                         SEQUENCE LISTING

<110>  National Health Research Institutes
 
<120>  ADENOVIRAL VECTOR-BASED VACCINE AGAINST ENTEROVIRUS INFECTION

<130>  NHR0008WO

<150>  61/985,803
<151>  2014-04-29

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  862
<212>  PRT
<213>  Enterovirus 71

<400>  1

Met Gly Ser Gln Val Ser Thr Gln Arg Ser Gly Ser His Glu Asn Ser 
1               5                   10                  15      


Asn Ser Ala Thr Glu Gly Ser Thr Ile Asn Tyr Thr Thr Ile Asn Tyr 
            20                  25                  30          


Tyr Lys Asp Ser Tyr Ala Ala Thr Ala Gly Lys Gln Ser Leu Lys Gln 
        35                  40                  45              


Asp Pro Asp Lys Phe Ala Asn Pro Val Lys Asp Ile Phe Thr Glu Met 
    50                  55                  60                  


Ala Ala Pro Leu Lys Ser Pro Ser Ala Glu Ala Cys Gly Tyr Ser Asp 
65                  70                  75                  80  


Arg Val Ala Gln Leu Thr Ile Gly Asn Ser Thr Ile Thr Thr Gln Glu 
                85                  90                  95      


Ala Ala Asn Ile Ile Val Gly Tyr Gly Glu Trp Pro Ser Tyr Cys Ser 
            100                 105                 110         


Asp Ser Asp Ala Thr Ala Val Asp Lys Pro Thr Arg Pro Asp Val Ser 
        115                 120                 125             


Val Asn Arg Phe Tyr Thr Leu Asp Thr Lys Leu Trp Glu Lys Ser Ser 
    130                 135                 140                 


Lys Gly Trp Tyr Trp Lys Phe Pro Asp Val Leu Thr Glu Thr Gly Val 
145                 150                 155                 160 


Phe Gly Gln Asn Ala Gln Phe His Tyr Leu Tyr Arg Ser Gly Phe Cys 
                165                 170                 175     


Ile His Val Gln Cys Asn Ala Ser Lys Phe His Gln Gly Ala Leu Leu 
            180                 185                 190         


Val Ala Val Leu Pro Glu Tyr Val Ile Gly Thr Val Ala Gly Gly Thr 
        195                 200                 205             


Gly Thr Glu Asp Ser His Pro Pro Tyr Lys Gln Thr Gln Pro Gly Ala 
    210                 215                 220                 


Asp Gly Phe Glu Leu Gln His Pro Tyr Val Leu Asp Ala Gly Ile Pro 
225                 230                 235                 240 


Ile Ser Gln Leu Thr Val Cys Pro His Gln Trp Ile Asn Leu Arg Thr 
                245                 250                 255     


Asn Asn Cys Ala Thr Ile Ile Val Pro Tyr Ile Asn Ala Leu Pro Phe 
            260                 265                 270         


Asp Ser Ala Leu Asn His Cys Asn Phe Gly Leu Leu Val Val Pro Ile 
        275                 280                 285             


Ser Pro Leu Asp Tyr Asp Gln Gly Ala Thr Pro Val Ile Pro Ile Thr 
    290                 295                 300                 


Ile Thr Leu Ala Pro Met Cys Phe Glu Phe Ala Gly Phe Arg Gln Ala 
305                 310                 315                 320 


Val Thr Gln Gly Phe Pro Thr Glu Leu Lys Pro Gly Thr Asn Gln Phe 
                325                 330                 335     


Leu Thr Thr Asp Asp Gly Val Ser Ala Pro Ile Leu Pro Asn Phe His 
            340                 345                 350         


Pro Thr Pro Cys Ile His Ile Pro Gly Glu Val Arg Asn Leu Leu Glu 
        355                 360                 365             


Leu Cys Gln Val Glu Thr Ile Leu Glu Val Asn Asn Val Pro Thr Asn 
    370                 375                 380                 


Ala Thr Ser Leu Met Glu Arg Leu Arg Phe Pro Val Ser Ala Gln Ala 
385                 390                 395                 400 


Gly Lys Gly Glu Leu Cys Ala Val Phe Arg Ala Asp Pro Gly Arg Ser 
                405                 410                 415     


Gly Pro Trp Gln Ser Thr Leu Leu Gly Gln Leu Cys Gly Tyr Tyr Thr 
            420                 425                 430         


Gln Trp Ser Gly Ser Leu Glu Val Thr Phe Met Phe Thr Gly Ser Phe 
        435                 440                 445             


Met Ala Thr Gly Lys Met Leu Ile Ala Tyr Thr Pro Pro Gly Gly Pro 
    450                 455                 460                 


Leu Pro Lys Asp Arg Ala Thr Ala Met Leu Gly Thr Asp Val Ile Trp 
465                 470                 475                 480 


