                                        LISTAGEM DE SEQUNCIAS

<110>    OUROFINO SADE ANIMAL LTDA

<120> VETOR DE EXPRESSO T7 DE Escherichia coli, VETORES PARA A CO-EXPRESSO E CO-PURIFICAO DE POLIPEPTDEOS RECOMBINANTES EM/COM PROTENAS CARREGADORAS, USO DE VETORES DE EXPRESSO NA OBTENO DE COMPLEXOS COM MLTIPLOS ANTGENOS E IMUNOMODULADORES 
<130>    VETORES EXOSSOMO

<160>    40 

<170>    PatentIn version 3.5

<210>    SEQ ID No: 1
<211>    2822 
<212>    DNA
<213>    Sequncia artificial

<220>
<223>    Sequncia do vetor de expresso T7 pAE

<220>
<221>    primer_ligao
<222>    (825) ... (843)
<223>    ligao do primer VecMar-Rev

<220>
<221>    primer_ligao
<222>    (1818) ... (1837)
<223>    ligao do primer VecMar-For

<300>
<301>    Ramos CR, Abreu PA, Nascimento AL, Ho PL
<302>    A high-copy T7 Escherichia coli expression vector for the production of recombinant proteins with a minimal N-terminal His-tagged fusion peptide
<303>    Braz J Med Biol Res.
<304>    37
<305>    8
<306>    1103-1109
<307>    08/2004
<313>    (1)..(2822)

<400>    1

gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct       60

agaaataatt ttgtttaact ttaagaagga gatatacata tgcatcacca tcaccatcac      120

ctcgagggat ccgacctcga gatctgcagc tggtaccatg gaattcgaag cttgatccgg      180

ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag      240

cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta      300

tatccggatc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg      360

cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt      420

ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt      480

cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct      540

ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg      600

tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga      660

gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc      720

ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga      780

gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt      840

ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca      900

aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg      960

aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc     1020

cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg     1080

ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt     1140

cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta     1200

ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat     1260

gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga     1320

gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca     1380

acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact     1440

cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc     1500

acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact     1560

ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt     1620

ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt     1680

gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt     1740

atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata     1800

ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag     1860

attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat     1920

ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa     1980

aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca     2040

aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt     2100

ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg     2160

tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc     2220

ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga     2280

cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc     2340

agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc     2400

gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca     2460

ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg     2520

tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta     2580

tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct     2640

cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag     2700

tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa     2760

gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc     2820

ag                                                                    2822

 

<210>    2
<211>    28 
<212>    DNA
<213>    sequncia artificial

<220>
<223>    primer forward do gene da canamicina para PCR

<400>    2 

atctcgagtt atggacagca agcgaacc                                          28


<210>    3
<211>    27 
<212>    DNA
<213>    sequncia artificial

<220>
<223>    primer inverso do gene da canamicina para PCR

<40>    3

catctagaat ttcgaacccc agagtcc                                           27


<210>    SEQ ID No: 4 
<211>    990 
<212>    DNA
<213>    Escherichia coli

<220>
<221>    CDS
<222>    (165) ... (959)

<400>    4 

atctcgagtt atggacagca agcgaaccgg aattgccagc tggggcgccc tctggtaagg       60

ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc gccaaggatc tgatggcgca      120

ggggatcaag atctgatcaa gagacaggat gaggatcgtt tcgc atg att gaa caa       176
                                                 Met Ile Glu Gln          
                                                 1                        

gat gga ttg cac gca ggt tct ccg gcc gct tgg gtg gag agg cta ttc        224
Asp Gly Leu His Ala Gly Ser Pro Ala Ala Trp Val Glu Arg Leu Phe           
5                   10                  15                  20            

ggc tat gac tgg gca caa cag aca atc ggc tgc tct gat gcc gcc gtg        272
Gly Tyr Asp Trp Ala Gln Gln Thr Ile Gly Cys Ser Asp Ala Ala Val           
                25                  30                  35                

ttc cgg ctg tca gcg cag ggg cgc ccg gtt ctt ttt gtc aag acc gac        320
Phe Arg Leu Ser Ala Gln Gly Arg Pro Val Leu Phe Val Lys Thr Asp           
            40                  45                  50                    

ctg tcc ggt gcc ctg aat gaa ctc caa gac gag gca gcg cgg cta tcg        368
Leu Ser Gly Ala Leu Asn Glu Leu Gln Asp Glu Ala Ala Arg Leu Ser           
        55                  60                  65                        

tgg ctg gcc acg acg ggc gtt cct tgc gca gct gtg ctc gac gtt gtc        416
Trp Leu Ala Thr Thr Gly Val Pro Cys Ala Ala Val Leu Asp Val Val           
    70                  75                  80                            

act gaa gcg gga agg gac tgg ctg cta ttg ggc gaa gtg ccg ggg cag        464
Thr Glu Ala Gly Arg Asp Trp Leu Leu Leu Gly Glu Val Pro Gly Gln           
85                  90                  95                  100           

gat ctc ctg tca tct cac ctt gct cct gcc gag aaa gta tcc atc atg        512
Asp Leu Leu Ser Ser His Leu Ala Pro Ala Glu Lys Val Ser Ile Met           
                105                 110                 115               

gct gat gca atg cgg cgg ctg cat acg ctt gat ccg gct acc tgc cca        560
Ala Asp Ala Met Arg Arg Leu His Thr Leu Asp Pro Ala Thr Cys Pro           
            120                 125                 130                   

ttc gac cac caa gcg aaa cat cgc atc gag cga gca cgt act cgg atg        608
Phe Asp His Gln Ala Lys His Arg Ile Glu Arg Ala Arg Thr Arg Met           
        135                 140                 145                       

gaa gcc ggt ctt gtc gat cag gat gat ctg gac gaa gag cat cag ggg        656
Glu Ala Gly Leu Val Asp Gln Asp Asp Leu Asp Glu Glu His Gln Gly           
    150                 155                 160                           

ctc gcg cca gcc gaa ctg ttc gcc agg ctc aag gcg cgg atg ccc gac        704
Leu Ala Pro Ala Glu Leu Phe Ala Arg Leu Lys Ala Arg Met Pro Asp           
165                 170                 175                 180           

ggc gag gat ctc gtc gtg acc cat ggc gat gcc tgc ttg ccg aat atc        752
Gly Glu Asp Leu Val Val Thr His Gly Asp Ala Cys Leu Pro Asn Ile           
                185                 190                 195               

atg gtg gaa aat ggc cgc ttt tct gga ttc atc gac tgt ggc cgg ctg        800
Met Val Glu Asn Gly Arg Phe Ser Gly Phe Ile Asp Cys Gly Arg Leu           
            200                 205                 210                   

ggt gtg gcg gac cgc tat cag gac ata gcg ttg gct acc cgt gat att        848
Gly Val Ala Asp Arg Tyr Gln Asp Ile Ala Leu Ala Thr Arg Asp Ile           
        215                 220                 225                       

gct gaa gag ctt ggc ggc gaa tgg gct gac cgc ttc ctc gtg ctt tac        896
Ala Glu Glu Leu Gly Gly Glu Trp Ala Asp Arg Phe Leu Val Leu Tyr           
    230                 235                 240                           

ggt atc gcc gct ccc gat tcg cag cgc atc gcc ttc tat cgc ctt ctt        944
Gly Ile Ala Ala Pro Asp Ser Gln Arg Ile Ala Phe Tyr Arg Leu Leu           
245                 250                 255                 260           

gac gag ttc ttc tga gcgggactct ggggttcgaa atgtctagat g                 990
Asp Glu Phe Phe                                                           



<210>    SEQ ID No: 5 
<211>    264
<212>    protena
<213>    Escherichia coli

<400>    5 

Met Ile Glu Gln Asp Gly Leu His Ala Gly Ser Pro Ala Ala Trp Val 
1               5                   10                  15      


Glu Arg Leu Phe Gly Tyr Asp Trp Ala Gln Gln Thr Ile Gly Cys Ser 
            20                  25                  30          


Asp Ala Ala Val Phe Arg Leu Ser Ala Gln Gly Arg Pro Val Leu Phe 
        35                  40                  45              


Val Lys Thr Asp Leu Ser Gly Ala Leu Asn Glu Leu Gln Asp Glu Ala 
50                  55                  60                  


Ala Arg Leu Ser Trp Leu Ala Thr Thr Gly Val Pro Cys Ala Ala Val 
65                  70                  75                  80  


Leu Asp Val Val Thr Glu Ala Gly Arg Asp Trp Leu Leu Leu Gly Glu 
                85                  90                  95      


Val Pro Gly Gln Asp Leu Leu Ser Ser His Leu Ala Pro Ala Glu Lys 
100                 105                 110         


Val Ser Ile Met Ala Asp Ala Met Arg Arg Leu His Thr Leu Asp Pro 
        115                 120                 125             


Ala Thr Cys Pro Phe Asp His Gln Ala Lys His Arg Ile Glu Arg Ala 
    130                 135                 140                 


Arg Thr Arg Met Glu Ala Gly Leu Val Asp Gln Asp Asp Leu Asp Glu 
145                 150                 155                 160 


Glu His Gln Gly Leu Ala Pro Ala Glu Leu Phe Ala Arg Leu Lys Ala 
                165                 170                 175     


Arg Met Pro Asp Gly Glu Asp Leu Val Val Thr His Gly Asp Ala Cys 
            180                 185                 190         


Leu Pro Asn Ile Met Val Glu Asn Gly Arg Phe Ser Gly Phe Ile Asp 
        195                 200                 205             


Cys Gly Arg Leu Gly Val Ala Asp Arg Tyr Gln Asp Ile Ala Leu Ala 
    210                 215                 220                 


Thr Arg Asp Ile Ala Glu Glu Leu Gly Gly Glu Trp Ala Asp Arg Phe 
225                 230                 235                 240 


Leu Val Leu Tyr Gly Ile Ala Ala Pro Asp Ser Gln Arg Ile Ala Phe 
245                 250                 255     


Tyr Arg Leu Leu Asp Glu Phe Phe 
            260                 



<210>    SEQ ID No: 6 
<211>    28 
<212>    DNA
<213>    sequncia artificial

<220>
<223>    Primer forward para amplificao por PCR do vetor pAE sem o gene de AmpR 

<400>    6 

cgactagtgc attggtaact gtcagacc                                          28



<210>    SEQ ID No: 7 
<211>    29
<212>    DNA
<213>    sequncia artificial

<220>
<223> Primer inverso para amplificao por PCR do vetor pAE sem o gene de AmpR 

<400> 7 

atgtcgacgt gccacctaaa ttgtaagcg                                         29



<210>    SEQ ID No: 8 
<211>    2835
<212>    DNA
<213>    sequncia artificial

<220>
<223>    Vetor derivado do pAE com marcador KanR ao invs de AmpR

<220>
<221>     primer_ligao
<222>    (2590) ... (2609)
<223>    Ligao para o primer de PCR VecPar-Rev

<220>
<221>    primer_ligao
<222>    (2610) ... (2630)
<223>    Ligao para o primer de PCR VecPar-For

<210>    8 

gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct       60

agaaataatt ttgtttaact ttaagaagga gatatacata tgcatcacca tcaccatcac      120

ctcgagggat ccgacctcga gatctgcagc tggtaccatg gaattcgaag cttgatccgg      180

ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag      240

cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta      300

tatccggatc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg      360

cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt      420

ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt      480

cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct      540

ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg      600

tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga      660

gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc      720

ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga      780

gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt      840

ggcacgtcga gttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta      900

aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc      960

gcaggggatc aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag     1020

atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg     1080

cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc     1140

cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactccaa gacgaggcag     1200

cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca     1260

ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat     1320

ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata     1380

cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac     1440

gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc     1500

tcgcgccagc cgaactgttc gccaggctca aggcgcggat gcccgacggc gaggatctcg     1560

tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg     1620

gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta     1680

cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg     1740

gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct     1800

gagcgggact ctggggttcg aaattctagt gcattggtaa ctgtcagacc aagtttactc     1860

atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat     1920

cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc     1980

agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg     2040

ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct     2100

accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct     2160

tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct     2220

cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg     2280

gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc     2340

gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga     2400

gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg     2460

cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta     2520

tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg     2580

ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg     2640

ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat     2700

taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc     2760

agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc     2820

gattcattaa tgcag                                                      2835



<210>    SEQ ID No: 9 
<211>    27
<212>    DNA
<213>    sequncia artificial

<220>
<223>    Primer forward para amplificao por PCR para a sequncia par do plasmdeo pSC101 

<400>    9 

atctcgagtt tgtctccgac catcagg                                           27



<210>    SEQ ID No: 10 
<211>    26
<212>    DNA
<213>    sequncia artificial

<220>
<223>    Primer inverso para amplificao por PCR para a sequncia par do plasmdeo pSC101 

<400>    10 

gttctagacg ggataatccg aagtgg                                            26
 
                

<210>    SEQ ID No: 11 
<211>    287  
<212>    DNA
<213>    sequncia artificial

<220>
<223>    Sequncia par amplificada a partir do plasmdeo pSC101

<300>
<301>    Miller CA, Tucker WT, Meacock PA, Gustafsson P, Cohen SN
<302>    Nucleotide sequence of the partition locus of Escherichia coli plasmid pSC101
<303>    Gene
<304>    24
<305>    2
<306>    309-315
<307>    10/1983
<313>    (7)..(281)

<300>
<301>    Miller CA, Tucker WT, Meacock PA, Gustafsson P, Cohen SN
<302>    Nucleotide sequence of the partition locus of Escherichia coli plasmid pSC101
<303>    Genes and Development
<304>    24
<305>    2
<306>    309-315
<307>    10/1983
<313>    (7)..(281)

