                         SEQUENCE LISTING

<110>  PharmaZell GmbH
 
<120>  Neuartige Ganzzellreduktion von Dehydrocholsure und Neuartige 
       7-Hydroxysteroiddehydrogenase-Mutanten und deren Verwendung

<130>  M/54141

<160>  21    

<170>  PatentIn version 3.5

<210>  1
<211>  792
<212>  DNA
<213>  Collinsella aerofaciens


<220>
<221>  CDS
<222>  (1)..(792)

<400>  1
atg aac ctg agg gag aag tac ggt gag tgg ggc ctg atc ctg ggc gcg         48
Met Asn Leu Arg Glu Lys Tyr Gly Glu Trp Gly Leu Ile Leu Gly Ala           
1               5                   10                  15                

acc gag ggc gtc ggc aag gcg ttc tgc gag aag atc gcc gcc ggc ggc         96
Thr Glu Gly Val Gly Lys Ala Phe Cys Glu Lys Ile Ala Ala Gly Gly           
            20                  25                  30                    

atg aac gtc gtc atg gtc ggc cgt cgc gag gag aag ctg aac gtg ctc        144
Met Asn Val Val Met Val Gly Arg Arg Glu Glu Lys Leu Asn Val Leu           
        35                  40                  45                        

gca ggc gag atc cgc gag acc tac ggc gtg gag acc aag gtc gtg cgc        192
Ala Gly Glu Ile Arg Glu Thr Tyr Gly Val Glu Thr Lys Val Val Arg           
    50                  55                  60                            

gcc gac ttt agc cag ccc ggc gct gcc gag acc gtc ttc gcc gcg acc        240
Ala Asp Phe Ser Gln Pro Gly Ala Ala Glu Thr Val Phe Ala Ala Thr           
65                  70                  75                  80            

gag ggc ctg gac atg ggc ttc atg agc tac gtg gcc tgc ctg cac agc        288
Glu Gly Leu Asp Met Gly Phe Met Ser Tyr Val Ala Cys Leu His Ser           
                85                  90                  95                

ttc ggt aag atc cag gac acc ccc tgg gag aag cac gag gcc atg atc        336
Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu Lys His Glu Ala Met Ile           
            100                 105                 110                   

aac gtc aac gtc gtg acc ttc ctc aag tgc ttc cac cac tac atg cgg        384
Asn Val Asn Val Val Thr Phe Leu Lys Cys Phe His His Tyr Met Arg           
        115                 120                 125                       

atc ttt gcc gcc cag gac cgc ggc gcc gtg atc aac gtc tcg tcg atg        432
Ile Phe Ala Ala Gln Asp Arg Gly Ala Val Ile Asn Val Ser Ser Met           
    130                 135                 140                           

acc ggc atc agc tcc agc ccc tgg aac ggc cag tac ggc gcg ggc aag        480
Thr Gly Ile Ser Ser Ser Pro Trp Asn Gly Gln Tyr Gly Ala Gly Lys           
145                 150                 155                 160           

gcc ttc atc ctc aag atg acc gag gcc gtg gcc tgc gag tgc gag ggc        528
Ala Phe Ile Leu Lys Met Thr Glu Ala Val Ala Cys Glu Cys Glu Gly           
                165                 170                 175               

acc ggc gtc gac gtc gag gtc atc acc ctc ggc acc acc cta acc ccc        576
Thr Gly Val Asp Val Glu Val Ile Thr Leu Gly Thr Thr Leu Thr Pro           
            180                 185                 190                   

agc ctg ctg tcc aac ctc ccc ggc ggc ccg cag ggc gag gcc gtc atg        624
Ser Leu Leu Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu Ala Val Met           
        195                 200                 205                       

aag atc gcc ctc acc ccc gag gag tgc gtt gac gag gcc ttt gag aag        672
Lys Ile Ala Leu Thr Pro Glu Glu Cys Val Asp Glu Ala Phe Glu Lys           
    210                 215                 220                           

ctg ggt aag gag ctc tcc gtc atc gcc ggc cag cgc aac aag gac tcc        720
Leu Gly Lys Glu Leu Ser Val Ile Ala Gly Gln Arg Asn Lys Asp Ser           
225                 230                 235                 240           

gtc cac gac tgg aag gca aac cac acc gag gac gag tac atc cgc tac        768
Val His Asp Trp Lys Ala Asn His Thr Glu Asp Glu Tyr Ile Arg Tyr           
                245                 250                 255               

atg ggg tcg ttc tac cgc gac tag                                        792
Met Gly Ser Phe Tyr Arg Asp                                               
            260                                                           


<210>  2
<211>  263
<212>  PRT
<213>  Collinsella aerofaciens

<400>  2

Met Asn Leu Arg Glu Lys Tyr Gly Glu Trp Gly Leu Ile Leu Gly Ala 
1               5                   10                  15      


Thr Glu Gly Val Gly Lys Ala Phe Cys Glu Lys Ile Ala Ala Gly Gly 
            20                  25                  30          


Met Asn Val Val Met Val Gly Arg Arg Glu Glu Lys Leu Asn Val Leu 
        35                  40                  45              


Ala Gly Glu Ile Arg Glu Thr Tyr Gly Val Glu Thr Lys Val Val Arg 
    50                  55                  60                  


Ala Asp Phe Ser Gln Pro Gly Ala Ala Glu Thr Val Phe Ala Ala Thr 
65                  70                  75                  80  


Glu Gly Leu Asp Met Gly Phe Met Ser Tyr Val Ala Cys Leu His Ser 
                85                  90                  95      


Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu Lys His Glu Ala Met Ile 
            100                 105                 110         


Asn Val Asn Val Val Thr Phe Leu Lys Cys Phe His His Tyr Met Arg 
        115                 120                 125             


Ile Phe Ala Ala Gln Asp Arg Gly Ala Val Ile Asn Val Ser Ser Met 
    130                 135                 140                 


Thr Gly Ile Ser Ser Ser Pro Trp Asn Gly Gln Tyr Gly Ala Gly Lys 
145                 150                 155                 160 


Ala Phe Ile Leu Lys Met Thr Glu Ala Val Ala Cys Glu Cys Glu Gly 
                165                 170                 175     


Thr Gly Val Asp Val Glu Val Ile Thr Leu Gly Thr Thr Leu Thr Pro 
            180                 185                 190         


Ser Leu Leu Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu Ala Val Met 
        195                 200                 205             


Lys Ile Ala Leu Thr Pro Glu Glu Cys Val Asp Glu Ala Phe Glu Lys 
    210                 215                 220                 


Leu Gly Lys Glu Leu Ser Val Ile Ala Gly Gln Arg Asn Lys Asp Ser 
225                 230                 235                 240 


Val His Asp Trp Lys Ala Asn His Thr Glu Asp Glu Tyr Ile Arg Tyr 
                245                 250                 255     


Met Gly Ser Phe Tyr Arg Asp 
            260             


<210>  3
<211>  774
<212>  DNA
<213>  Comamonas testosteroni


<220>
<221>  CDS
<222>  (1)..(774)

