               SEQUENCE LISTING

<110> Symrise AG

<120> Verfahren zur biotechnologischen Herstellung von Flavonglykosid-Dihydrochalconen

<130> C 3275 EP

<140> EP14175312.9
<141> 2014-07-01

<150> EP14175312.9
<151> 2014-07-01

<160> 9

<170> BiSSAP 1.3

<210> 1
<211> 852
<212> DNA
<213> Eubacterium ramulus

<400> 1
atggcagatt tcaaattcga accaatgaga agtcttatct acgttgactg cgtaagcgaa      60

gactacagac caaaacttca gagatggatt tataaagtac atattccgga cagcatctct     120

cagtttgagc cgtatgttac caaatatgca ttttatccgt ccttcccgat tccaccacag     180

ggtgatcgtt tcggatacgc aagaatgcag ctgacagagc atcactggtt agtaagcgac     240

cttgatcctc gtcttgagat caaagcaatc gctgagacat tcccgatgga cgtacttgta     300

tggcagggac agatcccggc agcagctcat acagacgctc agatcgattc tgacggagat     360

gcaggaaatg cagcccgtaa atccaacaat gcagaaggaa atccatttat ctttgcattc     420

cttccgatgt ggtgggagaa agacctgaaa ggaaaaggac gtacgatcga ggacggcgca     480

aactatcgtt tcaatatgac tatcggtttc ccagaaggcg tagacaaagc agagggagag     540

aaatggttat ttgagaaagt agttccgatt cttcaggcag ctccggagtg tacacgtgta     600

cttgcaagtg ccgtaaagaa agacatcaac ggatgcgtaa tggattgggt acttgaaatc     660

tggtttgaga atcagtccgg atggtacaaa gtaatggtag atgacatgaa agcacttgaa     720

aaaccgtcat gggctcagca ggatgctttc ccgttcctga aaccatacca caatgtttgc     780

agtgcagcag ttgctgatta tacaccaagc aacaaccttg caaattatcg tggatacatc     840

accatgagat aa                                                         852


<210> 2
<211> 283
<212> PRT
<213> Eubacterium ramulus

<400> 2
Met Ala Asp Phe Lys Phe Glu Pro Met Arg Ser Leu Ile Tyr Val Asp 
1               5                   10                  15      
Cys Val Ser Glu Asp Tyr Arg Pro Lys Leu Gln Arg Trp Ile Tyr Lys 
            20                  25                  30          
Val His Ile Pro Asp Ser Ile Ser Gln Phe Glu Pro Tyr Val Thr Lys 
        35                  40                  45              
Tyr Ala Phe Tyr Pro Ser Phe Pro Ile Pro Pro Gln Gly Asp Arg Phe 
    50                  55                  60                  
Gly Tyr Ala Arg Met Gln Leu Thr Glu His His Trp Leu Val Ser Asp 
65                  70                  75                  80  
Leu Asp Pro Arg Leu Glu Ile Lys Ala Ile Ala Glu Thr Phe Pro Met 
                85                  90                  95      
Asp Val Leu Val Trp Gln Gly Gln Ile Pro Ala Ala Ala His Thr Asp 
            100                 105                 110         
Ala Gln Ile Asp Ser Asp Gly Asp Ala Gly Asn Ala Ala Arg Lys Ser 
        115                 120                 125             
Asn Asn Ala Glu Gly Asn Pro Phe Ile Phe Ala Phe Leu Pro Met Trp 
    130                 135                 140                 
Trp Glu Lys Asp Leu Lys Gly Lys Gly Arg Thr Ile Glu Asp Gly Ala 
145                 150                 155                 160 
Asn Tyr Arg Phe Asn Met Thr Ile Gly Phe Pro Glu Gly Val Asp Lys 
                165                 170                 175     
Ala Glu Gly Glu Lys Trp Leu Phe Glu Lys Val Val Pro Ile Leu Gln 
            180                 185                 190         
Ala Ala Pro Glu Cys Thr Arg Val Leu Ala Ser Ala Val Lys Lys Asp 
        195                 200                 205             
Ile Asn Gly Cys Val Met Asp Trp Val Leu Glu Ile Trp Phe Glu Asn 
    210                 215                 220                 
Gln Ser Gly Trp Tyr Lys Val Met Val Asp Asp Met Lys Ala Leu Glu 
225                 230                 235                 240 
Lys Pro Ser Trp Ala Gln Gln Asp Ala Phe Pro Phe Leu Lys Pro Tyr 
                245                 250                 255     
His Asn Val Cys Ser Ala Ala Val Ala Asp Tyr Thr Pro Ser Asn Asn 
            260                 265                 270         
Leu Ala Asn Tyr Arg Gly Tyr Ile Thr Met Arg 
        275                 280             

