                         SEQUENCE LISTING

<110>  DSM IP Assets B.V.
 
<120>  STABILIZATION OF CYTOCHROME P450 REDUCTASE

<130>  30270-WO-PCT

<150>  EP14165949.0
<151>  2014-04-25

<160>  35    

<170>  PatentIn version 3.5

<210>  1
<211>  1476
<212>  DNA
<213>  Saccharomyces cerevisiae


<220>
<221>  CDS
<222>  (1)..(1476)

<400>  1
atg acc agt ttg tcc aaa agc ttc atg cag agt gga cga atc tgc gca         48
Met Thr Ser Leu Ser Lys Ser Phe Met Gln Ser Gly Arg Ile Cys Ala           
1               5                   10                  15                

gca tgt ttc tat ctg tta ttc aca cta ctt tca att cca atc tcg ttt         96
Ala Cys Phe Tyr Leu Leu Phe Thr Leu Leu Ser Ile Pro Ile Ser Phe           
            20                  25                  30                    

aaa gtt ggt ggt ttg gaa tgc ggg ctt tcc ttc acg gtg aca ctg ttc        144
Lys Val Gly Gly Leu Glu Cys Gly Leu Ser Phe Thr Val Thr Leu Phe           
        35                  40                  45                        

act tta tat ttc ata act acg act ctt aac gtg ttg gca aga cga cat        192
Thr Leu Tyr Phe Ile Thr Thr Thr Leu Asn Val Leu Ala Arg Arg His           
    50                  55                  60                            

gga gga aga cta tac att ttt ttt acc aac tgt ctg tat tac tca caa        240
Gly Gly Arg Leu Tyr Ile Phe Phe Thr Asn Cys Leu Tyr Tyr Ser Gln           
65                  70                  75                  80            

cat ttt atc att gca tct ttg cta tac ctg ttt ttg tct gga ttt tct        288
His Phe Ile Ile Ala Ser Leu Leu Tyr Leu Phe Leu Ser Gly Phe Ser           
                85                  90                  95                

aat gat gag ttg gga aac gtt ctg aaa aat aaa tat aat gag tcg gag        336
Asn Asp Glu Leu Gly Asn Val Leu Lys Asn Lys Tyr Asn Glu Ser Glu           
            100                 105                 110                   

tcg ttc ctg gaa gct ttg aaa aat agc ttg aat tcc aat caa att aac        384
Ser Phe Leu Glu Ala Leu Lys Asn Ser Leu Asn Ser Asn Gln Ile Asn           
        115                 120                 125                       

tac gtc tta tat tat tac tac tat cga ttt gtt gta caa ccg tgg caa        432
Tyr Val Leu Tyr Tyr Tyr Tyr Tyr Arg Phe Val Val Gln Pro Trp Gln           
    130                 135                 140                           

ttc gtg ctt acc aag tcc aca cct ttt ttt act cta tcg gaa ggt ttt        480
Phe Val Leu Thr Lys Ser Thr Pro Phe Phe Thr Leu Ser Glu Gly Phe           
145                 150                 155                 160           

ttc act att tta gcc att cag gcc gtc ggg gaa act aat aga tgg tta        528
Phe Thr Ile Leu Ala Ile Gln Ala Val Gly Glu Thr Asn Arg Trp Leu           
                165                 170                 175               

tca aat gac ttg aat tca aac acg tgg att att tcc tca ttg tta acc        576
Ser Asn Asp Leu Asn Ser Asn Thr Trp Ile Ile Ser Ser Leu Leu Thr           
            180                 185                 190                   

tcc gga ggt gtg att acc gca tcg ctg tac tat ttg tat cgg att tat        624
Ser Gly Gly Val Ile Thr Ala Ser Leu Tyr Tyr Leu Tyr Arg Ile Tyr           
        195                 200                 205                       

gtc acc ccc ata tgg ccg tta tcc atc caa acg gcg tcc tta tta gga        672
Val Thr Pro Ile Trp Pro Leu Ser Ile Gln Thr Ala Ser Leu Leu Gly           
    210                 215                 220                           

ctt gtt ttg tct atg gta tgt gga ctg ggg ttg tat ggt att gtg agt        720
Leu Val Leu Ser Met Val Cys Gly Leu Gly Leu Tyr Gly Ile Val Ser           
225                 230                 235                 240           

caa aaa gga tcc gtc ata gag agc tct tta ttt ttt gcg tat att gtt        768
Gln Lys Gly Ser Val Ile Glu Ser Ser Leu Phe Phe Ala Tyr Ile Val           
                245                 250                 255               