<400>  3
atg tcc atc atc gtg ata agc ggc tgc gcc acc ggc att ggt gcc gct         48
Met Ser Ile Ile Val Ile Ser Gly Cys Ala Thr Gly Ile Gly Ala Ala           
1               5                   10                  15                

acg cgc aag gtc ctg gag gcg gcc ggt cac cag atc gta ggc atc gat         96
Thr Arg Lys Val Leu Glu Ala Ala Gly His Gln Ile Val Gly Ile Asp           
            20                  25                  30                    

ata cgc gat gcg gaa gtg att gcc gat ctc tcg acg gcc gaa ggt cga        144
Ile Arg Asp Ala Glu Val Ile Ala Asp Leu Ser Thr Ala Glu Gly Arg           
        35                  40                  45                        

aag cag gcg att gcc gat gta ctg gcg aag tgc agc aag ggc atg gac        192
Lys Gln Ala Ile Ala Asp Val Leu Ala Lys Cys Ser Lys Gly Met Asp           
    50                  55                  60                            

ggc ctg gtg ctg tgc gcc ggc ctg gga ccg cag acc aag gtg ctt ggc        240
Gly Leu Val Leu Cys Ala Gly Leu Gly Pro Gln Thr Lys Val Leu Gly           
65                  70                  75                  80            

aat gtg gtt tcg gtc aat tat ttt ggc gcg acc gag ctg atg gat gcc        288
Asn Val Val Ser Val Asn Tyr Phe Gly Ala Thr Glu Leu Met Asp Ala           
                85                  90                  95                

ttt ttg cca gcg ctg aaa aaa ggc cat cag ccc gca gcc gtc gtc atc        336
Phe Leu Pro Ala Leu Lys Lys Gly His Gln Pro Ala Ala Val Val Ile           
            100                 105                 110                   

tcg tcc gtg gct tcc gcg cat ctg gct ttt gac aag aac cca ctg gcg        384
Ser Ser Val Ala Ser Ala His Leu Ala Phe Asp Lys Asn Pro Leu Ala           
        115                 120                 125                       

ctg gca ctg gaa gcc ggc gag gaa gcc aag gcc cgc gcc att gtc gaa        432
Leu Ala Leu Glu Ala Gly Glu Glu Ala Lys Ala Arg Ala Ile Val Glu           
    130                 135                 140                           

cat gcg gga gag cag ggc gga aat ctg gcc tat gcg ggc agc aag aat        480
His Ala Gly Glu Gln Gly Gly Asn Leu Ala Tyr Ala Gly Ser Lys Asn           
145                 150                 155                 160           

gct ttg acg gtg gct gtg cgc aaa cgc gcc gcc gcc tgg ggc gag gct        528
Ala Leu Thr Val Ala Val Arg Lys Arg Ala Ala Ala Trp Gly Glu Ala           
                165                 170                 175               

ggc gtg cgc ctg aac acc atc gcc ccc ggt gca acc gag act ccc ttg        576
Gly Val Arg Leu Asn Thr Ile Ala Pro Gly Ala Thr Glu Thr Pro Leu           
            180                 185                 190                   

ctg cag gcg ggc ctg cag gac ccg cgc tat ggc gaa tcc att gcc aag        624
Leu Gln Ala Gly Leu Gln Asp Pro Arg Tyr Gly Glu Ser Ile Ala Lys           
        195                 200                 205                       

ttc gtt cct ccc atg ggc cgc cgt gcc gag ccg tcc gag atg gcg tcg        672
Phe Val Pro Pro Met Gly Arg Arg Ala Glu Pro Ser Glu Met Ala Ser           
    210                 215                 220                           

gtc atc gcc ttt ttg atg agc ccg gcc gca agc tat gtg cat ggc gcg        720
Val Ile Ala Phe Leu Met Ser Pro Ala Ala Ser Tyr Val His Gly Ala           
225                 230                 235                 240           

cag atc gtc att gat ggc ggc att gat gcg gtg atg cgc ccg aca cag        768
Gln Ile Val Ile Asp Gly Gly Ile Asp Ala Val Met Arg Pro Thr Gln           
                245                 250                 255               

ttc tga                                                                774
Phe                                                                       
                                                                          


<210>  4
<211>  257
<212>  PRT
<213>  Comamonas testosteroni

<400>  4

Met Ser Ile Ile Val Ile Ser Gly Cys Ala Thr Gly Ile Gly Ala Ala 
1               5                   10                  15      


Thr Arg Lys Val Leu Glu Ala Ala Gly His Gln Ile Val Gly Ile Asp 
            20                  25                  30          


Ile Arg Asp Ala Glu Val Ile Ala Asp Leu Ser Thr Ala Glu Gly Arg 
        35                  40                  45              


Lys Gln Ala Ile Ala Asp Val Leu Ala Lys Cys Ser Lys Gly Met Asp 
    50                  55                  60                  


Gly Leu Val Leu Cys Ala Gly Leu Gly Pro Gln Thr Lys Val Leu Gly 
65                  70                  75                  80  


Asn Val Val Ser Val Asn Tyr Phe Gly Ala Thr Glu Leu Met Asp Ala 
                85                  90                  95      


Phe Leu Pro Ala Leu Lys Lys Gly His Gln Pro Ala Ala Val Val Ile 
            100                 105                 110         


Ser Ser Val Ala Ser Ala His Leu Ala Phe Asp Lys Asn Pro Leu Ala 
        115                 120                 125             


Leu Ala Leu Glu Ala Gly Glu Glu Ala Lys Ala Arg Ala Ile Val Glu 
    130                 135                 140                 


His Ala Gly Glu Gln Gly Gly Asn Leu Ala Tyr Ala Gly Ser Lys Asn 
145                 150                 155                 160 


Ala Leu Thr Val Ala Val Arg Lys Arg Ala Ala Ala Trp Gly Glu Ala 
                165                 170                 175     


Gly Val Arg Leu Asn Thr Ile Ala Pro Gly Ala Thr Glu Thr Pro Leu 
            180                 185                 190         


Leu Gln Ala Gly Leu Gln Asp Pro Arg Tyr Gly Glu Ser Ile Ala Lys 
        195                 200                 205             


Phe Val Pro Pro Met Gly Arg Arg Ala Glu Pro Ser Glu Met Ala Ser 
    210                 215                 220                 


Val Ile Ala Phe Leu Met Ser Pro Ala Ala Ser Tyr Val His Gly Ala 
225                 230                 235                 240 


Gln Ile Val Ile Asp Gly Gly Ile Asp Ala Val Met Arg Pro Thr Gln 
                245                 250                 255     


Phe 
    


<210>  5
<211>  768
<212>  DNA
<213>  Escherichia coli


<220>
<221>  CDS
<222>  (1)..(768)

<400>  5
gtg ttt aat tct gac aac ctg aga ctc gac gga aaa tgc gcc atc atc         48
Val Phe Asn Ser Asp Asn Leu Arg Leu Asp Gly Lys Cys Ala Ile Ile           
1               5                   10                  15                

aca ggt gcg ggt gca ggt att ggt aaa gaa atc gcc att aca ttc gcg         96
Thr Gly Ala Gly Ala Gly Ile Gly Lys Glu Ile Ala Ile Thr Phe Ala           
            20                  25                  30                    

aca gct ggc gca tct gtg gtg gtc agt gat att aac gcc gac gca gct        144
Thr Ala Gly Ala Ser Val Val Val Ser Asp Ile Asn Ala Asp Ala Ala           
        35                  40                  45                        

aac cat gtt gta gac gaa att caa caa ctg ggt ggt cag gca ttt gcc        192
Asn His Val Val Asp Glu Ile Gln Gln Leu Gly Gly Gln Ala Phe Ala           
    50                  55                  60                            

tgc cgt tgt gat att act tcc gaa cag gaa ctc tct gca ctg gca gac        240
Cys Arg Cys Asp Ile Thr Ser Glu Gln Glu Leu Ser Ala Leu Ala Asp           
65                  70                  75                  80            

ttt gct atc agt aag ctg ggt aaa gtt gat att ctg gtt aac aac gcc        288
Phe Ala Ile Ser Lys Leu Gly Lys Val Asp Ile Leu Val Asn Asn Ala           
                85                  90                  95                

ggt ggc ggt gga cct aaa ccg ttt gat atg cca atg gcg gat ttt cgc        336
Gly Gly Gly Gly Pro Lys Pro Phe Asp Met Pro Met Ala Asp Phe Arg           
            100                 105                 110                   

cgt gct tat gaa ctg aat gtg ttt tct ttt ttc cat ctg tca caa ctt        384
Arg Ala Tyr Glu Leu Asn Val Phe Ser Phe Phe His Leu Ser Gln Leu           
        115                 120                 125                       

gtt gcg cca gaa atg gaa aaa aat ggc ggt ggc gtt att ctg acc atc        432
Val Ala Pro Glu Met Glu Lys Asn Gly Gly Gly Val Ile Leu Thr Ile           
    130                 135                 140                           

act tct atg gcg gca gaa aat aaa aat ata aac atg act tcc tat gca        480
Thr Ser Met Ala Ala Glu Asn Lys Asn Ile Asn Met Thr Ser Tyr Ala           
145                 150                 155                 160           

tca tct aaa gct gcg gcc agt cat ctg gtc aga aat atg gcg ttt gac        528
Ser Ser Lys Ala Ala Ala Ser His Leu Val Arg Asn Met Ala Phe Asp           
                165                 170                 175               