Asp Phe Gly Leu Gln Ser Ser Val Thr Leu Val Ile Pro Trp Ile Ser 
                485                 490                 495     


Asn Thr His Tyr Arg Ala His Ala Arg Asp Gly Val Phe Asp Tyr Tyr 
            500                 505                 510         


Thr Thr Gly Leu Val Ser Ile Trp Tyr Gln Thr Asn Tyr Val Val Pro 
        515                 520                 525             


Ile Gly Ala Pro Asn Thr Ala Tyr Ile Ile Ala Leu Ala Ala Ala Gln 
    530                 535                 540                 


Lys Asn Phe Thr Met Lys Leu Cys Lys Asp Ala Ser Asp Ile Leu Gln 
545                 550                 555                 560 


Thr Gly Thr Ile Gln Gly Asp Arg Val Ala Asp Val Ile Glu Ser Ser 
                565                 570                 575     


Ile Gly Asp Ser Val Ser Arg Ala Leu Thr Arg Ala Leu Pro Ala Pro 
            580                 585                 590         


Thr Gly Gln Asp Thr Gln Val Ser Ser His Arg Leu Asp Thr Gly Lys 
        595                 600                 605             


Gly Pro Ala Leu Gln Ala Ala Glu Ile Gly Ala Ser Ser Asn Ala Ser 
    610                 615                 620                 


Asp Glu Ser Met Ile Glu Thr Arg Cys Val Leu Asn Ser His Ser Thr 
625                 630                 635                 640 


Ala Glu Thr Thr Leu Asp Ser Phe Phe Ser Arg Ala Gly Leu Val Gly 
                645                 650                 655     


Glu Ile Asp Leu Pro Leu Glu Gly Thr Thr Asn Pro Asn Gly Tyr Ala 
            660                 665                 670         


Asn Trp Asp Ile Asp Ile Thr Gly Tyr Ala Gln Met Arg Arg Lys Val 
        675                 680                 685             


Glu Leu Phe Thr Tyr Met Arg Phe Asn Ala Glu Ile Thr Phe Val Ala 
    690                 695                 700                 


Cys Thr Pro Thr Gly Glu Val Val Pro Gln Leu Leu Gln Tyr Met Phe 
705                 710                 715                 720 


Val Pro Pro Gly Ala Pro Lys Pro Asp Ser Arg Glu Ser Leu Ala Trp 
                725                 730                 735     


Gln Thr Ala Thr Asn Pro Ser Val Phe Val Lys Leu Ser Asp Pro Pro 
            740                 745                 750         


Ala Gln Val Ser Val Pro Phe Met Ser Pro Ala Ser Ala Tyr Gln Trp 
        755                 760                 765             


Phe Tyr Asp Gly Tyr Pro Thr Phe Gly Glu His Lys Gln Glu Lys Asp 
    770                 775                 780                 


Leu Glu Tyr Gly Ala Cys Pro Asn Asn Met Met Gly Thr Phe Ser Val 
785                 790                 795                 800 


Arg Thr Val Gly Thr Ser Lys Ser Lys Cys Pro Leu Val Ile Arg Ile 
                805                 810                 815     


Tyr Met Arg Met Lys His Val Arg Ala Trp Ile Pro Arg Pro Met Arg 
            820                 825                 830         


Asn Gln Asn Tyr Leu Phe Lys Ala Asn Pro Asn Tyr Ala Gly Asn Ser 
        835                 840                 845             


Ile Lys Pro Thr Gly Ala Ser Arg Thr Ala Ile Thr Thr Leu 
    850                 855                 860         


<210>  2
<211>  645
<212>  PRT
<213>  Enterovirus 71

<400>  2

Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 
1               5                   10                  15      


Gln Val Gln Thr Asp Gln Gly His Phe Thr Met Leu Gly Val Arg Asp 
            20                  25                  30          


His Leu Ala Val Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 
        35                  40                  45              


Val Glu His Lys Leu Val Lys Ile Val Asp Ala Val Glu Leu Val Asp 
    50                  55                  60                  


Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Ile Thr Leu Asp Thr Asn 
65                  70                  75                  80  


Glu Lys Phe Arg Asp Ile Thr Arg Phe Ile Pro Glu Thr Ile Asn Pro 
                85                  90                  95      


Ala Ser Asp Ala Thr Leu Val Ile Asn Thr Glu His Met Pro Ser Met 
            100                 105                 110         


Phe Val Pro Val Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 
        115                 120                 125             


Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 
    130                 135                 140                 


Gly Gln Cys Gly Gly Val Val Thr Ala Val Gly Lys Val Ile Gly Ile 
145                 150                 155                 160 


His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Ala Leu Lys Arg 
                165                 170                 175     


Gly Tyr Phe Cys Ser Glu Gln Gly Glu Ile Gln Trp Met Lys Ser Asn 
            180                 185                 190         


