                         SEQUENCE LISTING

<110>  Goetz, Eva M.
       Garraway, Levi A.
 
<120>  ERK1 AND ERK2 MUTATIONS THAT CONFER RESISTANCE TO MAPK PATHWAY 
       INHIBITORS

<130>  14293-440

<150>  US 62/012,175
<151>  2014-06-13

<160>  116   

<170>  PatentIn version 3.5

<210>  1
<211>  1140
<212>  DNA
<213>  Homo sapiens

<400>  1
atggcggcgg cggcggctca ggggggcggg ggcggggagc cccgtagaac cgagggggtc       60

ggcccggggg tcccggggga ggtggagatg gtgaaggggc agccgttcga cgtgggcccg      120

cgctacacgc agttgcagta catcggcgag ggcgcgtacg gcatggtcag ctcggcctat      180

gaccacgtgc gcaagactcg cgtggccatc aagaagatca gccccttcga acatcagacc      240

tactgccagc gcacgctccg ggagatccag atcctgctgc gcttccgcca tgagaatgtc      300

atcggcatcc gagacattct gcgggcgtcc accctggaag ccatgagaga tgtctacatt      360

gtgcaggacc tgatggagac tgacctgtac aagttgctga aaagccagca gctgagcaat      420

gaccatatct gctacttcct ctaccagatc ctgcggggcc tcaagtacat ccactccgcc      480

aacgtgctcc accgagatct aaagccctcc aacctgctca tcaacaccac ctgcgacctt      540

aagatttgtg atttcggcct ggcccggatt gccgatcctg agcatgacca caccggcttc      600

ctgacggagt atgtggctac gcgctggtac cgggccccag agatcatgct gaactccaag      660

ggctatacca agtccatcga catctggtct gtgggctgca ttctggctga gatgctctct      720

aaccggccca tcttccctgg caagcactac ctggatcagc tcaaccacat tctgggcatc      780

ctgggctccc catcccagga ggacctgaat tgtatcatca acatgaaggc ccgaaactac      840

ctacagtctc tgccctccaa gaccaaggtg gcttgggcca agcttttccc caagtcagac      900

tccaaagccc ttgacctgct ggaccggatg ttaaccttta accccaataa acggatcaca      960

gtggaggaag cgctggctca cccctacctg gagcagtact atgacccgac ggatgagcca     1020

gtggccgagg agcccttcac cttcgccatg gagctggatg acctacctaa ggagcggctg     1080

aaggagctca tcttccagga gacagcacgc ttccagcccg gagtgctgga ggccccctag     1140


<210>  2
<211>  1083
<212>  DNA
<213>  Homo sapiens

<400>  2
atggcggcgg cggcggcggc gggcgcgggc ccggagatgg tccgcgggca ggtgttcgac       60

