                         SEQUENCE LISTING

<110>  Apogenix GmbH
 
<120>  Diagnostic anti CD95L antibody

<130>  54306P WO-1

<150>  PCT/EP2014/058746
<151>  2014-04-29

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  6
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR-H1

<400>  1

Ser Ser Ala Trp Ile Cys 
1               5       


<210>  2
<211>  18
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR-H2

<400>  2

Cys Ile Phe Thr Gly Asn Ser Asp Ile Thr Ile Tyr Ala Asn Trp Ala 
1               5                   10                  15      


Lys Gly 
        


<210>  3
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR-H3

<400>  3

Asn Leu Ala Ala Thr Lys Leu 
1               5           


<210>  4
<211>  11
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR-L1

<400>  4

Gln Ala Ser Gln Ser Ile Thr Asp Gln Leu Ser 
1               5                   10      


<210>  5
<211>  7
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR-L2

<400>  5

Arg Ala Ser Thr Leu Ala Ser 
1               5           


<210>  6
<211>  13
<212>  PRT
<213>  artificial sequence

<220>
<223>  CDR-L3

<400>  6

Leu Gly Val His Gly Tyr Ser Ser Asp Asp Ala Ile Ser 
1               5                   10              


<210>  7
<211>  117
<212>  PRT
<213>  artificial sequence

<220>
<223>  vH


<220>
<221>  MISC_FEATURE
<222>  (1)..(30)
<223>  HFR1

<220>
<221>  MISC_FEATURE
<222>  (31)..(36)
<223>  CDR-H1

<220>
<221>  MISC_FEATURE
<222>  (37)..(50)
<223>  HFR-2

<220>
<221>  MISC_FEATURE
<222>  (51)..(68)
<223>  CDR-H2

<220>
<221>  MISC_FEATURE
<222>  (69)..(99)
<223>  HFR3

<220>
<221>  MISC_FEATURE
<222>  (100)..(106)
<223>  CDR-H3

<220>
<221>  MISC_FEATURE
<222>  (107)..(117)
<223>  HFR-4

<400>  7

Gln Glu Gln Leu Glu Glu Ser Gly Gly Asp Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Leu Thr Leu Thr Cys Thr Ala Ser Gly Phe Ser Phe Ser Ser Ser 
            20                  25                  30          


Ala Trp Ile Cys Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp 
        35                  40                  45              


Ile Ala Cys Ile Phe Thr Gly Asn Ser Asp Ile Thr Ile Tyr Ala Asn 
    50                  55                  60                  


Trp Ala Lys Gly Arg Ser Thr Ile Ser Arg Thr Ser Ser Thr Thr Val 
65                  70                  75                  80  


Thr Leu Gln Val Thr Ser Leu Thr Ala Ala Asp Thr Ala Thr Tyr Phe 
                85                  90                  95      


Cys Ala Arg Asn Leu Ala Ala Thr Lys Leu Trp Gly Pro Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser 
        115         


<210>  8
<211>  111
<212>  PRT
<213>  artificial sequence

<220>
<223>  vL


<220>
<221>  MISC_FEATURE
<222>  (1)..(22)
<223>  LFR1

<220>
<221>  MISC_FEATURE
<222>  (23)..(33)
<223>  CDR-L1

<220>
<221>  MISC_FEATURE
<222>  (34)..(48)
<223>  LFR-2

<220>
<221>  MISC_FEATURE
<222>  (49)..(55)
<223>  CDR-L2

<220>
<221>  MISC_FEATURE
<222>  (56)..(87)
<223>  LFR3

<220>
<221>  MISC_FEATURE
<222>  (88)..(100)
<223>  CDR-L3

<220>
<221>  MISC_FEATURE
<222>  (101)..(111)
<223>  LFR4

<400>  8

Leu Val Met Thr Gln Thr Pro Ala Ser Val Ser Ala Ala Val Gly Gly 
1               5                   10                  15      


Thr Val Thr Ile Ser Cys Gln Ala Ser Gln Ser Ile Thr Asp Gln Leu 
            20                  25                  30          


Ser Trp Tyr Gln His Gln Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr 
        35                  40                  45              


Arg Ala Ser Thr Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Glu Thr Glu Phe Ser Leu Thr Ile Ser Gly Leu Gln Cys Ala 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys Leu Gly Val His Gly Tyr Ser Ser Asp 
                85                  90                  95      


