                         SEQUENCE LISTING

<110>  Research Foundation of the City University of New York
 
<120>  TCR(alpha)-LCR-DERIVED GENE REGULATORY CASSETTES

<130>  RESE.17336-RO

<150>  61/990,807
<151>  2014-05-09

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  4120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Entirely synthetic DNA sequence formed by standard recombinant 
       DNA technology from four functional regions of TCR(alpha) LCR.


<220>
<221>  misc_feature
<222>  (803)..(803)
<223>  n is a, c, g, or t

<400>  1
gcggccgctc tagaactagt ggatcccccc tctgtgtctc tcaccagcag taagtttgcc       60

atatatgggg aacagccctg tcgtgttccc ctgaagctga ttaccaaatg acccgtgctc      120

cagaagccct cccataggac tgcacgctgc cataggctga ggaatcacgc ttctgttgcc      180

cgggtcacac tggatcaaat cagtctcggg tgtagctgac tacccctatc ctggatgatt      240

gaggcttgcc cattcagtgg gtagtcggcc attagtggta ctggccactg ttggagctgt      300

ggaaaaggag actcccaggc cttaccccca agcgacagaa gcaaagcttt gcattttaac      360

acggtgccag gtggtggtgt gcacccggaa gtctgagatg cccgcatttc cagctctggg      420

tgtatcctgc aactacccgg aagcttaaca gagcttgagc ctctccattc tttattagct      480

ctttgcttag ctagacatac tccagagtga gttaacaagc cttttaggaa tggttgggtt      540

caatataatg acaaaacctg gcaggggtcc tgtgggccaa ggcctagcac tgaggagaag      600

ttcactagga tggctgagcg atcagtgtgc tcgacactcg tgcagacatc ctgcagcttt      660

ccccggagaa gtggatgagg tttactttgg attcaataag cagatatttc agatatgaaa      720

agcaatcgga ctttaagagc aaaggaagac gtcacaacag tacgcgtgaa gatttacatt      780

tctcgcagtg gcttagagcc acntgggccg ggtactagtt tccaggcgca gatctgcttt      840

tgtctcgggg ttgtgatttt ccccttggac tagccttggc ggcaggcgta gcttgttgtg      900

agtttgtacc gtttacaaca gtacaaacta ctttcagggg tcttgagttc aaatttgaaa      960

atgcaccata ggcttgctgt agactttagt tagcttgctc tgtggctctc agttccatac     1020

gcacagagag gctaaccgtg ggttattcct caactaggga agggccttcc acaaggctcc     1080

ccgggcggag gggttggttt tgctgactca ggtactgtat gggctgaggg atggatgtcc     1140

tgctgtttcc tgcagcttcc agctcacacc tcctctgtgt gtgtgtgtgg gggggggggt     1200

tatttttatt tcctgtgttc aatctgtggg gctgcaagcc atttaccaca actactgtac     1260

attgttcctt gctcggcagt ggtgggaaac agccctttgc accctctgcc tacacacttc     1320

ctgtcgccac ggcatgcaaa tgttcctcct gcttcctctc ccggcagcct gcacctctgc     1380

tcttgatcac gtctgtgatc ttggcccaca aagcggtctt gttacattgt tggaacatac     1440

ttgggaagtg acagagattg ttcccttctt tgggaaagtc agcaagccag ccagcatcag     1500

ctagtttcca gcacctggaa gctactagct cagttgtagt tctggtgggt tgtggactgg     1560

aagtctaggc tagtgtttgg gtgcctcatg acttaagatt tatcttaggg ttttcattgc     1620

tgtgataaaa cgccatgggg tggggtgggg ggtggaagcc catgggagga aagggtttgt     1680

ttcagtttac agctgaaatc catcagtcgg ggaagtcggg gcaggaatgc aaggcaggag     1740

ctgatgctca aaccaggggt gaaagctgcc tgctgcctta gaataccttg ctcagcctgc     1800

tttcttatag ctgggccagc ttgctcaggg aggcaccacc tacatgggtt gggcttccct     1860

catcagttac aagaaaatgc ccccacaggc ttgccatcaa ctttgagggg actttttcaa     1920