gtggggccgc gctacaccaa cctctcgtac atcggcgagg gcgcctacgg catggtgtgc      120

tctgcttatg ataatgtcaa caaagttcga gtagctatca agaaaatcag cccctttgag      180

caccagacct actgccagag aaccctgagg gagataaaaa tcttactgcg cttcagacat      240

gagaacatca ttggaatcaa tgacattatt cgagcaccaa ccatcgagca aatgaaagat      300

gtatatatag tacaggacct catggaaaca gatctttaca agctcttgaa gacacaacac      360

ctcagcaatg accatatctg ctattttctc taccagatcc tcagagggtt aaaatatatc      420

cattcagcta acgttctgca ccgtgacctc aagccttcca acctgctgct caacaccacc      480

tgtgatctca agatctgtga ctttggcctg gcccgtgttg cagatccaga ccatgatcac      540

acagggttcc tgacagaata tgtggccaca cgttggtaca gggctccaga aattatgttg      600

aattccaagg gctacaccaa gtccattgat atttggtctg taggctgcat tctggcagaa      660

atgctttcta acaggcccat ctttccaggg aagcattatc ttgaccagct gaaccacatt      720

ttgggtattc ttggatcccc atcacaagaa gacctgaatt gtataataaa tttaaaagct      780

aggaactatt tgctttctct tccacacaaa aataaggtgc catggaacag gctgttccca      840

aatgctgact ccaaagctct ggacttattg gacaaaatgt tgacattcaa cccacacaag      900

aggattgaag tagaacaggc tctggcccac ccatatctgg agcagtatta cgacccgagt      960

gacgagccca tcgccgaagc accattcaag ttcgacatgg aattggatga cttgcctaag     1020

gaaaagctca aagaactaat ttttgaagag actgctagat tccagccagg atacagatct     1080

taa                                                                   1083


<210>  3
<211>  379
<212>  PRT
<213>  Homo sapiens

<400>  3

Met Ala Ala Ala Ala Ala Gln Gly Gly Gly Gly Gly Glu Pro Arg Arg 
1               5                   10                  15      


Thr Glu Gly Val Gly Pro Gly Val Pro Gly Glu Val Glu Met Val Lys 
            20                  25                  30          


Gly Gln Pro Phe Asp Val Gly Pro Arg Tyr Thr Gln Leu Gln Tyr Ile 
        35                  40                  45              


Gly Glu Gly Ala Tyr Gly Met Val Ser Ser Ala Tyr Asp His Val Arg 
    50                  55                  60                  


Lys Thr Arg Val Ala Ile Lys Lys Ile Ser Pro Phe Glu His Gln Thr 
65                  70                  75                  80  


Tyr Cys Gln Arg Thr Leu Arg Glu Ile Gln Ile Leu Leu Arg Phe Arg 
                85                  90                  95      


His Glu Asn Val Ile Gly Ile Arg Asp Ile Leu Arg Ala Ser Thr Leu 
            100                 105                 110         


Glu Ala Met Arg Asp Val Tyr Ile Val Gln Asp Leu Met Glu Thr Asp 
        115                 120                 125             


Leu Tyr Lys Leu Leu Lys Ser Gln Gln Leu Ser Asn Asp His Ile Cys 
    130                 135                 140                 


Tyr Phe Leu Tyr Gln Ile Leu Arg Gly Leu Lys Tyr Ile His Ser Ala 
145                 150                 155                 160 


Asn Val Leu His Arg Asp Leu Lys Pro Ser Asn Leu Leu Ile Asn Thr 
                165                 170                 175     


Thr Cys Asp Leu Lys Ile Cys Asp Phe Gly Leu Ala Arg Ile Ala Asp 
            180                 185                 190         


Pro Glu His Asp His Thr Gly Phe Leu Thr Glu Tyr Val Ala Thr Arg 
        195                 200                 205             


Trp Tyr Arg Ala Pro Glu Ile Met Leu Asn Ser Lys Gly Tyr Thr Lys 
    210                 215                 220                 


Ser Ile Asp Ile Trp Ser Val Gly Cys Ile Leu Ala Glu Met Leu Ser 
225                 230                 235                 240 


Asn Arg Pro Ile Phe Pro Gly Lys His Tyr Leu Asp Gln Leu Asn His 
                245                 250                 255     


Ile Leu Gly Ile Leu Gly Ser Pro Ser Gln Glu Asp Leu Asn Cys Ile 
            260                 265                 270         


Ile Asn Met Lys Ala Arg Asn Tyr Leu Gln Ser Leu Pro Ser Lys Thr 
        275                 280                 285             


Lys Val Ala Trp Ala Lys Leu Phe Pro Lys Ser Asp Ser Lys Ala Leu 
    290                 295                 300                 


Asp Leu Leu Asp Arg Met Leu Thr Phe Asn Pro Asn Lys Arg Ile Thr 
305                 310                 315                 320 


Val Glu Glu Ala Leu Ala His Pro Tyr Leu Glu Gln Tyr Tyr Asp Pro 
                325                 330                 335     


Thr Asp Glu Pro Val Ala Glu Glu Pro Phe Thr Phe Ala Met Glu Leu 
            340                 345                 350         


Asp Asp Leu Pro Lys Glu Arg Leu Lys Glu Leu Ile Phe Gln Glu Thr 
        355                 360                 365             


Ala Arg Phe Gln Pro Gly Val Leu Glu Ala Pro 
    370                 375                 


<210>  4
<211>  360
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Ala Ala Ala Ala Ala Ala Gly Ala Gly Pro Glu Met Val Arg Gly 
1               5                   10                  15      


