               SEQUENCE LISTING

<110> Novartis AG

<120> ANTIBIOTIC-FREE METHOD FOR SELECTION OF TRANSFORMED BACTERIA
FIELD OF THE INVENTION


<130> PAT055452-EP-EPA

<160> 8

<170> BiSSAP 1.3

<210> 1
<211> 1197
<212> DNA
<213> Pseudomonas aeruginosa

<400> 1
atgatcatca aaccgcgcgt gcgtggcttc atctgcgtca ctacccatcc ggcaggctgc      60

gaggccaacg tcaagcaaca gatcgactac gtcgaggcca agggccccgt cgtcaacgga     120

ccgaagaagg tcctggtcat cggttcctcc accggctatg gcctggctgc ccgcatcacc     180

gccgcgttcg gttccggcgc cgataccctc ggggtgttct tcgagcgtcc gggcagcgag     240

agcaagccgg gcaccgccgg ctggtacaac tcggcggcct tcgagaagtt cgcccacgag     300

aaaggcctgt atgcccgcag catcaatggc gacgctttct ccgacgaggt gaagcggctg     360

accatcgaga ccatcaagcg cgatctcggc aaggtcgacc tggtggtcta cagcctggcc     420

gcgccgcgcc gtacccatcc gaagagcggc gaagtgttct cctcgaccct caagccgatc     480

ggcaagtcgg tcagcttccg tggcctggac accgacaagg aagtgatcaa ggacgtggtc     540

ctggaggcgg ccagcgacca ggaggtcgcc gataccgttg cggtgatggg cggcgaggac     600

tggcagatgt ggatcgacgc gctgctggag gccgacgtgc tggccgacgg cgcgaagacc     660

accgcgttca cctacctggg cgagaagatc acccacgaca tctactggaa cggttccatc     720

ggcgcagcca agaaggatct cgaccagaag gtcctcggta tccgcgacaa gctcgccccg     780

ctgggcggcg acgcgcgcgt ctcggtgctc aaggcggtgg tgacccaggc cagctcggcg     840

atcccgatga tgccgctgta cctgtcgctg ctgttcaagg tgatgaagga gcagggtacc     900

cacgaaggtt gcatcgagca ggtcgacggc ctgtaccggg agagcctgta cggcgccgag     960

ccgcgcctcg acgaggaagg ccgcctgcgt gccgactaca aggaactgca gccggaagtg    1020

cagtcccgtg tcgaggagct gtgggacaag gtaaccaacg agaaccttta cgaattgacc    1080

gacttcgccg gctacaagag cgagttcctc aacctgttcg gtttcgaggt cgctggggtc    1140

gactacgagc aggacgtcaa cccggacgtg cagatcgcca acctgatcca ggcctga       1197


<210> 2
<211> 398
<212> PRT
<213> Pseudomonas aeruginosa

<400> 2
Met Ile Ile Lys Pro Arg Val Arg Gly Phe Ile Cys Val Thr Thr His 
1               5                   10                  15      
Pro Ala Gly Cys Glu Ala Asn Val Lys Gln Gln Ile Asp Tyr Val Glu 
            20                  25                  30          
Ala Lys Gly Pro Val Val Asn Gly Pro Lys Lys Val Leu Val Ile Gly 
        35                  40                  45              
Ser Ser Thr Gly Tyr Gly Leu Ala Ala Arg Ile Thr Ala Ala Phe Gly 
    50                  55                  60                  
Ser Gly Ala Asp Thr Leu Gly Val Phe Phe Glu Arg Pro Gly Ser Glu 
65                  70                  75                  80  
Ser Lys Pro Gly Thr Ala Gly Trp Tyr Asn Ser Ala Ala Phe Glu Lys 
                85                  90                  95      
Phe Ala His Glu Lys Gly Leu Tyr Ala Arg Ser Ile Asn Gly Asp Ala 
            100                 105                 110         
Phe Ser Asp Glu Val Lys Arg Leu Thr Ile Glu Thr Ile Lys Arg Asp 
        115                 120                 125             
Leu Gly Lys Val Asp Leu Val Val Tyr Ser Leu Ala Ala Pro Arg Arg 
    130                 135                 140                 
Thr His Pro Lys Ser Gly Glu Val Phe Ser Ser Thr Leu Lys Pro Ile 
145                 150                 155                 160 
Gly Lys Ser Val Ser Phe Arg Gly Leu Asp Thr Asp Lys Glu Val Ile 
                165                 170                 175     
Lys Asp Val Val Leu Glu Ala Ala Ser Asp Gln Glu Val Ala Asp Thr 
            180                 185                 190         
Val Ala Val Met Gly Gly Glu Asp Trp Gln Met Trp Ile Asp Ala Leu 
        195                 200                 205             
Leu Glu Ala Asp Val Leu Ala Asp Gly Ala Lys Thr Thr Ala Phe Thr 
    210                 215                 220                 
Tyr Leu Gly Glu Lys Ile Thr His Asp Ile Tyr Trp Asn Gly Ser Ile 
225                 230                 235                 240 
Gly Ala Ala Lys Lys Asp Leu Asp Gln Lys Val Leu Gly Ile Arg Asp 
                245                 250                 255     
Lys Leu Ala Pro Leu Gly Gly Asp Ala Arg Val Ser Val Leu Lys Ala 
            260                 265                 270         
Val Val Thr Gln Ala Ser Ser Ala Ile Pro Met Met Pro Leu Tyr Leu 
        275                 280                 285             
Ser Leu Leu Phe Lys Val Met Lys Glu Gln Gly Thr His Glu Gly Cys 
    290                 295                 300                 
Ile Glu Gln Val Asp Gly Leu Tyr Arg Glu Ser Leu Tyr Gly Ala Glu 
305                 310                 315                 320 
Pro Arg Leu Asp Glu Glu Gly Arg Leu Arg Ala Asp Tyr Lys Glu Leu 
                325                 330                 335     
Gln Pro Glu Val Gln Ser Arg Val Glu Glu Leu Trp Asp Lys Val Thr 
            340                 345                 350         
Asn Glu Asn Leu Tyr Glu Leu Thr Asp Phe Ala Gly Tyr Lys Ser Glu 
        355                 360                 365             
Phe Leu Asn Leu Phe Gly Phe Glu Val Ala Gly Val Asp Tyr Glu Gln 
    370                 375                 380                 
Asp Val Asn Pro Asp Val Gln Ile Ala Asn Leu Ile Gln Ala 
385                 390                 395             

<210> 3
<211> 5450
<212> DNA
<213> Artificial Sequence

<220> 
<223> pFabV (empty vector with fabV gene and multiple cloning site)

