
                                SEQUENCE LISTING

<110> Seattle Children's Hospital
      d/b/a Seattle Children's Research Institute
      Jensen, Michael C.

<120> METHOD AND COMPOSITIONS FOR CELLULAR
  IMMUNOTHERAPY

<130> SCRI.065WO

<150> 62/090,845          
<151> 2014-12-11  

<150> 62/089,730          
<151> 2014-12-09  

<150> 62/088,363          
<151> 2014-12-05  

<150> 62/058,973          
<151> 2014-10-02  

<150> 61/986,479          
<151> 2014-04-30  

<150> 61/977,751          
<151> 2014-04-10  

<160> 60

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> Spacer Region

<220> 
<221> VARIANT        
<222> 1
<223> Xaa = cysteine, glycine, or arginine

<220> 
<221> VARIANT        
<222> 4
<223> Xaa = cysteine or threonine

<400> 1
Xaa Pro Pro Xaa Pro
 1               5  


<210> 2

<400> 2
 000

<210> 3

<400> 3
 000

<210> 4

<400> 4
 000

<210> 5
<211> 34
<212> PRT
<213> Artificial Sequence

<220> 
<223> CD28 transmembrane domain polynucleotide

<400> 5
Pro Pro Cys Pro Pro Cys Pro Met Phe Trp Val Leu Val Val Val Gly
 1               5                  10                  15      
Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile
            20                  25                  30          
Phe Trp
        


<210> 6

<400> 6
 000

<210> 7

<400> 7
 000

<210> 8
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> T2A ribosomal skip element

<400> 8
Tyr Gly Pro Pro Cys Pro Pro Cys Pro
 1               5                  


<210> 9
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> tEGFR

<400> 9
Tyr Gly Pro Pro Cys Pro Pro Cys Pro
 1               5                  


<210> 10
<211> 1152
<212> DNA
<213> Artificial Sequence

<220> 
<223> T2A domain

<400> 10
ccaaggctcg agggcggcgg agagggcaga ggaagtcttc taacatgcgg tgacgtggag 60
gagaatcccg gccctaggat gcttctcctg gtgacaagcc ttctgctctg tgagttacca 120
cacccagcat tcctcctgat cccacgcaaa gtgtgtaacg gaataggtat tggtgaattt 180
aaagactcac tctccataaa tgctacgaat attaaacact tcaaaaactg cacctccatc 240
agtggcgatc tccacatcct gccggtggca tttaggggtg actccttcac acatactcct 300
cctctggatc cacaggaact ggatattctg aaaaccgtaa aggaaatcac agggtttttg 360
ctgattcagg cttggcctga aaacaggacg gacctccatg cctttgagaa cctagaaatc 420
atacgcggca ggaccaagca acatggtcag ttttctcttg cagtcgtcag cctgaacata 480
acatccttgg gattacgctc cctcaaggag ataagtgatg gagatgtgat aatttcagga 540
aacaaaaatt tgtgctatgc aaatacaata aactggaaaa aactgtttgg gacctccggt 600
cagaaaacca aaattataag caacagaggt gaaaacagct gcaaggccac aggccaggtc 660
tgccatgcct tgtgctcccc cgagggctgc tggggcccgg agcccaggga ctgcgtctct 720
tgccggaatg tcagccgagg cagggaatgc gtggacaagt gcaaccttct ggagggtgag 780
ccaagggagt ttgtggagaa ctctgagtgc atacagtgcc acccagagtg cctgcctcag 840
gccatgaaca tcacctgcac aggacgggga ccagacaact gtatccagtg tgcccactac 900
attgacggcc cccactgcgt caagacctgc ccggcaggag tcatgggaga aaacaacacc 960
ctggtctgga agtacgcaga cgccggccat gtgtgccacc tgtgccatcc aaactgcacc 1020
tacggatgca ctgggccagg tcttgaaggc tgtccaacga atgggcctaa gatcccgtcc 1080
atcgccactg ggatggtggg ggccctcctc ttgctgctgg tggtggccct ggggatcggc 1140
ctcttcatgt ga                                                     1152

<210> 11
<211> 383
<212> PRT
<213> Artificial Sequence

<220> 
<223> T2A domain

<400> 11
Pro Arg Leu Glu Gly Gly Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys
 1               5                  10                  15      
Gly Asp Val Glu Glu Asn Pro Gly Pro Arg Met Leu Leu Leu Val Thr
            20                  25                  30          
Ser Leu Leu Leu Cys Glu Leu Pro His Pro Ala Phe Leu Leu Ile Pro
        35                  40                  45              
Arg Lys Val Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu
    50                  55                  60                  
Ser Ile Asn Ala Thr Asn Ile Lys His Phe Lys Asn Cys Thr Ser Ile
65                  70                  75                  80  
Ser Gly Asp Leu His Ile Leu Pro Val Ala Phe Arg Gly Asp Ser Phe
                85                  90                  95      
Thr His Thr Pro Pro Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr
            100                 105                 110         
Val Lys Glu Ile Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn
        115                 120                 125             
Arg Thr Asp Leu His Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg
    130                 135                 140                 
Thr Lys Gln His Gly Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile
145                 150                 155                 160 
Thr Ser Leu Gly Leu Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp Val
                165                 170                 175     
Ile Ile Ser Gly Asn Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp
            180                 185                 190         
Lys Lys Leu Phe Gly Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn
        195                 200                 205             
Arg Gly Glu Asn Ser Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu
    210                 215                 220                 
Cys Ser Pro Glu Gly Cys Trp Gly Pro Glu Pro Arg Asp Cys Val Ser
225                 230                 235                 240 
Cys Arg Asn Val Ser Arg Gly Arg Glu Cys Val Asp Lys Cys Asn Leu
                245                 250                 255     
Leu Glu Gly Glu Pro Arg Glu Phe Val Glu Asn Ser Glu Cys Ile Gln
            260                 265                 270         
Cys His Pro Glu Cys Leu Pro Gln Ala Met Asn Ile Thr Cys Thr Gly
        275                 280                 285             
Arg Gly Pro Asp Asn Cys Ile Gln Cys Ala His Tyr Ile Asp Gly Pro
    290                 295                 300                 
His Cys Val Lys Thr Cys Pro Ala Gly Val Met Gly Glu Asn Asn Thr
305                 310                 315                 320 
Leu Val Trp Lys Tyr Ala Asp Ala Gly His Val Cys His Leu Cys His
                325                 330                 335     
Pro Asn Cys Thr Tyr Gly Cys Thr Gly Pro Gly Leu Glu Gly Cys Pro
            340                 345                 350         
Thr Asn Gly Pro Lys Ile Pro Ser Ile Ala Thr Gly Met Val Gly Ala
        355                 360                 365             
Leu Leu Leu Leu Leu Val Val Ala Leu Gly Ile Gly Leu Phe Met
    370                 375                 380             


<210> 12

<400> 12
 000

<210> 13
<211> 326
<212> PRT
<213> Artificial Sequence

<220> 
<223> Exemplary IgG4-Fc

<400> 13
Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg
 1               5                  10                  15      
Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr
            20                  25                  30          
Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser
        35                  40                  45              
Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser
    50                  55                  60                  
Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr
65                  70                  75                  80  
Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys
                85                  90                  95      
Arg Val Glu Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro Glu
            100                 105                 110         
Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp
        115                 120                 125             
Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
    130                 135                 140                 
Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly
145                 150                 155                 160 
Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn
                165                 170                 175     
Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp
            180                 185                 190         
Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro
        195                 200                 205             
Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
    210                 215                 220                 
Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn
225                 230                 235                 240 
Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile
                245                 250                 255     
Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr
            260                 265                 270         
Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg
        275                 280                 285             
Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys
    290                 295                 300                 
Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
305                 310                 315                 320 
Ser Leu Ser Leu Gly Lys
                325     


<210> 14
<211> 220
<212> PRT
<213> Artificial Sequence

<220> 
<223> Modified IgG4 hinge region

<400> 14
Met Leu Arg Leu Leu Leu Ala Leu Asn Leu Phe Pro Ser Ile Gln Val
 1               5                  10                  15      
Thr Gly Asn Lys Ile Leu Val Lys Gln Ser Pro Met Leu Val Ala Tyr
            20                  25                  30          
Asp Asn Ala Val Asn Leu Ser Cys Lys Tyr Ser Tyr Asn Leu Phe Ser
        35                  40                  45              
Arg Glu Phe Arg Ala Ser Leu His Lys Gly Leu Asp Ser Ala Val Glu
    50                  55                  60                  
Val Cys Val Val Tyr Gly Asn Tyr Ser Gln Gln Leu Gln Val Tyr Ser
65                  70                  75                  80  
Lys Thr Gly Phe Asn Cys Asp Gly Lys Leu Gly Asn Glu Ser Val Thr
                85                  90                  95      
Phe Tyr Leu Gln Asn Leu Tyr Val Asn Gln Thr Asp Ile Tyr Phe Cys
            100                 105                 110         
Lys Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser
        115                 120                 125             
Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro
    130                 135                 140                 
Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly
145                 150                 155                 160 
Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile
                165                 170                 175     
Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met
            180                 185                 190         
Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro
        195                 200                 205             
Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser
    210                 215                 220 


<210> 15
<211> 255
<212> PRT
<213> Artificial Sequence

<220> 
<223> Cytoplasmic domain of human 4-1BB

<400> 15
Met Gly Asn Ser Cys Tyr Asn Ile Val Ala Thr Leu Leu Leu Val Leu
 1               5                  10                  15      
Asn Phe Glu Arg Thr Arg Ser Leu Gln Asp Pro Cys Ser Asn Cys Pro
            20                  25                  30          
Ala Gly Thr Phe Cys Asp Asn Asn Arg Asn Gln Ile Cys Ser Pro Cys
        35                  40                  45              
Pro Pro Asn Ser Phe Ser Ser Ala Gly Gly Gln Arg Thr Cys Asp Ile
    50                  55                  60                  
Cys Arg Gln Cys Lys Gly Val Phe Arg Thr Arg Lys Glu Cys Ser Ser
65                  70                  75                  80  
Thr Ser Asn Ala Glu Cys Asp Cys Thr Pro Gly Phe His Cys Leu Gly
                85                  90                  95      
Ala Gly Cys Ser Met Cys Glu Gln Asp Cys Lys Gln Gly Gln Glu Leu
            100                 105                 110         
Thr Lys Lys Gly Cys Lys Asp Cys Cys Phe Gly Thr Phe Asn Asp Gln
        115                 120                 125             
Lys Arg Gly Ile Cys Arg Pro Trp Thr Asn Cys Ser Leu Asp Gly Lys
    130                 135                 140                 
Ser Val Leu Val Asn Gly Thr Lys Glu Arg Asp Val Val Cys Gly Pro
145                 150                 155                 160 
Ser Pro Ala Asp Leu Ser Pro Gly Ala Ser Ser Val Thr Pro Pro Ala
                165                 170                 175     
Pro Ala Arg Glu Pro Gly His Ser Pro Gln Ile Ile Ser Phe Phe Leu
            180                 185                 190         
Ala Leu Thr Ser Thr Ala Leu Leu Phe Leu Leu Phe Phe Leu Thr Leu
        195                 200                 205             
Arg Phe Ser Val Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe
    210                 215                 220                 
Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly
225                 230                 235                 240 
Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu
                245                 250                 255 


<210> 16
<211> 164
<212> PRT
<213> Artificial Sequence

<220> 
<223> Cytoplasmic domain of isoform 3 of human CD3 zeta

<400> 16
Met Lys Trp Lys Ala Leu Phe Thr Ala Ala Ile Leu Gln Ala Gln Leu
 1               5                  10                  15      
Pro Ile Thr Glu Ala Gln Ser Phe Gly Leu Leu Asp Pro Lys Leu Cys
            20                  25                  30          
Tyr Leu Leu Asp Gly Ile Leu Phe Ile Tyr Gly Val Ile Leu Thr Ala
        35                  40                  45              
Leu Phe Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr
    50                  55                  60                  
Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg
65                  70                  75                  80  
Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met
                85                  90                  95      
Gly Gly Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn
            100                 105                 110         
Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met
        115                 120                 125             
Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly
    130                 135                 140                 
Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala
145                 150                 155                 160 
Leu Pro Pro Arg
                


<210> 17
<211> 15
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human IgG1 hinge region

<400> 17
Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro
 1               5                  10                  15  


<210> 18
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human IgG2 hinge region

<400> 18
Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro
 1               5                  10          


<210> 19
<211> 61
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human IgG3 hinge region

<400> 19
Glu Leu Lys Thr Pro Leu Gly Asp Thr His Thr Cys Pro Arg Cys Pro
 1               5                  10                  15      
Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro Glu
            20                  25                  30          
Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro Glu Pro
        35                  40                  45              
Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro
    50                  55                  60      


<210> 20
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> Human IgG4 hinge region

<400> 20
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro
 1               5                  10          


<210> 21
<211> 12
<212> PRT
<213> Artificial Sequence

<220> 
<223> Modified Human IgG4 hinge region

<400> 21
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro
 1               5                  10          


<210> 22

<400> 22
 000

<210> 23

<400> 23
 000

<210> 24

<400> 24
 000

<210> 25

<400> 25
 000

<210> 26

<400> 26
 000

<210> 27

<400> 27
 000

<210> 28

<400> 28
 000

<210> 29

<400> 29
 000

<210> 30

<400> 30
 000

<210> 31

<400> 31
 000

<210> 32

<400> 32
 000

<210> 33

<400> 33
 000

<210> 34

<400> 34
 000

<210> 35

<400> 35
 000

<210> 36

<400> 36
 000

<210> 37
<211> 348
<212> DNA
<213> Artificial Sequence

<220> 
<223> Medium spacer IgG4 hinge-CH3

<400> 37
tacggaccgc cctgcccccc ttgccctggc cagcctcgcg agccccaggt gtacaccctg 60
cctccctccc aggaagagat gaccaagaac caggtgtccc tgacctgcct ggtgaagggc 120
ttctacccca gcgacatcgc cgtggagtgg gagagcaacg gccagcctga gaacaactac 180
aagaccaccc ctcccgtgct ggacagcgac ggcagcttct tcctgtacag ccggctgacc 240
gtggacaaga gccggtggca ggaaggcaac gtctttagct gcagcgtgat gcacgaggcc 300
ctgcacaacc actacaccca gaagagcctg agcctgtccc tgggcaag              348

<210> 38

<400> 38
 000

<210> 39

<400> 39
 000

<210> 40

<400> 40
 000

<210> 41

<400> 41
 000

<210> 42

<400> 42
 000

<210> 43

<400> 43
 000

<210> 44

<400> 44
 000

<210> 45

<400> 45
 000

<210> 46

<400> 46
 000

<210> 47

<400> 47
 000

<210> 48

<400> 48
 000

<210> 49
<211> 5
<212> PRT
<213> Artificial Sequence

<220> 
<223> IgG4 hinge

<400> 49
Glu Ser Lys Tyr Gly
 1               5  


<210> 50

<400> 50
 000

<210> 51
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Modified Human IgG4 hinge region

<400> 51
Tyr Gly Pro Pro Cys Pro Pro Cys Pro
 1               5                  


<210> 52
<211> 10
<212> PRT
<213> Artificial Sequence

<220> 
<223> Modified Human IgG4 hinge region

<400> 52
Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro
 1               5                  10  


<210> 53
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> Modified Human IgG4 hinge region

<400> 53
Glu Val Val Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro
 1               5                  10              


