                         SEQUENCE LISTING

<110>  TERACLON IDF, SL
       Paniagua-Sols, Jorge F.
       Elosua, Carolina
       Licea, Alexei
       Mata-Gonzlez, Mara -Teresa
       Olgun, Araceli
 
<120>  ANTICUERPOS VNAR RECOMBINANTES ANTI-VEGF

<130>  TERACLON-SILANES

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  368
<212>  DNA
<213>  Heterodontus francisci


<220>
<221>  V13
<222>  (1)..(368)

<220>
<221>  SEQ ID NO: 1
<222>  (1)..(368)

<400>  1
gcaagcctgg accaaacacc aagaacggca acgagagaga caggcgaatc cctgagcatt       60

aactgcgtcc tcactgatac tagccatatt ttgttcggca caaaatggct ctggaataat      120

ccgggttcaa cagattggga aagcataacg attggcggac gatatgctga atcagtcaac      180

aaccaagcaa agtcattttc tctgcaaatc aaggacctga cagttgaaga cagtggcacc      240

tattactgca agcgcaaacc ataggaagac gcaaaaatct acttccacgc ccattggtga      300

acggtatagc tgcgatgggg tatagctcca gtgactacga cggagctggc accgtgctga      360

ctgtgaac                                                               368


<210>  2
<211>  123
<212>  PRT
<213>  Heterodontus francisci


<220>
<221>  V13
<222>  (1)..(123)

<220>
<221>  SEQ ID NO : 2
<222>  (1)..(123)

<400>  2

Ala Ser Leu Asp Gln Thr Pro Arg Thr Ala Thr Arg Glu Thr Gly Glu 
1               5                   10                  15      


Ser Leu Ser Ile Asn Cys Val Leu Thr Asp Thr Ser His Ile Leu Phe 
            20                  25                  30          


Gly Thr Lys Trp Leu Trp Asn Asn Pro Gly Ser Thr Asp Trp Glu Ser 
        35                  40                  45              


Ile Thr Ile Gly Gly Arg Tyr Ala Glu Ser Val Asn Asn Gln Ala Lys 
    50                  55                  60                  


Ser Phe Ser Leu Gln Ile Lys Asp Leu Thr Val Glu Asp Ser Gly Thr 
65                  70                  75                  80  


Tyr Tyr Cys Lys Ala Gln Thr Ile Gly Arg Arg Lys Asn Leu Leu Pro 
                85                  90                  95      


Arg Pro Leu Val Asn Gly Ile Ala Ala Met Gly Tyr Ser Ser Ser Asp 
            100                 105                 110         


Tyr Asp Gly Ala Gly Thr Val Leu Thr Val Asn 
        115                 120             


<210>  3
<211>  339
<212>  DNA
<213>  Orectolobus maculatus


<220>
<221>  V19
<222>  (1)..(339)

<220>
<221>  SEQ ID NO: 3
<222>  (1)..(339)

<400>  3
caacgggttg aacaaacacc aagaacagca acaaaagaga cgggcgaatc actgaccatc       60

aactgcgtcc taagagatgc tagttttgaa ttaaaagaca cgggctggta tcggacaaaa      120

ttgggttcaa caaatgagca gagtatatca attggcggac gatatgttga aacagtcaac      180

aagggatcaa agtccttttc tctgagaatt agtgatctga gagttgaaga cagtggcacg      240

tataagtgtc aagcattcta ttctcttccg ttgggcgatt acaactattc tctgctgttt      300

aggggtgaga aaggagctgg caccgtgctg actgtgaac                             339


<210>  4
<211>  114
<212>  PRT
<213>  Orectolobus maculatus


<220>
<221>  V19
<222>  (1)..(114)

<220>
<221>  SEQ ID NO: 4
<222>  (1)..(114)

<400>  4

Ala Gln Arg Val Glu Gln Thr Pro Arg Thr Ala Thr Lys Glu Thr Gly 
1               5                   10                  15      


Glu Ser Leu Thr Ile Asn Cys Val Leu Arg Asp Ala Ser Phe Glu Leu 
            20                  25                  30          


Lys Asp Thr Gly Trp Tyr Arg Thr Lys Leu Gly Ser Thr Asn Glu Gln 
        35                  40                  45              


Ser Ile Ser Ile Gly Gly Arg Tyr Val Glu Thr Val Asn Lys Gly Ser 
    50                  55                  60                  


