                         SEQUENCE LISTING

<110>  University of Manitoba
 
<120>  BIOREMEDIATION OF POLYCYCLIC AROMATIC HYDROCARBON AND/OR 
       POLYCHLORINATED BIPHENYL CONTAMINATION

<130>  AV87258WO

<140>  n/a
<141>  2015-05-01 

<150>  US 61/987,032
<151>  2014-05-01

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  1524
<212>  PRT
<213>  Staphylothermus marinus


<220>
<221>  misc_feature
<222>  (339)..(339)
<223>  Xaa can be any naturally occurring amino acid

<400>  1

Met Asn Arg Val Leu Ala Tyr Ser Leu Leu Ala Ile Met Thr Leu Ser 
1               5                   10                  15      


Leu Leu Ile Ile Pro Ala Pro Gly Ile Ala Gln Arg Ile Thr Val Gly 
            20                  25                  30          


Val Ser Val Lys Ala Gly Thr Tyr Asn Phe Tyr Asn Ile Thr Pro Thr 
        35                  40                  45              


Thr Gln Thr Val Glu Val Thr Asp Asn Gly Met Leu Arg Val Ile Ile 
    50                  55                  60                  


Asn Arg Thr Glu Ala Thr Glu Leu Gly Thr Thr Ile Arg Leu Ala Phe 
65                  70                  75                  80  


Ile Leu Asp Thr Asp Lys Tyr Asp Pro Asn Val Gly Gly Tyr Phe Leu 
                85                  90                  95      


Asn Val Ser Asn Ile Gly Val Tyr Ala Pro Ser Asp Pro Thr Gln Ser 
            100                 105                 110         


Pro Tyr Gly Gly Val Ile Asp Ile Thr Gln Asn Ser Thr Leu Thr Asp 
        115                 120                 125             


Gly Thr Gln Val Val Gly Asn Val Thr Val Val Asn Gly Gly Asn Asn 
    130                 135                 140                 


Val Ile Ile Leu Ile Asp Leu Ser Lys Leu Pro Asp Leu Gln Asn Val 
145                 150                 155                 160 


Val Tyr Ile Thr Asn Val Tyr Thr Glu Thr Ser Thr Thr Ala Asn Leu 
                165                 170                 175     


Thr Asn Thr Leu Leu Arg Val Lys Ala Phe Asp Ala Ala Ser Trp Asp 
            180                 185                 190         


Ala Val Ile Ser Gly Asn Gln Phe Lys Ile Leu Tyr Ile Pro Ser Leu 
        195                 200                 205             


Cys Lys Tyr Val Lys Ile Asn Val Ile His Ser Pro Ala Ile Val Gly 
    210                 215                 220                 


Thr Asn Val Asp Val Ile Val Ser Phe His Lys Tyr Phe Ser Leu Val 
225                 230                 235                 240 


Gln Ser Ile Ala Gly Val Ser Leu Asp Ile Thr Val Asp Asn Lys Thr 
                245                 250                 255     


Gln Leu Asn Met Thr Asn Tyr Tyr Asn Ala Thr Glu Asn Tyr Val Leu 
            260                 265                 270         


Ala Thr Phe Ile Asn Gly Asn Leu Thr Val Gly Gly Ser Glu Val Phe 
        275                 280                 285             


Ser Ser Pro Thr Val Thr Val Ile Asn Ala Ser Thr Phe Lys Tyr Ser 
    290                 295                 300                 


Gly Gln Val Lys Asp Tyr Ala Pro Thr Val Ala Asp Thr Ala Thr Pro 
305                 310                 315                 320 


Trp Val Arg Thr Leu Asn Lys Phe Glu Val Glu Phe Arg His Glu Ile 
                325                 330                 335     


Val Asn Xaa Thr His Asp Leu Ile Phe Tyr Ile His Cys Asp Ser Asp 
            340                 345                 350         


Thr Val Ser Tyr Asp Thr Trp Pro Phe Leu Ile Val Asn Ala Ser Leu 
        355                 360                 365             


Asp Ile Thr Thr Thr Glu Val Ala Phe Asn Ser Thr Thr Ile Asn Pro 
    370                 375                 380                 


