                               SEQUENCE LISTING

<110> LS9, INC.
 
<120> MICROBIAL PRODUCTION OF FATTY DIOLS

<130> LS00052PCT

<140>
<141>

<150> 62/026,573
<151> 2014-07-18

<160> 8     

<170> PatentIn version 3.5

<210> 1
<211> 342
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      AAR Variant polypeptide"

<400> 1
Met Ala Phe Gly Leu Ile Gly His Ala Thr Ser Leu Glu Gln Ala Arg 
1               5                   10                  15      


Asp Val Trp Arg Arg Leu Gly Tyr Asp Glu Tyr Ala Asp Gln Gly Leu 
            20                  25                  30          


Glu Phe Trp Ser Ser Ala Pro Pro Gln Ile Val Asp Glu Ile Thr Val 
        35                  40                  45              


Thr Ser Ala Thr Gly Lys Val Ile His Gly Arg Tyr Ile Glu Ser Gly 
    50                  55                  60                  


Phe Leu Pro Glu Met Leu Ala Ala Arg Arg Phe Lys Thr Ala Thr Arg 
65                  70                  75                  80  


Lys Val Leu Asn Ala Met Ser His Ala Gln Lys His Gly Ile Asp Ile 
                85                  90                  95      


Ser Ala Leu Gly Gly Phe Thr Ser Ile Ile Phe Glu Asn Phe Asp Leu 
            100                 105                 110         


Ala Lys Leu Arg Gln Val Arg Asp Thr Thr Leu Glu Phe Glu Arg Phe 
        115                 120                 125             


Thr Thr Gly Asn Thr His Thr Ala Tyr Val Ile Cys Arg Gln Val Glu 
    130                 135                 140                 


Ala Ala Ala Lys Thr Leu Gly Ile Asp Ile Ala Gln Ala Thr Val Ala 
145                 150                 155                 160 


Val Val Gly Ala Thr Gly Asp Ile Gly Ser Ala Val Cys Arg Trp Leu 
                165                 170                 175     


Asp Leu Lys Leu Gly Val Gly Asp Leu Ile Leu Thr Ala Arg Asn Gln 
            180                 185                 190         


Glu Arg Leu Asp Asn Leu Gln Ala Glu Leu Gly Arg Gly Lys Ile Leu 
        195                 200                 205             


Pro Leu Glu Ala Ala Leu Pro Glu Ala Asp Phe Ile Val Trp Val Ala 
    210                 215                 220                 


Ser Met Pro Gln Gly Val Val Ile Asp Pro Ala Thr Leu Lys Gln Pro 
225                 230                 235                 240 


Cys Val Leu Ile Asp Gly Gly Tyr Pro Lys Asn Leu Gly Ser Lys Val 
                245                 250                 255     


Gln Gly Glu Gly Ile Tyr Val Leu Asn Gly Gly Val Val Glu His Cys 
            260                 265                 270         


Phe Asp Ile Asp Trp Gln Ile Met Ser Leu Ala Glu Met Ala Arg Pro 
        275                 280                 285             


Glu Arg Gln Met Phe Ala Cys Phe Ala Glu Ala Met Leu Leu Glu Phe 
    290                 295                 300                 


Glu Gly Trp His Thr Asn Phe Ser Trp Gly Arg Asn Gln Ile Thr Ile 
305                 310                 315                 320 


Glu Lys Met Glu Ala Ile Gly Glu Ala Ser Val Arg His Gly Phe Gln 
                325                 330                 335     


Pro Leu Ala Leu Ala Ile 
            340         


<210> 2
<211> 1029
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      AAR Variant polynucleotide"

<400> 2
atggcattcg gtcttatcgg tcatgcaacc agtttggagc aggcccgcga cgtttggcgc       60

aggctgggct acgacgaata cgccgatcaa ggattggagt tttggagtag cgctcctcct      120

caaatcgttg atgaaatcac agtcaccagt gccacaggca aggtgattca cggtcgctac      180

atcgaatcgg ggttcttgcc ggaaatgctg gcggcgcgcc gcttcaaaac agcaacgcgc      240

aaagttctca atgccatgtc ccatgcccaa aaacacggca tcgacatctc ggccttgggg      300

ggctttacct cgattatttt cgagaatttc gatttggcca agttgcggca agtgcgcgac      360

actaccttgg agtttgaacg gttcaccacc ggcaatactc acacggccta cgtaatctgt      420

agacaggtgg aagccgctgc taaaacgctg ggcatcgaca ttgcgcaagc gacagtagcg      480

gttgtcggcg cgactggcga tatcggtagc gctgtctgcc gctggctcga cctcaaactg      540

ggtgtcggtg atttgatcct gacggcgcgc aatcaggagc gtttggataa cctgcaggct      600

gaactcggcc ggggcaagat tctgcccttg gaagccgctc tgccggaagc tgactttatc      660

gtgtgggtcg ccagtatgcc tcagggcgta gtgatcgacc cagcaaccct gaagcaaccc      720

tgcgtcctaa tcgacggggg ctaccccaaa aacttgggca gcaaagtcca aggtgagggc      780

atctatgtcc tcaatggcgg ggtagttgaa cattgcttcg acatcgactg gcagatcatg      840

tccttggcag agatggcgcg gcccgagcgc cagatgtttg cctgctttgc cgaggcgatg      900

ctcttggaat ttgaaggctg gcatactaac ttctcctggg gccgcaacca aatcacgatc      960

gagaagatgg aagcgatcgg tgaggcatcg gtgcgccacg gcttccaacc cttggcattg     1020

gcaatttga                                                             1029


<210> 3
<211> 1174
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      CAR Variant polypeptide"

<400> 3
Met Gly Thr Ser Asp Val His Asp Ala Thr Asp Gly Val Thr Glu Thr 
1               5                   10                  15      


Ala Leu Arg Asp Arg Gln Arg Thr Arg Arg Ile Ala Glu Leu Tyr Ala 
            20                  25                  30          


Thr Asp Pro Glu Phe Ala Ala Ala Ala Pro Leu Pro Ala Val Val Asp 
        35                  40                  45              


Ala Ala His Lys Pro Gly Leu Arg Leu Ala Glu Ile Leu Gln Thr Leu 
    50                  55                  60                  


Phe Thr Gly Tyr Gly Asp Arg Pro Ala Leu Gly Tyr Arg Ala Arg Glu 
65                  70                  75                  80  


Leu Ala Thr Asp Glu Gly Gly Arg Thr Val Thr Arg Leu Leu Pro Arg 
                85                  90                  95      


Phe Asp Thr Leu Thr Tyr Ala Gln Val Trp Ser Arg Val Gln Ala Val 
            100                 105                 110         


Ala Ala Ala Leu Arg His Asn Phe Ala Gln Pro Ile Tyr Pro Gly Asp 
        115                 120                 125             


Ala Val Ala Thr Ile Gly Phe Ala Ser Pro Asp Tyr Leu Thr Leu Asp 
    130                 135                 140                 


Leu Val Cys Ala Tyr Leu Gly Leu Val Ser Val Pro Leu Gln His Asn 
145                 150                 155                 160 


Ala Pro Val Ser Arg Leu Ala Pro Ile Leu Ala Glu Val Glu Pro Arg 
                165                 170                 175     


Ile Leu Thr Val Ser Ala Glu Tyr Leu Asp Leu Ala Val Glu Ser Val 
            180                 185                 190         


