                         SEQUENCE LISTING

<110>  NORTHWESTERN UNIVERSITY
 
<120>  YEAST-BASED BIOSENSOR

<130>  NWEST-34018/WO-1/ORD

<150>  US 62/023,549
<151>  2014-07-11

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  13
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  1

Trp His Trp Leu Gln Leu Lys Pro Gly Gln Pro Met Tyr 
1               5                   10              


<210>  2
<211>  13
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  2

Trp Ala Trp Leu Gln Leu Lys Pro Gly Gln Pro Met Tyr 
1               5                   10              


<210>  3
<211>  13
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  3

Trp His Tyr Leu Gln Leu Lys Pro Gly Gln Pro Met Tyr 
1               5                   10              


<210>  4
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Cystatin chimera

<400>  4

His Ala Leu Ala Leu Lys Pro Gly Glu Pro Met Tyr 
1               5                   10          


<210>  5
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Cystatin chimera

<400>  5

Ala Leu Ala Leu Lys Pro Gly Glu Pro Met Tyr 
1               5                   10      


<210>  6
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  GSS chimera

<400>  6

Trp His Trp Leu Gln Leu Lys Pro Gly Glu Pro Leu Tyr Gly Arg 
1               5                   10                  15  


<210>  7
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Cystatin chimera

<400>  7

Ala Leu Asp Phe Lys Pro Gly Glu Pro Met Tyr 
1               5                   10      


<210>  8
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Cystatin chimera

<400>  8

Ala Leu Asp Leu Ala Val Gly Glu Pro Met Tyr 
1               5                   10      


<210>  9
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Cystatin chimera

<400>  9

Ala Leu Asp Phe Ala Val Gly Glu Tyr Asn Lys 
1               5                   10      


<210>  10
<211>  13
<212>  PRT
<213>  Artificial sequence

<220>
<223>  GSS chimera

<400>  10

Leu His Leu Leu Ala Leu Lys Pro Gly Gln Pro Met Tyr 
1               5                   10              


<210>  11
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  GSS chimera

<400>  11

Leu His Leu Leu Ala Leu Lys Pro Gly Gln Pro Leu Tyr Gly Arg 
1               5                   10                  15  


<210>  12
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  GSS chimera

<400>  12

Leu His Leu Leu Ala Gly Gln Pro Gly Glu Ser Leu Tyr Gly Arg 
1               5                   10                  15  


<210>  13
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  GSS chimera

<400>  13

Leu Leu Leu Leu Ala Gly Gln Pro Glu Glu Ser Leu Ala Gly Arg 
1               5                   10                  15  


<210>  14
<211>  431
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  14

Met Ser Asp Ala Ala Pro Ser Leu Ser Asn Leu Phe Tyr Asp Pro Thr 
1               5                   10                  15      


Tyr Asn Pro Gly Gln Ser Thr Ile Asn Tyr Thr Ser Ile Tyr Gly Asn 
            20                  25                  30          


Gly Thr Thr Ile Thr Phe Asp Glu Leu Gln Gly Leu Val Asn Ser Thr 
        35                  40                  45              


Val Thr Gln Ala Ile Met Phe Gly Val Arg Cys Gly Ala Ala Ala Leu 
    50                  55                  60                  


Thr Leu Ile Val Met Trp Met Thr Ser Arg Ser Arg Lys Thr Pro Ile 
65                  70                  75                  80  


Phe Ile Ile Asn Gln Val Ser Leu Phe Leu Ile Ile Leu His Ser Ala 
                85                  90                  95      


Leu Tyr Phe Lys Tyr Leu Leu Ser Asn Tyr Ser Ser Val Thr Tyr Ala 
            100                 105                 110         


Leu Thr Gly Phe Pro Gln Phe Ile Ser Arg Gly Asp Val His Val Tyr 
        115                 120                 125             


Gly Ala Thr Asn Ile Ile Gln Val Leu Leu Val Ala Ser Ile Glu Thr 
    130                 135                 140                 


Ser Leu Val Phe Gln Ile Lys Val Ile Phe Thr Gly Asp Asn Phe Lys 
145                 150                 155                 160 


Arg Ile Gly Leu Met Leu Thr Ser Ile Ser Phe Thr Leu Gly Ile Thr 
                165                 170                 175     


Thr Val Thr Met Tyr Phe Val Ser Ala Val Lys Gly Met Ile Val Thr 
            180                 185                 190         


Tyr Asn Asp Val Ser Ala Thr Gln Gly Lys Tyr Phe Asn Ala Ser Thr 
        195                 200                 205             


Ile Leu Leu Ala Ser Ser Ile Asn Phe Met Ser Phe Val Leu Val Val 
    210                 215                 220                 


Lys Leu Ile Leu Ala Ile Arg Ser Arg Arg Phe Leu Gly Leu Lys Gln 
225                 230                 235                 240 


