                         SEQUENCE LISTING

<110>  Massachusetts Institute of Technology
       The Governors of the University of Alberta
       President and Fellows of Harvard College
       Klapoetke, Nathan
       Boyden, Edward
       Cho, Yongku
       Chow, Brian Y
       Wong, Gane K.S.
       Cohen, Adam E
       Hochbaum, Daniel R
 
<120>  BLUE LIGHT-ACTIVATED ION CHANNEL MOLECULES AND USES THEREOF

<130>  ML01.005WO

<150>  US 61/937066
<151>  2014-02-07

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  948
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide, Mammalian-condon optimized DNA

<400>  1
atgggcggag ctcctgctcc agacgctcac agcgccccac ctggaaacga ttctgccgga       60

ggcagtgagt accatgcccc agctggatat caagtgaatc caccctacca ccccgtgcat      120

gggtatgagg aacagtgcag ctccatctac atctactatg gggccctgtg ggagcaggaa      180

acagctaggg gcttccagtg gtttgccgtg ttcctgtctg ccctgtttct ggctttctac      240

ggctggcacg cctataaggc cagcgtggga tgggaggaag tgtacgtgtg ctccgtggag      300

ctgatcaaag tgattctgga gatctatttc gagttcacca gtcctgctat gctgttcctg      360

tacggaggga acattacccc atggctgaga tatgccgaat ggctgctgac atgtcccgtg      420

atcctgattc atctgtctaa catcaccggc ctgagtgagg aatacaataa gcggacaatg      480

gctctgctgg tgtccgacct gggaactatt tgcatgggag tgacagccgc tctggccact      540

gggtgggtga agtggctgtt ttactgtatc ggcctggtgt atggaaccca gacattctac      600

aacgctggaa tcatctacgt ggagtcttac tatatcatgc ctgccggcgg ctgtaagaaa      660

ctggtgctgg ccatgactgc cgtgtactat tctagttggc tgatgtttcc cggcctgttc      720

atctttgggc ctgaaggcat gcacaccctg agcgtggctg ggtccactat tggccatacc      780

atcgccgacc tgctgtccaa gaatatttgg ggactgctgg ggcacttcct gcggatcaaa      840

attcacgagc atatcattat gtacggcgat atcaggagac cagtgagctc ccagtttctg      900

ggacgcaagg tggacgtgct ggccttcgtg acagaggaag ataaagtg                   948


<210>  2
<211>  316
<212>  PRT
<213>  Scherffelia dubia

<400>  2

Met Gly Gly Ala Pro Ala Pro Asp Ala His Ser Ala Pro Pro Gly Asn 
1               5                   10                  15      


Asp Ser Ala Gly Gly Ser Glu Tyr His Ala Pro Ala Gly Tyr Gln Val 
            20                  25                  30          


Asn Pro Pro Tyr His Pro Val His Gly Tyr Glu Glu Gln Cys Ser Ser 
        35                  40                  45              


Ile Tyr Ile Tyr Tyr Gly Ala Leu Trp Glu Gln Glu Thr Ala Arg Gly 
    50                  55                  60                  


Phe Gln Trp Phe Ala Val Phe Leu Ser Ala Leu Phe Leu Ala Phe Tyr 
65                  70                  75                  80  


Gly Trp His Ala Tyr Lys Ala Ser Val Gly Trp Glu Glu Val Tyr Val 
                85                  90                  95      


Cys Ser Val Glu Leu Ile Lys Val Ile Leu Glu Ile Tyr Phe Glu Phe 
            100                 105                 110         


Thr Ser Pro Ala Met Leu Phe Leu Tyr Gly Gly Asn Ile Thr Pro Trp 
        115                 120                 125             


Leu Arg Tyr Ala Glu Trp Leu Leu Thr Cys Pro Val Ile Leu Ile His 
    130                 135                 140                 


Leu Ser Asn Ile Thr Gly Leu Ser Glu Glu Tyr Asn Lys Arg Thr Met 
145                 150                 155                 160 


Ala Leu Leu Val Ser Asp Leu Gly Thr Ile Cys Met Gly Val Thr Ala 
                165                 170                 175     


