                         SEQUENCE LISTING

<110>  CELLECTIS
 
<120>  METHOD FOR GENERATING IMMUNE CELLS RESISTANT TO ARGININE AND/OR 
       TRYPTOPHAN DEPLETED MICROENVIRONMENT

<130>  P81401593PCT00

<150>  PA201470209
<151>  2014-04-11

<160>  53    

<170>  PatentIn version 3.5

<210>  1
<211>  1649
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Ala Gly Gly Arg Gly Ala Pro Gly Arg Gly Arg Asp Glu Pro Pro 
1               5                   10                  15      


Glu Ser Tyr Pro Gln Arg Gln Asp His Glu Leu Gln Ala Leu Glu Ala 
            20                  25                  30          


Ile Tyr Gly Ala Asp Phe Gln Asp Leu Arg Pro Asp Ala Cys Gly Pro 
        35                  40                  45              


Val Lys Glu Pro Pro Glu Ile Asn Leu Val Leu Tyr Pro Gln Gly Leu 
    50                  55                  60                  


Thr Gly Glu Glu Val Tyr Val Lys Val Asp Leu Arg Val Lys Cys Pro 
65                  70                  75                  80  


Pro Thr Tyr Pro Asp Val Val Pro Glu Ile Glu Leu Lys Asn Ala Lys 
                85                  90                  95      


Gly Leu Ser Asn Glu Ser Val Asn Leu Leu Lys Ser Arg Leu Glu Glu 
            100                 105                 110         


Leu Ala Lys Lys His Cys Gly Glu Val Met Ile Phe Glu Leu Ala Tyr 
        115                 120                 125             


His Val Gln Ser Phe Leu Ser Glu His Asn Lys Pro Pro Pro Lys Ser 
    130                 135                 140                 


Phe His Glu Glu Met Leu Glu Arg Arg Ala Gln Glu Glu Gln Gln Arg 
145                 150                 155                 160 


Leu Leu Glu Ala Lys Arg Lys Glu Glu Gln Glu Gln Arg Glu Ile Leu 
                165                 170                 175     


His Glu Ile Gln Arg Arg Lys Glu Glu Ile Lys Glu Glu Lys Lys Arg 
            180                 185                 190         


Lys Glu Met Ala Lys Gln Glu Arg Leu Glu Ile Ala Ser Leu Ser Asn 
        195                 200                 205             


Gln Asp His Thr Ser Lys Lys Asp Pro Gly Gly His Arg Thr Ala Ala 
    210                 215                 220                 


Ile Leu His Gly Gly Ser Pro Asp Phe Val Gly Asn Gly Lys His Arg 
225                 230                 235                 240 


Ala Asn Ser Ser Gly Arg Ser Arg Arg Glu Arg Gln Tyr Ser Val Cys 
                245                 250                 255     


Asn Ser Glu Asp Ser Pro Gly Ser Cys Glu Ile Leu Tyr Phe Asn Met 
            260                 265                 270         


Gly Ser Pro Asp Gln Leu Met Val His Lys Gly Lys Cys Ile Gly Ser 
        275                 280                 285             


Asp Glu Gln Leu Gly Lys Leu Val Tyr Asn Ala Leu Glu Thr Ala Thr 
    290                 295                 300                 


Gly Gly Phe Val Leu Leu Tyr Glu Trp Val Leu Gln Trp Gln Lys Lys 
305                 310                 315                 320 


Met Gly Pro Phe Leu Thr Ser Gln Glu Lys Glu Lys Ile Asp Lys Cys 
                325                 330                 335     


Lys Lys Gln Ile Gln Gly Thr Glu Thr Glu Phe Asn Ser Leu Val Lys 
            340                 345                 350         


Leu Ser His Pro Asn Val Val Arg Tyr Leu Ala Met Asn Leu Lys Glu 
        355                 360                 365             


Gln Asp Asp Ser Ile Val Val Asp Ile Leu Val Glu His Ile Ser Gly 
    370                 375                 380                 