Phe Asp Ser Phe His Ile Leu Leu Ile Met Ser Cys Gln Ser Leu Leu 
                245                 250                 255     


Val Pro Ser Ile Ile Phe Ile Leu Ala Tyr Ser Leu Lys Pro Asn Gln 
            260                 265                 270         


Gly Thr Asp Val Leu Thr Thr Val Ala Thr Leu Leu Ala Val Leu Ser 
        275                 280                 285             


Leu Pro Leu Ser Ser Met Trp Ala Thr Ala Ala Asn Asn Ala Ser Lys 
    290                 295                 300                 


Thr Asn Thr Ile Thr Ser Asp Phe Thr Thr Ser Thr Asp Arg Phe Tyr 
305                 310                 315                 320 


Pro Gly Thr Leu Ser Ser Phe Gln Thr Asp Ser Ile Asn Asn Asp Ala 
                325                 330                 335     


Lys Ser Ser Leu Arg Ser Arg Leu Tyr Asp Leu Tyr Pro Arg Arg Lys 
            340                 345                 350         


Glu Thr Thr Ser Asp Lys His Ser Glu Arg Thr Phe Val Ser Glu Thr 
        355                 360                 365             


Ala Asn Asp Ile Glu Lys Asn Gln Phe Tyr Gln Leu Pro Thr Pro Thr 
    370                 375                 380                 


Ser Ser Lys Asn Thr Arg Ile Gly Pro Phe Ala Asp Ala Ser Tyr Lys 
385                 390                 395                 400 


Glu Gly Glu Val Glu Pro Val Asp Met Tyr Thr Pro Asp Thr Ala Ala 
                405                 410                 415     


Asp Glu Glu Ala Arg Lys Phe Trp Thr Glu Asp Asn Asn Asn Leu 
            420                 425                 430     


<210>  15
<211>  7067
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Synthetic

<400>  15
aattcgatat caagcttatc gataccgtcg acctcgagtc atgtaattag ttatgtcacg       60

cttacattca cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc      120

tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat      180

atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa      240

aaccttgctt gagaaggttt tgggacgctc gaaggcttta atttgcggcc ggtacccaat      300

tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac      360

tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc      420

tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat      480

ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg      540

cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct      600

tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta      660

gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt      720

tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg      780

ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat      840

tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt      900

taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc ctgatgcggt      960

attttctcct tacgcatctg tgcggtattt cacaccgcat agggtaataa ctgatataat     1020

taaattgaag ctctaatttg tgagtttagt atacatgcat ttacttataa tacagttttt     1080

tagttttgct ggccgcatct tctcaaatat gcttcccagc ctgcttttct gtaacgttca     1140

ccctctacct tagcatccct tccctttgca aatagtcctc ttccaacaat aataatgtca     1200

gatcctgtag agaccacatc atccacggtt ctatactgtt gacccaatgc gtctcccttg     1260

tcatctaaac ccacaccggg tgtcataatc aaccaatcgt aaccttcatc tcttccaccc     1320

atgtctcttt gagcaataaa gccgataaca aaatctttgt cgctcttcgc aatgtcaaca     1380

gtacccttag tatattctcc agtagatagg gagcccttgc atgacaattc tgctaacatc     1440

aaaaggcctc taggttcctt tgttacttct tctgccgcct gcttcaaacc gctaacaata     1500

cctgggccca ccacaccgtg tgcattcgta atgtctgccc attctgctat tctgtataca     1560

cccgcagagt actgcaattt gactgtatta ccaatgtcag caaattttct gtcttcgaag     1620

agtaaaaaat tgtacttggc ggataatgcc tttagcggct taactgtgcc ctccatggaa     1680

aaatcagtca agatatccac atgtgttttt agtaaacaaa ttttgggacc taatgcttca     1740

actaactcca gtaattcctt ggtggtacga acatccaatg aagcacacaa gtttgtttgc     1800

ttttcgtgca tgatattaaa tagcttggca gcaacaggac taggatgagt agcagcacgt     1860

tccttatatg tagctttcga catgatttat cttcgtttcc tgcaggtttt tgttctgtgc     1920

agttgggtta agaatactgg gcaatttcat gtttcttcaa cactacatat gcgtatatat     1980

accaatctaa gtctgtgctc cttccttcgt tcttccttct gttcggagat taccgaatca     2040

aaaaaatttc aaagaaaccg aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaat     2100