Asp Ala Ile Ser Phe Gly Gly Gly Thr Glu Val Val Val Lys Gly 
            100                 105                 110     


<210>  9
<211>  440
<212>  PRT
<213>  artificial sequence

<220>
<223>  mature peptide: heavy chain


<220>
<221>  DOMAIN
<222>  (1)..(117)
<223>  vH

<400>  9

Gln Glu Gln Leu Glu Glu Ser Gly Gly Asp Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Leu Thr Leu Thr Cys Thr Ala Ser Gly Phe Ser Phe Ser Ser Ser 
            20                  25                  30          


Ala Trp Ile Cys Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp 
        35                  40                  45              


Ile Ala Cys Ile Phe Thr Gly Asn Ser Asp Ile Thr Ile Tyr Ala Asn 
    50                  55                  60                  


Trp Ala Lys Gly Arg Ser Thr Ile Ser Arg Thr Ser Ser Thr Thr Val 
65                  70                  75                  80  


Thr Leu Gln Val Thr Ser Leu Thr Ala Ala Asp Thr Ala Thr Tyr Phe 
                85                  90                  95      


Cys Ala Arg Asn Leu Ala Ala Thr Lys Leu Trp Gly Pro Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ser Gly Gln Pro Lys Ala Pro Ser Val Phe Pro Leu 
        115                 120                 125             


Ala Pro Cys Cys Gly Asp Thr Pro Ser Ser Thr Val Thr Leu Gly Cys 
    130                 135                 140                 


Leu Val Lys Gly Tyr Leu Pro Glu Pro Val Thr Val Thr Trp Asn Ser 
145                 150                 155                 160 


Gly Thr Leu Thr Asn Gly Val Arg Thr Phe Pro Ser Val Arg Gln Ser 
                165                 170                 175     


Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Ser Val Thr Ser Ser Ser 
            180                 185                 190         


Gln Pro Val Thr Cys Asn Val Ala His Pro Ala Thr Asn Thr Lys Val 
        195                 200                 205             


Asp Lys Thr Val Ala Pro Ser Thr Cys Ser Lys Pro Thr Cys Pro Pro 
    210                 215                 220                 


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro 
225                 230                 235                 240 


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
                245                 250                 255     


Val Asp Val Ser Gln Asp Asp Pro Glu Val Gln Phe Thr Trp Tyr Ile 
            260                 265                 270         


Asn Asn Glu Gln Val Arg Thr Ala Arg Pro Pro Leu Arg Glu Gln Gln 
        275                 280                 285             


Phe Asn Ser Thr Ile Arg Val Val Ser Thr Leu Pro Ile Ala His Gln 
    290                 295                 300                 


Asp Trp Leu Arg Gly Lys Glu Phe Lys Cys Lys Val His Asn Lys Ala 
305                 310                 315                 320 


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Arg Gly Gln Pro 
                325                 330                 335     


Leu Glu Pro Lys Val Tyr Thr Met Gly Pro Pro Arg Glu Glu Leu Ser 
            340                 345                 350         


Ser Arg Ser Val Ser Leu Thr Cys Met Ile Asn Gly Phe Tyr Pro Ser 
        355                 360                 365             


Asp Ile Ser Val Glu Trp Glu Lys Asn Gly Lys Ala Glu Asp Asn Tyr 
    370                 375                 380                 


Lys Thr Thr Pro Ala Val Leu Asp Ser Asp Gly Ser Tyr Phe Leu Tyr 
385                 390                 395                 400 


Ser Lys Leu Ser Val Pro Thr Ser Glu Trp Gln Arg Gly Asp Val Phe 
                405                 410                 415     


Thr Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
            420                 425                 430         


Ser Ile Ser Arg Ser Pro Gly Lys 
        435                 440 


<210>  10
<211>  214
<212>  PRT
<213>  artificial sequence

<220>
<223>  mature peptide: light chain


<220>
<221>  DOMAIN
<222>  (1)..(111)
<223>  vL

<400>  10

Leu Val Met Thr Gln Thr Pro Ala Ser Val Ser Ala Ala Val Gly Gly 
1               5                   10                  15      