Gln Val Phe Asp Val Gly Pro Arg Tyr Thr Asn Leu Ser Tyr Ile Gly 
            20                  25                  30          


Glu Gly Ala Tyr Gly Met Val Cys Ser Ala Tyr Asp Asn Val Asn Lys 
        35                  40                  45              


Val Arg Val Ala Ile Lys Lys Ile Ser Pro Phe Glu His Gln Thr Tyr 
    50                  55                  60                  


Cys Gln Arg Thr Leu Arg Glu Ile Lys Ile Leu Leu Arg Phe Arg His 
65                  70                  75                  80  


Glu Asn Ile Ile Gly Ile Asn Asp Ile Ile Arg Ala Pro Thr Ile Glu 
                85                  90                  95      


Gln Met Lys Asp Val Tyr Ile Val Gln Asp Leu Met Glu Thr Asp Leu 
            100                 105                 110         


Tyr Lys Leu Leu Lys Thr Gln His Leu Ser Asn Asp His Ile Cys Tyr 
        115                 120                 125             


Phe Leu Tyr Gln Ile Leu Arg Gly Leu Lys Tyr Ile His Ser Ala Asn 
    130                 135                 140                 


Val Leu His Arg Asp Leu Lys Pro Ser Asn Leu Leu Leu Asn Thr Thr 
145                 150                 155                 160 


Cys Asp Leu Lys Ile Cys Asp Phe Gly Leu Ala Arg Val Ala Asp Pro 
                165                 170                 175     


Asp His Asp His Thr Gly Phe Leu Thr Glu Tyr Val Ala Thr Arg Trp 
            180                 185                 190         


Tyr Arg Ala Pro Glu Ile Met Leu Asn Ser Lys Gly Tyr Thr Lys Ser 
        195                 200                 205             


Ile Asp Ile Trp Ser Val Gly Cys Ile Leu Ala Glu Met Leu Ser Asn 
    210                 215                 220                 


Arg Pro Ile Phe Pro Gly Lys His Tyr Leu Asp Gln Leu Asn His Ile 
225                 230                 235                 240 


Leu Gly Ile Leu Gly Ser Pro Ser Gln Glu Asp Leu Asn Cys Ile Ile 
                245                 250                 255     


Asn Leu Lys Ala Arg Asn Tyr Leu Leu Ser Leu Pro His Lys Asn Lys 
            260                 265                 270         


Val Pro Trp Asn Arg Leu Phe Pro Asn Ala Asp Ser Lys Ala Leu Asp 
        275                 280                 285             


Leu Leu Asp Lys Met Leu Thr Phe Asn Pro His Lys Arg Ile Glu Val 
    290                 295                 300                 


Glu Gln Ala Leu Ala His Pro Tyr Leu Glu Gln Tyr Tyr Asp Pro Ser 
305                 310                 315                 320 


Asp Glu Pro Ile Ala Glu Ala Pro Phe Lys Phe Asp Met Glu Leu Asp 
                325                 330                 335     


Asp Leu Pro Lys Glu Lys Leu Lys Glu Leu Ile Phe Glu Glu Thr Ala 
            340                 345                 350         


Arg Phe Gln Pro Gly Tyr Arg Ser 
        355                 360 


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCW57.1 FWD primer

<400>  5
tggagaattg gctagcatca                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCW57.1 REV

<400>  6
tggtggaccg gttcattact                                                   20


<210>  7
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' pWZL (long#2)

<400>  7
cgatcctccc tttatccagc cctcactcct tctctagg                               38


<210>  8
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3'pWZL -20

<400>  8
ggtggaaaat aaccggaatt ggtcgagaca agtttg                                 36


<210>  9
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK1 'open'

<400>  9
gtgctggagg cccccttgcc aactttctt                                         29


<210>  10
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK1 'open'

<400>  10
aagaaagttg gcaagggggc ctccagcac                                         29


<210>  11
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK1 G23G

<400>  11
gtcggcccgg gagtcccggg g                                                 21