Lys Glu Thr Gly Arg Leu Asn Ile Asn Gly Pro Thr Arg Thr Lys Leu 
        195                 200                 205             


Glu Pro Ser Val Phe His Asp Val Phe Glu Gly Thr Lys Glu Pro Ala 
    210                 215                 220                 


Val Leu Thr Ser Lys Asp Pro Arg Leu Glu Val Asp Phe Glu Gln Ala 
225                 230                 235                 240 


Leu Phe Ser Lys Tyr Val Gly Asn Thr Leu His Glu Pro Asp Glu Phe 
                245                 250                 255     


Val Lys Glu Ala Ala Leu His Tyr Ala Asn Gln Leu Lys Gln Leu Asp 
            260                 265                 270         


Ile Lys Thr Thr Lys Met Ser Met Glu Asp Ala Cys Tyr Gly Thr Glu 
        275                 280                 285             


Asn Leu Glu Ala Ile Asp Leu His Thr Ser Ala Gly Tyr Pro Tyr Ser 
    290                 295                 300                 


Ala Leu Gly Ile Lys Lys Lys Asp Ile Leu Asp Pro Thr Thr Arg Asp 
305                 310                 315                 320 


Val Ser Arg Met Lys Phe Tyr Met Asp Lys Tyr Gly Leu Asp Leu Pro 
                325                 330                 335     


Tyr Ser Thr Tyr Val Lys Asp Glu Leu Arg Ala Ile Asp Lys Ile Lys 
            340                 345                 350         


Lys Gly Lys Ser Arg Leu Ile Glu Ala Ser Ser Leu Asn Asp Ser Val 
        355                 360                 365             


Tyr Leu Arg Met Thr Phe Gly His Leu Tyr Glu Ala Phe His Ala Asn 
    370                 375                 380                 


Pro Gly Thr Val Thr Gly Ser Ala Val Gly Cys Asn Pro Asp Val Phe 
385                 390                 395                 400 


Trp Ser Lys Leu Pro Ile Leu Leu Pro Gly Ser Leu Phe Ala Phe Asp 
                405                 410                 415     


Tyr Ser Gly Tyr Asp Ala Ser Leu Ser Pro Val Trp Phe Arg Ala Leu 
            420                 425                 430         


Glu Ile Val Leu Arg Glu Ile Gly Tyr Ser Glu Asp Ala Val Ser Leu 
        435                 440                 445             


Ile Glu Gly Ile Asn His Thr His His Val Tyr Arg Asn Lys Thr Tyr 
    450                 455                 460                 


Cys Val Leu Gly Gly Met Pro Ser Gly Cys Ser Gly Thr Ser Ile Phe 
465                 470                 475                 480 


Asn Ser Met Ile Asn Asn Ile Ile Ile Arg Thr Leu Leu Ile Lys Thr 
                485                 490                 495     


Phe Lys Gly Ile Asp Leu Asp Glu Leu Asn Met Val Ala Tyr Gly Asp 
            500                 505                 510         


Asp Val Leu Ala Ser Tyr Pro Phe Pro Ile Asp Cys Leu Glu Leu Ala 
        515                 520                 525             


Arg Thr Gly Lys Glu Tyr Gly Leu Thr Met Thr Pro Ala Asp Lys Ser 
    530                 535                 540                 


Pro Cys Phe Asn Glu Val Thr Trp Glu Asn Ala Thr Phe Leu Lys Arg 
545                 550                 555                 560 


Gly Phe Leu Pro Asp His Gln Phe Pro Phe Leu Ile His Pro Thr Met 
                565                 570                 575     


Pro Met Arg Glu Ile His Glu Ser Ile Arg Trp Thr Lys Asp Ala Arg 
            580                 585                 590         


Asn Thr Gln Asp His Val Arg Ser Leu Cys Leu Leu Ala Trp His Asn 
        595                 600                 605             


Gly Lys Glu Glu Tyr Glu Lys Phe Val Ser Thr Ile Arg Ser Val Pro 
    610                 615                 620                 


Ile Gly Lys Ala Leu Ala Ile Pro Asn Phe Glu Asn Leu Arg Arg Asn 
625                 630                 635                 640 


Trp Leu Glu Leu Phe 
                645 


<210>  3
<211>  5302
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression cassette