<210> 3
<211> 66
<212> DNA
<213> Eubacterium ramulus

<400> 3
catacagacg ctcagatcga ttctgacgga gatgcaggaa atgcagcccg taaatccaac     60

aatgca                                                                66


<210> 4
<211> 22
<212> PRT
<213> Eubacterium ramulus

<400> 4
His Thr Asp Ala Gln Ile Asp Ser Asp Gly Asp Ala Gly Asn Ala Ala 
1               5                   10                  15      
Arg Lys Ser Asn Asn Ala 
            20          

<210> 5
<211> 2037
<212> DNA
<213> Eubacterium ramulus

<400> 5
atggcagaaa aaaatcagta ttttccacat ttgtttgagc cgttaaaagt tggttcaaag      60

acaattaaga accgtattga ggcagcaccg gctttatttg cattcgagca ttatatcgaa     120

ctgaatccgg atccgtttgg ctataccaca ccggttccgg agcgtgcgtt ccgtatgctg     180

gaggcaaagg caaaaggagg ggcaggaatt gtatgtctgg gtgagttaag cccgaatcat     240

gagtatgaca aacggtttcc gtttgaaccg tatcttgatt ttacatccag atcagataag     300

cagtttgaaa ttatgaagga aactgcggag atgatcaaaa gctatggggc atttccgatg     360

ggcgagctgc tttcctgcgg tgaaatcaag acaaatatcg gagatggtat caatccgaag     420

ggaccatctg agaaagatct tccggatggc tctcatgtgg aggcgtttac aaaagaagag     480

attttaagct gctatcagga ttatgtaact gcatgtaaat ggtttcaggc agcaggctgg     540

gaaggcatta tgatccactg cggacatggc tggcttccgg cacagttcct gtctccgcaa     600

tacaataaac gtaccgatga gtatggtgga tcttttgaaa acagagcaag atttactgtt     660

gatctgttaa aaactgttcg tgaagctatg ggaccggact tcgtgatcga gatccgtgtc     720

agcagctctg agcatttacc gggcggatta gagctggaag atgctgtaaa ttattgtaaa     780

ctgtgtgagc catacattga tatgatccat gtctcctgtg gtcattacct gagttcttcc     840

agaagttggg agttcacaac tgcttatgca ccgcatggtc cgaatattga accggcagct     900

gttatcaaac agaacgtatc cattccggtt gcggcagtcg gcggcatcaa ttctccggaa     960

caggcggaag aggcaatcgc gtcaggaaaa atcgatatgg tatccatggg acgtcagttc    1020

tttgcagatc cggcatttcc aaacaaggca aaagaagggc atgcagatga gatccgtcgc    1080

tgtctgcgct gcggaagatg ctatccgggt ccgtccggcg agcatgaaac agagatctgg    1140

acggtgaaat tcccaccact ggattcctgt accatcaatc catatgatgt atggccggca    1200

tctcatcata aagtccttcc ggaccgcatg ccgaaaccgg aagcaagccg taaggtattg    1260

gtagtaggcg gcggctgtgg cggtctgcag acagcgatca cagcatcaga cagaggtcat    1320

caggtaatcc tgtgcgaaaa atccggagta ttaggcggtc tgatcaattt tacggatcat    1380

acggatcata aagtagatat cagaaacttc aaagatctgc tgatccgcga tgtggagaaa    1440

cgtccgatcg aagtaagatt aaactgtgaa gtaacaccgg aactcatcag agaaattgct    1500

ccggaagcag ttgtactggc cgtcggatcc gatgatctga tccttccaat cgagggaatt    1560

gaaaatgcgg taacagcaat ggacgtatac agcaatgact ttgcaggtct tggaaagagc    1620

accatcgtac tcggtggcgg tctggttggc tgtgaggcag ccgcagatta tattgatcac    1680

ggtgtagaga caacgattgt tgaaatgaaa ggtgcgctga tgccggagac aaccggtctg    1740

taccgtacag ctgtacatga tttcatcgac aaaaacggcg gcaaatacga agtaaatgca    1800

aaagttgtca aagttggcaa agattttgtg gtagcggaac aagatgggaa agagattacc    1860

atcaaagcag attctgttgt caatgcaatg ggacgccgtg cgcatgcgac agaagcactt    1920

