                               SEQUENCE LISTING

<110> LIFECELL CORPORATION

<120> MICROBIAL ENZYMES FOR REDUCTION OF ALPHA-GALACTOSE
      FROM COLLAGEN BASED TISSUE

<130> LIF.1164PCT

<140>
<141>

<150> 61/973,470
<151> 2014-04-01

<160> 8     

<170> PatentIn version 3.5

<210> 1
<211> 1677
<212> DNA
<213> Trichoderma reesei

<400> 1
gacaagcgaa ggccttactg caagaacgag tggcagtcta catcttctac agcggacgga       60

gagattatcg aactgcttgt atcatagctg gaacacacaa tgacccctca ctcgattgac      120

cgggccgcga ggccttccgt ctggagtggc cttgctcttc tcctctctac agcacacgca      180

atcgtgatgc ctgatggagt gactggaaaa gttccaagtc tggggtggaa ctcgtggaat      240

gcctaccact gcgatatcga tgaaagcaag tttctctcgg ccgccgaagt cattgtgagc      300

tctgggcttc tcgacgcagg ctacaattat gtcaatattg atgactgctg gtcgatgaaa      360

gacggtcgtg tggacggcca tattgcagtc aacacaactc gcttccctga cggcattgat      420

gggctggcga agaaggtcca cgacttgggc ttgaagttgg gcatttacag cactgctggg      480

actgcaactt gtgctggcta ccctgccagt cttggctacg aggacgtcga tgccgctgat      540

tttgccgact ggggcgtgga ctatctgaaa tatgacaatt gcaacgtccc ttcagactgg      600

caagatgaat acgtcgcctg cgcccccgac gccgtccaaa ccggccccaa cggcacctgc      660

tcaaccgccc ttgagccaaa cctcgcccct ccgggctacg actggagcac ttccaagtca      720

gccgagcgct tcaacgccat gaggaacgcc ctggcgaagc agagccgcga gatcgtgctc      780

agcctgtgca tctggggagt ggccgacgtc ttctcctggg gcaacgagac aggcatcagc      840

tggcgcatga gcggcgatat ttcgcccgaa tggggctccg tgacgcacat catcaacatg      900

aattcgttca agatgaattc cgtcggcttc tggggccaca acgacgcgga tatactcgag      960

gtcggcaacg gcaacctgac ggctgctgag acgcggacgc actttgcgct gtgggcggcc     1020

atgaagtcgc cgctgctgat cgggacggat cttgctcagt tgtcgcagga gaacattgag     1080

ttgctgaaga ataagcatct gctggcgttt aaccaggaca gcgtctacgg tcagcctgcc     1140

acgccctata aatggggcgt caaccctgac tggaccttta actatacgaa ccctgccgag     1200

tactgggccg gtccatcgtc aaaggggcat ctggtgctga tgatgaacac gctggatcac     1260

acggtgagaa aggaggccaa gtggtctgag attccggggc tgtctgcggg acggtatgag     1320

gtccgggatg tgtggacgga caagagcctt gggtgcctca gctcgtacaa gacggctgtt     1380

gcagctcatg acaccgctgt tattctggtt ggcaagaagt gccgaaactg gtgatggggt     1440

cggaagagga tggatatcga ctggacctct ggttgaatgc ttgtcatcgt ctgcatcctg     1500

acgggagccg gaaggactgt ggctgctgtc tgcttcagat ccagatggaa gcacccttat     1560

ccagactatc cagaaatcac aagccaattg caggattcca gtcttctcag ttactaggag     1620

gtgaataagt aaggtagaga tggctgactt gtcctgctaa aaaaaaaaaa aaaaaaa        1677


<210> 2
<211> 444
<212> PRT
<213> Trichoderma reesei

<400> 2
Met Thr Pro His Ser Ile Asp Arg Ala Ala Arg Pro Ser Val Trp Ser 
1               5                   10                  15      


Gly Leu Ala Leu Leu Leu Ser Thr Ala His Ala Ile Val Met Pro Asp 
            20                  25                  30          


Gly Val Thr Gly Lys Val Pro Ser Leu Gly Trp Asn Ser Trp Asn Ala 
        35                  40                  45              


Tyr His Cys Asp Ile Asp Glu Ser Lys Phe Leu Ser Ala Ala Glu Val 
    50                  55                  60                  


Ile Val Ser Ser Gly Leu Leu Asp Ala Gly Tyr Asn Tyr Val Asn Ile 
65                  70                  75                  80  


Asp Asp Cys Trp Ser Met Lys Asp Gly Arg Val Asp Gly His Ile Ala 
                85                  90                  95      


