                               SEQUENCE LISTING

<110> OHIO STATE INNOVATION FOUNDATION
 
<120> ANTI-MIR-21 LIPID NANOPARTICLE COMPOSITIONS, METHODS OF MAKING 
      AND METHODS OF USING THE SAME

<130> 53-56088/OSIF-2014-218

<140>
<141>

<150> 61/975,366
<151> 2014-04-04

<160> 28     

<170> PatentIn version 3.5

<210> 1
<211> 22
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220> 
<221> misc_feature
<222> (1)..(4)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (1)..(22)
<223> 2'O-methylated modified base

<220> 
<221> misc_feature
<222> (19)..(22)
<223> Phosphorothioate linkage

<400> 1
ucaacaucag ucugauaagc ua                                                22


<210> 2
<211> 22
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220> 
<221> misc_feature
<222> (1)..(4)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (1)..(22)
<223> 2'O-methylated modified base

<220> 
<221> misc_feature
<222> (19)..(22)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (22)..(22)
<223> a-CHOL

<400> 2
ucaacaucag ucugauaagc ua                                                22


<210> 3
<211> 22
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220> 
<221> misc_feature
<222> (1)..(4)
<223> Phosphorothioate linkage

<220> 
<221> misc_feature
<222> (19)..(22)
<223> Phosphorothioate linkage

<400> 3
ucaacaucag ucugauaagc ua                                                22


<210> 4
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220> 
<221> misc_feature
<222> (1)..(22)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (1)..(2)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (8)..(8)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (12)..(12)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (16)..(16)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (20)..(22)
<223> Locked nucleic acid

<400> 4
tcaacatcag tctgataagc ta                                                22


<210> 5
<211> 15
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220> 
<221> misc_feature
<222> (1)..(15)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (1)..(1)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (3)..(3)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (6)..(7)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (10)..(10)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (12)..(12)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (14)..(15)
<223> Locked nucleic acid

<400> 5
cagtctgata agcta                                                        15


<210> 6
<211> 8
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220> 
<221> misc_feature
<222> (1)..(8)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (1)..(8)
<223> Locked nucleic acid

<400> 6
gataagct                                                                 8


<210> 7
<211> 16
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220> 
<221> misc_feature
<222> (1)..(16)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (1)..(2)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (6)..(6)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (10)..(10)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (12)..(13)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (15)..(16)
<223> Locked nucleic acid

<400> 7
tcagtctgat aagcta                                                       16


<210> 8
<211> 16
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220> 
<221> misc_feature
<222> (1)..(6)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (1)..(2)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (6)..(6)
<223> Locked nucleic acid

<220> 
<221> misc_feature
<222> (7)..(10)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (10)..(10)
<223> Locked nucleic acid

<220> 
<221> misc_feature
<222> (11)..(12)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (12)..(13)
<223> Locked nucleic acid

<220> 
<221> misc_feature
<222> (14)..(16)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (15)..(16)
<223> Locked nucleic acid

<400> 8
tcagtctgat aagcta                                                       16


<210> 9
<211> 15
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220> 
<221> misc_feature
<222> (1)..(15)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (1)..(2)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (6)..(6)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (10)..(10)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (12)..(12)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (14)..(15)
<223> Locked nucleic acid

<400> 9
tcagtctgat aagct                                                        15


<210> 10
<211> 15
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220> 
<221> misc_feature
<222> (1)..(10)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (1)..(2)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (6)..(6)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (10)..(10)
<223> Locked nucleic acid

<220> 
<221> misc_feature
<222> (11)..(12)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (12)..(12)
<223> Locked nucleic acid

<220> 
<221> misc_feature
<222> (13)..(15)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (14)..(15)
<223> Locked nucleic acid

<400> 10
tcagtctgat aagct                                                        15


