                         SEQUENCE LISTING

<110>  SES
       SEsVanderhave
 
<120>  USE OF SELECTABLE MARKER GENE IN SUGAR BEET PROTOPLASTS 
       TRANSFORMATION METHOD AND SYSTEM

<130>  SESS0012

<150>  PCT/EP2014/062288
<151>  2014-06-12

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  1998
<212>  DNA
<213>  Beta vulgaris

<400>  1
atggcggcta ccttcacaaa cccaacattt tccccttcct caactccatt aaccaaaacc       60

ctaaaatccc aatcttccat ctcttcaacc ctcccctttt ccacccctcc caaaacccca      120

actccactct ttcaccgtcc cctccaaatc tcatcctccc aatcccacaa atcatccgcc      180

attaaaacac aaactcaagc accttcttct ccagctattg aagattcatc tttcgtttct      240

cgatttggcc ctgatgaacc cagaaaaggg tccgatgtcc tcgttgaagc tcttgagcgt      300

gaaggtgtta ccaatgtgtt tgcttaccct ggtggtgcat ctatggaaat ccaccaagct      360

ctcacacgct ctaaaaccat ccgcaatgtc ctccctcgcc atgaacaagg cggggttttc      420

gccgccgagg gatatgctag agctactgga aaggttggtg tctgcattgc gacttctggt      480

cctggtgcta ccaacctcgt atcaggtctt gctgacgctc tccttgattc tgtccctctt      540

gttgccatca ctggccaagt tccacgccgt atgattggca ctgatgcttt tcaggagact      600

ccaattgttg aggtgacaag gtctattact aagcataatt atttagtttt ggatgtagag      660

gatattccta gaattgttaa ggaagccttt tttttagcta attctggtag gcctggacct      720

gttttgattg atcttcctaa agatattcag cagcaattgg ttgttcctga ttgggatagg      780

ccttttaagt tgggtgggta tatgtctagg ctgccaaagt ccaagttttc gacgaatgag      840

gttggacttc ttgagcagat tgtgaggttg atgagtgagt cgaagaagcc tgtcttgtat      900

gtgggaggtg ggtgtttgaa ttctagtgag gagttgagga gatttgttga gttgacaggg      960

attccggtgg ctagtacttt gatggggttg gggtcttacc cttgtaatga tgaactgtct     1020

cttcatatgt tggggatgca cgggactgtt tatgccaatt atgcggtgga taaggcggat     1080

ttgttgcttg ctttcggggt taggtttgat gatcgtgtga ccgggaagct cgaggcgttt     1140

gctagccgtg ctaagattgt gcatattgat attgactctg ctgagattgg gaagaacaag     1200

cagccccatg tgtccatttg tgctgatgtt aaattggcat tgcggggtat gaataagatt     1260

ctggagtcta gaatagggaa gctgaatttg gatttctcca agtggagaga agaattaggt     1320

gagcagaaga aggaattccc actgagtttt aagacatttg gggatgcaat tcctccacaa     1380

tatgccattc aggtgcttga tgagttgacc aatggtaatg ctattataag tactggtgtt     1440

gggcagcacc aaatgtgggc tgcgcagcat tacaagtaca gaaaccctcg ccaatggctg     1500

acctctggtg ggttgggggc tatggggttt gggctaccag ccgccattgg agctgcagtt     1560

gctcgaccag atgcagtggt tgtcgatatt gatggggatg gcagttttat tatgaatgtt     1620

caagagttgg ctacaattag ggtggaaaat ctcccagtta agataatgct gctaaacaat     1680

caacatttag gtatggttgt ccaatgggaa gataggttct ataaagctaa ccgggcacat     1740

acataccttg gaaacccttc caaatctgct gatatcttcc ctgatatgct caaattcgct     1800

gaggcatgtg atattccttc tgcccgtgtt agcaacgtgg ctgatttgag ggccgccatt     1860

caaacaatgt tggatactcc agggccgtac ctgctcgatg tgattgtacc gcatcaagag     1920

catgtgttgc ctatgattcc aagtggtgcc ggtttcaagg ataccattac agagggtgat     1980

ggaagaacct cttattga                                                   1998


<210>  2
<211>  665
<212>  PRT
<213>  Beta Vulgaris

<400>  2

Met Ala Ala Thr Phe Thr Asn Pro Thr Phe Ser Pro Ser Ser Thr Gln 
1               5                   10                  15      