gagacagcta tcaaagaagc tggtattccg gtatggaaga tcggtgactg tgtccgtgcg    1980

cgtcagatcg gtgatgcggt aagagaaggc tggaccgcag caatggaaat tatctaa       2037


<210> 6
<211> 678
<212> PRT
<213> Eubacterium ramulus

<400> 6
Met Ala Glu Lys Asn Gln Tyr Phe Pro His Leu Phe Glu Pro Leu Lys 
1               5                   10                  15      
Val Gly Ser Lys Thr Ile Lys Asn Arg Ile Glu Ala Ala Pro Ala Leu 
            20                  25                  30          
Phe Ala Phe Glu His Tyr Ile Glu Leu Asn Pro Asp Pro Phe Gly Tyr 
        35                  40                  45              
Thr Thr Pro Val Pro Glu Arg Ala Phe Arg Met Leu Glu Ala Lys Ala 
    50                  55                  60                  
Lys Gly Gly Ala Gly Ile Val Cys Leu Gly Glu Leu Ser Pro Asn His 
65                  70                  75                  80  
Glu Tyr Asp Lys Arg Phe Pro Phe Glu Pro Tyr Leu Asp Phe Thr Ser 
                85                  90                  95      
Arg Ser Asp Lys Gln Phe Glu Ile Met Lys Glu Thr Ala Glu Met Ile 
            100                 105                 110         
Lys Ser Tyr Gly Ala Phe Pro Met Gly Glu Leu Leu Ser Cys Gly Glu 
        115                 120                 125             
Ile Lys Thr Asn Ile Gly Asp Gly Ile Asn Pro Lys Gly Pro Ser Glu 
    130                 135                 140                 
Lys Asp Leu Pro Asp Gly Ser His Val Glu Ala Phe Thr Lys Glu Glu 
145                 150                 155                 160 
Ile Leu Ser Cys Tyr Gln Asp Tyr Val Thr Ala Cys Lys Trp Phe Gln 
                165                 170                 175     
Ala Ala Gly Trp Glu Gly Ile Met Ile His Cys Gly His Gly Trp Leu 
            180                 185                 190         
Pro Ala Gln Phe Leu Ser Pro Gln Tyr Asn Lys Arg Thr Asp Glu Tyr 
        195                 200                 205             
Gly Gly Ser Phe Glu Asn Arg Ala Arg Phe Thr Val Asp Leu Leu Lys 
    210                 215                 220                 
Thr Val Arg Glu Ala Met Gly Pro Asp Phe Val Ile Glu Ile Arg Val 
225                 230                 235                 240 
Ser Ser Ser Glu His Leu Pro Gly Gly Leu Glu Leu Glu Asp Ala Val 
                245                 250                 255     
Asn Tyr Cys Lys Leu Cys Glu Pro Tyr Ile Asp Met Ile His Val Ser 
            260                 265                 270         
Cys Gly His Tyr Leu Ser Ser Ser Arg Ser Trp Glu Phe Thr Thr Ala 
        275                 280                 285             
Tyr Ala Pro His Gly Pro Asn Ile Glu Pro Ala Ala Val Ile Lys Gln 
    290                 295                 300                 
Asn Val Ser Ile Pro Val Ala Ala Val Gly Gly Ile Asn Ser Pro Glu 
305                 310                 315                 320 