<210> 54
<211> 20048
<212> DNA
<213> Artificial Sequence

<220> 
<223> Ce7scFv-IgG4hinge-CH2-CH3-CD28tm/4-1BB-zeta-T2A-EG
      FRt-epHIV7

<400> 54
gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 60
tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc caatctggtc tagactcgga 120
ccctcgagag accgattgat cccttgggtg acgaattcgg agttatttcg aacggaactc 180
acgaagttca tcacacacgg cgtctgttgt gtgactctgg taactagaga tccctcagac 240
ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa 300
gcagacaaca cactgagacc attgatctct agggagtctg ggaaaatcag tcacaccttt 360
tagagatcgt caccgcgggc ttgtccctga actttcgctt agggaaacca gaggagctct 420
ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg 480
tgagtacgcc aaaaattttg tccctttggt ctcctcgaga gagctgcgtc ctgagccgaa 540
cgacttcgcg cgtgccgttc tccgctcccc gccgctgacc actcatgcgg tttttaaaac 600
actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 660
attagatcga tgggaaaaaa ttcggttaag gccaggggga tgatcgcctc cgatcttcct 720
ctctctaccc acgctctcgc agtcataatt cgccccctct taatctagct accctttttt 780
aagccaattc cggtccccct aagaaaaaat ataaattaaa acatatagta tgggcaagca 840
gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac 900
ttctttttta tatttaattt tgtatatcat acccgttcgt ccctcgatct tgctaagcgt 960
caattaggac cggacaatct ttgtagtctt ccgacatctg aaatactggg acagctacaa 1020
ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc 1080
tattgtgtgc atcaaaggat tttatgaccc tgtcgatgtt ggtagggaag tctgtcctag 1140
tcttcttgaa tctagtaata tattatgtca tcgttgggag ataacacacg tagtttccta 1200
agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa 1260
gaaaaaagca cagcaagcag cagctgacac aggacacagc tctctatttt ctgtggttcc 1320
ttcgaaatct gttctatctc cttctcgttt tgttttcatt cttttttcgt gtcgttcgtc 1380
gtcgactgtg tcctgtgtcg aatcaggtca gccaaaatta ccctatagtg cagaacatcc 1440
aggggcaaat ggtacatcag gccatatcac ctagaacttt aaatgcatgg gtaaaagtag 1500
ttagtccagt cggttttaat gggatatcac gtcttgtagg tccccgttta ccatgtagtc 1560
cggtatagtg gatcttgaaa tttacgtacc cattttcatc tagaagagaa ggctttcagc 1620
ccagaagtga tacccatgtt ttcagcatta tcagaaggag ccaccccaca agatttaaac 1680
accatgctaa acacagtggg atcttctctt ccgaaagtcg ggtcttcact atgggtacaa 1740
aagtcgtaat agtcttcctc ggtggggtgt tctaaatttg tggtacgatt tgtgtcaccc 1800
gggacatcaa gcagccatgc aaatgttaaa agagaccatc aatgaggaag ctgcaggcaa 1860
agagaagagt ggtgcagaga gaaaaaagag cagtgggaat ccctgtagtt cgtcggtacg 1920
tttacaattt tctctggtag ttactccttc gacgtccgtt tctcttctca ccacgtctct 1980
cttttttctc gtcaccctta aggagctttg ttccttgggt tcttgggagc agcaggaagc 2040
actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata 2100
tcctcgaaac aaggaaccca agaaccctcg tcgtccttcg tgatacccgc gtcgcagtta 2160
ctgcgactgc catgtccggt ctgttaataa cagaccatat gtgcagcagc agaacaattt 2220
gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 2280
gctccaggca agaatcctgg cacgtcgtcg tcttgttaaa cgactcccga taactccgcg 2340
ttgtcgtaga caacgttgag tgtcagaccc cgtagttcgt cgaggtccgt tcttaggacc 2400
ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac 2460
tcatttgcac cactgctgtg ccttggatct acaaatggca gacacctttc tatggatttc 2520
ctagttgtcg aggaccccta aaccccaacg agaccttttg agtaaacgtg gtgacgacac 2580
ggaacctaga tgtttaccgt gtattcatcc acaattttaa aagaaaaggg gggattgggg 2640
ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat 2700
cataagtagg tgttaaaatt ttcttttccc ccctaacccc ccatgtcacg tcccctttct 2760
tatcatctgt attatcgttg tctgtatgtt tgatttctta tacaaaaaca aattacaaaa 2820
attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttgggg atcaattgca 2880
tgaagaatct gcttagggtt atgtttttgt ttaatgtttt taagttttaa aagcccaaat 2940
aatgtccctg tcgtctctag gtcaaacccc tagttaacgt acttcttaga cgaatcccaa 3000
aggcgttttg cgctgcttcg cgaggatctg cgatcgctcc ggtgcccgtc agtgggcaga 3060
gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg tccgcaaaac gcgacgaagc 3120
gctcctagac gctagcgagg ccacgggcag tcacccgtct cgcgtgtagc gggtgtcagg 3180
ggctcttcaa cccccctccc gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg 3240
taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac 3300
cagccgttaa cttggccacg gatctcttcc accgcgcccc atttgaccct ttcactacag 3360
cacatgaccg aggcggaaaa agggctccca ccccctcttg cgtatataag tgcagtagtc 3420
gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 3480
gctcgcatct ctccttcacg gcatatattc acgtcatcag cggcacttgc aagaaaaagc 3540
gttgcccaaa cggcggtctt gtgtcgactt cgaagctccc cgagcgtaga gaggaagtgc 3600
cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc 3660
cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta gcgggcggcg ggatggactc 3720
cggcggtagg tgcggccaac tcagcgcaag acggcggagg gcggacacca cggaggactt 3780
gacgcaggcg gcagatccat agtttaaagc tcaggtcgag accgggcctt tgtccggcgc 3840
tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc 3900
tcaaatttcg agtccagctc tggcccggaa acaggccgcg agggaacctc ggatggatct 3960
gagtcggccg agaggtgcga aacggactgg gacgaacgag aactctacgt ctttgtttcg 4020
ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacggc tagcgccgcc 4080
accatgctgc tgctggtgac ttgagatgca gaaacaaagc aaaagacaag acgcggcaat 4140
gtctaggttc gacactggcc gcggatgccg atcgcggcgg tggtacgacg acgaccactg 4200
cagcctgctg ctgtgcgagc tgccccaccc cgcctttctg ctgatccccc aggtgcagct 4260
gcagcagcct ggcgccgagc tggtgaagcc aggcgccagc gtcggacgac gacacgctcg 4320
acggggtggg gcggaaagac gactaggggg tccacgtcga cgtcgtcgga ccgcggctcg 4380
accacttcgg tccgcggtcg gtgaagctgt cctgcaaggc cagcggctac accttcaccg 4440
gctactggat gcactgggtg aagcagagac ccggccacgg cctggaatgg atcggcgaga 4500
cacttcgaca ggacgttccg gtcgccgatg tggaagtggc cgatgaccta cgtgacccac 4560
ttcgtctctg ggccggtgcc ggaccttacc tagccgctct tcaaccccag caacggccgg 4620
accaactaca acgagcggtt caagagcaag gccaccctga ccgtggacaa gagcagcacc 4680
accgccttca tgcagctgtc agttggggtc gttgccggcc tggttgatgt tgctcgccaa 4740
gttctcgttc cggtgggact ggcacctgtt ctcgtcgtgg tggcggaagt acgtcgacag 4800
cggcctgacc agcgaggaca gcgccgtgta cttctgcgcc agggactact acggcaccag 4860
ctacaacttc gactactggg gccagggcac cacactgacc gccggactgg tcgctcctgt 4920
cgcggcacat gaagacgcgg tccctgatga tgccgtggtc gatgttgaag ctgatgaccc 4980
cggtcccgtg gtgtgactgg gtgagcagcg gcggaggggg ctctggcggc ggaggatctg 5040
ggggaggggg cagcgacatc cagatgaccc agagcagcag cagcttcagc gtgagcctgg 5100
cactcgtcgc cgcctccccc gagaccgccg cctcctagac cccctccccc gtcgctgtag 5160
gtctactggg tctcgtcgtc gtcgaagtcg cactcggacc gcgaccgggt gaccatcacc 5220
tgtaaggcca acgaggacat caacaaccgg ctggcctggt atcagcagac ccccggcaac 5280
agccccaggc tgctgatcag cgctggccca ctggtagtgg acattccggt tgctcctgta 5340
gttgttggcc gaccggacca tagtcgtctg ggggccgttg tcggggtccg acgactagtc 5400
cggcgccacc aacctggtga ccggcgtgcc cagccggttt agcggcagcg gctccggcaa 5460
ggactacacc ctgaccatca caagcctgca ggccgaggac gccgcggtgg ttggaccact 5520
ggccgcacgg gtcggccaaa tcgccgtcgc cgaggccgtt cctgatgtgg gactggtagt 5580
gttcggacgt ccggctcctg ttcgccacct actactgcca gcagtactgg tccaccccct 5640
tcaccttcgg cagcggcacc gagctggaaa tcaaagagag caagtacgga ccgccctgcc 5700
aagcggtgga tgatgacggt cgtcatgacc aggtggggga agtggaagcc gtcgccgtgg 5760
ctcgaccttt agtttctctc gttcatgcct ggcgggacgg ccccttgccc tgcccccgag 5820
ttcctgggcg gacccagcgt gttcctgttc ccccccaagc ccaaggacac cctgatgatc 5880
agccggaccc ccgaggtgac ggggaacggg acgggggctc aaggacccgc ctgggtcgca 5940
caaggacaag ggggggttcg ggttcctgtg ggactactag tcggcctggg ggctccactg 6000
ctgcgtggtg gtggacgtga gccaggaaga tcccgaggtc cagttcaatt ggtacgtgga 6060
cggcgtggaa gtgcacaacg ccaagaccaa gcccagagag gacgcaccac cacctgcact 6120
cggtccttct agggctccag gtcaagttaa ccatgcacct gccgcacctt cacgtgttgc 6180
ggttctggtt cgggtctctc gaacagttca acagcaccta ccgggtggtg tctgtgctga 6240
ccgtgctgca ccaggactgg ctgaacggca aagaatacaa gtgcaaggtg tccaacaagg 6300
cttgtcaagt tgtcgtggat ggcccaccac agacacgact ggcacgacgt ggtcctgacc 6360
gacttgccgt ttcttatgtt cacgttccac aggttgttcc gcctgcccag cagcatcgaa 6420
aagaccatca gcaaggccaa gggccagcct cgcgagcccc aggtgtacac cctgcctccc 6480
tcccaggaag agatgaccaa cggacgggtc gtcgtagctt ttctggtagt cgttccggtt 6540
cccggtcgga gcgctcgggg tccacatgtg ggacggaggg agggtccttc tctactggtt 6600
gaaccaggtg tccctgacct gcctggtgaa gggcttctac cccagcgaca tcgccgtgga 6660
gtgggagagc aacggccagc ctgagaacaa ctacaagacc cttggtccac agggactgga 6720
cggaccactt cccgaagatg gggtcgctgt agcggcacct caccctctcg ttgccggtcg 6780
gactcttgtt gatgttctgg acccctcccg tgctggacag cgacggcagc ttcttcctgt 6840
acagccggct gaccgtggac aagagccggt ggcaggaagg caacgtcttt agctgcagcg 6900
tggggagggc acgacctgtc gctgccgtcg aagaaggaca tgtcggccga ctggcacctg 6960
ttctcggcca ccgtccttcc gttgcagaaa tcgacgtcgc tgatgcacga ggccctgcac 7020
aaccactaca cccagaagag cctgagcctg tccctgggca agatgttctg ggtgctggtg 7080
gtggtgggcg gggtgctggc actacgtgct ccgggacgtg ttggtgatgt gggtcttctc 7140
ggactcggac agggacccgt tctacaagac ccacgaccac caccacccgc cccacgaccg 7200
ctgctacagc ctgctggtga cagtggcctt catcatcttt tgggtgaaac ggggcagaaa 7260
gaaactcctg tatatattca aacaaccatt tatgagacca gacgatgtcg gacgaccact 7320
gtcaccggaa gtagtagaaa acccactttg ccccgtcttt ctttgaggac atatataagt 7380
ttgttggtaa atactctggt gtacaaacta ctcaagagga agatggctgt agctgccgat 7440
ttccagaaga agaagaagga ggatgtgaac tgcgggtgaa gttcagcaga agcgccgacg 7500
catgtttgat gagttctcct tctaccgaca tcgacggcta aaggtcttct tcttcttcct 7560
cctacacttg acgcccactt caagtcgtct tcgcggctgc cccctgccta ccagcagggc 7620
cagaatcagc tgtacaacga gctgaacctg ggcagaaggg aagagtacga cgtcctggat 7680
aagcggagag gccgggaccc ggggacggat ggtcgtcccg gtcttagtcg acatgttgct 7740
cgacttggac ccgtcttccc ttctcatgct gcaggaccta ttcgcctctc cggccctggg 7800
tgagatgggc ggcaagcctc ggcggaagaa cccccaggaa ggcctgtata acgaactgca 7860
gaaagacaag atggccgagg cctacagcga gatcggcatg actctacccg ccgttcggag 7920
ccgccttctt gggggtcctt ccggacatat tgcttgacgt ctttctgttc taccggctcc 7980
ggatgtcgct ctagccgtac aagggcgagc ggaggcgggg caagggccac gacggcctgt 8040
atcagggcct gtccaccgcc accaaggata cctacgacgc cctgcacatg caggccctgc 8100
ttcccgctcg cctccgcccc gttcccggtg ctgccggaca tagtcccgga caggtggcgg 8160
tggttcctat ggatgctgcg ggacgtgtac gtccgggacg ccccaaggct cgagggcggc 8220
ggagagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctagg 8280
atgcttctcc tggtgacaag ggggttccga gctcccgccg cctctcccgt ctccttcaga 8340
agattgtacg ccactgcacc tcctcttagg gccgggatcc tacgaagagg accactgttc 8400
ccttctgctc tgtgagttac cacacccagc attcctcctg atcccacgca aagtgtgtaa 8460
cggaataggt attggtgaat ttaaagactc actctccata ggaagacgag acactcaatg 8520
gtgtgggtcg taaggaggac tagggtgcgt ttcacacatt gccttatcca taaccactta 8580
aatttctgag tgagaggtat aatgctacga atattaaaca cttcaaaaac tgcacctcca 8640
tcagtggcga tctccacatc ctgccggtgg catttagggg tgactccttc acacatactc 8700
ttacgatgct tataatttgt gaagtttttg acgtggaggt agtcaccgct agaggtgtag 8760
gacggccacc gtaaatcccc actgaggaag tgtgtatgag ctcctctgga tccacaggaa 8820
ctggatattc tgaaaaccgt aaaggaaatc acagggtttt tgctgattca ggcttggcct 8880
gaaaacagga cggacctcca gaggagacct aggtgtcctt gacctataag acttttggca 8940
tttcctttag tgtcccaaaa acgactaagt ccgaaccgga cttttgtcct gcctggaggt 9000
tgcctttgag aacctagaaa tcatacgcgg caggaccaag caacatggtc agttttctct 9060
tgcagtcgtc agcctgaaca taacatcctt gggattacgc acggaaactc ttggatcttt 9120
agtatgcgcc gtcctggttc gttgtaccag tcaaaagaga acgtcagcag tcggacttgt 9180
attgtaggaa ccctaatgcg tccctcaagg agataagtga tggagatgtg ataatttcag 9240
gaaacaaaaa tttgtgctat gcaaatacaa taaactggaa aaaactgttt gggacctccg 9300
agggagttcc tctattcact acctctacac tattaaagtc ctttgttttt aaacacgata 9360
cgtttatgtt atttgacctt ttttgacaaa ccctggaggc gtcagaaaac caaaattata 9420
agcaacagag gtgaaaacag ctgcaaggcc acaggccagg tctgccatgc cttgtgctcc 9480
cccgagggct gctggggccc cagtcttttg gttttaatat tcgttgtctc cacttttgtc 9540
gacgttccgg tgtccggtcc agacggtacg gaacacgagg gggctcccga cgaccccggg 9600
ggagcccagg gactgcgtct cttgccggaa tgtcagccga ggcagggaat gcgtggacaa 9660
gtgcaacctt ctggagggtg agccaaggga gtttgtggag cctcgggtcc ctgacgcaga 9720
gaacggcctt acagtcggct ccgtccctta cgcacctgtt cacgttggaa gacctcccac 9780
tcggttccct caaacacctc aactctgagt gcatacagtg ccacccagag tgcctgcctc 9840
aggccatgaa catcacctgc acaggacggg gaccagacaa ctgtatccag tgtgcccact 9900
ttgagactca cgtatgtcac ggtgggtctc acggacggag tccggtactt gtagtggacg 9960
