                         SEQUENCE LISTING

<110>  LUPIN LIMITED
 
<120>  Dual cistronic bacterial expression system

<130>  FPAA4613PCT

<150>  2245/MUM/2014
<151>  2014-07-09

<160>  19    

<170>  PatentIn version 3.5

<210>  1
<211>  762
<212>  DNA
<213>  artificial

<220>
<223>  Ranibizumab heavy chain sequence

<400>  1
atgaaatacc tgctgccgac agctgctgct ggtctgctgc tcctcgctgc ccagccggcg       60

atggccgaag tccaactggt cgaatcgggt ggtggtctgg tccaaccggg tggctctctg      120

cgtctgtcgt gtgctgcctc gggctatgat tttacccatt atggtatgaa ctgggtccgt      180

caggccccgg gtaaaggtct ggaatgggtg ggctggatta atacctacac gggtgaaccg      240

acctatgcgg ccgattttaa acgtcgcttt acgttctctc tggacacctc gaagagcacg      300

gcatatctgc agatgaacag tctgcgcgcg gaagataccg ccgtgtatta ctgcgcgaag      360

tacccgtatt actatggcac gtcccactgg tattttgacg tttggggcca aggtaccctg      420

gtcaccgtga gcagcgcgag caccaaaggc ccgagcgtgt tcccgctggc cccgagttcc      480

aagtctacca gtggcggtac ggcagctctg ggttgtctgg ttaaagatta ttttccggaa      540

ccggttaccg tctcctggaa cagcggcgca ctgacctctg gtgtgcatac gttcccggct      600

gttctgcagt catcgggcct gtacagcctg agcagcgtgg ttaccgttcc gagttcctca      660

ctgggtaccc aaacgtatat ctgcaacgtc aatcacaaac cgagcaatac caaagtggac      720

aaaaaagtgg aaccgaaatc gtgtgataaa acgcatctgt aa                         762


<210>  2
<211>  711
<212>  DNA
<213>  artificial

<220>
<223>  Ranibizumab Light chain: 2295-3005Nt seq

<400>  2
atgaaatacc tgctgccgac agctgctgct ggtctgctgc tcctcgctgc ccagccggcg       60

atggccgaca ttcaactgac gcaaagtccg agcagcctga gcgcatccgt gggcgaccgt      120

gtgacgatta cctgttccgc aagccaagac atctctaact atctgaattg gtaccagcaa      180

aaaccgggca aggcaccgaa agtcctgatt tattttacca gctctctgca ttccggcgtt      240

ccgtcacgtt ttagcggctc tggtagtggc accgatttca ccctgacgat cagttccctg      300

cagccggaag actttgctac gtattactgc cagcaataca gcaccgtgcc gtggacgttc      360

ggtcagggca ccaaggttga aattaaacgt acggttgcgg ccccgtctgt ctttatcttc      420

ccgccgagtg atgaacagct gaaatcgggt accgcaagcg tggtttgtct gctgaacaat      480

ttctatccgc gcgaagcaaa ggtccagtgg aaagtggaca acgctctgca gtccggcaat      540

tcacaagaat cggtgaccga acaagatagc aaggactcta cgtacagtct gtcatcgacc      600

ctgacgctgt ccaaagcgga ttatgaaaaa cacaaggttt acgcctgcga agtcacccat      660

caaggtctgt cgtctccggt taccaagagt ttcaatcgtg gcgaatgtta a               711


<210>  3
<211>  253
<212>  PRT
<213>  artificial

<220>
<223>  Ranibizumab heavy chain amino acid sequence

<400>  3

Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala 
1               5                   10                  15      


Ala Gln Pro Ala Met Ala Glu Val Gln Leu Val Glu Ser Gly Gly Gly 
            20                  25                  30          


Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly 
        35                  40                  45              


Tyr Asp Phe Thr His Tyr Gly Met Asn Trp Val Arg Gln Ala Pro Gly 
    50                  55                  60                  


Lys Gly Leu Glu Trp Val Gly Trp Ile Asn Thr Tyr Thr Gly Glu Pro 
65                  70                  75                  80  


Thr Tyr Ala Ala Asp Phe Lys Arg Arg Phe Thr Phe Ser Leu Asp Thr 
                85                  90                  95      


Ser Lys Ser Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp 
            100                 105                 110         


Thr Ala Val Tyr Tyr Cys Ala Lys Tyr Pro Tyr Tyr Tyr Gly Thr Ser 
        115                 120                 125             


His Trp Tyr Phe Asp Val Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
    130                 135                 140                 


Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser 
145                 150                 155                 160 


Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp 
                165                 170                 175     


Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr 
            180                 185                 190         


Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr 
        195                 200                 205             


Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln 
    210                 215                 220                 


Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp 
225                 230                 235                 240 


Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Leu 
                245                 250             


<210>  4
<211>  236
<212>  PRT
<213>  artificial

<220>
<223>  Ranibizumab Light chain Amino acid sequence

<400>  4

Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala 
1               5                   10                  15      


Ala Gln Pro Ala Met Ala Asp Ile Gln Leu Thr Gln Ser Pro Ser Ser 
            20                  25                  30          


Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Ser Ala Ser 
        35                  40                  45              


Gln Asp Ile Ser Asn Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Gly Lys 
    50                  55                  60                  


Ala Pro Lys Val Leu Ile Tyr Phe Thr Ser Ser Leu His Ser Gly Val 
65                  70                  75                  80  


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
                85                  90                  95      


Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln 
            100                 105                 110         


Tyr Ser Thr Val Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile 
        115                 120                 125             


Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp 
    130                 135                 140                 


Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn 
145                 150                 155                 160 


Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu 
                165                 170                 175     


Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp 
            180                 185                 190         


Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr 
        195                 200                 205             


Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser 
    210                 215                 220                 


Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230                 235     


<210>  5
<211>  762
<212>  DNA
<213>  artificial

<220>
<223>  Heavy chain 940-178 complement

<400>  5
ttacagatgc gttttatcac acgatttcgg ttccactttt ttgtccactt tggtattgct       60

cggtttgtga ttgacgttgc agatatacgt ttgggtaccc agtgaggaac tcggaacggt      120

aaccacgctg ctcaggctgt acaggcccga tgactgcaga acagccggga acgtatgcac      180

accagaggtc agtgcgccgc tgttccagga gacggtaacc ggttccggaa aataatcttt      240

aaccagacaa cccagagctg ccgtaccgcc actggtagac ttggaactcg gggccagcgg      300

gaacacgctc gggcctttgg tgctcgcgct gctcacggtg accagggtac cttggcccca      360

aacgtcaaaa taccagtggg acgtgccata gtaatacggg tacttcgcgc agtaatacac      420

ggcggtatct tccgcgcgca gactgttcat ctgcagatat gccgtgctct tcgaggtgtc      480

cagagagaac gtaaagcgac gtttaaaatc ggccgcatag gtcggttcac ccgtgtaggt      540

attaatccag cccacccatt ccagaccttt acccggggcc tgacggaccc agttcatacc      600

ataatgggta aaatcatagc ccgaggcagc acacgacaga cgcagagagc cacccggttg      660

gaccagacca ccacccgatt cgaccagttg gacttcggcc atcgccggct gggcagcgag      720

gagcagcaga ccagcagcag ctgtcggcag caggtatttc at                         762


<210>  6
<211>  96
<212>  DNA
<213>  artificial

<220>
<223>  DNA sequence of Liraglutide

<400>  6
catgcagaag gcacctttac gagtgatgtg agctcttatc tggaaggcca ggcggccaaa       60

gaatttattg cgtggctggt tcgtggccgt ggttaa                                 96


<210>  7
<211>  31
<212>  PRT
<213>  artificial

<220>
<223>  Amino acid sequence of Liraglutide

<400>  7

His Ala Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
            20                  25                  30      


<210>  8
<211>  456
<212>  DNA
<213>  artificial

<220>
<223>  SAK nucleotide sequence (with EK site)

<400>  8
catatgtcaa gttcattcga caaaggaaaa tataaaaaag gcgatgacgc gagttatttt       60

gaaccaacag gcccgtattt gatggtaaat gtgactggag ttgatggtaa aggaaatgaa      120

ttgctatccc ctcattatgt cgagtttcct attaaacctg ggactacact tacaaaagaa      180

aaaattgaat acctgcagga tgatgatgat aaatacgtag aatgggcatt agatgcgaca      240

gcatataaag agtttagagt agttgaatta gatccaagcg caaagatcga agtcacttat      300

tatgataaga ataagaaaaa agaagaaacg aagtctttcc ctataacaga aaaaggtttt      360

gttgtcccag atttatcaga gcatattaaa aaccctggat tcaacttaat tacaaaggtt      420

gttatagaaa agaaaggatc cgatgatgat gataaa                                456


<210>  9
<211>  151
<212>  PRT
<213>  artificial

<220>
<223>  SAK amino acid sequence (with EK tag)

<400>  9

Met Ser Ser Ser Phe Asp Lys Gly Lys Tyr Lys Lys Gly Asp Asp Ala 
1               5                   10                  15      


Ser Tyr Phe Glu Pro Thr Gly Pro Tyr Leu Met Val Asn Val Thr Gly 
            20                  25                  30          


Val Asp Gly Lys Gly Asn Glu Leu Leu Ser Pro His Tyr Val Glu Phe 
        35                  40                  45              


