                         SEQUENCE LISTING

<110>  BAVARIAN NORDIC, INC
 
<120>  COMBINATION THERAPY FOR TREATING CANCER WITH A RECOMBINANT 
       POXVIRUS EXPRESSING A TUMOR ANTIGEN AND AN ANTAGONIST OF TIM-3

<130>  BNIT0009PCT

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  2528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of a construct encoding a modified HER2 
       protein including two epitopes derived from tetanus toxin

<400>  1
agtatgcatt tttacggatg gagtctcggt ctaaaaacgg gaatgtacta tctacgtacg       60

aaacccgcat ccgctcccat tcaattcaca ttggacaagg ataaaataaa accactggtg      120

gtttgcgatt ccgaaatctg tacatcatgc agtggttaaa caaatctaga actagtttaa      180

ttaaggagct gttttgaata aaattttttt ataataaatc tagaactagt ggatcccccg      240

ggctgcagga attcgatcta gccgccacca tggagctggc ggccttgtgc cgctgggggc      300

tcctcctcgc cctcttgccc cccggagccg cgagcaccca agtgtgcacc ggcacagaca      360

tgaagctgcg gctccctgcc agtcccgaga cccacctgga catgctccgc cacctctacc      420

agggctgcca ggtggtgcag ggaaacctgg aactcaccta cctgcccacc aatgccagct      480

taagtttcct gcaggatatc caggaggtgc agggctacgt gctcatcgct cacaaccaag      540

tgaggcaggt cccactgcag aggctgcgga ttgtgcgagg cacccagctc tttgaggaca      600

actatgccct ggccgtgcta gacaatggag acccgctgaa caataccacc cctgtcacag      660

gggcctcccc aggaggcctg cgggagctgc agcttcgaag cctcacagag atcttgaaag      720

gaggggtctt gatccagcgg aacccccagc tctgctacca ggacacgatt ttgtggaagg      780

acatcttcca caagaacaac cagctggctc tcacactgat agacaccaac cgctctcggg      840

cctgccaccc ctgttctccg atgtgtaagg gctcccgctg ctggggagag agttctgagg      900

attgtcagag cctgacgcgc actgtctgtg ccggtggctg tgcccgctgc aaggggccac      960

tgcccactga ctgctgccat gagcagtgtg ctgccggctg cacgggcccc aagcactctg     1020

actgcctggc ctgcctccac ttcaaccaca gtggcatctg tgagctgcac tgcccagccc     1080

tggtccagta catcaaagct aactccaaat tcatcggtat caccgagctg cggtatacat     1140

tcggcgccag ctgtgtgact gcctgtccct acaactacct ttctacggac gtgggatcct     1200

gcaccctcgt ctgccccctg cacaaccaag aggtgacagc agaggatgga acacagcggt     1260

gtgagaagtg cagcaagccc tgtgcccgag tgtgctatgg tctgggcatg gagcacttgc     1320

gagaggtgag ggcagttacc agtgccaata tccaggagtt tgctggctgc aagaagatct     1380

ttgggagcct ggcatttctg ccggagagct ttgatgggga cccagcctcc aacactgccc     1440

cgctccagcc agagcagctc caagtgtttg agactctgga agagatcaca ggttacctat     1500

acatctcagc atggccggac agcctgcctg acctcagcgt cttccagaac ctgcaagtaa     1560

tccggggacg aattctgcac aatggcgcct actcgctgac cctgcaaggg ctgggcatca     1620

gctggctggg gctgcgctca ctgagggaac tgggcagtgg actggccctc atccaccata     1680

acacccacct ctgcttcgtg cacacggtgc cctgggacca gctctttcgg aacccgcacc     1740

aagctctgct ccacactgcc aaccggccag aggacgagtg tgtgggcgag ggcctggcct     1800

gccaccagct gtgcgcccga gggcactgct ggggtccagg gcccacccag tgtgtcaact     1860

gcagccagtt ccttcggggc caggagtgcg tggaggaatg ccgagtactg caggggctcc     1920

ccagggagta tgtgaatgcc aggcactgtt tgccgtgcca ccctgagtgt cagccccaga     1980

atggctcagt gacctgtttt ggaccggagg ctgaccagtg tgtggcctgt gcccactata     2040

aggaccctcc cttctgcgtg gcccgctgcc ccagcggtgt gaaacctgac ctctcctaca     2100