<400>    11 

atctcgagtt tgtctccgac catcaggcac ctgagtcgct gtctttttcg tgacattcag       60

ttcgctgcgc tcacggctct ggcagtgaat gggggtaaat ggcactacag gcgcctttta      120

tggattcatg caaggaaact acccataata caagaaaagc ccgtcacggg cttctcaggg      180

cgttttatgg cgggtctgct atgtggtgct atctgacttt ttgctgttca gcagttcctg      240

ccctctgatt ttccagtctg accacttcgg attatcccgt ctagaac                    287



<210> SEQ ID No: 12 
<211> 29
<212> DNA
<213> sequncia artificial 

<220>
<223> Primer forward para amplificao por PCR do plasmdeo pMK para insero na sequncia par

<400> 12 

atactagtac gcggcctttt tacggttcc                                         29



<210>    SEQ ID No: 13 
<211>    28 
<212>    DNA
<213>    Sequncia artificial

<220>
<223>   Primer inverso para amplificao por PCR do plasmdeo pMK para insero na sequncia par

<400>    13 

atgtcgactg ctggcgtttt tccatagg                                          28




<210>    SEQ ID No: 14 
<211>    3118 
<212>    DNA
<213>    sequncia artificial

<220>
<223>   Vetor derivado do pAE com o marcador KanR e o inserto da sequncia par

<400>    14 

gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct       60

agaaataatt ttgtttaact ttaagaagga gatatacata tgcatcacca tcaccatcac      120

ctcgagggat ccgacctcga gatctgcagc tggtaccatg gaattcgaag cttgatccgg      180

ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag      240

cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta      300

tatccggatc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg      360

cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt      420

ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt      480

cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct      540

ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg      600

tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga      660

gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc      720

ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga      780

gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt      840

ggcacgtcga gttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta      900

aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc      960

gcaggggatc aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag     1020

atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg     1080

cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc     1140

cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactccaa gacgaggcag     1200

cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca     1260

ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat     1320

ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata     1380

cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac     1440

gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc     1500

tcgcgccagc cgaactgttc gccaggctca aggcgcggat gcccgacggc gaggatctcg     1560

tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg     1620

gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta     1680

cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg     1740

gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct     1800

gagcgggact ctggggttcg aaattctagt gcattggtaa ctgtcagacc aagtttactc     1860

atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat     1920

cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc     1980

agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg     2040

ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct     2100

accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct     2160

tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct     2220

cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg     2280

gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc     2340

gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga     2400

gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg     2460

cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta     2520

tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg     2580

ggggcggagc ctatggaaaa acgccagcag tcgagtttgt ctccgaccat caggcacctg     2640

agtcgctgtc tttttcgtga cattcagttc gctgcgctca cggctctggc agtgaatggg     2700

ggtaaatggc actacaggcg ccttttatgg attcatgcaa ggaaactacc cataatacaa     2760

gaaaagcccg tcacgggctt ctcagggcgt tttatggcgg gtctgctatg tggtgctatc     2820

tgactttttg ctgttcagca gttcctgccc tctgattttc cagtctgacc acttcggatt     2880

atcccgtcta gtacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca     2940

tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag     3000

ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg     3060

aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcag       3118




<210>    SEQ ID No: 15 
<211>    42
<212>    DNA
<213>    sequncia artificial

<220>
<223>   Oligonucleotdeo forward para mutagnese do C976T no stio de restrio BglII do gene KanR

<400>    15 

gatggcgcag gggatcaaga tttgatcaag agacaggatg ag                          42




<210>    SEQ ID No: 16 
<211>    42
<212>    DNA
<213>    sequncia artificial

<220>
<223>   Oligonucleotdeo inverso para mutagnese do C976T no stio de restrio BglII do gene KanR 

<400>    16 

ctcatcctgt ctcttgatca aatcttgatc ccctgcgcca tc                          42


<210>    SEQ ID No: 17 
<211>    33 
<212>    DNA
<213>    sequncia artificial

<220>
<223>   Oligonucleotdeo forward para mutagnese do T1571C no stio de restrio NcoI do gene KanR

<400>    17 

atctcgtcgt gacccacggc gatgcctgct tgc                                    33




<210>    SEQ ID No: 18 
<211>    33 
<212>    DNA
<213>    sequncia artificial

<220>
<223>   Oligonucleotdeo inverso para mutagnese do T1571C no stio de restrio NcoI do gene KanR

<400>    18 

gcaagcaggc atcgccgtgg gtcacgacga gat                                    33

  


<210>    SEQ ID No: 19 
<211>    3118 
<212>    DNA
<213>    sequncia artificial

<220>
<223>   Vetor derivado do pMRK com a dupla mutao C976T e T1571C, que elimina os stios de resprio para BglII e NcoI, respectivamente, no gene de KanR

<220>
<221>    promotor
<222>    (20) ... (39)
<223>    promotor T7

<220>
<221>    CDS
<222>    (100) ... (240)
<223>    Fusio hexe histidina

<220>
<221>    misc_feature
<222>    (103) ... (110)
<223>    6xHis tag

<220>
<221>    mis_feature
<222>    (121) ... (173)
<223>    Stios de clonagem Mltipla

<220>
<221>    terminador
<222>    (189) ... (313)
<223>    Terminao de transcrio de T7

<220>
<221>    primer_ligao
<222>    (223) ... (242)
<223>    Primer inverso de T7

<220>
<221>    rep_origem
<222>    (384) ... (839)
<223>    Origem do fago f1

<220>
<221>    promotor
<222>    (858) ... (930)
<223>    Promotor da Neomicina Fosfotransferase (gene KanR)

<220>
<221>    CDS
<222>    (1008) ... (1802)
<223>    Gene para a Neomicina Fosfotransferase (gene KanR)

<220>
<221>    rep_origem
<222>    (1986) ... (2941)
<223>    origem do pUC

<220>
<221>    misc_feature
<222>    (2611) ... (2689)
<223>    sequncia par

<400>    19 

gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct       60

agaaataatt ttgtttaact ttaagaagga gatatacat atg cat cac cat cac         114
                                           Met His His His His            
                                           1               5              

cat cac ctc gag gga tcc gac ctc gag atc tgc agc tgg tac cat gga        162
His His Leu Glu Gly Ser Asp Leu Glu Ile Cys Ser Trp Tyr His Gly           
                10                  15                  20                

att cga agc ttg atc cgg ctg cta aca aag ccc gaa agg aag ctg agt        210
Ile Arg Ser Leu Ile Arg Leu Leu Thr Lys Pro Glu Arg Lys Leu Ser           
            25                  30                  35                    

tgg ctg ctg cca ccg ctg agc aat aac tag cataacccct tggggcctct          260
Trp Leu Leu Pro Pro Leu Ser Asn Asn                                       
        40                  45                                            

aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatc tggcgtaata      320

gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg      380

acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg      440

ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca      500

cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta      560

gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc      620

catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg      680

gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat      740

aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta      800

acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacgtcga gttatggaca      860

gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa gccctgcaaa      920

gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc aagatttgat      980

caagagacag gatgaggatc gtttcgc atg att gaa caa gat gga ttg cac gca     1034
Met Ile Glu Gln Asp Gly Leu His Ala         
                                          50                  55          

ggt tct ccg gcc gct tgg gtg gag agg cta ttc ggc tat gac tgg gca       1082
Gly Ser Pro Ala Ala Trp Val Glu Arg Leu Phe Gly Tyr Asp Trp Ala           
                60                  65                  70                

caa cag aca atc ggc tgc tct gat gcc gcc gtg ttc cgg ctg tca gcg       1130
Gln Gln Thr Ile Gly Cys Ser Asp Ala Ala Val Phe Arg Leu Ser Ala           
            75                  80                  85                    

cag ggg cgc ccg gtt ctt ttt gtc aag acc gac ctg tcc ggt gcc ctg       1178
Gln Gly Arg Pro Val Leu Phe Val Lys Thr Asp Leu Ser Gly Ala Leu           
        90                  95                  100                       

aat gaa ctc caa gac gag gca gcg cgg cta tcg tgg ctg gcc acg acg       1226
Asn Glu Leu Gln Asp Glu Ala Ala Arg Leu Ser Trp Leu Ala Thr Thr           
    105                 110                 115                           

ggc gtt cct tgc gca gct gtg ctc gac gtt gtc act gaa gcg gga agg       1274
Gly Val Pro Cys Ala Ala Val Leu Asp Val Val Thr Glu Ala Gly Arg           
120                 125                 130                 135           

gac tgg ctg cta ttg ggc gaa gtg ccg ggg cag gat ctc ctg tca tct       1322
Asp Trp Leu Leu Leu Gly Glu Val Pro Gly Gln Asp Leu Leu Ser Ser           
                140                 145                 150               

cac ctt gct cct gcc gag aaa gta tcc atc atg gct gat gca atg cgg       1370
His Leu Ala Pro Ala Glu Lys Val Ser Ile Met Ala Asp Ala Met Arg           
            155                 160                 165                   

cgg ctg cat acg ctt gat ccg gct acc tgc cca ttc gac cac caa gcg       1418
Arg Leu His Thr Leu Asp Pro Ala Thr Cys Pro Phe Asp His Gln Ala           
        170                 175                 180                       

aaa cat cgc atc gag cga gca cgt act cgg atg gaa gcc ggt ctt gtc       1466
Lys His Arg Ile Glu Arg Ala Arg Thr Arg Met Glu Ala Gly Leu Val           
    185                 190                 195                           

gat cag gat gat ctg gac gaa gag cat cag ggg ctc gcg cca gcc gaa       1514
Asp Gln Asp Asp Leu Asp Glu Glu His Gln Gly Leu Ala Pro Ala Glu           
200                 205                 210                 215           

ctg ttc gcc agg ctc aag gcg cgg atg ccc gac ggc gag gat ctc gtc       1562
Leu Phe Ala Arg Leu Lys Ala Arg Met Pro Asp Gly Glu Asp Leu Val           
                220                 225                 230               

gtg acc cac ggc gat gcc tgc ttg ccg aat atc atg gtg gaa aat ggc       1610
Val Thr His Gly Asp Ala Cys Leu Pro Asn Ile Met Val Glu Asn Gly           
            235                 240                 245                   

cgc ttt tct gga ttc atc gac tgt ggc cgg ctg ggt gtg gcg gac cgc       1658
Arg Phe Ser Gly Phe Ile Asp Cys Gly Arg Leu Gly Val Ala Asp Arg           
        250                 255                 260                       

tat cag gac ata gcg ttg gct acc cgt gat att gct gaa gag ctt ggc       1706
Tyr Gln Asp Ile Ala Leu Ala Thr Arg Asp Ile Ala Glu Glu Leu Gly           
    265                 270                 275                           

ggc gaa tgg gct gac cgc ttc ctc gtg ctt tac ggt atc gcc gct ccc       1754
Gly Glu Trp Ala Asp Arg Phe Leu Val Leu Tyr Gly Ile Ala Ala Pro           
280                 285                 290                 295           

gat tcg cag cgc atc gcc ttc tat cgc ctt ctt gac gag ttc ttc tga       1802
Asp Ser Gln Arg Ile Ala Phe Tyr Arg Leu Leu Asp Glu Phe Phe               
                300                 305                 310               

gcgggactct ggggttcgaa attctagtgc attggtaact gtcagaccaa gtttactcat     1862

atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc     1922

tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag     1982

accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct     2042

gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac     2102

caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc     2162

tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg     2222

ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt     2282

tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt     2342

gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc     2402

tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca     2462

gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata     2522

gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg     2582

ggcggagcct atggaaaaac gccagcagtc gagtttgtct ccgaccatca ggcacctgag     2642

tcgctgtctt tttcgtgaca ttcagttcgc tgcgctcacg gctctggcag tgaatggggg     2702

taaatggcac tacaggcgcc ttttatggat tcatgcaagg aaactaccca taatacaaga     2762

aaagcccgtc acgggcttct cagggcgttt tatggcgggt ctgctatgtg gtgctatctg     2822

actttttgct gttcagcagt tcctgccctc tgattttcca gtctgaccac ttcggattat     2882

cccgtctagt acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg     2942

ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct     3002

gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa     3062

gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcag         3118



<210>    SEQ ID No: 20 
<211>    46 
<212>    protena
<213>    sequncia artificial

<220>
<223>    Constructo sinttico 

<400>    20 

Met His His His His His His Leu Glu Gly Ser Asp Leu Glu Ile Cys 
1               5                   10                  15      


Ser Trp Tyr His Gly Ile Arg Ser Leu Ile Arg Leu Leu Thr Lys Pro 
20                  25                  30          


Glu Arg Lys Leu Ser Trp Leu Leu Pro Pro Leu Ser Asn Asn 
35                  40                  45      



<210>    SEQ ID No: 21 
<211>    264
<212>    protena
<213>    sequncia artificial

<220>
<223>    Constructo sinttico

<400>    21 

Met Ile Glu Gln Asp Gly Leu His Ala Gly Ser Pro Ala Ala Trp Val 
1               5                   10                  15      


Glu Arg Leu Phe Gly Tyr Asp Trp Ala Gln Gln Thr Ile Gly Cys Ser 
            20                  25                  30          


Asp Ala Ala Val Phe Arg Leu Ser Ala Gln Gly Arg Pro Val Leu Phe 
        35                  40                  45              


Val Lys Thr Asp Leu Ser Gly Ala Leu Asn Glu Leu Gln Asp Glu Ala 
50                  55                  60                  


Ala Arg Leu Ser Trp Leu Ala Thr Thr Gly Val Pro Cys Ala Ala Val 
65                  70                  75                  80  


Leu Asp Val Val Thr Glu Ala Gly Arg Asp Trp Leu Leu Leu Gly Glu 
                85                  90                  95      


Val Pro Gly Gln Asp Leu Leu Ser Ser His Leu Ala Pro Ala Glu Lys 
100                 105                 110         


Val Ser Ile Met Ala Asp Ala Met Arg Arg Leu His Thr Leu Asp Pro 
        115                 120                 125             


Ala Thr Cys Pro Phe Asp His Gln Ala Lys His Arg Ile Glu Arg Ala 
    130                 135                 140                 


Arg Thr Arg Met Glu Ala Gly Leu Val Asp Gln Asp Asp Leu Asp Glu 
145                 150                 155                 160 