<210>  12
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK1 G23G

<400>  12
ccccgggact cccgggccga c                                                 21


<210>  13
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK1 V28G

<400>  13
gtcccggggg agggggagat ggtgaag                                           27


<210>  14
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK1 V28G

<400>  14
cttcaccatc tccccctccc ccgggac                                           27


<210>  15
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK1 V31G

<400>  15
gaggtggaga tggggaaggg gcagccgttc                                        30


<210>  16
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK1 V31G

<400>  16
gaacggctgc cccttcccca tctccacctc                                        30


<210>  17
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK1 I48N

<400>  17
gcagttgcag tacaacggcg agggcg                                            26


<210>  18
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK1 I48N

<400>  18
cgccctcgcc gttgtactgc aactgc                                            26


<210>  19
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK1 Y53H

<400>  19
cgagggcgcg cacggcatgg tcagc                                             25


<210>  20
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK1 Y53H

<400>  20
gctgaccatg ccgtgcgcgc cctcg                                             25


<210>  21
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK1 G54A

<400>  21
gggcgcgtac gccatggtca gctcg                                             25


<210>  22
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK1 G54A

<400>  22
cgagctgacc atggcgtacg cgccc                                             25


<210>  23
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK1 Y60C

<400>  23
gctcggcctg tgaccacgtg cgcaag                                            26


<210>  24
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK1 Y60C

<400>  24
cttgcgcacg tggtcacagg ccgagc                                            26


<210>  25
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK1 V63G

<400>  25
ctatgaccac gggcgcaaga ctcgcgtg                                          28


<210>  26
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK1 V63G

<400>  26
cacgcgagtc ttgcgcccgt ggtcatag                                          28


<210>  27
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK1 K71R

<400>  27
cgcgtggcca tcaggaagat cagcccc                                           27


<210>  28
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK1 K71R

<400>  28
ggggctgatc ttcctgatgg ccacgcg                                           27


<210>  29
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK1 S74G

<400>  29
catcaagaag atcggcccct tcgaacatca gacc                                   34


<210>  30
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK1 S74G

<400>  30
ggtctgatgt tcgaaggggc cgatcttctt gatg                                   34


<210>  31
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK1 P75L

<400>  31
ccatcaagaa gatcagcctc ttcgaacatc agacc                                  35


<210>  32
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK1 P75L

<400>  32
ggtctgatgt tcgaagaggc tgatcttctt gatgg                                  35


<210>  33
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK1 Y81C

<400>  33
cttcgaacat cagacctgct gccagcgca                                         29


<210>  34
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK1 Y81C

<400>  34
gtgcgctggc agcaggtctg atgttcgaag                                        30


<210>  35
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK1 C82Y

<400>  35
cgaacatcag acctactacc agcgcacgct cc                                     32


<210>  36
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK1 C82Y

<400>  36
ggagcgtgcg ctggtagtag gtctgatgtt cg                                     32


<210>  37
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK1 R84H

<400>  37
cagacctact gccagcacac gctccgg                                           27


<210>  38
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK1 R84H

<400>  38
ccggagcgtg tgctggcagt aggtctg                                           27


<210>  39
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK1 Q90R

<400>  39
cacgctccgg gagatccgga tcctgctg                                          28


<210>  40
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK1 Q90R

<400>  40
cagcaggatc cggatctccc ggagcgtg                                          28


<210>  41
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK1 Y148H

<400>  41
ccatatctgc tacttcctcc accagatcct gcg                                    33


<210>  42
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK1 Y148H

<400>  42
cgcaggatct ggtggaggaa gtagcagata tgg                                    33


<210>  43
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK1 T176P

<400>  43
cctgctcatc aaccccacct gcgaccttaa g                                      31


<210>  44
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK1 T176P

<400>  44
cttaaggtcg caggtggggt tgatgagcag g                                      31


<210>  45
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK1 T177P

<400>  45
cctgctcatc aacaccccct gcgaccttaa g                                      31


<210>  46
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK1 T177P

<400>  46
cttaaggtcg cagggggtgt tgatgagcag g                                      31


<210>  47
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK1 G186D

<400>  47
gatttgtgat ttcgacctgg cccggattgc cg                                     32