tgaaaagctg tggtatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc     2160

cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca     2220

tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg     2280

tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat     2340

gtcatgataa taatggtttc ttaggacgga tcgcttgcct gtaacttaca cgcgcctcgt     2400

atcttttaat gatggaataa tttgggaatt tactctgtgt ttatttattt ttatgttttg     2460

tatttggatt ttagaaagta aataaagaag gtagaagagt tacggaatga agaaaaaaaa     2520

ataaacaaag gtttaaaaaa tttcaacaaa aagcgtactt tacatatata tttattagac     2580

aagaaaagca gattaaatag atatacattc gattaacgat aagtaaaatg taaaatcaca     2640

ggattttcgt gtgtggtctt ctacacagac aagatgaaac aattcggcat taatacctga     2700

gagcaggaag agcaagataa aaggtagtat ttgttggcga tccccctaga gtcttttaca     2760

tcttcggaaa acaaaaacta ttttttcttt aatttctttt tttactttct atttttaatt     2820

tatatattta tattaaaaaa tttaaattat aattattttt atagcacgtg atgaaaagga     2880

cccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat     2940

acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg     3000

aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc     3060

attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga     3120

tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga     3180

gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg     3240

cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc     3300

tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac     3360

agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact     3420

tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca     3480

tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg     3540

tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact     3600

acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg     3660

accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg     3720

tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat     3780

cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc     3840

tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat     3900

actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt     3960

tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc     4020

cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt     4080

gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac     4140

tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt     4200

gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct     4260

gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga     4320

ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac     4380

acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg     4440

agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt     4500

cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc     4560

tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg     4620

gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc     4680

ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc     4740

ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag     4800

cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca     4860

ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat     4920

taatgtgagt tacctcactc attaggcacc ccaggcttta cactttatgc ttccggctcc     4980

tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga     5040

ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tcagtttatc     5100

attatcaata ctcgccattt caaagaatac gtaaataatt aatagtagtg attttcctaa     5160

ctttatttag tcaaaaaatt agccttttaa ttctgctgta acccgtacat gcccaaaata     5220

gggggcgggt tacacagaat atataacatc gtaggtgtct gggtgaacag tttattcctg     5280

gcatccacta aatataatgg agcccgcttt ttaagctggc atccagaaaa aaaaagaatc     5340

ccagcaccaa aatattgttt tcttcaccaa ccatcagttc ataggtccat tctcttagcg     5400

caactacaga gaacaggggc acaaacaggc aaaaaacggg cacaacctca atggagtgat     5460

gcaacctgcc tggagtaaat gatgacacaa ggcaattgac ccacgcatgt atctatctca     5520

ttttcttaca ccttctatta ccttctgctc tctctgattt ggaaaaagct gaaaaaaaag     5580

gttgaaacca gttccctgaa attattcccc tacttgacta ataagtatat aaagacggta     5640

ggtattgatt gtaattctgt aaatctattt cttaaacttc ttaaattcta cttttatagt     5700

tagtcttttt tttagtttta aaacaccaga acttagtttc gacggattct agaactagtg     5760

gatccaaaaa aatgtctgat gcggctcctt cattgagcaa tctattttat gatccaacgt     5820

ataatcctgg tcaaagcacc attaactaca cttccatata tgggaatgga tctaccatca     5880

ctttcgatga gttgcaaggt ttagttaaca gtactgttac tcaggccatt atgtttggtg     5940

tcagatgtgg tgcagctgct ttgactttga ttgtcatgtg gatgacatcg agaagcagaa     6000

aaacgccgat tttcattatc aaccaagttt cattgttttt aatcattttg cattctgcac     6060

tctattttaa atatttactg tctaattact cttcagtgac ttacgctctc accggatttc     6120

ctcagttcat cagtagaggt gacgttcatg tttatggtgc tacaaatata attcaagtcc     6180

ttcttgtggc ttctattgag acttcactgg tgtttcagat aaaagttatt ttcacaggcg     6240

acaacttcaa aaggataggt ttgatgctga cgtcgatatc tttcacttta gggattgcta     6300

cagttaccat gtattttgta agcgctgtta aaggtatgat tgtgacttat aatgatgtta     6360

gtgccaccca agataaatac ttcaatgcat ccacaatttt acttgcatcc tcaataaact     6420

ttatgtcatt tgtcctggta gttaaattga ttttagctat tagatcaaga agattccttg     6480

gtctcaagca gttcgatagt ttccatattt tactcataat gtcatgtcaa tctttgttgg     6540

ttccatcgat aatattcatc ctcgcataca gtttgaaacc aaaccaggga acagatgtct     6600

tgactactgt tgcaacatta cttgctgtat tgtctttacc attatcatca atgtgggcca     6660

cggctgctaa taatgcatcc aaaacaaaca caattacttc agactttaca acatccacag     6720

ataggtttta tccaggcacg ctgtctagct ttcaaactga tagtatcaac aacgatgcta     6780

aaagcagtct cagaagtaga ttatatgacc tatatcctag aaggaaggaa acaacatcgg     6840

ataaacattc ggaaagaact tttgtttctg agactgcaga tgatatagag aaaaatcagt     6900

tttatcagtt gcccacacct acgagttcaa aaaatactag gataggaccg tttgctgatg     6960

caagttacaa agagggagaa gttgaacccg tcgacatgta cactcccgat acggcagctg     7020

atgaggaagc cagaaagttc tggactgaag ataataataa tttatga                   7067