Val Asn Thr Thr Arg Phe Pro Asp Gly Ile Asp Gly Leu Ala Lys Lys 
            100                 105                 110         


Val His Asp Leu Gly Leu Lys Leu Gly Ile Tyr Ser Thr Ala Gly Thr 
        115                 120                 125             


Ala Thr Cys Ala Gly Tyr Pro Ala Ser Leu Gly Tyr Glu Asp Val Asp 
    130                 135                 140                 


Ala Ala Asp Phe Ala Asp Trp Gly Val Asp Tyr Leu Lys Tyr Asp Asn 
145                 150                 155                 160 


Cys Asn Val Pro Ser Asp Trp Gln Asp Glu Tyr Val Ala Cys Ala Pro 
                165                 170                 175     


Asp Ala Val Gln Thr Gly Pro Asn Gly Thr Cys Ser Thr Ala Leu Glu 
            180                 185                 190         


Pro Asn Leu Ala Pro Pro Gly Tyr Asp Trp Ser Thr Ser Lys Ser Ala 
        195                 200                 205             


Glu Arg Phe Asn Ala Met Arg Asn Ala Leu Ala Lys Gln Ser Arg Glu 
    210                 215                 220                 


Ile Val Leu Ser Leu Cys Ile Trp Gly Val Ala Asp Val Phe Ser Trp 
225                 230                 235                 240 


Gly Asn Glu Thr Gly Ile Ser Trp Arg Met Ser Gly Asp Ile Ser Pro 
                245                 250                 255     


Glu Trp Gly Ser Val Thr His Ile Ile Asn Met Asn Ser Phe Lys Met 
            260                 265                 270         


Asn Ser Val Gly Phe Trp Gly His Asn Asp Ala Asp Ile Leu Glu Val 
        275                 280                 285             


Gly Asn Gly Asn Leu Thr Ala Ala Glu Thr Arg Thr His Phe Ala Leu 
    290                 295                 300                 


Trp Ala Ala Met Lys Ser Pro Leu Leu Ile Gly Thr Asp Leu Ala Gln 
305                 310                 315                 320 


Leu Ser Gln Glu Asn Ile Glu Leu Leu Lys Asn Lys His Leu Leu Ala 
                325                 330                 335     


Phe Asn Gln Asp Ser Val Tyr Gly Gln Pro Ala Thr Pro Tyr Lys Trp 
            340                 345                 350         


Gly Val Asn Pro Asp Trp Thr Phe Asn Tyr Thr Asn Pro Ala Glu Tyr 
        355                 360                 365             


Trp Ala Gly Pro Ser Ser Lys Gly His Leu Val Leu Met Met Asn Thr 
    370                 375                 380                 


Leu Asp His Thr Val Arg Lys Glu Ala Lys Trp Ser Glu Ile Pro Gly 
385                 390                 395                 400 


Leu Ser Ala Gly Arg Tyr Glu Val Arg Asp Val Trp Thr Asp Lys Ser 
                405                 410                 415     


Leu Gly Cys Leu Ser Ser Tyr Lys Thr Ala Val Ala Ala His Asp Thr 
            420                 425                 430         


Ala Val Ile Leu Val Gly Lys Lys Cys Arg Asn Trp 
        435                 440                 


<210> 3
<211> 2438
<212> DNA
<213> Trichoderma reesei

<400> 3
cacagttggc cccggactct gtggtggcca atcttcacga tgctcggcgc tccctctcct       60