tgtcctgccc ctggtctgtt gacataggtc acacgggtga acattgacgg cccccactgc 10020
gtcaagacct gcccggcagg agtcatggga gaaaacaaca ccctggtctg gaagtacgca 10080
gacgccggcc atgtgtgcca tgtaactgcc gggggtgacg cagttctgga cgggccgtcc 10140
tcagtaccct cttttgttgt gggaccagac cttcatgcgt ctgcggccgg tacacacggt 10200
cctgtgccat ccaaactgca cctacggatg cactgggcca ggtcttgaag gctgtccaac 10260
gaatgggcct aagatcccgt ccatcgccac tgggatggtg ggacacggta ggtttgacgt 10320
ggatgcctac gtgacccggt ccagaacttc cgacaggttg cttacccgga ttctagggca 10380
ggtagcggtg accctaccac ggggccctcc tcttgctgct ggtggtggcc ctggggatcg 10440
gcctcttcat gtgagcggcc gctctagacc cgggctgcag gaattcgata tcaagcttat 10500
ccccgggagg agaacgacga ccaccaccgg gacccctagc cggagaagta cactcgccgg 10560
cgagatctgg gcccgacgtc cttaagctat agttcgaata cgataatcaa cctctggatt 10620
acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 10680
gatacgctgc tttaatgcct gctattagtt ggagacctaa tgttttaaac actttctaac 10740
tgaccataag aattgataca acgaggaaaa tgcgatacac ctatgcgacg aaattacgga 10800
ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg 10860
ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacatagtac gataacgaag 10920
ggcataccga aagtaaaaga ggaggaacat atttaggacc aacgacagag aaatactcct 10980
caacaccggg caacagtccg aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc 11040
cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct 11100
ttgcaccgca ccacacgtga cacaaacgac tgcgttgggg gtgaccaacc ccgtaacggt 11160
ggtggacagt cgaggaaagg ccctgaaagc gaaaggggga ccctattgcc acggcggaac 11220
tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 11280
ccgtggtgtt gtcggggaaa gggataacgg tgccgccttg agtagcggcg gacggaacgg 11340
gcgacgacct gtccccgagc cgacaacccg tgactgttaa ggcaccacaa cagccccttt 11400
tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc 11460
ttctgctacg tcccttcggc cctcaatcca gcggaccttc agtagcagga aaggaaccga 11520
cgagcggaca caacggtgga cctaagacgc gccctgcagg aagacgatgc agggaagccg 11580
ggagttaggt cgcctggaag cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg 11640
tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata 11700
gaagggcgcc ggacgacggc cgagacgccg gagaaggcgc agaagcggaa gcgggagtct 11760
gctcagccta gagggaaacc cggcggaggg gcgtagctat ccgtcgacta gccgtacctt 11820
taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg 11880
gactggaagg gctaattcac ggcagctgat cggcatggaa attctggtta ctgaatgttc 11940
cgtcgacatc tagaatcggt gaaaaatttt cttttccccc ctgaccttcc cgattaagtg 12000
tcccaaagaa gacaagatct gctttttgcc tgtactgggt ctctctggtt agaccagatc 12060
tgagcctggg agctctctgg ctaactaggg aacccactgc agggtttctt ctgttctaga 12120
cgaaaaacgg acatgaccca gagagaccaa tctggtctag actcggaccc tcgagagacc 12180
gattgatccc ttgggtgacg ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt 12240
gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 12300
aattcggagt tatttcgaac ggaactcacg aagttcatca cacacgggca gacaacacac 12360
tgagaccatt gatctctagg gagtctggga aaatcagtca gtggaaaatc tctagcagaa 12420
ttcgatatca agcttatcga taccgtcgac ctcgaggggg ggcccggtac ccaattcgcc 12480
ctatagtgag tcgtattaca caccttttag agatcgtctt aagctatagt tcgaatagct 12540
atggcagctg gagctccccc ccgggccatg ggttaagcgg gatatcactc agcataatgt 12600
attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta 12660
atcgccttgc agcacatccc cctttcgcca gctggcgtaa taagtgaccg gcagcaaaat 12720
gttgcagcac tgaccctttt gggaccgcaa tgggttgaat tagcggaacg tcgtgtaggg 12780
ggaaagcggt cgaccgcatt tagcgaagag gcccgcaccg atcgcccttc ccaacagttg 12840
cgcagcctga atggcgaatg gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa 12900
atcgcttctc cgggcgtggc tagcgggaag ggttgtcaac gcgtcggact taccgcttac 12960
ctttaacatt cgcaattata aaacaatttt aagcgcaatt atttttgtta aatcagctca 13020
ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag 13080
atagggttga gtgttgttcc taaaaacaat ttagtcgagt aaaaaattgg ttatccggct 13140
ttagccgttt tagggaatat ttagttttct tatctggctc tatcccaact cacaacaagg 13200
agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac 13260
cgtctatcag ggcgatggcc cactacgtga accatcaccc tcaaaccttg ttctcaggtg 13320
ataatttctt gcacctgagg ttgcagtttc ccgctttttg gcagatagtc ccgctaccgg 13380
gtgatgcact tggtagtggg taatcaagtt ttttggggtc gaggtgccgt aaagcactaa 13440
atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg 13500
attagttcaa aaaaccccag ctccacggca tttcgtgatt tagccttggg atttccctcg 13560
ggggctaaat ctcgaactgc ccctttcggc cgcttgcacc cgagaaagga agggaagaaa 13620
gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc 13680
acacccgccg cgcttaatgc gctctttcct tcccttcttt cgctttcctc gcccgcgatc 13740
ccgcgaccgt tcacatcgcc agtgcgacgc gcattggtgg tgtgggcggc gcgaattacg 13800
gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 13860
tttatttttc taaatacatt caaatatgta tccgctcatg cggcgatgtc ccgcgcagtc 13920
caccgtgaaa agccccttta cacgcgcctt ggggataaac aaataaaaag atttatgtaa 13980
gtttatacat aggcgagtac agacaataac cctgataaat gcttcaataa tattgaaaaa 14040
ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 14100
tctgttattg ggactattta cgaagttatt ataacttttt ccttctcata ctcataagtt 14160
gtaaaggcac agcgggaata agggaaaaaa cgccgtaaaa gccttcctgt ttttgctcac 14220
ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 14280
atcgaactgg atctcaacag cggaaggaca aaaacgagtg ggtctttgcg accactttca 14340
ttttctacga cttctagtca acccacgtgc tcacccaatg tagcttgacc tagagttgtc 14400
cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 14460
agttctgcta tgtggcgcgg tattatcccg tattgacgcc gccattctag gaactctcaa 14520
aagcggggct tcttgcaaaa ggttactact cgtgaaaatt tcaagacgat acaccgcgcc 14580
ataatagggc ataactgcgg gggcaagagc aactcggtcg ccgcatacac tattctcaga 14640
atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 14700
cccgttctcg ttgagccagc ggcgtatgtg ataagagtct tactgaacca actcatgagt 14760
ggtcagtgtc ttttcgtaga atgcctaccg tactgtcatt gagaattatg cagtgctgcc 14820
ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 14880
gagctaaccg cttttttgca ctcttaatac gtcacgacgg tattggtact cactattgtg 14940
acgccggttg aatgaagact gttgctagcc tcctggcttc ctcgattggc gaaaaaacgt 15000
caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 15060
accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gttgtacccc ctagtacatt 15120
gagcggaact agcaaccctt ggcctcgact tacttcggta tggtttgctg ctcgcactgt 15180
ggtgctacgg acatcgttac gcaacaacgt tgcgcaaact attaactggc gaactactta 15240
ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 15300
cgttgttgca acgcgtttga taattgaccg cttgatgaat gagatcgaag ggccgttgtt 15360
aattatctga cctacctccg cctatttcaa cgtcctggtg ttctgcgctc ggcccttccg 15420
gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 15480
gcagcactgg ggccagatgg aagacgcgag ccgggaaggc cgaccgacca aataacgact 15540
atttagacct cggccactcg cacccagagc gccatagtaa cgtcgtgacc ccggtctacc 15600
taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 15660
aaatagacag atcgctgaga taggtgcctc actgattaag attcgggagg gcatagcatc 15720
aatagatgtg ctgcccctca gtccgttgat acctacttgc tttatctgtc tagcgactct 15780
atccacggag tgactaattc cattggtaac tgtcagacca agtttactca tatatacttt 15840
agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 15900
gtaaccattg acagtctggt tcaaatgagt atatatgaaa tctaactaaa ttttgaagta 15960
aaaattaaat tttcctagat ccacttctag gaaaaactat atctcatgac caaaatccct 16020
taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 16080
tgagatcctt tttttctgcg tagagtactg gttttaggga attgcactca aaagcaaggt 16140
gactcgcagt ctggggcatc ttttctagtt tcctagaaga actctaggaa aaaaagacgc 16200
cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 16260
tcaagagcta ccaactcttt ttccgaaggt aactggcttc gcattagacg acgaacgttt 16320
gtttttttgg tggcgatggt cgccaccaaa caaacggcct agttctcgat ggttgagaaa 16380
aaggcttcca ttgaccgaag agcagagcgc agataccaaa tactgttctt ctagtgtagc 16440
cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 16500
tcgtctcgcg tctatggttt atgacaagaa gatcacatcg gcatcaatcc ggtggtgaag 16560
ttcttgagac atcgtggcgg atgtatggag cgagacgatt tcctgttacc agtggctgct 16620
gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 16680
gcgcagcggt cgggctgaac aggacaatgg tcaccgacga cggtcaccgc tattcagcac 16740
agaatggccc aacctgagtt ctgctatcaa tggcctattc cgcgtcgcca gcccgacttg 16800
ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 16860
acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg ccccccaagc acgtgtgtcg 16920
ggtcgaacct cgcttgctgg atgtggcttg actctatgga tgtcgcactc gatactcttt 16980
cgcggtgcga agggcttccc agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 17040
caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 17100
tctttccgcc tgtccatagg ccattcgccg tcccagcctt gtcctctcgc gtgctccctc 17160
gaaggtcccc ctttgcggac catagaaata tcaggacagc ggtttcgcca cctctgactt 17220
gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 17280
gcggcctttt tacggttcct ccaaagcggt ggagactgaa ctcgcagcta aaaacactac 17340
gagcagtccc cccgcctcgg ataccttttt gcggtcgttg cgccggaaaa atgccaagga 17400
ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 17460
taaccgtatt accgcctttg agtgagctga taccgctcgc ccggaaaacg accggaaaac 17520
gagtgtacaa gaaaggacgc aataggggac taagacacct attggcataa tggcggaaac 17580
tcactcgact atggcgagcg cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 17640
aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 17700
gcgtcggctt gctggctcgc gtcgctcagt cactcgctcc ttcgccttct cgcgggttat 17760
gcgtttggcg gagaggggcg cgcaaccggc taagtaatta gcagctggca cgacaggttt 17820
cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag 17880
gcaccccagg ctttacactt cgtcgaccgt gctgtccaaa gggctgacct ttcgcccgtc 17940
actcgcgttg cgttaattac actcaatcga gtgagtaatc cgtggggtcc gaaatgtgaa 18000
tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa 18060
cagctatgac catgattacg ccaagctcga aattaaccct atacgaaggc cgagcataca 18120
acacacctta acactcgcct attgttaaag tgtgtccttt gtcgatactg gtactaatgc 18180
ggttcgagct ttaattggga cactaaaggg aacaaaagct ggagctccac cgcggtggcg 18240
gcctcgaggt cgagatccgg tcgaccagca accatagtcc cgcccctaac tccgcccatc 18300
gtgatttccc ttgttttcga cctcgaggtg gcgccaccgc cggagctcca gctctaggcc 18360
agctggtcgt tggtatcagg gcggggattg aggcgggtag ccgcccctaa ctccgcccag 18420
ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc 18480
cgcctcggcc tctgagctat ggcggggatt gaggcgggtc aaggcgggta agaggcgggg 18540
taccgactga ttaaaaaaaa taaatacgtc tccggctccg gcggagccgg agactcgata 18600
tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttcgacggt 18660
atcgattggc tcatgtccaa cattaccgcc atgttgacat aggtcttcat cactcctccg 18720
aaaaaacctc cggatccgaa aacgtttttc gaagctgcca tagctaaccg agtacaggtt 18780
gtaatggcgg tacaactgta tgattattga ctagttatta atagtaatca attacggggt 18840
cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc 18900
actaataact gatcaataat tatcattagt taatgcccca gtaatcaagt atcgggtata 18960
tacctcaagg cgcaatgtat tgaatgccat ttaccgggcg ctggctgacc gcccaacgac 19020
ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 19080
cattgacgtc aatgggtgga gaccgactgg cgggttgctg ggggcgggta actgcagtta 19140
ttactgcata caagggtatc attgcggtta tccctgaaag gtaactgcag ttacccacct 19200
gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 19260
ccctattgac gtcaatgacg gtaaatggcc cgcctggcat cataaatgcc atttgacggg 19320
tgaaccgtca tgtagttcac atagtatacg gttcatgcgg gggataactg cagttactgc 19380
catttaccgg gcggaccgta tatgcccagt acatgacctt atgggacttt cctacttggc 19440
agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca 19500
atacgggtca tgtactggaa taccctgaaa ggatgaaccg tcatgtagat gcataatcag 19560
tagcgataat ggtaccacta cgccaaaacc gtcatgtagt atgggcgtgg atagcggttt 19620
gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac 19680
caaaatcaac gggactttcc tacccgcacc tatcgccaaa ctgagtgccc ctaaaggttc 19740
agaggtgggg taactgcagt taccctcaaa caaaaccgtg gttttagttg ccctgaaagg 19800
aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggaattc 19860
ggagtggcga gccctcagat cctgcatata agcagctgct ttttacagca ttgttgaggc 19920
ggggtaactg cgtttacccg ccatccgcac atgccttaag cctcaccgct cgggagtcta 19980
ggacgtatat tcgtcgacga ttttgcctgt actgggtctc tctgaaaacg gacatgaccc 20040
agagagac                                                          20048

