                         SEQUENCE LISTING

<110>  GlaxoSmithKline Biologicals SA
 
<120>  PURIFICATION OF SECRETED POLYSACCHARIDES FROM S. AGALACTIAE

<130>  VN56082

<160>  41    

<170>  PatentIn version 3.5

<210>  1
<211>  1455
<212>  DNA
<213>  Streptococcus agalactiae

<400>  1
atgtctaatc attcgcgccg tcaacaaaag aaacactcac atacacctct acgggtgatt       60

aatttatttc ttttggtgat ttttattttg ttaagtgtag tctcattatt tcttatgtat      120

cgtcaccatt ttttggcatt tagacacctg aacgtcattt atggagttgt aattgtttta      180

atcattttag caagtttatt tctttgtatt aagaataaag ctagaatttt tacaactata      240

attttagtat tagcttctat tttcgttgct actactttat atggatttaa gtcaaccatt      300

gatttgacaa ataatctaaa taaaactgct tcatactctg aaattgagat gagtgtaatt      360

gtaccaaaag attctaaaat aaccaatata gaagctgtca gcaaattagc cgcaccagtt      420

aaaaacgata cttcaaatat tactgatttg atagaacata taaaatcaga aaaaggaatc      480

tctattacac cacaaaaaac agattcttac caggatgcat acaatagaat taaaaatggt      540

gatagtcagg ctatggtttt aaataatgct tatgttagct taattgaact tagcacccct      600

gattttaaat cgcagataaa aacgatttat acttacaaaa ttaagaaaaa aattaatcgt      660

aaaaatacta atcataaaga aggggtattt aatatctata ttagcggtat tgatactttt      720

ggctctatat caacagtatc aagatctgat gtaaatatta ttatgacggt taataccaat      780

acccacaaag tattgttaac gacaacacca cgagatgcct atgtaaaaat tccagatggt      840

gggggcaatc aatatgataa attaacccat gcaggtttgt atggcgttga gacatcaatg      900

aaaacacttg aaaaccttta cgacatcaac cttgattatt atgctagaat taatttttca      960

tcatttttaa aattaataga cctcttggga ggagtgacag tttataacga tcaagctttt     1020

acaagtaaac atggtaattt tgacttccct gttggtcaag taacattgaa ttctgagcag     1080

gctttgggct ttgttagaga acgttattct ctacaaggag gcgataacga tagaggtaga     1140

aatcaagaaa aagtgattgc agctattata aataagttag cttctagtca gtcagtaaca     1200

aaattaaata gcattacctc acagctccaa acgtccgttc aaactaatat gactattgat     1260

aatattaatg atttgattaa caatcaattg tcaactggac aacgcttcac tgtcgagtca     1320

caagcattaa ctggtcatgg ttcaacgggt gaactccctt catatgcaat gccaggagct     1380

caactttata tgatgtcaat tgatcaatct agcttatcta atgcaaaatc aaaaattaag     1440

aacacaatgg aggaa                                                      1455


<210>  2
<211>  485
<212>  PRT
<213>  Streptococcus agalactiae

<400>  2

Met Ser Asn His Ser Arg Arg Gln Gln Lys Lys His Ser His Thr Pro 
1               5                   10                  15      


Leu Arg Val Ile Asn Leu Phe Leu Leu Val Ile Phe Ile Leu Leu Ser 
            20                  25                  30          


Val Val Ser Leu Phe Leu Met Tyr Arg His His Phe Leu Ala Phe Arg 
        35                  40                  45              


His Leu Asn Val Ile Tyr Gly Val Val Ile Val Leu Ile Ile Leu Ala 
    50                  55                  60                  


Ser Leu Phe Leu Cys Ile Lys Asn Lys Ala Arg Ile Phe Thr Thr Ile 
65                  70                  75                  80  


Ile Leu Val Leu Ala Ser Ile Phe Val Ala Thr Thr Leu Tyr Gly Phe 
                85                  90                  95      


