                         SEQUENCE LISTING

<110>  The Trustees of the University of Pennsylvania
 
<120>  METHODS AND COMPOSITIONS FOR TREATING METASTATIC BREAST CANCER 
       AND OTHER BREAST CANCERS

<130>  14-7028PCT

<150>  61/984,686
<151>  2014-04-25

<160>  25    

<170>  PatentIn version 3.5

<210>  1
<211>  2717
<212>  DNA
<213>  Artificial sequence

<220>
<223>  engineered anti-HER antibody


<220>
<221>  misc
<222>  (1)..(60)
<223>  IL2 signal/leader peptide

<220>
<221>  CDS
<222>  (61)..(423)
<223>  heavy variable

<220>
<221>  Misc
<222>  (439)..(714)
<223>  CH1

<220>
<221>  Misc
<222>  (715)..(1410)
<223>  CH1

<220>
<221>  IRES
<222>  (1422)..(2009)

<220>
<221>  Misc
<222>  (2010)..(2069)
<223>  IL2 signal/leader peptide

<220>
<221>  Misc
<222>  (2070)..(2391)
<223>  variable light

<220>
<221>  Misc
<222>  (2407)..(2711)
<223>  light chain constant region

<400>  1
atgtaccgga tgcagctgct gagctgtatc gccctgtctc tggccctcgt gaccaacagc       60

gaa gtg cag ctg gtg gaa agc ggc gga gga ctg gtg cag cct ggc gga        108
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly           
1               5                   10                  15                

tct ctg aga ctg agc tgt gcc gcc agc ggc ttc aac atc aag gac acc        156
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys Asp Thr           
            20                  25                  30                    

tac atc cac tgg gtg cgc cag gcc cct ggc aag gga ctg gaa tgg gtg        204
Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val           
        35                  40                  45                        

gcc aga atc tac ccc acc aac ggc tac acc aga tac gcc gac agc gtg        252
Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser Val           
    50                  55                  60                            

aag ggc cgg ttc acc atc agc gcc gac acc agc aag aac acc gcc tac        300
Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr           
65                  70                  75                  80            

ctg cag atg aac agc ctg cgg gcc gag gac acc gcc gtg tac tac tgt        348
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys           
                85                  90                  95                

agt aga tgg gga ggc gac ggc ttc tac gcc atg gac tat tgg ggc cag        396
Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr Trp Gly Gln           
            100                 105                 110                   

ggc acc ctc gtg aca gtg tct agt gcg tcgaccaagg ggccctcggt              443
Gly Thr Leu Val Thr Val Ser Ser Ala                                       
        115                 120                                           

cttccccctg gcaccctcct ccaagagcac ctctgggggc acagcggccc tgggctgcct      503

ggtcaaggac tacttccccg aaccggtgac ggtgtcgtgg aactcaggcg ccctgaccag      563

cggcgtgcac accttcccgg ctgtcctaca gtcctcagga ctctactccc tcagcagcgt      623

ggtgaccgtg ccctccagca gcttgggcac ccagacctac atctgcaacg tgaatcacaa      683

gcccagcaac accaaggtgg acaagaaagt tgagcccaaa tcttgtgaca aaactcacac      743

atgcccaccg tgcccagcac ctgaactcct ggggggaccg tcagtcttcc tcttcccccc      803

aaaacccaag gacaccctca tgatctcccg gacccctgag gtcacatgcg tggtggtgga      863

cgtgagccac gaagaccctg aggtcaagtt caactggtac gtggacggcg tggaggtgca      923

taatgccaag acaaagccgc gggaggagca gtacaacagc acgtaccgtg tggtcagcgt      983

cctcaccgtc ctgcaccagg actggctgaa tggcaaggag tacaagtgca aggtctccaa     1043

caaagccctc ccagccccca tcgagaaaac catctccaaa gccaaagggc agccccgaga     1103

accacaggtg tacaccctgc ccccatcccg ggatgagctg accaagaacc aggtcagcct     1163

gacctgcctg gtcaaaggct tctatcccag cgacatcgcc gtggagtggg agagcaatgg     1223

gcagccggag aacaactaca agaccacgcc tcccgtgctg gactccgacg gctccttctt     1283

cctctacagc aagctcaccg tggacaagag caggtggcag caggggaacg tcttctcatg     1343

ctccgtgatg catgaggctc tgcacaacca ctacacgcag aagagcctct ccctgtctcc     1403

cggcaagtga taaggccggc ccctctccct cccccccccc taacgttact ggccgaagcc     1463

gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt     1523

ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc     1583

tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc     1643

tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc     1703

cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg     1763

cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct     1823

cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat     1883

ctgatctggg gcctcggtac acatgcttta catgtgttta gtcgaggtta aaaaaacgtc     1943

taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc     2003

acaaccatgt accgcatgca actcctgtct tgcattgcac taagtcttgc acttgtcaca     2063

aacagtgata tccagatgac ccagagcccc agcagcctgt ctgccagcgt gggcgacaga     2123

gtgaccatca cctgtagagc cagccaggac gtgaacaccg ccgtggcctg gtatcagcag     2183

aagcctggca aggcccccaa gctgctgatc tacagcgcca gcttcctgta cagcggcgtg     2243

cccagcagat tcagcggcag cagatccggc accgacttca ccctgaccat cagctccctg     2303

cagcccgagg acttcgccac ctactactgc cagcagcact acaccacccc ccccacattt     2363

ggccagggca ccaaggtgga aatcaagcgt acggtggctg caccatctgt cttcatcttc     2423

ccgccatctg atgagcagtt gaaatctgga actgcctctg ttgtgtgcct gctgaataac     2483

ttctacccca gagaagccaa agtgcagtgg aaggtggaca acgccctgca gagcggaaac     2543

agccaggaaa gcgtgacaga gcaggattcc aaggattcca catacagcct gagcagcaca     2603

ctgacactgt ccaaggccga ctacgagaag cacaaggtgt acgcctgcga agtgacacac     2663

cagggactgt cctcccctgt gacaaagagc ttcaacagag gagaatgctg ataa           2717


<210>  2
<211>  121
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  2

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys Asp Thr 
            20                  25                  30          


Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser Ala 
        115                 120     


<210>  3
<211>  470
<212>  PRT
<213>  Artificial sequence

<220>
<223>  engineered anti-HER heavy chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(20)
<223>  heavy chain signal peptide

<220>
<221>  MISC_FEATURE
<222>  (21)..(140)
<223>  heavy chain variable region

<220>
<221>  MISC_FEATURE
<222>  (141)..(259)
<223>  heavy chain constant region 1

<220>
<221>  MISC_FEATURE
<222>  (260)..(470)
<223>  heavy chain FC

<400>  3

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val 
            20                  25                  30          


Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn 
        35                  40                  45              


Ile Lys Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly 
    50                  55                  60                  


Leu Glu Trp Val Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr 
65                  70                  75                  80  


Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys 
                85                  90                  95      


Asn Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp 
        115                 120                 125             


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys 
    130                 135                 140                 


Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly 
145                 150                 155                 160 


Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
                165                 170                 175     


Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
            180                 185                 190         


Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
        195                 200                 205             


Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn 
    210                 215                 220                 


Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro 
225                 230                 235                 240 


Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 
                245                 250                 255     


Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
            260                 265                 270         


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
        275                 280                 285             


Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly 
    290                 295                 300                 


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn 
305                 310                 315                 320 


Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
                325                 330                 335     


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro 
            340                 345                 350         


Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
        355                 360                 365             


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn 
    370                 375                 380                 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
385                 390                 395                 400 


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
                405                 410                 415     


Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
            420                 425                 430         


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
        435                 440                 445             


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
    450                 455                 460                 


Ser Leu Ser Pro Gly Lys 
465                 470 


<210>  4
<211>  214
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Engineered anti-Her2 light chain


<220>
<221>  MISC_FEATURE
<222>  (1)..(20)
<223>  signal sequence

<220>
<221>  MISC_FEATURE
<222>  (21)..(130)
<223>  light chain variable region

<220>
<221>  MISC_FEATURE
<222>  (131)..(214)
<223>  light chain constant region

<400>  4

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser 
            20                  25                  30          


Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp 
        35                  40                  45              


Val Asn Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro 
    50                  55                  60                  


Lys Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser 
65                  70                  75                  80  


Arg Phe Ser Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
                85                  90                  95      


Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr 
            100                 105                 110         


Thr Thr Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg 
        115                 120                 125             


Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln 
    130                 135                 140                 


Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr 
145                 150                 155                 160 


Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser 
                165                 170                 175     


Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr 
            180                 185                 190         


Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys 
        195                 200                 205             


His Lys Val Tyr Ala Cys 
    210                 


<210>  5
<211>  6694
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pAAV.CB7.CI.201IA.rBG


<220>
<221>  polyA_signal
<222>  (60)..(186)

<220>
<221>  misc_feature
<222>  (275)..(404)
<223>  3' ITR (complement)

<220>
<221>  rep_origin
<222>  (581)..(1036)

<220>
<221>  misc_feature
<222>  (3226)..(3355)
<223>  5' ITR

<220>
<221>  misc
<222>  (3423)..(3804)
<223>  CMV IE promoter

<220>
<221>  promoter
<222>  (3807)..(4088)

<220>
<221>  TATA_signal
<222>  (4061)..(4064)

<220>
<221>  Intron
<222>  (4183)..(5155)

<220>
<221>  CDS
<222>  (5161)..(6690)
<223>  201IA

<400>  5
gtacctctag agtcgacccg ggcggcctcg aggacggggt gaactacgcc tgaggatccg       60

atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt      120

ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct      180

cactcggaag caattcgttg atctgaattt cgaccaccca taatacccat taccctggta      240

gataagtagc atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc      300

actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc      360

ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagccttaa ttaacctaat      420

tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat      480

cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat      540

cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca      600

ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta      660

gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt      720

caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac      780

cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt      840

tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga      900

acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg      960

gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata     1020

ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt     1080

tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc     1140

ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc     1200

ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa     1260

aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg     1320

gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag     1380

ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc     1440

gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta     1500

cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg     1560

cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca     1620

acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac     1680

caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat     1740

taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg     1800

ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata     1860

aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta     1920

agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa     1980

atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag     2040

tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg     2100

tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact     2160

gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg     2220

taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc     2280

aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata     2340

ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta     2400

catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc     2460

ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg     2520

ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac     2580

agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg     2640

taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt     2700

atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct     2760

cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg     2820

ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata     2880

accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca     2940

gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc     3000

gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg     3060

agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta     3120

tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca     3180

gctatgacca tgattacgcc agatttaatt aaggccttaa ttaggctgcg cgctcgctcg     3240

ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg cccggcctca     3300

gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg ttccttgtag     3360

ttaatgatta acccgccatg ctacttatct accagggtaa tggggatcct ctagaactat     3420

agctagtcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt     3480

tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg     3540

accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc     3600

aatagggact ttccattgac gtcaatgggt ggactattta cggtaaactg cccacttggc     3660

agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg     3720

gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat     3780

ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct     3840

ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg     3900

tgcagcgatg ggggcggggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga     3960

ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg     4020

aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg     4080

gcgggcgggg agtcgctgcg acgctgcctt cgccccgtgc cccgctccgc cgccgcctcg     4140

cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc     4200

ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg     4260

ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg     4320

ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc     4380

tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc     4440

gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg     4500

cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc     4560

cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt     4620

acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg     4680

ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg     4740

agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc     4800

gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc     4860

cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg     4920

ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg     4980

ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc     5040

tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc     5100

ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc     5160

atg gag ttc ggg ctg agc tgg gtc ttt ctg gtg gcc ctg ctg aag gga       5208
Met Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Leu Leu Lys Gly           
1               5                   10                  15                

gtc cag tgc gag gtg cag ctg ctg gaa tcc gga cct ggc ctg gtg aaa       5256
Val Gln Cys Glu Val Gln Leu Leu Glu Ser Gly Pro Gly Leu Val Lys           
            20                  25                  30                    

cca tct gag aca ctg agt ctg act tgt gct gtc tcc ggc ctg tct atc       5304
Pro Ser Glu Thr Leu Ser Leu Thr Cys Ala Val Ser Gly Leu Ser Ile           
        35                  40                  45                        

agc tcc gat ttc tcc tgg gca tgg att agg cag acc ccc ggc aag gcc       5352
Ser Ser Asp Phe Ser Trp Ala Trp Ile Arg Gln Thr Pro Gly Lys Ala           
    50                  55                  60                            

ctg gaa tat gtg ggg tac atc cgc ggg aac acc gga gat aca tac tat       5400
Leu Glu Tyr Val Gly Tyr Ile Arg Gly Asn Thr Gly Asp Thr Tyr Tyr           
65                  70                  75                  80            

aat cct agt ctg aag tca agg ctg act atc tca aag gac acc agc aaa       5448
Asn Pro Ser Leu Lys Ser Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys           
                85                  90                  95                

aac caa atc tac ctg aat ctg tct agt gtc acc gct ggc gat gcc gcc       5496
Asn Gln Ile Tyr Leu Asn Leu Ser Ser Val Thr Ala Gly Asp Ala Ala           
            100                 105                 110                   

gtg tac tat tgc gca agg gac cgg gtg tgc gac gat gac tac gga tac       5544
Val Tyr Tyr Cys Ala Arg Asp Arg Val Cys Asp Asp Asp Tyr Gly Tyr           
        115                 120                 125                       

tat tac acc gag gtg tgc ttc ggc ctg gat tct tgg ggg cag gga atc       5592
Tyr Tyr Thr Glu Val Cys Phe Gly Leu Asp Ser Trp Gly Gln Gly Ile           
    130                 135                 140                           

gtg gtc aca gtg tca agc ggc gga gga ggc agc gga gga gga ggg tcc       5640
Val Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser           
145                 150                 155                 160           

gga ggc ggg gga tct gca gaa ctg gtc atg aca cag tcc cca ctg agc       5688
Gly Gly Gly Gly Ser Ala Glu Leu Val Met Thr Gln Ser Pro Leu Ser           
                165                 170                 175               

ctg tcc gtc gct cca gga cag act gca tct att agt tgt cga tcc tct       5736
Leu Ser Val Ala Pro Gly Gln Thr Ala Ser Ile Ser Cys Arg Ser Ser           
            180                 185                 190                   

cag tcc ctg gac tat gct aac ggc aat acc tac ctg tct tgg ttt cac       5784
Gln Ser Leu Asp Tyr Ala Asn Gly Asn Thr Tyr Leu Ser Trp Phe His           
        195                 200                 205                       

cag cga cca gga cag cca cct cgg aga ctg atc tat cag att tcc aac       5832
Gln Arg Pro Gly Gln Pro Pro Arg Arg Leu Ile Tyr Gln Ile Ser Asn           
    210                 215                 220                           

aga gat tct gga gtg ccc gac agg ttc tca ggc agc gga gca gga act       5880
Arg Asp Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ala Gly Thr           
225                 230                 235                 240           

gag ttt acc ctg cga atc agt cgg atg gaa tca gat gac gtg ggg atc       5928
Glu Phe Thr Leu Arg Ile Ser Arg Met Glu Ser Asp Asp Val Gly Ile           
                245                 250                 255               

tac tac tgc gga cag ggg acc aca ttc cca cgg aca ttt gga cag ggc       5976
Tyr Tyr Cys Gly Gln Gly Thr Thr Phe Pro Arg Thr Phe Gly Gln Gly           
            260                 265                 270                   

act aag gtg gag atc aaa acc tgt gga gga gga agc aag cca cca acc       6024
Thr Lys Val Glu Ile Lys Thr Cys Gly Gly Gly Ser Lys Pro Pro Thr           
        275                 280                 285                       

tgc cct cca tgt aca tct ccc gaa ctg ctg ggc ggg cct agc gtg ttc       6072
Cys Pro Pro Cys Thr Ser Pro Glu Leu Leu Gly Gly Pro Ser Val Phe           
    290                 295                 300                           

ctg ttt ccc cct aag cct aaa gat aca ctg atg att agt aga acc cca       6120
Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro           
305                 310                 315                 320           

gag gtc aca tgc gtg gtc gtg gac gtg tcc cag gaa gat cct gac gtg       6168
Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Asp Val           
                325                 330                 335               

aag ttc aac tgg tac gtg aat ggc gcc gag gtg cac cat gct cag act       6216
Lys Phe Asn Trp Tyr Val Asn Gly Ala Glu Val His His Ala Gln Thr           
            340                 345                 350                   

aaa cca cgc gaa acc cag tat aat agt aca tac cga gtc gtg tca gtc       6264
Lys Pro Arg Glu Thr Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val           
        355                 360                 365                       

ctg aca gtg act cac cag gat tgg ctg aac ggc aag gag tat acc tgc       6312
Leu Thr Val Thr His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Thr Cys           
    370                 375                 380                           

aag gtg tct aac aag gcc ctg ccc gcc cct atc cag aaa aca att agc       6360
Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Gln Lys Thr Ile Ser           
385                 390                 395                 400           

aag gac aaa ggg cag cca cgg gaa ccc cag gtg tac act ctg cca ccc       6408
Lys Asp Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro           
                405                 410                 415               

tca aga gag gaa ctg act aag aac cag gtc agc ctg acc tgt ctg gtg       6456
Ser Arg Glu Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val           
            420                 425                 430                   

aaa ggc ttc tac ccc agc gat atc gtc gtg gag tgg gaa agt tca ggc       6504
Lys Gly Phe Tyr Pro Ser Asp Ile Val Val Glu Trp Glu Ser Ser Gly           
        435                 440                 445                       

cag cct gag aat act tac aag act acc cct cca gtg ctg gat agc gac       6552
Gln Pro Glu Asn Thr Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp           
    450                 455                 460                           

ggg tcc tat ttc ctg tac agc aag ctg aca gtg gac aaa tcc cgc tgg       6600
Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp           
465                 470                 475                 480           

cag cag gga aac gtc ttt tcc tgt tct gtg atg cat gag gcc ctg cac       6648
Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His           
                485                 490                 495               

aat cat tac acc cag aag agt ctg tca ctg agc ccc ggc aaa tgag          6694
Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys                   
            500                 505                 510                   


<210>  6
<211>  510
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  6

Met Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Leu Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Glu Val Gln Leu Leu Glu Ser Gly Pro Gly Leu Val Lys 
            20                  25                  30          


Pro Ser Glu Thr Leu Ser Leu Thr Cys Ala Val Ser Gly Leu Ser Ile 
        35                  40                  45              


Ser Ser Asp Phe Ser Trp Ala Trp Ile Arg Gln Thr Pro Gly Lys Ala 
    50                  55                  60                  


Leu Glu Tyr Val Gly Tyr Ile Arg Gly Asn Thr Gly Asp Thr Tyr Tyr 
65                  70                  75                  80  


Asn Pro Ser Leu Lys Ser Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys 
                85                  90                  95      


Asn Gln Ile Tyr Leu Asn Leu Ser Ser Val Thr Ala Gly Asp Ala Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg Asp Arg Val Cys Asp Asp Asp Tyr Gly Tyr 
        115                 120                 125             


Tyr Tyr Thr Glu Val Cys Phe Gly Leu Asp Ser Trp Gly Gln Gly Ile 
    130                 135                 140                 


Val Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Gly Gly Gly Gly Ser Ala Glu Leu Val Met Thr Gln Ser Pro Leu Ser 
                165                 170                 175     


Leu Ser Val Ala Pro Gly Gln Thr Ala Ser Ile Ser Cys Arg Ser Ser 
            180                 185                 190         


Gln Ser Leu Asp Tyr Ala Asn Gly Asn Thr Tyr Leu Ser Trp Phe His 
        195                 200                 205             


Gln Arg Pro Gly Gln Pro Pro Arg Arg Leu Ile Tyr Gln Ile Ser Asn 
    210                 215                 220                 


Arg Asp Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ala Gly Thr 
225                 230                 235                 240 


Glu Phe Thr Leu Arg Ile Ser Arg Met Glu Ser Asp Asp Val Gly Ile 
                245                 250                 255     


Tyr Tyr Cys Gly Gln Gly Thr Thr Phe Pro Arg Thr Phe Gly Gln Gly 
            260                 265                 270         


Thr Lys Val Glu Ile Lys Thr Cys Gly Gly Gly Ser Lys Pro Pro Thr 
        275                 280                 285             


Cys Pro Pro Cys Thr Ser Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 
    290                 295                 300                 


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
305                 310                 315                 320 


Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Asp Val 
                325                 330                 335     


Lys Phe Asn Trp Tyr Val Asn Gly Ala Glu Val His His Ala Gln Thr 
            340                 345                 350         


Lys Pro Arg Glu Thr Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
        355                 360                 365             


Leu Thr Val Thr His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Thr Cys 
    370                 375                 380                 


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Gln Lys Thr Ile Ser 
385                 390                 395                 400 


Lys Asp Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
                405                 410                 415     


Ser Arg Glu Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
            420                 425                 430         


Lys Gly Phe Tyr Pro Ser Asp Ile Val Val Glu Trp Glu Ser Ser Gly 
        435                 440                 445             