Gln Ala Glu Glu Ala Ile Ala Ser Gly Lys Ile Asp Met Val Ser Met 
                325                 330                 335     
Gly Arg Gln Phe Phe Ala Asp Pro Ala Phe Pro Asn Lys Ala Lys Glu 
            340                 345                 350         
Gly His Ala Asp Glu Ile Arg Arg Cys Leu Arg Cys Gly Arg Cys Tyr 
        355                 360                 365             
Pro Gly Pro Ser Gly Glu His Glu Thr Glu Ile Trp Thr Val Lys Phe 
    370                 375                 380                 
Pro Pro Leu Asp Ser Cys Thr Ile Asn Pro Tyr Asp Val Trp Pro Ala 
385                 390                 395                 400 
Ser His His Lys Val Leu Pro Asp Arg Met Pro Lys Pro Glu Ala Ser 
                405                 410                 415     
Arg Lys Val Leu Val Val Gly Gly Gly Cys Gly Gly Leu Gln Thr Ala 
            420                 425                 430         
Ile Thr Ala Ser Asp Arg Gly His Gln Val Ile Leu Cys Glu Lys Ser 
        435                 440                 445             
Gly Val Leu Gly Gly Leu Ile Asn Phe Thr Asp His Thr Asp His Lys 
    450                 455                 460                 
Val Asp Ile Arg Asn Phe Lys Asp Leu Leu Ile Arg Asp Val Glu Lys 
465                 470                 475                 480 
Arg Pro Ile Glu Val Arg Leu Asn Cys Glu Val Thr Pro Glu Leu Ile 
                485                 490                 495     
Arg Glu Ile Ala Pro Glu Ala Val Val Leu Ala Val Gly Ser Asp Asp 
            500                 505                 510         
Leu Ile Leu Pro Ile Glu Gly Ile Glu Asn Ala Val Thr Ala Met Asp 
        515                 520                 525             
Val Tyr Ser Asn Asp Phe Ala Gly Leu Gly Lys Ser Thr Ile Val Leu 
    530                 535                 540                 
Gly Gly Gly Leu Val Gly Cys Glu Ala Ala Ala Asp Tyr Ile Asp His 
545                 550                 555                 560 
Gly Val Glu Thr Thr Ile Val Glu Met Lys Gly Ala Leu Met Pro Glu 
                565                 570                 575     
Thr Thr Gly Leu Tyr Arg Thr Ala Val His Asp Phe Ile Asp Lys Asn 
            580                 585                 590         
Gly Gly Lys Tyr Glu Val Asn Ala Lys Val Val Lys Val Gly Lys Asp 
        595                 600                 605             
Phe Val Val Ala Glu Gln Asp Gly Lys Glu Ile Thr Ile Lys Ala Asp 
    610                 615                 620                 
Ser Val Val Asn Ala Met Gly Arg Arg Ala His Ala Thr Glu Ala Leu 
625                 630                 635                 640 
Glu Thr Ala Ile Lys Glu Ala Gly Ile Pro Val Trp Lys Ile Gly Asp 
                645                 650                 655     
Cys Val Arg Ala Arg Gln Ile Gly Asp Ala Val Arg Glu Gly Trp Thr 
            660                 665                 670         
Ala Ala Met Glu Ile Ile 
        675             