Ala Leu Ala Thr Gly Trp Val Lys Trp Leu Phe Tyr Cys Ile Gly Leu 
            180                 185                 190         


Val Tyr Gly Thr Gln Thr Phe Tyr Asn Ala Gly Ile Ile Tyr Val Glu 
        195                 200                 205             


Ser Tyr Tyr Ile Met Pro Ala Gly Gly Cys Lys Lys Leu Val Leu Ala 
    210                 215                 220                 


Met Thr Ala Val Tyr Tyr Ser Ser Trp Leu Met Phe Pro Gly Leu Phe 
225                 230                 235                 240 


Ile Phe Gly Pro Glu Gly Met His Thr Leu Ser Val Ala Gly Ser Thr 
                245                 250                 255     


Ile Gly His Thr Ile Ala Asp Leu Leu Ser Lys Asn Ile Trp Gly Leu 
            260                 265                 270         


Leu Gly His Phe Leu Arg Ile Lys Ile His Glu His Ile Ile Met Tyr 
        275                 280                 285             


Gly Asp Ile Arg Arg Pro Val Ser Ser Gln Phe Leu Gly Arg Lys Val 
    290                 295                 300                 


Asp Val Leu Ala Phe Val Thr Glu Glu Asp Lys Val 
305                 310                 315     


<210>  3
<211>  864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide, Mammalian-condon optimized DNA

<400>  3
atgctgggaa acggcagcgc cattgtgcct atcgaccagt gcttttgcct ggcttggacc       60

gacagcctgg gaagcgatac agagcagctg gtggccaaca tcctccagtg gttcgccttc      120

ggcttcagca tcctgatcct gatgttctac gcctaccaga cttggagagc cacttgcggt      180

tgggaggagg tctacgtctg ttgcgtcgag ctgaccaagg tcatcatcga gttcttccac      240

gagttcgacg accccagcat gctgtacctg gctaacggac accgagtcca gtggctgaga      300

tacgcagagt ggctgctgac ttgtcccgtc atcctgatcc acctgagcaa cctgaccggc      360

ctgaaggacg actacagcaa gcggaccatg aggctgctgg tgtcagacgt gggaaccatc      420

gtgtggggag ctacaagcgc catgagcaca ggctacgtca aggtcatctt cttcgtgctg      480

ggttgcatct acggcgccaa caccttcttc cacgccgcca aggtgtatat cgagagctac      540

cacgtggtgc caaagggcag acctagaacc gtcgtgcgga tcatggcttg gctgttcttc      600

ctgtcttggg gcatgttccc cgtgctgttc gtcgtgggac cagaaggatt cgacgccatc      660

agcgtgtacg gctctaccat tggccacacc atcatcgacc tcatgagcaa gaattgttgg      720

ggcctgctgg gacactatct gagagtgctg atccaccagc acatcatcat ctacggcgac      780

atccggaaga agaccaagat caacgtggcc ggcgaggaga tggaagtgga gaccatggtg      840

gaccaggagg acgaggagac agtg                                             864


<210>  4
<211>  288
<212>  PRT
<213>  Chloromonas oogama

<400>  4

Met Leu Gly Asn Gly Ser Ala Ile Val Pro Ile Asp Gln Cys Phe Cys 
1               5                   10                  15      


Leu Ala Trp Thr Asp Ser Leu Gly Ser Asp Thr Glu Gln Leu Val Ala 
            20                  25                  30          


Asn Ile Leu Gln Trp Phe Ala Phe Gly Phe Ser Ile Leu Ile Leu Met 
        35                  40                  45              


Phe Tyr Ala Tyr Gln Thr Trp Arg Ala Thr Cys Gly Trp Glu Glu Val 
    50                  55                  60                  


Tyr Val Cys Cys Val Glu Leu Thr Lys Val Ile Ile Glu Phe Phe His 
65                  70                  75                  80  


Glu Phe Asp Asp Pro Ser Met Leu Tyr Leu Ala Asn Gly His Arg Val 
                85                  90                  95      


Gln Trp Leu Arg Tyr Ala Glu Trp Leu Leu Thr Cys Pro Val Ile Leu 
            100                 105                 110         


Ile His Leu Ser Asn Leu Thr Gly Leu Lys Asp Asp Tyr Ser Lys Arg 
        115                 120                 125             