cgt tgt att tat gaa att tcc ccc aaa tta gct act acc gcg act gat        816
Arg Cys Ile Tyr Glu Ile Ser Pro Lys Leu Ala Thr Thr Ala Thr Asp           
            260                 265                 270                   

gaa att tta aat ttg ttc aaa gac gtc tgg cag aaa cat caa aga aat        864
Glu Ile Leu Asn Leu Phe Lys Asp Val Trp Gln Lys His Gln Arg Asn           
        275                 280                 285                       

ctg ccc aca gct gac aat ctt ttg tgc tac ttt cat aat gtc ata ttg        912
Leu Pro Thr Ala Asp Asn Leu Leu Cys Tyr Phe His Asn Val Ile Leu           
    290                 295                 300                           

aaa aat gca gag gtg tta tgg ggg tcc ttt att cct aga gga aga aag        960
Lys Asn Ala Glu Val Leu Trp Gly Ser Phe Ile Pro Arg Gly Arg Lys           
305                 310                 315                 320           

aaa acc ggt gat ttt cat gat aaa ctc att agc att cta tca ttc gaa       1008
Lys Thr Gly Asp Phe His Asp Lys Leu Ile Ser Ile Leu Ser Phe Glu           
                325                 330                 335               

aaa gta tcc ttg ata tct aaa cca ttt tgg aaa ttt ttc aag aat ttc       1056
Lys Val Ser Leu Ile Ser Lys Pro Phe Trp Lys Phe Phe Lys Asn Phe           
            340                 345                 350                   

acc ttt agt gtt ccg cta tcc att aat gaa ttt tgt caa gtt aca att       1104
Thr Phe Ser Val Pro Leu Ser Ile Asn Glu Phe Cys Gln Val Thr Ile           
        355                 360                 365                       

aag atg gca agc gaa tca gtt tcc cca gct ata gta atc aat tta tgc       1152
Lys Met Ala Ser Glu Ser Val Ser Pro Ala Ile Val Ile Asn Leu Cys           
    370                 375                 380                           

ttt aga gtt ctg atg ttt tac tcg gca acg agg att att cca gca tta       1200
Phe Arg Val Leu Met Phe Tyr Ser Ala Thr Arg Ile Ile Pro Ala Leu           
385                 390                 395                 400           

caa aga aaa aat gac aaa cag ttg cgc aag agt cgc agg atc atg aag       1248
Gln Arg Lys Asn Asp Lys Gln Leu Arg Lys Ser Arg Arg Ile Met Lys           
                405                 410                 415               

gga ttg tat tgg tac agt cct tgc ata tta att gct atg tat act cac       1296
Gly Leu Tyr Trp Tyr Ser Pro Cys Ile Leu Ile Ala Met Tyr Thr His           
            420                 425                 430                   

ctg att tta caa tat tca ggt gag cta aag aaa gac ctg tgc ata tgg       1344
Leu Ile Leu Gln Tyr Ser Gly Glu Leu Lys Lys Asp Leu Cys Ile Trp           
        435                 440                 445                       

ggt tgc agt gaa aag tgg ttt ggc gta gat caa cca gaa att ata gta       1392
Gly Cys Ser Glu Lys Trp Phe Gly Val Asp Gln Pro Glu Ile Ile Val           
    450                 455                 460                           

gat tca tgg gga ttt tgg aac tgg tgc aac att ttc tgt act att ttg       1440
Asp Ser Trp Gly Phe Trp Asn Trp Cys Asn Ile Phe Cys Thr Ile Leu           
465                 470                 475                 480           

gta tac gct aca gaa tta ata ggt tct ggt agt tga                       1476
Val Tyr Ala Thr Glu Leu Ile Gly Ser Gly Ser                               
                485                 490                                   


<210>  2
<211>  491
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  2

Met Thr Ser Leu Ser Lys Ser Phe Met Gln Ser Gly Arg Ile Cys Ala 
1               5                   10                  15      


Ala Cys Phe Tyr Leu Leu Phe Thr Leu Leu Ser Ile Pro Ile Ser Phe 
            20                  25                  30          


Lys Val Gly Gly Leu Glu Cys Gly Leu Ser Phe Thr Val Thr Leu Phe 
        35                  40                  45              


Thr Leu Tyr Phe Ile Thr Thr Thr Leu Asn Val Leu Ala Arg Arg His 
    50                  55                  60                  


Gly Gly Arg Leu Tyr Ile Phe Phe Thr Asn Cys Leu Tyr Tyr Ser Gln 
65                  70                  75                  80  