cta ggt gaa aaa aat att cgg gta aat ggc att gcg ccg ggg gca ata        576
Leu Gly Glu Lys Asn Ile Arg Val Asn Gly Ile Ala Pro Gly Ala Ile           
            180                 185                 190                   

tta acc gat gcc ctg aaa tcc gtt att aca cca gaa att gaa caa aaa        624
Leu Thr Asp Ala Leu Lys Ser Val Ile Thr Pro Glu Ile Glu Gln Lys           
        195                 200                 205                       

atg tta cag cac acg ccg atc aga cgt ctg ggc caa ccg caa gat att        672
Met Leu Gln His Thr Pro Ile Arg Arg Leu Gly Gln Pro Gln Asp Ile           
    210                 215                 220                           

gct aac gca gcg ctg ttc ctt tgc tcg cct gct gcg agc tgg gta agc        720
Ala Asn Ala Ala Leu Phe Leu Cys Ser Pro Ala Ala Ser Trp Val Ser           
225                 230                 235                 240           

gga caa att ctc acc gtc tcc ggt ggt ggg gta cag gag ctc aat taa        768
Gly Gln Ile Leu Thr Val Ser Gly Gly Gly Val Gln Glu Leu Asn               
                245                 250                 255               


<210>  6
<211>  255
<212>  PRT
<213>  Escherichia coli

<400>  6

Val Phe Asn Ser Asp Asn Leu Arg Leu Asp Gly Lys Cys Ala Ile Ile 
1               5                   10                  15      


Thr Gly Ala Gly Ala Gly Ile Gly Lys Glu Ile Ala Ile Thr Phe Ala 
            20                  25                  30          


Thr Ala Gly Ala Ser Val Val Val Ser Asp Ile Asn Ala Asp Ala Ala 
        35                  40                  45              


Asn His Val Val Asp Glu Ile Gln Gln Leu Gly Gly Gln Ala Phe Ala 
    50                  55                  60                  


Cys Arg Cys Asp Ile Thr Ser Glu Gln Glu Leu Ser Ala Leu Ala Asp 
65                  70                  75                  80  


Phe Ala Ile Ser Lys Leu Gly Lys Val Asp Ile Leu Val Asn Asn Ala 
                85                  90                  95      


Gly Gly Gly Gly Pro Lys Pro Phe Asp Met Pro Met Ala Asp Phe Arg 
            100                 105                 110         


Arg Ala Tyr Glu Leu Asn Val Phe Ser Phe Phe His Leu Ser Gln Leu 
        115                 120                 125             


Val Ala Pro Glu Met Glu Lys Asn Gly Gly Gly Val Ile Leu Thr Ile 
    130                 135                 140                 


Thr Ser Met Ala Ala Glu Asn Lys Asn Ile Asn Met Thr Ser Tyr Ala 
145                 150                 155                 160 


Ser Ser Lys Ala Ala Ala Ser His Leu Val Arg Asn Met Ala Phe Asp 
                165                 170                 175     


Leu Gly Glu Lys Asn Ile Arg Val Asn Gly Ile Ala Pro Gly Ala Ile 
            180                 185                 190         


Leu Thr Asp Ala Leu Lys Ser Val Ile Thr Pro Glu Ile Glu Gln Lys 
        195                 200                 205             


Met Leu Gln His Thr Pro Ile Arg Arg Leu Gly Gln Pro Gln Asp Ile 
    210                 215                 220                 


Ala Asn Ala Ala Leu Phe Leu Cys Ser Pro Ala Ala Ser Trp Val Ser 
225                 230                 235                 240 


Gly Gln Ile Leu Thr Val Ser Gly Gly Gly Val Gln Glu Leu Asn 
                245                 250                 255 


<210>  7
<211>  786
<212>  DNA
<213>  Bacillus subtilis

<400>  7
atgtatccgg atttaaaagg aaaagtcgtc gctattacag gagctgcttc agggctcgga       60

aaggcgatgg ccattcgctt cggcaaggag caggcaaaag tggttatcaa ctattatagt      120

aataaacaag atccgaacga ggtaaaagaa gaggtcatca aggcgggcgg tgaagctgtt      180

gtcgtccaag gagatgtcac gaaagaggaa gatgtaaaaa atatcgtgca aacggcaatt      240

aaggagttcg gcacactcga tattatgatt aataatgccg gtcttgaaaa tcctgtgcca      300

tctcacgaaa tgccgctcaa ggattgggat aaagtcatcg gcacgaactt aacgggtgcc      360

tttttaggaa gccgtgaagc gattaaatat ttcgtagaaa acgatatcaa gggaaatgtc      420

attaacatgt ccagtgtgca cgaagtgatt ccttggccgt tatttgtcca ctatgcggca      480

agtaaaggcg ggataaagct gatgacagaa acattagcgt tggaatacgc gccgaagggc      540

attcgcgtca ataatattgg gccaggtgcg atcaacacgc caatcaatgc tgaaaaattc      600

gctgacccta aacagaaagc tgatgtagaa agcatgattc caatgggata tatcggcgaa      660

ccggaggaga tcgccgcagt agcagcctgg cttgcttcga aggaagccag ctacgtcaca      720

ggcatcacgt tattcgcgga cggcggtatg acacaatatc cttcattcca ggcaggccgc      780

ggttaa                                                                 786


<210>  8
<211>  261
<212>  PRT
<213>  Bacillus subtilis

<400>  8

Met Tyr Pro Asp Leu Lys Gly Lys Val Val Ala Ile Thr Gly Ala Ala 
1               5                   10                  15      


Ser Gly Leu Gly Lys Ala Met Ala Ile Arg Phe Gly Lys Glu Gln Ala 
            20                  25                  30          


Lys Val Val Ile Asn Tyr Tyr Ser Asn Lys Gln Asp Pro Asn Glu Val 
        35                  40                  45              


Lys Glu Glu Val Ile Lys Ala Gly Gly Glu Ala Val Val Val Gln Gly 
    50                  55                  60                  


Asp Val Thr Lys Glu Glu Asp Val Lys Asn Ile Val Gln Thr Ala Ile 
65                  70                  75                  80  


Lys Glu Phe Gly Thr Leu Asp Ile Met Ile Asn Asn Ala Gly Leu Glu 
                85                  90                  95      


Asn Pro Val Pro Ser His Glu Met Pro Leu Lys Asp Trp Asp Lys Val 
            100                 105                 110         


Ile Gly Thr Asn Leu Thr Gly Ala Phe Leu Gly Ser Arg Glu Ala Ile 
        115                 120                 125             


Lys Tyr Phe Val Glu Asn Asp Ile Lys Gly Asn Val Ile Asn Met Ser 
    130                 135                 140                 


Ser Val His Glu Val Ile Pro Trp Pro Leu Phe Val His Tyr Ala Ala 
145                 150                 155                 160 


Ser Lys Gly Gly Ile Lys Leu Met Thr Glu Thr Leu Ala Leu Glu Tyr 
                165                 170                 175     


Ala Pro Lys Gly Ile Arg Val Asn Asn Ile Gly Pro Gly Ala Ile Asn 
            180                 185                 190         


Thr Pro Ile Asn Ala Glu Lys Phe Ala Asp Pro Lys Gln Lys Ala Asp 
        195                 200                 205             


Val Glu Ser Met Ile Pro Met Gly Tyr Ile Gly Glu Pro Glu Glu Ile 
    210                 215                 220                 


Ala Ala Val Ala Ala Trp Leu Ala Ser Lys Glu Ala Ser Tyr Val Thr 
225                 230                 235                 240 


Gly Ile Thr Leu Phe Ala Asp Gly Gly Met Thr Gln Tyr Pro Ser Phe 
                245                 250                 255     


Gln Ala Gly Arg Gly 
            260     


<210>  9
<211>  263
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  abgeleitet aus Collinsella aerofaciens (G39D)

<400>  9

Met Asn Leu Arg Glu Lys Tyr Gly Glu Trp Gly Leu Ile Leu Gly Ala 
1               5                   10                  15      


Thr Glu Gly Val Gly Lys Ala Phe Cys Glu Lys Ile Ala Ala Gly Gly 
            20                  25                  30          