<400>  3
ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg       60

ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag      120

acccaagctg gctagttaag ctatcaacaa gtttgtacaa aaaagcaggc tatgggctca      180

caggtgtcca cacagcgctc cggttcgcac gaaaactcta actcagctac cgagggttcc      240

actataaact atactaccat taattactat aaagattcct atgccgccac agcaggtaag      300

cagagcctta agcaggaccc agacaagttt gcaaatcctg tcaaagacat cttcactgaa      360

atggcagcgc cattaaaatc tccatctgcc gaggcatgtg gttacagcga tcgggtggca      420

caattaacta ttggcaattc taccatcact acgcaagaag cagcaaacat catagttggc      480

tatggtgagt ggccttccta ctgttcggac tctgatgcta ctgcagtgga caaaccaacg      540

cgcccagatg tttcggtgaa taggttttac acattggaca caaaattgtg ggagaaatca      600

tccaaggggt ggtactggaa attcccggat gtgttaactg aaaccggggt ctttggtcaa      660

aatgcacagt tccactacct ctatcggtca gggttctgca ttcacgtgca gtgcaatgct      720

agtaagttcc accaaggagc actcctagtc gctgtcctcc cagagtatgt cattgggaca      780

gtggcaggtg gcacagggac ggaggatagc cacccccctt ataagcagac tcaacccggt      840

gctgatggct tcgaattgca acacccgtac gtgcttgatg ctggcattcc aatatcacaa      900

ttaacagtgt gcccacatca gtggattaat ttgaggacca acaattgtgc cacaataata      960

gtgccgtaca taaacgcact accctttgat tctgccttga accattgtaa ctttggtctg     1020

ctggttgtgc ctattagccc gttagattat gaccaaggtg cgacgccagt gatccccatt     1080

actatcactt tggccccaat gtgttttgaa tttgcaggct ttagacaagc agttacgcaa     1140

gggtttccta ctgagttgaa acctggcaca aaccaatttt taaccactga cgatggcgtc     1200

tcagcaccca ttctgccaaa ctttcacccc accccgtgta tccatatacc cggtgaagtt     1260

agaaacttgc tagagctatg ccaggtggag accattttag aggtcaacaa tgtacctacg     1320

aatgccacta gcttaatgga gagactgcgc ttcccggtct cagctcaagc cgggaaaggt     1380

gagctatgtg cagtgttcag agctgaccct ggacgaagtg ggccatggca gtccaccttg     1440

ttgggccagt tgtgcgggta ctacacccaa tggtcaggat cactggaagt caccttcatg     1500

ttcaccgggt cctttatggc taccggcaag atgctcatag catacacacc accaggaggc     1560

cccttaccca aggaccgggc gaccgccatg ttgggcacgg acgtcatctg ggactttggg     1620

ctgcaatcgt ctgtcactct tgtaatacca tggatcagca acactcatta cagagcgcac     1680

gctcgagatg gtgtgtttga ctactacact acaggtttgg ttagcatatg gtaccagacg     1740

aattatgtgg ttccaattgg agcacccaat acagcctata taatagcatt ggcggcagcc     1800

cagaagaact tcaccatgaa gttgtgtaag gatgctagtg atatcctaca gacaggcact     1860

atccagggag atagggtggc agatgtgatt gagagttcta taggggacag cgtgagcaga     1920

gccctcaccc gagctctacc ggcacctacc ggccaagaca cacaggtaag cagccatcga     1980

ttagatactg gtaaaggtcc agcactccaa gccgctgaaa ttggagcatc atcaaatgct     2040

agtgatgaga gtatgattga gacacggtgt gttcttaatt cacatagcac agctgagacc     2100

actcttgata gcttcttcag cagagcagga ttagttggag agatagacct ccctcttgaa     2160

ggcacaacca acccgaatgg gtacgcaaac tgggacatag acataacagg ttacgcgcaa     2220

atgcgtagaa aggtggagct gttcacctac atgcgtttta acgcagagat cacctttgtt     2280

gcatgcaccc ctaccgggga agttgtcccg caattgctcc aatatatgtt tgtaccaccc     2340

ggagccccca agccagactc cagagaatct ctcgcatggc aaactgccac taatccctcg     2400

gtttttgtga agctgtcaga ccccccagca caggtttctg ttccattcat gtcacctgcg     2460

agcgcctatc aatggtttta tgacgggtat cccacattcg gtgaacacaa acaggagaaa     2520

gaccttgaat acggggcatg cccaaacaac atgatgggta cgttctcagt gcggactgta     2580

ggcacctcga agtccaagtg cccattggtg atcaggattt acatgaggat gaagcacgtc     2640

agggcgtgga tacctcgccc aatgcgtaac cagaactatc tattcaaagc caacccaaat     2700

tatgctggta attctattaa accaactggt gccagtcgca cagcaatgaa ttctgcatct     2760

agggcggcca attccgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct     2820