Lys Ser Thr Ile Asp Leu Thr Asn Asn Leu Asn Lys Thr Ala Ser Tyr 
            100                 105                 110         


Ser Glu Ile Glu Met Ser Val Ile Val Pro Lys Asp Ser Lys Ile Thr 
        115                 120                 125             


Asn Ile Glu Ala Val Ser Lys Leu Ala Ala Pro Val Lys Asn Asp Thr 
    130                 135                 140                 


Ser Asn Ile Thr Asp Leu Ile Glu His Ile Lys Ser Glu Lys Gly Ile 
145                 150                 155                 160 


Ser Ile Thr Pro Gln Lys Thr Asp Ser Tyr Gln Asp Ala Tyr Asn Arg 
                165                 170                 175     


Ile Lys Asn Gly Asp Ser Gln Ala Met Val Leu Asn Asn Ala Tyr Val 
            180                 185                 190         


Ser Leu Ile Glu Leu Ser Thr Pro Asp Phe Lys Ser Gln Ile Lys Thr 
        195                 200                 205             


Ile Tyr Thr Tyr Lys Ile Lys Lys Lys Ile Asn Arg Lys Asn Thr Asn 
    210                 215                 220                 


His Lys Glu Gly Val Phe Asn Ile Tyr Ile Ser Gly Ile Asp Thr Phe 
225                 230                 235                 240 


Gly Ser Ile Ser Thr Val Ser Arg Ser Asp Val Asn Ile Ile Met Thr 
                245                 250                 255     


Val Asn Thr Asn Thr His Lys Val Leu Leu Thr Thr Thr Pro Arg Asp 
            260                 265                 270         


Ala Tyr Val Lys Ile Pro Asp Gly Gly Gly Asn Gln Tyr Asp Lys Leu 
        275                 280                 285             


Thr His Ala Gly Leu Tyr Gly Val Glu Thr Ser Met Lys Thr Leu Glu 
    290                 295                 300                 


Asn Leu Tyr Asp Ile Asn Leu Asp Tyr Tyr Ala Arg Ile Asn Phe Ser 
305                 310                 315                 320 


Ser Phe Leu Lys Leu Ile Asp Leu Leu Gly Gly Val Thr Val Tyr Asn 
                325                 330                 335     


Asp Gln Ala Phe Thr Ser Lys His Gly Asn Phe Asp Phe Pro Val Gly 
            340                 345                 350         


Gln Val Thr Leu Asn Ser Glu Gln Ala Leu Gly Phe Val Arg Glu Arg 
        355                 360                 365             


Tyr Ser Leu Gln Gly Gly Asp Asn Asp Arg Gly Arg Asn Gln Glu Lys 
    370                 375                 380                 


Val Ile Ala Ala Ile Ile Asn Lys Leu Ala Ser Ser Gln Ser Val Thr 
385                 390                 395                 400 


Lys Leu Asn Ser Ile Thr Ser Gln Leu Gln Thr Ser Val Gln Thr Asn 
                405                 410                 415     


Met Thr Ile Asp Asn Ile Asn Asp Leu Ile Asn Asn Gln Leu Ser Thr 
            420                 425                 430         


Gly Gln Arg Phe Thr Val Glu Ser Gln Ala Leu Thr Gly His Gly Ser 
        435                 440                 445             


Thr Gly Glu Leu Pro Ser Tyr Ala Met Pro Gly Ala Gln Leu Tyr Met 
    450                 455                 460                 


Met Ser Ile Asp Gln Ser Ser Leu Ser Asn Ala Lys Ser Lys Ile Lys 
465                 470                 475                 480 


Asn Thr Met Glu Glu 
                485 


<210>  3
<211>  222
<212>  PRT
<213>  Streptococcus agalactiae

<400>  3

Ile Asp Thr Phe Gly Ser Ile Ser Thr Val Ser Arg Ser Asp Val Asn 
1               5                   10                  15      