agaaggctgg cggacgtcct cgccgtgacc gcgggactgg tggcctctgt tagggcagca      120

agtcccatct ccgtgtctgg caagtcgttt gccctcaacg gcgacaacgt ctcgtaccgc      180

ttccacgtcg acgacgactc taaggacctc atcggcgacc actttggcgg ccctgccacg      240

gaagatggcg tcttcccccc catcatcggc cccatccagg gctgggtcga cctcatcggc      300

cggcagcggc gcgagttccc cgacctgggc cgcggcgact ttcgcacgcc cgcggtgcac      360

atccggcagg cggcgggcta cacggtcagc gacttccagt acaagtcgca ccgcgtcgtc      420

gagggcaagc cggcgctgcg cggcctgccg tcgacgtttg gcgacgccgg cgatgtgtcg      480

actctggtcg tgcacatgta tgataactac agctccgtgg ccgccgacct gacctactcc      540

atcttcccca aatacgacgc cattgtgcgc agcgtcaaca tcaccaacat gggcaagggt      600

aacatcacca ttgagaagct cgccagcttg agcgtcgatc tgccgtatga ggactttgac      660

atgctggagc tcaagggtga ctgggctcgc gagggaaagc ggctgcgtcg caaggttgac      720

tacggctctc agggtttcgg gagcacgact ggctattctt cccatctcca caaccccttc      780

ttctcgctca tcacgcctac gacgaccgag tcccaaggag aggcatgggg cttctccctt      840

gtgtacactg gctccttctc cgtcgaggtc gaaaagggtt cgcagggtct cacgcgagcc      900

gccattggcg tcaaccccta tcaactgtcg tggccgttgg gccctggcga gaccttcagc      960

agccccgagg cggttgccgt cttctctacc actggcgttg gcggaatgtc gcgaaagttc     1020

cacaacctgt accgcaagca tctgatcaag agcaaattcg cgacgcagat gcaccccgtc     1080

ttgctcaaca gctgggaggg cctcggcttc gactacaacg acaccaccat tctgcatctg     1140

gcgcaggagt ctgccgatct cggcatcaag ctgtttgtgc tggatgatgg ctggtttggc     1200

gtcaagcatc ctcgagttag tgacaatgct ggcctgggcg actgggaggc gaaccccaag     1260

aggttcccgc agggcctgcc agacttcatt agcgacgtga caaagctcaa ggtggccaac     1320

tcctctgatc atctgcagtt tggcctctgg ttcgagcctg aaatggtcaa ccccaactca     1380

accctataca tggaacaccc ggactgggcg attcacgccg ggtcgtaccc tcgtaccttg     1440

acgaggaacc agctggtgct caacgtcgct ctcccagagg tgcaggattt catcattgag     1500

tcgctgtcca acattctgag caacgccagc atttcgtacg tcaagtggga caacaaccgc     1560

ggcatccacg aggcccctta ccccgggctc gactacgcct acatgctggg cctgtaccgc     1620

gtctttgaca cgctgtcgtc aaagttcccc aatgttcgct gggagggttg cgcgtctggc     1680

ggcggccgct tcgatcccgg cgtgctgcag tactttcctc acatctggac gtctgacgac     1740

acggatgccg tggagcgcat tgcgatccag tttggcacgt cgctcgtgta tccgccgtcg     1800

gccatgggag cccacgtctc tgccgtaccg aacggccaga cgcagcgcac gacgtcgatt     1860

gccttccgcg cccacgttgc catgatggga ggttcgtttg gcttcgagct cacccctgcg     1920

gagatgccgg aggacgacaa ggcgcagatc ccgggcatca ttgcgctggc ggaaaaggtg     1980

aaccccattg ttgtcaaggg cgacatgtgg cggctgagcc tgccggagga gtccaactgg     2040

ccggcggcgc tcttcatctc gcaggacggc agccaggcgg tgctgttcta cttccagatc     2100

cgggccaaca tcaacaacgc gtggccggtg ctgcggctgc agggcctgga tgcgtcggcc     2160

aagtacaaga ttgacggcaa ccagacgttc tcgggggcga cgctgatgaa catcgggctg     2220

cagtatcagt tcaatgggga ctatgacagc aaggtggtgt ttttggagaa gcagacatga     2280

gctgattttg tgaacccatc accatgacct agatgaccta caggctttgt ttgatgcatc     2340

gtttcttcag tagcacttat gtatggtagc ttagcattca gtagttaata cacagcaggc     2400

gttttctgac tgaaaaaaga aaaaaaaaaa aaaaaaaa                             2438


<210> 4
<211> 746
<212> PRT
<213> Trichoderma reesei

<400> 4
Met Leu Gly Ala Pro Ser Pro Arg Arg Leu Ala Asp Val Leu Ala Val 
1               5                   10                  15      


Thr Ala Gly Leu Val Ala Ser Val Arg Ala Ala Ser Pro Ile Ser Val 
            20                  25                  30          


Ser Gly Lys Ser Phe Ala Leu Asn Gly Asp Asn Val Ser Tyr Arg Phe 
        35                  40                  45              


His Val Asp Asp Asp Ser Lys Asp Leu Ile Gly Asp His Phe Gly Gly 
    50                  55                  60                  


Pro Ala Thr Glu Asp Gly Val Phe Pro Pro Ile Ile Gly Pro Ile Gln 
65                  70                  75                  80  


Gly Trp Val Asp Leu Ile Gly Arg Gln Arg Arg Glu Phe Pro Asp Leu 
                85                  90                  95      


Gly Arg Gly Asp Phe Arg Thr Pro Ala Val His Ile Arg Gln Ala Ala 
            100                 105                 110         