Val Ser Leu Ala Ala His Leu Ser His Ser Gly Pro Ile Pro Val His 
385                 390                 395                 400 


Gln Leu Arg Arg Tyr Thr Ala Gln Leu Leu Ser Gly Leu Asp Tyr Leu 
                405                 410                 415     


His Ser Asn Ser Val Val His Lys Val Leu Ser Ala Ser Asn Val Leu 
            420                 425                 430         


Val Asp Ala Glu Gly Thr Val Lys Ile Thr Asp Tyr Ser Ile Ser Lys 
        435                 440                 445             


Arg Leu Ala Asp Ile Cys Lys Glu Asp Val Phe Glu Gln Thr Arg Val 
    450                 455                 460                 


Arg Phe Ser Asp Asn Ala Leu Pro Tyr Lys Thr Gly Lys Lys Gly Asp 
465                 470                 475                 480 


Val Trp Arg Leu Gly Leu Leu Leu Leu Ser Leu Ser Gln Gly Gln Glu 
                485                 490                 495     


Cys Gly Glu Tyr Pro Val Thr Ile Pro Ser Asp Leu Pro Ala Asp Phe 
            500                 505                 510         


Gln Asp Phe Leu Lys Lys Cys Val Cys Leu Asp Asp Lys Glu Arg Trp 
        515                 520                 525             


Ser Pro Gln Gln Leu Leu Lys His Ser Phe Ile Asn Pro Gln Pro Lys 
    530                 535                 540                 


Met Pro Leu Val Glu Gln Ser Pro Glu Asp Ser Glu Gly Gln Asp Tyr 
545                 550                 555                 560 


Val Glu Thr Val Ile Pro Ser Asn Arg Leu Pro Ser Ala Ala Phe Phe 
                565                 570                 575     


Ser Glu Thr Gln Arg Gln Phe Ser Arg Tyr Phe Ile Glu Phe Glu Glu 
            580                 585                 590         


Leu Gln Leu Leu Gly Lys Gly Ala Phe Gly Ala Val Ile Lys Val Gln 
        595                 600                 605             


Asn Lys Leu Asp Gly Cys Cys Tyr Ala Val Lys Arg Ile Pro Ile Asn 
    610                 615                 620                 


Pro Ala Ser Arg Gln Phe Arg Arg Ile Lys Gly Glu Val Thr Leu Leu 
625                 630                 635                 640 


Ser Arg Leu His His Glu Asn Ile Val Arg Tyr Tyr Asn Ala Trp Ile 
                645                 650                 655     


Glu Arg His Glu Arg Pro Ala Gly Pro Gly Thr Pro Pro Pro Asp Ser 
            660                 665                 670         


Gly Pro Leu Ala Lys Asp Asp Arg Ala Ala Arg Gly Gln Pro Ala Ser 
        675                 680                 685             


Asp Thr Asp Gly Leu Asp Ser Val Glu Ala Ala Ala Pro Pro Pro Ile 
    690                 695                 700                 


Leu Ser Ser Ser Val Glu Trp Ser Thr Ser Gly Glu Arg Ser Ala Ser 
705                 710                 715                 720 


Ala Arg Phe Pro Ala Thr Gly Pro Gly Ser Ser Asp Asp Glu Asp Asp 
                725                 730                 735     


Asp Glu Asp Glu His Gly Gly Val Phe Ser Gln Ser Phe Leu Pro Ala 
            740                 745                 750         


Ser Asp Ser Glu Ser Asp Ile Ile Phe Asp Asn Glu Asp Glu Asn Ser 
        755                 760                 765             


Lys Ser Gln Asn Gln Asp Glu Asp Cys Asn Glu Lys Asn Gly Cys His 
    770                 775                 780                 


Glu Ser Glu Pro Ser Val Thr Thr Glu Ala Val His Tyr Leu Tyr Ile 
785                 790                 795                 800 


Gln Met Glu Tyr Cys Glu Lys Ser Thr Leu Arg Asp Thr Ile Asp Gln 
                805                 810                 815     