tggaataagg ccggtgtgcg tttgtctata tgtgattttc caccatattg ccgtcttttg     2880

gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt     2940

cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg     3000

aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac     3060

ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg     3120

cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct     3180

caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg     3240

atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag     3300

gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa gcttgccagc     3360

ggccgcgggc cgagcttgga cttcgcccta tctctactta ggaggaacat taggcaggtc     3420

caaaccgacc agggccactt tacaatgtta ggagtgcgag accgcttggc tgtgctcccc     3480

agacactccc aaccaggaaa gaccatctgg gttgaacaca aattagtgaa gatcgtagat     3540

gctgtggagt tagtagacga acaaggggtt aacttagagc tcacactggt aacgcttgat     3600

actaacgaaa aatttagaga catcacaaga ttcataccag aaacaattag tcctgctagt     3660

gatgccactt tagttataaa tactgaacat atgcccagta tgtttgtgcc agttggagat     3720

gtggtccagt atgggttttt gaaccttagt ggtaagccca ctcacaggac tatgatgtac     3780

aatttcccaa caaaagcagg acagtgtggt ggtgttgtga ctgccgtggg taaagtgatt     3840

gggatccaca ttggtggcaa cggtagacaa ggtttctgcg ctgccctgaa gaggggatac     3900

ttttgcagtg aacaaggtga gatccaatgg atgaagccca acaaagaaac tggcaggttg     3960

aacatcaacg gacctactcg cactaagctt gaaccaagtg tctttcacga tgtgttcgaa     4020

ggcactaaag agccagcagt gctgactagt aaagacccaa ggctggaagt tgactttgaa     4080

caggctcttt tttcaaaata cgtggggaac acgcttcatg aacccgacga gtttgtcaag     4140

gaggcggccc tacattatgc caaccaactc aagcagttag atatcaagac caccaagatg     4200

agcatggagg atgcatgtta cggcacagag aacctggaag ctatagatct tcacacaagt     4260

gcaggatatc catacagtgc actaggcatc aagaaaaagg acattttgga tccaacaact     4320

cgcgatgtca gcaagatgaa attctacatg gacaagtatg ggttggatct accgtactct     4380

acttatgtta aagatgaact tagggccatc gacaagatca agaaagggaa gtctcgtctc     4440

atagaagcga gcagtctaaa tgactcagtg tacttgagaa tgacatttgg gcacctttat     4500

gaagctttcc acgccaatcc aggtacaatc actggttcag ctgttgggtg caacccagat     4560

gtgttctgga gcaagttacc aattctactt tcaggatcgc ttttcgcgtt tgactactcg     4620

gggtatgacg ctagtctcag cccagtgtgg ttcagggcgc tggagatagt cctgcgggaa     4680

attggatact ccgaagacgc agtgtctctc atagaaggga tcaatcacac ccatcatgtg     4740

taccgcaata aaacttattg tgttcttggg ggaatgccct caggttgctc aggcacctcc     4800

attttcaact cgatgatcaa caatatcatt attagaacac tcctgattaa aacattcaaa     4860

gggatagatc taaatgaact gaacatggtg gcctacgggg atgatgtgtt ggctagttac     4920

cccttcccaa ttgactgtct ggagttggca agaacaggca aggagtatgg tctaactatg     4980

acccctgccg acaagtcacc ctgctttaat gaggttacgt gggagaatgc cactttcttg     5040

aagagaggat tcttgcctga ttatcaattc ccgtttctca tccaccctac gatgccaatg     5100

agggagattc acgaatccat tcgttggacc aaagatgcac gaagtactca agatcacgtg     5160

cgctccctct gcttattagc atggctcaac gggaaagagg agtatgaaaa atttgtgagt     5220

gcaatcagat cagttccaat tggaaaagca ttggctatac caaattatga gaatctgagg     5280

agaaattggc tcgaattgtt tt                                              5302


<210>  4
<211>  5883
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  expression cassette