Arg Asp Val Asn Ser Val Ser Gln Leu Val Val Phe Asp His His Pro 
        195                 200                 205             


Glu Val Asp Asp His Arg Asp Ala Leu Ala Arg Ala Arg Glu Gln Leu 
    210                 215                 220                 


Ala Gly Lys Gly Ile Ala Val Thr Thr Leu Asp Ala Ile Ala Asp Glu 
225                 230                 235                 240 


Gly Ala Gly Leu Pro Ala Glu Pro Ile Tyr Thr Ala Asp His Asp Gln 
                245                 250                 255     


Arg Leu Ala Met Ile Leu Tyr Thr Ser Gly Ser Thr Gly Ala Pro Lys 
            260                 265                 270         


Gly Ala Met Tyr Thr Glu Ala Met Val Ala Arg Leu Trp Thr Met Ser 
        275                 280                 285             


Gly Ile Thr Gly Asp Pro Thr Pro Val Ile Asn Val Asn Phe Met Pro 
    290                 295                 300                 


Leu Asn His Leu Gly Gly Arg Ile Pro Ile Ser Thr Ala Val Gln Asn 
305                 310                 315                 320 


Gly Gly Thr Ser Tyr Phe Val Pro Glu Ser Asp Met Ser Thr Leu Phe 
                325                 330                 335     


Glu Asp Leu Ala Leu Val Arg Pro Thr Glu Leu Gly Leu Val Pro Arg 
            340                 345                 350         


Val Ala Asp Met Leu Tyr Gln His His Leu Ala Thr Val Asp Arg Leu 
        355                 360                 365             


Val Thr Gln Gly Ala Asp Glu Leu Thr Ala Glu Lys Gln Ala Gly Ala 
    370                 375                 380                 


Glu Leu Arg Glu Gln Val Leu Gly Gly Arg Val Ile Thr Gly Phe Val 
385                 390                 395                 400 


Ser Thr Ala Pro Leu Ala Ala Glu Met Arg Ala Phe Leu Asp Ile Thr 
                405                 410                 415     


Leu Gly Ala His Ile Val Asp Gly Tyr Gly Leu Thr Glu Thr Gly Ala 
            420                 425                 430         


Val Thr Arg Asp Gly Val Ile Val Arg Pro Pro Val Ile Asp Tyr Lys 
        435                 440                 445             


Leu Ile Asp Val Pro Glu Leu Gly Tyr Phe Ser Thr Asp Lys Pro Tyr 
    450                 455                 460                 


Pro Arg Gly Glu Leu Leu Val Arg Ser Ile Thr Leu Thr Pro Gly Tyr 
465                 470                 475                 480 


Tyr Lys Arg Pro Glu Val Thr Ala Ser Val Phe Asp Arg Asp Gly Tyr 
                485                 490                 495     


Tyr His Thr Gly Asp Val Met Ala Glu Thr Ala Pro Asp His Leu Val 
            500                 505                 510         


Tyr Val Asp Arg Arg Asn Asn Val Leu Lys Leu Ala Gln Gly Glu Phe 
        515                 520                 525             


Val Ala Val Ala Asn Leu Glu Ser Val Phe Ser Gly Ala Ala Leu Val 
    530                 535                 540                 


Arg Gln Ile Phe Val Tyr Gly Asn Ser Glu Arg Ser Phe Leu Leu Ala 
545                 550                 555                 560 


Val Val Val Pro Thr Pro Glu Ala Leu Glu Gln Tyr Asp Pro Ala Ala 
                565                 570                 575     


Leu Lys Ala Ala Leu Ala Asp Ser Leu Gln Arg Thr Ala Arg Asp Ala 
            580                 585                 590         


Glu Leu Gln Ser Tyr Glu Val Pro Ala Asp Phe Ile Val Glu Thr Glu 
        595                 600                 605             


Pro Phe Ser Ala Ala Asn Gly Leu Leu Ser Gly Val Gly Lys Leu Leu 
    610                 615                 620                 


Arg Pro Asn Leu Lys Asp Arg Tyr Gly Gln Arg Leu Glu Gln Met Tyr 
625                 630                 635                 640 


Ala Asp Ile Ala Ala Thr Gln Ala Asn Gln Leu Arg Glu Leu Arg Arg 
                645                 650                 655     


Ala Ala Ala Thr Gln Pro Val Ile Asp Thr Leu Thr Gln Ala Ala Ala 
            660                 665                 670         


Thr Ile Leu Gly Thr Gly Ser Glu Val Ala Ser Asp Ala His Phe Thr 
        675                 680                 685             


Asp Leu Gly Gly Asp Ser Leu Ser Ala Leu Thr Leu Ser Asn Leu Leu 
    690                 695                 700                 


Ser Asp Phe Phe Gly Phe Glu Val Pro Val Gly Thr Ile Val Asn Pro 
705                 710                 715                 720 


Ala Thr Asn Leu Ala Gln Leu Ala Gln His Ile Glu Ala Gln Arg Thr 
                725                 730                 735     


Ala Gly Asp Arg Arg Pro Ser Phe Thr Thr Val His Gly Ala Asp Ala 
            740                 745                 750         


Thr Glu Ile Arg Ala Ser Glu Leu Thr Leu Asp Lys Phe Ile Asp Ala 
        755                 760                 765             


Glu Thr Leu Arg Ala Ala Pro Gly Leu Pro Lys Val Thr Thr Glu Pro 
    770                 775                 780                 


Arg Thr Val Leu Leu Ser Gly Ala Asn Gly Trp Leu Gly Arg Phe Leu 
785                 790                 795                 800 


Thr Leu Gln Trp Leu Glu Arg Leu Ala Pro Val Gly Gly Thr Leu Ile 
                805                 810                 815     


Thr Ile Val Arg Gly Arg Asp Asp Ala Ala Ala Cys Ala Arg Leu Thr 
            820                 825                 830         


Gln Ala Tyr Asp Thr Asp Pro Glu Leu Ser Arg Arg Phe Ala Glu Leu 
        835                 840                 845             


Ala Asp Arg His Leu Arg Val Val Ala Gly Asp Ile Gly Asp Gln Asn 
    850                 855                 860                 


Leu Gly Leu Thr Pro Glu Leu Trp His Arg Leu Ala Ala Glu Val Asp 
865                 870                 875                 880 


Leu Val Val His Pro Ala Ala Leu Val Asn His Val Leu Pro Tyr Arg 
                885                 890                 895     


Gln Leu Phe Gly Pro Asn Val Val Gly Thr Ala Glu Val Ile Lys Leu 
            900                 905                 910         


Ala Leu Thr Glu Arg Ile Lys Pro Val Thr Tyr Leu Ser Thr Ala Lys 
        915                 920                 925             


Val Ala Met Gly Ile Pro Asp Phe Glu Glu Asp Gly Asp Ile Arg Thr 
    930                 935                 940                 


Val Ser Pro Val Arg Pro Leu Asp Gly Gly Tyr Ala Asn Gly Tyr Gly 
945                 950                 955                 960 


Asn Ser Lys Trp Ala Gly Glu Val Leu Leu Arg Glu Ala His Asp Leu 
                965                 970                 975     


Cys Gly Leu Pro Val Ala Thr Phe Arg Ser Asp Met Ile Leu Ala His 
            980                 985                 990         


Pro Arg Tyr Arg Gly Gln Val Asn  Val Pro Asp Met Phe  Thr Arg Leu 
        995                 1000                 1005             