Gly Leu Tyr Arg Asp Thr Val Arg Leu Trp Arg Leu Phe Arg Glu Ile 
            820                 825                 830         


Leu Asp Gly Leu Ala Tyr Ile His Glu Lys Gly Met Ile His Arg Asp 
        835                 840                 845             


Leu Lys Pro Val Asn Ile Phe Leu Asp Ser Asp Asp His Val Lys Ile 
    850                 855                 860                 


Gly Asp Phe Gly Leu Ala Thr Asp His Leu Ala Phe Ser Ala Asp Ser 
865                 870                 875                 880 


Lys Gln Asp Asp Gln Thr Gly Asp Leu Ile Lys Ser Asp Pro Ser Gly 
                885                 890                 895     


His Leu Thr Gly Met Val Gly Thr Ala Leu Tyr Val Ser Pro Glu Val 
            900                 905                 910         


Gln Gly Ser Thr Lys Ser Ala Tyr Asn Gln Lys Val Asp Leu Phe Ser 
        915                 920                 925             


Leu Gly Ile Ile Phe Phe Glu Met Ser Tyr His Pro Met Val Thr Ala 
    930                 935                 940                 


Ser Glu Arg Ile Phe Val Leu Asn Gln Leu Arg Asp Pro Thr Ser Pro 
945                 950                 955                 960 


Lys Phe Pro Glu Asp Phe Asp Asp Gly Glu His Ala Lys Gln Lys Ser 
                965                 970                 975     


Val Ile Ser Trp Leu Leu Asn His Asp Pro Ala Lys Arg Pro Thr Ala 
            980                 985                 990         


Thr Glu Leu Leu Lys Ser Glu Leu  Leu Pro Pro Pro Gln  Met Glu Glu 
        995                 1000                 1005             


Ser Glu  Leu His Glu Val Leu  His His Thr Leu Thr  Asn Val Asp 
    1010                 1015                 1020             


Gly Lys  Ala Tyr Arg Thr Met  Met Ala Gln Ile Phe  Ser Gln Arg 
    1025                 1030                 1035             


Ile Ser  Pro Ala Ile Asp Tyr  Thr Tyr Asp Ser Asp  Ile Leu Lys 
    1040                 1045                 1050             


Gly Asn  Phe Ser Ile Arg Thr  Ala Lys Met Gln Gln  His Val Cys 
    1055                 1060                 1065             


Glu Thr  Ile Ile Arg Ile Phe  Lys Arg His Gly Ala  Val Gln Leu 
    1070                 1075                 1080             


Cys Thr  Pro Leu Leu Leu Pro  Arg Asn Arg Gln Ile  Tyr Glu His 
    1085                 1090                 1095             


Asn Glu  Ala Ala Leu Phe Met  Asp His Ser Gly Met  Leu Val Met 
    1100                 1105                 1110             


Leu Pro  Phe Asp Leu Arg Ile  Pro Phe Ala Arg Tyr  Val Ala Arg 
    1115                 1120                 1125             


Asn Asn  Ile Leu Asn Leu Lys  Arg Tyr Cys Ile Glu  Arg Val Phe 
    1130                 1135                 1140             


Arg Pro  Arg Lys Leu Asp Arg  Phe His Pro Lys Glu  Leu Leu Glu 
    1145                 1150                 1155             


Cys Ala  Phe Asp Ile Val Thr  Ser Thr Thr Asn Ser  Phe Leu Pro 
    1160                 1165                 1170             


Thr Ala  Glu Ile Ile Tyr Thr  Ile Tyr Glu Ile Ile  Gln Glu Phe 
    1175                 1180                 1185             


Pro Ala  Leu Gln Glu Arg Asn  Tyr Ser Ile Tyr Leu  Asn His Thr 
    1190                 1195                 1200             


Met Leu  Leu Lys Ala Ile Leu  Leu His Cys Gly Ile  Pro Glu Asp 
    1205                 1210                 1215             


Lys Leu  Ser Gln Val Tyr Ile  Ile Leu Tyr Asp Ala  Val Thr Glu 
    1220                 1225                 1230             