<400>  4
ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg       60

ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag      120

acccaagctg gctagttaag ctatcaacaa gtttgtacaa aaaagcaggc tatgggctca      180

caggtgtcca cacagcgctc cggttcgcac gaaaactcta actcagctac cgagggttcc      240

actataaact atactaccat taattactat aaagattcct atgccgccac agcaggtaag      300

cagagcctta agcaggaccc agacaagttt gcaaatcctg tcaaagacat cttcactgaa      360

atggcagcgc cattaaaatc tccatctgcc gaggcatgtg gttacagcga tcgggtggca      420

caattaacta ttggcaattc taccatcact acgcaagaag cagcaaacat catagttggc      480

tatggtgagt ggccttccta ctgttcggac tctgatgcta ctgcagtgga caaaccaacg      540

cgcccagatg tttcggtgaa taggttttac acattggaca caaaattgtg ggagaaatca      600

tccaaggggt ggtactggaa attcccggat gtgttaactg aaaccggggt ctttggtcaa      660

aatgcacagt tccactacct ctatcggtca gggttctgca ttcacgtgca gtgcaatgct      720

agtaagttcc accaaggagc actcctagtc gctgtcctcc cagagtatgt cattgggaca      780

gtggcaggtg gcacagggac ggaggatagc cacccccctt ataagcagac tcaacccggt      840

gctgatggct tcgaattgca acacccgtac gtgcttgatg ctggcattcc aatatcacaa      900

ttaacagtgt gcccacatca gtggattaat ttgaggacca acaattgtgc cacaataata      960

gtgccgtaca taaacgcact accctttgat tctgccttga accattgtaa ctttggtctg     1020

ctggttgtgc ctattagccc gttagattat gaccaaggtg cgacgccagt gatccccatt     1080

actatcactt tggccccaat gtgttttgaa tttgcaggct ttagacaagc agttacgcaa     1140

gggtttccta ctgagttgaa acctggcaca aaccaatttt taaccactga cgatggcgtc     1200

tcagcaccca ttctgccaaa ctttcacccc accccgtgta tccatatacc cggtgaagtt     1260

agaaacttgc tagagctatg ccaggtggag accattttag aggtcaacaa tgtacctacg     1320

aatgccacta gcttaatgga gagactgcgc ttcccggtct cagctcaagc cgggaaaggt     1380

gagctatgtg cagtgttcag agctgaccct ggacgaagtg ggccatggca gtccaccttg     1440

ttgggccagt tgtgcgggta ctacacccaa tggtcaggat cactggaagt caccttcatg     1500

ttcaccgggt cctttatggc taccggcaag atgctcatag catacacacc accaggaggc     1560

cccttaccca aggaccgggc gaccgccatg ttgggcacgg acgtcatctg ggactttggg     1620

ctgcaatcgt ctgtcactct tgtaatacca tggatcagca acactcatta cagagcgcac     1680

gctcgagatg gtgtgtttga ctactacact acaggtttgg ttagcatatg gtaccagacg     1740

aattatgtgg ttccaattgg agcacccaat acagcctata taatagcatt ggcggcagcc     1800

cagaagaact tcaccatgaa gttgtgtaag gatgctagtg atatcctaca gacaggcact     1860

atccagggag atagggtggc agatgtgatt gagagttcta taggggacag cgtgagcaga     1920

gccctcaccc gagctctacc ggcacctacc ggccaagaca cacaggtaag cagccatcga     1980

ttagatactg gtaaaggtcc agcactccaa gccgctgaaa ttggagcatc atcaaatgct     2040

agtgatgaga gtatgattga gacacggtgt gttcttaatt cacatagcac agctgagacc     2100

actcttgata gcttcttcag cagagcagga ttagttggag agatagacct ccctcttgaa     2160

ggcacaacca acccgaatgg gtacgcaaac tgggacatag acataacagg ttacgcgcaa     2220

atgcgtagaa aggtggagct gttcacctac atgcgtttta acgcagagat cacctttgtt     2280

gcatgcaccc ctaccgggga agttgtcccg caattgctcc aatatatgtt tgtaccaccc     2340

ggagccccca agccagactc cagagaatct ctcgcatggc aaactgccac taatccctcg     2400

gtttttgtga agctgtcaga ccccccagca caggtttctg ttccattcat gtcacctgcg     2460

agcgcctatc aatggtttta tgacgggtat cccacattcg gtgaacacaa acaggagaaa     2520

gaccttgaat acggggcatg cccaaacaac atgatgggta cgttctcagt gcggactgta     2580

ggcacctcga agtccaagtg cccattggtg atcaggattt acatgaggat gaagcacgtc     2640

agggcgtgga tacctcgccc aatgcgtaac cagaactatc tattcaaagc caacccaaat     2700

tatgctggta attctattaa accaactggt gccagtcgca cagcaatgaa ttcgtgaggc     2760

tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga     2820

ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat     2880

gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta     2940

gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg     3000

tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact     3060

tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag     3120

agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct     3180

gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc     3240

gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa     3300

gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt tggggtcgcg     3360

ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg     3420

cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc     3480

ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt     3540

gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg     3600

cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc     3660

tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag     3720

ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag     3780

tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc     3840

tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg     3900