<210> 55
<211> 19388
<212> DNA
<213> Artificial Sequence

<220> 
<223> Ce7scFv-IgG4hinge-CH3-CD28tm/4-1BB-zeta-T2A-EGFRt-
      epHIV7 (intermediate)

<400> 55
gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 60
tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc caatctggtc tagactcgga 120
ccctcgagag accgattgat cccttgggtg acgaattcgg agttatttcg aacggaactc 180
acgaagttca tcacacacgg cgtctgttgt gtgactctgg taactagaga tccctcagac 240
ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa 300
gcagacaaca cactgagacc attgatctct agggagtctg ggaaaatcag tcacaccttt 360
tagagatcgt caccgcgggc ttgtccctga actttcgctt agggaaacca gaggagctct 420
ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg 480
tgagtacgcc aaaaattttg tccctttggt ctcctcgaga gagctgcgtc ctgagccgaa 540
cgacttcgcg cgtgccgttc tccgctcccc gccgctgacc actcatgcgg tttttaaaac 600
actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 660
attagatcga tgggaaaaaa ttcggttaag gccaggggga tgatcgcctc cgatcttcct 720
ctctctaccc acgctctcgc agtcataatt cgccccctct taatctagct accctttttt 780
aagccaattc cggtccccct aagaaaaaat ataaattaaa acatatagta tgggcaagca 840
gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac 900
ttctttttta tatttaattt tgtatatcat acccgttcgt ccctcgatct tgctaagcgt 960
caattaggac cggacaatct ttgtagtctt ccgacatctg aaatactggg acagctacaa 1020
ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc 1080
tattgtgtgc atcaaaggat tttatgaccc tgtcgatgtt ggtagggaag tctgtcctag 1140
tcttcttgaa tctagtaata tattatgtca tcgttgggag ataacacacg tagtttccta 1200
agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa 1260
gaaaaaagca cagcaagcag cagctgacac aggacacagc tctctatttt ctgtggttcc 1320
ttcgaaatct gttctatctc cttctcgttt tgttttcatt cttttttcgt gtcgttcgtc 1380
gtcgactgtg tcctgtgtcg aatcaggtca gccaaaatta ccctatagtg cagaacatcc 1440
aggggcaaat ggtacatcag gccatatcac ctagaacttt aaatgcatgg gtaaaagtag 1500
ttagtccagt cggttttaat gggatatcac gtcttgtagg tccccgttta ccatgtagtc 1560
cggtatagtg gatcttgaaa tttacgtacc cattttcatc tagaagagaa ggctttcagc 1620
ccagaagtga tacccatgtt ttcagcatta tcagaaggag ccaccccaca agatttaaac 1680
accatgctaa acacagtggg atcttctctt ccgaaagtcg ggtcttcact atgggtacaa 1740
aagtcgtaat agtcttcctc ggtggggtgt tctaaatttg tggtacgatt tgtgtcaccc 1800
gggacatcaa gcagccatgc aaatgttaaa agagaccatc aatgaggaag ctgcaggcaa 1860
agagaagagt ggtgcagaga gaaaaaagag cagtgggaat ccctgtagtt cgtcggtacg 1920
tttacaattt tctctggtag ttactccttc gacgtccgtt tctcttctca ccacgtctct 1980
cttttttctc gtcaccctta aggagctttg ttccttgggt tcttgggagc agcaggaagc 2040
actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata 2100
tcctcgaaac aaggaaccca agaaccctcg tcgtccttcg tgatacccgc gtcgcagtta 2160
ctgcgactgc catgtccggt ctgttaataa cagaccatat gtgcagcagc agaacaattt 2220
gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 2280
gctccaggca agaatcctgg cacgtcgtcg tcttgttaaa cgactcccga taactccgcg 2340
ttgtcgtaga caacgttgag tgtcagaccc cgtagttcgt cgaggtccgt tcttaggacc 2400
ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac 2460
tcatttgcac cactgctgtg ccttggatct acaaatggca gacacctttc tatggatttc 2520
ctagttgtcg aggaccccta aaccccaacg agaccttttg agtaaacgtg gtgacgacac 2580
ggaacctaga tgtttaccgt gtattcatcc acaattttaa aagaaaaggg gggattgggg 2640
ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat 2700
cataagtagg tgttaaaatt ttcttttccc ccctaacccc ccatgtcacg tcccctttct 2760
tatcatctgt attatcgttg tctgtatgtt tgatttctta tacaaaaaca aattacaaaa 2820
attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttgggg atcaattgca 2880
tgaagaatct gcttagggtt atgtttttgt ttaatgtttt taagttttaa aagcccaaat 2940
aatgtccctg tcgtctctag gtcaaacccc tagttaacgt acttcttaga cgaatcccaa 3000
aggcgttttg cgctgcttcg cgaggatctg cgatcgctcc ggtgcccgtc agtgggcaga 3060
gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg tccgcaaaac gcgacgaagc 3120
gctcctagac gctagcgagg ccacgggcag tcacccgtct cgcgtgtagc gggtgtcagg 3180
ggctcttcaa cccccctccc gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg 3240
taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac 3300
cagccgttaa cttggccacg gatctcttcc accgcgcccc atttgaccct ttcactacag 3360
cacatgaccg aggcggaaaa agggctccca ccccctcttg cgtatataag tgcagtagtc 3420
gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 3480
gctcgcatct ctccttcacg gcatatattc acgtcatcag cggcacttgc aagaaaaagc 3540
gttgcccaaa cggcggtctt gtgtcgactt cgaagctccc cgagcgtaga gaggaagtgc 3600
cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc 3660
cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta gcgggcggcg ggatggactc 3720
cggcggtagg tgcggccaac tcagcgcaag acggcggagg gcggacacca cggaggactt 3780
gacgcaggcg gcagatccat agtttaaagc tcaggtcgag accgggcctt tgtccggcgc 3840
tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc 3900
tcaaatttcg agtccagctc tggcccggaa acaggccgcg agggaacctc ggatggatct 3960
gagtcggccg agaggtgcga aacggactgg gacgaacgag aactctacgt ctttgtttcg 4020
ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacggc tagcgccgcc 4080
accatgctgc tgctggtgac ttgagatgca gaaacaaagc aaaagacaag acgcggcaat 4140
gtctaggttc gacactggcc gcggatgccg atcgcggcgg tggtacgacg acgaccactg 4200
cagcctgctg ctgtgcgagc tgccccaccc cgcctttctg ctgatccccc aggtgcagct 4260
gcagcagcct ggcgccgagc tggtgaagcc aggcgccagc gtcggacgac gacacgctcg 4320
acggggtggg gcggaaagac gactaggggg tccacgtcga cgtcgtcgga ccgcggctcg 4380
accacttcgg tccgcggtcg gtgaagctgt cctgcaaggc cagcggctac accttcaccg 4440
gctactggat gcactgggtg aagcagagac ccggccacgg cctggaatgg atcggcgaga 4500
cacttcgaca ggacgttccg gtcgccgatg tggaagtggc cgatgaccta cgtgacccac 4560
ttcgtctctg ggccggtgcc ggaccttacc tagccgctct tcaaccccag caacggccgg 4620
accaactaca acgagcggtt caagagcaag gccaccctga ccgtggacaa gagcagcacc 4680
accgccttca tgcagctgtc agttggggtc gttgccggcc tggttgatgt tgctcgccaa 4740
gttctcgttc cggtgggact ggcacctgtt ctcgtcgtgg tggcggaagt acgtcgacag 4800
cggcctgacc agcgaggaca gcgccgtgta cttctgcgcc agggactact acggcaccag 4860
ctacaacttc gactactggg gccagggcac cacactgacc gccggactgg tcgctcctgt 4920
cgcggcacat gaagacgcgg tccctgatga tgccgtggtc gatgttgaag ctgatgaccc 4980
cggtcccgtg gtgtgactgg gtgagcagcg gcggaggggg ctctggcggc ggaggatctg 5040
ggggaggggg cagcgacatc cagatgaccc agagcagcag cagcttcagc gtgagcctgg 5100
cactcgtcgc cgcctccccc gagaccgccg cctcctagac cccctccccc gtcgctgtag 5160
gtctactggg tctcgtcgtc gtcgaagtcg cactcggacc gcgaccgggt gaccatcacc 5220
tgtaaggcca acgaggacat caacaaccgg ctggcctggt atcagcagac ccccggcaac 5280
agccccaggc tgctgatcag cgctggccca ctggtagtgg acattccggt tgctcctgta 5340
gttgttggcc gaccggacca tagtcgtctg ggggccgttg tcggggtccg acgactagtc 5400
cggcgccacc aacctggtga ccggcgtgcc cagccggttt agcggcagcg gctccggcaa 5460
ggactacacc ctgaccatca caagcctgca ggccgaggac gccgcggtgg ttggaccact 5520
ggccgcacgg gtcggccaaa tcgccgtcgc cgaggccgtt cctgatgtgg gactggtagt 5580
gttcggacgt ccggctcctg ttcgccacct actactgcca gcagtactgg tccaccccct 5640
tcaccttcgg cagcggcacc gagctggaaa tcaaagaatc taagtacgga ccgccctgcc 5700
aagcggtgga tgatgacggt cgtcatgacc aggtggggga agtggaagcc gtcgccgtgg 5760
ctcgaccttt agtttcttag attcatgcct ggcgggacgg ccccttgccc tggccagcct 5820
agagaacccc aggtgtacac cctgcctccc agccaggaag agatgaccaa gaaccaggtg 5880
tccctgacct gcctggtcaa ggggaacggg accggtcgga tctcttgggg tccacatgtg 5940
ggacggaggg tcggtccttc tctactggtt cttggtccac agggactgga cggaccagtt 6000
aggcttctac cccagcgata tcgccgtgga atgggagagc aacggccagc ccgagaacaa 6060
ctacaagacc accccccctg tgctggacag cgacggcagc tccgaagatg gggtcgctat 6120
agcggcacct taccctctcg ttgccggtcg ggctcttgtt gatgttctgg tgggggggac 6180
acgacctgtc gctgccgtcg ttcttcctgt actcccggct gaccgtggac aagagccggt 6240
ggcaggaagg caacgtcttc agctgcagcg tgatgcacga ggccctgcac aaccactaca 6300
aagaaggaca tgagggccga ctggcacctg ttctcggcca ccgtccttcc gttgcagaag 6360
tcgacgtcgc actacgtgct ccgggacgtg ttggtgatgt cccagaagtc cctgagcctg 6420
agcctgggca agatgttctg ggtgctggtg gtggtcggag gcgtgctggc ctgctacagc 6480
ctgctggtca ccgtggcctt gggtcttcag ggactcggac tcggacccgt tctacaagac 6540
ccacgaccac caccagcctc cgcacgaccg gacgatgtcg gacgaccagt ggcaccggaa 6600
catcatcttt tgggtgaaac ggggcagaaa gaaactcctg tatatattca aacaaccatt 6660
tatgagacca gtacaaacta ctcaagagga agatggctgt gtagtagaaa acccactttg 6720
ccccgtcttt ctttgaggac atatataagt ttgttggtaa atactctggt catgtttgat 6780
gagttctcct tctaccgaca agctgccgat ttccagaaga agaagaagga ggatgtgaac 6840
tgcgggtgaa gttcagcaga agcgccgacg cccctgccta ccagcagggc cagaatcagc 6900
tcgacggcta aaggtcttct tcttcttcct cctacacttg acgcccactt caagtcgtct 6960
tcgcggctgc ggggacggat ggtcgtcccg gtcttagtcg tgtacaacga gctgaacctg 7020
ggcagaaggg aagagtacga cgtcctggat aagcggagag gccgggaccc tgagatgggc 7080
ggcaagcctc ggcggaagaa acatgttgct cgacttggac ccgtcttccc ttctcatgct 7140
gcaggaccta ttcgcctctc cggccctggg actctacccg ccgttcggag ccgccttctt 7200
cccccaggaa ggcctgtata acgaactgca gaaagacaag atggccgagg cctacagcga 7260
gatcggcatg aagggcgagc ggaggcgggg caagggccac gggggtcctt ccggacatat 7320
tgcttgacgt ctttctgttc taccggctcc ggatgtcgct ctagccgtac ttcccgctcg 7380
cctccgcccc gttcccggtg gacggcctgt atcagggcct gtccaccgcc accaaggata 7440
cctacgacgc cctgcacatg caggccctgc ccccaaggct cgagggcggc ggagagggca 7500
ctgccggaca tagtcccgga caggtggcgg tggttcctat ggatgctgcg ggacgtgtac 7560
gtccgggacg ggggttccga gctcccgccg cctctcccgt gaggaagtct tctaacatgc 7620
ggtgacgtgg aggagaatcc cggccctagg atgcttctcc tggtgacaag ccttctgctc 7680
tgtgagttac cacacccagc ctccttcaga agattgtacg ccactgcacc tcctcttagg 7740
gccgggatcc tacgaagagg accactgttc ggaagacgag acactcaatg gtgtgggtcg 7800
attcctcctg atcccacgca aagtgtgtaa cggaataggt attggtgaat ttaaagactc 7860
actctccata aatgctacga atattaaaca cttcaaaaac taaggaggac tagggtgcgt 7920
ttcacacatt gccttatcca taaccactta aatttctgag tgagaggtat ttacgatgct 7980
tataatttgt gaagtttttg tgcacctcca tcagtggcga tctccacatc ctgccggtgg 8040
catttagggg tgactccttc acacatactc ctcctctgga tccacaggaa ctggatattc 8100
acgtggaggt agtcaccgct agaggtgtag gacggccacc gtaaatcccc actgaggaag 8160
tgtgtatgag gaggagacct aggtgtcctt gacctataag tgaaaaccgt aaaggaaatc 8220
acagggtttt tgctgattca ggcttggcct gaaaacagga cggacctcca tgcctttgag 8280
aacctagaaa tcatacgcgg acttttggca tttcctttag tgtcccaaaa acgactaagt 8340
ccgaaccgga cttttgtcct gcctggaggt acggaaactc ttggatcttt agtatgcgcc 8400
caggaccaag caacatggtc agttttctct tgcagtcgtc agcctgaaca taacatcctt 8460
gggattacgc tccctcaagg agataagtga tggagatgtg gtcctggttc gttgtaccag 8520
tcaaaagaga acgtcagcag tcggacttgt attgtaggaa ccctaatgcg agggagttcc 8580
tctattcact acctctacac ataatttcag gaaacaaaaa tttgtgctat gcaaatacaa 8640
taaactggaa aaaactgttt gggacctccg gtcagaaaac caaaattata agcaacagag 8700
tattaaagtc ctttgttttt aaacacgata cgtttatgtt atttgacctt ttttgacaaa 8760
ccctggaggc cagtcttttg gttttaatat tcgttgtctc gtgaaaacag ctgcaaggcc 8820
acaggccagg tctgccatgc cttgtgctcc cccgagggct gctggggccc ggagcccagg 8880
gactgcgtct cttgccggaa cacttttgtc gacgttccgg tgtccggtcc agacggtacg 8940
gaacacgagg gggctcccga cgaccccggg cctcgggtcc ctgacgcaga gaacggcctt 9000
tgtcagccga ggcagggaat gcgtggacaa gtgcaacctt ctggagggtg agccaaggga 9060
gtttgtggag aactctgagt gcatacagtg ccacccagag acagtcggct ccgtccctta 9120
cgcacctgtt cacgttggaa gacctcccac tcggttccct caaacacctc ttgagactca 9180
cgtatgtcac ggtgggtctc tgcctgcctc aggccatgaa catcacctgc acaggacggg 9240
gaccagacaa ctgtatccag tgtgcccact acattgacgg cccccactgc gtcaagacct 9300
acggacggag tccggtactt gtagtggacg tgtcctgccc ctggtctgtt gacataggtc 9360
acacgggtga tgtaactgcc gggggtgacg cagttctgga gcccggcagg agtcatggga 9420
gaaaacaaca ccctggtctg gaagtacgca gacgccggcc atgtgtgcca cctgtgccat 9480
ccaaactgca cctacggatg cgggccgtcc tcagtaccct cttttgttgt gggaccagac 9540
cttcatgcgt ctgcggccgg tacacacggt ggacacggta ggtttgacgt ggatgcctac 9600
