                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  Detergent composition

<130>  12896-WO-PCT

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  868
<212>  DNA
<213>  Trichoderma harzianum


<220>
<221>  exon
<222>  (1)..(75)

<220>
<221>  Intron
<222>  (76)..(154)

<220>
<221>  exon
<222>  (155)..(288)

<220>
<221>  Intron
<222>  (289)..(362)

<220>
<221>  exon
<222>  (363)..(519)

<220>
<221>  Intron
<222>  (520)..(615)

<220>
<221>  exon
<222>  (616)..(867)

<400>  1
atg aag ctg tcc atc tct gtc gct ctt act tcg gcc atc gcg gtt ctc         48
Met Lys Leu Ser Ile Ser Val Ala Leu Thr Ser Ala Ile Ala Val Leu           
1               5                   10                  15                

gcc gcc ccg gct cct atg cct aca ccg gtatgtagca tcaatgcaac               95
Ala Ala Pro Ala Pro Met Pro Thr Pro                                       
            20                  25                                        

atgacataac ttgtatctcg actatatatc agactggcta atgcttcaac tcattacag       154

ccc ggt att ccc acg gaa agc agc gcc aga acc caa ctt gcc ggc ctg        202
Pro Gly Ile Pro Thr Glu Ser Ser Ala Arg Thr Gln Leu Ala Gly Leu           
                30                  35                  40                

act gtt gcc gtt gct ggc tct gga act ggt tac tcc cgc gac ctg ttt        250
Thr Val Ala Val Ala Gly Ser Gly Thr Gly Tyr Ser Arg Asp Leu Phe           
            45                  50                  55                    

ccc act tgg gat gcc atc tct ggt aac tgc aac gct cg  gtatgataac         298
Pro Thr Trp Asp Ala Ile Ser Gly Asn Cys Asn Ala Arg                       
        60                  65                  70                        

atcctaggac ctttcaagct tcggaaatac aacacaaagg ctaacaaagt ggatgtgcaa      358

atag c gaa tat gtg ttg aag cga gat ggt gaa ggt gtc caa gtc aac         405
       Glu Tyr Val Leu Lys Arg Asp Gly Glu Gly Val Gln Val Asn            
                       75                  80                             

aat gct tgt gaa tct cag tcc ggc acc tgg atc aga tcc tta tga caa        453
Asn Ala Cys Glu Ser Gln Ser Gly Thr Trp Ile Arg Ser Leu     Gln           
85                  90                  95                                

cgc cag ttt cac aaa tgc atc cag ctt gga tat tga cca cat ggt gcc        501
Arg Gln Phe His Lys Cys Ile Gln Leu Gly Tyr     Pro His Gly Ala           
100                 105                 110                               

tct aaa gaa tgc ctg gat cgtgagtttt ctcctttttc actgcgtatc               549
Ser Lys Glu Cys Leu Asp                                                   
115                 120                                                   

tccgttccct acctttttgc gatactatat catgccacat cactaatatg gacaaatttc      609

tcgcca gtc cgg tgc ctc aag ctg gac cac agc cca acg tga agc cct         657
       Val Arg Cys Leu Lys Leu Asp His Ser Pro Thr     Ser Pro            
                       125                 130                            

cgc caa cga cgt ctc ccg tcc cca act ctg ggc cgt ctc cgc aag cgc        705
Arg Gln Arg Arg Leu Pro Ser Pro Thr Leu Gly Arg Leu Arg Lys Arg           
    135                 140                 145                           

aaa ccg ctc caa ggg cga ccg cag ccc aga cca gtg gaa gcc tcc tct        753
Lys Pro Leu Gln Gly Arg Pro Gln Pro Arg Pro Val Glu Ala Ser Ser           
150                 155                 160                 165           

gac cag ctt cta ctg cac cta cgc caa gtc gtg gat cga tgt caa gag        801
Asp Gln Leu Leu Leu His Leu Arg Gln Val Val Asp Arg Cys Gln Glu           
                170                 175                 180               

ctt cta taa gct gac aat cac cag tgc cga gaa gac agc tct gag cag        849
Leu Leu     Ala Asp Asn His Gln Cys Arg Glu Asp Ser Ser Glu Gln           
                185                 190                 195               

cat gtt aga tac ttg cta g                                              868
His Val Arg Tyr Leu Leu                                                   
            200                                                           


<210>  2
<211>  205
<212>  PRT
<213>  Trichoderma harzianum


<220>
<221>  SIGNAL
<222>  (1)..(17)

<220>
<221>  mat_peptide
<222>  (18)..(205)

<400>  2

Met Lys Leu Ser Ile Ser Val Ala Leu Thr Ser Ala Ile Ala Val Leu 
        -15                 -10                 -5              


Ala Ala Pro Ala Pro Met Pro Thr Pro Pro Gly Ile Pro Thr Glu Ser 
-1  1               5                   10                  15  


Ser Ala Arg Thr Gln Leu Ala Gly Leu Thr Val Ala Val Ala Gly Ser 
                20                  25                  30      


Gly Thr Gly Tyr Ser Arg Asp Leu Phe Pro Thr Trp Asp Ala Ile Ser 
            35                  40                  45          


Gly Asn Cys Asn Ala Arg Glu Tyr Val Leu Lys Arg Asp Gly Glu Gly 
        50                  55                  60              