<210> 11
<211> 16
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220> 
<221> misc_feature
<222> (1)..(16)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (1)..(2)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (6)..(6)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (10)..(10)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (13)..(13)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (15)..(16)
<223> Locked nucleic acid

<400> 11
tcagtctgat aagcta                                                       16


<210> 12
<211> 16
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220> 
<221> misc_feature
<222> (1)..(10)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (1)..(2)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (6)..(6)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (10)..(10)
<223> Locked nucleic acid

<220> 
<221> misc_feature
<222> (11)..(13)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (13)..(13)
<223> Locked nucleic acid

<220> 
<221> misc_feature
<222> (14)..(16)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (15)..(16)
<223> Locked nucleic acid

<400> 12
tcagtctgat aagcta                                                       16


<210> 13
<211> 16
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220> 
<221> misc_feature
<222> (1)..(10)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (1)..(2)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (6)..(6)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (10)..(10)
<223> Locked nucleic acid

<220> 
<221> misc_feature
<222> (11)..(13)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (13)..(16)
<223> Locked nucleic acid

<220> 
<221> misc_feature
<222> (14)..(16)
<223> Phosphorothioate linkage

<400> 13
tcagtctgat aagcta                                                       16


<210> 14
<211> 16
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220> 
<221> misc_feature
<222> (1)..(16)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (1)..(2)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (6)..(6)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (10)..(10)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (13)..(13)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (15)..(16)
<223> Locked nucleic acid

<400> 14
tcagtctgat aagcta                                                       16


<210> 15
<211> 15
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220> 
<221> misc_feature
<222> (1)..(15)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (1)..(2)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (6)..(6)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (9)..(9)
<223> 2'O-methylated modified base

<220> 
<221> modified_base
<222> (10)..(10)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (11)..(11)
<223> 2'O-methylated modified base

<220> 
<221> modified_base
<222> (12)..(13)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (14)..(14)
<223> 2'O-methylated modified base

<220> 
<221> modified_base
<222> (15)..(15)
<223> Locked nucleic acid

<400> 15
tcagtctgat aagct                                                        15


<210> 16
<211> 16
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Description of Combined DNA/RNA Molecule: Synthetic
      oligonucleotide


<220> 
<221> misc_feature
<222> (1)..(16)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (1)..(2)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (3)..(5)
<223> 2'O-methylated modified base

<220> 
<221> modified_base
<222> (6)..(6)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (7)..(9)
<223> 2'O-methylated modified base

<220> 
<221> modified_base
<222> (10)..(10)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (11)..(11)
<223> 2'O-methylated modified base

<220> 
<221> modified_base
<222> (12)..(13)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (14)..(14)
<223> 2'O-methylated modified base

<220> 
<221> modified_base
<222> (15)..(16)
<223> Locked nucleic acid

<400> 16
tcagucugat aagcta                                                       16


<210> 17
<211> 15
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220> 
<221> misc_feature
<222> (1)..(15)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (1)..(2)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (3)..(5)
<223> 2'O-methylated modified base

<220> 
<221> modified_base
<222> (6)..(6)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (7)..(9)
<223> 2'O-methylated modified base

<220> 
<221> modified_base
<222> (10)..(10)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (11)..(11)
<223> 2'O-methylated modified base

<220> 
<221> modified_base
<222> (12)..(13)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (14)..(14)
<223> 2'O-methylated modified base

<220> 
<221> modified_base
<222> (15)..(15)
<223> Locked nucleic acid

<400> 17
tcagtctgat aagct                                                        15


<210> 18
<211> 16
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220> 
<221> misc_feature
<222> (1)..(6)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (1)..(2)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (5)..(5)
<223> 2'O-methylated modified base

<220> 
<221> modified_base
<222> (6)..(6)
<223> Locked nucleic acid

<220> 
<221> misc_feature
<222> (7)..(10)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (7)..(7)
<223> 2'O-methylated modified base