Gln Pro Glu Asn Thr Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
    450                 455                 460                 


Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
465                 470                 475                 480 


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
                485                 490                 495     


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
            500                 505                 510 


<210>  7
<211>  6694
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Plasmid encoding 201IA(I253A)mutant


<220>
<221>  misc
<222>  (1)..(130)
<223>  5' ITR

<220>
<221>  misc_feature
<222>  (198)..(579)
<223>  CMV IE promoter

<220>
<221>  promoter
<222>  (582)..(863)
<223>  CB promoter

<220>
<221>  TATA_signal
<222>  (836)..(839)

<220>
<221>  Intron
<222>  (958)..(1930)
<223>  chicken beta-actin intron

<220>
<221>  CDS
<222>  (1936)..(3465)
<223>  CMV IE promoter

<220>
<221>  polyA_signal
<222>  (3529)..(3655)
<223>  rabbit globin polyA

<220>
<221>  misc_feature
<222>  (3744)..(3873)
<223>  3' ITR (complement)

<220>
<221>  rep_origin
<222>  (4050)..(4505)

<220>
<221>  misc_feature
<222>  (4636)..(5493)
<223>  AP(R) marker

<220>
<221>  rep_origin
<222>  (5667)..(6255)

<400>  7
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact      120

aggggttcct tgtagttaat gattaacccg ccatgctact tatctaccag ggtaatgggg      180

atcctctaga actatagcta gtcgacattg attattgact agttattaat agtaatcaat      240

tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa      300

tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt      360

tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggact atttacggta      420

aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt      480

caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc      540

tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac      600

gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat      660

tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg      720

ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca      780

gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa      840

aaagcgaagc gcgcggcggg cggggagtcg ctgcgacgct gccttcgccc cgtgccccgc      900

tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg      960

agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt     1020

gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg     1080

gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc     1140

gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt     1200

gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg     1260

aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg     1320

gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg     1380

ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg     1440

caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg     1500

cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt     1560

ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg     1620

aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc     1680

ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc     1740

cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg     1800

gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat     1860

gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat     1920

tttggcaaag aattc atg gag ttc ggg ctg agc tgg gtc ttt ctg gtg gcc      1971
                 Met Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala          
                 1               5                   10                   

ctg ctg aag gga gtc cag tgc gag gtg cag ctg ctg gaa tcc gga cct       2019
Leu Leu Lys Gly Val Gln Cys Glu Val Gln Leu Leu Glu Ser Gly Pro           
        15                  20                  25                        

ggc ctg gtg aaa cca tct gag aca ctg agt ctg act tgt gct gtc tcc       2067
Gly Leu Val Lys Pro Ser Glu Thr Leu Ser Leu Thr Cys Ala Val Ser           
    30                  35                  40                            

ggc ctg tct atc agc tcc gat ttc tcc tgg gca tgg att agg cag acc       2115
Gly Leu Ser Ile Ser Ser Asp Phe Ser Trp Ala Trp Ile Arg Gln Thr           
45                  50                  55                  60            

ccc ggc aag gcc ctg gaa tat gtg ggg tac atc cgc ggg aac acc gga       2163
Pro Gly Lys Ala Leu Glu Tyr Val Gly Tyr Ile Arg Gly Asn Thr Gly           
                65                  70                  75                

gat aca tac tat aat cct agt ctg aag tca agg ctg act atc tca aag       2211
Asp Thr Tyr Tyr Asn Pro Ser Leu Lys Ser Arg Leu Thr Ile Ser Lys           
            80                  85                  90                    

gac acc agc aaa aac caa atc tac ctg aat ctg tct agt gtc acc gct       2259
Asp Thr Ser Lys Asn Gln Ile Tyr Leu Asn Leu Ser Ser Val Thr Ala           
        95                  100                 105                       

ggc gat gcc gcc gtg tac tat tgc gca agg gac cgg gtg tgc gac gat       2307
Gly Asp Ala Ala Val Tyr Tyr Cys Ala Arg Asp Arg Val Cys Asp Asp           
    110                 115                 120                           

gac tac gga tac tat tac acc gag gtg tgc ttc ggc ctg gat tct tgg       2355
Asp Tyr Gly Tyr Tyr Tyr Thr Glu Val Cys Phe Gly Leu Asp Ser Trp           
125                 130                 135                 140           

ggg cag gga atc gtg gtc aca gtg tca agc ggc gga gga ggc agc gga       2403
Gly Gln Gly Ile Val Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly           
                145                 150                 155               

gga gga ggg tcc gga ggc ggg gga tct gca gaa ctg gtc atg aca cag       2451
Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Glu Leu Val Met Thr Gln           
            160                 165                 170                   

tcc cca ctg agc ctg tcc gtc gct cca gga cag act gca tct att agt       2499
Ser Pro Leu Ser Leu Ser Val Ala Pro Gly Gln Thr Ala Ser Ile Ser           
        175                 180                 185                       

tgt cga tcc tct cag tcc ctg gac tat gct aac ggc aat acc tac ctg       2547
Cys Arg Ser Ser Gln Ser Leu Asp Tyr Ala Asn Gly Asn Thr Tyr Leu           
    190                 195                 200                           

tct tgg ttt cac cag cga cca gga cag cca cct cgg aga ctg atc tat       2595
Ser Trp Phe His Gln Arg Pro Gly Gln Pro Pro Arg Arg Leu Ile Tyr           
205                 210                 215                 220           

cag att tcc aac aga gat tct gga gtg ccc gac agg ttc tca ggc agc       2643
Gln Ile Ser Asn Arg Asp Ser Gly Val Pro Asp Arg Phe Ser Gly Ser           
                225                 230                 235               

gga gca gga act gag ttt acc ctg cga atc agt cgg atg gaa tca gat       2691
Gly Ala Gly Thr Glu Phe Thr Leu Arg Ile Ser Arg Met Glu Ser Asp           
            240                 245                 250                   

gac gtg ggg atc tac tac tgc gga cag ggg acc aca ttc cca cgg aca       2739
Asp Val Gly Ile Tyr Tyr Cys Gly Gln Gly Thr Thr Phe Pro Arg Thr           
        255                 260                 265                       

ttt gga cag ggc act aag gtg gag atc aaa acc tgt gga gga gga agc       2787
Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Thr Cys Gly Gly Gly Ser           
    270                 275                 280                           

aag cca cca acc tgc cct cca tgt aca tct ccc gaa ctg ctg ggc ggg       2835
Lys Pro Pro Thr Cys Pro Pro Cys Thr Ser Pro Glu Leu Leu Gly Gly           
285                 290                 295                 300           

cct agc gtg ttc ctg ttt ccc cct aag cct aaa gat aca ctg atg gcc       2883
Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ala           
                305                 310                 315               

agt aga acc cca gag gtc aca tgc gtg gtc gtg gac gtg tcc cag gaa       2931
Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu           
            320                 325                 330                   

gat cct gac gtg aag ttc aac tgg tac gtg aat ggc gcc gag gtg cac       2979
Asp Pro Asp Val Lys Phe Asn Trp Tyr Val Asn Gly Ala Glu Val His           
        335                 340                 345                       

cat gct cag act aaa cca cgc gaa acc cag tat aat agt aca tac cga       3027
His Ala Gln Thr Lys Pro Arg Glu Thr Gln Tyr Asn Ser Thr Tyr Arg           
    350                 355                 360                           

gtc gtg tca gtc ctg aca gtg act cac cag gat tgg ctg aac ggc aag       3075
Val Val Ser Val Leu Thr Val Thr His Gln Asp Trp Leu Asn Gly Lys           
365                 370                 375                 380           

gag tat acc tgc aag gtg tct aac aag gcc ctg ccc gcc cct atc cag       3123
Glu Tyr Thr Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Gln           
                385                 390                 395               

aaa aca att agc aag gac aaa ggg cag cca cgg gaa ccc cag gtg tac       3171
Lys Thr Ile Ser Lys Asp Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr           
            400                 405                 410                   

act ctg cca ccc tca aga gag gaa ctg act aag aac cag gtc agc ctg       3219
Thr Leu Pro Pro Ser Arg Glu Glu Leu Thr Lys Asn Gln Val Ser Leu           
        415                 420                 425                       

acc tgt ctg gtg aaa ggc ttc tac ccc agc gat atc gtc gtg gag tgg       3267
Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Val Val Glu Trp           
    430                 435                 440                           

gaa agt tca ggc cag cct gag aat act tac aag act acc cct cca gtg       3315
Glu Ser Ser Gly Gln Pro Glu Asn Thr Tyr Lys Thr Thr Pro Pro Val           
445                 450                 455                 460           

ctg gat agc gac ggg tcc tat ttc ctg tac agc aag ctg aca gtg gac       3363
Leu Asp Ser Asp Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Thr Val Asp           
                465                 470                 475               

aaa tcc cgc tgg cag cag gga aac gtc ttt tcc tgt tct gtg atg cat       3411
Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His           
            480                 485                 490                   

gag gcc ctg cac aat cat tac acc cag aag agt ctg tca ctg agc ccc       3459
Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro           
        495                 500                 505                       

ggc aaa tgaggtacct ctagagtcga cccgggcggc ctcgaggacg gggtgaacta        3515
Gly Lys                                                                   
    510                                                                   

cgcctgagga tccgatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct     3575

tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa     3635

ttttttgtgt ctctcactcg gaagcaattc gttgatctga atttcgacca cccataatac     3695

ccattaccct ggtagataag tagcatggcg ggttaatcat taactacaag gaacccctag     3755

tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa     3815

aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagcc     3875

ttaattaacc taattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg     3935

ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag     3995

aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc     4055

cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac     4115

ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg     4175

ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt     4235

tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc     4295

cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct     4355

tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga     4415

ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga     4475

attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg     4535

aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata     4595

accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg     4655

tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac     4715

gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact     4775

ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat     4835

gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga     4895

gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac     4955

agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat     5015

gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac     5075

cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct     5135

gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac     5195

gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga     5255

ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg     5315

gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact     5375

ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac     5435

tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta     5495

actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt     5555

taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga     5615

gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc     5675

tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt     5735

ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc     5795

gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc     5855

tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg     5915

cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg     5975

gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga     6035

actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc     6095

ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg     6155

gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg     6215

atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt     6275

tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc     6335

tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg     6395

aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc     6455

gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg     6515

gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca     6575

ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt     6635

tcacacagga aacagctatg accatgatta cgccagattt aattaaggcc ttaattagg      6694


<210>  8
<211>  510
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  8

Met Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Leu Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Glu Val Gln Leu Leu Glu Ser Gly Pro Gly Leu Val Lys 
            20                  25                  30          