<400> 3
atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa       60

ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt      120

tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt      180

cgacggagct cgaattcgga tcccgaccca tttgctgtcc accagtcatg ctagccatat      240

gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca      300

caattcccct atagtgagtc gtattaattt cgcgggatcg agatctcgat cctctacgcc      360

ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc      420

gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc      480

gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca      540

ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg      600

caggagtcgc ataagggaga gcgtcgagat cccggacacc atcgaatggc gcaaaacctt      660

tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc      720

agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt      780

ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc      840

ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct      900

gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat      960

taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg     1020

cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat     1080

cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt     1140

tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca     1200

tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc     1260

gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa     1320

atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat     1380

gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct     1440

ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg     1500

cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat     1560

cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg     1620

cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact     1680

ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc     1740

cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca     1800

acgcaattaa tgtaagttag ctcactcatt aggcaccggg atctcgaccg atgcccttga     1860

gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac     1920

ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca     1980

ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat     2040

tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg     2100

gcgagaagca ggccattatc gccggcatgg cggccccacg ggtgcgcatg atcgtgctcc     2160

tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac     2220

cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa     2280

catgaatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta     2340

tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat     2400

aaatgcttca ataatattga aaaaggaaga gtatgatcat caaaccgcgc gtgcgtggct     2460

tcatctgcgt cactacccat ccggcaggct gcgaggccaa cgtcaagcaa cagatcgact     2520

acgtcgaggc caagggcccc gtcgtcaacg gaccgaagaa ggtcctggtc atcggttcct     2580

ccaccggcta tggcctggct gcccgcatca ccgccgcgtt cggttccggc gccgataccc     2640

tcggggtgtt cttcgagcgt ccgggcagcg agagcaagcc gggcaccgcc ggctggtaca     2700

actcggcggc cttcgagaag ttcgcccacg agaaaggcct gtatgcccgc agcatcaatg     2760

gcgacgcttt ctccgacgag gtgaagcggc tgaccatcga gaccatcaag cgcgatctcg     2820

gcaaggtcga cctggtggtc tacagcctgg ccgcgccgcg ccgtacccat ccgaagagcg     2880

gcgaagtgtt ctcctcgacc ctcaagccga tcggcaagtc ggtcagcttc cgtggcctgg     2940

acaccgacaa ggaagtgatc aaggacgtgg tcctggaggc ggccagcgac caggaggtcg     3000

ccgataccgt tgcggtgatg ggcggcgagg actggcagat gtggatcgac gcgctgctgg     3060

aggccgacgt gctggccgac ggcgcgaaga ccaccgcgtt cacctacctg ggcgagaaga     3120

tcacccacga catctactgg aacggttcca tcggcgcagc caagaaggat ctcgaccaga     3180

aggtcctcgg tatccgcgac aagctcgccc cgctgggcgg cgacgcgcgc gtctcggtgc     3240

tcaaggcggt ggtgacccag gccagctcgg cgatcccgat gatgccgctg tacctgtcgc     3300

tgctgttcaa ggtgatgaag gagcagggta cccacgaagg ttgcatcgag caggtcgacg     3360

gcctgtaccg ggagagcctg tacggcgccg agccgcgcct cgacgaggaa ggccgcctgc     3420

gtgccgacta caaggaactg cagccggaag tgcagtcccg tgtcgaggag ctgtgggaca     3480

aggtaaccaa cgagaacctt tacgaattga ccgacttcgc cggctacaag agcgagttcc     3540

tcaacctgtt cggtttcgag gtcgctgggg tcgactacga gcaggacgtc aacccggacg     3600

tgcagatcgc caacctgatc caggcctgaa aactcaacga gctggacgcg gatgaacagg     3660

cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc     3720

gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt     3780

gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg     3840

ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa     3900

ctatgcggca tcagagcaga ttgtactgag agtgcaccat atatgcggtg tgaaataccg     3960

cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac     4020

tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata     4080

cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa     4140

aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct     4200

gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa     4260

agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg     4320

cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca     4380

cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa     4440

ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg     4500

gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg     4560

tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg     4620

acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc     4680

tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag     4740

attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac     4800

gctcagtgga acgaaaactc acgttaaggg attttggtca tgaacaataa aactgtctgc     4860

ttacataaac agtaatacaa ggggtgttta agaattaatt catgagcgga tacatatttg     4920

aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac     4980

ctgaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct     5040

cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg     5100

agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact     5160

ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac     5220

cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga     5280

gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga     5340

aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca     5400

ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca                5450


<210> 4
<211> 6263
<212> RNA
<213> Artificial Sequence

<220> 
<223> empty vector with fabV gene, multiple cloning site and kanamycin
      resistance gene

