                         SEQUENCE LISTING

<110>  Zack, Donald
       Ranganathan, Vinod
 
<120>  COMPOSITIONS AND METHODS FOR THE EXPRESSION OF CRISPR GUIDE RNAS 
       USING THE H1 PROMOTER

<130>  111232-00401.P12924-02

<160>  59    

<170>  PatentIn version 3.5

<210>  1
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  1
cttcgatgtc gactcgagtc aaaaagcacc gactcggtgc cac                         43


<210>  2
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  2
aaaatatgga acgcttcacg aatttg                                            26


<210>  3
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  3
gttttagagc tagaaatagc aagttaa                                           27


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  4
aagcaccgac tcggtgccac                                                   20


<210>  5
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  5
ctcgcttcgg cagcacatat act                                               23


<210>  6
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR primer

<400>  6
acgcttcacg aatttgcgtg tc                                                22


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting sequence

<400>  7
gcactgcacg ccgtaggtca                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting sequence

<400>  8
agcactgcac gccgtaggtc                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting sequence

<400>  9
gctgaagcac tgcacgccgt                                                   20


<210>  10
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting sequence

<400>  10
gggcgcacca tcttcttca                                                    19


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting sequence

<400>  11
accatcttct tcaaggacga                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting sequence

<400>  12
gccgtcgtcc ttgaagaaga                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting sequence

<400>  13
ggtgaaccgc atcgagctga                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting sequence

<400>  14
gtgaaccgca tcgagctgaa                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting sequence

<400>  15
gcacggggcc gtcgccgatg                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting sequence

<400>  16
agcacggggc cgtcgccgat                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting sequence

<400>  17
ggtgctcagg tagtggttgt                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting sequence

<400>  18
accgccgccg ggatcactct                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting sequence

<400>  19
gtccatgccg agagtgatcc                                                   20


<210>  20
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting sequence

<400>  20
gggtgggggg agtttgctcc tgg                                               23


<210>  21
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting sequence

<400>  21
ggatggaggg agtttgctcc tgg                                               23


<210>  22
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting sequence

<400>  22
gggagggtgg agtttgctcc tgg                                               23


<210>  23
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting sequence

<400>  23
cgggggaggg agtttgctcc tgg                                               23


<210>  24
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting sequence

<400>  24
ggggagggga agtttgctcc tgg                                               23


<210>  25
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting sequence

<400>  25
ggtgagtgag tgtgtgcgtg tgg                                               23


<210>  26
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting sequence

<400>  26
ggtgagtgag tgtgtgtgtg agg                                               23


<210>  27
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting sequence

<400>  27
agtgagtgag tgtgtgtgtg agg                                               23


<210>  28
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting sequence

<400>  28
gctgagtgag tgtatgcgtg tgg                                               23


<210>  29
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting sequence

<400>  29
tgtgggtgag tgtgtgcgtg agg                                               23


<210>  30
<211>  96
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA targeting sequence


<220>
<221>  misc_feature
<222>  (2)..(20)
<223>  n is a, c, g, or u

<400>  30
annnnnnnnn nnnnnnnnnn guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugc                                 96


<210>  31
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  junction region of AAVS1 locus

<400>  31
gcctctggcc cactgtttcc ccttcccagg caggtcctgc tttctctgac ctgcattctc       60

tcccctgggc ctgt                                                         74


<210>  32
<211>  63
<212>  DNA
<213>  Homo sapiens

<400>  32
cagcctggaa gaccacatac aggaaacgta gccattcccc aggtgacctc tgtcgaatca       60

aag                                                                     63


<210>  33
<211>  74
<212>  DNA
<213>  Homo sapiens

<400>  33
acccagcctg gaagaccaca tacaggaaac gtagccattc cccaggtgac ctctgtcgaa       60

tcaaagcccc tacc                                                         74


<210>  34
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  delta 12 mutation of AN19NGG region of MERTK gene

<400>  34
acccagcctg gaagaccaca tacaggaaac gtagtgacct ctgtcgaatc aaagccccta       60

cc                                                                      62


<210>  35
<211>  73
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  delta 1 mutation of AN19NGG region of MERTK gene

<400>  35
acccagcctg gaagaccaca tacaggaaac gtagccattc ccaggtgacc tctgtcgaat       60

caaagcccct acc                                                          73


<210>  36
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  delta 2, +2 mutation of AN19NGG region of MERTK gene

<400>  36
acccagcctg gaagaccaca tacaggaaac gtagccattc gacaggtgac ctctgtcgaa       60

tcaaagcccc tacc                                                         74


<210>  37
<211>  68
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  delta 6 mutation of AN19NGG region of MERTK gene

<400>  37
acccagcctg gaagaccaca tacaggaaac gtagcccagg tgacctctgt cgaatcaaag       60

cccctacc                                                                68


<210>  38
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  delta 7 mutation of AN19NGG region of MERTK gene

