                         SEQUENCE LISTING

<110>  Agresti, Jeremy
       Cooper, Samantha
       Karlin-Neumann, George
       Heredia, Nick
       Lebofsky, Ronald
 
<120>  DIGITAL PCR BARCODING

<130>  94868-947922

<140>  WO PCT/US2015/037525
<141>  2015-06-25

<150>  US 62/016,568
<151>  2014-06-24

<160>  29    

<170>  PatentIn version 3.5

<210>  1
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(10)
<223>  N is A, C, G or T

<400>  1
nnnnnngtaa                                                              10


<210>  2
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(10)
<223>  N is A, C, G or T

<400>  2
nnnnnnacgc                                                              10


<210>  3
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(10)
<223>  N is A, C, G or T

<400>  3
nnnnnncatg                                                              10


<210>  4
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(10)
<223>  N is A, C, G or T

<400>  4
nnnnnntgct                                                              10


<210>  5
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(14)
<223>  N is A, C, G or T

<400>  5
nnnnnntgct nnnn                                                         14


<210>  6
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(14)
<223>  N is A, C, G or T

<400>  6
nnnnnncatg nnnn                                                         14


<210>  7
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(14)
<223>  N is A, C, G or T

<400>  7
nnnnnnacgc nnnn                                                         14


<210>  8
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(14)
<223>  N is A, C, G or T

<400>  8
nnnnnngtaa nnnn                                                         14


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  N is A, C, G or T

<400>  9
nnnnnntgct nnnnnnnnnn                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  N is A, C, G or T

<400>  10
nnnnnncatg nnnnnnnnnn                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  N is A, C, G or T

<400>  11
nnnnnnacgc nnnnnnnnnn                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(20)
<223>  N is A, C, G or T

<400>  12
nnnnnngtaa nnnnnnnnnn                                                   20


<210>  13
<211>  56
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  B is C, G, or T

<220>
<221>  misc_feature
<222>  (2)..(56)
<223>  N is A, C, G, or T

<400>  13
baaaaaaaaa aaaaaaaaaa aaaaannnnn nnnnnngcgt cctgacagga ggcttg           56


<210>  14
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(53)
<223>  N is A, C, G or T

<400>  14
gcgtcctgac aggaggcttg nnnnnnnnnn agatcggaag agcacacgtc tga              53


<210>  15
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide

<400>  15
tcagacgtgt gctcttccga tct                                               23


<210>  16
<211>  88
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  B is C, G, or T

<220>
<221>  misc_feature
<222>  (2)..(88)
<223>  N is A, C, G or T

<400>  16
baaaaaaaaa aaaaaaaaaa aaaaannnnn nnnnngcgtc ctgacaggag gcttgnnnnn       60

nnnnnagatc ggaagagcac acgtctga                                          88


<210>  17
<211>  88
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(87)
<223>  N is A, C, G, or T

<220>
<221>  misc_feature
<222>  (88)..(88)
<223>  V is A, C, or G

<400>  17
tcagacgtgt gctcttccga tctnnnnnnn nnncaagcct cctgtcagga cgcnnnnnnn       60

nnnttttttt tttttttttt tttttttv                                          88


<210>  18
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  B is C, G, or U (or T)

<400>  18
baaaaaaaaa aaaaaaaaaa aaaaa                                             25


<210>  19
<211>  79
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (79)..(79)
<223>  V is A, C, or G

<400>  19
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatcttt       60

tttttttttt ttttttttv                                                    79


<210>  20
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide - represents 5' end flanking an index 
       barcode oligonucleotide sequence

<400>  20
caagcagaag acggcatacg agat                                              24


<210>  21
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide - represents 3' end flanking an index 
       barcode oligonucleotide sequence

<400>  21
gtgactggag ttcagacgtg tgctcttccg atct                                   34


<210>  22
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide

<400>  22
tctagccttc tcgcagcaca tccctttctc acatctagag ccaccagcgg catagtaa         58


<210>  23
<211>  202
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(202)
<223>  N is A, C, G, or T

<220>
<221>  misc_feature
<222>  (79)..(79)
<223>  V is A, C, or G

<220>
<221>  misc_feature
<222>  (79)..(79)
<223>  3' end is flanked by cDNA sequence (sequence/length variable and 
       unknown)

<220>
<221>  misc_feature
<222>  (80)..(80)
<223>  B is C, G, or T

<220>
<221>  misc_feature
<222>  (80)..(80)
<223>  5' end is preceded by cDNA sequence (sequence/length variable and
       unknown)

<220>
<221>  misc_feature
<222>  (178)..(178)
<223>  3' end is flanked by index barcode sequence (sequence/length 
       variable and unknown)

<220>
<221>  misc_feature
<222>  (179)..(179)
<223>  5' end is preceded by index barcode sequence (sequence/length 
       variable and unknown)

<400>  23
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatcttt       60

tttttttttt ttttttttvb aaaaaaaaaa aaaaaaaaaa aaaannnnnn nnnngcgtcc      120

tgacaggagg cttgnnnnnn nnnnagatcg gaagagcaca cgtctgaact ccagtcacat      180

ctcgtatgcc gtcttctgct tg                                               202


<210>  24
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide primer

<400>  24
gtgactggag ttcagacgtg tgctcttccg atct                                   34


<210>  25
<211>  202
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(202)
<223>  N is A, C, G, or T

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  3' end is flanked by index barcode sequence (sequence/length 
       variable and unknown)

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  5' end is preceded by index barcode sequence (sequence/length 
       variable and unknown)

<220>
<221>  misc_feature
<222>  (123)..(123)
<223>  3' end is flanked by cDNA sequence (sequence/length variable and 
       unknown)

<220>
<221>  misc_feature
<222>  (123)..(123)
<223>  V is A, C, and G

<220>
<221>  misc_feature
<222>  (124)..(124)
<223>  B is C, G, or T

<220>
<221>  misc_feature
<222>  (124)..(124)
<223>  5' end is preceded by cDNA sequence (sequence/length variable and
       unknown)

<400>  25
caagcagaag acggcatacg agatgtgact ggagttcaga cgtgtgctct tccgatctnn       60

nnnnnnnnca agcctcctgt caggacgcnn nnnnnnnntt tttttttttt tttttttttt      120

ttvbaaaaaa aaaaaaaaaa aaaaagatcg gaagagcgtc gtgtagggaa agagtgtaga      180

tctcggtggt cgccgtatca tt                                               202


<210>  26
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide primer

<400>  26
acactctttc cctacacgac gctcttccga tct                                    33


<210>  27
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide primer

<400>  27
agatcggaag agcacacgtc tgaactccag tc                                     32


<210>  28
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' Hexachloro-fluorescein (HEX) labeled

<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  3' black hole quencher (BH3) labeled

<400>  28
aagagcgtcg tgtagggaaa gagtgtaga                                         29


<210>  29
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' fluorescein (FAM) labeled

<220>
<221>  misc_feature
<222>  (26)..(26)
<223>  3' black hole quencher (BHQ3) labeled

<400>  29
agagcacacg tctgaactcc agtcac                                            26