Thr Val Thr Ile Ser Cys Gln Ala Ser Gln Ser Ile Thr Asp Gln Leu 
            20                  25                  30          


Ser Trp Tyr Gln His Gln Pro Gly Gln Pro Pro Lys Leu Leu Ile Tyr 
        35                  40                  45              


Arg Ala Ser Thr Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Glu Thr Glu Phe Ser Leu Thr Ile Ser Gly Leu Gln Cys Ala 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys Leu Gly Val His Gly Tyr Ser Ser Asp 
                85                  90                  95      


Asp Ala Ile Ser Phe Gly Gly Gly Thr Glu Val Val Val Lys Gly Asp 
            100                 105                 110         


Pro Val Ala Pro Thr Val Leu Ile Phe Pro Pro Ala Ala Asp Gln Val 
        115                 120                 125             


Ala Thr Gly Thr Val Thr Ile Val Cys Val Ala Asn Lys Tyr Phe Pro 
    130                 135                 140                 


Asp Val Thr Val Thr Trp Glu Val Asp Gly Thr Thr Gln Thr Thr Gly 
145                 150                 155                 160 


Ile Glu Asn Ser Lys Thr Pro Gln Asn Ser Ala Asp Cys Thr Tyr Asn 
                165                 170                 175     


Leu Ser Ser Thr Leu Thr Leu Thr Ser Thr Gln Tyr Asn Ser His Lys 
            180                 185                 190         


Glu Tyr Thr Cys Lys Val Thr Gln Gly Thr Thr Ser Val Val Gln Ser 
        195                 200                 205             


Phe Asn Arg Gly Asp Cys 
    210                 


<210>  11
<211>  1380
<212>  DNA
<213>  artificial sequence

<220>
<223>  heavy chain

<400>  11
atggagactg ggctgcgctg gcttctcctg gtcgctgtgc tcaaaggtgt ccagtgtcag       60

gagcagctgg aggagtccgg gggagacctg gtcaagcctg gggcatccct gacactcacc      120

tgcacagcct ctggattctc cttcagtagc agcgcctgga tatgctgggt ccgccaggct      180

ccagggaagg ggctggagtg gatcgcatgc attttcactg gaaatagtga tatcaccatt      240

tacgcgaact gggcgaaagg ccgatccacc atctccagaa cctcgtcgac cacggtgact      300

ctgcaagtga ccagtctgac agccgcggac acggccactt atttctgtgc gagaaattta      360

gctgctacta aattgtgggg ccccggcacc ctggtcaccg tctcctcagg gcaacctaag      420

gctccatcag tcttcccact ggccccctgc tgcggggaca cacccagctc cacggtgacc      480

ctgggctgcc tggtcaaagg gtacctcccg gagccagtga ccgtgacctg gaactcgggc      540

accctcacca atggggtacg caccttcccg tccgtccggc agtcctcagg cctctactcg      600

ctgagcagcg tggtgagcgt gacctcaagc agccagcccg tcacctgcaa cgtggcccac      660

ccagccacca acaccaaagt ggacaagacc gttgcgccct cgacatgcag caagcccacg      720

tgcccacccc ctgaactcct ggggggaccg tctgtcttca tcttcccccc aaaacccaag      780

gacaccctca tgatctcacg cacccccgag gtcacatgcg tggtggtgga cgtgagccag      840

gatgaccccg aggtgcagtt cacatggtac ataaacaacg agcaggtgcg caccgcccgg      900

ccgccgctac gggagcagca gttcaacagc acgatccgcg tggtcagcac cctccccatc      960

gcgcaccagg actggctgag gggcaaggag ttcaagtgca aagtccacaa caaggcactc     1020

ccggccccca tcgagaaaac catctccaaa gccagagggc agcccctgga gccgaaggtc     1080

tacaccatgg gccctccccg ggaggagctg agcagcaggt cggtcagcct gacctgcatg     1140

atcaacggct tctacccttc cgacatctcg gtggagtggg agaagaacgg gaaggcagag     1200

gacaactaca agaccacgcc ggccgtgctg gacagcgacg gctcctactt cctctacagc     1260

aagctctcag tgcccacgag tgagtggcag cggggcgacg tcttcacctg ctccgtgatg     1320

cacgaggcct tgcacaacca ctacacgcag aagtccatct cccgctctcc gggtaaatga     1380


<210>  12
<211>  714
<212>  DNA
<213>  artificial sequence

<220>
<223>  light chain

<400>  12
atggacacga gggcccccac tcagctgctg gggctcctgc tgctctggct cccaggtgcc       60