Pro Ser Glu Thr Leu Ser Leu Thr Cys Ala Val Ser Gly Leu Ser Ile 
        35                  40                  45              


Ser Ser Asp Phe Ser Trp Ala Trp Ile Arg Gln Thr Pro Gly Lys Ala 
    50                  55                  60                  


Leu Glu Tyr Val Gly Tyr Ile Arg Gly Asn Thr Gly Asp Thr Tyr Tyr 
65                  70                  75                  80  


Asn Pro Ser Leu Lys Ser Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys 
                85                  90                  95      


Asn Gln Ile Tyr Leu Asn Leu Ser Ser Val Thr Ala Gly Asp Ala Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg Asp Arg Val Cys Asp Asp Asp Tyr Gly Tyr 
        115                 120                 125             


Tyr Tyr Thr Glu Val Cys Phe Gly Leu Asp Ser Trp Gly Gln Gly Ile 
    130                 135                 140                 


Val Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Gly Gly Gly Gly Ser Ala Glu Leu Val Met Thr Gln Ser Pro Leu Ser 
                165                 170                 175     


Leu Ser Val Ala Pro Gly Gln Thr Ala Ser Ile Ser Cys Arg Ser Ser 
            180                 185                 190         


Gln Ser Leu Asp Tyr Ala Asn Gly Asn Thr Tyr Leu Ser Trp Phe His 
        195                 200                 205             


Gln Arg Pro Gly Gln Pro Pro Arg Arg Leu Ile Tyr Gln Ile Ser Asn 
    210                 215                 220                 


Arg Asp Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ala Gly Thr 
225                 230                 235                 240 


Glu Phe Thr Leu Arg Ile Ser Arg Met Glu Ser Asp Asp Val Gly Ile 
                245                 250                 255     


Tyr Tyr Cys Gly Gln Gly Thr Thr Phe Pro Arg Thr Phe Gly Gln Gly 
            260                 265                 270         


Thr Lys Val Glu Ile Lys Thr Cys Gly Gly Gly Ser Lys Pro Pro Thr 
        275                 280                 285             


Cys Pro Pro Cys Thr Ser Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 
    290                 295                 300                 


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ala Ser Arg Thr Pro 
305                 310                 315                 320 


Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Asp Val 
                325                 330                 335     


Lys Phe Asn Trp Tyr Val Asn Gly Ala Glu Val His His Ala Gln Thr 
            340                 345                 350         


Lys Pro Arg Glu Thr Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
        355                 360                 365             


Leu Thr Val Thr His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Thr Cys 
    370                 375                 380                 


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Gln Lys Thr Ile Ser 
385                 390                 395                 400 


Lys Asp Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
                405                 410                 415     


Ser Arg Glu Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
            420                 425                 430         


Lys Gly Phe Tyr Pro Ser Asp Ile Val Val Glu Trp Glu Ser Ser Gly 
        435                 440                 445             


Gln Pro Glu Asn Thr Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
    450                 455                 460                 


Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
465                 470                 475                 480 


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
                485                 490                 495     


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
            500                 505                 510 


<210>  9
<211>  6694
<212>  DNA
<213>  Artificial sequence

<220>
<223>  engineered plasmid containining 201IA(H435) mutant


<220>
<221>  misc_feature
<222>  (275)..(404)
<223>  3' ITR (complement)

<220>
<221>  rep_origin
<222>  (2198)..(2786)

<220>
<221>  misc_feature
<222>  (3226)..(3355)

<220>
<221>  misc_feature
<222>  (3423)..(3804)
<223>  CMV IE promoter

<220>
<221>  promoter
<222>  (3807)..(4088)
<223>  CB promoter

<220>
<221>  TATA_signal
<222>  (4061)..(4064)
<223>  rabbit globin polyA

<220>
<221>  Intron
<222>  (4183)..(5155)
<223>  chicken beta-actin intron

<220>
<221>  CDS
<222>  (5161)..(6690)
<223>  CMV IE promoter

<400>  9
gtacctctag agtcgacccg ggcggcctcg aggacggggt gaactacgcc tgaggatccg       60

atctttttcc ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt      120

ctggctaata aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct      180

cactcggaag caattcgttg atctgaattt cgaccaccca taatacccat taccctggta      240

gataagtagc atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc      300

actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc      360

ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagccttaa ttaacctaat      420

tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat      480

cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat      540

cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca      600

ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta      660

gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt      720

caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac      780

cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt      840

tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga      900

acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg      960

gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata     1020

ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt     1080

tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc     1140

ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc     1200

ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa     1260

aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg     1320

gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag     1380

ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc     1440

gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta     1500

cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg     1560

cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca     1620

acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac     1680

caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat     1740

taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg     1800

ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata     1860

aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta     1920

agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa     1980

atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag     2040

tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg     2100

tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact     2160

gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg     2220

taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc     2280

aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata     2340

ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta     2400

catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc     2460

ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg     2520

ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac     2580

agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg     2640

taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt     2700

atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct     2760

cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg     2820

ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata     2880

accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca     2940

gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc     3000

gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg     3060

agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta     3120

tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca     3180

gctatgacca tgattacgcc agatttaatt aaggccttaa ttaggctgcg cgctcgctcg     3240

ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg cccggcctca     3300

gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg ttccttgtag     3360

ttaatgatta acccgccatg ctacttatct accagggtaa tggggatcct ctagaactat     3420

agctagtcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt     3480

tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg     3540

accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc     3600

aatagggact ttccattgac gtcaatgggt ggactattta cggtaaactg cccacttggc     3660

agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg     3720

gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat     3780

ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct     3840

ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg     3900

tgcagcgatg ggggcggggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga     3960

ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg     4020

aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg     4080

gcgggcgggg agtcgctgcg acgctgcctt cgccccgtgc cccgctccgc cgccgcctcg     4140

cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc     4200

ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg     4260

ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg     4320

ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc     4380

tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc     4440

gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg     4500

cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc     4560

cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt     4620

acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg     4680

ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg     4740

agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc     4800

gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc     4860

cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg     4920

ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg     4980

ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc     5040

tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc     5100

ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc     5160

atg gag ttc ggg ctg agc tgg gtc ttt ctg gtg gcc ctg ctg aag gga       5208
Met Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Leu Leu Lys Gly           
1               5                   10                  15                

gtc cag tgc gag gtg cag ctg ctg gaa tcc gga cct ggc ctg gtg aaa       5256
Val Gln Cys Glu Val Gln Leu Leu Glu Ser Gly Pro Gly Leu Val Lys           
            20                  25                  30                    

cca tct gag aca ctg agt ctg act tgt gct gtc tcc ggc ctg tct atc       5304
Pro Ser Glu Thr Leu Ser Leu Thr Cys Ala Val Ser Gly Leu Ser Ile           
        35                  40                  45                        

agc tcc gat ttc tcc tgg gca tgg att agg cag acc ccc ggc aag gcc       5352
Ser Ser Asp Phe Ser Trp Ala Trp Ile Arg Gln Thr Pro Gly Lys Ala           
    50                  55                  60                            

ctg gaa tat gtg ggg tac atc cgc ggg aac acc gga gat aca tac tat       5400
Leu Glu Tyr Val Gly Tyr Ile Arg Gly Asn Thr Gly Asp Thr Tyr Tyr           
65                  70                  75                  80            

aat cct agt ctg aag tca agg ctg act atc tca aag gac acc agc aaa       5448
Asn Pro Ser Leu Lys Ser Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys           
                85                  90                  95                

aac caa atc tac ctg aat ctg tct agt gtc acc gct ggc gat gcc gcc       5496
Asn Gln Ile Tyr Leu Asn Leu Ser Ser Val Thr Ala Gly Asp Ala Ala           
            100                 105                 110                   

gtg tac tat tgc gca agg gac cgg gtg tgc gac gat gac tac gga tac       5544
Val Tyr Tyr Cys Ala Arg Asp Arg Val Cys Asp Asp Asp Tyr Gly Tyr           
        115                 120                 125                       

tat tac acc gag gtg tgc ttc ggc ctg gat tct tgg ggg cag gga atc       5592
Tyr Tyr Thr Glu Val Cys Phe Gly Leu Asp Ser Trp Gly Gln Gly Ile           
    130                 135                 140                           

gtg gtc aca gtg tca agc ggc gga gga ggc agc gga gga gga ggg tcc       5640
Val Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser           
145                 150                 155                 160           

gga ggc ggg gga tct gca gaa ctg gtc atg aca cag tcc cca ctg agc       5688
Gly Gly Gly Gly Ser Ala Glu Leu Val Met Thr Gln Ser Pro Leu Ser           
                165                 170                 175               

ctg tcc gtc gct cca gga cag act gca tct att agt tgt cga tcc tct       5736
Leu Ser Val Ala Pro Gly Gln Thr Ala Ser Ile Ser Cys Arg Ser Ser           
            180                 185                 190                   

cag tcc ctg gac tat gct aac ggc aat acc tac ctg tct tgg ttt cac       5784
Gln Ser Leu Asp Tyr Ala Asn Gly Asn Thr Tyr Leu Ser Trp Phe His           
        195                 200                 205                       

cag cga cca gga cag cca cct cgg aga ctg atc tat cag att tcc aac       5832
Gln Arg Pro Gly Gln Pro Pro Arg Arg Leu Ile Tyr Gln Ile Ser Asn           
    210                 215                 220                           

aga gat tct gga gtg ccc gac agg ttc tca ggc agc gga gca gga act       5880
Arg Asp Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ala Gly Thr           
225                 230                 235                 240           

gag ttt acc ctg cga atc agt cgg atg gaa tca gat gac gtg ggg atc       5928
Glu Phe Thr Leu Arg Ile Ser Arg Met Glu Ser Asp Asp Val Gly Ile           
                245                 250                 255               

tac tac tgc gga cag ggg acc aca ttc cca cgg aca ttt gga cag ggc       5976
Tyr Tyr Cys Gly Gln Gly Thr Thr Phe Pro Arg Thr Phe Gly Gln Gly           
            260                 265                 270                   

act aag gtg gag atc aaa acc tgt gga gga gga agc aag cca cca acc       6024
Thr Lys Val Glu Ile Lys Thr Cys Gly Gly Gly Ser Lys Pro Pro Thr           
        275                 280                 285                       

tgc cct cca tgt aca tct ccc gaa ctg ctg ggc ggg cct agc gtg ttc       6072
Cys Pro Pro Cys Thr Ser Pro Glu Leu Leu Gly Gly Pro Ser Val Phe           
    290                 295                 300                           

ctg ttt ccc cct aag cct aaa gat aca ctg atg att agt aga acc cca       6120
Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro           
305                 310                 315                 320           