Leu Thr Lys Thr Leu Lys Ser Gln Ser Ser Ile Ser Ser Thr Leu Pro 
            20                  25                  30          


Phe Ser Thr Pro Pro Lys Thr Pro Thr Pro Leu Phe His Arg Pro Leu 
        35                  40                  45              


Gln Ile Ser Ser Ser Gln Ser His Lys Ser Ser Ala Ile Lys Thr Gln 
    50                  55                  60                  


Thr Gln Ala Pro Ser Ser Pro Ala Ile Glu Asp Ser Ser Phe Val Ser 
65                  70                  75                  80  


Arg Phe Gly Pro Asp Glu Pro Arg Lys Gly Ser Asp Val Leu Val Glu 
                85                  90                  95      


Ala Leu Glu Arg Glu Gly Val Thr Asn Val Phe Ala Tyr Pro Gly Gly 
            100                 105                 110         


Ala Ser Met Glu Ile His Gln Ala Leu Thr Arg Ser Lys Thr Ile Arg 
        115                 120                 125             


Asn Val Leu Pro Arg His Glu Gln Gly Gly Val Phe Ala Ala Glu Gly 
    130                 135                 140                 


Tyr Ala Arg Ala Thr Gly Lys Val Gly Val Cys Ile Ala Thr Ser Gly 
145                 150                 155                 160 


Pro Gly Ala Thr Asn Leu Val Ser Gly Leu Ala Asp Ala Leu Leu Asp 
                165                 170                 175     


Ser Val Pro Leu Val Ala Ile Thr Gly Gln Val Pro Arg Arg Met Ile 
            180                 185                 190         


Gly Thr Asp Ala Phe Gln Glu Thr Pro Ile Val Glu Val Thr Arg Ser 
        195                 200                 205             


Ile Thr Lys His Asn Tyr Leu Val Leu Asp Val Glu Asp Ile Pro Arg 
    210                 215                 220                 


Ile Val Lys Glu Ala Phe Phe Leu Ala Asn Ser Gly Arg Pro Gly Pro 
225                 230                 235                 240 


Val Leu Ile Asp Leu Pro Lys Asp Ile Gln Gln Gln Leu Val Val Pro 
                245                 250                 255     


Asp Trp Asp Arg Pro Phe Lys Leu Gly Gly Tyr Met Ser Arg Leu Pro 
            260                 265                 270         


Lys Ser Lys Phe Ser Thr Asn Glu Val Gly Leu Leu Glu Gln Ile Val 
        275                 280                 285             


Arg Leu Met Ser Glu Ser Lys Lys Pro Val Leu Tyr Val Gly Gly Gly 
    290                 295                 300                 


Cys Leu Asn Ser Ser Glu Glu Leu Arg Arg Phe Val Glu Leu Thr Gly 
305                 310                 315                 320 


Ile Pro Val Ala Ser Thr Leu Met Gly Leu Gly Ser Tyr Pro Cys Asn 
                325                 330                 335     


Asp Glu Leu Ser Leu His Met Leu Gly Met His Gly Thr Val Tyr Ala 
            340                 345                 350         


Asn Tyr Ala Val Asp Lys Ala Asp Leu Leu Leu Ala Phe Gly Val Arg 
        355                 360                 365             


Phe Asp Asp Arg Val Thr Gly Lys Leu Glu Ala Phe Ala Ser Arg Ala 
    370                 375                 380                 


Lys Ile Val His Ile Asp Ile Asp Ser Ala Glu Ile Gly Lys Asn Lys 
385                 390                 395                 400 


Gln Pro His Val Ser Ile Cys Ala Asp Val Lys Leu Ala Leu Arg Gly 
                405                 410                 415     


Met Asn Lys Ile Leu Glu Ser Arg Ile Gly Lys Leu Asn Leu Asp Phe 
            420                 425                 430         