Lys Leu  Thr Arg Arg Glu Val  Glu Ala Lys Phe Cys  Asn Leu Ser 
    1235                 1240                 1245             


Leu Ser  Ser Asn Ser Leu Cys  Arg Leu Tyr Lys Phe  Ile Glu Gln 
    1250                 1255                 1260             


Lys Gly  Asp Leu Gln Asp Leu  Met Pro Thr Ile Asn  Ser Leu Ile 
    1265                 1270                 1275             


Lys Gln  Lys Thr Gly Ile Ala  Gln Leu Val Lys Tyr  Gly Leu Lys 
    1280                 1285                 1290             


Asp Leu  Glu Glu Val Val Gly  Leu Leu Lys Lys Leu  Gly Ile Lys 
    1295                 1300                 1305             


Leu Gln  Val Leu Ile Asn Leu  Gly Leu Val Tyr Lys  Val Gln Gln 
    1310                 1315                 1320             


His Asn  Gly Ile Ile Phe Gln  Phe Val Ala Phe Ile  Lys Arg Arg 
    1325                 1330                 1335             


Gln Arg  Ala Val Pro Glu Ile  Leu Ala Ala Gly Gly  Arg Tyr Asp 
    1340                 1345                 1350             


Leu Leu  Ile Pro Gln Phe Arg  Gly Pro Gln Ala Leu  Gly Pro Val 
    1355                 1360                 1365             


Pro Thr  Ala Ile Gly Val Ser  Ile Ala Ile Asp Lys  Ile Ser Ala 
    1370                 1375                 1380             


Ala Val  Leu Asn Met Glu Glu  Ser Val Thr Ile Ser  Ser Cys Asp 
    1385                 1390                 1395             


Leu Leu  Val Val Ser Val Gly  Gln Met Ser Met Ser  Arg Ala Ile 
    1400                 1405                 1410             


Asn Leu  Thr Gln Lys Leu Trp  Thr Ala Gly Ile Thr  Ala Glu Ile 
    1415                 1420                 1425             


Met Tyr  Asp Trp Ser Gln Ser  Gln Glu Glu Leu Gln  Glu Tyr Cys 
    1430                 1435                 1440             


Arg His  His Glu Ile Thr Tyr  Val Ala Leu Val Ser  Asp Lys Glu 
    1445                 1450                 1455             


Gly Ser  His Val Lys Val Lys  Ser Phe Glu Lys Glu  Arg Gln Thr 
    1460                 1465                 1470             


Glu Lys  Arg Val Leu Glu Thr  Glu Leu Val Asp His  Val Leu Gln 
    1475                 1480                 1485             


Lys Leu  Arg Thr Lys Val Thr  Asp Glu Arg Asn Gly  Arg Glu Ala 
    1490                 1495                 1500             


Ser Asp  Asn Leu Ala Val Gln  Asn Leu Lys Gly Ser  Phe Ser Asn 
    1505                 1510                 1515             


Ala Ser  Gly Leu Phe Glu Ile  His Gly Ala Thr Val  Val Pro Ile 
    1520                 1525                 1530             


Val Ser  Val Leu Ala Pro Glu  Lys Leu Ser Ala Ser  Thr Arg Arg 
    1535                 1540                 1545             


Arg Tyr  Glu Thr Gln Val Gln  Thr Arg Leu Gln Thr  Ser Leu Ala 
    1550                 1555                 1560             


Asn Leu  His Gln Lys Ser Ser  Glu Ile Glu Ile Leu  Ala Val Asp 
    1565                 1570                 1575             


Leu Pro  Lys Glu Thr Ile Leu  Gln Phe Leu Ser Leu  Glu Trp Asp 
    1580                 1585                 1590             


Ala Asp  Glu Gln Ala Phe Asn  Thr Thr Val Lys Gln  Leu Leu Ser 
    1595                 1600                 1605             


Arg Leu  Pro Lys Gln Arg Tyr  Leu Lys Leu Val Cys  Asp Glu Ile 
    1610                 1615                 1620             