Met Asn Val Val Met Val Asp Arg Arg Glu Glu Lys Leu Asn Val Leu 
        35                  40                  45              


Ala Gly Glu Ile Arg Glu Thr Tyr Gly Val Glu Thr Lys Val Val Arg 
    50                  55                  60                  


Ala Asp Phe Ser Gln Pro Gly Ala Ala Glu Thr Val Phe Ala Ala Thr 
65                  70                  75                  80  


Glu Gly Leu Asp Met Gly Phe Met Ser Tyr Val Ala Cys Leu His Ser 
                85                  90                  95      


Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu Lys His Glu Ala Met Ile 
            100                 105                 110         


Asn Val Asn Val Val Thr Phe Leu Lys Cys Phe His His Tyr Met Arg 
        115                 120                 125             


Ile Phe Ala Ala Gln Asp Arg Gly Ala Val Ile Asn Val Ser Ser Met 
    130                 135                 140                 


Thr Gly Ile Ser Ser Ser Pro Trp Asn Gly Gln Tyr Gly Ala Gly Lys 
145                 150                 155                 160 


Ala Phe Ile Leu Lys Met Thr Glu Ala Val Ala Cys Glu Cys Glu Gly 
                165                 170                 175     


Thr Gly Val Asp Val Glu Val Ile Thr Leu Gly Thr Thr Leu Thr Pro 
            180                 185                 190         


Ser Leu Leu Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu Ala Val Met 
        195                 200                 205             


Lys Ile Ala Leu Thr Pro Glu Glu Cys Val Asp Glu Ala Phe Glu Lys 
    210                 215                 220                 


Leu Gly Lys Glu Leu Ser Val Ile Ala Gly Gln Arg Asn Lys Asp Ser 
225                 230                 235                 240 


Val His Asp Trp Lys Ala Asn His Thr Glu Asp Glu Tyr Ile Arg Tyr 
                245                 250                 255     


Met Gly Ser Phe Tyr Arg Asp 
            260             


<210>  10
<211>  263
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  7-HSDH Mutante

<400>  10

Met Asn Leu Arg Glu Lys Tyr Gly Glu Trp Gly Leu Ile Leu Gly Ala 
1               5                   10                  15      


Thr Glu Gly Val Gly Lys Ala Phe Cys Glu Lys Ile Ala Ala Gly Gly 
            20                  25                  30          


Met Asn Val Val Met Val Asp Ile Arg Glu Glu Lys Leu Asn Val Leu 
        35                  40                  45              


Ala Gly Glu Ile Arg Glu Thr Tyr Gly Val Glu Thr Lys Val Val Arg 
    50                  55                  60                  


Ala Asp Phe Ser Gln Pro Gly Ala Ala Glu Thr Val Phe Ala Ala Thr 
65                  70                  75                  80  


Glu Gly Leu Asp Met Gly Phe Met Ser Tyr Val Ala Cys Leu His Ser 
                85                  90                  95      


Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu Lys His Glu Ala Met Ile 
            100                 105                 110         


Asn Val Asn Val Val Thr Phe Leu Lys Cys Phe His His Tyr Met Arg 
        115                 120                 125             


Ile Phe Ala Ala Gln Asp Arg Gly Ala Val Ile Asn Val Ser Ser Met 
    130                 135                 140                 


Thr Gly Ile Ser Ser Ser Pro Trp Asn Gly Gln Tyr Gly Ala Gly Lys 
145                 150                 155                 160 


Ala Phe Ile Leu Lys Met Thr Glu Ala Val Ala Cys Glu Cys Glu Gly 
                165                 170                 175     


Thr Gly Val Asp Val Glu Val Ile Thr Leu Gly Thr Thr Leu Thr Pro 
            180                 185                 190         


Ser Leu Leu Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu Ala Val Met 
        195                 200                 205             


Lys Ile Ala Leu Thr Pro Glu Glu Cys Val Asp Glu Ala Phe Glu Lys 
    210                 215                 220                 


Leu Gly Lys Glu Leu Ser Val Ile Ala Gly Gln Arg Asn Lys Asp Ser 
225                 230                 235                 240 


Val His Asp Trp Lys Ala Asn His Thr Glu Asp Glu Tyr Ile Arg Tyr 
                245                 250                 255     


Met Gly Ser Phe Tyr Arg Asp 
            260             


<210>  11
<211>  263
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  7-HSDH Mutante

<400>  11

Met Asn Leu Arg Glu Lys Tyr Gly Glu Trp Gly Leu Ile Leu Gly Ala 
1               5                   10                  15      


Thr Glu Gly Val Gly Lys Ala Phe Cys Glu Lys Ile Ala Ala Gly Gly 
            20                  25                  30          


Met Asn Val Val Met Val Asp Phe Arg Glu Glu Lys Leu Asn Val Leu 
        35                  40                  45              


Ala Gly Glu Ile Arg Glu Thr Tyr Gly Val Glu Thr Lys Val Val Arg 
    50                  55                  60                  


Ala Asp Phe Ser Gln Pro Gly Ala Ala Glu Thr Val Phe Ala Ala Thr 
65                  70                  75                  80  


Glu Gly Leu Asp Met Gly Phe Met Ser Tyr Val Ala Cys Leu His Ser 
                85                  90                  95      


Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu Lys His Glu Ala Met Ile 
            100                 105                 110         


Asn Val Asn Val Val Thr Phe Leu Lys Cys Phe His His Tyr Met Arg 
        115                 120                 125             


Ile Phe Ala Ala Gln Asp Arg Gly Ala Val Ile Asn Val Ser Ser Met 
    130                 135                 140                 


Thr Gly Ile Ser Ser Ser Pro Trp Asn Gly Gln Tyr Gly Ala Gly Lys 
145                 150                 155                 160 


Ala Phe Ile Leu Lys Met Thr Glu Ala Val Ala Cys Glu Cys Glu Gly 
                165                 170                 175     


Thr Gly Val Asp Val Glu Val Ile Thr Leu Gly Thr Thr Leu Thr Pro 
            180                 185                 190         


Ser Leu Leu Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu Ala Val Met 
        195                 200                 205             


Lys Ile Ala Leu Thr Pro Glu Glu Cys Val Asp Glu Ala Phe Glu Lys 
    210                 215                 220                 


Leu Gly Lys Glu Leu Ser Val Ile Ala Gly Gln Arg Asn Lys Asp Ser 
225                 230                 235                 240 


Val His Asp Trp Lys Ala Asn His Thr Glu Asp Glu Tyr Ile Arg Tyr 
                245                 250                 255     


Met Gly Ser Phe Tyr Arg Asp 
            260             


<210>  12
<211>  263
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  7-HSDH Mutante

<400>  12

Met Asn Leu Arg Glu Lys Tyr Gly Glu Trp Gly Leu Ile Leu Gly Ala 
1               5                   10                  15      


Thr Glu Gly Val Gly Lys Ala Phe Cys Glu Lys Ile Ala Ala Gly Gly 
            20                  25                  30          


Met Asn Val Val Met Val Asp Phe Lys Glu Glu Lys Leu Asn Val Leu 
        35                  40                  45              


Ala Gly Glu Ile Arg Glu Thr Tyr Gly Val Glu Thr Lys Val Val Arg 
    50                  55                  60                  


Ala Asp Phe Ser Gln Pro Gly Ala Ala Glu Thr Val Phe Ala Ala Thr 
65                  70                  75                  80  


Glu Gly Leu Asp Met Gly Phe Met Ser Tyr Val Ala Cys Leu His Ser 
                85                  90                  95      


Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu Lys His Glu Ala Met Ile 
            100                 105                 110         


Asn Val Asn Val Val Thr Phe Leu Lys Cys Phe His His Tyr Met Arg 
        115                 120                 125             


Ile Phe Ala Ala Gln Asp Arg Gly Ala Val Ile Asn Val Ser Ser Met 
    130                 135                 140                 


Thr Gly Ile Ser Ser Ser Pro Trp Asn Gly Gln Tyr Gly Ala Gly Lys 
145                 150                 155                 160 


Ala Phe Ile Leu Lys Met Thr Glu Ala Val Ala Cys Glu Cys Glu Gly 
                165                 170                 175     


Thr Gly Val Asp Val Glu Val Ile Thr Leu Gly Thr Thr Leu Thr Pro 
            180                 185                 190         


