                         SEQUENCE LISTING

<110>  The Trustees of the University of Pennsylvania
 
<120>  Apparatus and Methods for Making Recombinant Protein-Stabilized 
       Monodisperse Microbubbles

<130>  UOP-510WO

<150>  US 62/014,451
<151>  2014-06-19

<160>  23    

<170>  PatentIn version 3.5

<210>  1
<211>  140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Oleosin Variant

<400>  1

Gly Ser Ala Thr Thr Thr Tyr Asp Arg His His Val Thr Thr Thr Gln 
1               5                   10                  15      


Pro Gln Tyr Arg His Asp Gln His Thr Gly Asp Arg Leu Thr His Pro 
            20                  25                  30          


Gln Arg Gln Gln Gln Gly Pro Ser Thr Gly Lys Leu Ala Leu Gly Ala 
        35                  40                  45              


Thr Pro Leu Phe Gly Val Ile Gly Phe Ser Pro Val Ile Val Pro Ala 
    50                  55                  60                  


Met Gly Ile Ala Ile Gly Leu Ala Gly Val Thr Gly Phe Gln Arg Asp 
65                  70                  75                  80  


Tyr Val Lys Gly Lys Leu Gln Asp Val Gly Glu Tyr Thr Gly Gln Lys 
                85                  90                  95      


Thr Lys Asp Leu Gly Gln Lys Ile Gln His Thr Ala His Glu Met Gly 
            100                 105                 110         


Asp Gln Gly Gln Gly Gln Gly Gln Gly Gly Gly Lys Glu Gly Arg Lys 
        115                 120                 125             


Glu Gly Gly Lys Leu Glu His His His His His His 
    130                 135                 140 


<210>  2
<211>  378
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Oleosin Variant

<400>  2

Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 
    50                  55                  60                  


Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly 
145                 150                 155                 160 


Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val 
                165                 170                 175     


Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro 
            180                 185                 190         


Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser 
        195                 200                 205             


Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val 
    210                 215                 220                 


Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys Gly Ser 
225                 230                 235                 240 


Ala Thr Thr Thr Tyr Asp Arg His His Val Thr Thr Thr Gln Pro Gln 
                245                 250                 255     


Tyr Arg His Asp Gln His Thr Gly Asp Arg Leu Thr His Pro Gln Arg 
            260                 265                 270         


Gln Gln Gln Gly Pro Ser Thr Gly Lys Leu Ala Leu Gly Ala Thr Pro 
        275                 280                 285             


Leu Phe Gly Val Ile Gly Phe Ser Pro Val Ile Val Pro Ala Met Gly 
    290                 295                 300                 


Ile Ala Ile Gly Leu Ala Gly Val Thr Gly Phe Gln Arg Asp Tyr Val 
305                 310                 315                 320 


Lys Gly Lys Leu Gln Asp Val Gly Glu Tyr Thr Gly Gln Lys Thr Lys 
                325                 330                 335     


Asp Leu Gly Gln Lys Ile Gln His Thr Ala His Glu Met Gly Asp Gln 
            340                 345                 350         


Gly Gln Gly Gln Gly Gln Gly Gly Gly Lys Glu Gly Arg Lys Glu Gly 
        355                 360                 365             


Gly Lys Leu Glu His His His His His His 
    370                 375             


<210>  3
<211>  192
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Oleosin Variant

<400>  3

Met Ala Thr Thr Thr Tyr Asp Arg His His Val Thr Thr Thr Gln Pro 
1               5                   10                  15      


Gln Tyr Arg His Asp Gln His Thr Gly Asp Arg Leu Thr His Pro Gln 
            20                  25                  30          


Arg Gln Gln Gln Gly Pro Ser Thr Gly Lys Ile Met Val Ile Met Ala 
        35                  40                  45              


Leu Leu Pro Ile Thr Gly Ile Leu Phe Gly Leu Ala Gly Ile Thr Leu 
    50                  55                  60                  


Val Gly Thr Val Ile Gly Leu Ala Leu Ala Thr Pro Leu Phe Val Ile 
65                  70                  75                  80  