Gly Tyr Thr Val Ser Asp Phe Gln Tyr Lys Ser His Arg Val Val Glu 
        115                 120                 125             


Gly Lys Pro Ala Leu Arg Gly Leu Pro Ser Thr Phe Gly Asp Ala Gly 
    130                 135                 140                 


Asp Val Ser Thr Leu Val Val His Met Tyr Asp Asn Tyr Ser Ser Val 
145                 150                 155                 160 


Ala Ala Asp Leu Thr Tyr Ser Ile Phe Pro Lys Tyr Asp Ala Ile Val 
                165                 170                 175     


Arg Ser Val Asn Ile Thr Asn Met Gly Lys Gly Asn Ile Thr Ile Glu 
            180                 185                 190         


Lys Leu Ala Ser Leu Ser Val Asp Leu Pro Tyr Glu Asp Phe Asp Met 
        195                 200                 205             


Leu Glu Leu Lys Gly Asp Trp Ala Arg Glu Gly Lys Arg Leu Arg Arg 
    210                 215                 220                 


Lys Val Asp Tyr Gly Ser Gln Gly Phe Gly Ser Thr Thr Gly Tyr Ser 
225                 230                 235                 240 


Ser His Leu His Asn Pro Phe Phe Ser Leu Ile Thr Pro Thr Thr Thr 
                245                 250                 255     


Glu Ser Gln Gly Glu Ala Trp Gly Phe Ser Leu Val Tyr Thr Gly Ser 
            260                 265                 270         


Phe Ser Val Glu Val Glu Lys Gly Ser Gln Gly Leu Thr Arg Ala Ala 
        275                 280                 285             


Ile Gly Val Asn Pro Tyr Gln Leu Ser Trp Pro Leu Gly Pro Gly Glu 
    290                 295                 300                 


Thr Phe Ser Ser Pro Glu Ala Val Ala Val Phe Ser Thr Thr Gly Val 
305                 310                 315                 320 


Gly Gly Met Ser Arg Lys Phe His Asn Leu Tyr Arg Lys His Leu Ile 
                325                 330                 335     


Lys Ser Lys Phe Ala Thr Gln Met His Pro Val Leu Leu Asn Ser Trp 
            340                 345                 350         


Glu Gly Leu Gly Phe Asp Tyr Asn Asp Thr Thr Ile Leu His Leu Ala 
        355                 360                 365             


Gln Glu Ser Ala Asp Leu Gly Ile Lys Leu Phe Val Leu Asp Asp Gly 
    370                 375                 380                 


Trp Phe Gly Val Lys His Pro Arg Val Ser Asp Asn Ala Gly Leu Gly 
385                 390                 395                 400 


Asp Trp Glu Ala Asn Pro Lys Arg Phe Pro Gln Gly Leu Pro Asp Phe 
                405                 410                 415     


Ile Ser Asp Val Thr Lys Leu Lys Val Ala Asn Ser Ser Asp His Leu 
            420                 425                 430         


Gln Phe Gly Leu Trp Phe Glu Pro Glu Met Val Asn Pro Asn Ser Thr 
        435                 440                 445             


Leu Tyr Met Glu His Pro Asp Trp Ala Ile His Ala Gly Ser Tyr Pro 
    450                 455                 460                 


Arg Thr Leu Thr Arg Asn Gln Leu Val Leu Asn Val Ala Leu Pro Glu 
465                 470                 475                 480 


Val Gln Asp Phe Ile Ile Glu Ser Leu Ser Asn Ile Leu Ser Asn Ala 
                485                 490                 495     


Ser Ile Ser Tyr Val Lys Trp Asp Asn Asn Arg Gly Ile His Glu Ala 
            500                 505                 510         


Pro Tyr Pro Gly Leu Asp Tyr Ala Tyr Met Leu Gly Leu Tyr Arg Val 
        515                 520                 525             


Phe Asp Thr Leu Ser Ser Lys Phe Pro Asn Val Arg Trp Glu Gly Cys 
    530                 535                 540                 


Ala Ser Gly Gly Gly Arg Phe Asp Pro Gly Val Leu Gln Tyr Phe Pro 
545                 550                 555                 560 


His Ile Trp Thr Ser Asp Asp Thr Asp Ala Val Glu Arg Ile Ala Ile 
                565                 570                 575     


Gln Phe Gly Thr Ser Leu Val Tyr Pro Pro Ser Ala Met Gly Ala His 
            580                 585                 590         


Val Ser Ala Val Pro Asn Gly Gln Thr Gln Arg Thr Thr Ser Ile Ala 
        595                 600                 605             