Ser Leu Leu Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu Ala Val Met 
        195                 200                 205             


Lys Ile Ala Leu Thr Pro Glu Glu Cys Val Asp Glu Ala Phe Glu Lys 
    210                 215                 220                 


Leu Gly Lys Glu Leu Ser Val Ile Ala Gly Gln Arg Asn Lys Asp Ser 
225                 230                 235                 240 


Val His Asp Trp Lys Ala Asn His Thr Glu Asp Glu Tyr Ile Arg Tyr 
                245                 250                 255     


Met Gly Ser Phe Tyr Arg Asp 
            260             


<210>  13
<211>  263
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  7-HSDH Mutante

<400>  13

Met Asn Leu Arg Glu Lys Tyr Gly Glu Trp Gly Leu Ile Leu Gly Ala 
1               5                   10                  15      


Thr Glu Gly Val Gly Lys Ala Phe Cys Glu Lys Ile Ala Ala Gly Gly 
            20                  25                  30          


Met Asn Val Val Met Val Asp Phe Lys Glu Glu Gly Leu Asn Val Leu 
        35                  40                  45              


Ala Gly Glu Ile Arg Glu Thr Tyr Gly Val Glu Thr Lys Val Val Arg 
    50                  55                  60                  


Ala Asp Phe Ser Gln Pro Gly Ala Ala Glu Thr Val Phe Ala Ala Thr 
65                  70                  75                  80  


Glu Gly Leu Asp Met Gly Phe Met Ser Tyr Val Ala Cys Leu His Ser 
                85                  90                  95      


Phe Gly Lys Ile Gln Asp Thr Pro Trp Glu Lys His Glu Ala Met Ile 
            100                 105                 110         


Asn Val Asn Val Val Thr Phe Leu Lys Cys Phe His His Tyr Met Arg 
        115                 120                 125             


Ile Phe Ala Ala Gln Asp Arg Gly Ala Val Ile Asn Val Ser Ser Met 
    130                 135                 140                 


Thr Gly Ile Ser Ser Ser Pro Trp Asn Gly Gln Tyr Gly Ala Gly Lys 
145                 150                 155                 160 


Ala Phe Ile Leu Lys Met Thr Glu Ala Val Ala Cys Glu Cys Glu Gly 
                165                 170                 175     


Thr Gly Val Asp Val Glu Val Ile Thr Leu Gly Thr Thr Leu Thr Pro 
            180                 185                 190         


Ser Leu Leu Ser Asn Leu Pro Gly Gly Pro Gln Gly Glu Ala Val Met 
        195                 200                 205             


Lys Ile Ala Leu Thr Pro Glu Glu Cys Val Asp Glu Ala Phe Glu Lys 
    210                 215                 220                 


Leu Gly Lys Glu Leu Ser Val Ile Ala Gly Gln Arg Asn Lys Asp Ser 
225                 230                 235                 240 


Val His Asp Trp Lys Ala Asn His Thr Glu Asp Glu Tyr Ile Arg Tyr 
                245                 250                 255     


Met Gly Ser Phe Tyr Arg Asp 
            260             


<210>  14
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  14
cgtcgtcatg gtcgactttc gcgagg                                            26


<210>  15
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  15
ccgccgcatc cataccgcca gttgtttacc c                                      31


<210>  16
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  16
cgtcgtcatg gtcgacttta aagaggagaa gctg                                   34


<210>  17
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer


<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  n is a, c, g, or t

<400>  17
gtcgacttta aagaggagnd tctgaacgtg ctc                                    33


<210>  18
<211>  7891
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid p7(A)T3rG-A

<400>  18
atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa       60

ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt      120

tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagttaaccg cggcctgcct      180

