                         SEQUENCE LISTING

<110>  KANSAS STATE UNIVERSITY RESEARCH FOUNDATION
 
<120>  NANOSENSORS FOR DETECTING ENZYMATIC ACTIVITY IN DAIRY PRODUCTION

<130>  46528-PCT

<150>  US 62/029,893
<151>  2014-07-28

<160>  85    

<170>  PatentIn version 3.5

<210>  1
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  1

Val Pro Met Ser Met Arg Gly Gly 
1               5               


<210>  2
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  2

Ile Pro Val Ser Leu Arg Ser Gly 
1               5               


<210>  3
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  3

Ser Gly Arg Ser Ala Phe Arg Phe Phe Gly Ala 
1               5                   10      


<210>  4
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  4

Gly Pro Ser Gly Leu Ala Gly Ser Gly Arg Ser Ala 
1               5                   10          


<210>  5
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  5

Ser Gly Pro Gly Arg Ala Gly Gly Ala 
1               5                   


<210>  6
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  6

Arg Pro Phe Ser Met Ile Met Gly 
1               5               


<210>  7
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  7

Val Pro Leu Ser Leu Thr Met Gly 
1               5               


<210>  8
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  8

Gly Pro Ser Gly Leu Arg Gly Ala 
1               5               


<210>  9
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  9

Val Pro Leu Ser Leu Tyr Ser Gly 
1               5               


<210>  10
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  10

His Gly Pro Glu Gly Leu Arg Val Gly Phe Tyr Glu Ser Asp Val Met 
1               5                   10                  15      


Gly Arg Gly His Ala Arg Leu Val His Val Glu Glu Pro His Thr 
            20                  25                  30      


<210>  11
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  11

Gly Ala Ala Asn Leu Val Arg Gly 
1               5               


<210>  12
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  12

Gly Pro Ala Gly Leu Gly Ala Ala 
1               5               


<210>  13
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  13

Gly Pro Gln Gly Leu Ala Gly Gln Arg Gly Ile Val 
1               5                   10          


<210>  14
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  14

Ile Pro Glu Ser Leu Arg Ala Gly 
1               5               


<210>  15
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  15

Ser Gly Arg Ser Ala 
1               5   


<210>  16
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  16

Gly Leu Ala Gly Leu Ala Gly Pro 
1               5               


<210>  17
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  17

Ser Leu Leu Lys Ser Arg Met Val Pro Asn Phe Asn 
1               5                   10          


<210>  18
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  18

Asp Ala Phe Lys 
1               


<210>  19
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  19

Ser Leu Leu Ile Phe Arg Ser Trp Ala Asn Phe Asn 
1               5                   10          


<210>  20
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  20

Ser Gly Lys Pro Ile Leu Phe Phe Arg Leu 
1               5                   10  


<210>  21
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  21

Ser Gly Ser Pro Ala Phe Leu Ala Lys Asn Arg 
1               5                   10      


<210>  22
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  22

Ser Gly Lys Pro Ile Ile Phe Phe Arg Leu 
1               5                   10  


<210>  23
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  23

Pro Arg Ala Gly Ala 
1               5   


<210>  24
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  24

Ser Gly Val Val Ile Ala Thr Val Ile Val Ile Thr 
1               5                   10          


<210>  25
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  25

Gly Pro Leu Gly Met Leu Ser Gln 
1               5               


<210>  26
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  26

Gly Glu Pro Leu Ser Leu Leu Pro 
1               5               


<210>  27
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (7)..(9)
<223>  Each Xaa is any amino acid

<400>  27

Gly Gly Xaa Gly Xaa Asp Xaa Xaa Xaa 
1               5                   


<210>  28
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (6)..(7)
<223>  Each Xaa is any amino acid