ttcgataaaa aattaaatat tgccgggcgt ggtccaccct ttattcccag cactcgggag     1980

gaacagaggc aggcggattt ctgagttcga ggccagcctg gtctacagag tgagtttcag     2040

gcatccaggg ctacacagag aaaccctgcc tggaaaaaaa aaaaagaaag aaaaattatg     2100

tcatttccct ttcacctcct tctccctctc atctacttcc tcctccaata ccctcccaat     2160

actttctcaa attcatggcc tctggtttta attgtgatag gtgtgtgtat gtttatgcac     2220

acacacacac acacacacac acacacacac acacacactt atgatttcag agctgaccat     2280

ctggtgttgg atagctggtt gggggcttct ccctggggaa gaatgtttcc cactgtcagt     2340

gttgcttggc tgctgcgctc cttgtttagg gttggagaat cccccctcac atttctaaga     2400

ccaatctcag agcacacttc ctgtttctct ggttcttaca gtcgttgtgc ccccaacccc     2460

cttctaagat gttccctgcg cctcgggtgc aggagctggg ttgtcgatag atccgctgga     2520

gccgggcacc ccaggatcca cggtcctctg cctctgacag ggtgtggttt tctttctgta     2580

tgcgtgcgtg cgcgtttgtg agtgtgtcag aggacatctt ggcaggagtc ggttctttcc     2640

ttgtaccatg taggtactaa ggttcaagct tgggtcacca gggcgtcact aggtgccttg     2700

tatacagagt catcttgtct tctgattttg ccttatcact gaacttctga ccacggggct     2760

ggcaaggtgg ctcagagagt aaaaacccct tctgagcagc cccggtgatc tgagattgac     2820

cccccccccc agcttccacg cagaggtaga gaactgacta ttgaagttct ctgaccgtca     2880

caggcacgcg ccacaccatc cccgccatga gactgagcct acagtttatg catctattat     2940

gctatgtatt ccctcagcag gggagccctg tccacccatg ctccctggag tggacagggc     3000

tcgggtttgc cattctaatt cagtcctaaa gaggaaccaa gtaagtgatc aagcttatcg     3060

atctgggcct gcgggccata ttccatctcc tggcttccaa cagggtagtt tctttctggg     3120

tttctcttgt agctaggtct cctaaacctg ggtaggcagt gcccctgctg cctgaaggcc     3180

tgaccctaga ctctttgcag gcagagagga tcacacaagt ctggagtcca gtgactggca     3240

cagggcagga ccctgtgaaa cagaagccac ttgtcatttt attttccttt ggaaagagtt     3300

tttccgtagg atgcagggat tttctttatc ccgaataaac acgccgaggg agaaagcctt     3360

ttggtggagt actgctgccc ccgtgtggct accttaagac actacaacag agtgggactc     3420

ccaagacgaa agtttttcag gtttcaggtt ctgcgacttc ctaggaccct gagagacaga     3480

tgtccactag ggttatctct tgacacttaa atttcctcct ggagtggaaa tgagagcgaa     3540

tggggcttct tgtggaaggt atctgttggg gaacgagcct tagctgtgtg tgtaggcagc     3600

tgcctctttc acaacctgtc cagcacaagc tggactggac atagactttc tcccttgaac     3660

taagtatata gacttgtcta atttgagggc aatagaggtc aggggagggg gacttcttgg     3720

gagtggagcc gcagtgcctc cccagccggc tgctgtgaat gggtgttacc accaagacct     3780

gcaagcccca cccaatgtgc tctccgtggc cttcttttct gcacctgtgg ttgggctgcg     3840

gtgtaacggc ccagcctacc tcttctgggc acgtggcccg gagagatctt atctctaact     3900

ctgtattttt aactcctctt tccagaggat gtggcttctg cgggagagct tcaaaggggg     3960

acctgtttgc ccatgtcagc agccgtgacg tcatggaagt gggaggctgt tcagacccaa     4020

acacctggag ggaagtggga aactttttcc atttcctgtt ctacttctgg ctattgattc     4080

actatctgac cagcttaccc atttcagggt gcagctcgag                           4120


<210>  2
<211>  2483
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Entirely synthetic DNA sequence formed by standard recombinant 
       DNA technology from four functional regions of TCR(alpha) LCR.