Phe Arg Ala His Val Ala Met Met Gly Gly Ser Phe Gly Phe Glu Leu 
    610                 615                 620                 


Thr Pro Ala Glu Met Pro Glu Asp Asp Lys Ala Gln Ile Pro Gly Ile 
625                 630                 635                 640 


Ile Ala Leu Ala Glu Lys Val Asn Pro Ile Val Val Lys Gly Asp Met 
                645                 650                 655     


Trp Arg Leu Ser Leu Pro Glu Glu Ser Asn Trp Pro Ala Ala Leu Phe 
            660                 665                 670         


Ile Ser Gln Asp Gly Ser Gln Ala Val Leu Phe Tyr Phe Gln Ile Arg 
        675                 680                 685             


Ala Asn Ile Asn Asn Ala Trp Pro Val Leu Arg Leu Gln Gly Leu Asp 
    690                 695                 700                 


Ala Ser Ala Lys Tyr Lys Ile Asp Gly Asn Gln Thr Phe Ser Gly Ala 
705                 710                 715                 720 


Thr Leu Met Asn Ile Gly Leu Gln Tyr Gln Phe Asn Gly Asp Tyr Asp 
                725                 730                 735     


Ser Lys Val Val Phe Leu Glu Lys Gln Thr 
            740                 745     


<210> 5
<211> 2004
<212> DNA
<213> Trichoderma reesei

<400> 5
gacaagccaa aggcgcaaag cgttcagtta catcgcagca caatgtcgcc cagtgctgca       60