Lys Ser Phe Ser Leu Arg Ile Ser Asp Leu Arg Val Glu Asp Ser Gly 
65                  70                  75                  80  


Thr Tyr Lys Cys Gln Ala Phe Tyr Ser Leu Pro Leu Gly Asp Tyr Asn 
                85                  90                  95      


Tyr Ser Leu Leu Phe Arg Gly Glu Lys Gly Ala Gly Thr Val Leu Thr 
            100                 105                 110         


Val Asn 
        


<210>  5
<211>  342
<212>  DNA
<213>  Heterodontus francisci


<220>
<221>  V32R
<222>  (1)..(342)

<220>
<221>  SEQ ID NO: 5
<222>  (1)..(342)

<400>  5
gcaagcctgg accaaacacc aagaacggca acgagagaga cgggcgaatc cctgaccatt       60

aactgcgtct tcactgattc tagctgtggt ttgtgcggca catcttggtt ccggaataat      120

ccgggttcaa cagattggga acgcataacg attggcggac gatatgttga atcagtcaac      180

aagggagcaa agtcattttc tctgcaaatc aaggacctga cagttgaaga cagtgtcacc      240

tattactgca aagcgcaagg tcatcgatac ttcagtaagg tgtgcgagct gcgatgtccc      300

agttactact acgacggagc tggcaccgtg ctgactgtga ac                         342


<210>  6
<211>  115
<212>  PRT
<213>  Heterodontus francisci


<220>
<221>  V32R
<222>  (1)..(115)

<220>
<221>  SEQ ID NO: 6
<222>  (1)..(115)

<400>  6

Ala Ala Ser Leu Asp Gln Thr Pro Arg Thr Ala Thr Arg Glu Thr Gly 
1               5                   10                  15      


Glu Ser Leu Thr Ile Asn Cys Val Phe Thr Asp Ser Ser Cys Gly Leu 
            20                  25                  30          


Cys Gly Thr Ser Trp Phe Arg Asn Asn Pro Gly Ser Thr Asp Trp Glu 
        35                  40                  45              


Arg Ile Thr Ile Gly Gly Arg Tyr Val Glu Ser Val Asn Lys Gly Ala 
    50                  55                  60                  


Lys Ser Phe Ser Leu Gln Ile Lys Asp Leu Thr Val Glu Asp Ser Val 
65                  70                  75                  80  


Thr Tyr Tyr Cys Lys Ala Gln Gly His Arg Tyr Phe Ser Lys Val Cys 
                85                  90                  95      


Glu Leu Arg Cys Pro Ser Tyr Tyr Tyr Asp Gly Ala Gly Thr Val Leu 
            100                 105                 110         


Thr Val Asn 
        115 


<210>  7
<211>  94
<212>  PRT
<213>  Homo sapiens


<220>
<221>  A y B VEGF
<222>  (1)..(94)

<220>
<221>  SEQ ID NO: 7
<222>  (1)..(94)

<220>
<221>  VEGF: A y B
<222>  (1)..(94)

<400>  7

Val Val Lys Phe Met Asp Val Tyr Gln Arg Ser Tyr Cys His Pro Ile 
1               5                   10                  15      


Glu Thr Leu Val Asp Ile Phe Gln Glu Tyr Pro Asp Glu Ile Glu Tyr 
            20                  25                  30          


Ile Phe Lys Pro Ser Cys Val Pro Leu Met Arg Cys Gly Gly Cys Cys 
        35                  40                  45              


Asn Asp Glu Gly Leu Glu Cys Val Pro Thr Glu Glu Ser Asn Ile Thr 
    50                  55                  60                  


Met Gln Ile Met Arg Ile Lys Pro His Gln Gly Gln His Ile Gly Glu 
65                  70                  75                  80  


Met Ser Phe Leu Gln His Asn Lys Cys Glu Cys Arg Pro Lys 
                85                  90                  


<210>  8
<211>  113
<212>  PRT
<213>  Orectolobus maculatus


<220>
<221>  vNAR  V19
<222>  (1)..(113)

<220>
<221>  SEQ ID NO: 8
<222>  (1)..(113)

<400>  8

Ala Trp Val Asp Gln Thr Pro Arg Thr Ala Thr Lys Glu Thr Gly Glu 
1               5                   10                  15      


Ser Leu Thr Ile Asn Cys Val Leu Arg Asp Ala Ser Phe Glu Leu Lys 
            20                  25                  30          


