                         SEQUENCE LISTING

<110>  Regeneron Pharmaceuticals, Inc.
       Yale University
       Institute for Research in Biomedicine (IRB)
       Murphy, Andrew J
       Stevens, Sean
       Flavell, Richard
       Manz, Markus Gabriel
       Shan, Liang
 
<120>  GENETICALLY MODIFIED NON-HUMAN ANIMALS EXPRESSING HUMAN EPO

<130>  REGN-013WO

<150>  US62/000,460
<151>  2014-05-19

<160>  19    

<170>  PatentIn version 3.5

<210>  1
<211>  715
<212>  DNA
<213>  Mus musculus

<400>  1
gatgaagact tgcagcgtgg acactggccc agccccgggt cgctaaggag ctccggcagc       60

taggcgcgga gatgggggtg cccgaacgtc ccaccctgct gcttttactc tccttgctac      120

tgattcctct gggcctccca gtcctctgtg ctcccccacg cctcatctgc gacagtcgag      180

ttctggagag gtacatctta gaggccaagg aggcagaaaa tgtcacgatg ggttgtgcag      240

aaggtcccag actgagtgaa aatattacag tcccagatac caaagtcaac ttctatgctt      300

ggaaaagaat ggaggtggaa gaacaggcca tagaagtttg gcaaggcctg tccctgctct      360

cagaagccat cctgcaggcc caggccctgc tagccaattc ctcccagcca ccagagaccc      420

ttcagcttca tatagacaaa gccatcagtg gtctacgtag cctcacttca ctgcttcggg      480

tactgggagc tcagaaggaa ttgatgtcgc ctccagatac caccccacct gctccactcc      540

gaacactcac agtggatact ttctgcaagc tcttccgggt ctacgccaac ttcctccggg      600

ggaaactgaa gctgtacacg ggagaggtct gcaggagagg ggacaggtga catgctgctg      660

ccaccgtggt ggaccgacga acttgctccc cgtcactgtg tcatgccaac cctcc           715


<210>  2
<211>  192
<212>  PRT
<213>  Mus musculus

<400>  2

Met Gly Val Pro Glu Arg Pro Thr Leu Leu Leu Leu Leu Ser Leu Leu 
1               5                   10                  15      


Leu Ile Pro Leu Gly Leu Pro Val Leu Cys Ala Pro Pro Arg Leu Ile 
            20                  25                  30          


Cys Asp Ser Arg Val Leu Glu Arg Tyr Ile Leu Glu Ala Lys Glu Ala 
        35                  40                  45              


Glu Asn Val Thr Met Gly Cys Ala Glu Gly Pro Arg Leu Ser Glu Asn 
    50                  55                  60                  


Ile Thr Val Pro Asp Thr Lys Val Asn Phe Tyr Ala Trp Lys Arg Met 
65                  70                  75                  80  


Glu Val Glu Glu Gln Ala Ile Glu Val Trp Gln Gly Leu Ser Leu Leu 
                85                  90                  95      


Ser Glu Ala Ile Leu Gln Ala Gln Ala Leu Leu Ala Asn Ser Ser Gln 
            100                 105                 110         


Pro Pro Glu Thr Leu Gln Leu His Ile Asp Lys Ala Ile Ser Gly Leu 
        115                 120                 125             


Arg Ser Leu Thr Ser Leu Leu Arg Val Leu Gly Ala Gln Lys Glu Leu 
    130                 135                 140                 


Met Ser Pro Pro Asp Thr Thr Pro Pro Ala Pro Leu Arg Thr Leu Thr 
145                 150                 155                 160 


Val Asp Thr Phe Cys Lys Leu Phe Arg Val Tyr Ala Asn Phe Leu Arg 
                165                 170                 175     


Gly Lys Leu Lys Leu Tyr Thr Gly Glu Val Cys Arg Arg Gly Asp Arg 
            180                 185                 190         


<210>  3
<211>  1340
<212>  DNA
<213>  homo sapiens

<400>  3
cccggagccg gaccggggcc accgcgcccg ctctgctccg acaccgcgcc ccctggacag       60