gttctcattc ccctcgcagc ggcagttctg cttcgtcctg tggtcggtca aacgcaatgc      120

ggcggcaatc tgtacactcc ggggacgctc aacttcactc tggagtgcta caatgcgttt      180

caggactgcg tcgctcagtt tgaggccaac gcaagccaag tcgactgcaa cgacggcaag      240

ggaaacctgt tcatgcaaca acaggccaac ttgggggcct cgccaggaag ccagaacaac      300

gacgccatca ttgcctttca ggacattcgc gatctctgtc tgctcagcgg ttcaacaact      360

gcaacgtggg gatatagcga caaccagtgg tattgggcgg ctgccgaaga tgcctgctac      420

acaaacgatc ccacgaggac cgacgttgtc aagactcacc cggcgccgtt ttgcatccag      480

aaccgcgact cttcactgcc tgagtgctac ccacagccgg atgccacccc tcccggcggc      540

ccactcaagg tcatcaagac ggccaagacg cgaaacgggt tcaagtcctc agcccgaggc      600

tggaatacct acggcgtcca agctctggtc aacggttccc aggtcgtgcc gtcctttgct      660

ggacagtcgg gtctgttcta cacccagaag ttcgtcgaga ctcagtgtgg agttcttgcc      720

cgacccgagt tcaagaaggc tgggtacgat ctctgcagcc ttgattcggg ttggcaggct      780

actaccgccg ttgatcagca tggtcgaatc atctacaaca ccacgcgatt caacctcccc      840

gagcttgctt catggctaca caagagggat ttgaagctcg gcgtatacat tacccctggc      900

gtgccatgtc tggctcacaa ccaaaccatc ctcggcacca acatcaagat caaggatgtc      960

ttgaatggga acaacgatca gatcaactgt gactttgact tccgcaaaga tggtgtccag     1020

cagtggcacg attccgtcgt cgcacaatgg gcttcctggg gcgtggacat gctcaaactg     1080

gactttctga cgcctggctc cccttccaat ggcgcaaacc tcgcgtgtga cagttcagat     1140

gctgttcgag cataccagaa ggcaatcaag aagtcaggac gaaaaattcg cctcgacatc     1200

tcgtggaagc tttgccgcaa cgaaacctgg ctacctatct ggagcgacct tgctgagtca     1260

atgcgcactg atcaggatct cgacaactac ggcaccaaca ctttgatggc atggcaggtc     1320

ggccagcgcg cgattgagaa ctacaggcag tacatcggtt tgcaagcgca gagaaatgtc     1380

cccctcacga tctatcctga tatggatgct cttttcacgg tcaaccccga gcatctcgcc     1440

ggtgtaaacg acactattcg ctatacggtt cagaaccact ggcttggagc tggcgccaac     1500

ctcatcattg gtggcgatat ggagcaggtc gatgctctgg ggctcaagct gactaccagc     1560

aagcaatcga ttgatgcggc agactttttt gcaaagtatc ccatgcagcc tcgtaacccc     1620

ggaaccggaa gcaacgccgc caagcagctc caggcctgga tcggtggccc ttcggatgac     1680

cacgaggctt atgtgctcat tgtcaactac gggccagact tggggaatgg tggcttttca     1740

accaagctgt atggaaagca gaaagtgaca gtgtcgttga aggatcttgg tatctctggc     1800

tccgcctgga cttttaccga catatggtcc ggcaagtcca gcagagtgac tgggtcctac     1860

tctgcctggc tcaccgaggg cgagtcccag cttctgcgcc tgaaaaggac tcactagtga     1920

acacgaagac gtatagaggt ttctagagtt tagcgaagca atacacttta taatccctaa     1980

aaaaaaaaaa aaaaaaaaaa aaaa                                            2004


<210> 6
<211> 624
<212> PRT
<213> Trichoderma reesei

<400> 6
Met Ser Pro Ser Ala Ala Val Leu Ile Pro Leu Ala Ala Ala Val Leu 
1               5                   10                  15      


Leu Arg Pro Val Val Gly Gln Thr Gln Cys Gly Gly Asn Leu Tyr Thr 
            20                  25                  30          


Pro Gly Thr Leu Asn Phe Thr Leu Glu Cys Tyr Asn Ala Phe Gln Asp 
        35                  40                  45              


Cys Val Ala Gln Phe Glu Ala Asn Ala Ser Gln Val Asp Cys Asn Asp 
    50                  55                  60                  


Gly Lys Gly Asn Leu Phe Met Gln Gln Gln Ala Asn Leu Gly Ala Ser 
65                  70                  75                  80  


Pro Gly Ser Gln Asn Asn Asp Ala Ile Ile Ala Phe Gln Asp Ile Arg 
                85                  90                  95      


Asp Leu Cys Leu Leu Ser Gly Ser Thr Thr Ala Thr Trp Gly Tyr Ser 
            100                 105                 110         


Asp Asn Gln Trp Tyr Trp Ala Ala Ala Glu Asp Ala Cys Tyr Thr Asn 
        115                 120                 125             


Asp Pro Thr Arg Thr Asp Val Val Lys Thr His Pro Ala Pro Phe Cys 
    130                 135                 140                 


Ile Gln Asn Arg Asp Ser Ser Leu Pro Glu Cys Tyr Pro Gln Pro Asp 
145                 150                 155                 160 


Ala Thr Pro Pro Gly Gly Pro Leu Lys Val Ile Lys Thr Ala Lys Thr 
                165                 170                 175     


Arg Asn Gly Phe Lys Ser Ser Ala Arg Gly Trp Asn Thr Tyr Gly Val 
            180                 185                 190         


Gln Ala Leu Val Asn Gly Ser Gln Val Val Pro Ser Phe Ala Gly Gln 
        195                 200                 205             


Ser Gly Leu Phe Tyr Thr Gln Lys Phe Val Glu Thr Gln Cys Gly Val 
    210                 215                 220                 


Leu Ala Arg Pro Glu Phe Lys Lys Ala Gly Tyr Asp Leu Cys Ser Leu 
225                 230                 235                 240 


Asp Ser Gly Trp Gln Ala Thr Thr Ala Val Asp Gln His Gly Arg Ile 
                245                 250                 255     


Ile Tyr Asn Thr Thr Arg Phe Asn Leu Pro Glu Leu Ala Ser Trp Leu 
            260                 265                 270         


His Lys Arg Asp Leu Lys Leu Gly Val Tyr Ile Thr Pro Gly Val Pro 
        275                 280                 285             


Cys Leu Ala His Asn Gln Thr Ile Leu Gly Thr Asn Ile Lys Ile Lys 
    290                 295                 300                 


Asp Val Leu Asn Gly Asn Asn Asp Gln Ile Asn Cys Asp Phe Asp Phe 
305                 310                 315                 320 


Arg Lys Asp Gly Val Gln Gln Trp His Asp Ser Val Val Ala Gln Trp 
                325                 330                 335     


Ala Ser Trp Gly Val Asp Met Leu Lys Leu Asp Phe Leu Thr Pro Gly 
            340                 345                 350         


Ser Pro Ser Asn Gly Ala Asn Leu Ala Cys Asp Ser Ser Asp Ala Val 
        355                 360                 365             


Arg Ala Tyr Gln Lys Ala Ile Lys Lys Ser Gly Arg Lys Ile Arg Leu 
    370                 375                 380                 


Asp Ile Ser Trp Lys Leu Cys Arg Asn Glu Thr Trp Leu Pro Ile Trp 
385                 390                 395                 400 


Ser Asp Leu Ala Glu Ser Met Arg Thr Asp Gln Asp Leu Asp Asn Tyr 
                405                 410                 415     