Gly Asp Ile Val Asn Phe Thr Ala His Asn Val Pro Leu Gln Tyr Leu 
385                 390                 395                 400 


Thr Ala Thr Asn Tyr Gly Val Leu Arg Phe Gln Leu Ile Asn Pro Ala 
                405                 410                 415     


Leu Val Val Tyr Val Pro Val Ser Asn Met Thr Leu Ser Ala Asn Thr 
            420                 425                 430         


Thr Thr Gly Ile Ile Asn Gly Ser Phe Val Leu Pro Asp Ala Pro Tyr 
        435                 440                 445             


Gly Gly Leu Asp Tyr Leu Thr Tyr Leu Val Phe Asn Asp Gly Lys Phe 
    450                 455                 460                 


Ile Ala Asn Gly Tyr Ile Thr Val Ser Pro Cys Ile Glu Thr Tyr Val 
465                 470                 475                 480 


Leu Thr Asn Thr Ser Ala Tyr Ala Glu Asp Ala Gly Ser Ser Tyr Ile 
                485                 490                 495     


Gly Arg Phe Val Pro Gly Tyr Thr Ser Val Pro Gly Asp Tyr Ile Val 
            500                 505                 510         


Ile Lys Gly Tyr Gly Phe Ala Leu Ser Asn Leu Thr Gly Phe Thr Val 
        515                 520                 525             


Ser Ile Asn Asn Thr Asp Val Ile Ile Leu Asn Ala Thr Tyr Asn Ala 
    530                 535                 540                 


Ser Thr Gly Lys Ile Ile Ile Leu Ala Lys Leu Leu Asp Thr Asn Gly 
545                 550                 555                 560 


Thr Pro Ile Pro Val Gly Ala Gly Phe Ile Arg Val Gly Gln Asn Gly 
                565                 570                 575     


Thr Thr Asn Ile Ala Tyr Ala Pro Phe Asn Val Thr Arg Asn Ser Gly 
            580                 585                 590         


Leu Glu Lys Val Leu Phe Asn Pro Arg Trp Phe Tyr Asn Gly Thr Tyr 
        595                 600                 605             


Tyr Ile Glu His Asp Lys Leu Gly Asp Pro Tyr Leu Tyr Phe Pro Val 
    610                 615                 620                 


Asp Tyr Pro Leu Val Asn Asn Thr Phe Thr Thr Glu Met Trp Pro Phe 
625                 630                 635                 640 


Asn Thr Thr Ile Glu Val Ile Gly Trp Pro Thr Asn Thr Phe Thr Leu 
                645                 650                 655     


Lys Ala Phe Asn Lys Glu Phe Asn Leu Ser Phe Asn Leu Leu Thr Leu 
            660                 665                 670         


Ser Leu Thr Asn Gly Tyr Asn Met Thr Asn Leu Tyr Asn Leu Thr Ile 
        675                 680                 685             


Pro Phe Leu Pro Tyr Gly Asn Tyr Thr Leu Leu Glu Gly Thr Leu Leu 
    690                 695                 700                 


Ser Val Asn Asn Arg Thr Val Phe Thr Val His Met Gly Ile Asn Val 
705                 710                 715                 720 


Asp Leu Asp Ser Cys Gly Asn Gly Thr Leu Ser Ile Thr Val Val Gly 
                725                 730                 735     


Ala Ala Pro Asn Thr Glu Tyr Asn Phe Thr Phe Gly Tyr Gln Val His 
            740                 745                 750         


Asp Leu Asn Tyr Gly Ile Thr Arg Tyr Ile Ser Pro Gln Trp Asn Gly 
        755                 760                 765             


Thr Trp Asn Ile Ser Leu Val Thr Asp Ile Tyr Gly Thr Gly Ser Thr 
    770                 775                 780                 


Ser Val Pro Leu Ile Thr Leu Tyr Pro Thr Ser Tyr Val Ile Asn Ala 
785                 790                 795                 800 


Thr Trp Asp Val Ile Thr Trp Leu Arg Leu Ser Gly Ser Gly Thr Leu 
                805                 810                 815     