Leu Leu  Ser Leu Leu Ile Thr  Gly Val Ala Pro Arg  Ser Phe Tyr 
    1010                 1015                 1020             


Ile Gly  Asp Gly Glu Arg Pro  Arg Ala His Tyr Pro  Gly Leu Thr 
    1025                 1030                 1035             


Val Asp  Phe Val Ala Glu Ala  Val Thr Thr Leu Gly  Ala Gln Gln 
    1040                 1045                 1050             


Arg Glu  Gly Tyr Val Ser Tyr  Asp Val Met Asn Pro  His Asp Asp 
    1055                 1060                 1065             


Gly Ile  Ser Leu Asp Val Phe  Val Asp Trp Leu Ile  Arg Ala Gly 
    1070                 1075                 1080             


His Pro  Ile Asp Arg Val Asp  Asp Tyr Asp Asp Trp  Val Arg Arg 
    1085                 1090                 1095             


Phe Glu  Thr Ala Leu Thr Ala  Leu Pro Glu Lys Arg  Arg Ala Gln 
    1100                 1105                 1110             


Thr Val  Leu Pro Leu Leu His  Ala Phe Arg Ala Pro  Gln Ala Pro 
    1115                 1120                 1125             


Leu Arg  Gly Ala Pro Glu Pro  Thr Glu Val Phe His  Ala Ala Val 
    1130                 1135                 1140             


Arg Thr  Ala Lys Val Gly Pro  Gly Asp Ile Pro His  Leu Asp Glu 
    1145                 1150                 1155             


Ala Leu  Ile Asp Lys Tyr Ile  Arg Asp Leu Arg Glu  Phe Gly Leu 
    1160                 1165                 1170             


Ile 
    


<210> 4
<211> 3525
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      CAR Variant (carB12) polynucleotide"

<400> 4
atgggcacga gcgatgttca cgacgcgacc gacggcgtta ccgagactgc actgcgtgat       60