<400>  28

Ser Xaa Ala Xaa Ala Xaa Xaa Gln Ala Ser 
1               5                   10  


<210>  29
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  29

Gly Gly Ser Gly Ala Asp Ala Gly Ala 
1               5                   


<210>  30
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  30

Ser Arg Ala Gly Ala Lys Ser Gln Ala Ser 
1               5                   10  


<210>  31
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  31

Ala Ala Ala Ala Ala Ala Ala Ala 
1               5               


<210>  32
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  32

Ser Ser Ser Ser Ser Ser Ser Ser 
1               5               


<210>  33
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  33

Phe Phe Phe Phe Phe Phe Phe Phe 
1               5               


<210>  34
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  34

Gln Gln Gln Gln Gln Gln Gln Gln 
1               5               


<210>  35
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  35

Ala Ser Ala Ser Ala Ser Ala Ser 
1               5               


<210>  36
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  36

Phe Gln Phe Gln Phe Gln Phe Gln 
1               5               


<210>  37
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic protease consensus sequence

<400>  37

Ala Phe Ala Phe Ala Phe Ala Phe 
1               5               


<210>  38
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  38

Gly Gly Gly Cys 
1               


<210>  39
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  39

Ala Ala Ala Cys 
1               


<210>  40
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  40

Ser Ser Ser Cys 
1               


<210>  41
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  41

Thr Thr Thr Cys 
1               


<210>  42
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa is any amino acid other than cysteine or lysine

<400>  42

Gly Xaa Gly Asp 
1               


<210>  43
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa is any amino acid other than cysteine or lysine

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa is any amino acid other than cysteine or lysine

<400>  43

Gly Xaa Gly Xaa Gly Asp 
1               5       


<210>  44
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  44

Ser Arg Ser Arg Ser Arg Ser Arg Ser Arg 
1               5                   10  


<210>  45
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  45

Lys Ser Arg Ser Arg Ser Arg Ser Arg Ser Arg 
1               5                   10      


<210>  46
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  46

Lys Lys Ser Arg Ser Arg Ser Arg Ser Arg Ser Arg 
1               5                   10          


<210>  47
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  47

Cys Gly Gly Gly 
1               


<210>  48
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  48

Lys Gly Gly Gly 
1               


<210>  49
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid other than cysteine or lysine

<400>  49

Lys Gly Xaa Gly 
1               


<210>  50
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid other than cysteine or lysine

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is any amino acid other than cysteine or lysine

<400>  50

Lys Gly Xaa Gly Xaa Gly 
1               5       


<210>  51
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid other than cysteine

<400>  51

Asp Gly Xaa Gly 
1               


<210>  52
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is any amino acid other than cysteine

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is any amino acid other than cysteine

<400>  52

Asp Gly Xaa Gly Xaa Gly 
1               5       


<210>  53
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  53

Lys Gly Gly Val Pro Met Ser Met Arg Gly Gly Gly Cys 
1               5                   10              


<210>  54
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  54

His His His Gly Ala Gly Val Pro Met Ser Met Arg Gly Ala Gly 
1               5                   10                  15  


<210>  55
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  55

Gly Ala Gly Ile Pro Val Ser Leu Arg Ser Gly Ala Gly 
1               5                   10              


<210>  56
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  56

Lys Gly Gly Ile Pro Val Ser Leu Arg Ser Gly Gly Cys 
1               5                   10              


<210>  57
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  57

His His His Gly Ala Gly Ile Pro Val Ser Leu Arg Ser Gly Ala Gly 
1               5                   10                  15      


<210>  58
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  58

His His His Gly Ala Gly Arg Pro Phe Ser Met Ile Met Gly Ala Gly 
1               5                   10                  15      


<210>  59
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  59

Lys Gly Gly Val Pro Leu Ser Leu Thr Met Gly Gly Cys 
1               5                   10              


<210>  60
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  60

His His His Gly Ala Gly Val Pro Leu Ser Leu Thr Met Gly Ala Gly 
1               5                   10                  15      


<210>  61
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  61

Gly Ala Gly Pro Ser Gly Leu Arg Gly Ala Gly 
1               5                   10      