<400>  2
gcggccgctc tagaactagt gtgcttttgt ctcggggttg tgattttccc cttggactag       60

ccttggcggc aggcgtagct tgttgtgagt ttgtaccgtt tacaacagta caaactactt      120

tcaggggtct tgagttcaaa tttgaaaatg caccataggc ttgctgtaga ctttagttag      180

cttgctctgt ggctctcagt tccatacgca cagagaggct aaccgtgggt tattcctcaa      240

ctagggaagg gccttccaca aggctccccg ggcggagggg ttggttttgc tgactcaggt      300

actgtatggg ctgagggatg gatgtcctgc tgtttcctgc agcttccagc tcacacctcc      360

tctgtgtgtg tgtgtggggg ggggggttat ttttatttcc tgtgttcaat ctgtggggct      420

gcaagccatt taccacaact actgtacatt gttccttgct cggcagtggt gggaaacagc      480

cctttgcacc ctctgcctac acacttcctg tcgccacggc atgcaaatgt tcctcctgct      540

tcctctcccg gcagcctgca cctctgctct ggatcccccg ggctgcagga attcgataaa      600

aaattaaata ttgccgggcg tggtccaccc tttattccca gcactcggga ggaacagagg      660

caggcggatt tctgagttcg aggccagcct ggtctacaga gtgagtttca ggcatccagg      720

gctacacaga gaaaccctgc ctggaaaaaa aaaaaagaaa gaaaaattat gtcatttccc      780

tttcacctcc ttctccctct catctacttc ctcctccaat accctcccaa tactttctca      840

aattcatggc ctctggtttt aattgtgata ggtgtgtgta tgtttatgca cacacacaca      900

cacacacaca cacacacaca cacacacact tatgatttca gagctgacca tctggtgttg      960

gatagctggt tgggggcttc tccctgggga agaatgtttc ccactgtcag tgttgcttgg     1020

ctgctgcgct ccttgtttag ggttggagaa tcccccctca catttctaag accaatctca     1080

gagcacactt cctgtttctc tggttcttac agtcgttgtg cccccaaccc ccttctaaga     1140

tgttccctgc gcctcgggtg caggagctgg gttgtcgata gatccgctgg agccgggcac     1200

cccaggatcc acggtcctct gcctctgaca gggtgtggtt ttctttctgt atgcgtgcgt     1260

gcgcgtttgt gagtgtgtca gaggacatct tggcaggagt cggttctttc cttgtaccat     1320

gtaggtacta aggttcaagc ttgggtcacc agggcgtcac taggtgcctt gtatacagag     1380

tcatcttgtc ttctgatttt gccttatcac tgaacttctg accacggggc tggcaaggtg     1440

gctcagagag taaaaacccc ttctgagcag ccccggtgat ctgagattga cccccccccc     1500

cagcttccac gcagaggtag agaactgact attgaagttc tctgaccgtc acaggcacgc     1560

gccacaccat ccccgccatg agactgagcc tacagtttat gcatctatta tgctatgtat     1620

tccctcagca ggggagccct gtccacccat gctccctgga gtggacaggg ctcgggtttg     1680

ccattctaat tcagtcctaa agaggaacca agtaagtgat caagcttatc gatactgctg     1740

cccccgtgtg gctaccttaa gacactacaa cagagtggga ctcccaagac gaaagttttt     1800

caggtttcag gttctgcgac ttcctaggac cctgagagac agatgtccac tagggttatc     1860

tcttgacact taaatttcct cctggagtgg aaatgagagc gaatggggct tcttgtggaa     1920

ggtatctgtt ggggaacgag ccttagctgt gtgtgtaggc agctgcctct ttcacaacct     1980

gtccagcaca agctggactg gacatagact ttctcccttg aactaagtat atagacttgt     2040

ctaatttgag ggcaatagag gtcaggggag ggggacttct tgggagtgga gccgcagtgc     2100

ctccccagcc ggctgctgtg aatgggtgtt accaccaaga cctgcaagcc ccacccaatg     2160