Ile Ile Met Thr Val Asn Thr Asn Thr His Lys Val Leu Leu Thr Thr 
            20                  25                  30          


Thr Pro Arg Asp Ala Tyr Val Lys Ile Pro Asp Gly Gly Gly Asn Gln 
        35                  40                  45              


Tyr Asp Lys Leu Thr His Ala Gly Leu Tyr Gly Val Glu Thr Ser Met 
    50                  55                  60                  


Lys Thr Leu Glu Asn Leu Tyr Asp Ile Asn Leu Asp Tyr Tyr Ala Arg 
65                  70                  75                  80  


Ile Asn Phe Ser Ser Phe Leu Lys Leu Ile Asp Leu Leu Gly Gly Val 
                85                  90                  95      


Thr Val Tyr Asn Asp Gln Ala Phe Thr Ser Lys His Gly Asn Phe Asp 
            100                 105                 110         


Phe Pro Val Gly Gln Val Thr Leu Asn Ser Glu Gln Ala Leu Gly Phe 
        115                 120                 125             


Val Arg Glu Arg Tyr Ser Leu Gln Gly Gly Asp Asn Asp Arg Gly Arg 
    130                 135                 140                 


Asn Gln Glu Lys Val Ile Ala Ala Ile Ile Asn Lys Leu Ala Ser Ser 
145                 150                 155                 160 


Gln Ser Val Thr Lys Leu Asn Ser Ile Thr Ser Gln Leu Gln Thr Ser 
                165                 170                 175     


Val Gln Thr Asn Met Thr Ile Asp Asn Ile Asn Asp Leu Ile Asn Asn 
            180                 185                 190         


Gln Leu Ser Thr Gly Gln Arg Phe Thr Val Glu Ser Gln Ala Leu Thr 
        195                 200                 205             


Gly His Gly Ser Thr Gly Glu Leu Pro Ser Tyr Ala Met Pro 
    210                 215                 220         


<210>  4
<211>  711
<212>  DNA
<213>  Streptococcus agalactiae

<400>  4
atgtctaatc attcgcgccg tcaacaaaag aaacactcac atacacctct acgggtgatt       60

aatttatttc ttttggtgat ttttattttg ttaagtgtag tctcattatt tcttatgtat      120

cgtcaccatt ttttggcatt tagacacctg aacgtcattt atggagttgt aattgtttta      180

atcattttag caagtttatt tctttgtatt aagaataaag ctagaatttt tacaactata      240

attttagtat tagcttctat tttcgttgct actactttat atggatttaa gtcaaccatt      300

gatttgacaa ataatctaaa taaaactgct tcatactctg aaattgagat gagtgtaatt      360

gtaccaaaag attctaaaat aaccaatata gaagctgtca gcaaattagc cgcaccagtt      420

aaaaacgata cttcaaatat tactgatttg atagaacata taaaatcaga aaaaggaatc      480

tctattacac cacaaaaaac agattcttac caggatgcat acaatagaat taaaaatggt      540

gatagtcagg ctatggtttt aaataatgct tatgttagct taattgaact tagcacccct      600

gattttaaat cgcagataaa aacgatttat acttacaaaa ttaagaaaaa aattaatcgt      660

aaaaatacta atcataaaga aggggtattt aatatctata ttagcggtta a               711


<210>  5
<211>  236
<212>  PRT
<213>  Streptococcus agalactiae

<400>  5

Met Ser Asn His Ser Arg Arg Gln Gln Lys Lys His Ser His Thr Pro 
1               5                   10                  15      


Leu Arg Val Ile Asn Leu Phe Leu Leu Val Ile Phe Ile Leu Leu Ser 
            20                  25                  30          


Val Val Ser Leu Phe Leu Met Tyr Arg His His Phe Leu Ala Phe Arg 
        35                  40                  45              


His Leu Asn Val Ile Tyr Gly Val Val Ile Val Leu Ile Ile Leu Ala 
    50                  55                  60                  