Asp Leu Leu Phe Ser Val Asp Val Ser Tyr Asn Gly Phe Thr Asp Asn 
            820                 825                 830         


Leu Thr Thr Pro Ile Thr Tyr Val Phe Gly Pro Ser Asp Thr Thr Pro 
        835                 840                 845             


Gly Ser Phe Asn Ile Tyr Val Asn Thr Thr Tyr Asn Val Ser Val Val 
    850                 855                 860                 


Arg Val Ala Val Asp Tyr Leu Pro Arg Thr Asn Val Val Ile Ser Val 
865                 870                 875                 880 


Pro Glu Thr Val Leu Pro Gly Asp Thr Ile Thr Val Gln Ile Phe Pro 
                885                 890                 895     


His His Asn Glu Val Trp Gly Phe Ile Glu Pro Thr Ala Leu Phe Asp 
            900                 905                 910         


Glu Asn Gln Leu Leu Gly Trp Tyr Leu Thr Val Arg Leu Val Asp Pro 
        915                 920                 925             


Leu Ser Asn Thr Val Val Glu Arg Val Ala Gly Tyr Tyr Ala Gly Asn 
    930                 935                 940                 


Leu Ile Val Glu Asp Val Asp Gly Asp Gly Asp Asn Glu Val Trp Phe 
945                 950                 955                 960 


Val Val Asn Leu Thr Ala Pro Leu Val Leu Gly Val Asp Lys Thr Tyr 
                965                 970                 975     


Arg Val Asp Val Glu Leu Phe Leu Ala Val Leu Asn Pro Ser Ser Asn 
            980                 985                 990         


Ile Thr Gly Val Thr Ala Val Asp  Asn Glu Cys Tyr Val  Gln Leu Asp 
        995                 1000                 1005             


Leu Asn  Gly Thr Ile Tyr Trp  Asn Gly Leu Gly Ser  Gly Ile Met 
    1010                 1015                 1020             


Leu Gly  Gly Asp Gly Gln Ile  Val Thr Val Leu Gly  Val Leu Glu 
    1025                 1030                 1035             


Gly Lys  Leu Asp Thr Ile Lys  Asp Gly Ile Ala Glu  Ile Asn Ala 
    1040                 1045                 1050             


Thr Val  Asn Asp Ile Asn Thr  Tyr Leu Lys Val Asn  Val Thr Asp 
    1055                 1060                 1065             


Leu Leu  Lys Thr Ile Asn Asn  Ser Val Val Met Ile  Lys Asn Asp 
    1070                 1075                 1080             


Thr Ala  Thr Leu Ile Ile Gly  Gln Ala Glu Ile Lys  Ala Lys Leu 
    1085                 1090                 1095             


Asp Asp  Leu Leu Asn Leu Thr  Ser Gln Val Asn Asp  Thr Val Thr 
    1100                 1105                 1110             


Met Ile  Leu Ala Cys Cys Asn  Asn Ala Ser Lys Val  Leu Asn Arg 
    1115                 1120                 1125             


Met Glu  Gly Thr Leu Asn Ser  Thr Tyr Thr Gly Val  Leu Asn Val 
    1130                 1135                 1140             


Lys Ser  Asp Leu Ser Thr Leu  Ile Asp Thr Ala Asn  Asn Val Val 
    1145                 1150                 1155             


Ile Pro  Lys Phe Asn Glu Leu  Tyr Asp Asn Val Thr  Val Glu Ile 
    1160                 1165                 1170             


Asn Ala  Ser Arg Asp Leu Ile  Ile Gln Lys Ile Ser  Ser Val Asn 
    1175                 1180                 1185             


Asp Ser  Leu Thr Thr Ile Ile  Ser Ala Gly Phe Asn  Asp Val Glu 
    1190                 1195                 1200             


Ala Met  Ile Ser Asn Leu Asn  Thr Thr Leu Leu Asn  Arg Ile Asp 
    1205                 1210                 1215             


Glu Leu  Glu Gly Thr Leu Leu  Phe Tyr Met Thr Ala  Asn Glu Gln 
    1220                 1225                 1230             