<400> 4
atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa       60

ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt      120

tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt      180

cgacggagct cgaattcgga tcccgaccca tttgctgtcc accagtcatg ctagccatat      240

gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca      300

caattcccct atagtgagtc gtattaattt cgcgggatcg agatctcgat cctctacgcc      360

ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc      420

gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc      480

gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca      540

ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg      600

caggagtcgc ataagggaga gcgtcgagat cccggacacc atcgaatggc gcaaaacctt      660

tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc      720

agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt      780

ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc      840

ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct      900

gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat      960

taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg     1020

cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat     1080

cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt     1140

tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca     1200

tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc     1260

gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa     1320

atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat     1380

gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct     1440

ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg     1500

cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat     1560

cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg     1620

cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact     1680

ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc     1740

cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca     1800

acgcaattaa tgtaagttag ctcactcatt aggcaccggg atctcgaccg atgcccttga     1860

gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac     1920

ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca     1980

ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat     2040

tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg     2100

gcgagaagca ggccattatc gccggcatgg cggccccacg ggtgcgcatg atcgtgctcc     2160

tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac     2220

cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa     2280

catgaatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta     2340

tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat     2400

aaatgcttca ataatattga aaaaggaaga gtatgatcat caaaccgcgc gtgcgtggct     2460

tcatctgcgt cactacccat ccggcaggct gcgaggccaa cgtcaagcaa cagatcgact     2520

acgtcgaggc caagggcccc gtcgtcaacg gaccgaagaa ggtcctggtc atcggttcct     2580

ccaccggcta tggcctggct gcccgcatca ccgccgcgtt cggttccggc gccgataccc     2640

tcggggtgtt cttcgagcgt ccgggcagcg agagcaagcc gggcaccgcc ggctggtaca     2700

actcggcggc cttcgagaag ttcgcccacg agaaaggcct gtatgcccgc agcatcaatg     2760

gcgacgcttt ctccgacgag gtgaagcggc tgaccatcga gaccatcaag cgcgatctcg     2820

gcaaggtcga cctggtggtc tacagcctgg ccgcgccgcg ccgtacccat ccgaagagcg     2880

gcgaagtgtt ctcctcgacc ctcaagccga tcggcaagtc ggtcagcttc cgtggcctgg     2940

acaccgacaa ggaagtgatc aaggacgtgg tcctggaggc ggccagcgac caggaggtcg     3000

ccgataccgt tgcggtgatg ggcggcgagg actggcagat gtggatcgac gcgctgctgg     3060

aggccgacgt gctggccgac ggcgcgaaga ccaccgcgtt cacctacctg ggcgagaaga     3120

tcacccacga catctactgg aacggttcca tcggcgcagc caagaaggat ctcgaccaga     3180

aggtcctcgg tatccgcgac aagctcgccc cgctgggcgg cgacgcgcgc gtctcggtgc     3240

tcaaggcggt ggtgacccag gccagctcgg cgatcccgat gatgccgctg tacctgtcgc     3300

tgctgttcaa ggtgatgaag gagcagggta cccacgaagg ttgcatcgag caggtcgacg     3360

gcctgtaccg ggagagcctg tacggcgccg agccgcgcct cgacgaggaa ggccgcctgc     3420

gtgccgacta caaggaactg cagccggaag tgcagtcccg tgtcgaggag ctgtgggaca     3480

aggtaaccaa cgagaacctt tacgaattga ccgacttcgc cggctacaag agcgagttcc     3540

tcaacctgtt cggtttcgag gtcgctgggg tcgactacga gcaggacgtc aacccggacg     3600

tgcagatcgc caacctgatc caggcctgaa aactcaacga gctggacgcg gatgaacagg     3660

cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc     3720

gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt     3780

gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg     3840

ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa     3900

ctatgcggca tcagagcaga ttgtactgag agtgcaccat atatgcggtg tgaaataccg     3960

cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac     4020

tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata     4080

cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa     4140

aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct     4200

gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa     4260

agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg     4320

cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca     4380

cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa     4440

ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg     4500

gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg     4560

tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg     4620

acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc     4680

tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag     4740

attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac     4800

gctcagtgga acgaaaactc acgttaaggg attttggtca tgaacaataa aactgtctgc     4860

ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcttgctc     4920

taggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga     4980