tgcccatctg gaagtttcca gatgaggagg gcgcatgcca gccttgcccc atcaactgca     2160

cccactcctg tgtggacctg gatgacaagg gctgccccgc cgagcagaga gccagccctc     2220

tgacgtcctt caacaacttc accgtgagct tctggctgcg cgtgcccaag gtgagcgcca     2280

gccacctgga gatcgtctct gcggtggttg gcattctgta gaagcttggt accgagctcg     2340

gatccactag tccagtgtgg tggaattctg cagatatcca gcacagtggc ggccatcaag     2400

cttatcgata ccgtcgacct cgaggggggg cccggtaccc agttaattaa ggatcccccg     2460

ggctgcagga attccatttt tattctcaaa tgagataaag tgaaaatata tatcatatat     2520

acaaagta                                                              2528


<210>  2
<211>  683
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of the Modified HER2 protein encoded by SEQ 
       ID NO:1


<220>
<221>  P2
<222>  (273)..(287)
<223>  T-helper cell epitope P2 derived from Tetanus Toxin

<220>
<221>  P30
<222>  (654)..(674)
<223>  T-helper cell epitope P30 derived from Tetanus Toxin

<400>  2

Met Glu Leu Ala Ala Leu Cys Arg Trp Gly Leu Leu Leu Ala Leu Leu 
1               5                   10                  15      


Pro Pro Gly Ala Ala Ser Thr Gln Val Cys Thr Gly Thr Asp Met Lys 
            20                  25                  30          


Leu Arg Leu Pro Ala Ser Pro Glu Thr His Leu Asp Met Leu Arg His 
        35                  40                  45              


Leu Tyr Gln Gly Cys Gln Val Val Gln Gly Asn Leu Glu Leu Thr Tyr 
    50                  55                  60                  


Leu Pro Thr Asn Ala Ser Leu Ser Phe Leu Gln Asp Ile Gln Glu Val 
65                  70                  75                  80  


Gln Gly Tyr Val Leu Ile Ala His Asn Gln Val Arg Gln Val Pro Leu 
                85                  90                  95      


Gln Arg Leu Arg Ile Val Arg Gly Thr Gln Leu Phe Glu Asp Asn Tyr 
            100                 105                 110         


Ala Leu Ala Val Leu Asp Asn Gly Asp Pro Leu Asn Asn Thr Thr Pro 
        115                 120                 125             


Val Thr Gly Ala Ser Pro Gly Gly Leu Arg Glu Leu Gln Leu Arg Ser 
    130                 135                 140                 


Leu Thr Glu Ile Leu Lys Gly Gly Val Leu Ile Gln Arg Asn Pro Gln 
145                 150                 155                 160 


Leu Cys Tyr Gln Asp Thr Ile Leu Trp Lys Asp Ile Phe His Lys Asn 
                165                 170                 175     


Asn Gln Leu Ala Leu Thr Leu Ile Asp Thr Asn Arg Ser Arg Ala Cys 
            180                 185                 190         


His Pro Cys Ser Pro Met Cys Lys Gly Ser Arg Cys Trp Gly Glu Ser 
        195                 200                 205             


Ser Glu Asp Cys Gln Ser Leu Thr Arg Thr Val Cys Ala Gly Gly Cys 
    210                 215                 220                 


Ala Arg Cys Lys Gly Pro Leu Pro Thr Asp Cys Cys His Glu Gln Cys 
225                 230                 235                 240 


Ala Ala Gly Cys Thr Gly Pro Lys His Ser Asp Cys Leu Ala Cys Leu 
                245                 250                 255     