cgccagcgca ctcgtcgtat tgcagaactg tacgcaacgg acccagagtt cgcagcagca      120

gctcctctgc cggccgttgt cgatgcggcg cacaaaccgg gcctgcgtct ggcggaaatc      180

ctgcagaccc tgttcaccgg ctacggcgat cgtccggcgc tgggctatcg tgcacgtgag      240

ctggcgacgg acgaaggcgg tcgtacggtc acgcgtctgc tgccgcgctt cgataccctg      300

acctatgcac aggtgtggag ccgtgttcaa gcagtggctg cagcgttgcg tcacaatttc      360

gcacaaccga tttacccggg cgacgcggtc gcgactatcg gctttgcgag cccggactat      420

ttgacgctgg atctggtgtg cgcgtatctg ggcctggtca gcgttccttt gcagcataac      480

gctccggtgt ctcgcctggc cccgattctg gccgaggtgg aaccgcgtat tctgacggtg      540

agcgcagaat acctggacct ggcggttgaa tccgtccgtg atgtgaactc cgtcagccag      600

ctggttgttt tcgaccatca tccggaagtg gacgatcacc gtgacgcact ggctcgcgca      660

cgcgagcagc tggccggcaa aggtatcgca gttacgaccc tggatgcgat cgcagacgaa      720

ggcgcaggtt tgccggctga gccgatttac acggcggatc acgatcagcg tctggccatg      780

attctgtata ccagcggctc tacgggtgct ccgaaaggcg cgatgtacac cgaagcgatg      840

gtggctcgcc tgtggactat gagcgggatc acgggcgacc cgaccccggt tatcaacgtg      900

aacttcatgc cgctgaacca tctgggcggt cgtatcccga ttagcaccgc cgtgcagaat      960

ggcggtacca gctacttcgt tccggaaagc gacatgagca cgctgtttga ggatctggcc     1020

ctggtccgcc ctaccgaact gggtctggtg ccgcgtgttg cggacatgct gtaccagcat     1080

catctggcga ccgtggatcg cctggtgacc cagggcgcgg acgaactgac tgcggaaaag     1140

caggccggtg cggaactgcg tgaacaggtc ttgggcggtc gtgttatcac cggttttgtt     1200

tccaccgcgc cgttggcggc agagatgcgt gcttttctgg atatcacctt gggtgcacac     1260

atcgttgacg gttacggtct gaccgaaacc ggtgcggtca cccgtgatgg tgtgattgtt     1320

cgtcctccgg tcattgatta caagctgatc gatgtgccgg agctgggtta cttctccacc     1380

gacaaaccgt acccgcgtgg cgagctgctg gttcgtagca tcacgttgac tccgggttac     1440

tacaagcgcc cagaagtcac cgcgtccgtt ttcgatcgcg acggctatta ccacaccggc     1500

gacgtgatgg cagaaaccgc gccagaccac ctggtgtatg tggaccgccg caacaatgtt     1560

ctgaagctgg cgcaaggtga atttgtcgcc gtggctaacc tggagtccgt tttcagcggc     1620

gctgctctgg tccgccagat tttcgtgtat ggtaacagcg agcgcagctt tctgttggct     1680

gttgttgtcc ctaccccgga ggcgctggag caatacgacc ctgccgcatt gaaagcagcc     1740

ctggcggatt cgctgcagcg tacggcgcgt gatgccgagc tgcagagcta tgaagtgccg     1800

gcggacttca ttgttgagac tgagcctttt agcgctgcga acggtctgct gagcggtgtt     1860

ggcaagttgc tgcgtccgaa tttgaaggat cgctacggtc agcgtttgga gcagatgtac     1920

gcggacatcg cggctacgca ggcgaaccaa ttgcgtgagc tgcgtcgcgc tgcggctact     1980

caaccggtga tcgacacgct gacgcaagct gcggcgacca tcctgggtac cggcagcgag     2040

gttgcaagcg acgcacactt tactgatttg ggcggtgatt ctctgagcgc gctgacgttg     2100

agcaacttgc tgtctgactt ctttggcttt gaagtcccgg ttggcacgat tgttaaccca     2160

gcgactaatc tggcacagct ggcgcaacat atcgaggcgc agcgcacggc gggtgaccgc     2220

cgtccatcct ttacgacggt ccacggtgcg gatgctacgg aaatccgtgc aagcgaactg     2280

actctggaca aattcatcga cgctgagact ctgcgcgcag cacctggttt gccgaaggtt     2340

acgactgagc cgcgtacggt cctgttgagc ggtgccaatg gttggttggg ccgcttcctg     2400

accctgcagt ggctggaacg tttggcaccg gttggcggta ccctgatcac cattgtgcgc     2460

ggtcgtgacg atgcagcggc ctgtgcacgc ttgactcagg cttacgatac ggacccagag     2520

ctgtcccgcc gcttcgctga gttggcggat cgccacttgc gtgtggtggc aggtgatatc     2580

ggcgatcaga atctgggcct gaccccggag ctgtggcacc gtctggcagc agaggtcgat     2640

ctggtcgttc atccagcggc cctggtcaac cacgtcctgc cgtaccgcca gctgtttggt     2700

ccgaatgttg ttggcaccgc cgaagttatc aagttggctc tgaccgagcg catcaagcct     2760

gttacctacc tgtccacggc gaaggtcgcg atgggtattc ctgattttga ggaggacggt     2820

gacattcgta ccgtcagccc ggttcgtccg ctggatggtg gctatgcaaa tggctatggc     2880

aacagcaagt gggctggcga ggtgctgctg cgcgaggcac atgacctgtg tggcctgccg     2940

gttgcgacgt ttcgtagcga catgattctg gcccacccgc gctaccgtgg ccaagtgaat     3000

gtgccggaca tgttcacccg tctgctgctg tccctgctga tcacgggtgt ggcaccgcgt     3060

tccttctaca ttggtgatgg cgagcgtccg cgtgcacact acccgggcct gaccgtcgat     3120

tttgttgcgg aagcggttac taccctgggt gctcagcaac gtgagggtta tgtctcgtat     3180

gacgttatga atccgcacga tgacggtatt agcttggatg tctttgtgga ctggctgatt     3240

cgtgcgggcc acccaattga ccgtgttgac gactatgatg actgggtgcg tcgttttgaa     3300

accgcgttga ccgccttgcc ggagaaacgt cgtgcgcaga ccgttctgcc gctgctgcat     3360

gcctttcgcg cgccacaggc gccgttgcgt ggcgcccctg aaccgaccga agtgtttcat     3420

gcagcggtgc gtaccgctaa agtcggtccg ggtgatattc cgcacctgga tgaagccctg     3480

atcgacaagt acatccgtga cctgcgcgag ttcggtctga tttag                     3525


<210> 5
<211> 1173
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      CAR Variant (carB2) polypeptide"

<400> 5
Met Thr Ser Asp Val His Asp Ala Thr Asp Gly Val Thr Glu Thr Ala 
1               5                   10                  15      


Leu Asp Asp Arg Gln Ser Thr Arg Arg Ile Ala Glu Leu Tyr Ala Thr 
            20                  25                  30          


Asp Pro Glu Phe Ala Ala Ala Ala Pro Leu Pro Ala Val Val Asp Ala 
        35                  40                  45              


Ala His Lys Pro Gly Leu Arg Leu Ala Glu Ile Leu Gln Thr Leu Phe 
    50                  55                  60                  


Thr Gly Tyr Gly Asp Arg Pro Ala Leu Gly Tyr Arg Ala Arg Glu Leu 
65                  70                  75                  80  


Ala Thr Asp Glu Gly Gly Arg Thr Val Thr Arg Leu Leu Pro Arg Phe 
                85                  90                  95      


Asp Thr Leu Thr Tyr Ala Gln Val Trp Ser Arg Val Gln Ala Val Ala 
            100                 105                 110         


Ala Ala Leu Arg His Asn Phe Ala Gln Pro Ile Tyr Pro Gly Asp Ala 
        115                 120                 125             


Val Ala Thr Ile Gly Phe Ala Ser Pro Asp Tyr Leu Thr Leu Asp Leu 
    130                 135                 140                 


Val Cys Ala Tyr Leu Gly Leu Val Ser Val Pro Leu Gln His Asn Ala 
145                 150                 155                 160 


Pro Val Ser Arg Leu Ala Pro Ile Leu Ala Glu Val Glu Pro Arg Ile 
                165                 170                 175     


Leu Thr Val Ser Ala Glu Tyr Leu Asp Leu Ala Val Glu Ser Val Arg 
            180                 185                 190         


Asp Val Asn Ser Val Ser Gln Leu Val Val Phe Asp His His Pro Glu 
        195                 200                 205             


Val Asp Asp His Arg Asp Ala Leu Ala Arg Ala Arg Glu Gln Leu Ala 
    210                 215                 220                 


Gly Lys Gly Ile Ala Val Thr Thr Leu Asp Ala Ile Ala Asp Glu Gly 
225                 230                 235                 240 


Ala Gly Leu Pro Ala Glu Pro Ile Tyr Thr Ala Asp His Asp Gln Arg 
                245                 250                 255     


Leu Ala Met Ile Leu Tyr Thr Ser Gly Ser Thr Gly Ala Pro Lys Gly 
            260                 265                 270         


Ala Met Tyr Thr Glu Ala Met Val Ala Arg Leu Trp Thr Met Ser Gly 
        275                 280                 285             


Ile Thr Gly Asp Pro Thr Pro Val Ile Asn Val Asn Phe Met Pro Leu 
    290                 295                 300                 


Asn His Leu Gly Gly Arg Ile Pro Ile Ser Thr Ala Val Gln Asn Gly 
305                 310                 315                 320 


Gly Thr Ser Tyr Phe Val Pro Glu Ser Asp Met Ser Thr Leu Phe Glu 
                325                 330                 335     


Asp Leu Ala Leu Val Arg Pro Thr Glu Leu Gly Leu Val Pro Arg Val 
            340                 345                 350         


Ala Asp Met Leu Tyr Gln His His Leu Ala Thr Val Asp Arg Leu Val 
        355                 360                 365             


Thr Gln Gly Ala Asp Glu Leu Thr Ala Glu Lys Gln Ala Gly Ala Glu 
    370                 375                 380                 


Leu Arg Glu Gln Val Leu Gly Gly Arg Val Ile Thr Gly Phe Val Ser 
385                 390                 395                 400 


Thr Ala Pro Leu Ala Ala Glu Met Arg Ala Phe Leu Asp Ile Thr Leu 
                405                 410                 415     


Gly Ala His Ile Val Asp Gly Tyr Gly Leu Thr Glu Thr Gly Ala Val 
            420                 425                 430         


Thr Arg Asp Gly Val Ile Val Arg Pro Pro Val Ile Asp Tyr Lys Leu 
        435                 440                 445             


Ile Asp Val Pro Glu Leu Gly Tyr Phe Ser Thr Asp Lys Pro Tyr Pro 
    450                 455                 460                 


Arg Gly Glu Leu Leu Val Arg Ser Ile Thr Leu Thr Pro Gly Tyr Tyr 
465                 470                 475                 480 


Lys Arg Pro Glu Val Thr Ala Ser Val Phe Asp Arg Asp Gly Tyr Tyr 
                485                 490                 495     


His Thr Gly Asp Val Met Ala Glu Thr Ala Pro Asp His Leu Val Tyr 
            500                 505                 510         


Val Asp Arg Arg Asn Asn Val Leu Lys Leu Ala Gln Gly Glu Phe Val 
        515                 520                 525             


Ala Val Ala Asn Leu Glu Ser Val Phe Ser Gly Ala Ala Leu Val Arg 
    530                 535                 540                 


Gln Ile Phe Val Tyr Gly Asn Ser Glu Arg Ser Phe Leu Leu Ala Val 
545                 550                 555                 560 


Val Val Pro Thr Pro Glu Ala Leu Glu Gln Tyr Asp Pro Ala Ala Leu 
                565                 570                 575     


Lys Ala Ala Leu Ala Asp Ser Leu Gln Arg Thr Ala Arg Asp Ala Glu 
            580                 585                 590         


Leu Gln Ser Tyr Glu Val Pro Ala Asp Phe Ile Val Glu Thr Glu Pro 
        595                 600                 605             


Phe Ser Ala Ala Asn Gly Leu Leu Ser Gly Val Gly Lys Leu Leu Arg 
    610                 615                 620                 


Pro Asn Leu Lys Asp Arg Tyr Gly Gln Arg Leu Glu Gln Met Tyr Ala 
625                 630                 635                 640 


Asp Ile Ala Ala Thr Gln Ala Asn Gln Leu Arg Glu Leu Arg Arg Ala 
                645                 650                 655     


Ala Ala Thr Gln Pro Val Ile Asp Thr Leu Thr Gln Ala Ala Ala Thr 
            660                 665                 670         