ggaatgaagg atattgtgtc ataccgccgt ccgcgaataa cgtgatgcct gtgacgtagc      240

tggcttcctt cgaagcaagc caggctgcta ctgcggcgat ctcctccggt tcgccgatat      300

atcccattgg aatcatgctt tctacatcag ctttctgttt agggtcagcg aatttttcag      360

cattgattgg cgtgttgatc gcacctggcc caatattatt gacgcgaatg cccttcggcg      420

cgtattccaa cgctaatgtt tctgtcatca gctttatccc gcctttactt gccgcatagt      480

ggacaaataa cggccaagga atcacttcgt gcacactgga catgttaatg acatttccct      540

tgatatcgtt ttctacgaaa tatttaatcg cttcacggct tcctaaaaag gcacccgtta      600

agttcgtgcc gatgacttta tcccaatcct tgagcggcat ttcgtgagat ggcacaggat      660

tttcaagacc ggcattatta atcataatat cgagtgtgcc gaactcctta attgccgttt      720

gcacgatatt ttttacatct tcctctttcg tgacatctcc ttggacgaca acagcttcac      780

cgcccgcctt gatgacctct tcttttacct cgttcggatc ttgtttatta ctataatagt      840

tgataaccac ttttgcctgc tccttgccga agcgaatggc catcgccttt ccgagccctg      900

aagcagctcc tgtaatagcg acgacttttc cttttaaatc cggatacatg tatatctcct      960

tgcggccgct cagaactgtg tcgggcgcat caccgcatca atgccgccat caatgacgat     1020

ctgcgcgcca tgcacatagc ttgcggccgg gctcatcaaa aaggcgatga ccgacgccat     1080

ctcggacggc tcggcacggc ggcccatggg aggaacgaac ttggcaatgg attcgccata     1140

gcgcgggtcc tgcaggcccg cctgcagcaa gggagtctcg gttgcaccgg gggcgatggt     1200

gttcaggcgc acgccagcct cgccccaggc ggcggcgcgt ttgcgcacag ccaccgtcaa     1260

agcattcttg ctgcccgcat aggccagatt tccgccctgc tctcccgcat gttcgacaat     1320

ggcgcgggcc ttggcttcct cgccggcttc cagtgccagc gccagtgggt tcttgtcaaa     1380

agccagatgc gcggaagcca cggacgagat gacgacggct gcgggctgat ggcctttttt     1440

cagcgctggc aaaaaggcat ccatcagctc ggtcgcgcca aaataattga ccgaaaccac     1500

attgccaagc accttggtct gcggtcccag gccggcgcac agcaccaggc cgtccatgcc     1560

cttgctgcac ttcgccagta catcggcaat cgcctgcttt cgaccttcgg ccgtcgagag     1620

atcggcaatc acttccgcat cgcgtatatc gatgcctacg atctggtgac cggccgcctc     1680

caggaccttg cgcgtagcgg caccaatgcc ggtggcgcag ccgcttatca cgatgatgga     1740

catcatatgt atatctcctt cttatactta actaatatac taagatgggg aattgttatc     1800

cgctcacaat tcccctatag tgagtcgtat taatttcgat tatgcggccg tgtacaatac     1860

gattactttc tgttcgactt aagcattata agcttctagt cgcggtagaa cgaccccatg     1920

tagcggatgt actcgtcctc ggtgtggttt gccttccagt cgtggacgga gtccttgttg     1980

cgctggccgg cgatgacgga gagctcctta cccagcttct caaaggcctc gtcaacgcac     2040

tcctcggggg tgagggcgat cttcatgacg gcctcgccct gcgggccgcc ggggaggttg     2100

gacagcaggc tgggggttag ggtggtgccg agggtgatga cctcgacgtc gacgccggtg     2160

ccctcgcact cgcaggccac ggcctcggtc atcttgagga tgaaggcctt gcccgcgccg     2220

tactggccgt tccaggggct ggagctgatg ccggtcatcg acgagacgtt gatcacggcg     2280

ccgcggtcct gggcggcaaa gatccgcatg tagtggtgga agcacttgag gaaggtcacg     2340

acgttgacgt tgatcatggc ctcgtgcttc tcccaggggg tgtcctggat cttaccgaag     2400

ctgtgcaggc aggccacgta gctcatgaag cccatgtcca ggccctcggt cgcggcgaag     2460

acggtctcgg cagcgccggg ctggctaaag tcggcgcgca cgaccttggt ctccacgccg     2520

taggtctcgc ggatctcgcc tgcgagcacg ttcagcttct cctcgcgacg ggcgaccatg     2580

acgacgttca tgccgccggc ggcgatcttc tcgcagaacg ccttgccgac gccctcggtc     2640

gcgcccagga tcaggcccca ctcaccgtac ttctccctca ggttcatatg tatatctcct     2700

tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac aattccccta     2760

tagtgagtcg tattaatttc gcgggatcga gatctcgatc ctctacgccg gacgcatcgt     2820

ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga     2880

tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt     2940

ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc     3000

ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca     3060

taagggagag cgtcgagatc ccggacacca tcgaatggcg caaaaccttt cgcggtatgg     3120

catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat     3180

acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg     3240

ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt     3300

acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg     3360

ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg     3420

ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct     3480

gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc     3540

cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat     3600

ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat gaagacggta     3660

cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg     3720

gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc     3780

gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc     3840

aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg     3900

atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg     3960

atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa     4020

ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac     4080

tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa     4140

aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa     4200

tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat     4260

gtaagttagc tcactcatta ggcaccggga tctcgaccga tgcccttgag agccttcaac     4320

ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc     4380

ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag     4440

gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg     4500

cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag     4560

gccattatcg ccggcatggc ggccccacgg gtgcgcatga tcgtgctcct gtcgttgagg     4620

acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag     4680

cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc     4740

ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg     4800

ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta     4860

acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc     4920

agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc     4980

gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac     5040

acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga     5100

agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac     5160

atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc     5220

ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg     5280

taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt     5340

cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg     5400

cggcatcaga gcagattgta ctgagagtgc accatatatg cggtgtgaaa taccgcacag     5460

atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct     5520

gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt     5580

atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc     5640

caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga     5700

gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata     5760

ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac     5820

cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg     5880

taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc     5940

cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag     6000

acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt     6060

aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt     6120

atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg     6180

atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac     6240

gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca     6300

gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac     6360

ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac     6420

ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt     6480

tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt     6540

accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt     6600

atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc     6660

cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa     6720

tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg     6780

tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt     6840

gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc     6900

agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt     6960

aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg     7020

gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac     7080

tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc     7140

gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt     7200

tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg     7260

aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag     7320

catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa     7380

acaaataggg gttccgcgca catttccccg aaaagtgcca cctgaaattg taaacgttaa     7440

tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc     7500

cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt     7560

tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa     7620

aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg     7680

gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg     7740

acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc     7800

tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa     7860

tgcgccgcta cagggcgcgt cccattcgcc a                                    7891


<210>  19
<211>  7758
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid p7(A)T3rG-K

<400>  19
atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa       60

ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt      120

tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagttaaccg cggcctgcct      180