Glu His Gln Gly Leu Ala Pro Ala Glu Leu Phe Ala Arg Leu Lys Ala 
                165                 170                 175     


Arg Met Pro Asp Gly Glu Asp Leu Val Val Thr His Gly Asp Ala Cys 
            180                 185                 190         


Leu Pro Asn Ile Met Val Glu Asn Gly Arg Phe Ser Gly Phe Ile Asp 
        195                 200                 205             


Cys Gly Arg Leu Gly Val Ala Asp Arg Tyr Gln Asp Ile Ala Leu Ala 
    210                 215                 220                 


Thr Arg Asp Ile Ala Glu Glu Leu Gly Gly Glu Trp Ala Asp Arg Phe 
225                 230                 235                 240 


Leu Val Leu Tyr Gly Ile Ala Ala Pro Asp Ser Gln Arg Ile Ala Phe 
245                 250                 255     


Tyr Arg Leu Leu Asp Glu Phe Phe 
            260                 



<210>    SEQ ID No: 22 
<211>    66
<212>    DNA
<213>    sequncia artificial

<220>
<223>    Sequncia para introduo de um RBS e cdon ATG start no gene de Rrp4

<400>    22 

tctagaaata attttgttta actttaagaa ggagatatat atatggctat cgatgcggat       60

atcgcc                                                                  66

<210>    SEQ ID No: 23 
<211>    167 
<212>    DNA
<213>    sequncia artificial 

<220>
<223>    Sequncia End para o gene Rrp4, promotor e cdon Start para o gene de Rrp41

<400>    23 

agatcttaag gtaccctgca ggcttaagtc gaacagaaag taatcgtatt gtacacggcc       60

gcaacttcga aatcaatacg actcactata gggagaccac aacggtttcc catcttagta      120

tattagttta actttaagaa ggagatatac atatggctac gcgtgca                    167



<210>    SEQ ID No: 24 
<211>    162 
<212>    DNA
<213>    sequncia artificial 

<220>
<223>    Sequncia End para o gene de Rrp41, promotor e cdon Start para o gene de Rrp42

<400>    24 

ggatcctaaa ctagtgaatt cgcttaagtc gaacagaaag taatcgtatt gtacacggcc       60

gcataatcga aattaatacg actcactata gggagaccac aacggtttcc catcttagta      120

tattagttaa gtataagaag gagatatacc atgggtgtcg ac                         162



<210>    SEQ ID No: 25 
<211>    23 
<212>    DNA
<213>    sequncia artificial 

<220>
<223>    Sequncia End para o gene de Rrp42

<400>    25 

ctcgagtaag cggccgcaag ctt                                               23



<210>    SEQ ID No: 26
<211>    783 
<212>    DNA
<213>    sequncia artificial

<220>
<223>    Gene sinttico para a protena Rrp4, aa 2 at 262

<220>
<221>    CDS
<222>    (1) ... (783)
<223>    aa 2 at 262 do gene de Rrp4 

<400>    26

aaa cgt atc ttc gta cag aat cgt gaa ctg gtg gtg ccg ggt act ctg         48
Lys Arg Ile Phe Val Gln Asn Arg Glu Leu Val Val Pro Gly Thr Leu           
1               5                   10                  15                

ctg gcc cag ggt cct tat aaa aac ggt cgt ggc act ttc cgt gaa ggt         96
Leu Ala Gln Gly Pro Tyr Lys Asn Gly Arg Gly Thr Phe Arg Glu Gly           
            20                  25                  30                    

agc cgt atc tac tct acc gtt atc ggt ctg gtg gac atc aaa ggc aac        144
Ser Arg Ile Tyr Ser Thr Val Ile Gly Leu Val Asp Ile Lys Gly Asn           
        35                  40                  45                        

acc att cgt gtg att cct ctg gag ggt ccg tac atc ccg gaa gta ggt        192
Thr Ile Arg Val Ile Pro Leu Glu Gly Pro Tyr Ile Pro Glu Val Gly           
    50                  55                  60                            

gac aac gtt atc ggt aaa att gtg gac gtt aag ttc tct agc tgg gtt        240
Asp Asn Val Ile Gly Lys Ile Val Asp Val Lys Phe Ser Ser Trp Val           
65                  70                  75                  80            

gta gac att ggt gcg ccg tat ccg gcc aac ttg aag att cag gac ttc        288
Val Asp Ile Gly Ala Pro Tyr Pro Ala Asn Leu Lys Ile Gln Asp Phe           
                85                  90                  95                

act gat gaa aaa att gat ttg ctg cgt act gac ctt cgt aag ttc ttt        336
Thr Asp Glu Lys Ile Asp Leu Leu Arg Thr Asp Leu Arg Lys Phe Phe           
            100                 105                 110                   

gat att ggt gac att atc tac gct aaa gta aaa gcc atc acc gaa gtt        384
Asp Ile Gly Asp Ile Ile Tyr Ala Lys Val Lys Ala Ile Thr Glu Val           
        115                 120                 125                       

aac aac att gac ctg acg acc aaa ggc atg ccg ttt aac ggt ggt ccg        432
Asn Asn Ile Asp Leu Thr Thr Lys Gly Met Pro Phe Asn Gly Gly Pro           
    130                 135                 140                           

ctg aaa ggt ggt caa atc gtg aaa att acg ccg tct cgt gtg ccg cgt        480
Leu Lys Gly Gly Gln Ile Val Lys Ile Thr Pro Ser Arg Val Pro Arg           
145                 150                 155                 160           

gtt atc ggt cgc ggt ggc agc atg att aac atg att aaa aaa ctg acc        528
Val Ile Gly Arg Gly Gly Ser Met Ile Asn Met Ile Lys Lys Leu Thr           
                165                 170                 175               

atg act cgt att att gtt ggc caa aac ggc tgg att tgg gtg tct ggc        576
Met Thr Arg Ile Ile Val Gly Gln Asn Gly Trp Ile Trp Val Ser Gly           
            180                 185                 190                   

aaa aac gat gcg ctg gaa aaa ctt gct att gag gcc att ctg aaa att        624
Lys Asn Asp Ala Leu Glu Lys Leu Ala Ile Glu Ala Ile Leu Lys Ile           
        195                 200                 205                       

gac aaa gag tct cat acc cgt ggt ctg act gac cgt att aaa gcg ctg        672
Asp Lys Glu Ser His Thr Arg Gly Leu Thr Asp Arg Ile Lys Ala Leu           
    210                 215                 220                           

ctg ctg tcc cgc ctg caa gaa ctg aaa gaa aaa ggt gtt att gaa gag        720
Leu Leu Ser Arg Leu Gln Glu Leu Lys Glu Lys Gly Val Ile Glu Glu           
225                 230                 235                 240           

att ccg aaa ctg gaa gaa gag ccg cag ggc gaa gat gaa gta aac ggc        768
Ile Pro Lys Leu Glu Glu Glu Pro Gln Gly Glu Asp Glu Val Asn Gly           
                245                 250                 255               

aat gac ggt gaa gct                                                    783
Asn Asp Gly Glu Ala                                                       
            260                                                           
    


<210>    SEQ ID No: 27
<211>    261 
<212>    protena
<213>    sequncia artificial

<220>
<223>    constructo sinttico

<400>    27

Lys Arg Ile Phe Val Gln Asn Arg Glu Leu Val Val Pro Gly Thr Leu 
1               5                   10                  15      


Leu Ala Gln Gly Pro Tyr Lys Asn Gly Arg Gly Thr Phe Arg Glu Gly 
            20                  25                  30          


Ser Arg Ile Tyr Ser Thr Val Ile Gly Leu Val Asp Ile Lys Gly Asn 
        35                  40                  45              


Thr Ile Arg Val Ile Pro Leu Glu Gly Pro Tyr Ile Pro Glu Val Gly 
    50                  55                  60                  


Asp Asn Val Ile Gly Lys Ile Val Asp Val Lys Phe Ser Ser Trp Val 
65                  70                  75                  80  


Val Asp Ile Gly Ala Pro Tyr Pro Ala Asn Leu Lys Ile Gln Asp Phe 
                85                  90                  95      


Thr Asp Glu Lys Ile Asp Leu Leu Arg Thr Asp Leu Arg Lys Phe Phe 
            100                 105                 110         


Asp Ile Gly Asp Ile Ile Tyr Ala Lys Val Lys Ala Ile Thr Glu Val 
        115                 120                 125             


Asn Asn Ile Asp Leu Thr Thr Lys Gly Met Pro Phe Asn Gly Gly Pro 
    130                 135                 140                 


Leu Lys Gly Gly Gln Ile Val Lys Ile Thr Pro Ser Arg Val Pro Arg 
145                 150                 155                 160 


Val Ile Gly Arg Gly Gly Ser Met Ile Asn Met Ile Lys Lys Leu Thr 
                165                 170                 175     


Met Thr Arg Ile Ile Val Gly Gln Asn Gly Trp Ile Trp Val Ser Gly 
            180                 185                 190         


Lys Asn Asp Ala Leu Glu Lys Leu Ala Ile Glu Ala Ile Leu Lys Ile 
        195                 200                 205             


Asp Lys Glu Ser His Thr Arg Gly Leu Thr Asp Arg Ile Lys Ala Leu 
    210                 215                 220                 


Leu Leu Ser Arg Leu Gln Glu Leu Lys Glu Lys Gly Val Ile Glu Glu 
225                 230                 235                 240 


Ile Pro Lys Leu Glu Glu Glu Pro Gln Gly Glu Asp Glu Val Asn Gly 
                245                 250                 255     


Asn Asp Gly Glu Ala 
            260     



<210>    SEQ ID No: 28
<211>    tamanho: 732 
<212>    DNA
<213>    sequncia artificial

<220>
<223>    Gene sinttico para a protena Rrp41, aa 3 at 246, duplo mutante R89T e K91S

<220>
<221>    CDS
<222>    (1) ... (732)
<223>    Gene sinttico para a protena Rrp41, aa 3 at 246, duplo mutante R89T e K91S 

<400>    28
    
gag aaa ccg gaa ggt ctg aaa ctg att gat gaa aac ggc cgc cgt att         48
Glu Lys Pro Glu Gly Leu Lys Leu Ile Asp Glu Asn Gly Arg Arg Ile           
1               5                   10                  15                

gac ggt cgt aaa aaa tac gaa ctg cgt ccg att aaa atg gaa gtg ggt         96
Asp Gly Arg Lys Lys Tyr Glu Leu Arg Pro Ile Lys Met Glu Val Gly           
            20                  25                  30                    

gta ctg aaa aac gct aac ggt tcc gcg tac atc gaa tgg ggc aaa aac        144
Val Leu Lys Asn Ala Asn Gly Ser Ala Tyr Ile Glu Trp Gly Lys Asn           
        35                  40                  45                        

aaa att atc gca gcg gtt tac ggt ccg cgt gaa ctc cac ccg aaa cac        192
Lys Ile Ile Ala Ala Val Tyr Gly Pro Arg Glu Leu His Pro Lys His           
    50                  55                  60                            

ctt cag cgt ccg gac cgc gcc atc ctg cgt gta cgt tac aac atg gcc        240
Leu Gln Arg Pro Asp Arg Ala Ile Leu Arg Val Arg Tyr Asn Met Ala           
65                  70                  75                  80            

ccg ttc tct gtt gaa gag act aag agc ccg ggt ccg gac cgt cgt agc        288
Pro Phe Ser Val Glu Glu Thr Lys Ser Pro Gly Pro Asp Arg Arg Ser           
                85                  90                  95                

att gag att tct aaa gta att aaa ggt gcg ctg gaa ccg gca ctt att        336
Ile Glu Ile Ser Lys Val Ile Lys Gly Ala Leu Glu Pro Ala Leu Ile           
            100                 105                 110                   

ctg gaa atg ttc ccg cgt act gct att gat gta ttc atc gaa gtt ctg        384
Leu Glu Met Phe Pro Arg Thr Ala Ile Asp Val Phe Ile Glu Val Leu           
        115                 120                 125                       

caa gcg gat gcc ggt acg cgc gtt gca ggt atc acc gcg gct agc ctg        432
Gln Ala Asp Ala Gly Thr Arg Val Ala Gly Ile Thr Ala Ala Ser Leu           
    130                 135                 140                           

gca ctg gcc gac gct ggt att ccg atg cgc gac ctg gtt gcc gct tgt        480
Ala Leu Ala Asp Ala Gly Ile Pro Met Arg Asp Leu Val Ala Ala Cys           
145                 150                 155                 160           

gcg gcg ggt aaa atc gaa ggc gaa att gtt ctg gac ctg aac aaa gaa        528
Ala Ala Gly Lys Ile Glu Gly Glu Ile Val Leu Asp Leu Asn Lys Glu           
                165                 170                 175               

gaa gat aac tac ggc gaa gcg gac gtg ccg gta gct atc atg ccg ctg        576
Glu Asp Asn Tyr Gly Glu Ala Asp Val Pro Val Ala Ile Met Pro Leu           
            180                 185                 190                   

aag aac gat att acc ctg ctg caa atg gat ggt tac ctg acc aag gac        624
Lys Asn Asp Ile Thr Leu Leu Gln Met Asp Gly Tyr Leu Thr Lys Asp           
        195                 200                 205                       

gag ttc atc gag gcg gtt aaa ctg gct att aaa ggt gct aag gcg gtg        672
Glu Phe Ile Glu Ala Val Lys Leu Ala Ile Lys Gly Ala Lys Ala Val           
    210                 215                 220                           

tat cag aaa cag cgt gaa gca ctg aaa gaa aag tat ctg aaa att gcc        720
Tyr Gln Lys Gln Arg Glu Ala Leu Lys Glu Lys Tyr Leu Lys Ile Ala           
225                 230                 235                 240           

cag gaa gtt gag                                                        732
Gln Glu Val Glu                                                           




<210>    SEQ ID No: 29
<211>    244 
<212>    protena
<213>    sequncia artificial

