        115 


<210>  40
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: light chain variable region sequence

<400>  40

Asp Val Leu Met Thr Gln Thr Pro Leu Thr Leu Ser Val Thr Ile Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser Leu Ile Asp Ser 
            20                  25                  30          


Asp Gly Lys Thr Phe Leu Asn Trp Leu Leu Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Arg Leu Ile Tyr Leu Val Ser Lys Leu Asp Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Leu Tyr Tyr Cys Trp Gln Gly 
                85                  90                  95      


Thr His Phe Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  41
<211>  109
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  human V gene 1-46

<400>  41

Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Tyr Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Ile Ile Asn Pro Ser Gly Gly Ser Thr Ser Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Gly Arg Val Thr Met Thr Arg Asp Thr Ser Thr Ser Thr Val Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Trp Gly Gln Gly Thr Thr Val Thr Val Ser Ser 
            100                 105                 


<210>  42
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: VL 2A2

<400>  42

Asp Val Leu Met Thr Gln Thr Pro Leu Thr Leu Ser Val Thr Ile Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser Leu Ile Asp Ser 
            20                  25                  30          


Asp Gly Lys Thr Phe Leu Asn Trp Leu Leu Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Arg Leu Ile Tyr Leu Val Ser Lys Leu Asp Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Leu Tyr Tyr Cys Trp Gln Gly 
                85                  90                  95      


Thr His Phe Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


Arg 
    


<210>  43
<211>  112
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  human V gene A1

<400>  43

Asp Val Val Met Thr Gln Ser Pro Leu Ser Leu Pro Val Thr Leu Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Leu Val Tyr Ser 
            20                  25                  30          


Asp Gly Asn Thr Tyr Leu Asn Trp Phe Gln Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Arg Arg Leu Ile Tyr Lys Val Ser Asn Trp Asp Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gln Gly 
                85                  90                  95      


Thr His Trp Pro Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg 
            100                 105                 110         


<210>  44
<211>  1395
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Humanized 2A2 Heavy chain sequence

<400>  44
atggactgga cctggagggt cttctgcttg ctggctgtag ctccaggtgc tcactcccag       60

gtgcagcttg tgcagtctgg ggctgaggtg aaaaagcctg gggcttcagt gaaggtgtcc      120

tgcaaggctt ctggctacac ctttaccaac tactggatgc actgggtaag acaggcgcct      180

ggacagggtc tggaatggat gggcgctatt tatcctggag atagtgatac tagctacaac      240

cagaagttca agggccgggt cacaatgact cgagacacat ccaccagcac tgtctacatg      300

gagctcagca gcctgagaag tgaggacact gcggtctatt actgtgcacg cctttactac      360

ggctacgact ggggccaagg caccactgtc acagtctcct cagccagcac gaagggccca      420

tcggtcttcc ccctggcacc ctcctccaag agcacctctg ggggcacagc ggccctgggc      480

tgcctggtca aggactactt ccccgaaccg gtgacggtgt cgtggaactc aggcgccctg      540

accagcggcg tgcacacctt cccggctgtc ctacagtcct caggactcta ctccctcagc      600

agcgtggtga ccgtgccctc cagcagcttg ggcacccaga cctacatctg caacgtgaat      660

cacaagccca gcaacaccaa ggtggacaag aaagttgagc ccaaatcttg tgacaaaact      720

cacacatgcc caccgtgccc agcacctgaa ctcctggggg gaccgtcagt cttcctcttc      780

cccccaaaac ccaaggacac cctcatgatc tcccggaccc ctgaggtcac atgcgtggtg      840

gtggacgtga gccacgaaga ccctgaggtc aagttcaact ggtacgtgga cggcgtggag      900

gtgcataatg ccaagacaaa gccgcgggag gagcagtaca acagcacgta ccgtgtggtc      960

agcgtcctca ccgtcctgca ccaggactgg ctgaatggca aggagtacaa gtgcaaggtc     1020

tccaacaaag ccctcccagc ccccatcgag aaaaccatct ccaaagccaa agggcagccc     1080

cgagaaccac aggtgtacac cctgccccca tcccgggatg agctgaccaa gaaccaggtc     1140

agcctgacct gcctggtcaa aggcttctat cccagcgaca tcgccgtgga gtgggagagc     1200

aatgggcagc cggagaacaa ctacaagacc acgcctcccg tgctggactc cgacggctcc     1260

ttcttcctct acagcaagct caccgtggac aagagcaggt ggcagcaggg gaacgtcttc     1320

tcatgctccg tgatgcatga ggctctgcac aaccactaca cgcagaagag cctctccctg     1380

tccccgggta aatga                                                      1395


<210>  45
<211>  463
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Humanized 2A2 Heavy chain sequence

<400>  45

Met Asp Trp Thr Trp Arg Val Phe Cys Leu Leu Ala Val Ala Pro Gly 
1               5                   10                  15      


Ala His Ser Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asn Tyr Trp Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu 
    50                  55                  60                  


Glu Trp Met Gly Ala Ile Tyr Pro Gly Asp Ser Asp Thr Ser Tyr Asn 
65                  70                  75                  80  


Gln Lys Phe Lys Gly Arg Val Thr Met Thr Arg Asp Thr Ser Thr Ser 
                85                  90                  95      


Thr Val Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Leu Tyr Tyr Gly Tyr Asp Trp Gly Gln Gly Thr 
        115                 120                 125             


Thr Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
    130                 135                 140                 


Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly 
145                 150                 155                 160 


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
                165                 170                 175     


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
            180                 185                 190         


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
        195                 200                 205             


Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser 
    210                 215                 220                 


Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr 
225                 230                 235                 240 


His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 
                245                 250                 255     


Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 
            260                 265                 270         


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 
        275                 280                 285             


Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
    290                 295                 300                 


Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 
305                 310                 315                 320 


Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
                325                 330                 335     


Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 
            340                 345                 350         


Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
        355                 360                 365             


Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys 
    370                 375                 380                 


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
385                 390                 395                 400 


Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 
                405                 410                 415     


Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 
            420                 425                 430         


Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
        435                 440                 445             


Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
    450                 455                 460             