ccgccctctc ctccaggccc gtggggctgg ccctgcaccg ccgagcttcc cgggatgagg      120

gcccccggtg tggtcacccg gcgcgcccca ggtcgctgag ggaccccggc caggcgcgga      180

gatgggggtg cacgaatgtc ctgcctggct gtggcttctc ctgtccctgc tgtcgctccc      240

tctgggcctc ccagtcctgg gcgccccacc acgcctcatc tgtgacagcc gagtcctgga      300

gaggtacctc ttggaggcca aggaggccga gaatatcacg acgggctgtg ctgaacactg      360

cagcttgaat gagaatatca ctgtcccaga caccaaagtt aatttctatg cctggaagag      420

gatggaggtc gggcagcagg ccgtagaagt ctggcagggc ctggccctgc tgtcggaagc      480

tgtcctgcgg ggccaggccc tgttggtcaa ctcttcccag ccgtgggagc ccctgcagct      540

gcatgtggat aaagccgtca gtggccttcg cagcctcacc actctgcttc gggctctggg      600

agcccagaag gaagccatct cccctccaga tgcggcctca gctgctccac tccgaacaat      660

cactgctgac actttccgca aactcttccg agtctactcc aatttcctcc ggggaaagct      720

gaagctgtac acaggggagg cctgcaggac aggggacaga tgaccaggtg tgtccacctg      780

ggcatatcca ccacctccct caccaacatt gcttgtgcca caccctcccc cgccactcct      840

gaaccccgtc gaggggctct cagctcagcg ccagcctgtc ccatggacac tccagtgcca      900

gcaatgacat ctcaggggcc agaggaactg tccagagagc aactctgaga tctaaggatg      960

tcacagggcc aacttgaggg cccagagcag gaagcattca gagagcagct ttaaactcag     1020

ggacagagcc atgctgggaa gacgcctgag ctcactcggc accctgcaaa atttgatgcc     1080

aggacacgct ttggaggcga tttacctgtt ttcgcaccta ccatcaggga caggatgacc     1140

tggagaactt aggtggcaag ctgtgacttc tccaggtctc acgggcatgg gcactccctt     1200

ggtggcaaga gcccccttga caccggggtg gtgggaacca tgaagacagg atgggggctg     1260

gcctctggct ctcatggggt ccaagttttg tgtattcttc aacctcattg acaagaactg     1320

aaaccaccaa aaaaaaaaaa                                                 1340


<210>  4
<211>  193
<212>  PRT
<213>  homo sapiens

<400>  4

Met Gly Val His Glu Cys Pro Ala Trp Leu Trp Leu Leu Leu Ser Leu 
1               5                   10                  15      


Leu Ser Leu Pro Leu Gly Leu Pro Val Leu Gly Ala Pro Pro Arg Leu 
            20                  25                  30          


Ile Cys Asp Ser Arg Val Leu Glu Arg Tyr Leu Leu Glu Ala Lys Glu 
        35                  40                  45              


Ala Glu Asn Ile Thr Thr Gly Cys Ala Glu His Cys Ser Leu Asn Glu 
    50                  55                  60                  


Asn Ile Thr Val Pro Asp Thr Lys Val Asn Phe Tyr Ala Trp Lys Arg 
65                  70                  75                  80  


Met Glu Val Gly Gln Gln Ala Val Glu Val Trp Gln Gly Leu Ala Leu 
                85                  90                  95      


Leu Ser Glu Ala Val Leu Arg Gly Gln Ala Leu Leu Val Asn Ser Ser 
            100                 105                 110         


Gln Pro Trp Glu Pro Leu Gln Leu His Val Asp Lys Ala Val Ser Gly 
        115                 120                 125             


Leu Arg Ser Leu Thr Thr Leu Leu Arg Ala Leu Gly Ala Gln Lys Glu 
    130                 135                 140                 


Ala Ile Ser Pro Pro Asp Ala Ala Ser Ala Ala Pro Leu Arg Thr Ile 
145                 150                 155                 160 


Thr Ala Asp Thr Phe Arg Lys Leu Phe Arg Val Tyr Ser Asn Phe Leu 
                165                 170                 175     


Arg Gly Lys Leu Lys Leu Tyr Thr Gly Glu Ala Cys Arg Thr Gly Asp 
            180                 185                 190         


Arg 
    


<210>  5
<211>  226
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide sequence


<220>
<221>  misc_feature
<222>  (1)..(115)
<223>  mouse sequence

<220>
<221>  misc_feature
<222>  (116)..(128)
<223>  human Exon 1

<220>
<221>  misc_feature
<222>  (116)..(226)
<223>  human sequence

<400>  5
tcttccaggc tagtggggtg atctggccct acagaacttc caaggatgaa gacttgcagc       60

gtggacactg gcccagcccc gggtcgctaa ggagctccgg cagctaggcg cggagatggg      120

ggtgcacggt gagtactcgc gggctgggcg ctcccgcccg cccgggtccc tgtttgagcg      180

gggatttagc gccccggcta ttggccagga ggtggctggg ttcaag                     226


<210>  6
<211>  1054
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic polynucleotide sequence


<220>
<221>  misc_feature
<222>  (1)..(115)
<223>  human Exon 5

<220>
<221>  misc_feature
<222>  (1)..(926)
<223>  human sequence

<220>
<221>  misc_feature
<222>  (927)..(1054)
<223>  5' end of selection cassette