Ile Leu Gly Thr Gly Ser Glu Val Ala Ser Asp Ala His Phe Thr Asp 
        675                 680                 685             


Leu Gly Gly Asp Ser Leu Ser Ala Leu Thr Leu Ser Asn Leu Leu Ser 
    690                 695                 700                 


Asp Phe Phe Gly Phe Glu Val Pro Val Gly Thr Ile Val Asn Pro Ala 
705                 710                 715                 720 


Thr Asn Leu Ala Gln Leu Ala Gln His Ile Glu Ala Gln Arg Thr Ala 
                725                 730                 735     


Gly Asp Arg Arg Pro Ser Phe Thr Thr Val His Gly Ala Asp Ala Thr 
            740                 745                 750         


Glu Ile Arg Ala Ser Glu Leu Thr Leu Asp Lys Phe Ile Asp Ala Glu 
        755                 760                 765             


Thr Leu Arg Ala Ala Pro Gly Leu Pro Lys Val Thr Thr Glu Pro Arg 
    770                 775                 780                 


Thr Val Leu Leu Ser Gly Ala Asn Gly Trp Leu Gly Arg Phe Leu Thr 
785                 790                 795                 800 


Leu Gln Trp Leu Glu Arg Leu Ala Pro Val Gly Gly Thr Leu Ile Thr 
                805                 810                 815     


Ile Val Arg Gly Arg Asp Asp Ala Ala Ala Arg Ala Arg Leu Thr Gln 
            820                 825                 830         


Ala Tyr Asp Thr Asp Pro Glu Leu Ser Arg Arg Phe Ala Glu Leu Ala 
        835                 840                 845             


Asp Arg His Leu Arg Val Val Ala Gly Asp Ile Gly Asp Pro Asn Leu 
    850                 855                 860                 


Gly Leu Thr Pro Glu Ile Trp His Arg Leu Ala Ala Glu Val Asp Leu 
865                 870                 875                 880 


Val Val His Pro Ala Ala Leu Val Asn His Val Leu Pro Tyr Arg Gln 
                885                 890                 895     


Leu Phe Gly Pro Asn Val Val Gly Thr Ala Glu Val Ile Lys Leu Ala 
            900                 905                 910         


Leu Thr Glu Arg Ile Lys Pro Val Thr Tyr Leu Ser Thr Val Ser Val 
        915                 920                 925             


Ala Met Gly Ile Pro Asp Phe Glu Glu Asp Gly Asp Ile Arg Thr Val 
    930                 935                 940                 


Ser Pro Val Arg Pro Leu Asp Gly Gly Tyr Ala Asn Gly Tyr Gly Asn 
945                 950                 955                 960 


Ser Lys Trp Ala Gly Glu Val Leu Leu Arg Glu Ala His Asp Leu Cys 
                965                 970                 975     


Gly Leu Pro Val Ala Thr Phe Arg Ser Asp Met Ile Leu Ala His Pro 
            980                 985                 990         


Arg Tyr Arg Gly Gln Val Asn Val  Pro Asp Met Phe Thr  Arg Leu Leu 
        995                 1000                 1005             


Leu Ser  Leu Leu Ile Thr Gly  Val Ala Pro Arg Ser  Phe Tyr Ile 
    1010                 1015                 1020             


Gly Asp  Gly Glu Arg Pro Arg  Ala His Tyr Pro Gly  Leu Thr Val 
    1025                 1030                 1035             


Asp Phe  Val Ala Glu Ala Val  Thr Thr Leu Gly Ala  Gln Gln Arg 
    1040                 1045                 1050             


Glu Gly  Tyr Val Ser Tyr Asp  Val Met Asn Pro His  Asp Asp Gly 
    1055                 1060                 1065             


Ile Ser  Leu Asp Val Phe Val  Asp Trp Leu Ile Arg  Ala Gly His 
    1070                 1075                 1080             


Pro Ile  Asp Arg Val Asp Asp  Tyr Asp Asp Trp Val  Arg Arg Phe 
    1085                 1090                 1095             


Glu Thr  Ala Leu Thr Ala Leu  Pro Glu Lys Arg Arg  Ala Gln Thr 
    1100                 1105                 1110             


Val Leu  Pro Leu Leu His Ala  Phe Arg Ala Pro Gln  Ala Pro Leu 
    1115                 1120                 1125             


Arg Gly  Ala Pro Glu Pro Thr  Glu Val Phe His Ala  Ala Val Arg 
    1130                 1135                 1140             


Thr Ala  Lys Val Gly Pro Gly  Asp Ile Pro His Leu  Asp Glu Ala 
    1145                 1150                 1155             


Leu Ile  Asp Lys Tyr Ile Arg  Asp Leu Arg Glu Phe  Gly Leu Ile 
    1160                 1165                 1170             


<210> 6
<211> 3525
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      CAR Variant (carB2) polynucleotide"

<400> 6
atgggcacga gcgatgttca cgacgcgacc gacggcgtta ccgagactgc actggatgat       60