Ser Leu Phe Leu Cys Ile Lys Asn Lys Ala Arg Ile Phe Thr Thr Ile 
65                  70                  75                  80  


Ile Leu Val Leu Ala Ser Ile Phe Val Ala Thr Thr Leu Tyr Gly Phe 
                85                  90                  95      


Lys Ser Thr Ile Asp Leu Thr Asn Asn Leu Asn Lys Thr Ala Ser Tyr 
            100                 105                 110         


Ser Glu Ile Glu Met Ser Val Ile Val Pro Lys Asp Ser Lys Ile Thr 
        115                 120                 125             


Asn Ile Glu Ala Val Ser Lys Leu Ala Ala Pro Val Lys Asn Asp Thr 
    130                 135                 140                 


Ser Asn Ile Thr Asp Leu Ile Glu His Ile Lys Ser Glu Lys Gly Ile 
145                 150                 155                 160 


Ser Ile Thr Pro Gln Lys Thr Asp Ser Tyr Gln Asp Ala Tyr Asn Arg 
                165                 170                 175     


Ile Lys Asn Gly Asp Ser Gln Ala Met Val Leu Asn Asn Ala Tyr Val 
            180                 185                 190         


Ser Leu Ile Glu Leu Ser Thr Pro Asp Phe Lys Ser Gln Ile Lys Thr 
        195                 200                 205             


Ile Tyr Thr Tyr Lys Ile Lys Lys Lys Ile Asn Arg Lys Asn Thr Asn 
    210                 215                 220                 


His Lys Glu Gly Val Phe Asn Ile Tyr Ile Ser Gly 
225                 230                 235     


<210>  6
<211>  48
<212>  DNA
<213>  Streptococcus agalactiae

<400>  6
atgtctaatc attcgcgccg tcaacaaaag aacacaatgg aggaataa                    48


<210>  7
<211>  15
<212>  PRT
<213>  Streptococcus agalactiae

<400>  7

Met Ser Asn His Ser Arg Arg Gln Gln Lys Asn Thr Met Glu Glu 
1               5                   10                  15  


<210>  8
<211>  288
<212>  DNA
<213>  Streptococcus agalactiae

<400>  8
atgtctaatc attcgcgccg tcaacaaaag aaacactcac atacacctct acgggtgatt       60

aatttatttc ttttggtgat ttttattttg ttaagtgtag tctcattatt tcttatgtat      120

cgtcaccatt ttttggcatt tagacacctg aacgtcattt atggagttgt aattgtttta      180

atcattttag caagtttatt tctttgtatt aagaataaag ctagaatttt tacaactata      240

attttagtat tagcttctat tttcgttgct actactttat atggataa                   288


<210>  9
<211>  95
<212>  PRT
<213>  Streptococcus agalactiae

<400>  9

Met Ser Asn His Ser Arg Arg Gln Gln Lys Lys His Ser His Thr Pro 
1               5                   10                  15      


Leu Arg Val Ile Asn Leu Phe Leu Leu Val Ile Phe Ile Leu Leu Ser 
            20                  25                  30          


Val Val Ser Leu Phe Leu Met Tyr Arg His His Phe Leu Ala Phe Arg 
        35                  40                  45              


His Leu Asn Val Ile Tyr Gly Val Val Ile Val Leu Ile Ile Leu Ala 
    50                  55                  60                  


Ser Leu Phe Leu Cys Ile Lys Asn Lys Ala Arg Ile Phe Thr Thr Ile 
65                  70                  75                  80  


Ile Leu Val Leu Ala Ser Ile Phe Val Ala Thr Thr Leu Tyr Gly 
                85                  90                  95  