Tyr Asn  Ile Lys Val Glu Lys  Lys Val Ser Val Leu  Phe Leu Tyr 
    1625                 1630                 1635             


Ser Tyr  Arg Asp Asp Tyr Tyr  Arg Ile Leu Phe 
    1640                 1645                 


<210>  2
<211>  825
<212>  PRT
<213>  Homo sapiens

<400>  2

Met Leu Asp Ile Cys Leu Glu Lys Arg Val Gly Thr Thr Leu Ala Ala 
1               5                   10                  15      


Pro Lys Cys Asn Ser Ser Thr Val Arg Phe Gln Gly Leu Ala Glu Gly 
            20                  25                  30          


Thr Lys Gly Thr Met Lys Met Asp Met Glu Asp Ala Asp Met Thr Leu 
        35                  40                  45              


Trp Thr Glu Ala Glu Phe Glu Glu Lys Cys Thr Tyr Ile Val Asn Asp 
    50                  55                  60                  


His Pro Trp Asp Ser Gly Ala Asp Gly Gly Thr Ser Val Gln Ala Glu 
65                  70                  75                  80  


Ala Ser Leu Pro Arg Asn Leu Leu Phe Lys Tyr Ala Thr Asn Ser Glu 
                85                  90                  95      


Glu Val Ile Gly Val Met Ser Lys Glu Tyr Ile Pro Lys Gly Thr Arg 
            100                 105                 110         


Phe Gly Pro Leu Ile Gly Glu Ile Tyr Thr Asn Asp Thr Val Pro Lys 
        115                 120                 125             


Asn Ala Asn Arg Lys Tyr Phe Trp Arg Ile Tyr Ser Arg Gly Glu Leu 
    130                 135                 140                 


His His Phe Ile Asp Gly Phe Asn Glu Glu Lys Ser Asn Trp Met Arg 
145                 150                 155                 160 


Tyr Val Asn Pro Ala His Ser Pro Arg Glu Gln Asn Leu Ala Ala Cys 
                165                 170                 175     


Gln Asn Gly Met Asn Ile Tyr Phe Tyr Thr Ile Lys Pro Ile Pro Ala 
            180                 185                 190         


Asn Gln Glu Leu Leu Val Trp Tyr Cys Arg Asp Phe Ala Glu Arg Leu 
        195                 200                 205             


His Tyr Pro Tyr Pro Gly Glu Leu Thr Met Met Asn Leu Thr Gln Thr 
    210                 215                 220                 


Gln Ser Ser Leu Lys Gln Pro Ser Thr Glu Lys Asn Glu Leu Cys Pro 
225                 230                 235                 240 


Lys Asn Val Pro Lys Arg Glu Tyr Ser Val Lys Glu Ile Leu Lys Leu 
                245                 250                 255     


Asp Ser Asn Pro Ser Lys Gly Lys Asp Leu Tyr Arg Ser Asn Ile Ser 
            260                 265                 270         


Pro Leu Thr Ser Glu Lys Asp Leu Asp Asp Phe Arg Arg Arg Gly Ser 
        275                 280                 285             


Pro Glu Met Pro Phe Tyr Pro Arg Val Val Tyr Pro Ile Arg Ala Pro 
    290                 295                 300                 


Leu Pro Glu Asp Phe Leu Lys Ala Ser Leu Ala Tyr Gly Ile Glu Arg 
305                 310                 315                 320 


Pro Thr Tyr Ile Thr Arg Ser Pro Ile Pro Ser Ser Thr Thr Pro Ser 
                325                 330                 335     


Pro Ser Ala Arg Ser Ser Pro Asp Gln Ser Leu Lys Ser Ser Ser Pro 
            340                 345                 350         


His Ser Ser Pro Gly Asn Thr Val Ser Pro Val Gly Pro Gly Ser Gln 
        355                 360                 365             


Glu His Arg Asp Ser Tyr Ala Tyr Leu Asn Ala Ser Tyr Gly Thr Glu 
    370                 375                 380                 