Thr Met Arg Leu Leu Val Ser Asp Val Gly Thr Ile Val Trp Gly Ala 
    130                 135                 140                 


Thr Ser Ala Met Ser Thr Gly Tyr Val Lys Val Ile Phe Phe Val Leu 
145                 150                 155                 160 


Gly Cys Ile Tyr Gly Ala Asn Thr Phe Phe His Ala Ala Lys Val Tyr 
                165                 170                 175     


Ile Glu Ser Tyr His Val Val Pro Lys Gly Arg Pro Arg Thr Val Val 
            180                 185                 190         


Arg Ile Met Ala Trp Leu Phe Phe Leu Ser Trp Gly Met Phe Pro Val 
        195                 200                 205             


Leu Phe Val Val Gly Pro Glu Gly Phe Asp Ala Ile Ser Val Tyr Gly 
    210                 215                 220                 


Ser Thr Ile Gly His Thr Ile Ile Asp Leu Met Ser Lys Asn Cys Trp 
225                 230                 235                 240 


Gly Leu Leu Gly His Tyr Leu Arg Val Leu Ile His Gln His Ile Ile 
                245                 250                 255     


Ile Tyr Gly Asp Ile Arg Lys Lys Thr Lys Ile Asn Val Ala Gly Glu 
            260                 265                 270         


Glu Met Glu Val Glu Thr Met Val Asp Gln Glu Asp Glu Glu Thr Val 
        275                 280                 285             


<210>  5
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide, mammalian-codon optimized DNA

<400>  5
atggactatg gcggcgcttt gtctgccgtc ggacgcgaac ttttgttcgt tactaatcct       60

gtggtggtga acgggtccgt cctggtccct gaggatcaat gttactgtgc cggatggatt      120

gaatctcgcg gcacgaacgg cgctcagacc gcgtcaaatg tcctgcagtg gcttgcagca      180

ggattcagca ttttgctgct gatgttctat gcctaccaaa cctggaaatc tacatgcggc      240

tgggaggaga tctatgtgtg cgccattgaa atggttaagg tgattctcga gttctttttt      300

gagtttaaga atccctctat gctctacctt gccacaggac accgggtgca gtggctgcgc      360

tatgcagagt ggctgctcac ttgtcctgtc atccttatcc acctgagcaa cctcaccggc      420

ctgagcaacg actacagcag gagaaccatg ggactccttg tctcagacat cgggactatc      480

gtgtgggggg ctaccagcgc catggcaacc ggctatgtta aagtcatctt cttttgtctt      540

ggattgtgct atggcgcgaa cacatttttt cacgccgcca aagcatatat cgagggttat      600

catactgtgc caaagggtcg gtgccgccag gtcgtgaccg gcatggcatg gctgtttttc      660

gtgagctggg gtatgttccc aattctcttc attttggggc ccgaaggttt tggcgtcctg      720

agcgtctatg gctccaccgt aggtcacacg attattgatc tgatgagtaa aaattgttgg      780

gggttgttgg gacactacct gcgcgtcctg atccacgagc acatattgat tcacggagat      840

atccgcaaaa ccaccaaact gaacatcggc ggaacggaga tcgaggtcga gactctcgtc      900

gaagacgaag ccgaggccgg agccgtg                                          927


<210>  6
<211>  309
<212>  PRT
<213>  Chlamydomonas reinhardtii

<400>  6

Met Asp Tyr Gly Gly Ala Leu Ser Ala Val Gly Arg Glu Leu Leu Phe 
1               5                   10                  15      


Val Thr Asn Pro Val Val Val Asn Gly Ser Val Leu Val Pro Glu Asp 
            20                  25                  30          


Gln Cys Tyr Cys Ala Gly Trp Ile Glu Ser Arg Gly Thr Asn Gly Ala 
        35                  40                  45              


Gln Thr Ala Ser Asn Val Leu Gln Trp Leu Ala Ala Gly Phe Ser Ile 
    50                  55                  60                  


Leu Leu Leu Met Phe Tyr Ala Tyr Gln Thr Trp Lys Ser Thr Cys Gly 
65                  70                  75                  80  