<400>  38
acccagcctg gaagaccaca tacaggaaac gtagccaggt gacctctgtc gaatcaaagc       60

ccctacc                                                                 67


<210>  39
<211>  60
<212>  DNA
<213>  Homo sapiens

<400>  39
agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc gtgctcgctt       60


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U6 promoter and gRNA sequence


<220>
<221>  misc_feature
<222>  (12)..(20)
<223>  n is a, c, g, or t

<400>  40
acgaaacacc gnnnnnnnnn                                                   20


<210>  41
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U6 promoter and gRNA sequence


<220>
<221>  misc_feature
<222>  (12)..(15)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (17)..(20)
<223>  n is a, c, g, or t

<400>  41
acgaaacacc annnngnnnn                                                   20


<210>  42
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U6 promoter and gRNA sequence


<220>
<221>  misc_feature
<222>  (12)..(13)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (15)..(20)
<223>  n is a, c, g, or t

<400>  42
acgaaacacc cnngnnnnnn                                                   20


<210>  43
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  U6 promoter and gRNA sequence


<220>
<221>  misc_feature
<222>  (12)..(14)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (16)..(20)
<223>  n is a, c, g, or t

<400>  43
acgaaacacc tnnngnnnnn                                                   20


<210>  44
<211>  59
<212>  DNA
<213>  Homo sapiens

<400>  44
tctttggatt tgggaatctt ataagttctt atgagaccac tctttcccat aggggcgga        59


<210>  45
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H1 promoter and gRNA sequence


<220>
<221>  misc_feature
<222>  (11)..(19)
<223>  n is a, c, g, or t

<400>  45
actctttccg nnnnnnnnn                                                    19


<210>  46
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H1 promoter and gRNA sequence


<220>
<221>  misc_feature
<222>  (12)..(20)
<223>  n is a, c, g, or t

<400>  46
actctttccc annnnnnnnn                                                   20


<210>  47
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H1 promoter and gRNA sequence


<220>
<221>  misc_feature
<222>  (12)..(20)
<223>  n is a, c, g, or t

<400>  47
actctttccc cnnnnnnnnn                                                   20


<210>  48
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H1 promoter and gRNA sequence


<220>
<221>  misc_feature
<222>  (12)..(20)
<223>  n is a, c, g, or t

<400>  48
actctttccc tnnnnnnnnn                                                   20


<210>  49
<211>  143
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ribozyme


<220>
<221>  misc_feature
<222>  (1)..(143)
<223>  n is a, u, c, or g

<400>  49
nnnnnnnnnn cugaugaguc cgugaggacg aaacgaggaa acucgucnnn nnnnnnnnnn       60

nnnnnnnguu uuagagcuag aaauagcaag uuaaaauaag gcuaguccgu uaucaacuug      120

aaaaaguggc accgagucgg ugc                                              143


<210>  50
<211>  96
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ribozyme


<220>
<221>  misc_feature
<222>  (1)..(96)
<223>  n is a, u, c, or g

<400>  50
nnnnnnnnnn nnnnnnnnnn guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugc                                 96


<210>  51
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target site for hammerhead ribozyme

<400>  51
ctcacctcta cgccagagcg cgg                                               23


<210>  52
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  target site for hammerhead ribozyme

<400>  52
aggaaacgta gccattcccc agg                                               23


<210>  53
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hammerhead sequence

<400>  53
gtacgtttcc tctgatgagt cccaaatagg acgaaacgcg cttcggtgcg tc               52


<210>  54
<211>  230
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  H1 sequence

<400>  54
ggaattcgaa cgctgacgtc atcaacccgc tccaaggaat cgcgggccca gtgtcactag       60

gcgggaacac ccagcgcgcg tgcgccctgg caggaagatg gctgtgaggg acaggggagt      120

ggcgccctgc aatatttgca tgtcgctatg tgttctggga aatcaccata aacgtgaaat      180

gtctttggat ttgggaatct tataagttct gtatgagacc actttttccc                 230


<210>  55
<211>  82
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  ribozyme


<220>
<221>  misc_feature
<222>  (1)..(82)
<223>  n is a, u, c, or g

<400>  55
nnnnnnnnnn cugaugagau accagccgaa aggcccuugg cagugaaacg aggaaacucg       60

ucnnnnnnnn nnnnnnnnnn nn                                                82


<210>  56
<211>  27
<212>  DNA
<213>  Mus musculus

<400>  56
agccccttcg agcagccgca gtactac                                           27


<210>  57
<211>  27
<212>  DNA
<213>  Mus musculus

<400>  57
agccacttcg agcagccgca gtactac                                           27


<210>  58
<211>  27
<212>  DNA
<213>  Mus musculus

<400>  58
agccctttcg agcagccgca gtactac                                           27


<210>  59
<211>  9
<212>  PRT
<213>  Mus musculus

<400>  59

Ser Pro Phe Glu Gln Pro Gln Tyr Tyr 
1               5                   