Gly Leu Gly Ser Tyr Pro Gly Tyr Ala Pro Leu Pro His Leu Pro Pro 
385                 390                 395                 400 


Ala Phe Ile Pro Ser Tyr Asn Ala His Tyr Pro Lys Phe Leu Leu Pro 
                405                 410                 415     


Pro Tyr Gly Met Asn Cys Asn Gly Leu Ser Ala Val Ser Ser Met Asn 
            420                 425                 430         


Gly Ile Asn Asn Phe Gly Leu Phe Pro Arg Leu Cys Pro Val Tyr Ser 
        435                 440                 445             


Asn Leu Leu Gly Gly Gly Ser Leu Pro His Pro Met Leu Asn Pro Thr 
    450                 455                 460                 


Ser Leu Pro Ser Ser Leu Pro Ser Asp Gly Ala Arg Arg Leu Leu Gln 
465                 470                 475                 480 


Pro Glu His Pro Arg Glu Val Leu Val Pro Ala Pro His Ser Ala Phe 
                485                 490                 495     


Ser Phe Thr Gly Ala Ala Ala Ser Met Lys Asp Lys Ala Cys Ser Pro 
            500                 505                 510         


Thr Ser Gly Ser Pro Thr Ala Gly Thr Ala Ala Thr Ala Glu His Val 
        515                 520                 525             


Val Gln Pro Lys Ala Thr Ser Ala Ala Met Ala Ala Pro Ser Ser Asp 
    530                 535                 540                 


Glu Ala Met Asn Leu Ile Lys Asn Lys Arg Asn Met Thr Gly Tyr Lys 
545                 550                 555                 560 


Thr Leu Pro Tyr Pro Leu Lys Lys Gln Asn Gly Lys Ile Lys Tyr Glu 
                565                 570                 575     


Cys Asn Val Cys Ala Lys Thr Phe Gly Gln Leu Ser Asn Leu Lys Val 
            580                 585                 590         


His Leu Arg Val His Ser Gly Glu Arg Pro Phe Lys Cys Gln Thr Cys 
        595                 600                 605             


Asn Lys Gly Phe Thr Gln Leu Ala His Leu Gln Lys His Tyr Leu Val 
    610                 615                 620                 


His Thr Gly Glu Lys Pro His Glu Cys Gln Val Cys His Lys Arg Phe 
625                 630                 635                 640 


Ser Ser Thr Ser Asn Leu Lys Thr His Leu Arg Leu His Ser Gly Glu 
                645                 650                 655     


Lys Pro Tyr Gln Cys Lys Val Cys Pro Ala Lys Phe Thr Gln Phe Val 
            660                 665                 670         


His Leu Lys Leu His Lys Arg Leu His Thr Arg Glu Arg Pro His Lys 
        675                 680                 685             


Cys Ser Gln Cys His Lys Asn Tyr Ile His Leu Cys Ser Leu Lys Val 
    690                 695                 700                 


His Leu Lys Gly Asn Cys Ala Ala Ala Pro Ala Pro Gly Leu Pro Leu 
705                 710                 715                 720 


Glu Asp Leu Thr Arg Ile Asn Glu Glu Ile Glu Lys Phe Asp Ile Ser 
                725                 730                 735     


Asp Asn Ala Asp Arg Leu Glu Asp Val Glu Asp Asp Ile Ser Val Ile 
            740                 745                 750         


Ser Val Val Glu Lys Glu Ile Leu Ala Val Val Arg Lys Glu Lys Glu 
        755                 760                 765             


Glu Thr Gly Leu Lys Val Ser Leu Gln Arg Asn Met Gly Asn Gly Leu 
    770                 775                 780                 


Leu Ser Ser Gly Cys Ser Leu Tyr Glu Ser Ser Asp Leu Pro Leu Met 
785                 790                 795                 800 


Lys Leu Pro Pro Ser Asn Pro Leu Pro Leu Val Pro Val Lys Val Lys 
                805                 810                 815     