acatttgccc tggtgatgac ccagactcca gcctccgtgt ctgccgctgt gggaggcaca      120

gtcaccatca gttgccaggc cagtcagagc attaccgatc aactatcctg gtatcagcac      180

caaccagggc agcctcccaa gctcctgatc tacagggcat ccactctggc atctggggtc      240

ccatcgcggt tcagcggcag tggatctgag acagaattct ctctcaccat cagcggcttg      300

cagtgtgccg atgctgccac ttactactgt ctaggtgttc atggttatag tagtgatgat      360

gctatttctt tcggcggagg gaccgaggtg gtggtcaaag gtgatccagt tgcacctact      420

gtcctcatct tcccaccagc tgctgatcag gtggcaactg gaacagtcac catcgtgtgt      480

gtggcgaata aatactttcc cgatgtcacc gtcacctggg aggtggatgg caccacccaa      540

acaactggca tcgagaacag taaaacaccg cagaattctg cagattgtac ctacaacctc      600

agcagcactc tgacactgac cagcacacag tacaacagcc acaaagagta cacctgcaag      660

gtgacccagg gcacgacctc agtcgtccag agcttcaata ggggtgactg ttag            714


<210>  13
<211>  400
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant fusion protein consisting of human CD95 extracellular
       domain with human IgG1 FC-part to its C-terminus


<220>
<221>  SIGNAL
<222>  (1)..(16)
<223>  Variable cleavage sites

<220>
<221>  SIGNAL
<222>  (1)..(20)
<223>  Variable cleavage sites

<220>
<221>  SIGNAL
<222>  (1)..(25)
<223>  Variable cleavage sites

<220>
<221>  DOMAIN
<222>  (26)..(172)
<223>  Human CD95 extracellular domain

<220>
<221>  MOD_RES
<222>  (26)..(26)
<223>  PYRROLIDONE CARBOXYLIC ACID

<220>
<221>  DISULFID
<222>  (59)..(73)

<220>
<221>  DISULFID
<222>  (63)..(82)

<220>
<221>  DISULFID
<222>  (85)..(101)

<220>
<221>  DISULFID
<222>  (104)..(119)

<220>
<221>  DISULFID
<222>  (107)..(127)

<220>
<221>  CARBOHYD
<222>  (118)..(118)
<223>  N-linked Glycosylation at Position N118

<220>
<221>  DISULFID
<222>  (129)..(143)

<220>
<221>  DISULFID
<222>  (135)..(140)

<220>
<221>  CARBOHYD
<222>  (136)..(136)
<223>  N-linked Glycosylation at Position N136

<220>
<221>  DISULFID
<222>  (146)..(157)

<220>
<221>  DISULFID
<222>  (149)..(165)

<220>
<221>  DOMAIN
<222>  (172)..(400)
<223>  Human IgG1-FC domain (one amino acid overlap with CD95 domain)

<220>
<221>  DISULFID
<222>  (173)..(173)
<223>  Interchain cystine forming residue.

<220>
<221>  DISULFID
<222>  (179)..(179)
<223>  Interchain cystine forming residue.

<220>
<221>  DISULFID
<222>  (182)..(182)
<223>  Interchain cystine forming residue

<220>
<221>  DISULFID
<222>  (214)..(274)

<220>
<221>  CARBOHYD
<222>  (250)..(250)
<223>  N-linked Glycosylation at Position N250

<220>
<221>  DISULFID
<222>  (320)..(378)

<400>  13

Met Val Gly Ile Trp Thr Leu Leu Pro Leu Val Leu Thr Ser Val Ala 
1               5                   10                  15      


Arg Leu Ser Ser Lys Ser Val Asn Ala Gln Val Thr Asp Ile Asn Ser 
            20                  25                  30          


Lys Gly Leu Glu Leu Arg Lys Thr Val Thr Thr Val Glu Thr Gln Asn 
        35                  40                  45              


Leu Glu Gly Leu His His Asp Gly Gln Phe Cys His Lys Pro Cys Pro 
    50                  55                  60                  