ggaatgaagg atattgtgtc ataccgccgt ccgcgaataa cgtgatgcct gtgacgtagc      240

tggcttcctt cgaagcaagc caggctgcta ctgcggcgat ctcctccggt tcgccgatat      300

atcccattgg aatcatgctt tctacatcag ctttctgttt agggtcagcg aatttttcag      360

cattgattgg cgtgttgatc gcacctggcc caatattatt gacgcgaatg cccttcggcg      420

cgtattccaa cgctaatgtt tctgtcatca gctttatccc gcctttactt gccgcatagt      480

ggacaaataa cggccaagga atcacttcgt gcacactgga catgttaatg acatttccct      540

tgatatcgtt ttctacgaaa tatttaatcg cttcacggct tcctaaaaag gcacccgtta      600

agttcgtgcc gatgacttta tcccaatcct tgagcggcat ttcgtgagat ggcacaggat      660

tttcaagacc ggcattatta atcataatat cgagtgtgcc gaactcctta attgccgttt      720

gcacgatatt ttttacatct tcctctttcg tgacatctcc ttggacgaca acagcttcac      780

cgcccgcctt gatgacctct tcttttacct cgttcggatc ttgtttatta ctataatagt      840

tgataaccac ttttgcctgc tccttgccga agcgaatggc catcgccttt ccgagccctg      900

aagcagctcc tgtaatagcg acgacttttc cttttaaatc cggatacatg tatatctcct      960

tgcggccgct cagaactgtg tcgggcgcat caccgcatca atgccgccat caatgacgat     1020

ctgcgcgcca tgcacatagc ttgcggccgg gctcatcaaa aaggcgatga ccgacgccat     1080

ctcggacggc tcggcacggc ggcccatggg aggaacgaac ttggcaatgg attcgccata     1140

gcgcgggtcc tgcaggcccg cctgcagcaa gggagtctcg gttgcaccgg gggcgatggt     1200

gttcaggcgc acgccagcct cgccccaggc ggcggcgcgt ttgcgcacag ccaccgtcaa     1260

agcattcttg ctgcccgcat aggccagatt tccgccctgc tctcccgcat gttcgacaat     1320

ggcgcgggcc ttggcttcct cgccggcttc cagtgccagc gccagtgggt tcttgtcaaa     1380

agccagatgc gcggaagcca cggacgagat gacgacggct gcgggctgat ggcctttttt     1440

cagcgctggc aaaaaggcat ccatcagctc ggtcgcgcca aaataattga ccgaaaccac     1500

attgccaagc accttggtct gcggtcccag gccggcgcac agcaccaggc cgtccatgcc     1560

cttgctgcac ttcgccagta catcggcaat cgcctgcttt cgaccttcgg ccgtcgagag     1620

atcggcaatc acttccgcat cgcgtatatc gatgcctacg atctggtgac cggccgcctc     1680

caggaccttg cgcgtagcgg caccaatgcc ggtggcgcag ccgcttatca cgatgatgga     1740

catcatatgt atatctcctt cttatactta actaatatac taagatgggg aattgttatc     1800

cgctcacaat tcccctatag tgagtcgtat taatttcgat tatgcggccg tgtacaatac     1860

gattactttc tgttcgactt aagcattata agcttctagt cgcggtagaa cgaccccatg     1920

tagcggatgt actcgtcctc ggtgtggttt gccttccagt cgtggacgga gtccttgttg     1980

cgctggccgg cgatgacgga gagctcctta cccagcttct caaaggcctc gtcaacgcac     2040

tcctcggggg tgagggcgat cttcatgacg gcctcgccct gcgggccgcc ggggaggttg     2100

gacagcaggc tgggggttag ggtggtgccg agggtgatga cctcgacgtc gacgccggtg     2160

ccctcgcact cgcaggccac ggcctcggtc atcttgagga tgaaggcctt gcccgcgccg     2220

tactggccgt tccaggggct ggagctgatg ccggtcatcg acgagacgtt gatcacggcg     2280

ccgcggtcct gggcggcaaa gatccgcatg tagtggtgga agcacttgag gaaggtcacg     2340

acgttgacgt tgatcatggc ctcgtgcttc tcccaggggg tgtcctggat cttaccgaag     2400

ctgtgcaggc aggccacgta gctcatgaag cccatgtcca ggccctcggt cgcggcgaag     2460

acggtctcgg cagcgccggg ctggctaaag tcggcgcgca cgaccttggt ctccacgccg     2520

taggtctcgc ggatctcgcc tgcgagcacg ttcagcttct cctcgcgacg ggcgaccatg     2580

acgacgttca tgccgccggc ggcgatcttc tcgcagaacg ccttgccgac gccctcggtc     2640

gcgcccagga tcaggcccca ctcaccgtac ttctccctca ggttcatatg tatatctcct     2700

tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac aattccccta     2760

tagtgagtcg tattaatttc gcgggatcga gatctcgatc ctctacgccg gacgcatcgt     2820

ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga     2880

tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt     2940

ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc     3000

ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca     3060

taagggagag cgtcgagatc ccggacacca tcgaatggcg caaaaccttt cgcggtatgg     3120

catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat     3180

acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg     3240

ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt     3300

acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg     3360

ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg     3420

ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct     3480

gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc     3540

cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat     3600

ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat gaagacggta     3660

cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg     3720

gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc     3780

gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc     3840

aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg     3900

atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg     3960

atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc ccgccgttaa     4020

ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac     4080

tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa     4140

aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa     4200

tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat     4260

gtaagttagc tcactcatta ggcaccggga tctcgaccga tgcccttgag agccttcaac     4320

ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc     4380

ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat tttcggcgag     4440

gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg     4500

cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag     4560

gccattatcg ccggcatggc ggccccacgg gtgcgcatga tcgtgctcct gtcgttgagg     4620

acccggctag gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag     4680

cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc     4740

ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg     4800

ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta     4860

acgaagcgct ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc     4920

agttgtttac cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc     4980

gtgagcatcc tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac     5040

acggaggcat cagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga     5100

agccagacat taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac     5160

atctgtgaat cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc     5220

ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg     5280

taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt     5340

cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg     5400

cggcatcaga gcagattgta ctgagagtgc accatatatg cggtgtgaaa taccgcacag     5460

atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct     5520

gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt     5580

atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc     5640

caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga     5700

gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata     5760

ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac     5820

cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg     5880

taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc     5940

cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag     6000

acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt     6060

aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt     6120

atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg     6180

atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac     6240

gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca     6300

gtggaacgaa aactcacgtt aagggatttt ggtcatgaac aataaaactg tctgcttaca     6360

taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct tgctctaggc     6420

cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg     6480

tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt     6540

ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa     6600

actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg     6660

atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta ttagaagaat     6720

atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt     6780

cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc     6840

aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct     6900

ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag     6960

tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag     7020

gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat     7080

ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta     7140

ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaag     7200

aattaattca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt     7260

ccgcgcacat ttccccgaaa agtgccacct gaaattgtaa acgttaatat tttgttaaaa     7320

ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa     7380

atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac     7440

aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag     7500

ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt     7560

aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg     7620

gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca     7680

agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag     7740

ggcgcgtccc attcgcca                                                   7758


<210>  20
<211>  8019
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid p7(A)T3TG-A

<400>  20
atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa       60

ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt      120

tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagttaaccg cggcctgcct      180

ggaatgaagg atattgtgtc ataccgccgt ccgcgaataa cgtgatgcct gtgacgtagc      240

tggcttcctt cgaagcaagc caggctgcta ctgcggcgat ctcctccggt tcgccgatat      300

atcccattgg aatcatgctt tctacatcag ctttctgttt agggtcagcg aatttttcag      360

cattgattgg cgtgttgatc gcacctggcc caatattatt gacgcgaatg cccttcggcg      420

cgtattccaa cgctaatgtt tctgtcatca gctttatccc gcctttactt gccgcatagt      480

ggacaaataa cggccaagga atcacttcgt gcacactgga catgttaatg acatttccct      540

tgatatcgtt ttctacgaaa tatttaatcg cttcacggct tcctaaaaag gcacccgtta      600

agttcgtgcc gatgacttta tcccaatcct tgagcggcat ttcgtgagat ggcacaggat      660

tttcaagacc ggcattatta atcataatat cgagtgtgcc gaactcctta attgccgttt      720

gcacgatatt ttttacatct tcctctttcg tgacatctcc ttggacgaca acagcttcac      780

cgcccgcctt gatgacctct tcttttacct cgttcggatc ttgtttatta ctataatagt      840

tgataaccac ttttgcctgc tccttgccga agcgaatggc catcgccttt ccgagccctg      900

aagcagctcc tgtaatagcg acgacttttc cttttaaatc cggatacata tgtatatctc      960

cttcttatac ttaactaata tactaagatg gggaattgtt atccgctcac aattccccta     1020

tagtgagtcg tattaatttc gattatgcgg ccgtgtacaa tacgattact ttctgttcga     1080

cttaagcatt atgcggccgc tcagaactgt gtcgggcgca tcaccgcatc aatgccgcca     1140

tcaatgacga tctgcgcgcc atgcacatag cttgcggccg ggctcatcaa aaaggcgatg     1200

accgacgcca tctcggacgg ctcggcacgg cggcccatgg gaggaacgaa cttggcaatg     1260

gattcgccat agcgcgggtc ctgcaggccc gcctgcagca agggagtctc ggttgcaccg     1320

ggggcgatgg tgttcaggcg cacgccagcc tcgccccagg cggcggcgcg tttgcgcaca     1380

gccaccgtca aagcattctt gctgcccgca taggccagat ttccgccctg ctctcccgca     1440

tgttcgacaa tggcgcgggc cttggcttcc tcgccggctt ccagtgccag cgccagtggg     1500

ttcttgtcaa aagccagatg cgcggaagcc acggacgaga tgacgacggc tgcgggctga     1560

tggccttttt tcagcgctgg caaaaaggca tccatcagct cggtcgcgcc aaaataattg     1620

accgaaacca cattgccaag caccttggtc tgcggtccca ggccggcgca cagcaccagg     1680

ccgtccatgc ccttgctgca cttcgccagt acatcggcaa tcgcctgctt tcgaccttcg     1740

gccgtcgaga gatcggcaat cacttccgca tcgcgtatat cgatgcctac gatctggtga     1800

ccggccgcct ccaggacctt gcgcgtagcg gcaccaatgc cggtggcgca gccgcttatc     1860

acgatgatgg acatatgtat atctccttct tatacttaac taatatacta agatggggaa     1920

ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcgatta tgcggccgtg     1980

tacaatacga ttactttctg ttcgacttaa gcattataag cttctagtcg cggtagaacg     2040

accccatgta gcggatgtac tcgtcctcgg tgtggtttgc cttccagtcg tggacggagt     2100

ccttgttgcg ctggccggcg atgacggaga gctccttacc cagcttctca aaggcctcgt     2160

caacgcactc ctcgggggtg agggcgatct tcatgacggc ctcgccctgc gggccgccgg     2220

ggaggttgga cagcaggctg ggggttaggg tggtgccgag ggtgatgacc tcgacgtcga     2280

cgccggtgcc ctcgcactcg caggccacgg cctcggtcat cttgaggatg aaggccttgc     2340

ccgcgccgta ctggccgttc caggggctgg agctgatgcc ggtcatcgac gagacgttga     2400

tcacggcgcc gcggtcctgg gcggcaaaga tccgcatgta gtggtggaag cacttgagga     2460

aggtcacgac gttgacgttg atcatggcct cgtgcttctc ccagggggtg tcctggatct     2520

taccgaagct gtgcaggcag gccacgtagc tcatgaagcc catgtccagg ccctcggtcg     2580

cggcgaagac ggtctcggca gcgccgggct ggctaaagtc ggcgcgcacg accttggtct     2640

ccacgccgta ggtctcgcgg atctcgcctg cgagcacgtt cagcttctcc tcgcgacggg     2700

cgaccatgac gacgttcatg ccgccggcgg cgatcttctc gcagaacgcc ttgccgacgc     2760

cctcggtcgc gcccaggatc aggccccact caccgtactt ctccctcagg ttcatatgta     2820

tatctccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat ccgctcacaa     2880

ttcccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct ctacgccgga     2940

cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac     3000

atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg     3060

ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca     3120

ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag     3180

gagtcgcata agggagagcg tcgagatccc ggacaccatc gaatggcgca aaacctttcg     3240

cggtatggca tgatagcgcc cggaagagag tcaattcagg gtggtgaatg tgaaaccagt     3300

aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt     3360

gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga     3420

gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat     3480

tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa     3540

atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt     3600

cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat     3660

taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc     3720

ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga     3780

agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct     3840

gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata     3900

tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc     3960

cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt     4020

tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt     4080

tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc     4140

gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt     4200

gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt     4260

gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga     4320

ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg     4380

caattaatgt aagttagctc actcattagg caccgggatc tcgaccgatg cccttgagag     4440

ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta     4500

tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt     4560

tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg     4620

gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg     4680

agaagcaggc cattatcgcc ggcatggcgg ccccacgggt gcgcatgatc gtgctcctgt     4740

cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga     4800

tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat     4860

gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca     4920

ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat     4980

ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc     5040

ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa     5100

cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatc     5160

ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac atggcccgct     5220

ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac     5280

aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc agctgcctcg     5340

cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag     5400

cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg     5460

gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct     5520

taactatgcg gcatcagagc agattgtact gagagtgcac catatatgcg gtgtgaaata     5580

ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact     5640

gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta     5700

atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag     5760

caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc     5820

cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta     5880

taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg     5940

ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc     6000

tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac     6060

gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac     6120

ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg     6180

aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga     6240

aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt     6300

agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag     6360

cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct     6420

gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg     6480

atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat     6540

gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc     6600

tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg     6660

gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct     6720

ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca     6780

actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg     6840

ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg     6900

tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc     6960

cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag     7020

ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg     7080

ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag     7140

tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat     7200

agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg     7260

atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca     7320

gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca     7380

aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat     7440

tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag     7500

aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaaattgta     7560

aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac     7620

caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg     7680

agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa     7740

gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt     7800

tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt     7860

agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga     7920

gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc     7980

gcgcttaatg cgccgctaca gggcgcgtcc cattcgcca                            8019


<210>  21
<211>  7886
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid p7(A)T3TG-K