Gly Thr Asn Thr Leu Met Ala Trp Gln Val Gly Gln Arg Ala Ile Glu 
            420                 425                 430         


Asn Tyr Arg Gln Tyr Ile Gly Leu Gln Ala Gln Arg Asn Val Pro Leu 
        435                 440                 445             


Thr Ile Tyr Pro Asp Met Asp Ala Leu Phe Thr Val Asn Pro Glu His 
    450                 455                 460                 


Leu Ala Gly Val Asn Asp Thr Ile Arg Tyr Thr Val Gln Asn His Trp 
465                 470                 475                 480 


Leu Gly Ala Gly Ala Asn Leu Ile Ile Gly Gly Asp Met Glu Gln Val 
                485                 490                 495     


Asp Ala Leu Gly Leu Lys Leu Thr Thr Ser Lys Gln Ser Ile Asp Ala 
            500                 505                 510         


Ala Asp Phe Phe Ala Lys Tyr Pro Met Gln Pro Arg Asn Pro Gly Thr 
        515                 520                 525             


Gly Ser Asn Ala Ala Lys Gln Leu Gln Ala Trp Ile Gly Gly Pro Ser 
    530                 535                 540                 


Asp Asp His Glu Ala Tyr Val Leu Ile Val Asn Tyr Gly Pro Asp Leu 
545                 550                 555                 560 


Gly Asn Gly Gly Phe Ser Thr Lys Leu Tyr Gly Lys Gln Lys Val Thr 
                565                 570                 575     


Val Ser Leu Lys Asp Leu Gly Ile Ser Gly Ser Ala Trp Thr Phe Thr 
            580                 585                 590         


Asp Ile Trp Ser Gly Lys Ser Ser Arg Val Thr Gly Ser Tyr Ser Ala 
        595                 600                 605             


Trp Leu Thr Glu Gly Glu Ser Gln Leu Leu Arg Leu Lys Arg Thr His 
    610                 615                 620                 


<210> 7
<211> 8777
<212> DNA
<213> Clostridium cellulolyticum

<400> 7
ctattgatgc actagattgt gccgttttca agaaacttct gttaggtatt atttaaaaat       60