Val Gln Val Asn Asn Ala Cys Glu Ser Gln Ser Gly Thr Trp Ile Ser 
    65                  70                  75                  


Pro Tyr Asp Asn Ala Ser Phe Thr Asn Ala Ser Ser Leu Asp Ile Asp 
80                  85                  90                  95  


His Met Val Pro Leu Lys Asn Ala Trp Ile Ser Gly Ala Ser Ser Trp 
                100                 105                 110     


Thr Thr Ala Gln Arg Glu Ala Leu Ala Asn Asp Val Ser Arg Pro Gln 
            115                 120                 125         


Leu Trp Ala Val Ser Ala Ser Ala Asn Arg Ser Lys Gly Asp Arg Ser 
        130                 135                 140             


Pro Asp Gln Trp Lys Pro Pro Leu Thr Ser Phe Tyr Cys Thr Tyr Ala 
    145                 150                 155                 


Lys Ser Trp Ile Asp Val Lys Ser Phe Tyr Lys Leu Thr Ile Thr Ser 
160                 165                 170                 175 


Ala Glu Lys Thr Ala Leu Ser Ser Met Leu Asp Thr Cys 
                180                 185             


<210>  3
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  3
acacaactgg ggatccacca tgaagctgtc catctctgtc g                           41


<210>  4
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  4
agatctcgag aagcttacta gcaagtatct aacatgctgc t                           41


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  5
gcaagggatg ccatgcttgg                                                   20


<210>  6
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  6
catataacca attgccctc                                                    19


<210>  7
<211>  269
<212>  PRT
<213>  Substilisin 309

<400>  7

Ala Gln Ser Val Pro Trp Gly Ile Ser Arg Val Gln Ala Pro Ala Ala 
1               5                   10                  15      


His Asn Arg Gly Leu Thr Gly Ser Gly Val Lys Val Ala Val Leu Asp 
            20                  25                  30          


Thr Gly Ile Ser Thr His Pro Asp Leu Asn Ile Arg Gly Gly Ala Ser 
        35                  40                  45              


Phe Val Pro Gly Glu Pro Ser Thr Gln Asp Gly Asn Gly His Gly Thr 
    50                  55                  60                  


His Val Ala Gly Thr Ile Ala Ala Leu Asn Asn Ser Ile Gly Val Leu 
65                  70                  75                  80  


Gly Val Ala Pro Ser Ala Glu Leu Tyr Ala Val Lys Val Leu Gly Ala 
                85                  90                  95      


Ser Gly Ser Gly Ser Val Ser Ser Ile Ala Gln Gly Leu Glu Trp Ala 
            100                 105                 110         


Gly Asn Asn Gly Met His Val Ala Asn Leu Ser Leu Gly Ser Pro Ser 
        115                 120                 125             


Pro Ser Ala Thr Leu Glu Gln Ala Val Asn Ser Ala Thr Ser Arg Gly 
    130                 135                 140                 


Val Leu Val Val Ala Ala Ser Gly Asn Ser Gly Ala Gly Ser Ile Ser 
145                 150                 155                 160 


Tyr Pro Ala Arg Tyr Ala Asn Ala Met Ala Val Gly Ala Thr Asp Gln 
                165                 170                 175     


Asn Asn Asn Arg Ala Ser Phe Ser Gln Tyr Gly Ala Gly Leu Asp Ile 
            180                 185                 190         


Val Ala Pro Gly Val Asn Val Gln Ser Thr Tyr Pro Gly Ser Thr Tyr 
        195                 200                 205             


Ala Ser Leu Asn Gly Thr Ser Met Ala Thr Pro His Val Ala Gly Ala 
    210                 215                 220                 


Ala Ala Leu Val Lys Gln Lys Asn Pro Ser Trp Ser Asn Val Gln Ile 
225                 230                 235                 240 


Arg Asn His Leu Lys Asn Thr Ala Thr Ser Leu Gly Ser Thr Asn Leu 
                245                 250                 255     


Tyr Gly Ser Gly Leu Val Asn Ala Glu Ala Ala Thr Arg 
            260                 265                 


<210>  8
<211>  618
<212>  DNA
<213>  Trichoderma harzianum

<400>  8
atgaagctgt ccatctctgt cgctcttact tcggccatcg cggttctcgc cgccccggct       60

cctatgccta caccgcccgg tattcccacg gaaagcagcg ccagaaccca acttgccggc      120

ctgactgttg ccgttgctgg ctctggaact ggttactccc gcgacctgtt tcccacttgg      180

gatgccatct ctggtaactg caacgctcgc gaatatgtgt tgaagcgaga tggtgaaggt      240

gtccaagtca acaatgcttg tgaatctcag tccggcacct ggatcagtcc ttatgacaac      300

gccagtttca caaatgcatc cagcttggat attgaccaca tggtgcctct aaagaatgcc      360

tggatctccg gtgcctcaag ctggaccaca gcccaacgtg aagccctcgc caacgacgtc      420

tcccgtcccc aactctgggc cgtctccgca agcgcaaacc gctccaaggg cgaccgcagc      480

ccagaccagt ggaagcctcc tctgaccagc ttctactgca cctacgccaa gtcgtggatc      540

gatgtcaaga gcttctataa gctgacaatc accagtgccg agaagacagc tctgagcagc      600

atgttagata cttgctag                                                    618