Ser Arg Trp Arg Glu Glu Leu Gly Glu Gln Lys Lys Glu Phe Pro Leu 
        435                 440                 445             


Ser Phe Lys Thr Phe Gly Asp Ala Ile Pro Pro Gln Tyr Ala Ile Gln 
    450                 455                 460                 


Val Leu Asp Glu Leu Thr Asn Gly Asn Ala Ile Ile Ser Thr Gly Val 
465                 470                 475                 480 


Gly Gln His Gln Met Trp Ala Ala Gln His Tyr Lys Tyr Arg Asn Pro 
                485                 490                 495     


Arg Gln Trp Leu Thr Ser Gly Gly Leu Gly Ala Met Gly Phe Gly Leu 
            500                 505                 510         


Pro Ala Ala Ile Gly Ala Ala Val Ala Arg Pro Asp Ala Val Val Val 
        515                 520                 525             


Asp Ile Asp Gly Asp Gly Ser Phe Ile Met Asn Val Gln Glu Leu Ala 
    530                 535                 540                 


Thr Ile Arg Val Glu Asn Leu Pro Val Lys Ile Met Leu Leu Asn Asn 
545                 550                 555                 560 


Gln His Leu Gly Met Val Val Gln Trp Glu Asp Arg Phe Tyr Lys Ala 
                565                 570                 575     


Asn Arg Ala His Thr Tyr Leu Gly Asn Pro Ser Lys Ser Ala Asp Ile 
            580                 585                 590         


Phe Pro Asp Met Leu Lys Phe Ala Glu Ala Cys Asp Ile Pro Ser Ala 
        595                 600                 605             


Arg Val Ser Asn Val Ala Asp Leu Arg Ala Ala Ile Gln Thr Met Leu 
    610                 615                 620                 


Asp Thr Pro Gly Pro Tyr Leu Leu Asp Val Ile Val Pro His Gln Glu 
625                 630                 635                 640 


His Val Leu Pro Met Ile Pro Ser Gly Ala Gly Phe Lys Asp Thr Ile 
                645                 650                 655     


Thr Glu Gly Asp Gly Arg Thr Ser Tyr 
            660                 665 


<210>  3
<211>  1998
<212>  DNA
<213>  Beta Vulgaris

<400>  3
atggcggcta ccttcacaaa cccaacattt tccccttcct caactccatt aaccaaaacc       60