<210>  48
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK1 G186D

<400>  48
cggcaatccg ggccaggtcg aaatcacaaa tc                                     32


<210>  49
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK1 A206V

<400>  49
gacgggagta tgtggttacg cgctggtac                                         29


<210>  50
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK1 A206V

<400>  50
gtaccagcgc gtaaccacat actcccgtc                                         29


<210>  51
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK1 M216I

<400>  51
cccagagatc attctgaact ccaagggcta tacc                                   34


<210>  52
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK1 M216I

<400>  52
ggtatagccc ttggagttca gaatgatctc tggg                                   34


<210>  53
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK1 S219P

<400>  53
cagagatcat gctgaacccc aagggctata cc                                     32


<210>  54
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK1 S219P

<400>  54
ggtatagccc ttggggttca gcatgatctc tg                                     32


<210>  55
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK1 A303V

<400>  55
gtcagactcc aaagtccttg acctgctgga c                                      31


<210>  56
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK1 A303V

<400>  56
gtccagcagg tcaaggactt tggagtctga c                                      31


<210>  57
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK1 Y329C

<400>  57
gctcacccct gcctggagca gtactatg                                          28


<210>  58
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK1 Y329C

<400>  58
catagtactg ctccaggcag gggtgagc                                          28


<210>  59
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK1 D338N

<400>  59
actatgaccc gacgaatgag ccagtggcc                                         29


<210>  60
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK1 D338N

<400>  60
ggccactggc tcattcgtcg ggtcatagt                                         29


<210>  61
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK1 E339K

<400>  61
gacccgacgg ataagccagt ggccgagg                                          28


<210>  62
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK1 E339K

<400>  62
cctcggccac tggcttatcc gtcgggtc                                          28


<210>  63
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK1 T347A

<400>  63
gagcccttcg ccttcgccat ggagctg                                           27


<210>  64
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK1 T347A

<400>  64
cagctccatg gcgaaggcga agggctc                                           27


<210>  65
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK2 'open'

<400>  65
ttccagccag gatacagatc tttgccaact ttcttg                                 36


<210>  66
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK2 'open'

<400>  66
caagaaagtt ggcaaagatc tgtatcctgg ctggaa                                 36


<210>  67
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK2 Y36N

<400>  67
cgagggcgcc aacggcatgg tgtgc                                             25


<210>  68
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK2 Y36N

<400>  68
gcacaccatg ccgttggcgc cctcg                                             25


<210>  69
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK2 Y36H

<400>  69
cgagggcgcc cacggcatgg tgtgc                                             25


<210>  70
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK2 Y36H

<400>  70
gcacaccatg ccgtgggcgc cctcg                                             25


<210>  71
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK2 G37S

<400>  71
gagggcgcct acagcatggt gtgctctg                                          28


<210>  72
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK2 G37S

<400>  72
cagagcacac catgctgtag gcgccctc                                          28


<210>  73
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK2 K54R

<400>  73
aaagttcgag tagctatcag gaaaatcagc ccctttgag                              39


<210>  74
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK2 K54R

<400>  74
ctcaaagggg ctgattttcc tgatagctac tcgaacttt                              39


<210>  75
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK2 P58T

<400>  75
gctatcaaga aaatcagcac ctttgagcac cagacc                                 36


<210>  76
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK2 P58T

<400>  76
ggtctggtgc tcaaaggtgc tgattttctt gatagc                                 36


<210>  77
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK2 P58S

<400>  77
gctatcaaga aaatcagctc ctttgagcac cagacc                                 36


<210>  78
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK2 P58S

<400>  78
ggtctggtgc tcaaaggagc tgattttctt gata                                   34


<210>  79
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK2 Y64N

<400>  79
gcaccagacc aactgccaga gaaccctg                                          28


<210>  80
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK2 Y64N

<400>  80
cagggttctc tggcagttgg tctggtgc                                          28


<210>  81
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK2 C65Y

<400>  81
gagcaccaga cctactacca gagaaccctg ag                                     32


<210>  82
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK2 C65Y

<400>  82
ctcagggttc tctggtagta ggtctggtgc tc                                     32