His Phe Asn His Ser Gly Ile Cys Glu Leu His Cys Pro Ala Leu Val 
            260                 265                 270         


Gln Tyr Ile Lys Ala Asn Ser Lys Phe Ile Gly Ile Thr Glu Leu Arg 
        275                 280                 285             


Tyr Thr Phe Gly Ala Ser Cys Val Thr Ala Cys Pro Tyr Asn Tyr Leu 
    290                 295                 300                 


Ser Thr Asp Val Gly Ser Cys Thr Leu Val Cys Pro Leu His Asn Gln 
305                 310                 315                 320 


Glu Val Thr Ala Glu Asp Gly Thr Gln Arg Cys Glu Lys Cys Ser Lys 
                325                 330                 335     


Pro Cys Ala Arg Val Cys Tyr Gly Leu Gly Met Glu His Leu Arg Glu 
            340                 345                 350         


Val Arg Ala Val Thr Ser Ala Asn Ile Gln Glu Phe Ala Gly Cys Lys 
        355                 360                 365             


Lys Ile Phe Gly Ser Leu Ala Phe Leu Pro Glu Ser Phe Asp Gly Asp 
    370                 375                 380                 


Pro Ala Ser Asn Thr Ala Pro Leu Gln Pro Glu Gln Leu Gln Val Phe 
385                 390                 395                 400 


Glu Thr Leu Glu Glu Ile Thr Gly Tyr Leu Tyr Ile Ser Ala Trp Pro 
                405                 410                 415     


Asp Ser Leu Pro Asp Leu Ser Val Phe Gln Asn Leu Gln Val Ile Arg 
            420                 425                 430         


Gly Arg Ile Leu His Asn Gly Ala Tyr Ser Leu Thr Leu Gln Gly Leu 
        435                 440                 445             


Gly Ile Ser Trp Leu Gly Leu Arg Ser Leu Arg Glu Leu Gly Ser Gly 
    450                 455                 460                 


Leu Ala Leu Ile His His Asn Thr His Leu Cys Phe Val His Thr Val 
465                 470                 475                 480 


Pro Trp Asp Gln Leu Phe Arg Asn Pro His Gln Ala Leu Leu His Thr 
                485                 490                 495     


Ala Asn Arg Pro Glu Asp Glu Cys Val Gly Glu Gly Leu Ala Cys His 
            500                 505                 510         


Gln Leu Cys Ala Arg Gly His Cys Trp Gly Pro Gly Pro Thr Gln Cys 
        515                 520                 525             


Val Asn Cys Ser Gln Phe Leu Arg Gly Gln Glu Cys Val Glu Glu Cys 
    530                 535                 540                 


Arg Val Leu Gln Gly Leu Pro Arg Glu Tyr Val Asn Ala Arg His Cys 
545                 550                 555                 560 


Leu Pro Cys His Pro Glu Cys Gln Pro Gln Asn Gly Ser Val Thr Cys 
                565                 570                 575     


Phe Gly Pro Glu Ala Asp Gln Cys Val Ala Cys Ala His Tyr Lys Asp 
            580                 585                 590         


Pro Pro Phe Cys Val Ala Arg Cys Pro Ser Gly Val Lys Pro Asp Leu 
        595                 600                 605             


Ser Tyr Met Pro Ile Trp Lys Phe Pro Asp Glu Glu Gly Ala Cys Gln 
    610                 615                 620                 


Pro Cys Pro Ile Asn Cys Thr His Ser Cys Val Asp Leu Asp Asp Lys 
625                 630                 635                 640 


Gly Cys Pro Ala Glu Gln Arg Ala Ser Pro Leu Thr Ser Phe Asn Asn 
                645                 650                 655     


Phe Thr Val Ser Phe Trp Leu Arg Val Pro Lys Val Ser Ala Ser His 
            660                 665                 670         


Leu Glu Ile Val Ser Ala Val Val Gly Ile Leu 
        675                 680             