Phe Ser Pro Val Ile Val Pro Ala Met Ile Ala Ile Gly Leu Ala Val 
                85                  90                  95      


Thr Gly Phe Leu Thr Ser Gly Thr Phe Gly Leu Thr Gly Leu Ser Ser 
            100                 105                 110         


Leu Ser Tyr Leu Phe Asn Met Val Arg Arg Ser Thr Met Ser Val Pro 
        115                 120                 125             


Val Gln Arg Asp Tyr Val Lys Gly Lys Leu Gln Asp Val Gly Glu Tyr 
    130                 135                 140                 


Thr Gly Gln Lys Thr Lys Asp Leu Gly Gln Lys Ile Gln His Thr Ala 
145                 150                 155                 160 


His Glu Met Gly Asp Gln Gly Gln Gly Gln Gly Gln Gly Gly Gly Lys 
                165                 170                 175     


Glu Gly Arg Lys Glu Gly Gly Lys Leu Glu His His His His His His 
            180                 185                 190         


<210>  4
<211>  192
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Oleosin Variant


<220>
<221>  MISC_FEATURE
<222>  (43)..(129)
<223>  Xaa at positions 43 to 129 may be any naturally-occurring or 
       artificial amino acid and up to 87 of them may be absent

<400>  4

Met Ala Thr Thr Thr Tyr Asp Arg His His Val Thr Thr Thr Gln Pro 
1               5                   10                  15      


Gln Tyr Arg His Asp Gln His Thr Gly Asp Arg Leu Thr His Pro Gln 
            20                  25                  30          


Arg Gln Gln Gln Gly Pro Ser Thr Gly Lys Xaa Xaa Xaa Xaa Xaa Xaa 
        35                  40                  45              


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
    50                  55                  60                  


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
65                  70                  75                  80  


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
                85                  90                  95      


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
            100                 105                 110         


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        115                 120                 125             


Xaa Gln Arg Asp Tyr Val Lys Gly Lys Leu Gln Asp Val Gly Glu Tyr 
    130                 135                 140                 


Thr Gly Gln Lys Thr Lys Asp Leu Gly Gln Lys Ile Gln His Thr Ala 
145                 150                 155                 160 


His Glu Met Gly Asp Gln Gly Gln Gly Gln Gly Gln Gly Gly Gly Lys 
                165                 170                 175     


Glu Gly Arg Lys Glu Gly Gly Lys Leu Glu His His His His His His 
            180                 185                 190         


<210>  5
<211>  192
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Oleosin Variant


<220>
<221>  MISC_FEATURE
<222>  (1)..(42)
<223>  Xaa at positions 1 to 42 may be any naturally-occurring or 
       artificial amino acid and up to 42 of them may be absent

<400>  5

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10                  15      


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
            20                  25                  30          


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ile Met Val Ile Met Ala 
        35                  40                  45              


Leu Leu Pro Ile Thr Gly Ile Leu Phe Gly Leu Ala Gly Ile Thr Leu 
    50                  55                  60                  


Val Gly Thr Val Ile Gly Leu Ala Leu Ala Thr Pro Leu Phe Val Ile 
65                  70                  75                  80  


Phe Ser Pro Val Ile Val Pro Ala Met Ile Ala Ile Gly Leu Ala Val 
                85                  90                  95      


Thr Gly Phe Leu Thr Ser Gly Thr Phe Gly Leu Thr Gly Leu Ser Ser 
            100                 105                 110         


Leu Ser Tyr Leu Phe Asn Met Val Arg Arg Ser Thr Met Ser Val Pro 
        115                 120                 125             


Val Gln Arg Asp Tyr Val Lys Gly Lys Leu Gln Asp Val Gly Glu Tyr 
    130                 135                 140                 


Thr Gly Gln Lys Thr Lys Asp Leu Gly Gln Lys Ile Gln His Thr Ala 
145                 150                 155                 160 


His Glu Met Gly Asp Gln Gly Gln Gly Gln Gly Gln Gly Gly Gly Lys 
                165                 170                 175     