<220>
<223>    Constructo sinttico

<400>    29

Glu Lys Pro Glu Gly Leu Lys Leu Ile Asp Glu Asn Gly Arg Arg Ile 
1               5                   10                  15      


Asp Gly Arg Lys Lys Tyr Glu Leu Arg Pro Ile Lys Met Glu Val Gly 
            20                  25                  30          


Val Leu Lys Asn Ala Asn Gly Ser Ala Tyr Ile Glu Trp Gly Lys Asn 
        35                  40                  45              


Lys Ile Ile Ala Ala Val Tyr Gly Pro Arg Glu Leu His Pro Lys His 
    50                  55                  60                  


Leu Gln Arg Pro Asp Arg Ala Ile Leu Arg Val Arg Tyr Asn Met Ala 
65                  70                  75                  80  


Pro Phe Ser Val Glu Glu Thr Lys Ser Pro Gly Pro Asp Arg Arg Ser 
                85                  90                  95      


Ile Glu Ile Ser Lys Val Ile Lys Gly Ala Leu Glu Pro Ala Leu Ile 
            100                 105                 110         


Leu Glu Met Phe Pro Arg Thr Ala Ile Asp Val Phe Ile Glu Val Leu 
115                 120                 125             


Gln Ala Asp Ala Gly Thr Arg Val Ala Gly Ile Thr Ala Ala Ser Leu 
    130                 135                 140                 


Ala Leu Ala Asp Ala Gly Ile Pro Met Arg Asp Leu Val Ala Ala Cys 
145                 150                 155                 160 


Ala Ala Gly Lys Ile Glu Gly Glu Ile Val Leu Asp Leu Asn Lys Glu 
                165                 170                 175     


Glu Asp Asn Tyr Gly Glu Ala Asp Val Pro Val Ala Ile Met Pro Leu 
180                 185                 190         


Lys Asn Asp Ile Thr Leu Leu Gln Met Asp Gly Tyr Leu Thr Lys Asp 
        195                 200                 205             


Glu Phe Ile Glu Ala Val Lys Leu Ala Ile Lys Gly Ala Lys Ala Val 
    210                 215                 220                 


Tyr Gln Lys Gln Arg Glu Ala Leu Lys Glu Lys Tyr Leu Lys Ile Ala 
225                 230                 235                 240 


Gln Glu Val Glu 


<210> SEQ ID No: 30
<211> 819 
<212> DNA
<213> sequncia artificial

<220>
<223> Gene sinttido para a protena Rrp42, aa 2 at 274

<220>
<221> CDS
<222> (1) ... (819)
<223> Protena Rrp42, aa 1 at 274

<400> 30

tct gat aat gaa atc gtg gcg ggt att atg cgc gac cat att att aac         48
Ser Asp Asn Glu Ile Val Ala Gly Ile Met Arg Asp His Ile Ile Asn           
1               5                   10                  15                

ctg ctt aag gaa ggt aaa cgt att gac gac cgc ggt ttc gaa gat tac         96
Leu Leu Lys Glu Gly Lys Arg Ile Asp Asp Arg Gly Phe Glu Asp Tyr           
            20                  25                  30                    

cgc ccg att gaa att gag gta ggt gtt att gaa aaa gcg gag ggt agc        144
Arg Pro Ile Glu Ile Glu Val Gly Val Ile Glu Lys Ala Glu Gly Ser           
        35                  40                  45                        

gcg ctg gtg aaa ctg ggt agc act cag gtt ctg gtg ggt att aaa acc        192
Ala Leu Val Lys Leu Gly Ser Thr Gln Val Leu Val Gly Ile Lys Thr           
    50                  55                  60                            

tct ctg ggc gag ccg ttt ccg gac acc ccg aac atg ggc gtg atg acc        240
Ser Leu Gly Glu Pro Phe Pro Asp Thr Pro Asn Met Gly Val Met Thr           
65                  70                  75                  80            

acc aat gtt gaa ctt gtt ccg ctg gcg tct ccg act ttc gaa ccg ggc        288
Thr Asn Val Glu Leu Val Pro Leu Ala Ser Pro Thr Phe Glu Pro Gly           
                85                  90                  95                

ccg ccg gac gag cgt gct atc gaa ctg gca cgt gtt att gac cgt ggt        336
Pro Pro Asp Glu Arg Ala Ile Glu Leu Ala Arg Val Ile Asp Arg Gly           
            100                 105                 110                   

att cgt gag tcc aaa gca ctg aat ctg gaa aaa atg gtt atc gtg ccg        384
Ile Arg Glu Ser Lys Ala Leu Asn Leu Glu Lys Met Val Ile Val Pro           
        115                 120                 125                       

ggc aaa att gtt cgc gta gtt ttt atc gac gtt cac gtt ctt gac cac        432
Gly Lys Ile Val Arg Val Val Phe Ile Asp Val His Val Leu Asp His           
    130                 135                 140                           

gac ggt aac ctg atg gac gcc atc ggt att gca gct atc gcc gca ctg        480
Asp Gly Asn Leu Met Asp Ala Ile Gly Ile Ala Ala Ile Ala Ala Leu           
145                 150                 155                 160           

ctg aac gca cgt gtt ccg aaa gtt cgc tac aac gaa gaa acg ggt gaa        528
Leu Asn Ala Arg Val Pro Lys Val Arg Tyr Asn Glu Glu Thr Gly Glu           
                165                 170                 175               

gtt gaa acc ctg gat gaa act gaa ccg ctg ccg gtt gaa aag att ccg        576
Val Glu Thr Leu Asp Glu Thr Glu Pro Leu Pro Val Glu Lys Ile Pro           
            180                 185                 190                   

gtt ccg gtt act ttc gct aaa att ggt aat atc ctg gta gtt gac ccg        624
Val Pro Val Thr Phe Ala Lys Ile Gly Asn Ile Leu Val Val Asp Pro           
        195                 200                 205                       

agc ctg gat gaa gag ctc gtg atg gac ggc aaa att acc att acc act        672
Ser Leu Asp Glu Glu Leu Val Met Asp Gly Lys Ile Thr Ile Thr Thr           
    210                 215                 220                           

gac gaa acc ggc cat att tct gcc gtt cag aaa tct gaa ggt ggc gct        720
Asp Glu Thr Gly His Ile Ser Ala Val Gln Lys Ser Glu Gly Gly Ala           
225                 230                 235                 240           

ttc aaa ctg gaa gaa gta atg tac gcg gtg gaa act gca ttc aag aag        768
Phe Lys Leu Glu Glu Val Met Tyr Ala Val Glu Thr Ala Phe Lys Lys           
                245                 250                 255               

gcg gaa gaa atc cgt aaa ctg att ctg gaa gct gtt gag aag gcc aaa        816
Ala Glu Glu Ile Arg Lys Leu Ile Leu Glu Ala Val Glu Lys Ala Lys           
            260                 265                 270                   

cag                                                                    819
Gln                                                                       




<210>    SEQ ID No: 31
<211>    273 
<212>    protena
<213>    sequncia artificial

<220>
<223>    Constructo sinttico

<400>    31

Ser Asp Asn Glu Ile Val Ala Gly Ile Met Arg Asp His Ile Ile Asn 
1               5                   10                  15      


Leu Leu Lys Glu Gly Lys Arg Ile Asp Asp Arg Gly Phe Glu Asp Tyr 
            20                  25                  30          


Arg Pro Ile Glu Ile Glu Val Gly Val Ile Glu Lys Ala Glu Gly Ser 
        35                  40                  45              


Ala Leu Val Lys Leu Gly Ser Thr Gln Val Leu Val Gly Ile Lys Thr 
    50                  55                  60                  


Ser Leu Gly Glu Pro Phe Pro Asp Thr Pro Asn Met Gly Val Met Thr 
65                  70                  75                  80  


Thr Asn Val Glu Leu Val Pro Leu Ala Ser Pro Thr Phe Glu Pro Gly 
                85                  90                  95      


Pro Pro Asp Glu Arg Ala Ile Glu Leu Ala Arg Val Ile Asp Arg Gly 
            100                 105                 110         


Ile Arg Glu Ser Lys Ala Leu Asn Leu Glu Lys Met Val Ile Val Pro 
        115                 120                 125             


Gly Lys Ile Val Arg Val Val Phe Ile Asp Val His Val Leu Asp His 
    130                 135                 140                 


Asp Gly Asn Leu Met Asp Ala Ile Gly Ile Ala Ala Ile Ala Ala Leu 
145                 150                 155                 160 


Leu Asn Ala Arg Val Pro Lys Val Arg Tyr Asn Glu Glu Thr Gly Glu 
                165                 170                 175     


Val Glu Thr Leu Asp Glu Thr Glu Pro Leu Pro Val Glu Lys Ile Pro 
            180                 185                 190         


Val Pro Val Thr Phe Ala Lys Ile Gly Asn Ile Leu Val Val Asp Pro 
        195                 200                 205             


Ser Leu Asp Glu Glu Leu Val Met Asp Gly Lys Ile Thr Ile Thr Thr 
    210                 215                 220                 


Asp Glu Thr Gly His Ile Ser Ala Val Gln Lys Ser Glu Gly Gly Ala 
225                 230                 235                 240 


Phe Lys Leu Glu Glu Val Met Tyr Ala Val Glu Thr Ala Phe Lys Lys 
                245                 250                 255     


Ala Glu Glu Ile Arg Lys Leu Ile Leu Glu Ala Val Glu Lys Ala Lys 
            260                 265                 270         


Gln 



<210>    SEQ ID No: 32
<211>    2752 
<212>    DNA
<213>    sequncia artificial

<220>
<223>    Gene sinttico para os trs componentes do complexo do exossomo de P. abyssi

<220>
<221>    misc_feature
<222>    (1) ... (6)
<223>    stio de restrio XbaI

<220>
<221>    CDS
<210>    (43) ... (858)
<210>    protena Rrp4 de Pyrococcus abyssi

<220>
<221>    misc_feature
<222>    (49) ... (63)
<223>    stios de restrio ClaI e EcoRV

<220>
<221>    promotor
<222>    (922) ... (942)
<223>    promotor T7 para o gene de Rrp41

<220>
<221>    misc_feature
<222>    (922) ... (942)
<223>    Stio Bgl II, cdon Stop, stios de restrio KpnI e PstI

<220>
<221>    misc_feature
<222>    (999) ... (1013)
<223>    stios de restrio NdeI e MluI

<220>
<221>    CDS
<222>    (1002) ... (1757)
<223>    Protena mutante dupla R89T K91S de Rrp41 de Pyrococcus abyssi

<220>
<221>    nome: misc_feature
<222>    (1749) ... (1769)
<223>    stio BamHI site, cdon Stop,  stios de restrio SpeI e  EcoRI

<220>
<221>    promotor
<222>    (1822) ... (1842)
<223>    promotor T7 para o gene de Rrp42

<220>
<221>    misc_feature
<222>    (1897) ... (1910)
<223> stios de restrio Ncol e SaII

<220>
<221>    CDS
<222>    (1899) ... (2738)
<223>    protena Rrp42 de Pyrococcus abyssi

<220>
<221>    misc_feature
<222>    (2730) ... (2752)
<223>    stio XhoI, cdon Stop,  stios de restrio NotI e  HindIII

<400> 32

tctagaaata attttgttta actttaagaa ggagatatat at atg gct atc gat          54
                                               Met Ala Ile Asp            
                                               1                          

gcg gat atc gcc aaa cgt atc ttc gta cag aat cgt gaa ctg gtg gtg        102
Ala Asp Ile Ala Lys Arg Ile Phe Val Gln Asn Arg Glu Leu Val Val           
5                   10                  15                  20            

ccg ggt act ctg ctg gcc cag ggt cct tat aaa aac ggt cgt ggc act        150
Pro Gly Thr Leu Leu Ala Gln Gly Pro Tyr Lys Asn Gly Arg Gly Thr           
                25                  30                  35                

ttc cgt gaa ggt agc cgt atc tac tct acc gtt atc ggt ctg gtg gac        198
Phe Arg Glu Gly Ser Arg Ile Tyr Ser Thr Val Ile Gly Leu Val Asp           
            40                  45                  50                    

atc aaa ggc aac acc att cgt gtg att cct ctg gag ggt ccg tac atc        246
Ile Lys Gly Asn Thr Ile Arg Val Ile Pro Leu Glu Gly Pro Tyr Ile           
        55                  60                  65                        

ccg gaa gta ggt gac aac gtt atc ggt aaa att gtg gac gtt aag ttc        294
Pro Glu Val Gly Asp Asn Val Ile Gly Lys Ile Val Asp Val Lys Phe           
    70                  75                  80                            

tct agc tgg gtt gta gac att ggt gcg ccg tat ccg gcc aac ttg aag        342
Ser Ser Trp Val Val Asp Ile Gly Ala Pro Tyr Pro Ala Asn Leu Lys           
85                  90                  95                  100           

att cag gac ttc act gat gaa aaa att gat ttg ctg cgt act gac ctt        390
Ile Gln Asp Phe Thr Asp Glu Lys Ile Asp Leu Leu Arg Thr Asp Leu           
                105                 110                 115               

cgt aag ttc ttt gat att ggt gac att atc tac gct aaa gta aaa gcc        438
Arg Lys Phe Phe Asp Ile Gly Asp Ile Ile Tyr Ala Lys Val Lys Ala           
            120                 125                 130                   

atc acc gaa gtt aac aac att gac ctg acg acc aaa ggc atg ccg ttt        486
Ile Thr Glu Val Asn Asn Ile Asp Leu Thr Thr Lys Gly Met Pro Phe           
        135                 140                 145                       

aac ggt ggt ccg ctg aaa ggt ggt caa atc gtg aaa att acg ccg tct        534
Asn Gly Gly Pro Leu Lys Gly Gly Gln Ile Val Lys Ile Thr Pro Ser           
    150                 155                 160                           

cgt gtg ccg cgt gtt atc ggt cgc ggt ggc agc atg att aac atg att        582
Arg Val Pro Arg Val Ile Gly Arg Gly Gly Ser Met Ile Asn Met Ile           
165                 170                 175                 180           