taatgtcggg caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga     5040

gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag     5100

actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc     5160

tgatgatgca tggttactca ccactgcgat ccccgggaaa acagcattcc aggtattaga     5220

agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt     5280

gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca     5340

ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa     5400

tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga     5460

ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt     5520

aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat     5580

cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata     5640

tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt     5700

ctaagaatta attcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag     5760

gggttccgcg cacatttccc cgaaaagtgc cacctgaaat tgtaaacgtt aatattttgt     5820

taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg     5880

gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt     5940

ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct     6000

atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt     6060

gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa     6120

agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc     6180

tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc     6240

tacagggcgc gtcccattcg cca                                             6263


<210> 5
<211> 720
<212> DNA
<213> Artificial Sequence

<220> 
<223> Green Fluorescent Protein (GFP)

<400> 5
atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac      60

ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac     120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc     180

ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag     240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc     300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg     360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac     420

aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac     480

ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc     540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac     600

tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc     660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa     720


<210> 6
<211> 6908
<212> DNA
<213> Artificial Sequence

<220> 
<223> pFabV-gfp (pFabV containing gfp gene and kanamycin resistance
      gene)

<400> 6
atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa       60

ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt      120

tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagttacttg tacagctcgt      180

ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct      240

tctcgttggg gtctttgctc agggcggact gggtgctcag gtagtggttg tcgggcagca      300

gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc      360

cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg      420

ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc      480

cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga      540

acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct      600

ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt      660

agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg      720

gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct      780

cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga      840

tgggcaccac cccggtgaac agctcctcgc ccttgctcac catatgtata tctccttctt      900

aaagttaaac aaaattattt ctagagggga attgttatcc gctcacaatt cccctatagt      960

gagtcgtatt aatttcgcgg gatcgagatc tcgatcctct acgccggacg catcgtggcc     1020

ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg     1080

gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca     1140

ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg     1200

gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag     1260

ggagagcgtc gagatcccgg acaccatcga atggcgcaaa acctttcgcg gtatggcatg     1320

atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgttatacga     1380

tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag     1440

ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat     1500

tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac     1560

ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga     1620

tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa     1680

agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct     1740

ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct     1800

tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg     1860

actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc     1920

attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa     1980

tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca     2040

aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca     2100

gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat     2160

ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac     2220

catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc     2280

tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac     2340

caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca     2400

gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtaa     2460

gttagctcac tcattaggca ccgggatctc gaccgatgcc cttgagagcc ttcaacccag     2520

tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct     2580

ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc     2640

gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg     2700

ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag aagcaggcca     2760

ttatcgccgg catggcggcc ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc     2820