Gln Glu Thr Val Glu Pro Met Asp Pro 
            820                 825 


<210>  3
<211>  49
<212>  DNA
<213>  Homo sapiens

<400>  3
tggatttgag ggttaaatgc ccacctacct atccagatgt gtgagtaca                   49


<210>  4
<211>  49
<212>  DNA
<213>  Homo sapiens

<400>  4
ttgtaggaaa tggtaaacat cgggcaaact cctcaggaag gtctaggta                   49


<210>  5
<211>  495
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-CD19 Cimeric Antigen Receptor

<400>  5

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Ile 
            20                  25                  30          


Lys Pro Gly Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr 
        35                  40                  45              


Phe Thr Ser Tyr Val Met His Trp Val Lys Gln Lys Pro Gly Gln Gly 
    50                  55                  60                  


Leu Glu Trp Ile Gly Tyr Ile Asn Pro Tyr Asn Asp Gly Thr Lys Tyr 
65                  70                  75                  80  


Asn Glu Lys Phe Lys Gly Lys Ala Thr Leu Thr Ser Asp Lys Ser Ser 
                85                  90                  95      


Ser Thr Ala Tyr Met Glu Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala 
            100                 105                 110         


Val Tyr Tyr Cys Ala Arg Gly Thr Tyr Tyr Tyr Gly Ser Arg Val Phe 
        115                 120                 125             


Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Gly Gly Gly 
    130                 135                 140                 


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Met 
145                 150                 155                 160 


Thr Gln Ala Ala Pro Ser Ile Pro Val Thr Pro Gly Glu Ser Val Ser 
                165                 170                 175     


Ile Ser Cys Arg Ser Ser Lys Ser Leu Leu Asn Ser Asn Gly Asn Thr 
            180                 185                 190         


Tyr Leu Tyr Trp Phe Leu Gln Arg Pro Gly Gln Ser Pro Gln Leu Leu 
        195                 200                 205             


Ile Tyr Arg Met Ser Asn Leu Ala Ser Gly Val Pro Asp Arg Phe Ser 
    210                 215                 220                 


Gly Ser Gly Ser Gly Thr Ala Phe Thr Leu Arg Ile Ser Arg Val Glu 
225                 230                 235                 240 


Ala Glu Asp Val Gly Val Tyr Tyr Cys Met Gln His Leu Glu Tyr Pro 
                245                 250                 255     


Phe Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys Arg Ser Asp Pro 
            260                 265                 270         


Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
        275                 280                 285             


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
    290                 295                 300                 


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile 
305                 310                 315                 320 


Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val 
                325                 330                 335     


Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
            340                 345                 350         


Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
        355                 360                 365             


Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg 
    370                 375                 380                 


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln 
385                 390                 395                 400 


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
                405                 410                 415     


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
            420                 425                 430         


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
        435                 440                 445             


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
    450                 455                 460                 


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
465                 470                 475                 480 


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490                 495 

<210>  6
<211>  251
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-CD19 CAR

<400>  6

Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Ile Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr 
            20                  25                  30          


Val Met His Trp Val Lys Gln Lys Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Tyr Asn Asp Gly Thr Lys Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ser Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Thr Tyr Tyr Tyr Gly Ser Arg Val Phe Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Thr Leu Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Gly Gly Gly Gly Ser Asp Ile Val Met Thr Gln Ala Ala 
    130                 135                 140                 


Pro Ser Ile Pro Val Thr Pro Gly Glu Ser Val Ser Ile Ser Cys Arg 
145                 150                 155                 160 


Ser Ser Lys Ser Leu Leu Asn Ser Asn Gly Asn Thr Tyr Leu Tyr Trp 
                165                 170                 175     


Phe Leu Gln Arg Pro Gly Gln Ser Pro Gln Leu Leu Ile Tyr Arg Met 
            180                 185                 190         


Ser Asn Leu Ala Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser 
        195                 200                 205             


Gly Thr Ala Phe Thr Leu Arg Ile Ser Arg Val Glu Ala Glu Asp Val 
    210                 215                 220                 