cgccagagca ctcgtcgtat tgcagaactg tacgcaacgg acccagagtt cgcagcagca      120

gctcctctgc cggccgttgt cgatgcggcg cacaaaccgg gcctgcgtct ggcggaaatc      180

ctgcagaccc tgttcaccgg ctacggcgat cgtccggcgc tgggctatcg tgcacgtgag      240

ctggcgacgg acgaaggcgg tcgtacggtc acgcgtctgc tgccgcgctt cgataccctg      300

acctatgcac aggtgtggag ccgtgttcaa gcagtggctg cagcgttgcg tcacaatttc      360

gcacaaccga tttacccggg cgacgcggtc gcgactatcg gctttgcgag cccggactat      420

ttgacgctgg atctggtgtg cgcgtatctg ggcctggtca gcgttccttt gcagcataac      480

gctccggtgt ctcgcctggc cccgattctg gccgaggtgg aaccgcgtat tctgacggtg      540

agcgcagaat acctggacct ggcggttgaa tccgtccgtg atgtgaactc cgtcagccag      600

ctggttgttt tcgaccatca tccggaagtg gacgatcacc gtgacgcact ggctcgcgca      660

cgcgagcagc tggccggcaa aggtatcgca gttacgaccc tggatgcgat cgcagacgaa      720

ggcgcaggtt tgccggctga gccgatttac acggcggatc acgatcagcg tctggccatg      780

attctgtata ccagcggctc tacgggtgct ccgaaaggcg cgatgtacac cgaagcgatg      840

gtggctcgcc tgtggactat gagcgggatc acgggcgacc cgaccccggt tatcaacgtg      900

aacttcatgc cgctgaacca tctgggcggt cgtatcccga ttagcaccgc cgtgcagaat      960

ggcggtacca gctacttcgt tccggaaagc gacatgagca cgctgtttga ggatctggcc     1020

ctggtccgcc ctaccgaact gggtctggtg ccgcgtgttg cggacatgct gtaccagcat     1080

catctggcga ccgtggatcg cctggtgacc cagggcgcgg acgaactgac tgcggaaaag     1140

caggccggtg cggaactgcg tgaacaggtc ttgggcggtc gtgttatcac cggttttgtt     1200

tccaccgcgc cgttggcggc agagatgcgt gcttttctgg atatcacctt gggtgcacac     1260

atcgttgacg gttacggtct gaccgaaacc ggtgcggtca cccgtgatgg tgtgattgtt     1320

cgtcctccgg tcattgatta caagctgatc gatgtgccgg agctgggtta cttctccacc     1380

gacaaaccgt acccgcgtgg cgagctgctg gttcgtagca tcacgttgac tccgggttac     1440

tacaagcgcc cagaagtcac cgcgtccgtt ttcgatcgcg acggctatta ccacaccggc     1500

gacgtgatgg cagaaaccgc gccagaccac ctggtgtatg tggaccgccg caacaatgtt     1560

ctgaagctgg cgcaaggtga atttgtcgcc gtggctaacc tggagtccgt tttcagcggc     1620

gctgctctgg tccgccagat tttcgtgtat ggtaacagcg agcgcagctt tctgttggct     1680

gttgttgtcc ctaccccgga ggcgctggag caatacgacc ctgccgcatt gaaagcagcc     1740

ctggcggatt cgctgcagcg tacggcgcgt gatgccgagc tgcagagcta tgaagtgccg     1800

gcggacttca ttgttgagac tgagcctttt agcgctgcga acggtctgct gagcggtgtt     1860

ggcaagttgc tgcgtccgaa tttgaaggat cgctacggtc agcgtttgga gcagatgtac     1920

gcggacatcg cggctacgca ggcgaaccaa ttgcgtgagc tgcgtcgcgc tgcggctact     1980

caaccggtga tcgacacgct gacgcaagct gcggcgacca tcctgggtac cggcagcgag     2040

gttgcaagcg acgcacactt tactgatttg ggcggtgatt ctctgagcgc gctgacgttg     2100

agcaacttgc tgtctgactt ctttggcttt gaagtcccgg ttggcacgat tgttaaccca     2160

gcgactaatc tggcacagct ggcgcaacat atcgaggcgc agcgcacggc gggtgaccgc     2220

cgtccatcct ttacgacggt ccacggtgcg gatgctacgg aaatccgtgc aagcgaactg     2280

actctggaca aattcatcga cgctgagact ctgcgcgcag cacctggttt gccgaaggtt     2340

acgactgagc cgcgtacggt cctgttgagc ggtgccaatg gttggttggg ccgcttcctg     2400

accctgcagt ggctggaacg tttggcaccg gttggcggta ccctgatcac cattgtgcgc     2460

ggtcgtgacg atgcagcggc ccgcgcacgc ttgactcagg cttacgatac ggacccagag     2520

ctgtcccgcc gcttcgctga gttggcggat cgccacttgc gtgtggtggc aggtgatatc     2580

ggcgatccga atctgggcct gaccccggag atttggcacc gtctggcagc agaggtcgat     2640

ctggtcgttc atccagcggc cctggtcaac cacgtcctgc cgtaccgcca gctgtttggt     2700

ccgaatgttg ttggcaccgc cgaagttatc aagttggctc tgaccgagcg catcaagcct     2760

gttacctacc tgtccacggt tagcgtcgcg atgggtattc ctgattttga ggaggacggt     2820

gacattcgta ccgtcagccc ggttcgtccg ctggatggtg gctatgcaaa tggctatggc     2880

aacagcaagt gggctggcga ggtgctgctg cgcgaggcac atgacctgtg tggcctgccg     2940

gttgcgacgt ttcgtagcga catgattctg gcccacccgc gctaccgtgg ccaagtgaat     3000

gtgccggaca tgttcacccg tctgctgctg tccctgctga tcacgggtgt ggcaccgcgt     3060

tccttctaca ttggtgatgg cgagcgtccg cgtgcacact acccgggcct gaccgtcgat     3120

tttgttgcgg aagcggttac taccctgggt gctcagcaac gtgagggtta tgtctcgtat     3180

gacgttatga atccgcacga tgacggtatt agcttggatg tctttgtgga ctggctgatt     3240

cgtgcgggcc acccaattga ccgtgttgac gactatgatg actgggtgcg tcgttttgaa     3300

accgcgttga ccgccttgcc ggagaaacgt cgtgcgcaga ccgttctgcc gctgctgcat     3360

gcctttcgcg cgccacaggc gccgttgcgt ggcgcccctg aaccgaccga agtgtttcat     3420

gcagcggtgc gtaccgctaa agtcggtccg ggtgatattc cgcacctgga tgaagccctg     3480

atcgacaagt acatccgtga cctgcgcgag ttcggtctga tttag                     3525


<210> 7
<211> 1174
<212> PRT
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      CAR Variant (carB8) polypeptide"

<400> 7
Met Gly Thr Ser Asp Val His Asp Ala Thr Asp Gly Val Thr Glu Thr 
1               5                   10                  15      


Ala Leu Asp Asp Arg Gln Arg Thr Arg Arg Ile Ala Glu Leu Tyr Ala 
            20                  25                  30          


Thr Asp Pro Glu Phe Ala Ala Ala Ala Pro Leu Pro Ala Val Val Asp 
        35                  40                  45              


Ala Ala His Lys Pro Gly Leu Arg Leu Ala Glu Ile Leu Gln Thr Leu 
    50                  55                  60                  


Phe Thr Gly Tyr Gly Asp Arg Pro Ala Leu Gly Tyr Arg Ala Arg Glu 
65                  70                  75                  80  


Leu Ala Thr Asp Glu Gly Gly Arg Thr Val Thr Arg Leu Leu Pro Arg 
                85                  90                  95      


Phe Asp Thr Leu Thr Tyr Ala Gln Val Trp Ser Arg Val Gln Ala Val 
            100                 105                 110         


Ala Ala Ala Leu Arg His Asn Phe Ala Gln Pro Ile Tyr Pro Gly Asp 
        115                 120                 125             


Ala Val Ala Thr Ile Gly Phe Ala Ser Pro Asp Tyr Leu Thr Leu Asp 
    130                 135                 140                 


Leu Val Cys Ala Tyr Leu Gly Leu Val Ser Val Pro Leu Gln His Asn 
145                 150                 155                 160 


Ala Pro Val Ser Arg Leu Ala Pro Ile Leu Ala Glu Val Glu Pro Arg 
                165                 170                 175     


Ile Leu Thr Val Ser Ala Glu Tyr Leu Asp Leu Ala Val Glu Ser Val 
            180                 185                 190         


Arg Asp Val Asn Ser Val Ser Gln Leu Val Val Phe Asp His His Pro 
        195                 200                 205             


Glu Val Asp Asp His Arg Asp Ala Leu Ala Arg Ala Arg Glu Gln Leu 
    210                 215                 220                 


Ala Gly Lys Gly Ile Ala Val Thr Thr Leu Asp Ala Ile Ala Asp Glu 
225                 230                 235                 240 


Gly Ala Gly Leu Pro Ala Glu Pro Ile Tyr Thr Ala Asp His Asp Gln 
                245                 250                 255     


Arg Leu Ala Met Ile Leu Tyr Thr Ser Gly Ser Thr Gly Ala Pro Lys 
            260                 265                 270         


Gly Ala Met Tyr Thr Glu Ala Met Val Ala Arg Leu Trp Thr Met Ser 
        275                 280                 285             


Gly Ile Thr Gly Asp Pro Thr Pro Val Ile Asn Val Asn Phe Met Pro 
    290                 295                 300                 


Leu Asn His Leu Gly Gly Arg Ile Pro Ile Ser Thr Ala Val Gln Asn 
305                 310                 315                 320 


Gly Gly Thr Ser Tyr Phe Val Pro Glu Ser Asp Met Ser Thr Leu Phe 
                325                 330                 335     