gttcaaagtt tttttcttcc atttcaggtg tcgtgaaaag cggccgcggg ccgagcttgg     3960

acttcgccct atctctactt aggaggaaca ttaggcaggt ccaaaccgac cagggccact     4020

ttacaatgtt aggagtgcga gaccgcttgg ctgtgctccc cagacactcc caaccaggaa     4080

agaccatctg ggttgaacac aaattagtga agatcgtaga tgctgtggag ttagtagacg     4140

aacaaggggt taacttagag ctcacactgg taacgcttga tactaacgaa aaatttagag     4200

acatcacaag attcatacca gaaacaatta gtcctgctag tgatgccact ttagttataa     4260

atactgaaca tatgcccagt atgtttgtgc cagttggaga tgtggtccag tatgggtttt     4320

tgaaccttag tggtaagccc actcacagga ctatgatgta caatttccca acaaaagcag     4380

gacagtgtgg tggtgttgtg actgccgtgg gtaaagtgat tgggatccac attggtggca     4440

acggtagaca aggtttctgc gctgccctga agaggggata cttttgcagt gaacaaggtg     4500

agatccaatg gatgaagccc aacaaagaaa ctggcaggtt gaacatcaac ggacctactc     4560

gcactaagct tgaaccaagt gtctttcacg atgtgttcga aggcactaaa gagccagcag     4620

tgctgactag taaagaccca aggctggaag ttgactttga acaggctctt ttttcaaaat     4680

acgtggggaa cacgcttcat gaacccgacg agtttgtcaa ggaggcggcc ctacattatg     4740

ccaaccaact caagcagtta gatatcaaga ccaccaagat gagcatggag gatgcatgtt     4800

acggcacaga gaacctggaa gctatagatc ttcacacaag tgcaggatat ccatacagtg     4860

cactaggcat caagaaaaag gacattttgg atccaacaac tcgcgatgtc agcaagatga     4920

aattctacat ggacaagtat gggttggatc taccgtactc tacttatgtt aaagatgaac     4980

ttagggccat cgacaagatc aagaaaggga agtctcgtct catagaagcg agcagtctaa     5040

atgactcagt gtacttgaga atgacatttg ggcaccttta tgaagctttc cacgccaatc     5100

caggtacaat cactggttca gctgttgggt gcaacccaga tgtgttctgg agcaagttac     5160

caattctact ttcaggatcg cttttcgcgt ttgactactc ggggtatgac gctagtctca     5220

gcccagtgtg gttcagggcg ctggagatag tcctgcggga aattggatac tccgaagacg     5280

cagtgtctct catagaaggg atcaatcaca cccatcatgt gtaccgcaat aaaacttatt     5340

gtgttcttgg gggaatgccc tcaggttgct caggcacctc cattttcaac tcgatgatca     5400

acaatatcat tattagaaca ctcctgatta aaacattcaa agggatagat ctaaatgaac     5460

tgaacatggt ggcctacggg gatgatgtgt tggctagtta ccccttccca attgactgtc     5520

tggagttggc aagaacaggc aaggagtatg gtctaactat gacccctgcc gacaagtcac     5580

cctgctttaa tgaggttacg tgggagaatg ccactttctt gaagagagga ttcttgcctg     5640

attatcaatt cccgtttctc atccacccta cgatgccaat gagggagatt cacgaatcca     5700

ttcgttggac caaagatgca cgaagtactc aagatcacgt gcgctccctc tgcttattag     5760

catggctcaa cgggaaagag gagtatgaaa aatttgtgag tgcaatcaga tcagttccaa     5820

ttggaaaagc attggctata ccaaattatg agaatctgag gagaaattgg ctcgaattgt     5880

ttt                                                                   5883


<210>  5
<211>  183
<212>  PRT
<213>  Enterovirus 71

<400>  5

Gly Pro Ser Leu Asp Phe Ala Leu Ser Leu Leu Arg Arg Asn Ile Arg 
1               5                   10                  15      


Gln Val Gln Thr Asp Gln Gly His Phe Thr Met Leu Gly Val Arg Asp 
            20                  25                  30          


Arg Leu Ala Val Leu Pro Arg His Ser Gln Pro Gly Lys Thr Ile Trp 
        35                  40                  45              


Val Glu His Lys Leu Val Lys Ile Val Asp Ala Val Glu Leu Val Asp 
    50                  55                  60                  


Glu Gln Gly Val Asn Leu Glu Leu Thr Leu Val Thr Leu Asp Thr Asn 
65                  70                  75                  80  


Glu Lys Phe Arg Asp Ile Thr Arg Phe Ile Pro Glu Thr Ile Ser Pro 
                85                  90                  95      


Ala Ser Asp Ala Thr Leu Val Ile Asn Thr Glu His Met Pro Ser Met 
            100                 105                 110         


Phe Val Pro Val Gly Asp Val Val Gln Tyr Gly Phe Leu Asn Leu Ser 
        115                 120                 125             


Gly Lys Pro Thr His Arg Thr Met Met Tyr Asn Phe Pro Thr Lys Ala 
    130                 135                 140                 


Gly Gln Cys Gly Gly Val Val Thr Ala Val Gly Lys Val Ile Gly Ile 
145                 150                 155                 160 


His Ile Gly Gly Asn Gly Arg Gln Gly Phe Cys Ala Ala Leu Lys Arg 
                165                 170                 175     


Gly Tyr Phe Cys Ser Glu Gln 
            180             


<210>  6
<211>  462
<212>  PRT
<213>  Enterovirus 71

<400>  6

Gly Glu Ile Gln Trp Met Lys Pro Asn Lys Glu Thr Gly Arg Leu Asn 
1               5                   10                  15      


Ile Asn Gly Pro Thr Arg Thr Lys Leu Glu Pro Ser Val Phe His Asp 
            20                  25                  30          


Val Phe Glu Gly Thr Lys Glu Pro Ala Val Leu Thr Ser Lys Asp Pro 
        35                  40                  45              


Arg Leu Glu Val Asp Phe Glu Gln Ala Leu Phe Ser Lys Tyr Val Gly 
    50                  55                  60                  