Pro Gly Glu Arg Lys Ala Arg Asp Cys Thr Val Asn Gly Asp Glu Pro 
65                  70                  75                  80  


Asp Cys Val Pro Cys Gln Glu Gly Lys Glu Tyr Thr Asp Lys Ala His 
                85                  90                  95      


Phe Ser Ser Lys Cys Arg Arg Cys Arg Leu Cys Asp Glu Gly His Gly 
            100                 105                 110         


Leu Glu Val Glu Ile Asn Cys Thr Arg Thr Gln Asn Thr Lys Cys Arg 
        115                 120                 125             


Cys Lys Pro Asn Phe Phe Cys Asn Ser Thr Val Cys Glu His Cys Asp 
    130                 135                 140                 


Pro Cys Thr Lys Cys Glu His Gly Ile Ile Lys Glu Cys Thr Leu Thr 
145                 150                 155                 160 


Ser Asn Thr Lys Cys Lys Glu Glu Gly Ser Arg Ser Cys Asp Lys Thr 
                165                 170                 175     


His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser 
            180                 185                 190         


Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 
        195                 200                 205             


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 
    210                 215                 220                 


Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
225                 230                 235                 240 


Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 
                245                 250                 255     


Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
            260                 265                 270         


Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr 
        275                 280                 285             


Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
    290                 295                 300                 


Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys 
305                 310                 315                 320 


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
                325                 330                 335     


Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 
            340                 345                 350         


Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 
        355                 360                 365             


Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
    370                 375                 380                 


Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
385                 390                 395                 400 


<210>  14
<211>  281
<212>  PRT
<213>  Homo sapiens


<220>
<221>  MISC_FEATURE
<223>  human CD95L

<400>  14

Met Gln Gln Pro Phe Asn Tyr Pro Tyr Pro Gln Ile Tyr Trp Val Asp 
1               5                   10                  15      


Ser Ser Ala Ser Ser Pro Trp Ala Pro Pro Gly Thr Val Leu Pro Cys 
            20                  25                  30          


Pro Thr Ser Val Pro Arg Arg Pro Gly Gln Arg Arg Pro Pro Pro Pro 
        35                  40                  45              


Pro Pro Pro Pro Pro Leu Pro Pro Pro Pro Pro Pro Pro Pro Leu Pro 
    50                  55                  60                  


Pro Leu Pro Leu Pro Pro Leu Lys Lys Arg Gly Asn His Ser Thr Gly 
65                  70                  75                  80  


Leu Cys Leu Leu Val Met Phe Phe Met Val Leu Val Ala Leu Val Gly 
                85                  90                  95      


Leu Gly Leu Gly Met Phe Gln Leu Phe His Leu Gln Lys Glu Leu Ala 
            100                 105                 110         


Glu Leu Arg Glu Ser Thr Ser Gln Met His Thr Ala Ser Ser Leu Glu 
        115                 120                 125             


Lys Gln Ile Gly His Pro Ser Pro Pro Pro Glu Lys Lys Glu Leu Arg 
    130                 135                 140                 


Lys Val Ala His Leu Thr Gly Lys Ser Asn Ser Arg Ser Met Pro Leu 
145                 150                 155                 160 


Glu Trp Glu Asp Thr Tyr Gly Ile Val Leu Leu Ser Gly Val Lys Tyr 
                165                 170                 175     


Lys Lys Gly Gly Leu Val Ile Asn Glu Thr Gly Leu Tyr Phe Val Tyr 
            180                 185                 190         


Ser Lys Val Tyr Phe Arg Gly Gln Ser Cys Asn Asn Leu Pro Leu Ser 
        195                 200                 205             


His Lys Val Tyr Met Arg Asn Ser Lys Tyr Pro Gln Asp Leu Val Met 
    210                 215                 220                 


Met Glu Gly Lys Met Met Ser Tyr Cys Thr Thr Gly Gln Met Trp Ala 
225                 230                 235                 240 


Arg Ser Ser Tyr Leu Gly Ala Val Phe Asn Leu Thr Ser Ala Asp His 
                245                 250                 255     


Leu Tyr Val Asn Val Ser Glu Leu Ser Leu Val Asn Phe Glu Glu Ser 
            260                 265                 270         


Gln Thr Phe Phe Gly Leu Tyr Lys Leu 
        275                 280     