gag gtc aca tgc gtg gtc gtg gac gtg tcc cag gaa gat cct gac gtg       6168
Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Asp Val           
                325                 330                 335               

aag ttc aac tgg tac gtg aat ggc gcc gag gtg cac cat gct cag act       6216
Lys Phe Asn Trp Tyr Val Asn Gly Ala Glu Val His His Ala Gln Thr           
            340                 345                 350                   

aaa cca cgc gaa acc cag tat aat agt aca tac cga gtc gtg tca gtc       6264
Lys Pro Arg Glu Thr Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val           
        355                 360                 365                       

ctg aca gtg act cac cag gat tgg ctg aac ggc aag gag tat acc tgc       6312
Leu Thr Val Thr His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Thr Cys           
    370                 375                 380                           

aag gtg tct aac aag gcc ctg ccc gcc cct atc cag aaa aca att agc       6360
Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Gln Lys Thr Ile Ser           
385                 390                 395                 400           

aag gac aaa ggg cag cca cgg gaa ccc cag gtg tac act ctg cca ccc       6408
Lys Asp Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro           
                405                 410                 415               

tca aga gag gaa ctg act aag aac cag gtc agc ctg acc tgt ctg gtg       6456
Ser Arg Glu Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val           
            420                 425                 430                   

aaa ggc ttc tac ccc agc gat atc gtc gtg gag tgg gaa agt tca ggc       6504
Lys Gly Phe Tyr Pro Ser Asp Ile Val Val Glu Trp Glu Ser Ser Gly           
        435                 440                 445                       

cag cct gag aat act tac aag act acc cct cca gtg ctg gat agc gac       6552
Gln Pro Glu Asn Thr Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp           
    450                 455                 460                           

ggg tcc tat ttc ctg tac agc aag ctg aca gtg gac aaa tcc cgc tgg       6600
Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp           
465                 470                 475                 480           

cag cag gga aac gtc ttt tcc tgt tct gtg atg cat gag gcc ctg cac       6648
Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His           
                485                 490                 495               

aat gct tac acc cag aag agt ctg tca ctg agc ccc ggc aaa tgag          6694
Asn Ala Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys                   
            500                 505                 510                   


<210>  10
<211>  510
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  10

Met Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Leu Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Glu Val Gln Leu Leu Glu Ser Gly Pro Gly Leu Val Lys 
            20                  25                  30          


Pro Ser Glu Thr Leu Ser Leu Thr Cys Ala Val Ser Gly Leu Ser Ile 
        35                  40                  45              


Ser Ser Asp Phe Ser Trp Ala Trp Ile Arg Gln Thr Pro Gly Lys Ala 
    50                  55                  60                  


Leu Glu Tyr Val Gly Tyr Ile Arg Gly Asn Thr Gly Asp Thr Tyr Tyr 
65                  70                  75                  80  


Asn Pro Ser Leu Lys Ser Arg Leu Thr Ile Ser Lys Asp Thr Ser Lys 
                85                  90                  95      


Asn Gln Ile Tyr Leu Asn Leu Ser Ser Val Thr Ala Gly Asp Ala Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg Asp Arg Val Cys Asp Asp Asp Tyr Gly Tyr 
        115                 120                 125             


Tyr Tyr Thr Glu Val Cys Phe Gly Leu Asp Ser Trp Gly Gln Gly Ile 
    130                 135                 140                 


Val Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
145                 150                 155                 160 


Gly Gly Gly Gly Ser Ala Glu Leu Val Met Thr Gln Ser Pro Leu Ser 
                165                 170                 175     


Leu Ser Val Ala Pro Gly Gln Thr Ala Ser Ile Ser Cys Arg Ser Ser 
            180                 185                 190         


Gln Ser Leu Asp Tyr Ala Asn Gly Asn Thr Tyr Leu Ser Trp Phe His 
        195                 200                 205             


Gln Arg Pro Gly Gln Pro Pro Arg Arg Leu Ile Tyr Gln Ile Ser Asn 
    210                 215                 220                 


Arg Asp Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ala Gly Thr 
225                 230                 235                 240 


Glu Phe Thr Leu Arg Ile Ser Arg Met Glu Ser Asp Asp Val Gly Ile 
                245                 250                 255     


Tyr Tyr Cys Gly Gln Gly Thr Thr Phe Pro Arg Thr Phe Gly Gln Gly 
            260                 265                 270         


Thr Lys Val Glu Ile Lys Thr Cys Gly Gly Gly Ser Lys Pro Pro Thr 
        275                 280                 285             


Cys Pro Pro Cys Thr Ser Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 
    290                 295                 300                 


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
305                 310                 315                 320 


Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Asp Val 
                325                 330                 335     


Lys Phe Asn Trp Tyr Val Asn Gly Ala Glu Val His His Ala Gln Thr 
            340                 345                 350         


Lys Pro Arg Glu Thr Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
        355                 360                 365             


Leu Thr Val Thr His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Thr Cys 
    370                 375                 380                 


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Gln Lys Thr Ile Ser 
385                 390                 395                 400 


Lys Asp Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
                405                 410                 415     


Ser Arg Glu Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
            420                 425                 430         


Lys Gly Phe Tyr Pro Ser Asp Ile Val Val Glu Trp Glu Ser Ser Gly 
        435                 440                 445             


Gln Pro Glu Asn Thr Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
    450                 455                 460                 


Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
465                 470                 475                 480 


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
                485                 490                 495     


Asn Ala Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
            500                 505                 510 


<210>  11
<211>  7284
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Plasmid containing the heavy and light chains of trastuzumab


<220>
<221>  misc_feature
<222>  (1)..(130)
<223>  5' ITR

<220>
<221>  promoter
<222>  (191)..(932)
<223>  human CMV IE enhancer and promoter

<220>
<221>  TATA_signal
<222>  (897)..(901)

<220>
<221>  Intron
<222>  (1047)..(1179)

<220>
<221>  sig_peptide
<222>  (1305)..(1364)
<223>  IL-2 signal peptide

<220>
<221>  CDS
<222>  (1365)..(1724)
<223>  Trastuzumab heavy variable

<220>
<221>  CDS
<222>  (1725)..(2720)
<223>  trastuzumab heavy constant

<220>
<221>  enhancer
<222>  (2726)..(3313)
<223>  IRES

<220>
<221>  sig_peptide
<222>  (3314)..(3373)
<223>  IL2 signal peptide

<220>
<221>  CDS
<222>  (3374)..(3694)
<223>  trastuzumab light variable

<220>
<221>  CDS
<222>  (3695)..(4021)
<223>  trastuzumab constant light (kappa)

<220>
<221>  polyA_signal
<222>  (4038)..(4269)
<223>  SV40 late polyA signal

<220>
<221>  polyA_signal
<222>  (4083)..(4269)
<223>  SV40 late polyA signal

<220>
<221>  misc_feature
<222>  (4334)..(4463)
<223>  3' ITR (complement)

<220>
<221>  rep_origin
<222>  (4640)..(5095))

<220>
<221>  CDS
<222>  (5226)..(6083)
<223>  amp-R

<220>
<221>  misc_feature
<222>  (6257)..(6845)
<223>  COL/E1/origin

<400>  11
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact      120

aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct      180

aggaagatct tcaatattgg ccattagcca tattattcat tggttatata gcataaatca      240

atattggcta ttggccattg catacgttgt atctatatca taatatgtac atttatattg      300

gctcatgtcc aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat      360

caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg      420

taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt      480

atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac      540

ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg      600

acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact      660

ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt      720

ggcagtacac caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc      780

ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc      840

gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata      900

taagcagagc tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc      960

acagttaaat tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca     1020

gaagttggtc gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag     1080

accaatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta     1140

ttggtcttac tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt     1200

acagctctta aggctagagt acttaatacg actcactata ggctagcggg gactttgcac     1260

tggaacttac aacacccgag caaggacgcg actctagacc caccatgtac cggatgcagc     1320

tgctgagctg tatcgccctg tctctggccc tcgtgaccaa cagc gaa gtg cag ctg      1376
                                                 Glu Val Gln Leu          
                                                 1                        

gtg gaa agc ggc gga gga ctg gtg cag cct ggc gga tct ctg aga ctg       1424
Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu           
5                   10                  15                  20            

agc tgt gcc gcc agc ggc ttc aac atc aag gac acc tac atc cac tgg       1472
Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys Asp Thr Tyr Ile His Trp           
                25                  30                  35                

gtg cgc cag gcc cct ggc aag gga ctg gaa tgg gtg gcc aga atc tac       1520
Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala Arg Ile Tyr           
            40                  45                  50                    

ccc acc aac ggc tac acc aga tac gcc gac agc gtg aag ggc cgg ttc       1568
Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser Val Lys Gly Arg Phe           
        55                  60                  65                        

acc atc agc gcc gac acc agc aag aac acc gcc tac ctg cag atg aac       1616
Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr Leu Gln Met Asn           
    70                  75                  80                            

agc ctg cgg gcc gag gac acc gcc gtg tac tac tgt agt aga tgg gga       1664
Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ser Arg Trp Gly           
85                  90                  95                  100           

ggc gac ggc ttc tac gcc atg gac tat tgg ggc cag ggc acc ctc gtg       1712
Gly Asp Gly Phe Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Leu Val           
                105                 110                 115               

aca gtg tct agt gcg tcg acc aag ggg ccc tcg gtc ttc ccc ctg gca       1760
Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala           
            120                 125                 130                   

ccc tcc tcc aag agc acc tct ggg ggc aca gcg gcc ctg ggc tgc ctg       1808
Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu           
        135                 140                 145                       

gtc aag gac tac ttc ccc gaa ccg gtg acg gtg tcg tgg aac tca ggc       1856
Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly           
    150                 155                 160                           

gcc ctg acc agc ggc gtg cac acc ttc ccg gct gtc cta cag tcc tca       1904
Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser           
165                 170                 175                 180           

gga ctc tac tcc ctc agc agc gtg gtg acc gtg ccc tcc agc agc ttg       1952
Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu           
                185                 190                 195               

ggc acc cag acc tac atc tgc aac gtg aat cac aag ccc agc aac acc       2000
Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr           
            200                 205                 210                   

aag gtg gac aag aaa gtt gag ccc aaa tct tgt gac aaa act cac aca       2048
Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr           
        215                 220                 225                       

tgc cca ccg tgc cca gca cct gaa ctc ctg ggg gga ccg tca gtc ttc       2096
Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe           
    230                 235                 240                           

ctc ttc ccc cca aaa ccc aag gac acc ctc atg atc tcc cgg acc cct       2144
Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro           
245                 250                 255                 260           