aaa aaa ctg acc atg act cgt att att gtt ggc caa aac ggc tgg att        630
Lys Lys Leu Thr Met Thr Arg Ile Ile Val Gly Gln Asn Gly Trp Ile           
                185                 190                 195               

tgg gtg tct ggc aaa aac gat gcg ctg gaa aaa ctt gct att gag gcc        678
Trp Val Ser Gly Lys Asn Asp Ala Leu Glu Lys Leu Ala Ile Glu Ala           
            200                 205                 210                   

att ctg aaa att gac aaa gag tct cat acc cgt ggt ctg act gac cgt        726
Ile Leu Lys Ile Asp Lys Glu Ser His Thr Arg Gly Leu Thr Asp Arg           
        215                 220                 225                       

att aaa gcg ctg ctg ctg tcc cgc ctg caa gaa ctg aaa gaa aaa ggt        774
Ile Lys Ala Leu Leu Leu Ser Arg Leu Gln Glu Leu Lys Glu Lys Gly           
    230                 235                 240                           

gtt att gaa gag att ccg aaa ctg gaa gaa gag ccg cag ggc gaa gat        822
Val Ile Glu Glu Ile Pro Lys Leu Glu Glu Glu Pro Gln Gly Glu Asp           
245                 250                 255                 260           

gaa gta aac ggc aat gac ggt gaa gct aga tct taa ggtaccctgc             868
Glu Val Asn Gly Asn Asp Gly Glu Ala Arg Ser                               
                265                 270                                   

aggcttaagt cgaacagaaa gtaatcgtat tgtacacggc cgcaacttcg aaatcaatac      928

gactcactat agggagacca caacggtttc ccatcttagt atattagttt aactttaaga      988

aggagatata cat atg gct acg cgt gca gag aaa ccg gaa ggt ctg aaa        1037
Met Ala Thr Arg Ala Glu Lys Pro Glu Gly Leu Lys            
                           275                 280                        

ctg att gat gaa aac ggc cgc cgt att gac ggt cgt aaa aaa tac gaa       1085
Leu Ile Asp Glu Asn Gly Arg Arg Ile Asp Gly Arg Lys Lys Tyr Glu           
    285                 290                 295                           

ctg cgt ccg att aaa atg gaa gtg ggt gta ctg aaa aac gct aac ggt       1133
Leu Arg Pro Ile Lys Met Glu Val Gly Val Leu Lys Asn Ala Asn Gly           
300                 305                 310                 315           

tcc gcg tac atc gaa tgg ggc aaa aac aaa att atc gca gcg gtt tac       1181
Ser Ala Tyr Ile Glu Trp Gly Lys Asn Lys Ile Ile Ala Ala Val Tyr           
                320                 325                 330               

ggt ccg cgt gaa ctc cac ccg aaa cac ctt cag cgt ccg gac cgc gcc       1229
Gly Pro Arg Glu Leu His Pro Lys His Leu Gln Arg Pro Asp Arg Ala           
            335                 340                 345                   

atc ctg cgt gta cgt tac aac atg gcc ccg ttc tct gtt gaa gag act       1277
Ile Leu Arg Val Arg Tyr Asn Met Ala Pro Phe Ser Val Glu Glu Thr           
        350                 355                 360                       

aag agc ccg ggt ccg gac cgt cgt agc att gag att tct aaa gta att       1325
Lys Ser Pro Gly Pro Asp Arg Arg Ser Ile Glu Ile Ser Lys Val Ile           
    365                 370                 375                           

aaa ggt gcg ctg gaa ccg gca ctt att ctg gaa atg ttc ccg cgt act       1373
Lys Gly Ala Leu Glu Pro Ala Leu Ile Leu Glu Met Phe Pro Arg Thr           
380                 385                 390                 395           

gct att gat gta ttc atc gaa gtt ctg caa gcg gat gcc ggt acg cgc       1421
Ala Ile Asp Val Phe Ile Glu Val Leu Gln Ala Asp Ala Gly Thr Arg           
                400                 405                 410               

gtt gca ggt atc acc gcg gct agc ctg gca ctg gcc gac gct ggt att       1469
Val Ala Gly Ile Thr Ala Ala Ser Leu Ala Leu Ala Asp Ala Gly Ile           
            415                 420                 425                   

ccg atg cgc gac ctg gtt gcc gct tgt gcg gcg ggt aaa atc gaa ggc       1517
Pro Met Arg Asp Leu Val Ala Ala Cys Ala Ala Gly Lys Ile Glu Gly           
        430                 435                 440                       

gaa att gtt ctg gac ctg aac aaa gaa gaa gat aac tac ggc gaa gcg       1565
Glu Ile Val Leu Asp Leu Asn Lys Glu Glu Asp Asn Tyr Gly Glu Ala           
    445                 450                 455                           

gac gtg ccg gta gct atc atg ccg ctg aag aac gat att acc ctg ctg       1613
Asp Val Pro Val Ala Ile Met Pro Leu Lys Asn Asp Ile Thr Leu Leu           
460                 465                 470                 475           

caa atg gat ggt tac ctg acc aag gac gag ttc atc gag gcg gtt aaa       1661
Gln Met Asp Gly Tyr Leu Thr Lys Asp Glu Phe Ile Glu Ala Val Lys           
                480                 485                 490               

ctg gct att aaa ggt gct aag gcg gtg tat cag aaa cag cgt gaa gca       1709
Leu Ala Ile Lys Gly Ala Lys Ala Val Tyr Gln Lys Gln Arg Glu Ala           
            495                 500                 505                   

ctg aaa gaa aag tat ctg aaa att gcc cag gaa gtt gag gga tcc taa       1757
Leu Lys Glu Lys Tyr Leu Lys Ile Ala Gln Glu Val Glu Gly Ser               
        510                 515                 520                       

actagtgaat tcgcttaagt cgaacagaaa gtaatcgtat tgtacacggc cgcataatcg     1817

aaattaatac gactcactat agggagacca caacggtttc ccatcttagt atattagtta     1877

agtataagaa ggagatatac c atg ggt gtc gac tct gat aat gaa atc gtg       1928
                        Met Gly Val Asp Ser Asp Asn Glu Ile Val           
                                525                 530                   

gcg ggt att atg cgc gac cat att att aac ctg ctt aag gaa ggt aaa       1976
Ala Gly Ile Met Arg Asp His Ile Ile Asn Leu Leu Lys Glu Gly Lys           
        535                 540                 545                       

cgt att gac gac cgc ggt ttc gaa gat tac cgc ccg att gaa att gag       2024
Arg Ile Asp Asp Arg Gly Phe Glu Asp Tyr Arg Pro Ile Glu Ile Glu           
    550                 555                 560                           

gta ggt gtt att gaa aaa gcg gag ggt agc gcg ctg gtg aaa ctg ggt       2072
Val Gly Val Ile Glu Lys Ala Glu Gly Ser Ala Leu Val Lys Leu Gly           
565                 570                 575                 580           

agc act cag gtt ctg gtg ggt att aaa acc tct ctg ggc gag ccg ttt       2120
Ser Thr Gln Val Leu Val Gly Ile Lys Thr Ser Leu Gly Glu Pro Phe           
                585                 590                 595               

ccg gac acc ccg aac atg ggc gtg atg acc acc aat gtt gaa ctt gtt       2168
Pro Asp Thr Pro Asn Met Gly Val Met Thr Thr Asn Val Glu Leu Val           
            600                 605                 610                   

ccg ctg gcg tct ccg act ttc gaa ccg ggc ccg ccg gac gag cgt gct       2216
Pro Leu Ala Ser Pro Thr Phe Glu Pro Gly Pro Pro Asp Glu Arg Ala           
        615                 620                 625                       

atc gaa ctg gca cgt gtt att gac cgt ggt att cgt gag tcc aaa gca       2264
Ile Glu Leu Ala Arg Val Ile Asp Arg Gly Ile Arg Glu Ser Lys Ala           
    630                 635                 640                           

ctg aat ctg gaa aaa atg gtt atc gtg ccg ggc aaa att gtt cgc gta       2312
Leu Asn Leu Glu Lys Met Val Ile Val Pro Gly Lys Ile Val Arg Val           
645                 650                 655                 660           

gtt ttt atc gac gtt cac gtt ctt gac cac gac ggt aac ctg atg gac       2360
Val Phe Ile Asp Val His Val Leu Asp His Asp Gly Asn Leu Met Asp           
                665                 670                 675               

gcc atc ggt att gca gct atc gcc gca ctg ctg aac gca cgt gtt ccg       2408
Ala Ile Gly Ile Ala Ala Ile Ala Ala Leu Leu Asn Ala Arg Val Pro           
            680                 685                 690                   

aaa gtt cgc tac aac gaa gaa acg ggt gaa gtt gaa acc ctg gat gaa       2456
Lys Val Arg Tyr Asn Glu Glu Thr Gly Glu Val Glu Thr Leu Asp Glu           
        695                 700                 705                       

act gaa ccg ctg ccg gtt gaa aag att ccg gtt ccg gtt act ttc gct       2504
Thr Glu Pro Leu Pro Val Glu Lys Ile Pro Val Pro Val Thr Phe Ala           
    710                 715                 720                           

aaa att ggt aat atc ctg gta gtt gac ccg agc ctg gat gaa gag ctc       2552
Lys Ile Gly Asn Ile Leu Val Val Asp Pro Ser Leu Asp Glu Glu Leu           
725                 730                 735                 740           

gtg atg gac ggc aaa att acc att acc act gac gaa acc ggc cat att       2600
Val Met Asp Gly Lys Ile Thr Ile Thr Thr Asp Glu Thr Gly His Ile           
                745                 750                 755               

tct gcc gtt cag aaa tct gaa ggt ggc gct ttc aaa ctg gaa gaa gta       2648
Ser Ala Val Gln Lys Ser Glu Gly Gly Ala Phe Lys Leu Glu Glu Val           
            760                 765                 770                   

atg tac gcg gtg gaa act gca ttc aag aag gcg gaa gaa atc cgt aaa       2696
Met Tyr Ala Val Glu Thr Ala Phe Lys Lys Ala Glu Glu Ile Arg Lys           
        775                 780                 785                       

ctg att ctg gaa gct gtt gag aag gcc aaa cag ctc gag taa               2738
Leu Ile Leu Glu Ala Val Glu Lys Ala Lys Gln Leu Glu                       
    790                 795                 800                           

gcggccgcaa gctt                                                       2752



<210> SEQ ID No: 33
<211> 271 
<212> protena
<213> sequncia artificial

<220>
<223> Constructo sinttico

<400> 33

Met Ala Ile Asp Ala Asp Ile Ala Lys Arg Ile Phe Val Gln Asn Arg 
1               5                   10                  15      


Glu Leu Val Val Pro Gly Thr Leu Leu Ala Gln Gly Pro Tyr Lys Asn 
            20                  25                  30          


Gly Arg Gly Thr Phe Arg Glu Gly Ser Arg Ile Tyr Ser Thr Val Ile 
        35                  40                  45              


Gly Leu Val Asp Ile Lys Gly Asn Thr Ile Arg Val Ile Pro Leu Glu 
    50                  55                  60                  


Gly Pro Tyr Ile Pro Glu Val Gly Asp Asn Val Ile Gly Lys Ile Val 
65                  70                  75                  80  


Asp Val Lys Phe Ser Ser Trp Val Val Asp Ile Gly Ala Pro Tyr Pro 
                85                  90                  95      


Ala Asn Leu Lys Ile Gln Asp Phe Thr Asp Glu Lys Ile Asp Leu Leu 
            100                 105                 110         


Arg Thr Asp Leu Arg Lys Phe Phe Asp Ile Gly Asp Ile Ile Tyr Ala 
        115                 120                 125             


Lys Val Lys Ala Ile Thr Glu Val Asn Asn Ile Asp Leu Thr Thr Lys 
    130                 135                 140                 


Gly Met Pro Phe Asn Gly Gly Pro Leu Lys Gly Gly Gln Ile Val Lys 
145                 150                 155                 160 


Ile Thr Pro Ser Arg Val Pro Arg Val Ile Gly Arg Gly Gly Ser Met 
                165                 170                 175     


Ile Asn Met Ile Lys Lys Leu Thr Met Thr Arg Ile Ile Val Gly Gln 
            180                 185                 190         


Asn Gly Trp Ile Trp Val Ser Gly Lys Asn Asp Ala Leu Glu Lys Leu 
        195                 200                 205             


Ala Ile Glu Ala Ile Leu Lys Ile Asp Lys Glu Ser His Thr Arg Gly 
    210                 215                 220                 


Leu Thr Asp Arg Ile Lys Ala Leu Leu Leu Ser Arg Leu Gln Glu Leu 
225                 230                 235                 240 


Lys Glu Lys Gly Val Ile Glu Glu Ile Pro Lys Leu Glu Glu Glu Pro 
                245                 250                 255     


Gln Gly Glu Asp Glu Val Asn Gly Asn Asp Gly Glu Ala Arg Ser 
            260                 265                 270     




<210>    SEQ ID No: 34
<211>    251 
<212>    protena
<213>    sequncia artificial

<220>
<223>    Constructo artificial

<400>    34

Met Ala Thr Arg Ala Glu Lys Pro Glu Gly Leu Lys Leu Ile Asp Glu 
1               5                   10                  15      


Asn Gly Arg Arg Ile Asp Gly Arg Lys Lys Tyr Glu Leu Arg Pro Ile 
            20                  25                  30          


Lys Met Glu Val Gly Val Leu Lys Asn Ala Asn Gly Ser Ala Tyr Ile 
        35                  40                  45              


Glu Trp Gly Lys Asn Lys Ile Ile Ala Ala Val Tyr Gly Pro Arg Glu 
    50                  55                  60                  


Leu His Pro Lys His Leu Gln Arg Pro Asp Arg Ala Ile Leu Arg Val 
65                  70                  75                  80  


Arg Tyr Asn Met Ala Pro Phe Ser Val Glu Glu Thr Lys Ser Pro Gly 
                85                  90                  95      