ggctaggctg gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa     2880

cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtttctt     2940

agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct     3000

aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat     3060

attgaaaaag gaagagtatg atcatcaaac cgcgcgtgcg tggcttcatc tgcgtcacta     3120

cccatccggc aggctgcgag gccaacgtca agcaacagat cgactacgtc gaggccaagg     3180

gccccgtcgt caacggaccg aagaaggtcc tggtcatcgg ttcctccacc ggctatggcc     3240

tggctgcccg catcaccgcc gcgttcggtt ccggcgccga taccctcggg gtgttcttcg     3300

agcgtccggg cagcgagagc aagccgggca ccgccggctg gtacaactcg gcggccttcg     3360

agaagttcgc ccacgagaaa ggcctgtatg cccgcagcat caatggcgac gctttctccg     3420

acgaggtgaa gcggctgacc atcgagacca tcaagcgcga tctcggcaag gtcgacctgg     3480

tggtctacag cctggccgcg ccgcgccgta cccatccgaa gagcggcgaa gtgttctcct     3540

cgaccctcaa gccgatcggc aagtcggtca gcttccgtgg cctggacacc gacaaggaag     3600

tgatcaagga cgtggtcctg gaggcggcca gcgaccagga ggtcgccgat accgttgcgg     3660

tgatgggcgg cgaggactgg cagatgtgga tcgacgcgct gctggaggcc gacgtgctgg     3720

ccgacggcgc gaagaccacc gcgttcacct acctgggcga gaagatcacc cacgacatct     3780

actggaacgg ttccatcggc gcagccaaga aggatctcga ccagaaggtc ctcggtatcc     3840

gcgacaagct cgccccgctg ggcggcgacg cgcgcgtctc ggtgctcaag gcggtggtga     3900

cccaggccag ctcggcgatc ccgatgatgc cgctgtacct gtcgctgctg ttcaaggtga     3960

tgaaggagca gggtacccac gaaggttgca tcgagcaggt cgacggcctg taccgggaga     4020

gcctgtacgg cgccgagccg cgcctcgacg aggaaggccg cctgcgtgcc gactacaagg     4080

aactgcagcc ggaagtgcag tcccgtgtcg aggagctgtg ggacaaggta accaacgaga     4140

acctttacga attgaccgac ttcgccggct acaagagcga gttcctcaac ctgttcggtt     4200

tcgaggtcgc tggggtcgac tacgagcagg acgtcaaccc ggacgtgcag atcgccaacc     4260

tgatccaggc ctgaaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat     4320

cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg     4380

gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg     4440

ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag     4500

ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga     4560

gcagattgta ctgagagtgc accatatatg cggtgtgaaa taccgcacag atgcgtaagg     4620

agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc     4680

gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa     4740

tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt     4800

aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa     4860

aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt     4920

ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg     4980

tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc     5040

agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc     5100

gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta     5160

tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct     5220

acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc     5280

tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa     5340

caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa     5400

aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa     5460

aactcacgtt aagggatttt ggtcatgaac aataaaactg tctgcttaca taaacagtaa     5520

tacaaggggt gttatgagcc atattcaacg ggaaacgtct tgctctaggc cgcgattaaa     5580

ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc     5640

aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca     5700

tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac     5760

ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt     5820

actcaccact gcgatccccg ggaaaacagc attccaggta ttagaagaat atcctgattc     5880

aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt     5940

ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat     6000

gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga     6060

acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca     6120

tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga     6180

tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct     6240

cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc     6300

tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaag aattaattca     6360

tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat     6420

ttccccgaaa agtgccacct gaaattgtaa acgttaatat tttgttaaaa ttcgcgttaa     6480

atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata     6540

aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac     6600

tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc     6660

cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa     6720

atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg     6780

cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg     6840

tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc     6900

attcgcca                                                              6908


<210> 7
<211> 5309
<212> DNA
<213> Artificial Sequence

<220> 
<223> pET24

<400> 7
atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa       60

ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt      120

tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt      180

cgacggagct cgaattcgga tcccgaccca tttgctgtcc accagtcatg ctagccatat      240

gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca      300

caattcccct atagtgagtc gtattaattt cgcgggatcg agatctcgat cctctacgcc      360

ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc      420

gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc      480

gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca      540

ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg      600

caggagtcgc ataagggaga gcgtcgagat cccggacacc atcgaatggc gcaaaacctt      660

tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc      720

agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt      780

ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc      840

ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct      900

gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat      960

taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg     1020

cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat     1080

cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt     1140

tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca     1200

tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc     1260

gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa     1320

atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat     1380

gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct     1440

ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg     1500

cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat     1560

cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg     1620

cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact     1680

ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc     1740

cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca     1800

acgcaattaa tgtaagttag ctcactcatt aggcaccggg atctcgaccg atgcccttga     1860

gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac     1920

ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca     1980

ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat     2040

tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg     2100

gcgagaagca ggccattatc gccggcatgg cggccccacg ggtgcgcatg atcgtgctcc     2160

tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac     2220

cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa     2280

catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct     2340

gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt ggaacaccta     2400

catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg tcccgccgca     2460

tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt tcatcatcag     2520

taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc atgaacagaa     2580

atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt aacatggccc     2640

gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg     2700

aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac cgcagctgcc     2760

tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca     2820

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg     2880

ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg     2940

gcttaactat gcggcatcag agcagattgt actgagagtg caccatatat gcggtgtgaa     3000

ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc     3060

actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg     3120

gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc     3180

cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc     3240

ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga     3300

ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc     3360

ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat     3420

agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg     3480

cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc     3540

aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga     3600

gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact     3660

agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt     3720

ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag     3780

cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg     3840

tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgaa caataaaact     3900

gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc     3960

ttgctctagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc     4020

tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc     4080

gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat     4140

ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg     4200

tactcctgat gatgcatggt tactcaccac tgcgatcccc gggaaaacag cattccaggt     4260

attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg     4320

ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct     4380

cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga     4440

gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc     4500

accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg     4560

gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct     4620

tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca     4680

aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga     4740

gtttttctaa gaattaattc atgagcggat acatatttga atgtatttag aaaaataaac     4800

aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaaattgta aacgttaata     4860

ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg     4920

aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc     4980

cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa     5040

ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt     5100

cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac     5160

ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta     5220

gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg     5280

cgccgctaca gggcgcgtcc cattcgcca                                       5309


<210> 8
<211> 5918
<212> DNA
<213> Artificial Sequence

<220> 
<223> pET24-gfp

<400> 8
ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc       60

agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc      120

tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg      180

ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca      240

cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc      300

tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct      360

tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa      420

caaaaattta acgcgaattt taacaaaata ttaacgttta caatttcagg tggcactttt      480

cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat      540

ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt      600

catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa      660

ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg      720

tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa      780

atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca      840

gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc      900

gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca      960

attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt     1020

ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt     1080

ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat     1140

aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc     1200

tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt     1260

cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat     1320

gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc     1380

ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac     1440

gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag     1500

atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg     1560

tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca     1620

gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga     1680

actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca     1740

gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc     1800

agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca     1860

ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa     1920

aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc     1980

cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc     2040

gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg     2100

cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat     2160

cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca     2220

gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt     2280

attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac     2340

aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg     2400

gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg     2460

ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg     2520

ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg     2580

tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga     2640

agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg     2700

gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa     2760

cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt     2820

tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt     2880

caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct     2940

gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac     3000

gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag     3060

cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc     3120

cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggggccgcc     3180

atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag     3240

gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg     3300

ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg     3360

agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac     3420

cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa     3480

tgagtgagct aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac     3540

ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt     3600

gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac     3660

cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa     3720

atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta     3780

tcccactacc gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc     3840

gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag     3900

catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat     3960

cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga     4020

gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg     4080

ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg     4140

gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc     4200

atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt     4260

gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct     4320

ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag     4380

ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc     4440

cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt     4500

cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc     4560

atactctgcg acatcgtata acgttactgg tttcacattc accaccctga attgactctc     4620

ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat     4680

ctcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc     4740

cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc     4800

cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc     4860

gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg     4920

cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa     4980

attaatacga ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat     5040

tttgtttaac tttaagaagg agatatacat atggtgagca agggcgagga gctgttcacc     5100

ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg     5160

tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc     5220

accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag     5280

tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc     5340

gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc     5400

gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac     5460

ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac     5520

gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac     5580

aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc     5640

gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa     5700

gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc     5760

actctcggca tggacgagct gtacaagtaa tgctaacaaa gcccgaaagg aagctgagtt     5820

ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt     5880

gaggggtttt ttgctgaaag gaggaactat atccggat                             5918