Trp Glu Glu Ile Tyr Val Cys Ala Ile Glu Met Val Lys Val Ile Leu 
                85                  90                  95      


Glu Phe Phe Phe Glu Phe Lys Asn Pro Ser Met Leu Tyr Leu Ala Thr 
            100                 105                 110         


Gly His Arg Val Gln Trp Leu Arg Tyr Ala Glu Trp Leu Leu Thr Cys 
        115                 120                 125             


Pro Val Ile Leu Ile His Leu Ser Asn Leu Thr Gly Leu Ser Asn Asp 
    130                 135                 140                 


Tyr Ser Arg Arg Thr Met Gly Leu Leu Val Ser Asp Ile Gly Thr Ile 
145                 150                 155                 160 


Val Trp Gly Ala Thr Ser Ala Met Ala Thr Gly Tyr Val Lys Val Ile 
                165                 170                 175     


Phe Phe Cys Leu Gly Leu Cys Tyr Gly Ala Asn Thr Phe Phe His Ala 
            180                 185                 190         


Ala Lys Ala Tyr Ile Glu Gly Tyr His Thr Val Pro Lys Gly Arg Cys 
        195                 200                 205             


Arg Gln Val Val Thr Gly Met Ala Trp Leu Phe Phe Val Ser Trp Gly 
    210                 215                 220                 


Met Phe Pro Ile Leu Phe Ile Leu Gly Pro Glu Gly Phe Gly Val Leu 
225                 230                 235                 240 


Ser Val Tyr Gly Ser Thr Val Gly His Thr Ile Ile Asp Leu Met Ser 
                245                 250                 255     


Lys Asn Cys Trp Gly Leu Leu Gly His Tyr Leu Arg Val Leu Ile His 
            260                 265                 270         


Glu His Ile Leu Ile His Gly Asp Ile Arg Lys Thr Thr Lys Leu Asn 
        275                 280                 285             


Ile Gly Gly Thr Glu Ile Glu Val Glu Thr Leu Val Glu Asp Glu Ala 
    290                 295                 300                 


Glu Ala Gly Ala Val 
305                 


<210>  7
<211>  316
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  7

Met Gly Gly Ala Pro Ala Pro Asp Ala His Ser Ala Pro Pro Gly Asn 
1               5                   10                  15      


Asp Ser Ala Gly Gly Ser Glu Tyr His Ala Pro Ala Gly Tyr Gln Val 
            20                  25                  30          


Asn Pro Pro Tyr His Pro Val His Gly Tyr Glu Glu Gln Cys Ser Ser 
        35                  40                  45              


Ile Tyr Ile Tyr Tyr Gly Ala Leu Trp Glu Gln Glu Thr Ala Arg Gly 
    50                  55                  60                  


Phe Gln Trp Phe Ala Val Phe Leu Ser Ala Leu Phe Leu Ala Phe Tyr 
65                  70                  75                  80  


Gly Trp His Ala Tyr Lys Ala Ser Val Gly Trp Glu Glu Val Tyr Val 
                85                  90                  95      


Cys Ser Val Glu Leu Ile Lys Val Ile Leu Glu Ile Tyr Phe Glu Phe 
            100                 105                 110         


Thr Ser Pro Ala Met Leu Phe Leu Tyr Gly Gly Asn Ile Thr Pro Trp 
        115                 120                 125             


Leu Arg Tyr Ala Glu Trp Leu Leu Thr Cys Pro Val Ile Leu Ile His 
    130                 135                 140                 


Leu Ser Asn Ile Thr Gly Leu Ser Glu Ala Tyr Asn Lys Arg Thr Met 
145                 150                 155                 160 


Ala Leu Leu Val Ser Asp Leu Gly Thr Ile Cys Met Gly Val Thr Ala 
                165                 170                 175     


Ala Leu Ala Thr Gly Trp Val Lys Trp Leu Phe Tyr Cys Ile Gly Leu 
            180                 185                 190         


Val Tyr Gly Thr Gln Thr Phe Tyr Asn Ala Gly Ile Ile Tyr Val Glu 
        195                 200                 205             


Ser Tyr Tyr Ile Met Pro Ala Gly Gly Cys Lys Lys Leu Val Leu Ala 
    210                 215                 220                 