Glu Gly Arg Lys Glu Gly Gly Lys Leu Glu His His His His His His 
            180                 185                 190         


<210>  6
<211>  192
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Oleosin Variant


<220>
<221>  MISC_FEATURE
<222>  (130)..(192)
<223>  Xaa at positions 130 to 192 may be any naturally-occurring or 
       artificial amino acid and up to 63 of them may be absent

<400>  6

Met Ala Thr Thr Thr Tyr Asp Arg His His Val Thr Thr Thr Gln Pro 
1               5                   10                  15      


Gln Tyr Arg His Asp Gln His Thr Gly Asp Arg Leu Thr His Pro Gln 
            20                  25                  30          


Arg Gln Gln Gln Gly Pro Ser Thr Gly Lys Ile Met Val Ile Met Ala 
        35                  40                  45              


Leu Leu Pro Ile Thr Gly Ile Leu Phe Gly Leu Ala Gly Ile Thr Leu 
    50                  55                  60                  


Val Gly Thr Val Ile Gly Leu Ala Leu Ala Thr Pro Leu Phe Val Ile 
65                  70                  75                  80  


Phe Ser Pro Val Ile Val Pro Ala Met Ile Ala Ile Gly Leu Ala Val 
                85                  90                  95      


Thr Gly Phe Leu Thr Ser Gly Thr Phe Gly Leu Thr Gly Leu Ser Ser 
            100                 105                 110         


Leu Ser Tyr Leu Phe Asn Met Val Arg Arg Ser Thr Met Ser Val Pro 
        115                 120                 125             


Val Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
    130                 135                 140                 


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
145                 150                 155                 160 


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
                165                 170                 175     


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
            180                 185                 190         


<210>  7
<211>  192
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Oleosin Variant


<220>
<221>  MISC_FEATURE
<222>  (1)..(129)
<223>  Xaa at positions 1 to 129 may be any naturally-occurring or 
       artificial amino acid and up to 129 of them may be absent

<400>  7

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10                  15      


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
            20                  25                  30          


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        35                  40                  45              


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
    50                  55                  60                  


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
65                  70                  75                  80  


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
                85                  90                  95      


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
            100                 105                 110         


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        115                 120                 125             


Xaa Gln Arg Asp Tyr Val Lys Gly Lys Leu Gln Asp Val Gly Glu Tyr 
    130                 135                 140                 


Thr Gly Gln Lys Thr Lys Asp Leu Gly Gln Lys Ile Gln His Thr Ala 
145                 150                 155                 160 


His Glu Met Gly Asp Gln Gly Gln Gly Gln Gly Gln Gly Gly Gly Lys 
                165                 170                 175     


Glu Gly Arg Lys Glu Gly Gly Lys Leu Glu His His His His His His 
            180                 185                 190         


<210>  8
<211>  192
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Oleosin Variant


<220>
<221>  MISC_FEATURE
<222>  (43)..(192)
<223>  Xaa at positions 43 to 192 may be any naturally-occurring or 
       artificial amino acid and up to 150 of them may be absent

<400>  8

Met Ala Thr Thr Thr Tyr Asp Arg His His Val Thr Thr Thr Gln Pro 
1               5                   10                  15      


Gln Tyr Arg His Asp Gln His Thr Gly Asp Arg Leu Thr His Pro Gln 
            20                  25                  30          


Arg Gln Gln Gln Gly Pro Ser Thr Gly Lys Xaa Xaa Xaa Xaa Xaa Xaa 
        35                  40                  45              


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
    50                  55                  60                  


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
65                  70                  75                  80  


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
                85                  90                  95      


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
            100                 105                 110         


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        115                 120                 125             


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
    130                 135                 140                 


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
145                 150                 155                 160 


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
                165                 170                 175     


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
            180                 185                 190         


<210>  9
<211>  192
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Oleosin Variant


<220>
<221>  MISC_FEATURE
<222>  (1)..(42)
<223>  Xaa at positions 1 to 42 may be any naturally-occurring or 
       artificial amino acid and up to 42 of them may be absent