cactgggcca ggtcttgaag gctgtccaac gaatgggcct aagatcccgt ccatcgccac 9660
tgggatggtg ggggccctcc tcttgctgct ggtggtggcc gtgacccggt ccagaacttc 9720
cgacaggttg cttacccgga ttctagggca ggtagcggtg accctaccac ccccgggagg 9780
agaacgacga ccaccaccgg ctggggatcg gcctcttcat gtgagcggcc gctctagacc 9840
cgggctgcag gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg 9900
gacccctagc cggagaagta cactcgccgg cgagatctgg gcccgacgtc cttaagctat 9960
agttcgaata gctattagtt ggagacctaa tgttttaaac tgaaagattg actggtattc 10020
ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 10080
ctattgcttc ccgtatggct actttctaac tgaccataag aattgataca acgaggaaaa 10140
tgcgatacac ctatgcgacg aaattacgga aacatagtac gataacgaag ggcataccga 10200
ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc 10260
gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg aagtaaaaga ggaggaacat 10320
atttaggacc aacgacagag aaatactcct caacaccggg caacagtccg ttgcaccgca 10380
ccacacgtga cacaaacgac acgcaacccc cactggttgg ggcattgcca ccacctgtca 10440
gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 10500
tgcgttgggg gtgaccaacc ccgtaacggt ggtggacagt cgaggaaagg ccctgaaagc 10560
gaaaggggga gggataacgg tgccgccttg agtagcggcg ctgccttgcc cgctgctgga 10620
caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct 10680
ttccttggct gctcgcctgt gacggaacgg gcgacgacct gtccccgagc cgacaacccg 10740
tgactgttaa ggcaccacaa cagccccttt agtagcagga aaggaaccga cgagcggaca 10800
gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca 10860
gcggaccttc cttcccgcgg cctgctgccg gctctgcggc caacggtgga cctaagacgc 10920
gccctgcagg aagacgatgc agggaagccg ggagttaggt cgcctggaag gaagggcgcc 10980
ggacgacggc cgagacgccg ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat 11040
ctccctttgg gccgcctccc cgcatcgata ccgtcgacta gccgtacctt taagaccaat 11100
gagaaggcgc agaagcggaa gcgggagtct gctcagccta gagggaaacc cggcggaggg 11160
gcgtagctat ggcagctgat cggcatggaa attctggtta gacttacaag gcagctgtag 11220
atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaaagaa 11280
gacaagatct gctttttgcc ctgaatgttc cgtcgacatc tagaatcggt gaaaaatttt 11340
cttttccccc ctgaccttcc cgattaagtg agggtttctt ctgttctaga cgaaaaacgg 11400
tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 11460
aacccactgc ttaagcctca ataaagcttg ccttgagtgc acatgaccca gagagaccaa 11520
tctggtctag actcggaccc tcgagagacc gattgatccc ttgggtgacg aattcggagt 11580
tatttcgaac ggaactcacg ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 11640
ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagaa ttcgatatca 11700
aagttcatca cacacgggca gacaacacac tgagaccatt gatctctagg gagtctggga 11760
aaatcagtca caccttttag agatcgtctt aagctatagt agcttatcga taccgtcgac 11820
ctcgaggggg ggcccggtac ccaattcgcc ctatagtgag tcgtattaca attcactggc 11880
cgtcgtttta caacgtcgtg tcgaatagct atggcagctg gagctccccc ccgggccatg 11940
ggttaagcgg gatatcactc agcataatgt taagtgaccg gcagcaaaat gttgcagcac 12000
actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca 12060
gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc tgaccctttt gggaccgcaa 12120
tgggttgaat tagcggaacg tcgtgtaggg ggaaagcggt cgaccgcatt atcgcttctc 12180
cgggcgtggc tagcgggaag ccaacagttg cgcagcctga atggcgaatg gaaattgtaa 12240
gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc 12300
ggttgtcaac gcgtcggact taccgcttac ctttaacatt cgcaattata aaacaatttt 12360
aagcgcaatt taaaaacaat ttagtcgagt aaaaaattgg aataggccga aatcggcaaa 12420
atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac 12480
aagagtccac tattaaagaa ttatccggct ttagccgttt tagggaatat ttagttttct 12540
tatctggctc tatcccaact cacaacaagg tcaaaccttg ttctcaggtg ataatttctt 12600
cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga 12660
accatcaccc taatcaagtt ttttggggtc gaggtgccgt gcacctgagg ttgcagtttc 12720
ccgctttttg gcagatagtc ccgctaccgg gtgatgcact tggtagtggg attagttcaa 12780
aaaaccccag ctccacggca aaagcactaa atcggaaccc taaagggagc ccccgattta 12840
gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag 12900
tttcgtgatt tagccttggg atttccctcg ggggctaaat ctcgaactgc ccctttcggc 12960
cgcttgcacc gctctttcct tcccttcttt cgctttcctc cgggcgctag ggcgctggca 13020
agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag 13080
ggcgcgtcag gtggcacttt gcccgcgatc ccgcgaccgt tcacatcgcc agtgcgacgc 13140
gcattggtgg tgtgggcggc gcgaattacg cggcgatgtc ccgcgcagtc caccgtgaaa 13200
tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 13260
tccgctcatg agacaataac cctgataaat gcttcaataa agccccttta cacgcgcctt 13320
ggggataaac aaataaaaag atttatgtaa gtttatacat aggcgagtac tctgttattg 13380
ggactattta cgaagttatt tattgaaaaa ggaagagtat gagtattcaa catttccgtg 13440
tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 13500
ataacttttt ccttctcata ctcataagtt gtaaaggcac agcgggaata agggaaaaaa 13560
cgccgtaaaa cggaaggaca aaaacgagtg ggtctttgcg tggtgaaagt aaaagatgct 13620
gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 13680
cttgagagtt ttcgccccga accactttca ttttctacga cttctagtca acccacgtgc 13740
tcacccaatg tagcttgacc tagagttgtc gccattctag gaactctcaa aagcggggct 13800
agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 13860
tattgacgcc gggcaagagc aactcggtcg ccgcatacac tcttgcaaaa ggttactact 13920
cgtgaaaatt tcaagacgat acaccgcgcc ataatagggc ataactgcgg cccgttctcg 13980
ttgagccagc ggcgtatgtg tattctcaga atgacttggt tgagtactca ccagtcacag 14040
aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 14100
ataagagtct tactgaacca actcatgagt ggtcagtgtc ttttcgtaga atgcctaccg 14160
tactgtcatt ctcttaatac gtcacgacgg tattggtact gtgataacac tgcggccaac 14220
ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 14280
gatcatgtaa ctcgccttga cactattgtg acgccggttg aatgaagact gttgctagcc 14340
tcctggcttc ctcgattggc gaaaaaacgt gttgtacccc ctagtacatt gagcggaact 14400
tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 14460
tgtagcaatg gcaacaacgt tgcgcaaact attaactggc agcaaccctt ggcctcgact 14520
tacttcggta tggtttgctg ctcgcactgt ggtgctacgg acatcgttac cgttgttgca 14580
acgcgtttga taattgaccg gaactactta ctctagcttc ccggcaacaa ttaatagact 14640
ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 14700
cttgatgaat gagatcgaag ggccgttgtt aattatctga cctacctccg cctatttcaa 14760
cgtcctggtg aagacgcgag ccgggaaggc cgaccgacca ttattgctga taaatctgga 14820
gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 14880
cgtatcgtag ttatctacac aataacgact atttagacct cggccactcg cacccagagc 14940
gccatagtaa cgtcgtgacc ccggtctacc attcgggagg gcatagcatc aatagatgtg 15000
gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 15060
actgattaag cattggtaac tgtcagacca agtttactca ctgcccctca gtccgttgat 15120
acctacttgc tttatctgtc tagcgactct atccacggag tgactaattc gtaaccattg 15180
acagtctggt tcaaatgagt tatatacttt agattgattt aaaacttcat ttttaattta 15240
aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 15300
atatatgaaa tctaactaaa ttttgaagta aaaattaaat tttcctagat ccacttctag 15360
gaaaaactat tagagtactg gttttaggga attgcactca tttcgttcca ctgagcgtca 15420
gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 15480
tgcttgcaaa caaaaaaacc aaagcaaggt gactcgcagt ctggggcatc ttttctagtt 15540
tcctagaaga actctaggaa aaaaagacgc gcattagacg acgaacgttt gtttttttgg 15600
accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 15660
aactggcttc agcagagcgc agataccaaa tactgttctt tggcgatggt cgccaccaaa 15720
caaacggcct agttctcgat ggttgagaaa aaggcttcca ttgaccgaag tcgtctcgcg 15780
tctatggttt atgacaagaa ctagtgtagc cgtagttagg ccaccacttc aagaactctg 15840
tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 15900
gatcacatcg gcatcaatcc ggtggtgaag ttcttgagac atcgtggcgg atgtatggag 15960
cgagacgatt aggacaatgg tcaccgacga cggtcaccgc ataagtcgtg tcttaccggg 16020
ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 16080
tgcacacagc ccagcttgga tattcagcac agaatggccc aacctgagtt ctgctatcaa 16140
tggcctattc cgcgtcgcca gcccgacttg ccccccaagc acgtgtgtcg ggtcgaacct 16200
gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 16260
tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc cgcttgctgg atgtggcttg 16320
actctatgga tgtcgcactc gatactcttt cgcggtgcga agggcttccc tctttccgcc 16380
tgtccatagg ccattcgccg agggtcggaa caggagagcg cacgagggag cttccagggg 16440
gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 16500
tcccagcctt gtcctctcgc gtgctccctc gaaggtcccc ctttgcggac catagaaata 16560
tcaggacagc ccaaagcggt ggagactgaa ctcgcagcta ttttgtgatg ctcgtcaggg 16620
gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 16680
tggccttttg ctcacatgtt aaaacactac gagcagtccc cccgcctcgg ataccttttt 16740
gcggtcgttg cgccggaaaa atgccaagga ccggaaaacg accggaaaac gagtgtacaa 16800
ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 16860
taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gaaaggacgc aataggggac 16920
taagacacct attggcataa tggcggaaac tcactcgact atggcgagcg gcgtcggctt 16980
gctggctcgc gtcgctcagt gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc 17040
ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 17100
cactcgctcc ttcgccttct cgcgggttat gcgtttggcg gagaggggcg cgcaaccggc 17160
taagtaatta cgtcgaccgt gctgtccaaa gggctgacct aagcgggcag tgagcgcaac 17220
gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg 17280
gctcgtatgt tgtgtggaat ttcgcccgtc actcgcgttg cgttaattac actcaatcga 17340
gtgagtaatc cgtggggtcc gaaatgtgaa atacgaaggc cgagcataca acacacctta 17400
tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcga 17460
aattaaccct cactaaaggg aacaaaagct ggagctccac acactcgcct attgttaaag 17520
tgtgtccttt gtcgatactg gtactaatgc ggttcgagct ttaattggga gtgatttccc 17580
ttgttttcga cctcgaggtg cgcggtggcg gcctcgaggt cgagatccgg tcgaccagca 17640
accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 17700
gcgccaccgc cggagctcca gctctaggcc agctggtcgt tggtatcagg gcggggattg 17760
aggcgggtag ggcggggatt gaggcgggtc aaggcgggta tctccgcccc atggctgact 17820
aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta 17880
gtgaggaggc ttttttggag agaggcgggg taccgactga ttaaaaaaaa taaatacgtc 17940
tccggctccg gcggagccgg agactcgata aggtcttcat cactcctccg aaaaaacctc 18000
gcctaggctt ttgcaaaaag cttcgacggt atcgattggc tcatgtccaa cattaccgcc 18060
atgttgacat tgattattga ctagttatta atagtaatca cggatccgaa aacgtttttc 18120
gaagctgcca tagctaaccg agtacaggtt gtaatggcgg tacaactgta actaataact 18180
gatcaataat tatcattagt attacggggt cattagttca tagcccatat atggagttcc 18240
gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat 18300
taatgcccca gtaatcaagt atcgggtata tacctcaagg cgcaatgtat tgaatgccat 18360
ttaccgggcg gaccgactgg cgggttgctg ggggcgggta tgacgtcaat aatgacgtat 18420
gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg 18480
taaactgccc acttggcagt actgcagtta ttactgcata caagggtatc attgcggtta 18540
tccctgaaag gtaactgcag ttacccacct cataaatgcc atttgacggg tgaaccgtca 18600
acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc 18660
cgcctggcat tatgcccagt acatgacctt atgggacttt tgtagttcac atagtatacg 18720
gttcatgcgg gggataactg cagttactgc catttaccgg gcggaccgta atacgggtca 18780
tgtactggaa taccctgaaa cctacttggc agtacatcta cgtattagtc atcgctatta 18840
ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg 18900
ggatgaaccg tcatgtagat gcataatcag tagcgataat ggtaccacta cgccaaaacc 18960
gtcatgtagt tacccgcacc tatcgccaaa ctgagtgccc gatttccaag tctccacccc 19020
attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt 19080
aacaactccg ccccattgac ctaaaggttc agaggtgggg taactgcagt taccctcaaa 19140
caaaaccgtg gttttagttg ccctgaaagg ttttacagca ttgttgaggc ggggtaactg 19200
gcaaatgggc ggtaggcgtg tacggaattc ggagtggcga gccctcagat cctgcatata 19260
agcagctgct ttttgcctgt actgggtctc tctgcgttta cccgccatcc gcacatgcct 19320
taagcctcac cgctcgggag tctaggacgt atattcgtcg acgaaaaacg gacatgaccc 19380
agagagac                                                          19388