His Phe Ile Ile Ala Ser Leu Leu Tyr Leu Phe Leu Ser Gly Phe Ser 
                85                  90                  95      


Asn Asp Glu Leu Gly Asn Val Leu Lys Asn Lys Tyr Asn Glu Ser Glu 
            100                 105                 110         


Ser Phe Leu Glu Ala Leu Lys Asn Ser Leu Asn Ser Asn Gln Ile Asn 
        115                 120                 125             


Tyr Val Leu Tyr Tyr Tyr Tyr Tyr Arg Phe Val Val Gln Pro Trp Gln 
    130                 135                 140                 


Phe Val Leu Thr Lys Ser Thr Pro Phe Phe Thr Leu Ser Glu Gly Phe 
145                 150                 155                 160 


Phe Thr Ile Leu Ala Ile Gln Ala Val Gly Glu Thr Asn Arg Trp Leu 
                165                 170                 175     


Ser Asn Asp Leu Asn Ser Asn Thr Trp Ile Ile Ser Ser Leu Leu Thr 
            180                 185                 190         


Ser Gly Gly Val Ile Thr Ala Ser Leu Tyr Tyr Leu Tyr Arg Ile Tyr 
        195                 200                 205             


Val Thr Pro Ile Trp Pro Leu Ser Ile Gln Thr Ala Ser Leu Leu Gly 
    210                 215                 220                 


Leu Val Leu Ser Met Val Cys Gly Leu Gly Leu Tyr Gly Ile Val Ser 
225                 230                 235                 240 


Gln Lys Gly Ser Val Ile Glu Ser Ser Leu Phe Phe Ala Tyr Ile Val 
                245                 250                 255     


Arg Cys Ile Tyr Glu Ile Ser Pro Lys Leu Ala Thr Thr Ala Thr Asp 
            260                 265                 270         


Glu Ile Leu Asn Leu Phe Lys Asp Val Trp Gln Lys His Gln Arg Asn 
        275                 280                 285             


Leu Pro Thr Ala Asp Asn Leu Leu Cys Tyr Phe His Asn Val Ile Leu 
    290                 295                 300                 


Lys Asn Ala Glu Val Leu Trp Gly Ser Phe Ile Pro Arg Gly Arg Lys 
305                 310                 315                 320 


Lys Thr Gly Asp Phe His Asp Lys Leu Ile Ser Ile Leu Ser Phe Glu 
                325                 330                 335     


Lys Val Ser Leu Ile Ser Lys Pro Phe Trp Lys Phe Phe Lys Asn Phe 
            340                 345                 350         


Thr Phe Ser Val Pro Leu Ser Ile Asn Glu Phe Cys Gln Val Thr Ile 
        355                 360                 365             


Lys Met Ala Ser Glu Ser Val Ser Pro Ala Ile Val Ile Asn Leu Cys 
    370                 375                 380                 


Phe Arg Val Leu Met Phe Tyr Ser Ala Thr Arg Ile Ile Pro Ala Leu 
385                 390                 395                 400 


Gln Arg Lys Asn Asp Lys Gln Leu Arg Lys Ser Arg Arg Ile Met Lys 
                405                 410                 415     


Gly Leu Tyr Trp Tyr Ser Pro Cys Ile Leu Ile Ala Met Tyr Thr His 
            420                 425                 430         


Leu Ile Leu Gln Tyr Ser Gly Glu Leu Lys Lys Asp Leu Cys Ile Trp 
        435                 440                 445             


Gly Cys Ser Glu Lys Trp Phe Gly Val Asp Gln Pro Glu Ile Ile Val 
    450                 455                 460                 


Asp Ser Trp Gly Phe Trp Asn Trp Cys Asn Ile Phe Cys Thr Ile Leu 
465                 470                 475                 480 


Val Tyr Ala Thr Glu Leu Ile Gly Ser Gly Ser 
                485                 490     


<210>  3
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Fw_CPR_HindIII

<400>  3
cccaagcttc gaaacgcata tgacttctg                                         29


<210>  4
<211>  69
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Rv_CPR-myc_BamHI

<400>  4
cgcggatcct tattacaaat cctcttcaga aatcaatttt tgttcccaga catctctcaa       60

gtatctacc                                                               69


<210>  5
<211>  38
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: pYES2_AscI_fw

<400>  5
ttggcgcgcc ttaattaaac ggattagaag ccgccgag                               38


<210>  6
<211>  36
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: pYES2_BglII_rev

<400>  6
ttagatctgg cgcgcccgat tcattaatgc agggcc                                 36


<210>  7
<211>  29
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Fw_HPOSc_HindIII