<220>
<221>  MISC_FEATURE
<222>  (130)..(192)
<223>  Xaa at positions 130 to 192 may be any naturally-occurring or 
       artificial amino acid and up to 63 of them may be absent

<400>  9

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10                  15      


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
            20                  25                  30          


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ile Met Val Ile Met Ala 
        35                  40                  45              


Leu Leu Pro Ile Thr Gly Ile Leu Phe Gly Leu Ala Gly Ile Thr Leu 
    50                  55                  60                  


Val Gly Thr Val Ile Gly Leu Ala Leu Ala Thr Pro Leu Phe Val Ile 
65                  70                  75                  80  


Phe Ser Pro Val Ile Val Pro Ala Met Ile Ala Ile Gly Leu Ala Val 
                85                  90                  95      


Thr Gly Phe Leu Thr Ser Gly Thr Phe Gly Leu Thr Gly Leu Ser Ser 
            100                 105                 110         


Leu Ser Tyr Leu Phe Asn Met Val Arg Arg Ser Thr Met Ser Val Pro 
        115                 120                 125             


Val Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
    130                 135                 140                 


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
145                 150                 155                 160 


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
                165                 170                 175     


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
            180                 185                 190         


<210>  10
<211>  134
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Oleosin Variant

<400>  10

Met Ala Thr Thr Thr Tyr Asp Arg His His Val Thr Thr Thr Gln Pro 
1               5                   10                  15      


Gln Tyr Arg His Asp Gln His Thr Gly Asp Arg Leu Thr His Pro Gln 
            20                  25                  30          


Arg Gln Gln Gln Gly Pro Ser Thr Gly Lys Leu Ala Leu Ala Thr Pro 
        35                  40                  45              


Leu Phe Val Ile Phe Ser Pro Val Ile Val Pro Ala Met Ile Ala Ile 
    50                  55                  60                  


Gly Leu Ala Val Thr Gly Phe Gln Arg Asp Tyr Val Lys Gly Lys Leu 
65                  70                  75                  80  


Gln Asp Val Gly Glu Tyr Thr Gly Gln Lys Thr Lys Asp Leu Gly Gln 
                85                  90                  95      


Lys Ile Gln His Thr Ala His Glu Met Gly Asp Gln Gly Gln Gly Gln 
            100                 105                 110         


Gly Gln Gly Gly Gly Lys Glu Gly Arg Lys Glu Gly Gly Lys Leu Glu 
        115                 120                 125             


His His His His His His 
    130                 


<210>  11
<211>  142
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Oleosin Variant

<400>  11

Gly Ser Glu Ala Thr Thr Thr Asn Asp Gln His His Val Thr Thr Thr 
1               5                   10                  15      


Gln Pro Gln Asp Gln His Asp Gln His Thr Gly Asp Gln Leu Thr His 
            20                  25                  30          


Pro Gln Asp Gln Gln Gln Gly Pro Ser Thr Gly Glu Leu Ala Leu Gly 
        35                  40                  45              


Ala Thr Pro Leu Phe Gly Val Ile Gly Phe Ser Pro Val Ile Val Pro 
    50                  55                  60                  


Ala Met Gly Ile Ala Ile Gly Leu Ala Gly Val Thr Gly Phe Gln Trp 
65                  70                  75                  80  


Gln Asp Asn Val Asn Gly Glu Leu Gln Asp Val Gly Glu Gln Thr Gly 
                85                  90                  95      


Gln Asn Thr Asn Asp Leu Gly Gln Gln Ile Gln His Thr Ala His Glu 
            100                 105                 110         


Met Gly Asp Gln Gly Gln Gly Gln Gly Gln Gly Gly Gly Asn Glu Gly 
        115                 120                 125             


Gln Asn Glu Gly Gly Asn His His His His His His Asp Asp 
    130                 135                 140         


<210>  12
<211>  140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Oleosin Variant

<400>  12

Gly Ser Ala Thr Thr Thr Lys Asn Arg His His Val Thr Thr Thr Gln 
1               5                   10                  15      