Asp Thr Gly Trp Tyr Arg Thr Lys Leu Gly Ser Thr Asn Glu Gln Ser 
        35                  40                  45              


Ile Ser Ile Gly Gly Arg Tyr Val Glu Thr Val Asn Lys Gly Ser Lys 
    50                  55                  60                  


Ser Phe Ser Leu Arg Ile Ser Asp Leu Arg Val Glu Asp Ser Gly Thr 
65                  70                  75                  80  


Tyr Lys Cys Gln Ala Phe Tyr Ser Leu Pro Leu Gly Asp Tyr Asn Tyr 
                85                  90                  95      


Ser Leu Leu Phe Arg Gly Glu Lys Gly Ala Gly Thr Val Leu Thr Val 
            100                 105                 110         


Lys 
    


<210>  9
<211>  121
<212>  PRT
<213>  Heterodontus francisci


<220>
<221>  vNARV 32R
<222>  (1)..(121)

<220>
<221>  SEQ ID NO: 9
<222>  (1)..(121)

<400>  9

Met Ala Ala Ser Leu Asp Gln Thr Pro Arg Thr Ala Thr Arg Glu Thr 
1               5                   10                  15      


Gly Glu Ser Leu Thr Ile Asn Cys Val Phe Thr Asp Ser Ser Cys Gly 
            20                  25                  30          


Leu Cys Gly Thr Ser Trp Phe Arg Asn Asn Pro Gly Ser Thr Asp Trp 
        35                  40                  45              


Glu Arg Ile Thr Ile Gly Gly Arg Tyr Val Glu Ser Val Asn Lys Gly 
    50                  55                  60                  


Ala Lys Ser Phe Ser Leu Gln Ile Lys Asp Leu Thr Val Glu Asp Ser 
65                  70                  75                  80  


Val Thr Tyr Tyr Cys Lys Ala Gln Gly His Arg Tyr Phe Ser Lys Val 
                85                  90                  95      


Cys Glu Leu Arg Cys Pro Ser Tyr Tyr Tyr Asp Gly Ala Gly Thr Val 
            100                 105                 110         


Leu Thr Val Asn Gly Gln Ala Gly Gln 
        115                 120     


<210>  10
<211>  26
<212>  DNA
<213>  Elasmobranchii


<220>
<221>  IgNAR cds parcial
<222>  (1)..(26)

<220>
<221>  SEQ ID NO: 10
<222>  (1)..(26)

<400>  10
gttcacagtc agcacggtgc cagctc                                            26


<210>  11
<211>  20
<212>  DNA
<213>  Desconocido


<220>
<221>  Oligonucletido sentido No.1
<222>  (1)..(20)

<220>
<221>  SEQ ID NO: 11
<222>  (1)..(20)
<223>  Secuencia artificial

<400>  11
caacgggttg aacaaacacc                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Desconocida


<220>
<221>  Oligonucletido sentido No.2
<222>  (1)..(20)

<220>
<221>  SEQ ID NO: 12
<222>  (1)..(20)
<223>  Secuancia artificial

<400>  12
caacgggttg aacaaacacc                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Desconocida


<220>
<221>  Oligonucletido sentido No.3
<222>  (1)..(20)

<220>
<221>  SEQ ID NO: 13
<222>  (1)..(20)
<223>  Secuencia artificial

<400>  13
acaagggtag accaaacacc                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Desconocida


<220>
<221>  Oligonucletido sentido No.4
<222>  (1)..(20)

<220>
<221>  SEQ ID NO: 14
<222>  (1)..(20)

<220>
<221>  seq id no: 14
<222>  (1)..(20)
<223>  Secuencia artificial

<400>  14
gcaagggtgg accaaacacc                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Desconocida


<220>
<221>  Oligonucletido sentido No.5
<222>  (1)..(20)

<220>
<221>  SEQ ID NO: 15
<222>  (1)..(20)
<223>  Secuencia artificial

<400>  15
gcatgggtag accaaacacc                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Desconocida


<220>
<221>  Oligonucletido sentido No.6
<222>  (1)..(20)

<220>
<221>  SEQ ID NO: 16
<222>  (1)..(20)
<223>  Secuencia artificial

<400>  16
gcaagcctgg accaaacacc                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Desconocida


<220>
<221>  Oligonucletido sentido No.7
<222>  (1)..(20)

<220>
<221>  SEQ ID NO: 17
<222>  (1)..(20)

<400>  17
gcattgacgg accaaacacc                                                   20