Arg Leu  Glu Gly Ile Ile Asn  Glu Thr Ala Asp Asp  Ile Val Tyr 
    1235                 1240                 1245             


Arg Leu  Thr Val Ile Ile Asp  Asp Arg Tyr Glu Ser  Leu Lys Asn 
    1250                 1255                 1260             


Leu Ile  Thr Leu Arg Ala Asp  Arg Leu Glu Met Ile  Ile Asn Asp 
    1265                 1270                 1275             


Asn Val  Ser Thr Ile Leu Ala  Ser Ile Gly Asn Val  Asn Leu Thr 
    1280                 1285                 1290             


Val Phe  Asn Lys Leu Asn Asp  Leu Glu Ile Glu Leu  Gly Asp Val 
    1295                 1300                 1305             


Asn Ala  Thr Ile Asn Ala Gly  Ile Phe Gln Ile Gln  Ser Asn Leu 
    1310                 1315                 1320             


Gly Asn  Ala Asn Gln Leu Ile  Leu Asp Thr Leu Thr  Ser Ser Lys 
    1325                 1330                 1335             


Val Glu  Ile Leu Asn Ala Ile  Ser Ser Asn Ala Ser  Ala Ile Ser 
    1340                 1345                 1350             


Ser Glu  Ile His Asn Ala Val  Asn Gln Leu Ser Thr  Leu Val Leu 
    1355                 1360                 1365             


Gln Val  Asn Asp Thr Leu Thr  Leu Lys Ile Thr Gly  Glu Ala Asp 
    1370                 1375                 1380             


Asn Ile  Leu Asn Phe Leu Ser  Ser Leu Glu Gly Ser  Met Asn Thr 
    1385                 1390                 1395             


Gly Phe  Asn Asn Val Thr Ser  Thr Leu Ser Ala Val  Glu Asn Asn 
    1400                 1405                 1410             


Ile Leu  Gly Lys Ile Thr Asp  Thr Ser Asn Leu Leu  Ser Ser Lys 
    1415                 1420                 1425             


Ile Asp  Asn Thr Leu Ser Thr  Leu Gln Asp Leu Ile  Thr Ser Thr 
    1430                 1435                 1440             


Ser Asn  Asp Leu Lys Asn Ser  Ile Ser Ser Ala Lys  Asn Asp Ile 
    1445                 1450                 1455             


Val Ser  Ser Leu Ser Ser Lys  Val Asp Ser Ser Thr  Gln Thr Leu 
    1460                 1465                 1470             


Ser Thr  Lys Leu Asp Asp Leu  Lys Ser Ala Gln Glu  Ser Asn Thr 
    1475                 1480                 1485             


Asn Ser  Ile Asn Asn Asn Ile  Met Leu Phe Gly Ala  Ala Ser Leu 
    1490                 1495                 1500             


Ile Leu  Leu Ile Val Thr Ile  Gly Leu Val Gly Tyr  Arg Leu Ile 
    1505                 1510                 1515             


Ala Arg  Arg Arg Val Gly 
    1520                 


<210>  2
<211>  52
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RHCC polypeptide chain fragment of tetrabrachion

<400>  2

Gly Ser Ile Ile Asn Glu Thr Ala Asp Asp Ile Val Tyr Arg Leu Thr 
1               5                   10                  15      


Val Ile Ile Asp Asp Arg Tyr Glu Ser Leu Lys Asn Leu Ile Thr Leu 
            20                  25                  30          


Arg Ala Asp Arg Leu Glu Met Ile Ile Asn Asp Asn Val Ser Thr Ile 
        35                  40                  45              


Leu Ala Ser Ile 
    50          


<210>  3
<211>  156
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence encoding the RHCC polypeptide chain fragment 
       of the tetrabrachion protein

<400>  3
cgatccatca tcaacgaaac cgctgacgac atcgtttacc gtcgtaccgt tatcatcgac       60

gaccgttacg aatctctgaa aaacctgatc accctgctgg ctgaccgtct ggaaatgatc      120

atcaacgaca ccgtttctac catcctggct tctatc                                156