<210>  62
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  62

Gly Ala Gly Val Pro Leu Ser Leu Tyr Ser Gly Ala Gly 
1               5                   10              


<210>  63
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  63

His His His Gly Ala Gly Val Pro Leu Ser Leu Tyr Ser Gly Ala Gly 
1               5                   10                  15      


<210>  64
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  64

His His His Gly Ala Gly Gly Ala Ala Asn Leu Val Arg Gly Gly Ala 
1               5                   10                  15      


Gly 
    


<210>  65
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  65

Gly Ala Gly Pro Ala Gly Leu Gly Ala Ala Gly 
1               5                   10      


<210>  66
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  66

His His His Gly Ala Gly Pro Gln Gly Leu Ala Gly Gln Arg Gly Ile 
1               5                   10                  15      


Val Gly Ala Gly 
            20  


<210>  67
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  67

Gly Ala Gly Ser Arg Gly Ser Ala Gly Ala Gly 
1               5                   10      


<210>  68
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  68

Lys Gly Gly Gly Ser Gly Arg Ser Ala Gly Gly Gly Cys 
1               5                   10              


<210>  69
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  69

Cys Gly Gly Gly Ser Gly Arg Ser Ala Gly Gly Cys 
1               5                   10          


<210>  70
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  70

Cys Gly Gly Gly Ser Gly Arg Ser Ala Gly Gly Gly Cys 
1               5                   10              


<210>  71
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  71

Asp Gly Gly Ser Gly Arg Ser Ala Gly Gly Lys 
1               5                   10      


<210>  72
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  72

Ser Arg Ser Arg Ser Arg Ser Arg Ser Arg Ser Gly Arg Ser Ala Gly 
1               5                   10                  15      


Gly Gly Cys 
            


<210>  73
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  73

Lys Gly Gly Ser Gly Arg Ser Ala Gly Gly Asp 
1               5                   10      


<210>  74
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  74

Cys Gly Gly Gly Ser Gly Arg Ser Ala Gly Gly Gly 
1               5                   10          


<210>  75
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  75

Asp Gly Gly Gly Ser Gly Arg Ser Ala Gly Gly Gly Asp 
1               5                   10              


<210>  76
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  76

Asp Gly Ala Gly Ser Gly Arg Ser Ala Gly Ala Gly Asp 
1               5                   10              


<210>  77
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  77

Lys Gly Gly Ser Gly Arg Ser Ala Gly Gly Gly 
1               5                   10      


<210>  78
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  78

Asp Gly Gly Ser Gly Arg Ser Ala Gly Gly Gly Cys 
1               5                   10          


<210>  79
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  79

His His His Gly Ala Gly Ser Gly Arg Ser Ala Gly Ala Gly 
1               5                   10                  


<210>  80
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  80

Gly Ala Gly Ser Leu Leu Lys Ser Arg Met Val Pro Asn Phe Asn Ala 
1               5                   10                  15      


Gly 
    


<210>  81
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  81

His His His Gly Ala Gly Ser Leu Leu Lys Ser Arg Met Val Pro Asn 
1               5                   10                  15      


Phe Asn Gly Ala Gly 
            20      


<210>  82
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  82

His His His Gly Ala Gly Ser Leu Leu Ile Phe Arg Ser Trp Ala Asn 
1               5                   10                  15      


Phe Asn Gly Ala Gly 
            20      


<210>  83
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  83

His His His Gly Ala Gly Ser Gly Val Val Ile Ala Thr Val Ile Val 
1               5                   10                  15      


Ile Thr Gly Ala Gly 
            20      


<210>  84
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  84

His His His Gly Ala Gly Pro Arg Ala Gly Ala Gly 
1               5                   10          


<210>  85
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic oligopeptide construct with consensus sequence

<400>  85

Gly Ala Gly Glu Pro Leu Ser Leu Leu Pro Ala Gly 
1               5                   10          