<210> 7
<211> 2037
<212> DNA
<213> Eubacterium ramulus

<400> 7
atggcagaaa agaaccaata cttcccgcac ctgtttgaac cgctgaaagt cggctctaaa      60

accattaaaa atcgcatcga agcagcaccg gccctgtttg cattcgaaca ttatatcgaa     120

ctgaacccgg acccgtttgg ttacaccacg ccggtgccgg aacgtgcatt ccgtatgctg     180

gaagccaaag caaaaggcgg tgccggcatt gtttgtctgg gtgaactgag cccgaatcac     240

gaatatgata aacgctttcc gttcgaaccg tacctggatt ttaccagccg ttctgacaaa     300

cagttcgaaa ttatgaaaga aacggcagaa atgatcaaaa gctatggcgc ttttccgatg     360

ggtgaactgc tgtcgtgcgg tgaaatcaaa accaacattg gcgatggtat caatccgaaa     420

ggcccgtcag aaaaagatct gccggacggt tcgcatgtgg aagccttcac caaagaagaa     480

atcctgtcat gttaccagga ttacgttacg gcatgcaaat ggttccaagc ggccggctgg     540

gaaggtatta tgatccattg tggccacggt tggctgccgg cgcagtttct gagcccgcaa     600

tataacaaac gcaccgatga atacggcggt tcttttgaaa atcgtgcgcg cttcaccgtc     660

gatctgctga aaacggtgcg tgaagcgatg ggcccggact tcgtgattga aatccgtgtt     720

agctctagtg aacatctgcc gggcggtctg gaactggaag atgcggtgaa ctattgcaaa     780

ctgtgtgaac cgtacattga catgatccat gttagttgcg gccactatct gtcctcatcg     840

cgctcctggg aatttaccac ggcttacgcg ccgcacggtc cgaacatcga accggcagct     900

gtcattaaac agaatgtgag catcccggtt gcagcagtcg gcggtatcaa ctctccggaa     960

caagcggaag aagccattgc aagtggcaaa atcgatatgg ttagcatggg ccgtcagttt    1020

ttcgctgacc cggcgtttcc gaataaagca aaagaaggcc atgctgatga aattcgtcgc    1080

tgcctgcgtt gtggtcgctg ctatccgggc ccgagtggtg aacacgaaac cgaaatctgg    1140

acggtgaaat tcccgccgct ggatagttgt accattaacc cgtacgacgt gtggccggca    1200

tcccatcaca aagttctgcc ggatcgcatg ccgaaaccgg aagcgtcccg taaagttctg    1260

gtggttggcg gtggctgtgg tggtctgcag accgcaatca cggcatcaga ccgcggccat    1320

caagtcattc tgtgcgaaaa atcgggtgtg ctgggtggcc tgattaactt taccgatcat    1380

acggaccaca aagttgatat tcgcaatttc aaagatctgc tgatccgtga cgtcgaaaaa    1440

cgcccgattg aagttcgtct gaattgtgaa gtcaccccgg aactgattcg tgaaatcgct    1500

ccggaagcag tcgtgctggc agtgggcagt gatgacctga ttctgccgat cgaaggtatt    1560

gaaaacgccg ttaccgcaat ggatgtctat agcaatgact ttgccggcct gggtaaatct    1620

acgatcgtgc tgggtggcgg tctggttggc tgcgaagcag ctgcggatta tatcgatcat    1680

ggcgtggaaa ccacgattgt tgaaatgaaa ggcgcactga tgccggaaac cacgggtctg    1740

tatcgtaccg ctgtgcacga ttttattgac aaaaacggcg gtaaatacga agtcaatgcc    1800

aaagttgtca aagtgggcaa agatttcgtg gttgcagaac aggacggtaa agaaattacc    1860

atcaaagcgg attctgtcgt gaatgcgatg ggccgtcgcg ctcacgcaac cgaagctctg    1920

gaaacggcga ttaaagaagc cggcatcccg gtttggaaaa ttggtgattg cgtccgtgcc    1980

cgccaaatcg gtgacgcagt tcgtgaaggc tggacggctg caatggaaat catctaa       2037


<210> 8
<211> 27
<212> DNA
<213> Eubacterium ramulus

<220> 
<223> Forward primer

<400> 8
gatcccggca gcagcagaag gaaatcc                                        27


<210> 9
<211> 27
<212> DNA
<213> Eubacterium ramulus

<220> 
<223> Reverse Primer

<400> 9
ggatttcctt ctgctgctgc cgggatc                                        27