<400>  7
cccaagcttc gaaacgcata tgcaattct                                         29


<210>  8
<211>  64
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Rv_HPO-Flag_Sc

<400>  8
cgcggatccc tcgagttatt acttatcgtc gtcatccttg taatcctctc gggaaggttg       60

gtaa                                                                    64


<210>  9
<211>  38
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: BglII_Gal1_fw

<400>  9
ttggcgcgcc ttaattaaac ggattagaag ccgccgag                               38


<210>  10
<211>  36
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: AscI_CYC1_rev

<400>  10
ttagatctgg cgcgcccgat tcattaatgc agggcc                                 36


<210>  11
<211>  60
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: F1(trp1)

<400>  11
gtgagtatac gtgattaagc acacaaaggc agcttggagt cggatccccg ggttaattaa       60


<210>  12
<211>  60
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: R1(trp1)

<400>  12
tgcacaaaca atacttaaat aaatactact cagtaataac gaattcgagc tcgtttaaac       60


<210>  13
<211>  32
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Fw(PPGK1_XmaI)

<400>  13
gtacccgggg attattttag attcctgact tc                                     32


<210>  14
<211>  33
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer:Rv(PPGK1_BamHI)

<400>  14
tatggatcct cttgttttta tatttgttgt aaa                                    33


<210>  15
<211>  38
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Fw(tHMG1_PstI)

<400>  15
gacctgcagg caccctgcag accaattggt gaaaactg                               38


<210>  16
<211>  36
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Rv(tHMG1_ HindIII)

<400>  16
tgcaagcttg gcctaacaca tggtgctgtt gtgctt                                 36


<210>  17
<211>  58
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Fw(ex_leu2_tHMG1)

<400>  17
agcaatatat atatatatat ttcaaggata taccattcta tgtaaaacga cggccagt         58


<210>  18
<211>  58
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Rv(ex_leu2_tHMG1)

<400>  18
taaagtttat gtacaaatat cataaaaaaa gagaatcttt ccgattcatt aatgcagc         58


<210>  19
<211>  55
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: F4 (ICE2)

<400>  19
cgtaaagtgt tggtggatct tatagtattc gtgaagaatt cgagctcgtt taaac            55


<210>  20
<211>  47
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: R2 (ICE2)

<400>  20
ctgcatgaag cttttggaca aactggtcat tttgagatcc gggtttt                     47


<210>  21
<211>  50
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: F1 (ICE2)

<400>  21
gtggccgatc acgctaaaga ttaggcaacg cggatccccg ggttaattaa                  50


<210>  22
<211>  60
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: R1 (ICE2)

<400>  22
gtatttcacc ttcctttttg tcttcgcgta tttggcaaag gaattcgagc tcgtttaaac       60


<210>  23
<211>  65
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: F1 (ICE2start)

<400>  23
agagaggtgc tgtttgtggc cgatcacgct aaagattagg caacgatgac cagtttgtcc       60

aaaag                                                                   65


<210>  24
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Fw(CDC73_qRT)

<400>  24
gaaaggcgag acatccgata                                                   20


<210>  25
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Rv(CDC73_qRT)

<400>  25
ttgtttccac cacaactgga                                                   20


<210>  26
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Fw(HPO_qRT)

<400>  26
acattgcgtt ttgcccttac                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Rv(HPO_qRT)

<400>  27
gcaacacttc atcgcgtcta                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Fw(CPR_qRT)

<400>  28
ggttgctggt ttcgttgtct                                                   20


<210>  29
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Rv(CPR_qRT)

<400>  29
acccaagtcc aagtcgtcat                                                   20


<210>  30
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Fw(ICE2_qRT)

<400>  30
cgtctggcag aaacatcaaa                                                   20


<210>  31
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Rv(ICE2_qRT)

<400>  31
aaggaccccc ataacacctc                                                   20


<210>  32
<211>  46
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Fw(CPR_NotI)

<400>  32
cctcactaaa gggcggccgc aacaaaatga cttctgcttt gtacgc                      46


<210>  33
<211>  40
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: Rv(CPRmyc_BglII)

<400>  33
ttaattaaga gctcagatct ttattaccag acatctctca                             40


<210>  34
<211>  33
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: FwIce2Pp

<400>  34
cggaattccg aaacgatgcc caagatacgc tcc                                    33


<210>  35
<211>  64
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Primer: RvIce2Pp

<400>  35
ataagaatgc ggccgcttag tgatggtgat ggtggtgtgt ctcccaatta ctagtcaaat       60

tatc                                                                    64