Pro Ile Lys Pro Gly Thr Thr Leu Thr Lys Glu Lys Ile Glu Tyr Leu 
    50                  55                  60                  


Gln Asp Asp Asp Asp Lys Tyr Val Glu Trp Ala Leu Asp Ala Thr Ala 
65                  70                  75                  80  


Tyr Lys Glu Phe Arg Val Val Glu Leu Asp Pro Ser Ala Lys Ile Glu 
                85                  90                  95      


Val Thr Tyr Tyr Asp Lys Asn Lys Lys Lys Glu Glu Thr Lys Ser Phe 
            100                 105                 110         


Pro Ile Thr Glu Lys Gly Phe Val Val Pro Asp Leu Ser Glu His Ile 
        115                 120                 125             


Lys Asn Pro Gly Phe Asn Leu Ile Thr Lys Val Val Ile Glu Lys Lys 
    130                 135                 140                 


Gly Ser Asp Asp Asp Asp Lys 
145                 150     


<210>  10
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  T7 promoter: 1011-1027

<400>  10
ctatagtgag tcgtatt                                                      17


<210>  11
<211>  52
<212>  DNA
<213>  artificial

<220>
<223>  T7 terminator:26-72

<400>  11
ttcagcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc ta               52


<210>  12
<211>  10
<212>  DNA
<213>  artificial

<220>
<223>  RBS of pET21a: 947-957

<400>  12
tctccttctt                                                              10


<210>  13
<211>  879
<212>  DNA
<213>  artificial

<220>
<223>  AraC gene: 1048-1927

<400>  13
ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct       60

cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc      120

aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg      180

gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag      240

atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt      300

gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg      360

tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt      420

tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa      480

caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa      540

tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg      600

gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa      660

cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg      720

accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa      780

atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga      840

gtatcccggc agcaggggat cattttgcgc ttcagccat                             879


<210>  14
<211>  27
<212>  DNA
<213>  artificial

<220>
<223>  Arabinose promoter: 2203-2230

<400>  14
acgcttttta tcgcaactct ctactgt                                           27


<210>  15
<211>  425
<212>  DNA
<213>  artificial

<220>
<223>  Arabinose terminator: 3011-3438

<400>  15
gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa       60

gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca      120

tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga      180

gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt      240

cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg      300

gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact      360

gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa      420

actct                                                                  425


<210>  16
<211>  1084
<212>  DNA
<213>  artificial

<220>
<223>  LacI sequence: 3810-4890

<400>  16
tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt       60

ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc      120

ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca      180

gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt      240

cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga      300

acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag      360

tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg      420

cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat      480

tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca      540

gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg      600

ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg      660

gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac      720

tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc      780

cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc      840

atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag      900

cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc      960

cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg     1020

cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca     1080

gtga                                                                  1084


<210>  17
<211>  861
<212>  DNA
<213>  artificial

<220>
<223>  bla (ampicillin resistance): 7085-7942

<400>  17
ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat       60

agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc      120

cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa      180

ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca      240

gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa      300

cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt      360

cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc      420

ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact      480

catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc      540

tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg      600

ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct      660

catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc      720

cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag      780

cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac      840

acggaaatgt tgaatactca t                                                861


<210>  18
<211>  1
<212>  DNA
<213>  artificial

<220>
<223>  pBR322 origin 6323 sequence

<400>  18
t                                                                        1


<210>  19
<211>  8540
<212>  DNA
<213>  artificial

<220>
<223>  Complete clone sequence of dual cistron vector including heavy 
       and light chainfab