<210>  10
<211>  699
<212>  DNA
<213>  Streptococcus agalactiae

<400>  10
atgactcgtt tagaaatagt tgatagcaag ttaagacaag caaaaaaaac agaagaatac       60

ttcaatgcga tccgtacaaa catacagttt agtggaaagg aaaataaaat tcttgcaatt      120

acctctgtta gggaagggga aggaaaatcc actacttcaa caagtttagc tttatcttta      180

gctcaagcag gatttaaaac attattaatt gatgcggata ctaggaactc tgttatgtct      240

ggaaccttta aagcaactgg aactattaaa ggcttgacga attatttatc aggtaatgca      300

gatcttggag atattatctg tgaaaccaat gttcctagac tgatggtcgt tccttcaggg      360

aaagtaccac caaatccaac agcattactt cagaacgctt attttaataa gatgattgaa      420

gctattaaaa atatatttga ttatattatc atcgatactc cacctattgg tttagttgtt      480

gatgccgcaa taatctctaa tgcttgcgat ggttttattt tagtaaccca agcaggtaga      540

ataaaacgta attatgttga aaaagcaaaa gaacagatgg aacaaagtgg ttcaaagttc      600

ttaggtatta ttcttaataa agttagtgaa tctgttgcta cttacggcga ttacggcgat      660

tatggaaatt acggaaaaag ggatagaaaa aggaagtaa                             699


<210>  11
<211>  232
<212>  PRT
<213>  Streptococcus agalactiae

<400>  11

Met Thr Arg Leu Glu Ile Val Asp Ser Lys Leu Arg Gln Ala Lys Lys 
1               5                   10                  15      


Thr Glu Glu Tyr Phe Asn Ala Ile Arg Thr Asn Ile Gln Phe Ser Gly 
            20                  25                  30          


Lys Glu Asn Lys Ile Leu Ala Ile Thr Ser Val Arg Glu Gly Glu Gly 
        35                  40                  45              


Lys Ser Thr Thr Ser Thr Ser Leu Ala Leu Ser Leu Ala Gln Ala Gly 
    50                  55                  60                  


Phe Lys Thr Leu Leu Ile Asp Ala Asp Thr Arg Asn Ser Val Met Ser 
65                  70                  75                  80  


Gly Thr Phe Lys Ala Thr Gly Thr Ile Lys Gly Leu Thr Asn Tyr Leu 
                85                  90                  95      


Ser Gly Asn Ala Asp Leu Gly Asp Ile Ile Cys Glu Thr Asn Val Pro 
            100                 105                 110         


Arg Leu Met Val Val Pro Ser Gly Lys Val Pro Pro Asn Pro Thr Ala 
        115                 120                 125             


Leu Leu Gln Asn Ala Tyr Phe Asn Lys Met Ile Glu Ala Ile Lys Asn 
    130                 135                 140                 


Ile Phe Asp Tyr Ile Ile Ile Asp Thr Pro Pro Ile Gly Leu Val Val 
145                 150                 155                 160 


Asp Ala Ala Ile Ile Ser Asn Ala Cys Asp Gly Phe Ile Leu Val Thr 
                165                 170                 175     


Gln Ala Gly Arg Ile Lys Arg Asn Tyr Val Glu Lys Ala Lys Glu Gln 
            180                 185                 190         


Met Glu Gln Ser Gly Ser Lys Phe Leu Gly Ile Ile Leu Asn Lys Val 
        195                 200                 205             


Ser Glu Ser Val Ala Thr Tyr Gly Asp Tyr Gly Asp Tyr Gly Asn Tyr 
    210                 215                 220                 


Gly Lys Arg Asp Arg Lys Arg Lys 
225                 230         


<210>  12
<211>  51
<212>  DNA
<213>  Streptococcus agalactiae

<400>  12
atgactcgtt tagaaatagt tgatagcaaa aaggatagaa aaaggaagta a                51


<210>  13
<211>  16
<212>  PRT
<213>  Streptococcus agalactiae

<400>  13

Met Thr Arg Leu Glu Ile Val Asp Ser Lys Lys Asp Arg Lys Arg Lys 
1               5                   10                  15      