Glu Asp Leu Ala Leu Val Arg Pro Thr Glu Leu Gly Leu Val Pro Arg 
            340                 345                 350         


Val Ala Asp Met Leu Tyr Gln His His Leu Ala Thr Val Asp Arg Leu 
        355                 360                 365             


Val Thr Gln Gly Ala Asp Glu Leu Thr Ala Glu Lys Gln Ala Gly Ala 
    370                 375                 380                 


Glu Leu Arg Glu Gln Val Leu Gly Gly Arg Val Ile Thr Gly Phe Val 
385                 390                 395                 400 


Ser Thr Ala Pro Leu Ala Ala Glu Met Arg Ala Phe Leu Asp Ile Thr 
                405                 410                 415     


Leu Gly Ala His Ile Val Asp Gly Tyr Gly Leu Thr Glu Thr Gly Ala 
            420                 425                 430         


Val Thr Arg Asp Gly Val Ile Val Arg Pro Pro Val Ile Asp Tyr Lys 
        435                 440                 445             


Leu Ile Asp Val Pro Glu Leu Gly Tyr Phe Ser Thr Asp Lys Pro Tyr 
    450                 455                 460                 


Pro Arg Gly Glu Leu Leu Val Arg Ser His Thr Leu Thr Pro Gly Tyr 
465                 470                 475                 480 


Tyr Lys Arg Pro Glu Val Thr Ala Ser Val Phe Asp Arg Asp Gly Tyr 
                485                 490                 495     


Tyr His Thr Gly Asp Val Met Ala Glu Thr Ala Pro Asp His Leu Val 
            500                 505                 510         


Tyr Val Asp Arg Arg Asn Asn Val Leu Lys Leu Ala Gln Gly Glu Phe 
        515                 520                 525             


Val Ala Val Ala Asn Leu Glu Ser Val Phe Ser Gly Ala Ala Leu Val 
    530                 535                 540                 


Arg Gln Ile Phe Val Tyr Gly Asn Ser Glu Arg Ser Phe Leu Leu Ala 
545                 550                 555                 560 


Val Val Val Pro Thr Pro Glu Ala Leu Glu Gln Tyr Asp Pro Ala Ala 
                565                 570                 575     


Leu Lys Ala Ala Leu Ala Asp Ser Leu Gln Arg Thr Ala Arg Asp Ala 
            580                 585                 590         


Glu Leu Gln Ser Tyr Glu Val Pro Ala Asp Phe Ile Val Glu Thr Glu 
        595                 600                 605             


Pro Phe Ser Ala Ala Asn Gly Leu Leu Ser Gly Val Gly Lys Leu Leu 
    610                 615                 620                 


Arg Pro Asn Leu Lys Asp Arg Tyr Gly Gln Arg Leu Glu Gln Met Tyr 
625                 630                 635                 640 


Ala Asp Ile Ala Ala Thr Gln Ala Asn Gln Leu Arg Glu Leu Arg Arg 
                645                 650                 655     


Ala Ala Ala Thr Gln Pro Val Ile Asp Thr Leu Thr Gln Ala Ala Ala 
            660                 665                 670         


Thr Ile Leu Gly Thr Gly Ser Glu Val Ala Ser Asp Ala His Phe Thr 
        675                 680                 685             


Asp Leu Gly Gly Asp Ser Leu Ser Ala Leu Thr Leu Ser Asn Leu Leu 
    690                 695                 700                 


Ser Asp Phe Phe Gly Phe Glu Val Pro Val Gly Thr Ile Val Asn Pro 
705                 710                 715                 720 


Ala Thr Asn Leu Ala Gln Leu Ala Gln His Ile Glu Ala Gln Arg Thr 
                725                 730                 735     


Ala Gly Asp Arg Arg Pro Ser Phe Thr Thr Val His Gly Ala Asp Ala 
            740                 745                 750         


Thr Glu Ile Arg Ala Ser Glu Leu Thr Leu Asp Lys Phe Ile Asp Ala 
        755                 760                 765             


Glu Thr Leu Arg Ala Ala Pro Gly Leu Pro Lys Val Thr Thr Glu Pro 
    770                 775                 780                 


Arg Thr Val Leu Leu Ser Gly Ala Asn Gly Trp Leu Gly Arg Phe Leu 
785                 790                 795                 800 


Thr Leu Gln Trp Leu Glu Arg Leu Ala Pro Val Gly Gly Thr Leu Ile 
                805                 810                 815     


Thr Ile Val Arg Gly Arg Asp Asp Ala Ala Ala Arg Ala Arg Leu Thr 
            820                 825                 830         


Gln Ala Tyr Asp Thr Asp Pro Glu Leu Ser Arg Arg Phe Ala Glu Leu 
        835                 840                 845             


Ala Asp Arg His Leu Arg Val Val Ala Gly Asp Ile Gly Asp Pro Asn 
    850                 855                 860                 


Leu Gly Leu Thr Pro Glu Ile Trp His Ser Leu Ala Ala Glu Val Asp 
865                 870                 875                 880 


Leu Val Val His Pro Ala Ala Leu Val Asn His Val Leu Pro Tyr Arg 
                885                 890                 895     


Gln Leu Phe Gly Pro Asn Val Val Gly Thr Ala Glu Val Ile Lys Leu 
            900                 905                 910         


Ala Leu Thr Glu Arg Ile Lys Pro Val Thr Tyr Leu Ser Thr Val Gly 
        915                 920                 925             


Val Ala Arg Gly Ile Pro Asp Phe Glu Glu Asp Gly Asp Ile Arg Thr 
    930                 935                 940                 


Val Ser Pro Val Arg Pro Leu Asp Gly Gly Tyr Ala Asn Gly Tyr Gly 
945                 950                 955                 960 


Asn Ser Lys Trp Ala Gly Glu Val Leu Leu Arg Glu Ala His Asp Leu 
                965                 970                 975     


Cys Gly Leu Pro Val Ala Thr Phe Arg Ser Asp Met Ile Leu Ala His 
            980                 985                 990         


Pro Arg Tyr Arg Gly Gln Val Asn  Val Pro Asp Met Phe  Thr Arg Leu 
        995                 1000                 1005             


Leu Leu  Ser Leu Leu Ile Thr  Gly Val Ala Pro Arg  Ser Phe Tyr 
    1010                 1015                 1020             


Ile Gly  Asp Gly Glu Arg Pro  Arg Ala His Tyr Pro  Gly Leu Thr 
    1025                 1030                 1035             


Val Asp  Phe Val Ala Glu Ala  Val Thr Thr Leu Gly  Ala Gln Gln 
    1040                 1045                 1050             


Arg Glu  Gly Tyr Val Ser Tyr  Asp Val Met Asn Pro  His Asp Asp 
    1055                 1060                 1065             


Gly Ile  Ser Leu Asp Val Phe  Val Asp Trp Leu Ile  Arg Ala Gly 
    1070                 1075                 1080             


His Pro  Ile Asp Arg Val Asp  Asp Tyr Asp Asp Trp  Val Arg Arg 
    1085                 1090                 1095             


Phe Glu  Thr Ala Leu Thr Ala  Leu Pro Glu Lys Arg  Arg Ala Gln 
    1100                 1105                 1110             


Thr Val  Leu Pro Leu Leu His  Ala Phe Arg Ala Pro  Gln Ala Pro 
    1115                 1120                 1125             