Pro Gln Lys Arg His Asn Gln His Thr Gly Asn Arg Leu Thr His Pro 
            20                  25                  30          


Gln Arg Gln Gln Gln Gly Pro Ser Thr Gly Lys Leu Ala Leu Gly Ala 
        35                  40                  45              


Thr Pro Leu Phe Gly Val Ile Gly Phe Ser Pro Val Ile Val Pro Ala 
    50                  55                  60                  


Met Gly Ile Ala Ile Gly Leu Ala Gly Val Thr Gly Phe Gln Trp Asn 
65                  70                  75                  80  


Lys Val Lys Gly Lys Leu Gln Asn Val Gly Gln Lys Thr Gly Gln Lys 
                85                  90                  95      


Thr Lys Asn Leu Gly Gln Lys Ile Gln His Thr Ala His Gln Met Gly 
            100                 105                 110         


Asn Gln Gly Gln Gly Gln Gly Gln Gly Gly Gly Lys Gln Gly Arg Lys 
        115                 120                 125             


Gln Gly Gly Lys Leu Glu His His His His His His 
    130                 135                 140 


<210>  13
<211>  138
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Oleosin Variant


<220>
<221>  MISC_FEATURE
<222>  (133)..(138)
<223>  Xaa at positions 133 to 138 may be any naturally-occurring or 
       artificial amino acid and up to 6 of them may be absent

<400>  13

Gly Ser Thr Thr Thr Tyr Asp Arg His His Val Thr Thr Thr Gln Pro 
1               5                   10                  15      


Gln Tyr Arg His Asp Gln His Thr Gly Asp Arg Leu Thr His Pro Gln 
            20                  25                  30          


Arg Gln Gln Gln Gly Pro Ser Thr Gly Lys Leu Ala Leu Ala Thr Pro 
        35                  40                  45              


Leu Phe Val Ile Phe Ser Pro Val Ile Val Pro Ala Met Ile Ala Ile 
    50                  55                  60                  


Gly Leu Ala Val Thr Gly Phe Gln Arg Asp Tyr Val Lys Gly Lys Leu 
65                  70                  75                  80  


Gln Asp Val Gly Glu Tyr Thr Gly Gln Lys Thr Lys Asp Leu Gly Gln 
                85                  90                  95      


Lys Ile Gln His Thr Ala His Glu Met Gly Asp Gln Gly Gln Gly Gln 
            100                 105                 110         


Gly Gln Gly Gly Gly Lys Glu Gly Arg Lys Glu Gly Gly Lys His His 
        115                 120                 125             


His His His His Xaa Xaa Xaa Xaa Xaa Xaa 
    130                 135             


<210>  14
<211>  60
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Her-2 Affibody

<400>  14

Val Asp Asn Lys Phe Asn Lys Glu Met Arg Asn Ala Tyr Trp Glu Ile 
1               5                   10                  15      


Ala Leu Leu Pro Asn Leu Asn Asn Gln Gln Lys Arg Ala Phe Ile Arg 
            20                  25                  30          


Ser Leu Tyr Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Leu Glu 
    50                  55                  60  


<210>  15
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  15
aaggagatag gatccaccac aacctacgac c                                      31


<210>  16
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  16
gcaccgagag cgagcttgcc ggttgagg                                          28


<210>  17
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  17
cctcaaccgg caagctcgct ctcggtgc                                          28


<210>  18
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  18
ccttcacata atccctctga aacccggtaa cacc                                   34


<210>  19
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  19
ggtgttaccg ggtttcagag ggattatgtg aagg                                   34


<210>  20
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  20
tatatgaatc tcgagtttcc ccccttcttt tcg                                    33


<210>  21
<211>  105
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  21
ctcgctctcg gtgcgactcc gctgtttggt gttataggtt tcagccctgt tattgttcca       60

gcgatgggta tagcgattgg gcttgcgggt gttaccgggt ttcag                      105


<210>  22
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  22
atcggtatac atatggtgag caagggcgag g                                      31


<210>  23
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  23
atctaaaatg gatcccttgt acagctcg                                          28