Asn Thr Leu His Glu Pro Asp Glu Phe Val Lys Glu Ala Ala Leu His 
65                  70                  75                  80  


Tyr Ala Asn Gln Leu Lys Gln Leu Asp Ile Lys Thr Thr Lys Met Ser 
                85                  90                  95      


Met Glu Asp Ala Cys Tyr Gly Thr Glu Asn Leu Glu Ala Ile Asp Leu 
            100                 105                 110         


His Thr Ser Ala Gly Tyr Pro Tyr Ser Ala Leu Gly Ile Lys Lys Lys 
        115                 120                 125             


Asp Ile Leu Asp Pro Thr Thr Arg Asp Val Ser Lys Met Lys Phe Tyr 
    130                 135                 140                 


Met Asp Lys Tyr Gly Leu Asp Leu Pro Tyr Ser Thr Tyr Val Lys Asp 
145                 150                 155                 160 


Glu Leu Arg Ala Ile Asp Lys Ile Lys Lys Gly Lys Ser Arg Leu Ile 
                165                 170                 175     


Glu Ala Ser Ser Leu Asn Asp Ser Val Tyr Leu Arg Met Thr Phe Gly 
            180                 185                 190         


His Leu Tyr Glu Ala Phe His Ala Asn Pro Gly Thr Ile Thr Gly Ser 
        195                 200                 205             


Ala Val Gly Cys Asn Pro Asp Val Phe Trp Ser Lys Leu Pro Ile Leu 
    210                 215                 220                 


Leu Ser Gly Ser Leu Phe Ala Phe Asp Tyr Ser Gly Tyr Asp Ala Ser 
225                 230                 235                 240 


Leu Ser Pro Val Trp Phe Arg Ala Leu Glu Ile Val Leu Arg Glu Ile 
                245                 250                 255     


Gly Tyr Ser Glu Asp Ala Val Ser Leu Ile Glu Gly Ile Asn His Thr 
            260                 265                 270         


His His Val Tyr Arg Asn Lys Thr Tyr Cys Val Leu Gly Gly Met Pro 
        275                 280                 285             


Ser Gly Cys Ser Gly Thr Ser Ile Phe Asn Ser Met Ile Asn Asn Ile 
    290                 295                 300                 


Ile Ile Arg Thr Leu Leu Ile Lys Thr Phe Lys Gly Ile Asp Leu Asn 
305                 310                 315                 320 


Glu Leu Asn Met Val Ala Tyr Gly Asp Asp Val Leu Ala Ser Tyr Pro 
                325                 330                 335     


Phe Pro Ile Asp Cys Leu Glu Leu Ala Arg Thr Gly Lys Glu Tyr Gly 
            340                 345                 350         


Leu Thr Met Thr Pro Ala Asp Lys Ser Pro Cys Phe Asn Glu Val Thr 
        355                 360                 365             


Trp Glu Asn Ala Thr Phe Leu Lys Arg Gly Phe Leu Pro Asp Tyr Gln 
    370                 375                 380                 


Phe Pro Phe Leu Ile His Pro Thr Met Pro Met Arg Glu Ile His Glu 
385                 390                 395                 400 


Ser Ile Arg Trp Thr Lys Asp Ala Arg Ser Thr Gln Asp His Val Arg 
                405                 410                 415     


Ser Leu Cys Leu Leu Ala Trp Leu Asn Gly Lys Glu Glu Tyr Glu Lys 
            420                 425                 430         


Phe Val Ser Ala Ile Arg Ser Val Pro Ile Gly Lys Ala Leu Ala Ile 
        435                 440                 445             


Pro Asn Tyr Glu Asn Leu Arg Arg Asn Trp Leu Glu Leu Phe 
    450                 455                 460         


<210>  7
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer F (VP1)

<400>  7
acgcgcaaat gcgtagaaag gt                                                22


<210>  8
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer R (VP1)

<400>  8
ttagtggcag tttgccatgc ga                                                22


<210>  9
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer F (b-actin)

<400>  9
accaactggg acgacatgga gaaa                                              24


<210>  10
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer R (b-actin)

<400>  10
tagcacagcc tggatagcaa cgta                                              24


<210>  11
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer F (P1)

<400>  11
atcggaattc atgggctcac aggtgtccac                                        30


<210>  12
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer R (P1)

<400>  12
cttgtcgact tagagagtgg taattgctg                                         29


<210>  13
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer F (3CD)

<400>  13
atcggaattc atggggccga gcttggac                                          28


<210>  14
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer R (3CD)

<400>  14
atcgctcgag aaacaattcg agcc                                              24


<210>  15
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer F (EF-1a)

<400>  15
atcgacgcgt gtgaggctcc ggtgccc                                           27


<210>  16
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer R (EF-1a)

<400>  16
atcgcccggg gttttcacga cacctg                                            26