<400>  19
atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa       60

ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt      120

tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagctttt      180

acagatgcgt tttatcacac gatttcggtt ccactttttt gtccactttg gtattgctcg      240

gtttgtgatt gacgttgcag atatacgttt gggtacccag tgaggaactc ggaacggtaa      300

ccacgctgct caggctgtac aggcccgatg actgcagaac agccgggaac gtatgcacac      360

cagaggtcag tgcgccgctg ttccaggaga cggtaaccgg ttccggaaaa taatctttaa      420

ccagacaacc cagagctgcc gtaccgccac tggtagactt ggaactcggg gccagcggga      480

acacgctcgg gcctttggtg ctcgcgctgc tcacggtgac cagggtacct tggccccaaa      540

cgtcaaaata ccagtgggac gtgccatagt aatacgggta cttcgcgcag taatacacgg      600

cggtatcttc cgcgcgcaga ctgttcatct gcagatatgc cgtgctcttc gaggtgtcca      660

gagagaacgt aaagcgacgt ttaaaatcgg ccgcataggt cggttcaccc gtgtaggtat      720

taatccagcc cacccattcc agacctttac ccggggcctg acggacccag ttcataccat      780

aatgggtaaa atcatagccc gaggcagcac acgacagacg cagagagcca cccggttgga      840

ccagaccacc acccgattcg accagttgga cttcggccat cgccggctgg gcagcgagga      900

gcagcagacc agcagcagct gtcggcagca ggtatttcat atgtatatct ccttcttaaa      960

gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc ctatagtgag     1020

tcgtattaat ttcgcgggat cgagatcttt atgacaactt gacggctaca tcattcactt     1080

tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc     1140

gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc     1200

gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga     1260

cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat     1320

gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac     1380

aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc     1440

gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc     1500

cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat     1560

ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt     1620

aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac     1680

cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt     1740

ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt     1800

tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta     1860

aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt     1920

cagccatact tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca     1980

gacattgccg tcactgcgtc ttttactggc tcttctcgct aaccaaaccg gtaaccccgc     2040

ttattaaaag cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa     2100

gtgtctataa tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct     2160

atgccatagc atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac     2220

tctctactgt ttctccatac ccgttttttt gggctagaaa taattttgtt taactttaag     2280

aaggagatat acatatgaaa tacctgctgc cgacagctgc tgctggtctg ctgctcctcg     2340

ctgcccagcc ggcgatggcc gacattcaac tgacgcaaag tccgagcagc ctgagcgcat     2400

ccgtgggcga ccgtgtgacg attacctgtt ccgcaagcca agacatctct aactatctga     2460

attggtacca gcaaaaaccg ggcaaggcac cgaaagtcct gatttatttt accagctctc     2520

tgcattccgg cgttccgtca cgttttagcg gctctggtag tggcaccgat ttcaccctga     2580

cgatcagttc cctgcagccg gaagactttg ctacgtatta ctgccagcaa tacagcaccg     2640

tgccgtggac gttcggtcag ggcaccaagg ttgaaattaa acgtacggtt gcggccccgt     2700

ctgtctttat cttcccgccg agtgatgaac agctgaaatc gggtaccgca agcgtggttt     2760

gtctgctgaa caatttctat ccgcgcgaag caaaggtcca gtggaaagtg gacaacgctc     2820

tgcagtccgg caattcacaa gaatcggtga ccgaacaaga tagcaaggac tctacgtaca     2880

gtctgtcatc gaccctgacg ctgtccaaag cggattatga aaaacacaag gtttacgcct     2940

gcgaagtcac ccatcaaggt ctgtcgtctc cggttaccaa gagtttcaat cgtggcgaat     3000

gttaaaagct tggctgtttt ggcggatgag agaagatttt cagcctgata cagattaaat     3060

cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc     3120

cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt     3180

ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa     3240

gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat     3300

ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg ggcaggacgc     3360

ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt     3420

gcgtttctac aaactcttag atctcgatcc tctacgccgg acgcatcgtg gccggcatca     3480

ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc     3540

gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg     3600

tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc     3660

tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc     3720

gtcgagatcc cggacaccat cgaatggcgc aaaacctttc gcggtatggc atgatagcgc     3780

ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca     3840

gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt     3900

tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac     3960

cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt     4020

ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg     4080

ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg     4140

gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac     4200

caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc     4260

tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc     4320

gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt     4380

tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt     4440

cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg     4500

caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg     4560

ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta     4620

gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa     4680

caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc     4740

caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg     4800

gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca     4860

cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct     4920

cactcattag gcaccgggat ctcgaccgat gcccttgaga gccttcaacc cagtcagctc     4980

cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat     5040

gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg     5100

ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc     5160

tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc     5220

cggcatggcg gccccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg     5280

ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag     5340

cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg     5400

tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg     5460

catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg     5520

gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc     5580

ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct     5640

ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc     5700

agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt     5760

aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc     5820

gcttcacgac cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg     5880

tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc     5940

cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc     6000

catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag     6060

cagattgtac tgagagtgca ccatatatgc ggtgtgaaat accgcacaga tgcgtaagga     6120

gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg     6180

ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat     6240

caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta     6300

aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa     6360

atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc     6420

cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt     6480

ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca     6540

gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg     6600

accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat     6660

cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta     6720

cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct     6780

gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac     6840

aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa     6900

aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa     6960

actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt     7020

taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca     7080

gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca     7140

tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc     7200

ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa     7260

accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc     7320

agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca     7380

acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat     7440

tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag     7500

cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac     7560

tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt     7620

ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt     7680

gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc     7740

tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat     7800

ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca     7860

gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga     7920

cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg     7980

gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg     8040

ttccgcgcac atttccccga aaagtgccac ctgaaattgt aaacgttaat attttgttaa     8100

aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca     8160

aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga     8220

acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc     8280

agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc     8340

gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc     8400

cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg     8460

caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac     8520

agggcgcgtc ccattcgcca                                                 8540