tgctctccgt ggccttcttt tctgcacctg tggttgggct gcggtgtaac ggcccagcct     2220

acctcttctg ggcacgtggc ccggagagat cttatctcta actctgtatt tttaactcct     2280

ctttccagag gatgtggctt ctgcgggaga gcttcaaagg gggacctgtt tgcccatgtc     2340

agcagccgtg acgtcatgga agtgggaggc tgttcagacc caaacacctg gagggaagtg     2400

ggaaactttt tccatttcct gttctacttc tggctattga ttcactatct gaccagctta     2460

cccatttcag ggtgcagctc gag                                             2483


<210>  3
<211>  1362
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Entirely synthetic DNA sequence formed by standard recombinant 
       DNA technology from four functional regions of TCR(alpha) LCR.

<400>  3
gcggccgctc tagaactagt gtgcttttgt ctcggggttg tgattttccc cttggactag       60

ccttggcggc aggcgtagct tgttgtgagt ttgtaccgtt tacaacagta caaactactt      120

tcaggggtct tgagttcaaa tttgaaaatg caccataggc ttgctgtaga ctttagttag      180

cttgctctgt ggctctcagt tccatacgca cagagaggct aaccgtgggt tattcctcaa      240

ctagggaagg gccttccaca aggctccccg ggcggagggg ttggttttgc tgactcaggt      300

actgtatggg ctgagggatg gatgtcctgc tgtttcctgc agcttccagc tcacacctcc      360

tctgtgtgtg tgtgtggggg ggggggttat ttttatttcc tgtgttcaat ctgtggggct      420

gcaagccatt taccacaact actgtacatt gttccttgct cggcagtggt gggaaacagc      480

cctttgcacc ctctgcctac acacttcctg tcgccacggc atgcaaatgt tcctcctgct      540

tcctctcccg gcagcctgca cctctgctct ggatcccccg ggctgcagga attcgatatc      600

aagcttatcg atactgctgc ccccgtgtgg ctaccttaag acactacaac agagtgggac      660

tcccaagacg aaagtttttc aggtttcagg ttctgcgact tcctaggacc ctgagagaca      720

gatgtccact agggttatct cttgacactt aaatttcctc ctggagtgga aatgagagcg      780

aatggggctt cttgtggaag gtatctgttg gggaacgagc cttagctgtg tgtgtaggca      840

gctgcctctt tcacaacctg tccagcacaa gctggactgg acatagactt tctcccttga      900

actaagtata tagacttgtc taatttgagg gcaatagagg tcaggggagg gggacttctt      960

gggagtggag ccgcagtgcc tccccagccg gctgctgtga atgggtgtta ccaccaagac     1020

ctgcaagccc cacccaatgt gctctccgtg gccttctttt ctgcacctgt ggttgggctg     1080

cggtgtaacg gcccagccta cctcttctgg gcacgtggcc cggagagatc ttatctctaa     1140

ctctgtattt ttaactcctc tttccagagg atgtggcttc tgcgggagag cttcaaaggg     1200

ggacctgttt gcccatgtca gcagccgtga cgtcatggaa gtgggaggct gttcagaccc     1260

aaacacctgg agggaagtgg gaaacttttt ccatttcctg ttctacttct ggctattgat     1320

tcactatctg accagcttac ccatttcagg gtgcagctcg ag                        1362


<210>  4
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synethic primer sequence

<400>  4
gacctgctcg agctgcaccc tgaaatggt                                         29


<210>  5
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer sequence

<400>  5
cgagtcatcg atactgctgc ccccgtgtg                                         29