Met Thr Ala Val Tyr Tyr Ser Ser Trp Leu Met Phe Pro Gly Leu Phe 
225                 230                 235                 240 


Ile Phe Gly Pro Glu Gly Met His Thr Leu Ser Val Ala Gly Ser Thr 
                245                 250                 255     


Ile Gly His Thr Ile Ala Asp Leu Leu Ser Lys Asn Ile Trp Gly Leu 
            260                 265                 270         


Leu Gly His Phe Leu Arg Ile Lys Ile His Glu His Ile Ile Met Tyr 
        275                 280                 285             


Gly Asp Ile Arg Arg Pro Val Ser Ser Gln Phe Leu Gly Arg Lys Val 
    290                 295                 300                 


Asp Val Leu Ala Phe Val Thr Glu Glu Asp Lys Val 
305                 310                 315     


<210>  8
<211>  288
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  8

Met Leu Gly Asn Gly Ser Ala Ile Val Pro Ile Asp Gln Cys Phe Cys 
1               5                   10                  15      


Leu Ala Trp Thr Asp Ser Leu Gly Ser Asp Thr Glu Gln Leu Val Ala 
            20                  25                  30          


Asn Ile Leu Gln Trp Phe Ala Phe Gly Phe Ser Ile Leu Ile Leu Met 
        35                  40                  45              


Phe Tyr Ala Tyr Gln Thr Trp Arg Ala Thr Cys Gly Trp Glu Glu Val 
    50                  55                  60                  


Tyr Val Cys Cys Val Glu Leu Thr Lys Val Ile Ile Glu Phe Phe His 
65                  70                  75                  80  


Glu Phe Asp Asp Pro Ser Met Leu Tyr Leu Ala Asn Gly His Arg Val 
                85                  90                  95      


Gln Trp Leu Arg Tyr Ala Glu Trp Leu Leu Thr Cys Pro Val Ile Leu 
            100                 105                 110         


Ile His Leu Ser Asn Leu Thr Gly Leu Lys Asp Ala Tyr Ser Lys Arg 
        115                 120                 125             


Thr Met Arg Leu Leu Val Ser Asp Val Gly Thr Ile Val Trp Gly Ala 
    130                 135                 140                 


Thr Ser Ala Met Ser Thr Gly Tyr Val Lys Val Ile Phe Phe Val Leu 
145                 150                 155                 160 


Gly Cys Ile Tyr Gly Ala Asn Thr Phe Phe His Ala Ala Lys Val Tyr 
                165                 170                 175     


Ile Glu Ser Tyr His Val Val Pro Lys Gly Arg Pro Arg Thr Val Val 
            180                 185                 190         


Arg Ile Met Ala Trp Leu Phe Phe Leu Ser Trp Gly Met Phe Pro Val 
        195                 200                 205             


Leu Phe Val Val Gly Pro Glu Gly Phe Asp Ala Ile Ser Val Tyr Gly 
    210                 215                 220                 


Ser Thr Ile Gly His Thr Ile Ile Asp Leu Met Ser Lys Asn Cys Trp 
225                 230                 235                 240 


Gly Leu Leu Gly His Tyr Leu Arg Val Leu Ile His Gln His Ile Ile 
                245                 250                 255     


Ile Tyr Gly Asp Ile Arg Lys Lys Thr Lys Ile Asn Val Ala Gly Glu 
            260                 265                 270         


Glu Met Glu Val Glu Thr Met Val Asp Gln Glu Asp Glu Glu Thr Val 
        275                 280                 285             


<210>  9
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic Oligonucleotide

<400>  9
ttctgctacg agaatgaagt g                                                 21


<210>  10
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  10

Phe Cys Tyr Glu Asn Glu Val 
1               5           


<210>  11
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic oligonucleotide

<400>  11
aaatccagaa ttacttctga aggggagtat atccctctgg atcaaataga catcaatgt        59


<210>  12
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic polypeptide

<400>  12

Lys Ser Arg Ile Thr Ser Glu Gly Glu Tyr Ile Pro Leu Asp Gln Ile 
1               5                   10                  15      


Asp Ile Asn Val 
            20  