<210>  83
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK2 V39G

<400>  83
cgcctacggc atggggtgct ctgcttatg                                         29


<210>  84
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK2 V39G

<400>  84
cataagcaga gcaccccatg ccgtaggcg                                         29


<210>  85
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK2 P58L

<400>  85
gctatcaaga aaatcagcct ctttgagcac cagacc                                 36


<210>  86
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK2 P58L

<400>  86
ggtctggtgc tcaaagaggc tgattttctt gatagc                                 36


<210>  87
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK2 R67K

<400>  87
ccagacctac tgccagaaaa ccctgaggga g                                      31


<210>  88
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK2 R67K

<400>  88
ctccctcagg gttttctggc agtaggtctg g                                      31


<210>  89
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK2 Q105A

<400>  89
ccatcgagca aatgaaagat gtatatatag tagcggacct catgg                       45


<210>  90
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK2 Q105A

<400>  90
ccatgaggtc cgctactata tatacatctt tcatttgctc gatgg                       45


<210>  91
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK2 H120P

<400>  91
gctcttgaag acacaacccc tcagcaatga cc                                     32


<210>  92
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK2 H120P

<400>  92
ggtcattgct gaggggttgt gtcttcaaga gc                                     32


<210>  93
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK2 Y131F

<400>  93
gaccatatct gctattttct cttccagatc ctcagaggg                              39


<210>  94
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK2 Y131F

<400>  94
ccctctgagg atctggaaga gaaaatagca gatatggtc                              39


<210>  95
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK2 T159P

<400>  95
cctgctgctc aaccccacct gtgatctcaa g                                      31


<210>  96
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK2 T159P

<400>  96
cttgagatca caggtggggt tgagcagcag g                                      31


<210>  97
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK2 T160P

<400>  97
gctgctcaac accccctgtg atctcaagat                                        30


<210>  98
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK2 T160P

<400>  98
gatcttgaga tcacaggggg tgttgagcag c                                      31


<210>  99
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK2 V214A

<400>  99
ccattgatat ttggtctgca ggctgcattc tggcag                                 36


<210>  100
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK2 V214A

<400>  100
ctgccagaat gcagcctgca gaccaaatat caatg                                  35


<210>  101
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK2 D321G

<400>  101
gtattacgac ccgagtggcg agcccatcg                                         29


<210>  102
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK2 D321G

<400>  102
cgatgggctc gccactcggg tcgtaatac                                         29


<210>  103
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK2 D321N

<400>  103
gagcagtatt acgacccgag taacgagccc atc                                    33


<210>  104
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK2 D321N

<400>  104
gatgggctcg ttactcgggt cgtaatactg ctc                                    33


<210>  105
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK2 E322K

<400>  105
cgacccgagt gacaagccca tcgccgaagc                                        30


<210>  106
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK2 E322K

<400>  106
gcttcggcga tgggcttgtc actcgggtcg                                        30


<210>  107
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK2 P323S

<400>  107
cgagtgacga gtccatcgcc gaagcacc                                          28


<210>  108
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK2 P323S

<400>  108
ggtgcttcgg cgatggactc gtcactcg                                          28


<210>  109
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK2 V104V

<400>  109
gcaaatgaaa gatgtatata tagtgcagga cctcatggaa ac                          42


<210>  110
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK2 V104V

<400>  110
gtttccatga ggtcctgcac tatatataca tctttcattt gc                          42


<210>  111
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK2 A189V

<400>  111
gacagaatat gtggtcacac gttggtacag ggc                                    33


<210>  112
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK2 A189V

<400>  112
gccctgtacc aacgtgtgac cacatattct gtc                                    33


<210>  113
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK2 S202P

<400>  113
ccagaaatta tgttgaatcc caagggctac accaag                                 36


<210>  114
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK2 S202P

<400>  114
cttggtgtag cccttgggat tcaacataat ttctgg                                 36


<210>  115
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ERK2 Y205N

<400>  115
gttgaattcc aagggcaaca ccaagtccat tgatatttg                              39


<210>  116
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GC - ERK2 Y205N

<400>  116
caaatatcaa tggacttggt gttgcccttg gaattcaac                              39