gag gtc aca tgc gtg gtg gtg gac gtg agc cac gaa gac cct gag gtc       2192
Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val           
                265                 270                 275               

aag ttc aac tgg tac gtg gac ggc gtg gag gtg cat aat gcc aag aca       2240
Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr           
            280                 285                 290                   

aag ccg cgg gag gag cag tac aac agc acg tac cgt gtg gtc agc gtc       2288
Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val           
        295                 300                 305                       

ctc acc gtc ctg cac cag gac tgg ctg aat ggc aag gag tac aag tgc       2336
Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys           
    310                 315                 320                           

aag gtc tcc aac aaa gcc ctc cca gcc ccc atc gag aaa acc atc tcc       2384
Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser           
325                 330                 335                 340           

aaa gcc aaa ggg cag ccc cga gaa cca cag gtg tac acc ctg ccc cca       2432
Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro           
                345                 350                 355               

tcc cgg gat gag ctg acc aag aac cag gtc agc ctg acc tgc ctg gtc       2480
Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val           
            360                 365                 370                   

aaa ggc ttc tat ccc agc gac atc gcc gtg gag tgg gag agc aat ggg       2528
Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly           
        375                 380                 385                       

cag ccg gag aac aac tac aag acc acg cct ccc gtg ctg gac tcc gac       2576
Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp           
    390                 395                 400                           

ggc tcc ttc ttc ctc tac agc aag ctc acc gtg gac aag agc agg tgg       2624
Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp           
405                 410                 415                 420           

cag cag ggg aac gtc ttc tca tgc tcc gtg atg cat gag gct ctg cac       2672
Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His           
                425                 430                 435               

aac cac tac acg cag aag agc ctc tcc ctg tct ccc ggc aag tga taa       2720
Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys                   
            440                 445                 450                   

ggccggcccc tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg     2780

ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag     2840

ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc     2900

caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg     2960

aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag     3020

gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca     3080

gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt     3140

caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc     3200

tcggtacaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa     3260

ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca accatgtacc     3320

gcatgcaact cctgtcttgc attgcactaa gtcttgcact tgtcacaaac agt gat        3376
                                                           Asp            
                                                                          

atc cag atg acc cag agc ccc agc agc ctg tct gcc agc gtg ggc gac       3424
Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp           
            455                 460                 465                   

aga gtg acc atc acc tgt aga gcc agc cag gac gtg aac acc gcc gtg       3472
Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala Val           
        470                 475                 480                       

gcc tgg tat cag cag aag cct ggc aag gcc ccc aag ctg ctg atc tac       3520
Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr           
    485                 490                 495                           

agc gcc agc ttc ctg tac agc ggc gtg ccc agc aga ttc agc ggc agc       3568
Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly Ser           
500                 505                 510                 515           

aga tcc ggc acc gac ttc acc ctg acc atc agc tcc ctg cag ccc gag       3616
Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu           
                520                 525                 530               

gac ttc gcc acc tac tac tgc cag cag cac tac acc acc ccc ccc aca       3664
Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro Thr           
            535                 540                 545                   

ttt ggc cag ggc acc aag gtg gaa atc aag cgt acg gtg gct gca cca       3712
Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Thr Val Ala Ala Pro           
        550                 555                 560                       

tct gtc ttc atc ttc ccg cca tct gat gag cag ttg aaa tct gga act       3760
Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr           
    565                 570                 575                           

gcc tct gtt gtg tgc ctg ctg aat aac ttc tac ccc aga gaa gcc aaa       3808
Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys           
580                 585                 590                 595           

gtg cag tgg aag gtg gac aac gcc ctg cag agc gga aac agc cag gaa       3856
Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu           
                600                 605                 610               

agc gtg aca gag cag gat tcc aag gat tcc aca tac agc ctg agc agc       3904
Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser           
            615                 620                 625                   

aca ctg aca ctg tcc aag gcc gac tac gag aag cac aag gtg tac gcc       3952
Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala           
        630                 635                 640                       

tgc gaa gtg aca cac cag gga ctg tcc tcc cct gtg aca aag agc ttc       4000
Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe           
    645                 650                 655                           

aac aga gga gaa tgc tga tga aagcttgcgg ccgcttcgag cagacatgat          4051
Asn Arg Gly Glu Cys                                                       
660                                                                       

aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat     4111

ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt     4171

taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt     4231

ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatcttc ctagagcatg     4291

gctacgtaga taagtagcat ggcgggttaa tcattaacta caaggaaccc ctagtgatgg     4351

agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg     4411

cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agccttaatt     4471

aacctaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc     4531

aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc     4591

gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta     4651

gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca     4711

gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct     4771

ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc     4831

acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat     4891

agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc     4951

aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc     5011

cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta     5071

acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc     5131

tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg     5191

ataaatgctt caataatatt gaaaaaggaa gagt atg agt att caa cat ttc cgt     5246
                                      Met Ser Ile Gln His Phe Arg         
                                      665                 670             

gtc gcc ctt att ccc ttt ttt gcg gca ttt tgc ctt cct gtt ttt gct       5294
Val Ala Leu Ile Pro Phe Phe Ala Ala Phe Cys Leu Pro Val Phe Ala           
            675                 680                 685                   

cac cca gaa acg ctg gtg aaa gta aaa gat gct gaa gat cag ttg ggt       5342
His Pro Glu Thr Leu Val Lys Val Lys Asp Ala Glu Asp Gln Leu Gly           
        690                 695                 700                       

gca cga gtg ggt tac atc gaa ctg gat ctc aac agc ggt aag atc ctt       5390
Ala Arg Val Gly Tyr Ile Glu Leu Asp Leu Asn Ser Gly Lys Ile Leu           
    705                 710                 715                           

gag agt ttt cgc ccc gaa gaa cgt ttt cca atg atg agc act ttt aaa       5438
Glu Ser Phe Arg Pro Glu Glu Arg Phe Pro Met Met Ser Thr Phe Lys           
720                 725                 730                 735           

gtt ctg cta tgt ggc gcg gta tta tcc cgt att gac gcc ggg caa gag       5486
Val Leu Leu Cys Gly Ala Val Leu Ser Arg Ile Asp Ala Gly Gln Glu           
                740                 745                 750               

caa ctc ggt cgc cgc ata cac tat tct cag aat gac ttg gtt gag tac       5534
Gln Leu Gly Arg Arg Ile His Tyr Ser Gln Asn Asp Leu Val Glu Tyr           
            755                 760                 765                   

tca cca gtc aca gaa aag cat ctt acg gat ggc atg aca gta aga gaa       5582
Ser Pro Val Thr Glu Lys His Leu Thr Asp Gly Met Thr Val Arg Glu           
        770                 775                 780                       

tta tgc agt gct gcc ata acc atg agt gat aac act gcg gcc aac tta       5630
Leu Cys Ser Ala Ala Ile Thr Met Ser Asp Asn Thr Ala Ala Asn Leu           
    785                 790                 795                           

ctt ctg aca acg atc gga gga ccg aag gag cta acc gct ttt ttg cac       5678
Leu Leu Thr Thr Ile Gly Gly Pro Lys Glu Leu Thr Ala Phe Leu His           
800                 805                 810                 815           

aac atg ggg gat cat gta act cgc ctt gat cgt tgg gaa ccg gag ctg       5726
Asn Met Gly Asp His Val Thr Arg Leu Asp Arg Trp Glu Pro Glu Leu           
                820                 825                 830               

aat gaa gcc ata cca aac gac gag cgt gac acc acg atg cct gta gca       5774
Asn Glu Ala Ile Pro Asn Asp Glu Arg Asp Thr Thr Met Pro Val Ala           
            835                 840                 845                   

atg gca aca acg ttg cgc aaa cta tta act ggc gaa cta ctt act cta       5822
Met Ala Thr Thr Leu Arg Lys Leu Leu Thr Gly Glu Leu Leu Thr Leu           
        850                 855                 860                       

gct tcc cgg caa caa tta ata gac tgg atg gag gcg gat aaa gtt gca       5870
Ala Ser Arg Gln Gln Leu Ile Asp Trp Met Glu Ala Asp Lys Val Ala           
    865                 870                 875                           

gga cca ctt ctg cgc tcg gcc ctt ccg gct ggc tgg ttt att gct gat       5918
Gly Pro Leu Leu Arg Ser Ala Leu Pro Ala Gly Trp Phe Ile Ala Asp           
880                 885                 890                 895           

aaa tct gga gcc ggt gag cgt ggg tct cgc ggt atc att gca gca ctg       5966
Lys Ser Gly Ala Gly Glu Arg Gly Ser Arg Gly Ile Ile Ala Ala Leu           
                900                 905                 910               

ggg cca gat ggt aag ccc tcc cgt atc gta gtt atc tac acg acg ggg       6014
Gly Pro Asp Gly Lys Pro Ser Arg Ile Val Val Ile Tyr Thr Thr Gly           
            915                 920                 925                   

agt cag gca act atg gat gaa cga aat aga cag atc gct gag ata ggt       6062
Ser Gln Ala Thr Met Asp Glu Arg Asn Arg Gln Ile Ala Glu Ile Gly           
        930                 935                 940                       

gcc tca ctg att aag cat tgg taactgtcag accaagttta ctcatatata          6113
Ala Ser Leu Ile Lys His Trp                                               
    945                 950                                               

ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt     6173

gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc     6233

gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg     6293

caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact     6353

ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg     6413

tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg     6473

ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac     6533

tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca     6593

cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga     6653

gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc     6713

ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct     6773

gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg     6833

agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct     6893

tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc     6953

tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc     7013

gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat     7073

taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt     7133

aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt     7193

atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat     7253

tacgccagat ttaattaagg ccttaattag g                                    7284


<210>  12
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  12

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys Asp Thr 
            20                  25                  30          


Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser 
        115                 120 


<210>  13
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  13

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  14
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  14

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105         


<210>  15
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  15

Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210>  16
<211>  286
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  16

Met Ser Ile Gln His Phe Arg Val Ala Leu Ile Pro Phe Phe Ala Ala 
1               5                   10                  15      


Phe Cys Leu Pro Val Phe Ala His Pro Glu Thr Leu Val Lys Val Lys 
            20                  25                  30          


Asp Ala Glu Asp Gln Leu Gly Ala Arg Val Gly Tyr Ile Glu Leu Asp 
        35                  40                  45              


Leu Asn Ser Gly Lys Ile Leu Glu Ser Phe Arg Pro Glu Glu Arg Phe 
    50                  55                  60                  


Pro Met Met Ser Thr Phe Lys Val Leu Leu Cys Gly Ala Val Leu Ser 
65                  70                  75                  80  