Pro Asp Arg Arg Ser Ile Glu Ile Ser Lys Val Ile Lys Gly Ala Leu 
            100                 105                 110         


Glu Pro Ala Leu Ile Leu Glu Met Phe Pro Arg Thr Ala Ile Asp Val 
115                 120                 125             


Phe Ile Glu Val Leu Gln Ala Asp Ala Gly Thr Arg Val Ala Gly Ile 
    130                 135                 140                 


Thr Ala Ala Ser Leu Ala Leu Ala Asp Ala Gly Ile Pro Met Arg Asp 
145                 150                 155                 160 


Leu Val Ala Ala Cys Ala Ala Gly Lys Ile Glu Gly Glu Ile Val Leu 
                165                 170                 175     


Asp Leu Asn Lys Glu Glu Asp Asn Tyr Gly Glu Ala Asp Val Pro Val 
180                 185                 190         


Ala Ile Met Pro Leu Lys Asn Asp Ile Thr Leu Leu Gln Met Asp Gly 
        195                 200                 205             


Tyr Leu Thr Lys Asp Glu Phe Ile Glu Ala Val Lys Leu Ala Ile Lys 
    210                 215                 220                 


Gly Ala Lys Ala Val Tyr Gln Lys Gln Arg Glu Ala Leu Lys Glu Lys 
225                 230                 235                 240 


Tyr Leu Lys Ile Ala Gln Glu Val Glu Gly Ser 
                245                 250     





<210>    SEQ ID No: 35
<211>    279 
<212>    protena
<213>    sequncia artificial

<220>
<223>    Constructo artificial

<400>    35

Met Gly Val Asp Ser Asp Asn Glu Ile Val Ala Gly Ile Met Arg Asp 
1               5                   10                  15      


His Ile Ile Asn Leu Leu Lys Glu Gly Lys Arg Ile Asp Asp Arg Gly 
            20                  25                  30          


Phe Glu Asp Tyr Arg Pro Ile Glu Ile Glu Val Gly Val Ile Glu Lys 
        35                  40                  45              


Ala Glu Gly Ser Ala Leu Val Lys Leu Gly Ser Thr Gln Val Leu Val 
    50                  55                  60                  


Gly Ile Lys Thr Ser Leu Gly Glu Pro Phe Pro Asp Thr Pro Asn Met 
65                  70                  75                  80  


Gly Val Met Thr Thr Asn Val Glu Leu Val Pro Leu Ala Ser Pro Thr 
                85                  90                  95      


Phe Glu Pro Gly Pro Pro Asp Glu Arg Ala Ile Glu Leu Ala Arg Val 
100                 105                 110         


Ile Asp Arg Gly Ile Arg Glu Ser Lys Ala Leu Asn Leu Glu Lys Met 
115                 120                 125             


Val Ile Val Pro Gly Lys Ile Val Arg Val Val Phe Ile Asp Val His 
    130                 135                 140                 


Val Leu Asp His Asp Gly Asn Leu Met Asp Ala Ile Gly Ile Ala Ala 
145                 150                 155                 160 


Ile Ala Ala Leu Leu Asn Ala Arg Val Pro Lys Val Arg Tyr Asn Glu 
                165                 170                 175     


Glu Thr Gly Glu Val Glu Thr Leu Asp Glu Thr Glu Pro Leu Pro Val 
            180                 185                 190         


Glu Lys Ile Pro Val Pro Val Thr Phe Ala Lys Ile Gly Asn Ile Leu 
        195                 200                 205             


Val Val Asp Pro Ser Leu Asp Glu Glu Leu Val Met Asp Gly Lys Ile 
    210                 215                 220                 


Thr Ile Thr Thr Asp Glu Thr Gly His Ile Ser Ala Val Gln Lys Ser 
225                 230                 235                 240 


Glu Gly Gly Ala Phe Lys Leu Glu Glu Val Met Tyr Ala Val Glu Thr 
                245                 250                 255     


Ala Phe Lys Lys Ala Glu Glu Ile Arg Lys Leu Ile Leu Glu Ala Val 
            260                 265                 270         


Glu Lys Ala Lys Gln Leu Glu 
        275                 



<210>    SEQ ID No: 36
<211>    5754 
<212>    DNA
<213>    sequncia artificial

<220>
<223>    Plasmdeo pMRKA-EXO para expresso de protenas recombinantes em fuso com protenas do exossomo de Pyrococcus abyssi

<400>    36

gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct       60

agaaataatt ttgtttaact ttaagaagga gatatatata tggctatcga tgcggatatc      120

gccaaacgta tcttcgtaca gaatcgtgaa ctggtggtgc cgggtactct gctggcccag      180

ggtccttata aaaacggtcg tggcactttc cgtgaaggta gccgtatcta ctctaccgtt      240

atcggtctgg tggacatcaa aggcaacacc attcgtgtga ttcctctgga gggtccgtac      300

atcccggaag taggtgacaa cgttatcggt aaaattgtgg acgttaagtt ctctagctgg      360

gttgtagaca ttggtgcgcc gtatccggcc aacttgaaga ttcaggactt cactgatgaa      420

aaaattgatt tgctgcgtac tgaccttcgt aagttctttg atattggtga cattatctac      480

gctaaagtaa aagccatcac cgaagttaac aacattgacc tgacgaccaa aggcatgccg      540

tttaacggtg gtccgctgaa aggtggtcaa atcgtgaaaa ttacgccgtc tcgtgtgccg      600

cgtgttatcg gtcgcggtgg cagcatgatt aacatgatta aaaaactgac catgactcgt      660

attattgttg gccaaaacgg ctggatttgg gtgtctggca aaaacgatgc gctggaaaaa      720

cttgctattg aggccattct gaaaattgac aaagagtctc atacccgtgg tctgactgac      780

cgtattaaag cgctgctgct gtcccgcctg caagaactga aagaaaaagg tgttattgaa      840

gagattccga aactggaaga agagccgcag ggcgaagatg aagtaaacgg caatgacggt      900

gaagctagat cttaaggtac cctgcaggct taagtcgaac agaaagtaat cgtattgtac      960

acggccgcaa cttcgaaatc aatacgactc actataggga gaccacaacg gtttcccatc     1020

ttagtatatt agtttaactt taagaaggag atatacatat ggctacgcgt gcagagaaac     1080

cggaaggtct gaaactgatt gatgaaaacg gccgccgtat tgacggtcgt aaaaaatacg     1140

aactgcgtcc gattaaaatg gaagtgggtg tactgaaaaa cgctaacggt tccgcgtaca     1200

tcgaatgggg caaaaacaaa attatcgcag cggtttacgg tccgcgtgaa ctccacccga     1260

aacaccttca gcgtccggac cgcgccatcc tgcgtgtacg ttacaacatg gccccgttct     1320

ctgttgaaga gactaagagc ccgggtccgg accgtcgtag cattgagatt tctaaagtaa     1380

ttaaaggtgc gctggaaccg gcacttattc tggaaatgtt cccgcgtact gctattgatg     1440

tattcatcga agttctgcaa gcggatgccg gtacgcgcgt tgcaggtatc accgcggcta     1500

gcctggcact ggccgacgct ggtattccga tgcgcgacct ggttgccgct tgtgcggcgg     1560

gtaaaatcga aggcgaaatt gttctggacc tgaacaaaga agaagataac tacggcgaag     1620

cggacgtgcc ggtagctatc atgccgctga agaacgatat taccctgctg caaatggatg     1680

gttacctgac caaggacgag ttcatcgagg cggttaaact ggctattaaa ggtgctaagg     1740

cggtgtatca gaaacagcgt gaagcactga aagaaaagta tctgaaaatt gcccaggaag     1800

ttgagggatc ctaaactagt gaattcgctt aagtcgaaca gaaagtaatc gtattgtaca     1860

cggccgcata atcgaaatta atacgactca ctatagggag accacaacgg tttcccatct     1920

tagtatatta gttaagtata agaaggagat ataccatggg tgtcgactct gataatgaaa     1980

tcgtggcggg tattatgcgc gaccatatta ttaacctgct taaggaaggt aaacgtattg     2040

acgaccgcgg tttcgaagat taccgcccga ttgaaattga ggtaggtgtt attgaaaaag     2100

cggagggtag cgcgctggtg aaactgggta gcactcaggt tctggtgggt attaaaacct     2160

ctctgggcga gccgtttccg gacaccccga acatgggcgt gatgaccacc aatgttgaac     2220

ttgttccgct ggcgtctccg actttcgaac cgggcccgcc ggacgagcgt gctatcgaac     2280

tggcacgtgt tattgaccgt ggtattcgtg agtccaaagc actgaatctg gaaaaaatgg     2340

ttatcgtgcc gggcaaaatt gttcgcgtag tttttatcga cgttcacgtt cttgaccacg     2400

acggtaacct gatggacgcc atcggtattg cagctatcgc cgcactgctg aacgcacgtg     2460

ttccgaaagt tcgctacaac gaagaaacgg gtgaagttga aaccctggat gaaactgaac     2520

cgctgccggt tgaaaagatt ccggttccgg ttactttcgc taaaattggt aatatcctgg     2580

tagttgaccc gagcctggat gaagagctcg tgatggacgg caaaattacc attaccactg     2640

acgaaaccgg ccatatttct gccgttcaga aatctgaagg tggcgctttc aaactggaag     2700

aagtaatgta cgcggtggaa actgcattca agaaggcgga agaaatccgt aaactgattc     2760

tggaagctgt tgagaaggcc aaacagctcg agtaagcggc cgcaagcttg atccggctgc     2820

taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata     2880

accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc     2940

cggatctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc     3000

ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt     3060

acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc     3120

ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct     3180

ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat     3240

ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc     3300

acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc     3360

tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg     3420

atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca     3480

cgtcgagtta tggacagcaa gcgaaccgga attgccagct ggggcgccct ctggtaaggt     3540

tgggaagccc tgcaaagtaa actggatggc tttcttgccg ccaaggatct gatggcgcag     3600

gggatcaaga tttgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg     3660

attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca     3720

acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt     3780

tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctccaagacg aggcagcgcg     3840

gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga     3900

agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca     3960

ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct     4020

tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac     4080

tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc     4140

gccagccgaa ctgttcgcca ggctcaaggc gcggatgccc gacggcgagg atctcgtcgt     4200

gacccacggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt     4260

catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg     4320

tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat     4380

cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc     4440

gggactctgg ggttcgaaat tctagtgcat tggtaactgt cagaccaagt ttactcatat     4500

atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt     4560

tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac     4620

cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc     4680

ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca     4740

actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta     4800

gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct     4860

ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg     4920

gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc     4980

acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta     5040

tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg     5100

gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt     5160

cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg     5220

cggagcctat ggaaaaacgc cagcagtcga gtttgtctcc gaccatcagg cacctgagtc     5280

gctgtctttt tcgtgacatt cagttcgctg cgctcacggc tctggcagtg aatgggggta     5340

aatggcacta caggcgcctt ttatggattc atgcaaggaa actacccata atacaagaaa     5400

agcccgtcac gggcttctca gggcgtttta tggcgggtct gctatgtggt gctatctgac     5460

tttttgctgt tcagcagttc ctgccctctg attttccagt ctgaccactt cggattatcc     5520

cgtctagtac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt     5580

ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga     5640

taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga     5700

gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcag           5754



<210>    SEQ ID No: 37
<211>    4795 
<212>    DNA
<213>    sequncia artificial

<220>
<223>    Vetor de expresso para fuso de antgeno com as protenas Rrp41 Rrp42 do exossomo de Pyrococcus abyssi

<400>    37

gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct       60

agaaataatt ttgtttaact ttaagaagga gatatacata tggctacgcg tgcagagaaa      120

ccggaaggtc tgaaactgat tgatgaaaac ggccgccgta ttgacggtcg taaaaaatac      180

gaactgcgtc cgattaaaat ggaagtgggt gtactgaaaa acgctaacgg ttccgcgtac      240

atcgaatggg gcaaaaacaa aattatcgca gcggtttacg gtccgcgtga actccacccg      300

aaacaccttc agcgtccgga ccgcgccatc ctgcgtgtac gttacaacat ggccccgttc      360

tctgttgaag agactaagag cccgggtccg gaccgtcgta gcattgagat ttctaaagta      420

attaaaggtg cgctggaacc ggcacttatt ctggaaatgt tcccgcgtac tgctattgat      480

gtattcatcg aagttctgca agcggatgcc ggtacgcgcg ttgcaggtat caccgcggct      540

agcctggcac tggccgacgc tggtattccg atgcgcgacc tggttgccgc ttgtgcggcg      600

ggtaaaatcg aaggcgaaat tgttctggac ctgaacaaag aagaagataa ctacggcgaa      660

gcggacgtgc cggtagctat catgccgctg aagaacgata ttaccctgct gcaaatggat      720

ggttacctga ccaaggacga gttcatcgag gcggttaaac tggctattaa aggtgctaag      780

gcggtgtatc agaaacagcg tgaagcactg aaagaaaagt atctgaaaat tgcccaggaa      840

gttgagggat cctaaactag tgaattcgct taagtcgaac agaaagtaat cgtattgtac      900

acggccgcat aatcgaaatt aatacgactc actataggga gaccacaacg gtttcccatc      960

ttagtatatt agttaagtat aagaaggaga tataccatgg gtgtcgactc tgataatgaa     1020

atcgtggcgg gtattatgcg cgaccatatt attaacctgc ttaaggaagg taaacgtatt     1080

gacgaccgcg gtttcgaaga ttaccgcccg attgaaattg aggtaggtgt tattgaaaaa     1140

gcggagggta gcgcgctggt gaaactgggt agcactcagg ttctggtggg tattaaaacc     1200

tctctgggcg agccgtttcc ggacaccccg aacatgggcg tgatgaccac caatgttgaa     1260

cttgttccgc tggcgtctcc gactttcgaa ccgggcccgc cggacgagcg tgctatcgaa     1320

ctggcacgtg ttattgaccg tggtattcgt gagtccaaag cactgaatct ggaaaaaatg     1380

gttatcgtgc cgggcaaaat tgttcgcgta gtttttatcg acgttcacgt tcttgaccac     1440

gacggtaacc tgatggacgc catcggtatt gcagctatcg ccgcactgct gaacgcacgt     1500

gttccgaaag ttcgctacaa cgaagaaacg ggtgaagttg aaaccctgga tgaaactgaa     1560

ccgctgccgg ttgaaaagat tccggttccg gttactttcg ctaaaattgg taatatcctg     1620

gtagttgacc cgagcctgga tgaagagctc gtgatggacg gcaaaattac cattaccact     1680

gacgaaaccg gccatatttc tgccgttcag aaatctgaag gtggcgcttt caaactggaa     1740

gaagtaatgt acgcggtgga aactgcattc aagaaggcgg aagaaatccg taaactgatt     1800

ctggaagctg ttgagaaggc caaacagctc gagtaagcgg ccgcaagctt gatccggctg     1860

ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat     1920

aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat     1980

ccggatctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag     2040

cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt     2100

tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt     2160

cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc     2220

tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga     2280

tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc     2340

cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt     2400

ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct     2460

gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc     2520

acgtcgagtt atggacagca agcgaaccgg aattgccagc tggggcgccc tctggtaagg     2580

ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc gccaaggatc tgatggcgca     2640