Gly Val Tyr Tyr Cys Met Gln His Leu Glu Tyr Pro Phe Thr Phe Gly 
225                 230                 235                 240 


Ala Gly Thr Lys Leu Glu Leu Lys Arg Ala Asp 
                245                 250     


<210>  7
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  7
gagaatcaaa atcggtgaat agg                                               23


<210>  8
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  8
ttcaaaacct gtcagtgatt ggg                                               23


<210>  9
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  9
tgtgctagac atgaggtcta tgg                                               23


<210>  10
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  10
cgtcatgagc agattaaacc cgg                                               23


<210>  11
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  11
tcagggttct ggatatctgt ggg                                               23


<210>  12
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  12
gtcagggttc tggatatctg tgg                                               23


<210>  13
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  13
ttcggaaccc aatcactgac agg                                               23


<210>  14
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  14
taaacccggc cactttcagg agg                                               23


<210>  15
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  15
aaagtcagat ttgttgctcc agg                                               23


<210>  16
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  16
aacaaatgtg tcacaaagta agg                                               23


<210>  17
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  17
tggatttaga gtctctcagc tgg                                               23


<210>  18
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  18
taggcagaca gacttgtcac tgg                                               23


<210>  19
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  19
agctggtaca cggcagggtc agg                                               23


<210>  20
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  20
gctggtacac ggcagggtca ggg                                               23


<210>  21
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  21
tctctcagct ggtacacggc agg                                               23


<210>  22
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  22
tttcaaaacc tgtcagtgat tgg                                               23


<210>  23
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  23
gattaaaccc ggccactttc agg                                               23


<210>  24
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  24
ctcgaccagc ttgacatcac agg                                               23


<210>  25
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  25
agagtctctc agctggtaca cgg                                               23


<210>  26
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  26
ctctcagctg gtacacggca ggg                                               23


<210>  27
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  27
aagttcctgt gatgtcaagc tgg                                               23


<210>  28
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  28
atcctcctcc tgaaagtggc cgg                                               23


<210>  29
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  29
tgctcatgac gctgcggctg tgg                                               23


<210>  30
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  30
acaaaactgt gctagacatg agg                                               23


<210>  31
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  31
atttgtttga gaatcaaaat cgg                                               23


<210>  32
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  32
catcacagga actttctaaa agg                                               23


<210>  33
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  33
gtcgagaaaa gctttgaaac agg                                               23


<210>  34
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  34
ccactttcag gaggaggatt cgg                                               23


<210>  35
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  35
ctgacaggtt ttgaaagttt agg                                               23


<210>  36
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  36
agctttgaaa caggtaagac agg                                               23


<210>  37
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  37
tggaataatg ctgttgttga agg                                               23


<210>  38
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  38
agagcaacag tgctgtggcc tgg                                               23


<210>  39
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  39
ctgtggtcca gctgaggtga ggg                                               23


<210>  40
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  40
ctgcggctgt ggtccagctg agg                                               23


<210>  41
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  41
tgtggtccag ctgaggtgag ggg                                               23


<210>  42
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  42
cttcttcccc agcccaggta agg                                               23


<210>  43
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  43
acacggcagg gtcagggttc tgg                                               23


<210>  44
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  44
cttcaagagc aacagtgctg tgg                                               23


<210>  45
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  45
ctggggaaga aggtgtcttc tgg                                               23


<210>  46
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  46
tcctcctcct gaaagtggcc ggg                                               23


<210>  47
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  47
ttaatctgct catgacgctg cgg                                               23


<210>  48
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  48
acccggccac tttcaggagg agg                                               23


<210>  49
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  49
ttcttcccca gcccaggtaa ggg                                               23


<210>  50
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  50
cttacctggg ctggggaaga agg                                               23


<210>  51
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  51
gacaccttct tccccagccc agg                                               23


<210>  52
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  52
gctgtggtcc agctgaggtg agg                                               23


<210>  53
<211>  23
<212>  DNA
<213>  Homo sapiens

<400>  53
ccgaatcctc ctcctgaaag tgg                                               23