<210>  14
<211>  699
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CpsD sequence comprising K49A mutation

<400>  14
atgactcgtt tagaaatagt tgatagcaag ttaagacaag caaaaaaaac agaagaatac       60

ttcaatgcga tccgtacaaa catacagttt agtggaaagg aaaataaaat tcttgcaatt      120

acctctgtta gggaagggga aggagcatcc actacttcaa caagtttagc tttatcttta      180

gctcaagcag gatttaaaac attattaatt gatgcggata ctaggaactc tgttatgtct      240

ggaaccttta aagcaactgg aactattaaa ggcttgacga attatttatc aggtaatgca      300

gatcttggag atattatctg tgaaaccaat gttcctagac tgatggtcgt tccttcaggg      360

aaagtaccac caaatccaac agcattactt cagaacgctt attttaataa gatgattgaa      420

gctattaaaa atatatttga ttatattatc atcgatactc cacctattgg tttagttgtt      480

gatgccgcaa taatctctaa tgcttgcgat ggttttattt tagtaaccca agcaggtaga      540

ataaaacgta attatgttga aaaagcaaaa gaacagatgg aacaaagtgg ttcaaagttc      600

ttaggtatta ttcttaataa agttagtgaa tctgttgcta cttacggcga ttacggcgat      660

tatggaaatt acggaaaaag ggatagaaaa aggaagtaa                             699


<210>  15
<211>  232
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CpsD amino acid sequence comprising K49A mutation

<400>  15

Met Thr Arg Leu Glu Ile Val Asp Ser Lys Leu Arg Gln Ala Lys Lys 
1               5                   10                  15      


Thr Glu Glu Tyr Phe Asn Ala Ile Arg Thr Asn Ile Gln Phe Ser Gly 
            20                  25                  30          


Lys Glu Asn Lys Ile Leu Ala Ile Thr Ser Val Arg Glu Gly Glu Gly 
        35                  40                  45              


Ala Ser Thr Thr Ser Thr Ser Leu Ala Leu Ser Leu Ala Gln Ala Gly 
    50                  55                  60                  


Phe Lys Thr Leu Leu Ile Asp Ala Asp Thr Arg Asn Ser Val Met Ser 
65                  70                  75                  80  


Gly Thr Phe Lys Ala Thr Gly Thr Ile Lys Gly Leu Thr Asn Tyr Leu 
                85                  90                  95      


Ser Gly Asn Ala Asp Leu Gly Asp Ile Ile Cys Glu Thr Asn Val Pro 
            100                 105                 110         


Arg Leu Met Val Val Pro Ser Gly Lys Val Pro Pro Asn Pro Thr Ala 
        115                 120                 125             


Leu Leu Gln Asn Ala Tyr Phe Asn Lys Met Ile Glu Ala Ile Lys Asn 
    130                 135                 140                 


Ile Phe Asp Tyr Ile Ile Ile Asp Thr Pro Pro Ile Gly Leu Val Val 
145                 150                 155                 160 


Asp Ala Ala Ile Ile Ser Asn Ala Cys Asp Gly Phe Ile Leu Val Thr 
                165                 170                 175     


Gln Ala Gly Arg Ile Lys Arg Asn Tyr Val Glu Lys Ala Lys Glu Gln 
            180                 185                 190         


Met Glu Gln Ser Gly Ser Lys Phe Leu Gly Ile Ile Leu Asn Lys Val 
        195                 200                 205             


Ser Glu Ser Val Ala Thr Tyr Gly Asp Tyr Gly Asp Tyr Gly Asn Tyr 
    210                 215                 220                 