<400>  21
atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa       60

ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt      120

tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagttaaccg cggcctgcct      180

ggaatgaagg atattgtgtc ataccgccgt ccgcgaataa cgtgatgcct gtgacgtagc      240

tggcttcctt cgaagcaagc caggctgcta ctgcggcgat ctcctccggt tcgccgatat      300

atcccattgg aatcatgctt tctacatcag ctttctgttt agggtcagcg aatttttcag      360

cattgattgg cgtgttgatc gcacctggcc caatattatt gacgcgaatg cccttcggcg      420

cgtattccaa cgctaatgtt tctgtcatca gctttatccc gcctttactt gccgcatagt      480

ggacaaataa cggccaagga atcacttcgt gcacactgga catgttaatg acatttccct      540

tgatatcgtt ttctacgaaa tatttaatcg cttcacggct tcctaaaaag gcacccgtta      600

agttcgtgcc gatgacttta tcccaatcct tgagcggcat ttcgtgagat ggcacaggat      660

tttcaagacc ggcattatta atcataatat cgagtgtgcc gaactcctta attgccgttt      720

gcacgatatt ttttacatct tcctctttcg tgacatctcc ttggacgaca acagcttcac      780

cgcccgcctt gatgacctct tcttttacct cgttcggatc ttgtttatta ctataatagt      840

tgataaccac ttttgcctgc tccttgccga agcgaatggc catcgccttt ccgagccctg      900

aagcagctcc tgtaatagcg acgacttttc cttttaaatc cggatacata tgtatatctc      960

cttcttatac ttaactaata tactaagatg gggaattgtt atccgctcac aattccccta     1020

tagtgagtcg tattaatttc gattatgcgg ccgtgtacaa tacgattact ttctgttcga     1080

cttaagcatt atgcggccgc tcagaactgt gtcgggcgca tcaccgcatc aatgccgcca     1140

tcaatgacga tctgcgcgcc atgcacatag cttgcggccg ggctcatcaa aaaggcgatg     1200

accgacgcca tctcggacgg ctcggcacgg cggcccatgg gaggaacgaa cttggcaatg     1260

gattcgccat agcgcgggtc ctgcaggccc gcctgcagca agggagtctc ggttgcaccg     1320

ggggcgatgg tgttcaggcg cacgccagcc tcgccccagg cggcggcgcg tttgcgcaca     1380

gccaccgtca aagcattctt gctgcccgca taggccagat ttccgccctg ctctcccgca     1440

tgttcgacaa tggcgcgggc cttggcttcc tcgccggctt ccagtgccag cgccagtggg     1500

ttcttgtcaa aagccagatg cgcggaagcc acggacgaga tgacgacggc tgcgggctga     1560

tggccttttt tcagcgctgg caaaaaggca tccatcagct cggtcgcgcc aaaataattg     1620

accgaaacca cattgccaag caccttggtc tgcggtccca ggccggcgca cagcaccagg     1680

ccgtccatgc ccttgctgca cttcgccagt acatcggcaa tcgcctgctt tcgaccttcg     1740

gccgtcgaga gatcggcaat cacttccgca tcgcgtatat cgatgcctac gatctggtga     1800

ccggccgcct ccaggacctt gcgcgtagcg gcaccaatgc cggtggcgca gccgcttatc     1860

acgatgatgg acatatgtat atctccttct tatacttaac taatatacta agatggggaa     1920

ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcgatta tgcggccgtg     1980

tacaatacga ttactttctg ttcgacttaa gcattataag cttctagtcg cggtagaacg     2040

accccatgta gcggatgtac tcgtcctcgg tgtggtttgc cttccagtcg tggacggagt     2100

ccttgttgcg ctggccggcg atgacggaga gctccttacc cagcttctca aaggcctcgt     2160

caacgcactc ctcgggggtg agggcgatct tcatgacggc ctcgccctgc gggccgccgg     2220

ggaggttgga cagcaggctg ggggttaggg tggtgccgag ggtgatgacc tcgacgtcga     2280

cgccggtgcc ctcgcactcg caggccacgg cctcggtcat cttgaggatg aaggccttgc     2340

ccgcgccgta ctggccgttc caggggctgg agctgatgcc ggtcatcgac gagacgttga     2400

tcacggcgcc gcggtcctgg gcggcaaaga tccgcatgta gtggtggaag cacttgagga     2460

aggtcacgac gttgacgttg atcatggcct cgtgcttctc ccagggggtg tcctggatct     2520

taccgaagct gtgcaggcag gccacgtagc tcatgaagcc catgtccagg ccctcggtcg     2580

cggcgaagac ggtctcggca gcgccgggct ggctaaagtc ggcgcgcacg accttggtct     2640

ccacgccgta ggtctcgcgg atctcgcctg cgagcacgtt cagcttctcc tcgcgacggg     2700

cgaccatgac gacgttcatg ccgccggcgg cgatcttctc gcagaacgcc ttgccgacgc     2760

cctcggtcgc gcccaggatc aggccccact caccgtactt ctccctcagg ttcatatgta     2820

tatctccttc ttaaagttaa acaaaattat ttctagaggg gaattgttat ccgctcacaa     2880

ttcccctata gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct ctacgccgga     2940

cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac     3000

atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg     3060

ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca     3120

ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag     3180

gagtcgcata agggagagcg tcgagatccc ggacaccatc gaatggcgca aaacctttcg     3240

cggtatggca tgatagcgcc cggaagagag tcaattcagg gtggtgaatg tgaaaccagt     3300

aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt     3360

gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga     3420

gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat     3480

tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa     3540

atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt     3600

cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat     3660

taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc     3720

ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga     3780

agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct     3840

gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata     3900

tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc     3960

cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt     4020

tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt     4080

tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc     4140

gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt     4200

gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt     4260

gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga     4320

ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg     4380

caattaatgt aagttagctc actcattagg caccgggatc tcgaccgatg cccttgagag     4440

ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta     4500

tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt     4560

tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg     4620

gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg     4680

agaagcaggc cattatcgcc ggcatggcgg ccccacgggt gcgcatgatc gtgctcctgt     4740

cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga     4800

tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat     4860

gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca     4920

ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat     4980

ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc     5040

ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa     5100

cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatc     5160

ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac atggcccgct     5220

ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac     5280

aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc agctgcctcg     5340

cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag     5400

cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg     5460

gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct     5520

taactatgcg gcatcagagc agattgtact gagagtgcac catatatgcg gtgtgaaata     5580

ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact     5640

gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta     5700

atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag     5760

caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc     5820

cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta     5880

taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg     5940

ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc     6000

tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac     6060

gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac     6120

ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg     6180

aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga     6240

aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt     6300

agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag     6360

cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct     6420

gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgaacaa taaaactgtc     6480

tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcttg     6540

ctctaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg     6600

cgataatgtc gggcaatcag gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc     6660

agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt     6720

cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac     6780

tcctgatgat gcatggttac tcaccactgc gatccccggg aaaacagcat tccaggtatt     6840

agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg     6900

gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc     6960

tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg     7020

taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc     7080

ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa     7140

attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc     7200

catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa     7260

atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt     7320

tttctaagaa ttaattcatg agcggataca tatttgaatg tatttagaaa aataaacaaa     7380

taggggttcc gcgcacattt ccccgaaaag tgccacctga aattgtaaac gttaatattt     7440

tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa     7500

tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag     7560

tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg     7620

tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga     7680

ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg     7740

gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg     7800

cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc     7860

cgctacaggg cgcgtcccat tcgcca                                          7886