ggggatcaag atttgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg     2700

gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac     2760

aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg     2820

ttctttttgt caagaccgac ctgtccggtg ccctgaatga actccaagac gaggcagcgc     2880

ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg     2940

aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc     3000

accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc     3060

ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta     3120

ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg     3180

cgccagccga actgttcgcc aggctcaagg cgcggatgcc cgacggcgag gatctcgtcg     3240

tgacccacgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat     3300

tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc     3360

gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta     3420

tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag     3480

cgggactctg gggttcgaaa ttctagtgca ttggtaactg tcagaccaag tttactcata     3540

tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct     3600

ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga     3660

ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg     3720

cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc     3780

aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct     3840

agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc     3900

tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt     3960

ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg     4020

cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct     4080

atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag     4140

ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag     4200

tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg     4260

gcggagccta tggaaaaacg ccagcagtcg agtttgtctc cgaccatcag gcacctgagt     4320

cgctgtcttt ttcgtgacat tcagttcgct gcgctcacgg ctctggcagt gaatgggggt     4380

aaatggcact acaggcgcct tttatggatt catgcaagga aactacccat aatacaagaa     4440

aagcccgtca cgggcttctc agggcgtttt atggcgggtc tgctatgtgg tgctatctga     4500

ctttttgctg ttcagcagtt cctgccctct gattttccag tctgaccact tcggattatc     4560

ccgtctagta cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt     4620

tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg     4680

ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag     4740

agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcag          4795


<210>    SEQ ID No: 38
<211>    29 
<212>    DNA
<213>    sequncia artificial

<220>
<223>    Primer forward para amplificao por PCR do gene de Rrp42

<400>    38

accatatggg ttctgataat gaaatcgtg                                         29



<210>    SEQ ID No: 39
<211>    47 
<212>    DNA
<213>    sequncia artificial

<220>
<223>    Primer inverso para amplificao por PCR do gene de Rrp42

<400>    39

aactgcaggg atccctcgag accaccctgt ttggccttct caacagc                     47



<210>    SEQ ID No: 40
<211>    3917 
<212>    DNA
<213>    sequncia artificial

<220>
<223>    Vetor de expresso para protenas recombinantes de fuso em fuso com a Rrp42  do exossomo de P. abyssi

<400>    40

gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct       60

agaaataatt ttgtttaact ttaagaagga gatatacata tgggttctga taatgaaatc      120

gtggcgggta ttatgcgcga ccatattatt aacctgctta aggaaggtaa acgtattgac      180

gaccgcggtt tcgaagatta ccgcccgatt gaaattgagg taggtgttat tgaaaaagcg      240

gagggtagcg cgctggtgaa actgggtagc actcaggttc tggtgggtat taaaacctct      300

ctgggcgagc cgtttccgga caccccgaac atgggcgtga tgaccaccaa tgttgaactt      360

gttccgctgg cgtctccgac tttcgaaccg ggcccgccgg acgagcgtgc tatcgaactg      420

gcacgtgtta ttgaccgtgg tattcgtgag tccaaagcac tgaatctgga aaaaatggtt      480

atcgtgccgg gcaaaattgt tcgcgtagtt tttatcgacg ttcacgttct tgaccacgac      540

ggtaacctga tggacgccat cggtattgca gctatcgccg cactgctgaa cgcacgtgtt      600

ccgaaagttc gctacaacga agaaacgggt gaagttgaaa ccctggatga aactgaaccg      660

ctgccggttg aaaagattcc ggttccggtt actttcgcta aaattggtaa tatcctggta      720

gttgacccga gcctggatga agagctcgtg atggacggca aaattaccat taccactgac      780

gaaaccggcc atatttctgc cgttcagaaa tctgaaggtg gcgctttcaa actggaagaa      840

gtaatgtacg cggtggaaac tgcattcaag aaggcggaag aaatccgtaa actgattctg      900

gaagctgttg agaaggccaa acagggtggt ctcgagggat ccctgcagct ggtaccatgg      960

aattcgaagc ttgatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc     1020

accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt     1080

ttgctgaaag gaggaactat atccggatct ggcgtaatag cgaagaggcc cgcaccgatc     1140

gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat     1200

taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag     1260

cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc     1320

aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc     1380

ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt     1440

ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa     1500

caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg     1560

cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat     1620

taacgcttac aatttaggtg gcacgtcgag ttatggacag caagcgaacc ggaattgcca     1680

gctggggcgc cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttcttg     1740

ccgccaagga tctgatggcg caggggatca agatttgatc aagagacagg atgaggatcg     1800

tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg     1860

ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg     1920

ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat     1980

gaactccaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca     2040

gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg     2100

gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat     2160

gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa     2220

catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg     2280

gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcggatg     2340

cccgacggcg aggatctcgt cgtgacccac ggcgatgcct gcttgccgaa tatcatggtg     2400

gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat     2460

caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac     2520

cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc     2580

cttcttgacg agttcttctg agcgggactc tggggttcga aattctagtg cattggtaac     2640

tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta     2700

aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt     2760

tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt     2820

tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt     2880

gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc     2940

agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg     3000

tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg     3060

ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt     3120

cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac     3180

tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg     3240

acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg     3300

gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat     3360

ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcagt cgagtttgtc     3420

tccgaccatc aggcacctga gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac     3480

ggctctggca gtgaatgggg gtaaatggca ctacaggcgc cttttatgga ttcatgcaag     3540

gaaactaccc ataatacaag aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg     3600

tctgctatgt ggtgctatct gactttttgc tgttcagcag ttcctgccct ctgattttcc     3660

agtctgacca cttcggatta tcccgtctag tacgcggcct ttttacggtt cctggccttt     3720

tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt     3780

attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag     3840

tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg     3900

ccgattcatt aatgcag                                                    3917




<210>    41
<211>    368
<212>    DNA
<213>    Sequncia Artificial 

<220>
<223>    Sequncia artificial codificante dos dominios Z-Z da protena A de
       S. aureus

<220>
<221>    CDS
<222>    (3)..(368)
<223>    Domnios Z-Z da protena A de S. aureus

<400>    41

cc atg ggt gtg gat aac aaa ttt aac aaa gaa cag cag aat gcg ttt          47
   Met Gly Val Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe            
   1               5                   10                  15             

tac gaa att ctg cac ctg ccg aac ctt aac gaa gaa cag cgt aac gct         95
Tyr Glu Ile Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Ala           
                20                  25                  30                

ttc att cag tct ctg aaa gat gac cct tct cag tct gcg aac ctg ctg        143
Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu           
            35                  40                  45                    

gct gaa gct aaa aaa ctg aac gac gct cag gct cct aaa gtt gac aac        191
Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Val Asp Asn           
        50                  55                  60                        

aaa ttc aac aaa gaa cag cag aac gct ttc tac gaa atc ttg cat ctg        239
Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu           
    65                  70                  75                            

ccg aac ctg aac gaa gaa cag cgc aac gct ttc atc cag tcc ctg aaa        287
Pro Asn Leu Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys           
80                  85                  90                  95            

gac gac cct tcc cag tct gct aac ctg ctg gcg gaa gcg aaa aaa ctg        335
Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu           
                100                 105                 110               

aac gat gct cag gcg cct aaa gtg ggc gtc gac                            368
Asn Asp Ala Gln Ala Pro Lys Val Gly Val Asp                               
            115                 120                                       
<210>    42
<211>    122
<212>    Protena
<213>    Sequncia Artificial 

<220>
<223>    Constructo Sintpetico

<400>    42

Met Gly Val Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr 
1               5                   10                  15      


Glu Ile Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Ala Phe 
            20                  25                  30          


Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala 
        35                  40                  45              


Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Val Asp Asn Lys 
    50                  55                  60                  


Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro 
65                  70                  75                  80  


Asn Leu Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys Asp 
                85                  90                  95      


Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn 
            100                 105                 110         


Asp Ala Gln Ala Pro Lys Val Gly Val Asp 
        115                 120         

<210>    43
<211>    5148
<212>    DNA
<213>    Sequncia Artificial 

<220>
<223>    Vetor de expresso para fuso das protenas Rrp41 e ZZ-Rrp42 

<400>    43

gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct       60

agaataattt tgtttaactt taagaaggag atatacatat ggctacgcgt gcagagaaac      120

cggaaggtct gaaactgatt gatgaaaacg gccgccgtat tgacggtcgt aaaaaatacg      180

aactgcgtcc gattaaaatg gaagtgggtg tactgaaaaa cgctaacggt tccgcgtaca      240

tcgaatgggg caaaaacaaa attatcgcag cggtttacgg tccgcgtgaa ctccacccga      300

aacaccttca gcgtccggac cgcgccatcc tgcgtgtacg ttacaacatg gccccgttct      360

ctgttgaaga gactaagagc ccgggtccgg accgtcgtag cattgagatt tctaaagtaa      420

ttaaaggtgc gctggaaccg gcacttattc tggaaatgtt cccgcgtact gctattgatg      480

tattcatcga agttctgcaa gcggatgccg gtacgcgcgt tgcaggtatc accgcggcta      540

gcctggcact ggccgacgct ggtattccga tgcgcgacct ggttgccgct tgtgcggcgg      600

gtaaaatcga aggcgaaatt gttctggacc tgaacaaaga agaagataac tacggcgaag      660

cggacgtgcc ggtagctatc atgccgctga agaacgatat taccctgctg caaatggatg      720

gttacctgac caaggacgag ttcatcgagg cggttaaact ggctattaaa ggtgctaagg      780

cggtgtatca gaaacagcgt gaagcactga aagaaaagta tctgaaaatt gcccaggaag      840

ttgagggatc ctaaactagt gaattcgctt aagtcgaaca gaaagtaatc gtattgtaca      900

cggccgcata atcgaaatta atacgactca ctatagggag accacaacgg tttcccatct      960

tagtatatta gttaagtata agaaggagat ataccatggg tgtggataac aaatttaaca     1020

aagaacagca gaatgcgttt tacgaaattc tgcacctgcc gaaccttaac gaagaacagc     1080

gtaacgcttt cattcagtct ctgaaagatg acccttctca gtctgcgaac ctgctggctg     1140

aagctaaaaa actgaacgac gctcaggctc ctaaagttga caacaaattc aacaaagaac     1200

agcagaacgc tttctacgaa atcttgcatc tgccgaacct gaacgaagaa cagcgcaacg     1260

ctttcatcca gtccctgaaa gacgaccctt cccagtctgc taacctgctg gcggaagcga     1320

aaaaactgaa cgatgctcag gcgcctaaag tgggcgtcga ctctgataat gaaatcgtgg     1380

cgggtattat gcgcgaccat attattaacc tgcttaagga aggtaaacgt attgacgacc     1440

gcggtttcga agattaccgc ccgattgaaa ttgaggtagg tgttattgaa aaagcggagg     1500

gtagcgcgct ggtgaaactg ggtagcactc aggttctggt gggtattaaa acctctctgg     1560

gcgagccgtt tccggacacc ccgaacatgg gcgtgatgac caccaatgtt gaacttgttc     1620

cgctggcgtc tccgactttc gaaccgggcc cgccggacga gcgtgctatc gaactggcac     1680

gtgttattga ccgtggtatt cgtgagtcca aagcactgaa tctggaaaaa atggttatcg     1740

tgccgggcaa aattgttcgc gtagttttta tcgacgttca cgttcttgac cacgacggta     1800

acctgatgga cgccatcggt attgcagcta tcgccgcact gctgaacgca cgtgttccga     1860

aagttcgcta caacgaagaa acgggtgaag ttgaaaccct ggatgaaact gaaccgctgc     1920

cggttgaaaa gattccggtt ccggttactt tcgctaaaat tggtaatatc ctggtagttg     1980

acccgagcct ggatgaagag ctcgtgatgg acggcaaaat taccattacc actgacgaaa     2040

ccggccatat ttctgccgtt cagaaatctg aaggtggcgc tttcaaactg gaagaagtaa     2100

tgtacgcggt ggaaactgca ttcaagaagg cggaagaaat ccgtaaactg attctggaag     2160

ctgttgagaa ggccaaacag ctcgagtaag cggccgcaag cttgatccgg ctgctaacaa     2220

agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct     2280

tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatc     2340

tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat     2400

ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc     2460

agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc     2520

tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg     2580

ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca     2640

cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc     2700

tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct     2760

tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa     2820

caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacgtcga     2880

gttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa     2940

gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc     3000

aagatttgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca     3060

cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac     3120

aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt     3180

tgtcaagacc gacctgtccg gtgccctgaa tgaactccaa gacgaggcag cgcggctatc     3240

gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg     3300

aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc     3360

tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc     3420

ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat     3480

ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc     3540

cgaactgttc gccaggctca aggcgcggat gcccgacggc gaggatctcg tcgtgaccca     3600

cggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga     3660

ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat     3720

tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc     3780

tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact     3840

ctggggttcg aaattctagt gcattggtaa ctgtcagacc aagtttactc atatatactt     3900

tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat     3960

aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta     4020

gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa     4080

acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt     4140

tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag     4200

ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta     4260

atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca     4320

agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag     4380

cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa     4440

agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga     4500

acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc     4560

gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc     4620

ctatggaaaa acgccagcag tcgagtttgt ctccgaccat caggcacctg agtcgctgtc     4680

tttttcgtga cattcagttc gctgcgctca cggctctggc agtgaatggg ggtaaatggc     4740

actacaggcg ccttttatgg attcatgcaa ggaaactacc cataatacaa gaaaagcccg     4800

tcacgggctt ctcagggcgt tttatggcgg gtctgctatg tggtgctatc tgactttttg     4860

ctgttcagca gttcctgccc tctgattttc cagtctgacc acttcggatt atcccgtcta     4920

gtacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc     4980

tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc     5040

tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc     5100

aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcag                  5148




<210>    44
<211>    405
<212>    Protena
<213>    Sequncia Artificial 

<220>
<223>    Constructo Sinttico

<400>    42

Leu Ser Ile Arg Arg Arg Tyr Thr Met Gly Val Asp Asn Lys Phe Asn 
1               5                   10                  15      


Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu 
            20                  25                  30          


Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro 
        35                  40                  45              


Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala 
    50                  55                  60                  


Gln Ala Pro Lys Val Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala 
65                  70                  75                  80  


Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn 
                85                  90                  95      


Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu 
            100                 105                 110         


Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Val Gly 
        115                 120                 125             


Val Asp Ser Asp Asn Glu Ile Val Ala Gly Ile Met Arg Asp His Ile 
    130                 135                 140                 


Ile Asn Leu Leu Lys Glu Gly Lys Arg Ile Asp Asp Arg Gly Phe Glu 
145                 150                 155                 160 


Asp Tyr Arg Pro Ile Glu Ile Glu Val Gly Val Ile Glu Lys Ala Glu 
                165                 170                 175     


Gly Ser Ala Leu Val Lys Leu Gly Ser Thr Gln Val Leu Val Gly Ile 
            180                 185                 190         


Lys Thr Ser Leu Gly Glu Pro Phe Pro Asp Thr Pro Asn Met Gly Val 
        195                 200                 205             


Met Thr Thr Asn Val Glu Leu Val Pro Leu Ala Ser Pro Thr Phe Glu 
    210                 215                 220                 


Pro Gly Pro Pro Asp Glu Arg Ala Ile Glu Leu Ala Arg Val Ile Asp 
225                 230                 235                 240 


Arg Gly Ile Arg Glu Ser Lys Ala Leu Asn Leu Glu Lys Met Val Ile 
                245                 250                 255     


Val Pro Gly Lys Ile Val Arg Val Val Phe Ile Asp Val His Val Leu 
            260                 265                 270         


Asp His Asp Gly Asn Leu Met Asp Ala Ile Gly Ile Ala Ala Ile Ala 
        275                 280                 285             


Ala Leu Leu Asn Ala Arg Val Pro Lys Val Arg Tyr Asn Glu Glu Thr 
    290                 295                 300                 


Gly Glu Val Glu Thr Leu Asp Glu Thr Glu Pro Leu Pro Val Glu Lys 
305                 310                 315                 320 


Ile Pro Val Pro Val Thr Phe Ala Lys Ile Gly Asn Ile Leu Val Val 
                325                 330                 335     


Asp Pro Ser Leu Asp Glu Glu Leu Val Met Asp Gly Lys Ile Thr Ile 
            340                 345                 350         


Thr Thr Asp Glu Thr Gly His Ile Ser Ala Val Gln Lys Ser Glu Gly 
        355                 360                 365             


Gly Ala Phe Lys Leu Glu Glu Val Met Tyr Ala Val Glu Thr Ala Phe 
    370                 375                 380                 


Lys Lys Ala Glu Glu Ile Arg Lys Leu Ile Leu Glu Ala Val Glu Lys 
385                 390                 395                 400 


Ala Lys Gln Leu Glu 
                405




<210>    45
<211>    5148
<212>    DNA
<213>    Sequncia Artificial 

<220>
<223>    Vetor de expresso para fuso das protenas recombinantes com Rrp41 
       e ZZ-Rrp42 do exossomo de P. abyssi 


<220>
<221>    CDS
<222>    (966)..(2189)
<223>    Protena Z-Z Rrp42 

<400>    41

gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct       60

agaataattt tgtttaactt taagaaggag atatacatat ggctacgcgt gcagagaaac      120

cggaaggtct gaaactgatt gatgaaaacg gccgccgtat tgacggtcgt aaaaaatacg      180

aactgcgtcc gattaaaatg gaagtgggtg tactgaaaaa cgctaacggt tccgcgtaca      240

tcgaatgggg caaaaacaaa attatcgcag cggtttacgg tccgcgtgaa ctccacccga      300

aacaccttca gcgtccggac cgcgccatcc tgcgtgtacg ttacaacatg gccccgttct      360

ctgttgaaga gactaagagc ccgggtccgg accgtcgtag cattgagatt tctaaagtaa      420

ttaaaggtgc gctggaaccg gcacttattc tggaaatgtt cccgcgtact gctattgatg      480

tattcatcga agttctgcaa gcggatgccg gtacgcgcgt tgcaggtatc accgcggcta      540

gcctggcact ggccgacgct ggtattccga tgcgcgacct ggttgccgct tgtgcggcgg      600

gtaaaatcga aggcgaaatt gttctggacc tgaacaaaga agaagataac tacggcgaag      660

cggacgtgcc ggtagctatc atgccgctga agaacgatat taccctgctg caaatggatg      720

gttacctgac caaggacgag ttcatcgagg cggttaaact ggctattaaa ggtgctaagg      780

cggtgtatca gaaacagcgt gaagcactga aagaaaagta tctgaaaatt gcccaggaag      840

ttgagggatc ctaaactagt gaattcgctt aagtcgaaca gaaagtaatc gtattgtaca      900

cggccgcata atcgaaatta atacgactca ctatagggag accacaacgg tttcccatct      960

tagta tat tag tta agt ata aga agg aga tat acc atg ggt gtg gat aac     1010
      Tyr     Leu Ser Ile Arg Arg Arg Tyr Thr Met Gly Val Asp Asn         
      1                   5                   10                          

aaa ttt aac aaa gaa cag cag aat gcg ttt tac gaa att ctg cac ctg       1058
Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu           
15                  20                  25                  30            

ccg aac ctt aac gaa gaa cag cgt aac gct ttc att cag tct ctg aaa       1106
Pro Asn Leu Asn Glu Glu Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys           
                35                  40                  45                

gat gac cct tct cag tct gcg aac ctg ctg gct gaa gct aaa aaa ctg       1154
Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu           
            50                  55                  60                    

aac gac gct cag gct cct aaa gtt gac aac aaa ttc aac aaa gaa cag       1202
Asn Asp Ala Gln Ala Pro Lys Val Asp Asn Lys Phe Asn Lys Glu Gln           
        65                  70                  75                        

cag aac gct ttc tac gaa atc ttg cat ctg ccg aac ctg aac gaa gaa       1250
Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu Asn Glu Glu           
    80                  85                  90                            

cag cgc aac gct ttc atc cag tcc ctg aaa gac gac cct tcc cag tct       1298
Gln Arg Asn Ala Phe Ile Gln Ser Leu Lys Asp Asp Pro Ser Gln Ser           
95                  100                 105                 110           

gct aac ctg ctg gcg gaa gcg aaa aaa ctg aac gat gct cag gcg cct       1346
Ala Asn Leu Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala Gln Ala Pro           
                115                 120                 125               

aaa gtg ggc gtc gac tct gat aat gaa atc gtg gcg ggt att atg cgc       1394
Lys Val Gly Val Asp Ser Asp Asn Glu Ile Val Ala Gly Ile Met Arg           
            130                 135                 140                   

gac cat att att aac ctg ctt aag gaa ggt aaa cgt att gac gac cgc       1442
Asp His Ile Ile Asn Leu Leu Lys Glu Gly Lys Arg Ile Asp Asp Arg           
        145                 150                 155                       

ggt ttc gaa gat tac cgc ccg att gaa att gag gta ggt gtt att gaa       1490
Gly Phe Glu Asp Tyr Arg Pro Ile Glu Ile Glu Val Gly Val Ile Glu           
    160                 165                 170                           

aaa gcg gag ggt agc gcg ctg gtg aaa ctg ggt agc act cag gtt ctg       1538
Lys Ala Glu Gly Ser Ala Leu Val Lys Leu Gly Ser Thr Gln Val Leu           
175                 180                 185                 190           

gtg ggt att aaa acc tct ctg ggc gag ccg ttt ccg gac acc ccg aac       1586
Val Gly Ile Lys Thr Ser Leu Gly Glu Pro Phe Pro Asp Thr Pro Asn           
                195                 200                 205               

atg ggc gtg atg acc acc aat gtt gaa ctt gtt ccg ctg gcg tct ccg       1634
Met Gly Val Met Thr Thr Asn Val Glu Leu Val Pro Leu Ala Ser Pro           
            210                 215                 220                   

act ttc gaa ccg ggc ccg ccg gac gag cgt gct atc gaa ctg gca cgt       1682
Thr Phe Glu Pro Gly Pro Pro Asp Glu Arg Ala Ile Glu Leu Ala Arg           
        225                 230                 235                       

gtt att gac cgt ggt att cgt gag tcc aaa gca ctg aat ctg gaa aaa       1730
Val Ile Asp Arg Gly Ile Arg Glu Ser Lys Ala Leu Asn Leu Glu Lys           
    240                 245                 250                           

atg gtt atc gtg ccg ggc aaa att gtt cgc gta gtt ttt atc gac gtt       1778
Met Val Ile Val Pro Gly Lys Ile Val Arg Val Val Phe Ile Asp Val           
255                 260                 265                 270           

cac gtt ctt gac cac gac ggt aac ctg atg gac gcc atc ggt att gca       1826
His Val Leu Asp His Asp Gly Asn Leu Met Asp Ala Ile Gly Ile Ala           
                275                 280                 285               

gct atc gcc gca ctg ctg aac gca cgt gtt ccg aaa gtt cgc tac aac       1874
Ala Ile Ala Ala Leu Leu Asn Ala Arg Val Pro Lys Val Arg Tyr Asn           
            290                 295                 300                   

gaa gaa acg ggt gaa gtt gaa acc ctg gat gaa act gaa ccg ctg ccg       1922
Glu Glu Thr Gly Glu Val Glu Thr Leu Asp Glu Thr Glu Pro Leu Pro           
        305                 310                 315                       

gtt gaa aag att ccg gtt ccg gtt act ttc gct aaa att ggt aat atc       1970
Val Glu Lys Ile Pro Val Pro Val Thr Phe Ala Lys Ile Gly Asn Ile           
    320                 325                 330                           

ctg gta gtt gac ccg agc ctg gat gaa gag ctc gtg atg gac ggc aaa       2018
Leu Val Val Asp Pro Ser Leu Asp Glu Glu Leu Val Met Asp Gly Lys           
335                 340                 345                 350           

att acc att acc act gac gaa acc ggc cat att tct gcc gtt cag aaa       2066
Ile Thr Ile Thr Thr Asp Glu Thr Gly His Ile Ser Ala Val Gln Lys           
                355                 360                 365               

tct gaa ggt ggc gct ttc aaa ctg gaa gaa gta atg tac gcg gtg gaa       2114
Ser Glu Gly Gly Ala Phe Lys Leu Glu Glu Val Met Tyr Ala Val Glu           
            370                 375                 380                   

act gca ttc aag aag gcg gaa gaa atc cgt aaa ctg att ctg gaa gct       2162
Thr Ala Phe Lys Lys Ala Glu Glu Ile Arg Lys Leu Ile Leu Glu Ala           
        385                 390                 395                       

gtt gag aag gcc aaa cag ctc gag taa gcggccgcaa gcttgatccg             2209
Val Glu Lys Ala Lys Gln Leu Glu                                           
    400                 405                                               

gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta     2269

gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact     2329

atatccggat ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc     2389

gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg     2449

tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt     2509

tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc     2569

tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg     2629

gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg     2689

agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct     2749

cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg     2809

agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg     2869

tggcacgtcg agttatggac agcaagcgaa ccggaattgc cagctggggc gccctctggt     2929

aaggttggga agccctgcaa agtaaactgg atggctttct tgccgccaag gatctgatgg     2989

cgcaggggat caagatttga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa     3049

gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg     3109

gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc     3169

ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactcca agacgaggca     3229

gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc     3289

actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca     3349

tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat     3409

acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca     3469

cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg     3529

ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgga tgcccgacgg cgaggatctc     3589

gtcgtgaccc acggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct     3649

ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct     3709

acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac     3769

ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc     3829

tgagcgggac tctggggttc gaaattctag tgcattggta actgtcagac caagtttact     3889

catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga     3949

tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt     4009

cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct     4069

gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc     4129

taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc     4189

ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc     4249

tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg     4309

ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt     4369

cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg     4429

agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg     4489

gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt     4549

atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag     4609

gggggcggag cctatggaaa aacgccagca gtcgagtttg tctccgacca tcaggcacct     4669

gagtcgctgt ctttttcgtg acattcagtt cgctgcgctc acggctctgg cagtgaatgg     4729

gggtaaatgg cactacaggc gccttttatg gattcatgca aggaaactac ccataataca     4789

agaaaagccc gtcacgggct tctcagggcg ttttatggcg ggtctgctat gtggtgctat     4849

ctgacttttt gctgttcagc agttcctgcc ctctgatttt ccagtctgac cacttcggat     4909

tatcccgtct agtacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac     4969

atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga     5029

gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg     5089

gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcag      5148