<210> 56
<211> 18746
<212> DNA
<213> Artificial Sequence

<220> 
<223> Ce7scFv-IgG4hinge-CD28tm/4-1BB-zeta-T2A-EGFRt-epHI
      V7

<400> 56
gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 60
tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc caatctggtc tagactcgga 120
ccctcgagag accgattgat cccttgggtg acgaattcgg agttatttcg aacggaactc 180
acgaagttca tcacacacgg cgtctgttgt gtgactctgg taactagaga tccctcagac 240
ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa 300
gcagacaaca cactgagacc attgatctct agggagtctg ggaaaatcag tcacaccttt 360
tagagatcgt caccgcgggc ttgtccctga actttcgctt agggaaacca gaggagctct 420
ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg 480
tgagtacgcc aaaaattttg tccctttggt ctcctcgaga gagctgcgtc ctgagccgaa 540
cgacttcgcg cgtgccgttc tccgctcccc gccgctgacc actcatgcgg tttttaaaac 600
actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 660
attagatcga tgggaaaaaa ttcggttaag gccaggggga tgatcgcctc cgatcttcct 720
ctctctaccc acgctctcgc agtcataatt cgccccctct taatctagct accctttttt 780
aagccaattc cggtccccct aagaaaaaat ataaattaaa acatatagta tgggcaagca 840
gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac 900
ttctttttta tatttaattt tgtatatcat acccgttcgt ccctcgatct tgctaagcgt 960
caattaggac cggacaatct ttgtagtctt ccgacatctg aaatactggg acagctacaa 1020
ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc 1080
tattgtgtgc atcaaaggat tttatgaccc tgtcgatgtt ggtagggaag tctgtcctag 1140
tcttcttgaa tctagtaata tattatgtca tcgttgggag ataacacacg tagtttccta 1200
agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa 1260
gaaaaaagca cagcaagcag cagctgacac aggacacagc tctctatttt ctgtggttcc 1320
ttcgaaatct gttctatctc cttctcgttt tgttttcatt cttttttcgt gtcgttcgtc 1380
gtcgactgtg tcctgtgtcg aatcaggtca gccaaaatta ccctatagtg cagaacatcc 1440
aggggcaaat ggtacatcag gccatatcac ctagaacttt aaatgcatgg gtaaaagtag 1500
ttagtccagt cggttttaat gggatatcac gtcttgtagg tccccgttta ccatgtagtc 1560
cggtatagtg gatcttgaaa tttacgtacc cattttcatc tagaagagaa ggctttcagc 1620
ccagaagtga tacccatgtt ttcagcatta tcagaaggag ccaccccaca agatttaaac 1680
accatgctaa acacagtggg atcttctctt ccgaaagtcg ggtcttcact atgggtacaa 1740
aagtcgtaat agtcttcctc ggtggggtgt tctaaatttg tggtacgatt tgtgtcaccc 1800
gggacatcaa gcagccatgc aaatgttaaa agagaccatc aatgaggaag ctgcaggcaa 1860
agagaagagt ggtgcagaga gaaaaaagag cagtgggaat ccctgtagtt cgtcggtacg 1920
tttacaattt tctctggtag ttactccttc gacgtccgtt tctcttctca ccacgtctct 1980
cttttttctc gtcaccctta aggagctttg ttccttgggt tcttgggagc agcaggaagc 2040
actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata 2100
tcctcgaaac aaggaaccca agaaccctcg tcgtccttcg tgatacccgc gtcgcagtta 2160
ctgcgactgc catgtccggt ctgttaataa cagaccatat gtgcagcagc agaacaattt 2220
gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 2280
gctccaggca agaatcctgg cacgtcgtcg tcttgttaaa cgactcccga taactccgcg 2340
ttgtcgtaga caacgttgag tgtcagaccc cgtagttcgt cgaggtccgt tcttaggacc 2400
ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac 2460
tcatttgcac cactgctgtg ccttggatct acaaatggca gacacctttc tatggatttc 2520
ctagttgtcg aggaccccta aaccccaacg agaccttttg agtaaacgtg gtgacgacac 2580
ggaacctaga tgtttaccgt gtattcatcc acaattttaa aagaaaaggg gggattgggg 2640
ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat 2700
cataagtagg tgttaaaatt ttcttttccc ccctaacccc ccatgtcacg tcccctttct 2760
tatcatctgt attatcgttg tctgtatgtt tgatttctta tacaaaaaca aattacaaaa 2820
attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttgggg atcaattgca 2880
tgaagaatct gcttagggtt atgtttttgt ttaatgtttt taagttttaa aagcccaaat 2940
aatgtccctg tcgtctctag gtcaaacccc tagttaacgt acttcttaga cgaatcccaa 3000
aggcgttttg cgctgcttcg cgaggatctg cgatcgctcc ggtgcccgtc agtgggcaga 3060
gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg tccgcaaaac gcgacgaagc 3120
gctcctagac gctagcgagg ccacgggcag tcacccgtct cgcgtgtagc gggtgtcagg 3180
ggctcttcaa cccccctccc gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg 3240
taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac 3300
cagccgttaa cttggccacg gatctcttcc accgcgcccc atttgaccct ttcactacag 3360
cacatgaccg aggcggaaaa agggctccca ccccctcttg cgtatataag tgcagtagtc 3420
gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 3480
gctcgcatct ctccttcacg gcatatattc acgtcatcag cggcacttgc aagaaaaagc 3540
gttgcccaaa cggcggtctt gtgtcgactt cgaagctccc cgagcgtaga gaggaagtgc 3600
cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc 3660
cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta gcgggcggcg ggatggactc 3720
cggcggtagg tgcggccaac tcagcgcaag acggcggagg gcggacacca cggaggactt 3780
gacgcaggcg gcagatccat agtttaaagc tcaggtcgag accgggcctt tgtccggcgc 3840
tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc 3900
tcaaatttcg agtccagctc tggcccggaa acaggccgcg agggaacctc ggatggatct 3960
gagtcggccg agaggtgcga aacggactgg gacgaacgag aactctacgt ctttgtttcg 4020
ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacggc tagcgccgcc 4080
accatgctgc tgctggtgac ttgagatgca gaaacaaagc aaaagacaag acgcggcaat 4140
gtctaggttc gacactggcc gcggatgccg atcgcggcgg tggtacgacg acgaccactg 4200
cagcctgctg ctgtgcgagc tgccccaccc cgcctttctg ctgatccccc aggtgcagct 4260
gcagcagcct ggcgccgagc tggtgaagcc aggcgccagc gtcggacgac gacacgctcg 4320
acggggtggg gcggaaagac gactaggggg tccacgtcga cgtcgtcgga ccgcggctcg 4380
accacttcgg tccgcggtcg gtgaagctgt cctgcaaggc cagcggctac accttcaccg 4440
gctactggat gcactgggtg aagcagagac ccggccacgg cctggaatgg atcggcgaga 4500
cacttcgaca ggacgttccg gtcgccgatg tggaagtggc cgatgaccta cgtgacccac 4560
ttcgtctctg ggccggtgcc ggaccttacc tagccgctct tcaaccccag caacggccgg 4620
accaactaca acgagcggtt caagagcaag gccaccctga ccgtggacaa gagcagcacc 4680
accgccttca tgcagctgtc agttggggtc gttgccggcc tggttgatgt tgctcgccaa 4740
gttctcgttc cggtgggact ggcacctgtt ctcgtcgtgg tggcggaagt acgtcgacag 4800
cggcctgacc agcgaggaca gcgccgtgta cttctgcgcc agggactact acggcaccag 4860
ctacaacttc gactactggg gccagggcac cacactgacc gccggactgg tcgctcctgt 4920
cgcggcacat gaagacgcgg tccctgatga tgccgtggtc gatgttgaag ctgatgaccc 4980
cggtcccgtg gtgtgactgg gtgagcagcg gcggaggggg ctctggcggc ggaggatctg 5040
ggggaggggg cagcgacatc cagatgaccc agagcagcag cagcttcagc gtgagcctgg 5100
cactcgtcgc cgcctccccc gagaccgccg cctcctagac cccctccccc gtcgctgtag 5160
gtctactggg tctcgtcgtc gtcgaagtcg cactcggacc gcgaccgggt gaccatcacc 5220
tgtaaggcca acgaggacat caacaaccgg ctggcctggt atcagcagac ccccggcaac 5280
agccccaggc tgctgatcag cgctggccca ctggtagtgg acattccggt tgctcctgta 5340
gttgttggcc gaccggacca tagtcgtctg ggggccgttg tcggggtccg acgactagtc 5400
cggcgccacc aacctggtga ccggcgtgcc cagccggttt agcggcagcg gctccggcaa 5460
ggactacacc ctgaccatca caagcctgca ggccgaggac gccgcggtgg ttggaccact 5520
ggccgcacgg gtcggccaaa tcgccgtcgc cgaggccgtt cctgatgtgg gactggtagt 5580
gttcggacgt ccggctcctg ttcgccacct actactgcca gcagtactgg tccaccccct 5640
tcaccttcgg cagcggcacc gagctggaaa tcaaagaatc taagtacgga ccgccctgcc 5700
aagcggtgga tgatgacggt cgtcatgacc aggtggggga agtggaagcc gtcgccgtgg 5760
ctcgaccttt agtttcttag attcatgcct ggcgggacgg ccccttgccc tatgttctgg 5820
gtgctggtgg tggtcggagg cgtgctggcc tgctacagcc tgctggtcac cgtggccttc 5880
atcatctttt gggtgaaacg ggggaacggg atacaagacc cacgaccacc accagcctcc 5940
gcacgaccgg acgatgtcgg acgaccagtg gcaccggaag tagtagaaaa cccactttgc 6000
gggcagaaag aaactcctgt atatattcaa acaaccattt atgagaccag tacaaactac 6060
tcaagaggaa gatggctgta gctgccgatt tccagaagaa cccgtctttc tttgaggaca 6120
tatataagtt tgttggtaaa tactctggtc atgtttgatg agttctcctt ctaccgacat 6180
cgacggctaa aggtcttctt gaagaaggag gatgtgaact gcgggtgaag ttcagcagaa 6240
gcgccgacgc ccctgcctac cagcagggcc agaatcagct gtacaacgag ctgaacctgg 6300
cttcttcctc ctacacttga cgcccacttc aagtcgtctt cgcggctgcg gggacggatg 6360
gtcgtcccgg tcttagtcga catgttgctc gacttggacc gcagaaggga agagtacgac 6420
gtcctggata agcggagagg ccgggaccct gagatgggcg gcaagcctcg gcggaagaac 6480
ccccaggaag gcctgtataa cgtcttccct tctcatgctg caggacctat tcgcctctcc 6540
ggccctggga ctctacccgc cgttcggagc cgccttcttg ggggtccttc cggacatatt 6600
cgaactgcag aaagacaaga tggccgaggc ctacagcgag atcggcatga agggcgagcg 6660
gaggcggggc aagggccacg acggcctgta tcagggcctg gcttgacgtc tttctgttct 6720
accggctccg gatgtcgctc tagccgtact tcccgctcgc ctccgccccg ttcccggtgc 6780
tgccggacat agtcccggac tccaccgcca ccaaggatac ctacgacgcc ctgcacatgc 6840
aggccctgcc cccaaggctc gagggcggcg gagagggcag aggaagtctt ctaacatgcg 6900
aggtggcggt ggttcctatg gatgctgcgg gacgtgtacg tccgggacgg gggttccgag 6960
ctcccgccgc ctctcccgtc tccttcagaa gattgtacgc gtgacgtgga ggagaatccc 7020
ggccctagga tgcttctcct ggtgacaagc cttctgctct gtgagttacc acacccagca 7080
ttcctcctga tcccacgcaa cactgcacct cctcttaggg ccgggatcct acgaagagga 7140
ccactgttcg gaagacgaga cactcaatgg tgtgggtcgt aaggaggact agggtgcgtt 7200
agtgtgtaac ggaataggta ttggtgaatt taaagactca ctctccataa atgctacgaa 7260
tattaaacac ttcaaaaact gcacctccat cagtggcgat tcacacattg ccttatccat 7320
aaccacttaa atttctgagt gagaggtatt tacgatgctt ataatttgtg aagtttttga 7380
cgtggaggta gtcaccgcta ctccacatcc tgccggtggc atttaggggt gactccttca 7440
cacatactcc tcctctggat ccacaggaac tggatattct gaaaaccgta aaggaaatca 7500
gaggtgtagg acggccaccg taaatcccca ctgaggaagt gtgtatgagg aggagaccta 7560
ggtgtccttg acctataaga cttttggcat ttcctttagt cagggttttt gctgattcag 7620
gcttggcctg aaaacaggac ggacctccat gcctttgaga acctagaaat catacgcggc 7680
aggaccaagc aacatggtca gtcccaaaaa cgactaagtc cgaaccggac ttttgtcctg 7740
cctggaggta cggaaactct tggatcttta gtatgcgccg tcctggttcg ttgtaccagt 7800
gttttctctt gcagtcgtca gcctgaacat aacatccttg ggattacgct ccctcaagga 7860
gataagtgat ggagatgtga taatttcagg aaacaaaaat caaaagagaa cgtcagcagt 7920
cggacttgta ttgtaggaac cctaatgcga gggagttcct ctattcacta cctctacact 7980
attaaagtcc tttgttttta ttgtgctatg caaatacaat aaactggaaa aaactgtttg 8040
ggacctccgg tcagaaaacc aaaattataa gcaacagagg tgaaaacagc tgcaaggcca 8100
aacacgatac gtttatgtta tttgaccttt tttgacaaac cctggaggcc agtcttttgg 8160
ttttaatatt cgttgtctcc acttttgtcg acgttccggt caggccaggt ctgccatgcc 8220
ttgtgctccc ccgagggctg ctggggcccg gagcccaggg actgcgtctc ttgccggaat 8280
gtcagccgag gcagggaatg gtccggtcca gacggtacgg aacacgaggg ggctcccgac 8340
gaccccgggc ctcgggtccc tgacgcagag aacggcctta cagtcggctc cgtcccttac 8400
cgtggacaag tgcaaccttc tggagggtga gccaagggag tttgtggaga actctgagtg 8460
catacagtgc cacccagagt gcctgcctca ggccatgaac gcacctgttc acgttggaag 8520
acctcccact cggttccctc aaacacctct tgagactcac gtatgtcacg gtgggtctca 8580
cggacggagt ccggtacttg atcacctgca caggacgggg accagacaac tgtatccagt 8640
gtgcccacta cattgacggc ccccactgcg tcaagacctg cccggcagga gtcatgggag 8700
tagtggacgt gtcctgcccc tggtctgttg acataggtca cacgggtgat gtaactgccg 8760
ggggtgacgc agttctggac gggccgtcct cagtaccctc aaaacaacac cctggtctgg 8820
aagtacgcag acgccggcca tgtgtgccac ctgtgccatc caaactgcac ctacggatgc 8880
actgggccag gtcttgaagg ttttgttgtg ggaccagacc ttcatgcgtc tgcggccggt 8940
acacacggtg gacacggtag gtttgacgtg gatgcctacg tgacccggtc cagaacttcc 9000
ctgtccaacg aatgggccta agatcccgtc catcgccact gggatggtgg gggccctcct 9060
cttgctgctg gtggtggccc tggggatcgg cctcttcatg gacaggttgc ttacccggat 9120
tctagggcag gtagcggtga ccctaccacc cccgggagga gaacgacgac caccaccggg 9180
acccctagcc ggagaagtac tgagcggccg ctctagaccc gggctgcagg aattcgatat 9240
caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 9300
actcgccggc gagatctggg cccgacgtcc ttaagctata gttcgaatag ctattagttg 9360
gagacctaat gttttaaaca ctttctaact gaccataaga taactatgtt gctcctttta 9420
cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt 9480
tcattttctc ctccttgtat attgatacaa cgaggaaaat gcgatacacc tatgcgacga 9540
aattacggaa acatagtacg ataacgaagg gcataccgaa agtaaaagag gaggaacata 9600
aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 9660
gtgtgcactg tgtttgctga cgcaaccccc actggttggg tttaggacca acgacagaga 9720
aatactcctc aacaccgggc aacagtccgt tgcaccgcac cacacgtgac acaaacgact 9780
gcgttggggg tgaccaaccc gcattgccac cacctgtcag ctcctttccg ggactttcgc 9840
tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 9900
cgtaacggtg gtggacagtc gaggaaaggc cctgaaagcg aaagggggag ggataacggt 9960
gccgccttga gtagcggcgg acggaacggg cgacgacctg aggggctcgg ctgttgggca 10020
ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg 10080
ttgccacctg gattctgcgc tccccgagcc gacaacccgt gactgttaag gcaccacaac 10140
agccccttta gtagcaggaa aggaaccgac gagcggacac aacggtggac ctaagacgcg 10200
gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 10260
ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc ccctgcagga agacgatgca 10320
gggaagccgg gagttaggtc gcctggaagg aagggcgccg gacgacggcc gagacgccgg 10380
agaaggcgca gaagcggaag gccctcagac gagtcggatc tccctttggg ccgcctcccc 10440
gcatcgatac cgtcgactag ccgtaccttt aagaccaatg acttacaagg cagctgtaga 10500
cgggagtctg ctcagcctag agggaaaccc ggcggagggg cgtagctatg gcagctgatc 10560
ggcatggaaa ttctggttac tgaatgttcc gtcgacatct tcttagccac tttttaaaag 10620
aaaagggggg actggaaggg ctaattcact cccaaagaag acaagatctg ctttttgcct 10680
gtactgggtc tctctggtta agaatcggtg aaaaattttc ttttcccccc tgaccttccc 10740
gattaagtga gggtttcttc tgttctagac gaaaaacgga catgacccag agagaccaat 10800
gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa 10860
taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc ctggtctaga ctcggaccct 10920
cgagagaccg attgatccct tgggtgacga attcggagtt atttcgaacg gaactcacga 10980
agttcatcac acacgggcag tgttgtgtga ctctggtaac tagagatccc tcagaccctt 11040
ttagtcagtg tggaaaatct ctagcagaat tcgatatcaa gcttatcgat accgtcgacc 11100
acaacacact gagaccattg atctctaggg agtctgggaa aatcagtcac accttttaga 11160
gatcgtctta agctatagtt cgaatagcta tggcagctgg tcgagggggg gcccggtacc 11220
caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga 11280
ctgggaaaac cctggcgtta agctcccccc cgggccatgg gttaagcggg atatcactca 11340
gcataatgtt aagtgaccgg cagcaaaatg ttgcagcact gacccttttg ggaccgcaat 11400
cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg 11460
cccgcaccga tcgcccttcc caacagttgc gcagcctgaa gggttgaatt agcggaacgt 11520
cgtgtagggg gaaagcggtc gaccgcatta tcgcttctcc gggcgtggct agcgggaagg 11580
gttgtcaacg cgtcggactt tggcgaatgg aaattgtaag cgttaatatt ttgttaaaat 11640
tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa 11700
accgcttacc tttaacattc gcaattataa aacaatttta agcgcaattt aaaaacaatt 11760
tagtcgagta aaaaattggt tatccggctt tagccgtttt tcccttataa atcaaaagaa 11820
tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac 11880
gtggactcca acgtcaaagg agggaatatt tagttttctt atctggctct atcccaactc 11940
acaacaaggt caaaccttgt tctcaggtga taatttcttg cacctgaggt tgcagtttcc 12000
gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt 12060
tttggggtcg aggtgccgta aagcactaaa tcggaaccct cgctttttgg cagatagtcc 12120
cgctaccggg tgatgcactt ggtagtggga ttagttcaaa aaaccccagc tccacggcat 12180
ttcgtgattt agccttggga aaagggagcc cccgatttag agcttgacgg ggaaagccgg 12240
cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa 12300
tttccctcgg gggctaaatc tcgaactgcc cctttcggcc gcttgcaccg ctctttcctt 12360
cccttctttc gctttcctcg cccgcgatcc cgcgaccgtt gtgtagcggt cacgctgcgc 12420
gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt 12480
cggggaaatg tgcgcggaac cacatcgcca gtgcgacgcg cattggtggt gtgggcggcg 12540
cgaattacgc ggcgatgtcc cgcgcagtcc accgtgaaaa gcccctttac acgcgccttg 12600
ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 12660
ctgataaatg cttcaataat attgaaaaag gaagagtatg gggataaaca aataaaaaga 12720
tttatgtaag tttatacata ggcgagtact ctgttattgg gactatttac gaagttatta 12780
taactttttc cttctcatac agtattcaac atttccgtgt cgcccttatt cccttttttg 12840
cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg 12900
tcataagttg taaaggcaca gcgggaataa gggaaaaaac gccgtaaaac ggaaggacaa 12960
aaacgagtgg gtctttgcga ccactttcat tttctacgac aagatcagtt gggtgcacga 13020
gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa 13080
gaacgttttc caatgatgag ttctagtcaa cccacgtgct cacccaatgt agcttgacct 13140
agagttgtcg ccattctagg aactctcaaa agcggggctt cttgcaaaag gttactactc 13200
cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca 13260
actcggtcgc cgcatacact attctcagaa tgacttggtt gtgaaaattt caagacgata 13320
caccgcgcca taatagggca taactgcggc ccgttctcgt tgagccagcg gcgtatgtga 13380
taagagtctt actgaaccaa gagtactcac cagtcacaga aaagcatctt acggatggca 13440
tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact 13500
ctcatgagtg gtcagtgtct tttcgtagaa tgcctaccgt actgtcattc tcttaatacg 13560
tcacgacggt attggtactc actattgtga cgccggttga tacttctgac aacgatcgga 13620
ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 13680
cgttgggaac cggagctgaa atgaagactg ttgctagcct cctggcttcc tcgattggcg 13740
aaaaaacgtg ttgtaccccc tagtacattg agcggaacta gcaacccttg gcctcgactt 13800
tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt 13860
gcgcaaacta ttaactggcg aactacttac tctagcttcc acttcggtat ggtttgctgc 13920
tcgcactgtg gtgctacgga catcgttacc gttgttgcaa cgcgtttgat aattgaccgc 13980
ttgatgaatg agatcgaagg cggcaacaat taatagactg gatggaggcg gataaagttg 14040
caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag 14100
gccgttgtta attatctgac ctacctccgc ctatttcaac gtcctggtga agacgcgagc 14160
cgggaaggcc gaccgaccaa ataacgacta tttagacctc ccggtgagcg tgggtctcgc 14220
ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 14280
acggggagtc aggcaactat ggccactcgc acccagagcg ccatagtaac gtcgtgaccc 14340
cggtctacca ttcgggaggg catagcatca atagatgtgc tgcccctcag tccgttgata 14400
ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 14460
gtcagaccaa gtttactcat atatacttta gattgattta cctacttgct ttatctgtct 14520
agcgactcta tccacggagt gactaattcg taaccattga cagtctggtt caaatgagta 14580
tatatgaaat ctaactaaat aaacttcatt tttaatttaa aaggatctag gtgaagatcc 14640
tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 14700
tttgaagtaa aaattaaatt ttcctagatc cacttctagg aaaaactatt agagtactgg 14760
ttttagggaa ttgcactcaa aagcaaggtg actcgcagtc accccgtaga aaagatcaaa 14820
ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 14880
ccgctaccag cggtggtttg tggggcatct tttctagttt cctagaagaa ctctaggaaa 14940
aaaagacgcg cattagacga cgaacgtttg tttttttggt ggcgatggtc gccaccaaac 15000
tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 15060
gataccaaat actgttcttc tagtgtagcc gtagttaggc aaacggccta gttctcgatg 15120
gttgagaaaa aggcttccat tgaccgaagt cgtctcgcgt ctatggttta tgacaagaag 15180
atcacatcgg catcaatccg caccacttca agaactctgt agcaccgcct acatacctcg 15240
ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 15300
gtggtgaagt tcttgagaca tcgtggcgga tgtatggagc gagacgatta ggacaatggt 15360
caccgacgac ggtcaccgct attcagcaca gaatggccca tggactcaag acgatagtta 15420
ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 15480
cgaacgacct acaccgaact acctgagttc tgctatcaat ggcctattcc gcgtcgccag 15540
cccgacttgc cccccaagca cgtgtgtcgg gtcgaacctc gcttgctgga tgtggcttga 15600
gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 15660
caggtatccg gtaagcggca gggtcggaac aggagagcgc ctctatggat gtcgcactcg 15720
atactctttc gcggtgcgaa gggcttccct ctttccgcct gtccataggc cattcgccgt 15780
cccagccttg tcctctcgcg acgagggagc ttccaggggg aaacgcctgg tatctttata 15840
gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 15900
tgctccctcg aaggtccccc tttgcggacc atagaaatat caggacagcc caaagcggtg 15960
gagactgaac tcgcagctaa aaacactacg agcagtcccc ggcggagcct atggaaaaac 16020
gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc 16080
tttcctgcgt tatcccctga ccgcctcgga tacctttttg cggtcgttgc gccggaaaaa 16140
tgccaaggac cggaaaacga ccggaaaacg agtgtacaag aaaggacgca ataggggact 16200
ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac 16260
gaccgagcgc agcgagtcag tgagcgagga agcggaagag aagacaccta ttggcataat 16320
ggcggaaact cactcgacta tggcgagcgg cgtcggcttg ctggctcgcg tcgctcagtc 16380
actcgctcct tcgccttctc cgcccaatac gcaaaccgcc tctccccgcg cgttggccga 16440
ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg 16500
gcgggttatg cgtttggcgg agaggggcgc gcaaccggct aagtaattac gtcgaccgtg 16560
ctgtccaaag ggctgacctt tcgcccgtca ctcgcgttgc caattaatgt gagttagctc 16620
actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt 16680
gtgagcggat aacaatttca gttaattaca ctcaatcgag tgagtaatcc gtggggtccg 16740
aaatgtgaaa tacgaaggcc gagcatacaa cacaccttaa cactcgccta ttgttaaagt 16800
cacaggaaac agctatgacc atgattacgc caagctcgaa attaaccctc actaaaggga 16860
acaaaagctg gagctccacc gcggtggcgg cctcgaggtc gtgtcctttg tcgatactgg 16920
tactaatgcg gttcgagctt taattgggag tgatttccct tgttttcgac ctcgaggtgg 16980
cgccaccgcc ggagctccag gagatccggt cgaccagcaa ccatagtccc gcccctaact 17040
ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 17100
ctctaggcca gctggtcgtt ggtatcaggg cggggattga ggcgggtagg gcggggattg 17160
aggcgggtca aggcgggtaa gaggcggggt accgactgat atttttttta tttatgcaga 17220
ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 17280
cctaggcttt tgcaaaaagc taaaaaaaat aaatacgtct ccggctccgg cggagccgga 17340
gactcgataa ggtcttcatc actcctccga aaaaacctcc ggatccgaaa acgtttttcg 17400
ttcgacggta tcgattggct catgtccaac attaccgcca tgttgacatt gattattgac 17460
tagttattaa tagtaatcaa ttacggggtc attagttcat aagctgccat agctaaccga 17520
gtacaggttg taatggcggt acaactgtaa ctaataactg atcaataatt atcattagtt 17580
aatgccccag taatcaagta agcccatata tggagttccg cgttacataa cttacggtaa 17640
atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 17700
tcgggtatat acctcaaggc gcaatgtatt gaatgccatt taccgggcgg accgactggc 17760
gggttgctgg gggcgggtaa ctgcagttat tactgcatac ttcccatagt aacgccaata 17820
gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta 17880
catcaagtgt atcatatgcc aagggtatca ttgcggttat ccctgaaagg taactgcagt 17940
tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca tagtatacgg 18000
aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 18060
catgacctta tgggactttc ctacttggca gtacatctac ttcatgcggg ggataactgc 18120
agttactgcc atttaccggg cggaccgtaa tacgggtcat gtactggaat accctgaaag 18180
gatgaaccgt catgtagatg gtattagtca tcgctattac catggtgatg cggttttggc 18240
agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 18300
cataatcagt agcgataatg gtaccactac gccaaaaccg tcatgtagtt acccgcacct 18360
atcgccaaac tgagtgcccc taaaggttca gaggtggggt ttgacgtcaa tgggagtttg 18420
ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 18480
caaatgggcg gtaggcgtgt aactgcagtt accctcaaac aaaaccgtgg ttttagttgc 18540
cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc catccgcaca 18600
acggaattcg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta 18660
ctgggtctct ctgtgcctta agcctcaccg ctcgggagtc taggacgtat attcgtcgac 18720
gaaaaacgga catgacccag agagac                                      18746