ctaaaatccc aatcttccat ctcttcaacc ctcccctttt ccacccctcc caaaacccca      120

actccactct ttcaccgtcc cctccaaatc tcatcctccc aatcccacaa atcatccgcc      180

attaaaacac aaactcaagc accttcttct ccagctattg aagattcatc tttcgtttct      240

cgatttggcc ctgatgaacc cagaaaaggg tccgatgtcc tcgttgaagc tcttgagcgt      300

gaaggtgtta ccaatgtgtt tgcttaccct ggtggttatt ctatggaaat ccaccaagct      360

ctcacacgct ctaaaaccat ccgcaatgtc ctccctcgcc atgaacaagg cggggttttc      420

gccgccgagg gatatgctag agctactgga aaggttggtg tctgcattgc gacttctggt      480

cctggtgcta ccaacctcgt atcaggtctt gctgacgctc tccttgattc tgtccctctt      540

gttgccatca ctggccaagt tccacgccgt atgattggca ctgatgcttt tcaggagact      600

ccaattgttg aggtgacaag gtctattact aagcataatt atttagtttt ggatgtagag      660

gatattccta gaattgttaa ggaagccttt tttttagcta attctggtag gcctggacct      720

gttttgattg atcttcctaa agatattcag cagcaattgg ttgttcctga ttgggatagg      780

ccttttaagt tgggtgggta tatgtctagg ctgccaaagt ccaagttttc gacgaatgag      840

gttggacttc ttgagcagat tgtgaggttg atgagtgagt cgaagaagcc tgtcttgtat      900

gtgggaggtg ggtgtttgaa ttctagtgag gagttgagga gatttgttga gttgacaggg      960

attccggtgg ctagtacttt gatggggttg gggtcttacc cttgtaatga tgaactgtct     1020

cttcatatgt tggggatgca cgggactgtt tatgccaatt atgcggtgga taaggcggat     1080

ttgttgcttg ctttcggggt taggtttgat gatcgtgtga ccgggaagct cgaggcgttt     1140

gctagccgtg ctaagattgt gcatattgat attgactctg ctgagattgg gaagaacaag     1200

cagccccatg tgtccatttg tgctgatgtt aaattggcat tgcggggtat gaataagatt     1260

ctggagtcta gaatagggaa gctgaatttg gatttctcca agtggagaga agaattaggt     1320

gagcagaaga aggaattccc actgagtttt aagacatttg gggatgcaat tcctccacaa     1380

tatgccattc aggtgcttga tgagttgacc aatggtaatg ctattataag tactggtgtt     1440

gggcagcacc aaatgtgggc tgcgcagcat tacaagtaca gaaaccctcg ccaatggctg     1500

acctctggtg ggttgggggc tatggggttt gggctaccag ccgccattgg agctgcagtt     1560

gctcgaccag atgcagtggt tgtcgatatt gatggggatg gcagttttat tatgaatgtt     1620

caagagttgg ctacaattag ggtggaaaat ctcccagtta agataatgct gctaaacaat     1680

caacatttag gtatggttgt ccaatgggaa gataggttct ataaagctaa ccgggcacat     1740

acataccttg gaaacccttc caaatctgct gatatcttcc ctgatatgct caaattcgct     1800

gaggcatgtg atattccttc tgcccgtgtt agcaacgtgg ctgatttgag ggccgccatt     1860

caaacaatgt tggatactcc agggccgtac ctgctcgatg tgattgtacc gcatcaagag     1920

catgtgttgc ctatgattcc aagtggtgcc ggtttcaagg ataccattac agagggtgat     1980

ggaagaacct cttattga                                                   1998


<210>  4
<211>  665
<212>  PRT
<213>  Beta Vulgaris

<400>  4

Met Ala Ala Thr Phe Thr Asn Pro Thr Phe Ser Pro Ser Ser Thr Gln 
1               5                   10                  15      


Leu Thr Lys Thr Leu Lys Ser Gln Ser Ser Ile Ser Ser Thr Leu Pro 
            20                  25                  30          


Phe Ser Thr Pro Pro Lys Thr Pro Thr Pro Leu Phe His Arg Pro Leu 
        35                  40                  45              


Gln Ile Ser Ser Ser Gln Ser His Lys Ser Ser Ala Ile Lys Thr Gln 
    50                  55                  60                  


Thr Gln Ala Pro Ser Ser Pro Ala Ile Glu Asp Ser Ser Phe Val Ser 
65                  70                  75                  80  


Arg Phe Gly Pro Asp Glu Pro Arg Lys Gly Ser Asp Val Leu Val Glu 
                85                  90                  95      


Ala Leu Glu Arg Glu Gly Val Thr Asn Val Phe Ala Tyr Pro Gly Gly 
            100                 105                 110         


Tyr Ser Met Glu Ile His Gln Ala Leu Thr Arg Ser Lys Thr Ile Arg 
        115                 120                 125             


Asn Val Leu Pro Arg His Glu Gln Gly Gly Val Phe Ala Ala Glu Gly 
    130                 135                 140                 


Tyr Ala Arg Ala Thr Gly Lys Val Gly Val Cys Ile Ala Thr Ser Gly 
145                 150                 155                 160 


Pro Gly Ala Thr Asn Leu Val Ser Gly Leu Ala Asp Ala Leu Leu Asp 
                165                 170                 175     


Ser Val Pro Leu Val Ala Ile Thr Gly Gln Val Pro Arg Arg Met Ile 
            180                 185                 190         


Gly Thr Asp Ala Phe Gln Glu Thr Pro Ile Val Glu Val Thr Arg Ser 
        195                 200                 205             


Ile Thr Lys His Asn Tyr Leu Val Leu Asp Val Glu Asp Ile Pro Arg 
    210                 215                 220                 