<400>  6
actccgaaca atcactgctg acactttccg caaactcttc cgagtctact ccaatttcct       60

ccggggaaag ctgaagctgt acacagggga ggcctgcagg acaggggaca gatgaccagg      120

tgtgtccacc tgggcatatc caccacctcc ctcaccaaca ttgcttgtgc cacaccctcc      180

cccgccactc ctgaaccccg tcgaggggct ctcagctcag cgccagcctg tcccatggac      240

actccagtgc cagcaatgac atctcagggg ccagaggaac tgtccagaga gcaactctga      300

gatctaagga tgtcacaggg ccaacttgag ggcccagagc aggaagcatt cagagagcag      360

ctttaaactc agggacagag ccatgctggg aagacgcctg agctcactcg gcaccctgca      420

aaatttgatg ccaggacacg ctttggaggc gatttacctg ttttcgcacc taccatcagg      480

gacaggatga cctggagaac ttaggtggca agctgtgact tctccaggtc tcacgggcat      540

gggcactccc ttggtggcaa gagccccctt gacaccgggg tggtgggaac catgaagaca      600

ggatgggggc tggcctctgg ctctcatggg gtccaagttt tgtgtattct tcaacctcat      660

tgacaagaac tgaaaccacc aatatgactc ttggcttttc tgttttctgg gaacctccaa      720

atcccctggc tctgtcccac tcctggcagc agtgcagcag gtccaggtcc gggaaacgag      780

gggtggaggg ggctgggccc tacgtgctgt ctcacacagc ctgtctgacc tctcgaccct      840

accgggcctg aggccacaag ctctgcctac gctggtcaat aaggtgtctc cattcaaggc      900

ctcaccgcag taaggcagct gccaacctcg agataacttc gtataatgta tgctatacga      960

agttatatgc atggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca     1020

cggcgagcgc tgccacgtca gacgaagggc gcag                                 1054


<210>  7
<211>  243
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic polynucleotide sequence


<220>
<221>  misc_feature
<222>  (1)..(121)
<223>  3' end of selection cassette

<220>
<221>  misc_feature
<222>  (122)..(243)
<223>  mouse sequence

<400>  7
gcctctgttc cacatacact tcattctcag tattgttttg ccaagttcta attccatcag       60

acctcgacct gcagccccta gataacttcg tataatgtat gctatacgaa gttatgctag      120

cgccaacccg ctaggacaag tgctgagtga gctggggcca ccgtttgagg aaacaggagc      180

cagtacagag gggttcccct ttaggggttg gtggcaatgg gcgaccctgg ttaatggatc      240

att                                                                    243


<210>  8
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic polynucleotide sequence

<400>  8
catctgcgac agtcgagttc                                                   20


<210>  9
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic polynucleotide sequence

<400>  9
ccagggagct taccgtgac                                                    19


<210>  10
<211>  27
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic polynucleotide sequence

<400>  10
aggtacatct tagaggccaa ggaggca                                           27


<210>  11
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic polynucleotide sequence

<400>  11
acagccgagt cctggagag                                                    19


<210>  12
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic polynucleotide sequence

<400>  12
aagccctgag cgtgagttc                                                    19


<210>  13
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic polynucleotide sequence

<400>  13
aggccaagga ggccgagaat atcacg                                            26


<210>  14
<211>  19
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic polynucleotide sequence

<400>  14
gagccctgca ctggacaac                                                    19


<210>  15
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic polynucleotide sequence

<400>  15
tcccatgaac gctgagagtc                                                   20


<210>  16
<211>  28
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic polynucleotide sequence

<400>  16
agggtcaagg agccatagac agaatggc                                          28


<210>  17
<211>  200
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic polynucleotide sequence


<220>
<221>  misc_feature
<222>  (1)..(100)
<223>  mouse sequence

<220>
<221>  misc_feature
<222>  (101)..(200)
<223>  human sequence

<400>  17
agctctccta ccactagact gctgagaccc gctgctctgc tcaggactcg atttccagta       60

cacaatctcc ctctttgaaa agtaccacac atcctggggt gctcttgcat ttgtgtgaca      120

ctttgctagc caggctcagt cctgggttcc aggtggggac tcaaacacac tggcacgagt      180

ctacattgga tattcttggt                                                  200


<210>  18
<211>  199
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic polynucleotide sequence


<220>
<221>  misc_feature
<222>  (1)..(100)
<223>  5' end of neomycin cassette

<220>
<221>  misc_feature
<222>  (101)..(199)
<223>  human sequence

<220>
<221>  misc_feature
<222>  (106)..(139)
<223>  LoxP

<400>  18
gctccccatt cctcactggc ccagcccctc ttccctactc tttctagccc ctgcctcatc       60

tccctggctg ccattgggag cctgccccac tggaagccag tcgagataac ttcgtataat      120

gtatgctata cgaagttata tgcatggcct ccgcgccggg ttttggcgcc tcccgcgggc      180

gcccccctcc tcacggcga                                                   199


<210>  19
<211>  200
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthetic polynucleotide sequence


<220>
<221>  misc_feature
<222>  (1)..(100)
<223>  3' neomycin cassette

<220>
<221>  misc_feature
<222>  (101)..(200)
<223>  mouse Exon 4

<400>  19
cattctcagt attgttttgc caagttctaa ttccatcaga cctcgacctg cagcccctag       60

ataacttcgt ataatgtatg ctatacgaag ttatgctagc tgtctcatag aggctggcga      120

tctggctcag ggacagccag tactgcaaag agtatccttg ttcatacctt ctcctagtgg      180

ccatctccct gggacagtca                                                  200