Trp Arg  Gly Ala Pro Glu Pro  Thr Glu Val Phe His  Ala Ala Val 
    1130                 1135                 1140             


Arg Thr  Ala Lys Val Gly Pro  Gly Asp Ile Pro His  Leu Asp Glu 
    1145                 1150                 1155             


Ala Leu  Ile Asp Lys Tyr Ile  Arg Asp Leu Arg Glu  Phe Gly Leu 
    1160                 1165                 1170             


Ile 
    


<210> 8
<211> 3525
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      CAR Variant (carB8) polynucleotide"

<400> 8
atgggcacga gcgatgttca cgacgcgacc gacggcgtta ccgagactgc actggatgat       60

cgccagagga ctcgtcgtat tgcagaactg tacgcaacgg acccagagtt cgcagcagca      120

gctcctctgc cggccgttgt cgatgcggcg cacaaaccgg gcctgcgtct ggcggaaatc      180

ctgcagaccc tgttcaccgg ctacggcgat cgtccggcgc tgggctatcg tgcacgtgag      240

ctggcgacgg acgaaggcgg tcgtacggtc acgcgtctgc tgccgcgctt cgataccctg      300

acctatgcac aggtgtggag ccgtgttcaa gcagtggctg cagcgttgcg tcacaatttc      360

gcacaaccga tttacccggg cgacgcggtc gcgactatcg gctttgcgag cccggactat      420

ttgacgctgg atctggtgtg cgcgtatctg ggcctggtca gcgttccttt gcagcataac      480

gctccggtgt ctcgcctggc cccgattctg gccgaggtgg aaccgcgtat tctgacggtg      540

agcgcagaat acctggacct ggcggttgaa tccgtccgtg atgtgaactc cgtcagccag      600

ctggttgttt tcgaccatca tccggaagtg gacgatcacc gtgacgcact ggctcgcgca      660

cgcgagcagc tggccggcaa aggtatcgca gttacgaccc tggatgcgat cgcagacgaa      720

ggcgcaggtt tgccggctga gccgatttac acggcggatc acgatcagcg tctggccatg      780

attctgtata ccagcggctc tacgggtgct ccgaaaggcg cgatgtacac cgaagcgatg      840

gtggctcgcc tgtggactat gagcgggatc acgggcgacc cgaccccggt tatcaacgtg      900

aacttcatgc cgctgaacca tctgggcggt cgtatcccga ttagcaccgc cgtgcagaat      960

ggcggtacca gctacttcgt tccggaaagc gacatgagca cgctgtttga ggatctggcc     1020

ctggtccgcc ctaccgaact gggtctggtg ccgcgtgttg cggacatgct gtaccagcat     1080

catctggcga ccgtggatcg cctggtgacc cagggcgcgg acgaactgac tgcggaaaag     1140

caggccggtg cggaactgcg tgaacaggtc ttgggcggtc gtgttatcac cggttttgtt     1200

tccaccgcgc cgttggcggc agagatgcgt gcttttctgg atatcacctt gggtgcacac     1260

atcgttgacg gttacggtct gaccgaaacc ggtgcggtca cccgtgatgg tgtgattgtt     1320

cgtcctccgg tcattgatta caagctgatc gatgtgccgg agctgggtta cttctccacc     1380

gacaaaccgt acccgcgtgg cgagctgctg gttcgtagcc acacgttgac tccgggttac     1440

tacaagcgcc cagaagtcac cgcgtccgtt ttcgatcgcg acggctatta ccacaccggc     1500

gacgtgatgg cagaaaccgc gccagaccac ctggtgtatg tggaccgccg caacaatgtt     1560

ctgaagctgg cgcaaggtga atttgtcgcc gtggctaacc tggagtccgt tttcagcggc     1620

gctgctctgg tccgccagat tttcgtgtat ggtaacagcg agcgcagctt tctgttggct     1680

gttgttgtcc ctaccccgga ggcgctggag caatacgacc ctgccgcatt gaaagcagcc     1740

ctggcggatt cgctgcagcg tacggcgcgt gatgccgagc tgcagagcta tgaagtgccg     1800

gcggacttca ttgttgagac tgagcctttt agcgctgcga acggtctgct gagcggtgtt     1860

ggcaagttgc tgcgtccgaa tttgaaggat cgctacggtc agcgtttgga gcagatgtac     1920

gcggacatcg cggctacgca ggcgaaccaa ttgcgtgagc tgcgtcgcgc tgcggctact     1980

caaccggtga tcgacacgct gacgcaagct gcggcgacca tcctgggtac cggcagcgag     2040

gttgcaagcg acgcacactt tactgatttg ggcggtgatt ctctgagcgc gctgacgttg     2100

agcaacttgc tgtctgactt ctttggcttt gaagtcccgg ttggcacgat tgttaaccca     2160

gcgactaatc tggcacagct ggcgcaacat atcgaggcgc agcgcacggc gggtgaccgc     2220

cgtccatcct ttacgacggt ccacggtgcg gatgctacgg aaatccgtgc aagcgaactg     2280

actctggaca aattcatcga cgctgagact ctgcgcgcag cacctggttt gccgaaggtt     2340

acgactgagc cgcgtacggt cctgttgagc ggtgccaatg gttggttggg ccgcttcctg     2400

accctgcagt ggctggaacg tttggcaccg gttggcggta ccctgatcac cattgtgcgc     2460

ggtcgtgacg atgcagcggc ccgcgcacgc ttgactcagg cttacgatac ggacccagag     2520

ctgtcccgcc gcttcgctga gttggcggat cgccacttgc gtgtggtggc aggtgatatc     2580

ggcgatccga atctgggcct gaccccggag atttggcaca gtctggcagc agaggtcgat     2640

ctggtcgttc atccagcggc cctggtcaac cacgtcctgc cgtaccgcca gctgtttggt     2700

ccgaatgttg ttggcaccgc cgaagttatc aagttggctc tgaccgagcg catcaagcct     2760

gttacctacc tgtccacggt tggggtcgcg aggggtattc ctgattttga ggaggacggt     2820

gacattcgta ccgtcagccc ggttcgtccg ctggatggtg gctatgcaaa tggctatggc     2880

aacagcaagt gggctggcga ggtgctgctg cgcgaggcac atgacctgtg tggcctgccg     2940

gttgcgacgt ttcgtagcga catgattctg gcccacccgc gctaccgtgg ccaagtgaat     3000

gtgccggaca tgttcacccg tctgctgctg tccctgctga tcacgggtgt ggcaccgcgt     3060

tccttctaca ttggtgatgg cgagcgtccg cgtgcacact acccgggcct gaccgtcgat     3120

tttgttgcgg aagcggttac taccctgggt gctcagcaac gtgagggtta tgtctcgtat     3180

gacgttatga atccgcacga tgacggtatt agcttggatg tctttgtgga ctggctgatt     3240

cgtgcgggcc acccaattga ccgtgttgac gactatgatg actgggtgcg tcgttttgaa     3300

accgcgttga ccgccttgcc ggagaaacgt cgtgcgcaga ccgttctgcc gctgctgcat     3360

gcctttcgcg cgccacaggc gccgtggcgt ggcgcccctg aaccgaccga agtgtttcat     3420

gcagcggtgc gtaccgctaa agtcggtccg ggtgatattc cgcacctgga tgaagccctg     3480

atcgacaagt acatccgtga cctgcgcgag ttcggtctga tttag                     3525