Ile Val Lys Glu Ala Phe Phe Leu Ala Asn Ser Gly Arg Pro Gly Pro 
225                 230                 235                 240 


Val Leu Ile Asp Leu Pro Lys Asp Ile Gln Gln Gln Leu Val Val Pro 
                245                 250                 255     


Asp Trp Asp Arg Pro Phe Lys Leu Gly Gly Tyr Met Ser Arg Leu Pro 
            260                 265                 270         


Lys Ser Lys Phe Ser Thr Asn Glu Val Gly Leu Leu Glu Gln Ile Val 
        275                 280                 285             


Arg Leu Met Ser Glu Ser Lys Lys Pro Val Leu Tyr Val Gly Gly Gly 
    290                 295                 300                 


Cys Leu Asn Ser Ser Glu Glu Leu Arg Arg Phe Val Glu Leu Thr Gly 
305                 310                 315                 320 


Ile Pro Val Ala Ser Thr Leu Met Gly Leu Gly Ser Tyr Pro Cys Asn 
                325                 330                 335     


Asp Glu Leu Ser Leu His Met Leu Gly Met His Gly Thr Val Tyr Ala 
            340                 345                 350         


Asn Tyr Ala Val Asp Lys Ala Asp Leu Leu Leu Ala Phe Gly Val Arg 
        355                 360                 365             


Phe Asp Asp Arg Val Thr Gly Lys Leu Glu Ala Phe Ala Ser Arg Ala 
    370                 375                 380                 


Lys Ile Val His Ile Asp Ile Asp Ser Ala Glu Ile Gly Lys Asn Lys 
385                 390                 395                 400 


Gln Pro His Val Ser Ile Cys Ala Asp Val Lys Leu Ala Leu Arg Gly 
                405                 410                 415     


Met Asn Lys Ile Leu Glu Ser Arg Ile Gly Lys Leu Asn Leu Asp Phe 
            420                 425                 430         


Ser Arg Trp Arg Glu Glu Leu Gly Glu Gln Lys Lys Glu Phe Pro Leu 
        435                 440                 445             


Ser Phe Lys Thr Phe Gly Asp Ala Ile Pro Pro Gln Tyr Ala Ile Gln 
    450                 455                 460                 


Val Leu Asp Glu Leu Thr Asn Gly Asn Ala Ile Ile Ser Thr Gly Val 
465                 470                 475                 480 


Gly Gln His Gln Met Trp Ala Ala Gln His Tyr Lys Tyr Arg Asn Pro 
                485                 490                 495     


Arg Gln Trp Leu Thr Ser Gly Gly Leu Gly Ala Met Gly Phe Gly Leu 
            500                 505                 510         


Pro Ala Ala Ile Gly Ala Ala Val Ala Arg Pro Asp Ala Val Val Val 
        515                 520                 525             


Asp Ile Asp Gly Asp Gly Ser Phe Ile Met Asn Val Gln Glu Leu Ala 
    530                 535                 540                 


Thr Ile Arg Val Glu Asn Leu Pro Val Lys Ile Met Leu Leu Asn Asn 
545                 550                 555                 560 


Gln His Leu Gly Met Val Val Gln Trp Glu Asp Arg Phe Tyr Lys Ala 
                565                 570                 575     


Asn Arg Ala His Thr Tyr Leu Gly Asn Pro Ser Lys Ser Ala Asp Ile 
            580                 585                 590         


Phe Pro Asp Met Leu Lys Phe Ala Glu Ala Cys Asp Ile Pro Ser Ala 
        595                 600                 605             


Arg Val Ser Asn Val Ala Asp Leu Arg Ala Ala Ile Gln Thr Met Leu 
    610                 615                 620                 


Asp Thr Pro Gly Pro Tyr Leu Leu Asp Val Ile Val Pro His Gln Glu 
625                 630                 635                 640 


His Val Leu Pro Met Ile Pro Ser Gly Ala Gly Phe Lys Asp Thr Ile 
                645                 650                 655     


Thr Glu Gly Asp Gly Arg Thr Ser Tyr 
            660                 665 