Gly Lys Arg Asp Arg Lys Arg Lys 
225                 230         


<210>  16
<211>  663
<212>  DNA
<213>  Streptococcus agalactiae

<400>  16
atgactcgtt tagaaatagt tgatagcaag ttaagacaag caaaaaaaac agaagaatac       60

ttcaatgcga tccgtacaaa catacagttt agtggaaagg aaaataaaat tcttgcaatt      120

acctctgtta gggaagggga aggaaaatcc actacttcaa caagtttagc tttatcttta      180

gctcaagcag gatttaaaac attattaatt gatgcggata ctaggaactc tgttatgtct      240

ggaaccttta aagcaactgg aactattaaa ggcttgacga attatttatc aggtaatgca      300

gatcttggag atattatctg tgaaaccaat gttcctagac tgatggtcgt tccttcaggg      360

aaagtaccac caaatccaac agcattactt cagaacgctt attttaataa gatgattgaa      420

gctattaaaa atatatttga ttatattatc atcgatactc cacctattgg tttagttgtt      480

gatgccgcaa taatctctaa tgcttgcgat ggttttattt tagtaaccca agcaggtaga      540

ataaaacgta attatgttga aaaagcaaaa gaacagatgg aacaaagtgg ttcaaagttc      600

ttaggtatta ttcttaataa agttagtgaa tctgttggaa aaagggatag aaaaaggaag      660

taa                                                                    663


<210>  17
<211>  220
<212>  PRT
<213>  Streptococcus agalactiae

<400>  17

Met Thr Arg Leu Glu Ile Val Asp Ser Lys Leu Arg Gln Ala Lys Lys 
1               5                   10                  15      


Thr Glu Glu Tyr Phe Asn Ala Ile Arg Thr Asn Ile Gln Phe Ser Gly 
            20                  25                  30          


Lys Glu Asn Lys Ile Leu Ala Ile Thr Ser Val Arg Glu Gly Glu Gly 
        35                  40                  45              


Lys Ser Thr Thr Ser Thr Ser Leu Ala Leu Ser Leu Ala Gln Ala Gly 
    50                  55                  60                  


Phe Lys Thr Leu Leu Ile Asp Ala Asp Thr Arg Asn Ser Val Met Ser 
65                  70                  75                  80  


Gly Thr Phe Lys Ala Thr Gly Thr Ile Lys Gly Leu Thr Asn Tyr Leu 
                85                  90                  95      


Ser Gly Asn Ala Asp Leu Gly Asp Ile Ile Cys Glu Thr Asn Val Pro 
            100                 105                 110         


Arg Leu Met Val Val Pro Ser Gly Lys Val Pro Pro Asn Pro Thr Ala 
        115                 120                 125             


Leu Leu Gln Asn Ala Tyr Phe Asn Lys Met Ile Glu Ala Ile Lys Asn 
    130                 135                 140                 


Ile Phe Asp Tyr Ile Ile Ile Asp Thr Pro Pro Ile Gly Leu Val Val 
145                 150                 155                 160 


Asp Ala Ala Ile Ile Ser Asn Ala Cys Asp Gly Phe Ile Leu Val Thr 
                165                 170                 175     


Gln Ala Gly Arg Ile Lys Arg Asn Tyr Val Glu Lys Ala Lys Glu Gln 
            180                 185                 190         


Met Glu Gln Ser Gly Ser Lys Phe Leu Gly Ile Ile Leu Asn Lys Val 
        195                 200                 205             


Ser Glu Ser Val Gly Lys Arg Asp Arg Lys Arg Lys 
    210                 215                 220 


<210>  18
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  18
taaagcggcc gcctctatca ctgacaacaa tgg                                    33


<210>  19
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  19
tatcctcgag gaagaagtat attgtggcgt a                                      31


<210>  20
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  20
ttgttgacgg cgcgaatgat tagacattgt aa                                     32


<210>  21
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  21
tcgcgccgtc aacaaaagaa cacaatggag gaataac                                37


<210>  22
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  22
tccatataaa gtagtagcaa cgaaaataga agc                                    33


<210>  23
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  23
actactttat atggataaca agaatgattg atattcattc                             40


<210>  24
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  24
accgctaata tagatattaa ataccccttc tttatg                                 36