<220> 
<221> modified_base
<222> (10)..(10)
<223> Locked nucleic acid

<220> 
<221> misc_feature
<222> (11)..(12)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (12)..(13)
<223> Locked nucleic acid

<220> 
<221> misc_feature
<222> (14)..(16)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (14)..(14)
<223> 2'O-methylated modified base

<220> 
<221> modified_base
<222> (15)..(16)
<223> Locked nucleic acid

<400> 18
tcagtctgat aagcta                                                       16


<210> 19
<211> 16
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220> 
<221> misc_feature
<222> (1)..(6)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (1)..(1)
<223> Locked nucleic acid

<220> 
<221> misc_feature
<222> (8)..(11)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (10)..(16)
<223> Locked nucleic acid

<220> 
<221> misc_feature
<222> (13)..(16)
<223> Phosphorothioate linkage

<400> 19
tcagtctgat aagcta                                                       16


<210> 20
<211> 15
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220> 
<221> misc_feature
<222> (1)..(6)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (1)..(1)
<223> Locked nucleic acid

<220> 
<221> misc_feature
<222> (8)..(11)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (9)..(15)
<223> Locked nucleic acid

<220> 
<221> misc_feature
<222> (13)..(15)
<223> Phosphorothioate linkage

<400> 20
tcagtctgat aagct                                                        15


<210> 21
<211> 16
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220> 
<221> misc_feature
<222> (1)..(6)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (1)..(1)
<223> Locked nucleic acid

<220> 
<221> misc_feature
<222> (8)..(10)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (9)..(16)
<223> Locked nucleic acid

<220> 
<221> misc_feature
<222> (13)..(16)
<223> Phosphorothioate linkage

<400> 21
tcagtctgat aagcta                                                       16


<210> 22
<211> 15
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220> 
<221> misc_feature
<222> (1)..(10)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (1)..(2)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (6)..(6)
<223> Locked nucleic acid

<220> 
<221> modified_base
<222> (9)..(10)
<223> Locked nucleic acid

<220> 
<221> misc_feature
<222> (11)..(13)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (13)..(15)
<223> Locked nucleic acid

<220> 
<221> misc_feature
<222> (14)..(15)
<223> Phosphorothioate linkage

<400> 22
tcagtctgat aagct                                                        15


<210> 23
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220> 
<221> misc_feature
<222> (1)..(9)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (1)..(22)
<223> 2'O-methylated modified base

<220> 
<221> misc_feature
<222> (19)..(22)
<223> Phosphorothioate linkage

<400> 23
tcaacatcag tctgataagc ta                                                22


<210> 24
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220> 
<221> misc_feature
<222> (1)..(7)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (1)..(22)
<223> 2'O-methylated modified base

<220> 
<221> misc_feature
<222> (17)..(22)
<223> Phosphorothioate linkage

<400> 24
tcaacatcag tctgataagc ta                                                22


<210> 25
<211> 18
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220> 
<221> misc_feature
<222> (1)..(4)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (1)..(18)
<223> 2'O-methylated modified base

<220> 
<221> misc_feature
<222> (15)..(18)
<223> Phosphorothioate linkage

<400> 25
catcagtctg ataagcta                                                     18


<210> 26
<211> 16
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220> 
<221> misc_feature
<222> (1)..(4)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (1)..(16)
<223> 2'O-methylated modified base

<220> 
<221> misc_feature
<222> (13)..(16)
<223> Phosphorothioate linkage

<400> 26
tcagtctgat aagcta                                                       16


<210> 27
<211> 9
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220> 
<221> misc_feature
<222> (1)..(9)
<223> Phosphorothioate linkage

<220> 
<221> modified_base
<222> (1)..(9)
<223> 2'O-methylated modified base

<400> 27
tgataagct                                                                9


<210> 28
<211> 9
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide


<220> 
<221> modified_base
<222> (1)..(9)
<223> 2'O-methylated modified base

<400> 28
tgataagct                                                                9