<210> 57
<211> 18992
<212> DNA
<213> Artificial Sequence

<220> 
<223> Ce7scFv-IgG4hinge-CD28tm/cyto-4-1BB-zeta-T2A-EGFRt
      -epHIV7 (short two costimulatory domains)

<400> 57
gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc 60
tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc caatctggtc tagactcgga 120
ccctcgagag accgattgat cccttgggtg acgaattcgg agttatttcg aacggaactc 180
acgaagttca tcacacacgg cgtctgttgt gtgactctgg taactagaga tccctcagac 240
ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa 300
gcagacaaca cactgagacc attgatctct agggagtctg ggaaaatcag tcacaccttt 360
tagagatcgt caccgcgggc ttgtccctga actttcgctt agggaaacca gaggagctct 420
ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg 480
tgagtacgcc aaaaattttg tccctttggt ctcctcgaga gagctgcgtc ctgagccgaa 540
cgacttcgcg cgtgccgttc tccgctcccc gccgctgacc actcatgcgg tttttaaaac 600
actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 660
attagatcga tgggaaaaaa ttcggttaag gccaggggga tgatcgcctc cgatcttcct 720
ctctctaccc acgctctcgc agtcataatt cgccccctct taatctagct accctttttt 780
aagccaattc cggtccccct aagaaaaaat ataaattaaa acatatagta tgggcaagca 840
gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac 900
ttctttttta tatttaattt tgtatatcat acccgttcgt ccctcgatct tgctaagcgt 960
caattaggac cggacaatct ttgtagtctt ccgacatctg aaatactggg acagctacaa 1020
ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc 1080
tattgtgtgc atcaaaggat tttatgaccc tgtcgatgtt ggtagggaag tctgtcctag 1140
tcttcttgaa tctagtaata tattatgtca tcgttgggag ataacacacg tagtttccta 1200
agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa 1260
gaaaaaagca cagcaagcag cagctgacac aggacacagc tctctatttt ctgtggttcc 1320
ttcgaaatct gttctatctc cttctcgttt tgttttcatt cttttttcgt gtcgttcgtc 1380
gtcgactgtg tcctgtgtcg aatcaggtca gccaaaatta ccctatagtg cagaacatcc 1440
aggggcaaat ggtacatcag gccatatcac ctagaacttt aaatgcatgg gtaaaagtag 1500
ttagtccagt cggttttaat gggatatcac gtcttgtagg tccccgttta ccatgtagtc 1560
cggtatagtg gatcttgaaa tttacgtacc cattttcatc tagaagagaa ggctttcagc 1620
ccagaagtga tacccatgtt ttcagcatta tcagaaggag ccaccccaca agatttaaac 1680
accatgctaa acacagtggg atcttctctt ccgaaagtcg ggtcttcact atgggtacaa 1740
aagtcgtaat agtcttcctc ggtggggtgt tctaaatttg tggtacgatt tgtgtcaccc 1800
gggacatcaa gcagccatgc aaatgttaaa agagaccatc aatgaggaag ctgcaggcaa 1860
agagaagagt ggtgcagaga gaaaaaagag cagtgggaat ccctgtagtt cgtcggtacg 1920
tttacaattt tctctggtag ttactccttc gacgtccgtt tctcttctca ccacgtctct 1980
cttttttctc gtcaccctta aggagctttg ttccttgggt tcttgggagc agcaggaagc 2040
actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata 2100
tcctcgaaac aaggaaccca agaaccctcg tcgtccttcg tgatacccgc gtcgcagtta 2160
ctgcgactgc catgtccggt ctgttaataa cagaccatat gtgcagcagc agaacaattt 2220
gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 2280
gctccaggca agaatcctgg cacgtcgtcg tcttgttaaa cgactcccga taactccgcg 2340
ttgtcgtaga caacgttgag tgtcagaccc cgtagttcgt cgaggtccgt tcttaggacc 2400
ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac 2460
tcatttgcac cactgctgtg ccttggatct acaaatggca gacacctttc tatggatttc 2520
ctagttgtcg aggaccccta aaccccaacg agaccttttg agtaaacgtg gtgacgacac 2580
ggaacctaga tgtttaccgt gtattcatcc acaattttaa aagaaaaggg gggattgggg 2640
ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat 2700
cataagtagg tgttaaaatt ttcttttccc ccctaacccc ccatgtcacg tcccctttct 2760
tatcatctgt attatcgttg tctgtatgtt tgatttctta tacaaaaaca aattacaaaa 2820
attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttgggg atcaattgca 2880
tgaagaatct gcttagggtt atgtttttgt ttaatgtttt taagttttaa aagcccaaat 2940
aatgtccctg tcgtctctag gtcaaacccc tagttaacgt acttcttaga cgaatcccaa 3000
aggcgttttg cgctgcttcg cgaggatctg cgatcgctcc ggtgcccgtc agtgggcaga 3060
gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg tccgcaaaac gcgacgaagc 3120
gctcctagac gctagcgagg ccacgggcag tcacccgtct cgcgtgtagc gggtgtcagg 3180
ggctcttcaa cccccctccc gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg 3240
taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac 3300
cagccgttaa cttggccacg gatctcttcc accgcgcccc atttgaccct ttcactacag 3360
cacatgaccg aggcggaaaa agggctccca ccccctcttg cgtatataag tgcagtagtc 3420
gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg 3480
gctcgcatct ctccttcacg gcatatattc acgtcatcag cggcacttgc aagaaaaagc 3540
gttgcccaaa cggcggtctt gtgtcgactt cgaagctccc cgagcgtaga gaggaagtgc 3600
cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc 3660
cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta gcgggcggcg ggatggactc 3720
cggcggtagg tgcggccaac tcagcgcaag acggcggagg gcggacacca cggaggactt 3780
gacgcaggcg gcagatccat agtttaaagc tcaggtcgag accgggcctt tgtccggcgc 3840
tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc 3900
tcaaatttcg agtccagctc tggcccggaa acaggccgcg agggaacctc ggatggatct 3960
gagtcggccg agaggtgcga aacggactgg gacgaacgag aactctacgt ctttgtttcg 4020
ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacggc tagcgccgcc 4080
accatgctgc tgctggtgac ttgagatgca gaaacaaagc aaaagacaag acgcggcaat 4140
gtctaggttc gacactggcc gcggatgccg atcgcggcgg tggtacgacg acgaccactg 4200
cagcctgctg ctgtgcgagc tgccccaccc cgcctttctg ctgatccccc aggtgcagct 4260
gcagcagcct ggcgccgagc tggtgaagcc aggcgccagc gtcggacgac gacacgctcg 4320
acggggtggg gcggaaagac gactaggggg tccacgtcga cgtcgtcgga ccgcggctcg 4380
accacttcgg tccgcggtcg gtgaagctgt cctgcaaggc cagcggctac accttcaccg 4440
gctactggat gcactgggtg aagcagagac ccggccacgg cctggaatgg atcggcgaga 4500
cacttcgaca ggacgttccg gtcgccgatg tggaagtggc cgatgaccta cgtgacccac 4560
ttcgtctctg ggccggtgcc ggaccttacc tagccgctct tcaaccccag caacggccgg 4620
accaactaca acgagcggtt caagagcaag gccaccctga ccgtggacaa gagcagcacc 4680
accgccttca tgcagctgtc agttggggtc gttgccggcc tggttgatgt tgctcgccaa 4740
gttctcgttc cggtgggact ggcacctgtt ctcgtcgtgg tggcggaagt acgtcgacag 4800
cggcctgacc agcgaggaca gcgccgtgta cttctgcgcc agggactact acggcaccag 4860
ctacaacttc gactactggg gccagggcac cacactgacc gccggactgg tcgctcctgt 4920
cgcggcacat gaagacgcgg tccctgatga tgccgtggtc gatgttgaag ctgatgaccc 4980
cggtcccgtg gtgtgactgg gtgagcagcg gcggaggggg ctctggcggc ggaggatctg 5040
ggggaggggg cagcgacatc cagatgaccc agagcagcag cagcttcagc gtgagcctgg 5100
cactcgtcgc cgcctccccc gagaccgccg cctcctagac cccctccccc gtcgctgtag 5160
gtctactggg tctcgtcgtc gtcgaagtcg cactcggacc gcgaccgggt gaccatcacc 5220
tgtaaggcca acgaggacat caacaaccgg ctggcctggt atcagcagac ccccggcaac 5280
agccccaggc tgctgatcag cgctggccca ctggtagtgg acattccggt tgctcctgta 5340
gttgttggcc gaccggacca tagtcgtctg ggggccgttg tcggggtccg acgactagtc 5400
cggcgccacc aacctggtga ccggcgtgcc cagccggttt agcggcagcg gctccggcaa 5460
ggactacacc ctgaccatca caagcctgca ggccgaggac gccgcggtgg ttggaccact 5520
ggccgcacgg gtcggccaaa tcgccgtcgc cgaggccgtt cctgatgtgg gactggtagt 5580
gttcggacgt ccggctcctg ttcgccacct actactgcca gcagtactgg tccaccccct 5640
tcaccttcgg cagcggcacc gagctggaaa tcaaagaatc taagtacgga ccgccctgcc 5700
aagcggtgga tgatgacggt cgtcatgacc aggtggggga agtggaagcc gtcgccgtgg 5760
ctcgaccttt agtttcttag attcatgcct ggcgggacgg ccccttgccc tatgttctgg 5820
gtgctggtgg tggtcggagg cgtgctggcc tgctacagcc tgctggtcac cgtggccttc 5880
atcatctttt gggtccgcag ggggaacggg atacaagacc cacgaccacc accagcctcc 5940
gcacgaccgg acgatgtcgg acgaccagtg gcaccggaag tagtagaaaa cccaggcgtc 6000
caagcggagc agaggcggcc acagcgacta catgaacatg acccctagac ggcctggccc 6060
caccagaaag cactaccagc cctacgcccc tccccgggac gttcgcctcg tctccgccgg 6120
tgtcgctgat gtacttgtac tggggatctg ccggaccggg gtggtctttc gtgatggtcg 6180
ggatgcgggg aggggccctg tttgccgcct acagaagcaa acggggcaga aagaaactcc 6240
tgtatatatt caaacaacca tttatgagac cagtacaaac tactcaagag gaagatggct 6300
aaacggcgga tgtcttcgtt tgccccgtct ttctttgagg acatatataa gtttgttggt 6360
aaatactctg gtcatgtttg atgagttctc cttctaccga gtagctgccg atttccagaa 6420
gaagaagaag gaggatgtga actgcgggtg aagttcagca gaagcgccga cgcccctgcc 6480
taccagcagg gccagaatca catcgacggc taaaggtctt cttcttcttc ctcctacact 6540
tgacgcccac ttcaagtcgt cttcgcggct gcggggacgg atggtcgtcc cggtcttagt 6600
gctgtacaac gagctgaacc tgggcagaag ggaagagtac gacgtcctgg ataagcggag 6660
aggccgggac cctgagatgg gcggcaagcc tcggcggaag cgacatgttg ctcgacttgg 6720
acccgtcttc ccttctcatg ctgcaggacc tattcgcctc tccggccctg ggactctacc 6780
cgccgttcgg agccgccttc aacccccagg aaggcctgta taacgaactg cagaaagaca 6840
agatggccga ggcctacagc gagatcggca tgaagggcga gcggaggcgg ggcaagggcc 6900
ttgggggtcc ttccggacat attgcttgac gtctttctgt tctaccggct ccggatgtcg 6960
ctctagccgt acttcccgct cgcctccgcc ccgttcccgg acgacggcct gtatcagggc 7020
ctgtccaccg ccaccaagga tacctacgac gccctgcaca tgcaggccct gcccccaagg 7080
ctcgagggcg gcggagaggg tgctgccgga catagtcccg gacaggtggc ggtggttcct 7140
atggatgctg cgggacgtgt acgtccggga cgggggttcc gagctcccgc cgcctctccc 7200
cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta ggatgcttct 7260
cctggtgaca agccttctgc tctgtgagtt accacaccca gtctccttca gaagattgta 7320
cgccactgca cctcctctta gggccgggat cctacgaaga ggaccactgt tcggaagacg 7380
agacactcaa tggtgtgggt gcattcctcc tgatcccacg caaagtgtgt aacggaatag 7440
gtattggtga atttaaagac tcactctcca taaatgctac gaatattaaa cacttcaaaa 7500
cgtaaggagg actagggtgc gtttcacaca ttgccttatc cataaccact taaatttctg 7560
agtgagaggt atttacgatg cttataattt gtgaagtttt actgcacctc catcagtggc 7620
gatctccaca tcctgccggt ggcatttagg ggtgactcct tcacacatac tcctcctctg 7680
gatccacagg aactggatat tgacgtggag gtagtcaccg ctagaggtgt aggacggcca 7740
ccgtaaatcc ccactgagga agtgtgtatg aggaggagac ctaggtgtcc ttgacctata 7800
tctgaaaacc gtaaaggaaa tcacagggtt tttgctgatt caggcttggc ctgaaaacag 7860
gacggacctc catgcctttg agaacctaga aatcatacgc agacttttgg catttccttt 7920
agtgtcccaa aaacgactaa gtccgaaccg gacttttgtc ctgcctggag gtacggaaac 7980
tcttggatct ttagtatgcg ggcaggacca agcaacatgg tcagttttct cttgcagtcg 8040
tcagcctgaa cataacatcc ttgggattac gctccctcaa ggagataagt gatggagatg 8100
ccgtcctggt tcgttgtacc agtcaaaaga gaacgtcagc agtcggactt gtattgtagg 8160
aaccctaatg cgagggagtt cctctattca ctacctctac tgataatttc aggaaacaaa 8220
aatttgtgct atgcaaatac aataaactgg aaaaaactgt ttgggacctc cggtcagaaa 8280
accaaaatta taagcaacag actattaaag tcctttgttt ttaaacacga tacgtttatg 8340
ttatttgacc ttttttgaca aaccctggag gccagtcttt tggttttaat attcgttgtc 8400
aggtgaaaac agctgcaagg ccacaggcca ggtctgccat gccttgtgct cccccgaggg 8460
ctgctggggc ccggagccca gggactgcgt ctcttgccgg tccacttttg tcgacgttcc 8520
ggtgtccggt ccagacggta cggaacacga gggggctccc gacgaccccg ggcctcgggt 8580
ccctgacgca gagaacggcc aatgtcagcc gaggcaggga atgcgtggac aagtgcaacc 8640
ttctggaggg tgagccaagg gagtttgtgg agaactctga gtgcatacag tgccacccag 8700
ttacagtcgg ctccgtccct tacgcacctg ttcacgttgg aagacctccc actcggttcc 8760
ctcaaacacc tcttgagact cacgtatgtc acggtgggtc agtgcctgcc tcaggccatg 8820
aacatcacct gcacaggacg gggaccagac aactgtatcc agtgtgccca ctacattgac 8880
ggcccccact gcgtcaagac tcacggacgg agtccggtac ttgtagtgga cgtgtcctgc 8940
ccctggtctg ttgacatagg tcacacgggt gatgtaactg ccgggggtga cgcagttctg 9000
ctgcccggca ggagtcatgg gagaaaacaa caccctggtc tggaagtacg cagacgccgg 9060
ccatgtgtgc cacctgtgcc atccaaactg cacctacgga gacgggccgt cctcagtacc 9120
ctcttttgtt gtgggaccag accttcatgc gtctgcggcc ggtacacacg gtggacacgg 9180
taggtttgac gtggatgcct tgcactgggc caggtcttga aggctgtcca acgaatgggc 9240
ctaagatccc gtccatcgcc actgggatgg tgggggccct cctcttgctg ctggtggtgg 9300
acgtgacccg gtccagaact tccgacaggt tgcttacccg gattctaggg caggtagcgg 9360
tgaccctacc acccccggga ggagaacgac gaccaccacc ccctggggat cggcctcttc 9420
atgtgagcgg ccgctctaga cccgggctgc aggaattcga tatcaagctt atcgataatc 9480
aacctctgga ttacaaaatt gggaccccta gccggagaag tacactcgcc ggcgagatct 9540
gggcccgacg tccttaagct atagttcgaa tagctattag ttggagacct aatgttttaa 9600
tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct 9660
gctttaatgc ctttgtatca tgctattgct tcccgtatgg acactttcta actgaccata 9720
agaattgata caacgaggaa aatgcgatac acctatgcga cgaaattacg gaaacatagt 9780
acgataacga agggcatacc ctttcatttt ctcctccttg tataaatcct ggttgctgtc 9840
tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc 9900
gaaagtaaaa gaggaggaac atatttagga ccaacgacag agaaatactc ctcaacaccg 9960
ggcaacagtc cgttgcaccg caccacacgt gacacaaacg tgacgcaacc cccactggtt 10020
ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 10080
ccacggcgga actcatcgcc actgcgttgg gggtgaccaa ccccgtaacg gtggtggaca 10140
gtcgaggaaa ggccctgaaa gcgaaagggg gagggataac ggtgccgcct tgagtagcgg 10200
gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg 10260
ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct cggacggaac gggcgacgac 10320
ctgtccccga gccgacaacc cgtgactgtt aaggcaccac aacagcccct ttagtagcag 10380
gaaaggaacc gacgagcgga gtgttgccac ctggattctg cgcgggacgt ccttctgcta 10440
cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg 10500
cacaacggtg gacctaagac gcgccctgca ggaagacgat gcagggaagc cgggagttag 10560
gtcgcctgga aggaagggcg ccggacgacg gccgagacgc gcctcttccg cgtcttcgcc 10620
ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac 10680
tagccgtacc tttaagacca cggagaaggc gcagaagcgg aagcgggagt ctgctcagcc 10740
tagagggaaa cccggcggag gggcgtagct atggcagctg atcggcatgg aaattctggt 10800
atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa 10860
gggctaattc actcccaaag aagacaagat ctgctttttg tactgaatgt tccgtcgaca 10920
tctagaatcg gtgaaaaatt ttcttttccc ccctgacctt cccgattaag tgagggtttc 10980
ttctgttcta gacgaaaaac cctgtactgg gtctctctgg ttagaccaga tctgagcctg 11040
ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 11100
ggacatgacc cagagagacc aatctggtct agactcggac cctcgagaga ccgattgatc 11160
ccttgggtga cgaattcgga gttatttcga acggaactca gcttcaagta gtgtgtgccc 11220
gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 11280
tctctagcag aattcgatat cgaagttcat cacacacggg cagacaacac actgagacca 11340
ttgatctcta gggagtctgg gaaaatcagt cacacctttt agagatcgtc ttaagctata 11400
caagcttatc gataccgtcg acctcgaggg ggggcccggt acccaattcg ccctatagtg 11460
agtcgtatta caattcactg gccgtcgttt tacaacgtcg gttcgaatag ctatggcagc 11520
tggagctccc ccccgggcca tgggttaagc gggatatcac tcagcataat gttaagtgac 11580
cggcagcaaa atgttgcagc tgactgggaa aaccctggcg ttacccaact taatcgcctt 11640
gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct 11700
actgaccctt ttgggaccgc aatgggttga attagcggaa cgtcgtgtag ggggaaagcg 11760
gtcgaccgca ttatcgcttc tccgggcgtg gctagcggga tcccaacagt tgcgcagcct 11820
gaatggcgaa tggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt 11880
taaatcagct cattttttaa agggttgtca acgcgtcgga cttaccgctt acctttaaca 11940
ttcgcaatta taaaacaatt ttaagcgcaa tttaaaaaca atttagtcga gtaaaaaatt 12000
ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt 12060
gagtgttgtt ccagtttgga acaagagtcc actattaaag ggttatccgg ctttagccgt 12120
tttagggaat atttagtttt cttatctggc tctatcccaa ctcacaacaa ggtcaaacct 12180
tgttctcagg tgataatttc aacgtggact ccaacgtcaa agggcgaaaa accgtctatc 12240
agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc 12300
ttgcacctga ggttgcagtt tcccgctttt tggcagatag tcccgctacc gggtgatgca 12360
cttggtagtg ggattagttc aaaaaacccc agctccacgg gtaaagcact aaatcggaac 12420
cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 12480
gaagggaaga aagcgaaagg catttcgtga tttagccttg ggatttccct cgggggctaa 12540
atctcgaact gcccctttcg gccgcttgca ccgctctttc cttcccttct ttcgctttcc 12600
agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc 12660
cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tcgcccgcga tcccgcgacc 12720
gttcacatcg ccagtgcgac gcgcattggt ggtgtgggcg gcgcgaatta cgcggcgatg 12780
tcccgcgcag tccaccgtga tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 12840
tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 12900
aaagcccctt tacacgcgcc ttggggataa acaaataaaa agatttatgt aagtttatac 12960
ataggcgagt actctgttat tgggactatt tacgaagtta aatattgaaa aaggaagagt 13020
atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct 13080
gtttttgctc acccagaaac ttataacttt ttccttctca tactcataag ttgtaaaggc 13140
acagcgggaa taagggaaaa aacgccgtaa aacggaagga caaaaacgag tgggtctttg 13200
gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact 13260
ggatctcaac agcggtaaga tccttgagag ttttcgcccc cgaccacttt cattttctac 13320
gacttctagt caacccacgt gctcacccaa tgtagcttga cctagagttg tcgccattct 13380
aggaactctc aaaagcgggg gaagaacgtt ttccaatgat gagcactttt aaagttctgc 13440
tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 13500
cttcttgcaa aaggttacta ctcgtgaaaa tttcaagacg atacaccgcg ccataatagg 13560
gcataactgc ggcccgttct cgttgagcca gcggcgtatg actattctca gaatgacttg 13620
gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta 13680
tgcagtgctg ccataaccat tgataagagt cttactgaac caactcatga gtggtcagtg 13740
tcttttcgta gaatgcctac cgtactgtca ttctcttaat acgtcacgac ggtattggta 13800
gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac 13860
cgcttttttg cacaacatgg gggatcatgt aactcgcctt ctcactattg tgacgccggt 13920
tgaatgaaga ctgttgctag cctcctggct tcctcgattg gcgaaaaaac gtgttgtacc 13980
ccctagtaca ttgagcggaa gatcgttggg aaccggagct gaatgaagcc ataccaaacg 14040
acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 14100
ctagcaaccc ttggcctcga cttacttcgg tatggtttgc tgctcgcact gtggtgctac 14160
ggacatcgtt accgttgttg caacgcgttt gataattgac gcgaactact tactctagct 14220
tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc 14280
tcggcccttc cggctggctg cgcttgatga atgagatcga agggccgttg ttaattatct 14340
gacctacctc cgcctatttc aacgtcctgg tgaagacgcg agccgggaag gccgaccgac 14400
gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact 14460
ggggccagat ggtaagccct cccgtatcgt agttatctac caaataacga ctatttagac 14520
ctcggccact cgcacccaga gcgccatagt aacgtcgtga ccccggtcta ccattcggga 14580
gggcatagca tcaatagatg acgacgggga gtcaggcaac tatggatgaa cgaaatagac 14640
agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 14700
tgctgcccct cagtccgttg atacctactt gctttatctg tctagcgact ctatccacgg 14760
agtgactaat tcgtaaccat tgacagtctg gttcaaatga catatatact ttagattgat 14820
ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 14880
accaaaatcc cttaacgtga gtatatatga aatctaacta aattttgaag taaaaattaa 14940
attttcctag atccacttct aggaaaaact attagagtac tggttttagg gaattgcact 15000
gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 15060
tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa caaaagcaag gtgactcgca 15120
gtctggggca tcttttctag tttcctagaa gaactctagg aaaaaaagac gcgcattaga 15180
cgacgaacgt ttgttttttt ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 15240
taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc 15300
ggtggcgatg gtcgccacca aacaaacggc ctagttctcg atggttgaga aaaaggcttc 15360
cattgaccga agtcgtctcg cgtctatggt ttatgacaag ttctagtgta gccgtagtta 15420
ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 15480
ccagtggctg ctgccagtgg aagatcacat cggcatcaat ccggtggtga agttcttgag 15540
acatcgtggc ggatgtatgg agcgagacga ttaggacaat ggtcaccgac gacggtcacc 15600
cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 15660
gtcgggctga acggggggtt cgtgcacaca gcccagcttg gctattcagc acagaatggc 15720
ccaacctgag ttctgctatc aatggcctat tccgcgtcgc cagcccgact tgccccccaa 15780
gcacgtgtgt cgggtcgaac gagcgaacga cctacaccga actgagatac ctacagcgtg 15840
agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 15900
ctcgcttgct ggatgtggct tgactctatg gatgtcgcac tcgatactct ttcgcggtgc 15960
gaagggcttc cctctttccg cctgtccata ggccattcgc gcagggtcgg aacaggagag 16020
cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 16080
cacctctgac ttgagcgtcg cgtcccagcc ttgtcctctc gcgtgctccc tcgaaggtcc 16140
ccctttgcgg accatagaaa tatcaggaca gcccaaagcg gtggagactg aactcgcagc 16200
atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 16260
tttacggttc ctggcctttt gctggccttt tgctcacatg taaaaacact acgagcagtc 16320
cccccgcctc ggataccttt ttgcggtcgt tgcgccggaa aaatgccaag gaccggaaaa 16380
cgaccggaaa acgagtgtac ttctttcctg cgttatcccc tgattctgtg gataaccgta 16440
ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 16500
aagaaaggac gcaatagggg actaagacac ctattggcat aatggcggaa actcactcga 16560
ctatggcgag cggcgtcggc ttgctggctc gcgtcgctca cagtgagcga ggaagcggaa 16620
gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg 16680
cacgacaggt ttcccgactg gtcactcgct ccttcgcctt ctcgcgggtt atgcgtttgg 16740
cggagagggg cgcgcaaccg gctaagtaat tacgtcgacc gtgctgtcca aagggctgac 16800
gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca 16860
ggctttacac tttatgcttc cggctcgtat gttgtgtgga ctttcgcccg tcactcgcgt 16920
tgcgttaatt acactcaatc gagtgagtaa tccgtggggt ccgaaatgtg aaatacgaag 16980
gccgagcata caacacacct attgtgagcg gataacaatt tcacacagga aacagctatg 17040
accatgatta cgccaagctc gaaattaacc ctcactaaag ggaacaaaag ctggagctcc 17100
taacactcgc ctattgttaa agtgtgtcct ttgtcgatac tggtactaat gcggttcgag 17160
ctttaattgg gagtgatttc ccttgttttc gacctcgagg accgcggtgg cggcctcgag 17220
gtcgagatcc ggtcgaccag caaccatagt cccgccccta actccgccca tcccgcccct 17280
aactccgccc agttccgccc tggcgccacc gccggagctc cagctctagg ccagctggtc 17340
gttggtatca gggcggggat tgaggcgggt agggcgggga ttgaggcggg tcaaggcggg 17400
attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 17460
cctctgagct attccagaag tagtgaggag gcttttttgg taagaggcgg ggtaccgact 17520
gattaaaaaa aataaatacg tctccggctc cggcggagcc ggagactcga taaggtcttc 17580
atcactcctc cgaaaaaacc aggcctaggc ttttgcaaaa agcttcgacg gtatcgattg 17640
gctcatgtcc aacattaccg ccatgttgac attgattatt gactagttat taatagtaat 17700
tccggatccg aaaacgtttt tcgaagctgc catagctaac cgagtacagg ttgtaatggc 17760
ggtacaactg taactaataa ctgatcaata attatcatta caattacggg gtcattagtt 17820
catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga 17880
ccgcccaacg acccccgccc gttaatgccc cagtaatcaa gtatcgggta tatacctcaa 17940
ggcgcaatgt attgaatgcc atttaccggg cggaccgact ggcgggttgc tgggggcggg 18000
attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg 18060
tcaatgggtg gagtatttac ggtaaactgc ccacttggca taactgcagt tattactgca 18120
tacaagggta tcattgcggt tatccctgaa aggtaactgc agttacccac ctcataaatg 18180
ccatttgacg ggtgaaccgt gtacatcaag tgtatcatat gccaagtacg ccccctattg 18240
acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 18300
catgtagttc acatagtata cggttcatgc gggggataac tgcagttact gccatttacc 18360
gggcggaccg taatacgggt catgtactgg aataccctga ttcctacttg gcagtacatc 18420
tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt 18480
ggatagcggt ttgactcacg aaggatgaac cgtcatgtag atgcataatc agtagcgata 18540
atggtaccac tacgccaaaa ccgtcatgta gttacccgca cctatcgcca aactgagtgc 18600
gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca 18660
acgggacttt ccaaaatgtc gtaacaactc cgccccattg ccctaaaggt tcagaggtgg 18720
ggtaactgca gttaccctca aacaaaaccg tggttttagt tgccctgaaa ggttttacag 18780
cattgttgag gcggggtaac acgcaaatgg gcggtaggcg tgtacggaat tcggagtggc 18840
gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctgtgcg 18900
tttacccgcc atccgcacat gccttaagcc tcaccgctcg ggagtctagg acgtatattc 18960
gtcgacgaaa aacggacatg acccagagag ac                               18992

<210> 58
<211> 679
<212> DNA
<213> Artificial Sequence

<220> 
<223> Long Spacer IgG4 hinge-CH2-CH3

<400> 58
tacggaccgt ccctgccccc cttgccctgc ccccgagttc ctgggcggac ccagcgtgtt 60
cctgttcccc cccaagccca aggacaccct gatgatcagc cggacccccg aggtgacctg 120
cgtggtggtg gacgtgagcc aggaagatcc cgaggtccag ttcaattggt acgtggacgg 180
cgtggaagtg cacaacgcca agaccaagcc cagagaggaa cagttcaaca gcacctaccg 240
ggtggtgtct gtgctgaccg tgctgcacca ggactggctg aacggcaaag aatacaagtg 300
caaggtgtcc aacaagggcc tgcccagcag catcgaaaag accatcagca aggccaaggg 360
ccagcctcgc gagccccagg tgtacaccct gcctccctcc caggaagaga tgaccaagaa 420
ccaggtgtcc ctgacctgcc tggtgaaggg cttctacccc agcgacatcg ccgtggagtg 480
ggagagcaac ggccagcctg agaacaacta caagaccacc cctcccgtgc tggacagcga 540
cggcagcttc ttcctgtaca gccggctgac cgtggacaag agccggtggc aggaaggcaa 600
cgtctttagc tgcagcgtga tgcacgaggc cctgcacaac cactacaccc agaagagcct 660
gagcctgtcc ctgggcaag                                              679

<210> 59
<211> 119
<212> PRT
<213> Artificial Sequence

<220> 
<223> Medium spacer

<400> 59
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Gly Gln Pro Arg
 1               5                  10                  15      
Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys
            20                  25                  30          
Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp
        35                  40                  45              
Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys
    50                  55                  60                  
Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser
65                  70                  75                  80  
Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser
                85                  90                  95      
Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser
            100                 105                 110         
Leu Ser Leu Ser Leu Gly Lys
        115                 


<210> 60
<211> 229
<212> PRT
<213> Artificial Sequence

<220> 
<223> Long spacer

<400> 60
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe
 1               5                  10                  15      
Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr
            20                  25                  30          
Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val
        35                  40                  45              
Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val
    50                  55                  60                  
Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser
65                  70                  75                  80  
Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
                85                  90                  95      
Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser
            100                 105                 110         
Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro
        115                 120                 125             
Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln
    130                 135                 140                 
Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala
145                 150                 155                 160 
Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr
                165                 170                 175     
Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu
            180                 185                 190         
Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser
        195                 200                 205             
Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser
    210                 215                 220                 
Leu Ser Leu Gly Lys
225                 