Arg Ile Asp Ala Gly Gln Glu Gln Leu Gly Arg Arg Ile His Tyr Ser 
                85                  90                  95      


Gln Asn Asp Leu Val Glu Tyr Ser Pro Val Thr Glu Lys His Leu Thr 
            100                 105                 110         


Asp Gly Met Thr Val Arg Glu Leu Cys Ser Ala Ala Ile Thr Met Ser 
        115                 120                 125             


Asp Asn Thr Ala Ala Asn Leu Leu Leu Thr Thr Ile Gly Gly Pro Lys 
    130                 135                 140                 


Glu Leu Thr Ala Phe Leu His Asn Met Gly Asp His Val Thr Arg Leu 
145                 150                 155                 160 


Asp Arg Trp Glu Pro Glu Leu Asn Glu Ala Ile Pro Asn Asp Glu Arg 
                165                 170                 175     


Asp Thr Thr Met Pro Val Ala Met Ala Thr Thr Leu Arg Lys Leu Leu 
            180                 185                 190         


Thr Gly Glu Leu Leu Thr Leu Ala Ser Arg Gln Gln Leu Ile Asp Trp 
        195                 200                 205             


Met Glu Ala Asp Lys Val Ala Gly Pro Leu Leu Arg Ser Ala Leu Pro 
    210                 215                 220                 


Ala Gly Trp Phe Ile Ala Asp Lys Ser Gly Ala Gly Glu Arg Gly Ser 
225                 230                 235                 240 


Arg Gly Ile Ile Ala Ala Leu Gly Pro Asp Gly Lys Pro Ser Arg Ile 
                245                 250                 255     


Val Val Ile Tyr Thr Thr Gly Ser Gln Ala Thr Met Asp Glu Arg Asn 
            260                 265                 270         


Arg Gln Ile Ala Glu Ile Gly Ala Ser Leu Ile Lys His Trp 
        275                 280                 285     


<210>  17
<211>  4440
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Engineered plasmid containing trastuzumab MAb


<220>
<221>  sig_peptide
<222>  (1254)..(1313)
<223>  IL2 signal sequence

<220>
<221>  CDS
<222>  (1314)..(1673)
<223>  Trastuzamab heavy variable

<220>
<221>  CDS
<222>  (1674)..(2660)
<223>  Trastuzamab heavy constant

<220>
<221>  sig_peptide
<222>  (2745)..(2804)
<223>  IL2 signal sequence

<220>
<221>  CDS
<222>  (2805)..(3110)
<223>  Trastuzamab light variable

<220>
<221>  CDS
<222>  (3111)..(3452)
<223>  Trastuzamab light constant (kappa)

<400>  17
ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt       60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact      120

aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct      180

aggaagatct tcaatattgg ccattagcca tattattcat tggttatata gcataaatca      240

atattggcta ttggccattg catacgttgt atctatatca taatatgtac atttatattg      300

gctcatgtcc aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat      360

caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg      420

taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt      480

atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac      540

ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg      600

acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact      660

ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt      720

ggcagtacac caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc      780

ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc      840

gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata      900

taagcagagc tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc      960

acagttaaat tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca     1020

gaagttggtc gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag     1080

accaatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta     1140

ttggtcttac tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt     1200

acagctctta aggctagagt acttaatacg actcactata ggctagaccc accatgtacc     1260

ggatgcagct gctgagctgt atcgccctgt ctctggccct cgtgaccaac agc gaa        1316
                                                           Glu            
                                                           1              

gtg cag ctg gtg gaa agc ggc gga gga ctg gtg cag cct ggc gga tct       1364
Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser           
            5                   10                  15                    

ctg aga ctg agc tgt gcc gcc agc ggc ttc aac atc aag gac acc tac       1412
Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys Asp Thr Tyr           
        20                  25                  30                        

atc cac tgg gtg cgc cag gcc cct ggc aag gga ctg gaa tgg gtg gcc       1460
Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val Ala           
    35                  40                  45                            

aga atc tac ccc acc aac ggc tac acc aga tac gcc gac agc gtg aag       1508
Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser Val Lys           
50                  55                  60                  65            

ggc cgg ttc acc atc agc gcc gac acc agc aag aac acc gcc tac ctg       1556
Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr Leu           
                70                  75                  80                

cag atg aac agc ctg cgg gcc gag gac acc gcc gtg tac tac tgt agt       1604
Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ser           
            85                  90                  95                    

aga tgg gga ggc gac ggc ttc tac gcc atg gac tat tgg ggc cag ggc       1652
Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr Trp Gly Gln Gly           
        100                 105                 110                       

acc ctc gtg aca gtg tct agt gcg tcg acc aag gga cct tcg gtc ttc       1700
Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe           
    115                 120                 125                           

ccc ctg gca ccc tcc tcc aag agc acc tct ggg ggc aca gcg gcc ctg       1748
Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu           
130                 135                 140                 145           

ggc tgc ctg gtc aag gac tac ttc ccc gaa ccg gtg acg gtg tcg tgg       1796
Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp           
                150                 155                 160               

aac tca ggc gcc ctg acc agc ggc gtg cac acc ttc ccg gct gtc cta       1844
Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu           
            165                 170                 175                   

cag tcc tca gga ctc tac tcc ctc agc agc gtg gtg acc gtg ccc tcc       1892
Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser           
        180                 185                 190                       

agc agc ttg ggc acc cag acc tac atc tgc aac gtg aat cac aag ccc       1940
Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro           
    195                 200                 205                           

agc aac acc aag gtg gac aag aaa gtt gaa cca aag agc tgc gac aag       1988
Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys           
210                 215                 220                 225           

acc cac acg tgt ccc ccc tgc cct gcc cct gaa ctg ctg gga ggc ccc       2036
Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro           
                230                 235                 240               

agc gtg ttc ctg ttc ccc cca aag ccc aag gac acc ctg atg atc agc       2084
Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser           
            245                 250                 255                   

cgg acc ccc gaa gtg acc tgc gtg gtg gtg gac gtg tcc cac gag gac       2132
Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp           
        260                 265                 270                       

cct gaa gtg aag ttt aat tgg tac gtg gac ggc gtg gaa gtg cac aac       2180
Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn           
    275                 280                 285                           

gcc aag acc aag ccc aga gag gaa cag tac aac agc acc tac cgg gtg       2228
Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val           
290                 295                 300                 305           

gtg tcc gtg ctg acc gtg ctg cac cag gac tgg ctg aac ggc aaa gag       2276
Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu           
                310                 315                 320               

tac aag tgc aag gtg tcc aac aag gcc ctg cct gcc ccc atc gag aaa       2324
Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys           
            325                 330                 335                   

acc atc agc aag gcc aag ggc cag ccc cgc gag cct cag gtc tac aca       2372
Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr           
        340                 345                 350                       

ctg ccc ccc agc cgg gaa gag atg acc aag aac cag gtg tcc ctg acc       2420
Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr           
    355                 360                 365                           

tgc ctg gtc aag ggc ttc tac ccc agc gac atc gcc gtg gaa tgg gag       2468
Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu           
370                 375                 380                 385           

agc aac ggc cag ccc gag aac aac tac aag acc acc ccc cct gtg ctg       2516
Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu           
                390                 395                 400               

gac agc gac ggc tca ttc ttc ctg tat agc aag ctg acc gtg gac aag       2564
Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys           
            405                 410                 415                   

agc cgg tgg cag cag ggc aac gtg ttc agc tgc agc gtg atg cac gag       2612
Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu           
        420                 425                 430                       

gcc ctg cac aac cac tac acc cag aag tcc ctg agc ctg agc ccc ggc       2660
Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly           
    435                 440                 445                           

agaaagcgga gagcccccgt gaagcagacc ctgaacttcg acctgctgaa gctggccggc     2720

gacgtggaaa gcaaccctgg ccctatgtac agaatgcagc tgctgctgct gatcgccctg     2780

agcctggccc tggtgaccaa cagc gat atc cag atg acc cag agc ccc agc        2831
                           Asp Ile Gln Met Thr Gln Ser Pro Ser            
                           450                 455                        

agc ctg tct gcc agc gtg ggc gac aga gtg acc atc acc tgt aga gcc       2879
Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr Cys Arg Ala           
    460                 465                 470                           

agc cag gac gtg aac acc gcc gtg gcc tgg tat cag cag aag cct ggc       2927
Ser Gln Asp Val Asn Thr Ala Val Ala Trp Tyr Gln Gln Lys Pro Gly           
475                 480                 485                 490           

aag gcc ccc aag ctg ctg atc tac agc gcc agc ttc ctg tac agc ggc       2975
Lys Ala Pro Lys Leu Leu Ile Tyr Ser Ala Ser Phe Leu Tyr Ser Gly           
                495                 500                 505               

gtg ccc agc aga ttc agc ggc agc aga tcc ggc acc gac ttc acc ctg       3023
Val Pro Ser Arg Phe Ser Gly Ser Arg Ser Gly Thr Asp Phe Thr Leu           
            510                 515                 520                   

acc atc agc tcc ctg cag ccc gag gac ttc gcc acc tac tac tgc cag       3071
Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln           
        525                 530                 535                       

cag cac tac acc acc ccc ccc aca ttt ggc cag ggc acc aag gtg gaa       3119
Gln His Tyr Thr Thr Pro Pro Thr Phe Gly Gln Gly Thr Lys Val Glu           
    540                 545                 550                           

atc aag cgt acg gtg gcc gcc cca agc gtg ttc atc ttc cca cca agc       3167
Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser           
555                 560                 565                 570           

gat gag cag ctg aag agc gga acc gcc agc gtg gtg tgc ctg ctg aac       3215
Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn           
                575                 580                 585               

aac ttc tac cca cgg gag gcc aag gtg cag tgg aag gtg gat aac gcc       3263
Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala           
            590                 595                 600                   

ctg cag agc gga aac agc cag gag agc gtg acc gag cag gat agc aag       3311
Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys           
        605                 610                 615                       

gat agc acc tac agc ctg agc agc acc ctg acc ctg agc aag gcc gat       3359
Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp           
    620                 625                 630                           

tac gag aag cac aag gtg tac gcc tgc gag gtg acc cac cag gga ctg       3407
Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu           
635                 640                 645                 650           

agc agc cca gtg acc aag agc ttc aac cgc gga gag tgc tga taa           3452
Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys                       
                655                 660                                   

agcggccgct tcgagcagac atgataagat acattgatga gtttggacaa accacaacta     3512

gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa     3572

ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg     3632

ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa     3692

tcgataagga tcttcctaga gcatggctac gtagataagt agcatggcgg gttaatcatt     3752

aactacaagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc     3812

actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg     3872

agcgagcgag cgcgcagcct taattaacct aattcactgg ccgtcgtttt acaacgtcgt     3932

gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc     3992

agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg     4052

aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg     4112

cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct     4172

tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta     4232

gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt     4292

tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg     4352

ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat     4412

tcttttgatt tataagggat tttgccga                                        4440


<210>  18
<211>  120
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  18

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn Ile Lys Asp Thr 
            20                  25                  30          


Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser 
        115                 120 


<210>  19
<211>  329
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  19

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
225                 230                 235                 240 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly 
                325                 


<210>  20
<211>  102
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  20

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Asn Thr Ala 
            20                  25                  30          


Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Arg Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln His Tyr Thr Thr Pro Pro 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr 
            100         


<210>  21
<211>  112
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  21

Lys Val Glu Ile Lys Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe 
1               5                   10                  15      


Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys 
            20                  25                  30          


Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val 
        35                  40                  45              


Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln 
    50                  55                  60                  


Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser 
65                  70                  75                  80  


Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala Cys Glu Val Thr His 
                85                  90                  95      


Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105                 110         


<210>  22
<211>  12
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Peptide mimotope of HER2 epitope

<400>  22

Leu Leu Gly Pro Tyr Glu Leu Trp Glu Leu Ser His 
1               5                   10          


<210>  23
<211>  689
<212>  PRT
<213>  Artificial sequence

<220>
<223>  trastuzumab immunoglobulin H435A mutant

<400>  23

Gly Ala Gly Cys Cys Cys Ala Ala Ala Thr Cys Thr Thr Gly Thr Gly 
1               5                   10                  15      


Ala Cys Ala Ala Ala Ala Cys Thr Cys Ala Cys Ala Cys Ala Thr Gly 
            20                  25                  30          


Cys Cys Cys Ala Cys Cys Gly Thr Gly Cys Cys Cys Ala Gly Cys Ala 
        35                  40                  45              


Cys Cys Thr Gly Ala Ala Cys Thr Cys Cys Thr Gly Gly Gly Gly Gly 
    50                  55                  60                  


Gly Ala Cys Cys Gly Thr Cys Ala Gly Thr Cys Thr Thr Cys Cys Thr 
65                  70                  75                  80  


Cys Thr Thr Cys Cys Cys Cys Cys Cys Ala Ala Ala Ala Cys Cys Cys 
                85                  90                  95      


Ala Ala Gly Gly Ala Cys Ala Cys Cys Cys Thr Cys Ala Thr Gly Ala 
            100                 105                 110         


Thr Cys Thr Cys Cys Cys Gly Gly Ala Cys Cys Cys Cys Thr Gly Ala 
        115                 120                 125             


Gly Gly Thr Cys Ala Cys Ala Thr Gly Cys Gly Thr Gly Gly Thr Gly 
    130                 135                 140                 


Gly Thr Gly Gly Ala Cys Gly Thr Gly Ala Gly Cys Cys Ala Cys Gly 
145                 150                 155                 160 


Ala Ala Gly Ala Cys Cys Cys Thr Gly Ala Gly Gly Thr Cys Ala Ala 
                165                 170                 175     


Gly Thr Thr Cys Ala Ala Cys Thr Gly Gly Thr Ala Cys Gly Thr Gly 
            180                 185                 190         


Gly Ala Cys Gly Gly Cys Gly Thr Gly Gly Ala Gly Gly Thr Gly Cys 
        195                 200                 205             


Ala Thr Ala Ala Thr Gly Cys Cys Ala Ala Gly Ala Cys Ala Ala Ala 
    210                 215                 220                 


Gly Cys Cys Gly Cys Gly Gly Gly Ala Gly Gly Ala Gly Cys Ala Gly 
225                 230                 235                 240 


Thr Ala Cys Ala Ala Cys Ala Gly Cys Ala Cys Gly Thr Ala Cys Cys 
                245                 250                 255     


Gly Thr Gly Thr Gly Gly Thr Cys Ala Gly Cys Gly Thr Cys Cys Thr 
            260                 265                 270         


Cys Ala Cys Cys Gly Thr Cys Cys Thr Gly Cys Ala Cys Cys Ala Gly 
        275                 280                 285             


Gly Ala Cys Thr Gly Gly Cys Thr Gly Ala Ala Thr Gly Gly Cys Ala 
    290                 295                 300                 


Ala Gly Gly Ala Gly Thr Ala Cys Ala Ala Gly Thr Gly Cys Ala Ala 
305                 310                 315                 320 


Gly Gly Thr Cys Thr Cys Cys Ala Ala Cys Ala Ala Ala Gly Cys Cys 
                325                 330                 335     


Cys Thr Cys Cys Cys Ala Gly Cys Cys Cys Cys Cys Ala Thr Cys Gly 
            340                 345                 350         


Ala Gly Ala Ala Ala Ala Cys Cys Ala Thr Cys Thr Cys Cys Ala Ala 
        355                 360                 365             


Ala Gly Cys Cys Ala Ala Ala Gly Gly Gly Cys Ala Gly Cys Cys Cys 
    370                 375                 380                 


Cys Gly Ala Gly Ala Ala Cys Cys Ala Cys Ala Gly Gly Thr Gly Thr 
385                 390                 395                 400 


Ala Cys Ala Cys Cys Cys Thr Gly Cys Cys Cys Cys Cys Ala Thr Cys 
                405                 410                 415     


Cys Cys Gly Gly Gly Ala Thr Gly Ala Gly Cys Thr Gly Ala Cys Cys 
            420                 425                 430         


Ala Ala Gly Ala Ala Cys Cys Ala Gly Gly Thr Cys Ala Gly Cys Cys 
        435                 440                 445             


Thr Gly Ala Cys Cys Thr Gly Cys Cys Thr Gly Gly Thr Cys Ala Ala 
    450                 455                 460                 


Ala Gly Gly Cys Thr Thr Cys Thr Ala Thr Cys Cys Cys Ala Gly Cys 
465                 470                 475                 480 


Gly Ala Cys Ala Thr Cys Gly Cys Cys Gly Thr Gly Gly Ala Gly Thr 
                485                 490                 495     


Gly Gly Gly Ala Gly Ala Gly Cys Ala Ala Thr Gly Gly Gly Cys Ala 
            500                 505                 510         


Gly Cys Cys Gly Gly Ala Gly Ala Ala Cys Ala Ala Cys Thr Ala Cys 
        515                 520                 525             


Ala Ala Gly Ala Cys Cys Ala Cys Gly Cys Cys Thr Cys Cys Cys Gly 
    530                 535                 540                 


Thr Gly Cys Thr Gly Gly Ala Cys Thr Cys Cys Gly Ala Cys Gly Gly 
545                 550                 555                 560 


Cys Thr Cys Cys Thr Thr Cys Thr Thr Cys Cys Thr Cys Thr Ala Cys 
                565                 570                 575     


Ala Gly Cys Ala Ala Gly Cys Thr Cys Ala Cys Cys Gly Thr Gly Gly 
            580                 585                 590         


Ala Cys Ala Ala Gly Ala Gly Cys Ala Gly Gly Thr Gly Gly Cys Ala 
        595                 600                 605             


Gly Cys Ala Gly Gly Gly Gly Ala Ala Cys Gly Thr Cys Thr Thr Cys 
    610                 615                 620                 


Thr Cys Ala Thr Gly Cys Thr Cys Cys Gly Thr Gly Ala Thr Gly Cys 
625                 630                 635                 640 


Ala Thr Gly Ala Gly Gly Cys Thr Cys Thr Gly Cys Ala Cys Ala Ala 
                645                 650                 655     


Cys Gly Cys Cys Thr Ala Cys Ala Cys Gly Cys Ala Gly Ala Ala Gly 
            660                 665                 670         


Ala Gly Cys Cys Thr Cys Thr Cys Cys Cys Thr Gly Thr Cys Thr Cys 
        675                 680                 685             


Cys 
    


<210>  24
<211>  689
<212>  DNA
<213>  Artificial sequence

<220>
<223>  immunoglobulin fragment with I253A mutant

<400>  24
gagcccaaat cttgtgacaa aactcacaca tgcccaccgt gcccagcacc tgaactcctg       60

gggggaccgt cagtcttcct cttcccccca aaacccaagg acaccctcat ggcctcccgg      120

acccctgagg tcacatgcgt ggtggtggac gtgagccacg aagaccctga ggtcaagttc      180

aactggtacg tggacggcgt ggaggtgcat aatgccaaga caaagccgcg ggaggagcag      240

tacaacagca cgtaccgtgt ggtcagcgtc ctcaccgtcc tgcaccagga ctggctgaat      300

ggcaaggagt acaagtgcaa ggtctccaac aaagccctcc cagcccccat cgagaaaacc      360

atctccaaag ccaaagggca gccccgagaa ccacaggtgt acaccctgcc cccatcccgg      420

gatgagctga ccaagaacca ggtcagcctg acctgcctgg tcaaaggctt ctatcccagc      480

gacatcgccg tggagtggga gagcaatggg cagccggaga acaactacaa gaccacgcct      540

cccgtgctgg actccgacgg ctccttcttc ctctacagca agctcaccgt ggacaagagc      600

aggtggcagc aggggaacgt cttctcatgc tccgtgatgc atgaggctct gcacaaccac      660

tacacgcaga agagcctctc cctgtctcc                                        689


<210>  25
<211>  469
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Trastruzumab heavy chain

<400>  25

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val 
            20                  25                  30          


Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Asn 
        35                  40                  45              


Ile Lys Asp Thr Tyr Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly 
    50                  55                  60                  


Leu Glu Trp Val Ala Arg Ile Tyr Pro Thr Asn Gly Tyr Thr Arg Tyr 
65                  70                  75                  80  


Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys 
                85                  90                  95      


Asn Thr Ala Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ser Arg Trp Gly Gly Asp Gly Phe Tyr Ala Met Asp 
        115                 120                 125             


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys 
    130                 135                 140                 


Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly 
145                 150                 155                 160 


Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro 
                165                 170                 175     


Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr 
            180                 185                 190         


Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val 
        195                 200                 205             


Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn 
    210                 215                 220                 


Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Lys Val Glu Pro 
225                 230                 235                 240 


Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 
                245                 250                 255     


Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
            260                 265                 270         


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
        275                 280                 285             


Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly 
    290                 295                 300                 


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn 
305                 310                 315                 320 


Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
                325                 330                 335     


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro 
            340                 345                 350         


Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
        355                 360                 365             


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn 
    370                 375                 380                 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
385                 390                 395                 400 


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
                405                 410                 415     


Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
            420                 425                 430         


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
        435                 440                 445             


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
    450                 455                 460                 


Ser Leu Ser Pro Gly 
465                 