<210>  25
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  25
tctatattag cggttaacaa gaatgattga tattcattct c                           41


<210>  26
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  26
tttaggatcc caaaaagaac gggtgaagga a                                      31


<210>  27
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  27
tctactcgag ctaccattac gacctactct a                                      31


<210>  28
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  28
gctatcaact atttctaaac gagtcattat attctc                                 36


<210>  29
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  29
gaaatagttg atagcaaaag ggatagaaaa aggaagtaa                              39


<210>  30
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  30
ggaaggggaa ggagcatcca ctacttca                                          28


<210>  31
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  31
tgaagtagtg gatgctcctt ccccttcc                                          28


<210>  32
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  32
aacagattca ctaactttat taagaataat acctaagaac                             40


<210>  33
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  33
gttagtgaat ctgttggaaa aagggataga aaaagg                                 36


<210>  34
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  34
aggtttactt gtggcgcttg                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  35
tctgcttgag caatggtgtc                                                   20


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  36
tcaactggac aacgcttcac                                                   20


<210>  37
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  37
aagttgagct cctggcattg                                                   20


<210>  38
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  38
tgctcatatg tggcattgtg                                                   20


<210>  39
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR Primer

<400>  39
agaaaagata gccggtccac                                                   20


<210>  40
<211>  148
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CpsD LytR domain aa248-395

<400>  40

Arg Ser Asp Val Asn Ile Ile Met Thr Val Asn Thr Asn Thr His Lys 
1               5                   10                  15      


Val Leu Leu Thr Thr Thr Pro Arg Asp Ala Tyr Val Lys Ile Pro Asp 
            20                  25                  30          


Gly Gly Gly Asn Gln Tyr Asp Lys Leu Thr His Ala Gly Leu Tyr Gly 
        35                  40                  45              


Val Glu Thr Ser Met Lys Thr Leu Glu Asn Leu Tyr Asp Ile Asn Leu 
    50                  55                  60                  


Asp Tyr Tyr Ala Arg Ile Asn Phe Ser Ser Phe Leu Lys Leu Ile Asp 
65                  70                  75                  80  


Leu Leu Gly Gly Val Thr Val Tyr Asn Asp Gln Ala Phe Thr Ser Lys 
                85                  90                  95      


His Gly Asn Phe Asp Phe Pro Val Gly Gln Val Thr Leu Asn Ser Glu 
            100                 105                 110         


Gln Ala Leu Gly Phe Val Arg Glu Arg Tyr Ser Leu Gln Gly Gly Asp 
        115                 120                 125             


Asn Asp Arg Gly Arg Asn Gln Glu Lys Val Ile Ala Ala Ile Ile Asn 
    130                 135                 140                 


Lys Leu Ala Ser 
145             


<210>  41
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CpsD PFAM/PPF Domain aa72-187

<400>  41

Asn Lys Ala Arg Ile Phe Thr Thr Ile Ile Leu Val Leu Ala Ser Ile 
1               5                   10                  15      


Phe Val Ala Thr Thr Leu Tyr Gly Phe Lys Ser Thr Ile Asp Leu Thr 
            20                  25                  30          


Asn Asn Leu Asn Lys Thr Ala Ser Tyr Ser Glu Ile Glu Met Ser Val 
        35                  40                  45              


Ile Val Pro Lys Asp Ser Lys Ile Thr Asn Ile Glu Ala Val Ser Lys 
    50                  55                  60                  


Leu Ala Ala Pro Val Lys Asn Asp Thr Ser Asn Ile Thr Asp Leu Ile 
65                  70                  75                  80  


Glu His Ile Lys Ser Glu Lys Gly Ile Ser Ile Thr Pro Gln Lys Thr 
                85                  90                  95      


Asp Ser Tyr Gln Asp Ala Tyr Asn Arg Ile Lys Asn Gly Asp Ser Gln 
            100                 105                 110         


Ala Met Val Leu 
        115     