tacccgggta gaactatggt attttagtcc atatatggtt taaattgtta tatctgaatt      120

tgtaaattgg ttaaggaggc aaacatgtta aaaactatga gtatacttct accatgctta      180

ttgatatttt cacttatatt cagtgtacaa atacctttat cagcttcagc agcaaatgtt      240

gaactcctaa agcagttcga catggaacag gtaaaaataa cagatacata ttatgtaaat      300

gcacttaata aagaggttgc ctacttgcag gcaattgatc caaaccgttt gttggtgggt      360

tttaagaaaa cagctggctt atcaacaact tatagctatt atggagggtg ggaaaacaat      420

accctgattc aaggccatac catgggacat tacatgtcgg cacttgctca ggcttataaa      480

aacactaagt ccgacccgac agtaaatgca gatttgaaaa gccgtatcga tttgattata      540

tccgaattgc aggcttgtca gaataaaaac ggcaatggat atttgtttgc aactccggct      600

acccaatttg atgttgttga aggaaaggcg tccggttcaa gctgggtacc gtggtatacc      660

atgcacaaaa tcatgtccgg tcttcttgac atttataaat ttggaggcaa ccaaaccgca      720

ttgacaatag caaccaactt gggaaattgg atttacaaaa gagtaaacgc ttgggattct      780

gcaacacagt caagggtatt gggtgttgag tatggaggaa tgaatgactg tctctatgaa      840

ttgtataagc tgactggtaa tggcaaccat ttaacagcag cacataaatt tgacgaaaat      900

tcactattta acaccatcgc tgcaggcaca aacgttttac ccggaaaaca tgccaataca      960

actatcccga aattcatcgg tgctttgaat cgctacagca ctctaggaac atcagaatca     1020

tcatacttaa aagcggcaca gcagttctgg gccatagttt tgaaagacca tacatatgta     1080

acagggggca acagcgaaga tgagcgtttc agggacgctg gcaaactgga tgcatacagg     1140

gataatgtaa ataatgaaac ttgtaatgta aataatatgc tgaagctgac taaagagctg     1200

ttcaaggcaa cgggcgacgt taaatatgca gattactatg agaatgcatt gataaacgaa     1260

atcatggctt cacagaatcc ggaaaccggg atggctacgt acttcaaggc tatgggaact     1320

ggatatttca aggtattcag ttcccaattc aatcatttct ggtgctgtac gggaacggga     1380

atggagaatt tcacaaagct gaatgacagc ctgtattata ataatggttc cgacctgtat     1440

gtaaacatgt atctgagttc taccctgaac tggagcgaaa agggtctttc actgacacag     1500

caggccaatc tgccattatc agataaagta acctttacta tcaacagtgc ttcttcatca     1560

gaagtgaaaa ttaaattcag gtcaccagca tggattgctg caggacaaaa tattacggtc     1620

aaagttaacg gtactccaat taatgttgac aaggcgaatg gctatcttga cgtcagcaga     1680

gtgtggcaga caggagatac ggttgagttg accctgccca ccgaagtaag ggtatccaga     1740

ctgactgaca gccccaatac ggtagccttt acatatggtc ccgtagtatt gagtgcaggt     1800

cttggaactg aaagcatgac aactcaatca cacggggtcc aggttttaaa agcaacgaaa     1860

aatgtgacta tcaaagagac tattaatatt aataccgccg ccagtcccag cattgacaat     1920

tggcttgcca atataaagaa caatttggtt caaacgcctg ggaagctgga atttacattg     1980

aaaaataccg acgaggataa ccatttggta ttcacacctc attatcaaag atacaaggac     2040

aggtatggta tctacttcaa gctgggaacg tatgagggta aacaacccac ggataatttg     2100

cttgacaatc cggatattga gtcagggaac accacaggat ggactgtgaa tggtgcgggt     2160

acaattgcct cttcaacagt acaaaagcac tcgggaagct atagtctgct gcatacaggc     2220

aggacaggag cctggaacgg gcctattcag aacattacaa caaaagttca gaatggtaac     2280

acgtatactt gttccggctg ggtaatactg gacaacactg ccagtgcccc gataacaatg     2340

actatcagaa aaacggatga caacggaact tcctatgtca atattgccac tgctaccgga     2400

agcaatagtt cctgggttca attgtcaggt aactatacct taaatgttac aggtgcattg     2460

actgacctga gtatatattt tgaaggaccg gacagcggca ccaattttta tgtggatgat     2520

gccttagtta aggtttatgg caaaactacc ttctatcaga atacttcttt tggcggtact     2580

gcggtgtcgc tgaatccagg cagctatact actgctcagc tcactgctgc aggtatttct     2640

gataactggg catcatcaat caaaatacct gaaggctata cggttgagat ttatgatgat     2700

gacaatttca ctggtacaaa gtggtctttt agtgcagata attcgaactt tatagaagcc     2760

ggatgcaatg acaaaatgtc ttccgtgaaa attttcccca ctctgagtca agtgaagtat     2820

ggggatatta acagggacgg tactgtagat actattgact ttgcactttt aaagcagttt     2880

ttgttgggtg ctcaggtcac aattgattcg gtagcggctg atttggacgg cgatgaatct     2940

gtgacggcaa tggattttgc ggtatttaag aagtatctgc tgggacaaat aacagagttg     3000

cctgcttttt gatgttccga tggaattatt tgattccaaa tttattgata tagcaaatca     3060

gccgattcgt tctggtttta gaacagtcta aataacgaaa atttaggggg gataaataga     3120

atgaaaaaag ttcgtacagt cagtacaatt atctttttag gacttatgat taccgtaatt     3180

atggttttga atactggtgc atgggataac ggtcttgcaa aaacaccacc gatgggatgg     3240

aacagttgga acatattcca tggagacatt aatgaaacta aaatcaaaca gattgctgat     3300

accatggtaa gctcgggtat gaaggaagct ggctatgtat acctgaatct ggatgataac     3360

tggatggcaa atcctgcaag agattccaat gggaacttgc gggccgatcc tacacgattc     3420

ccaagtggga ttagggcttt agctgattat gtacatgcaa aaggtctcaa actagggata     3480

tacggatgtc gtggaacaat gacctgtatg aatattcctc aaagcggaag caagggttat     3540

gaggacaagg atgcaaagac atttgcttca tggggaattg attaccttaa atatgataac     3600

tgcaatatac ctaacggaag tgacatgaaa accgattacc agaaaatgca gaccgctctt     3660

gcaaattgcg gaagaccaat agtattcagt atatgtgcat ggggatatca gagctggatg     3720

cctgcaacag gtaatttatg gcgtactacc ggggatttcg ctgataagtg ggataacgga     3780

aacgaatggt tcaaaggtat tataaatgca attgatggta atgcacaata cacaagttca     3840

gccgcacccg gtgcatggaa tgatcctgat atgcttgaaa tcggaaacgg tggatgtaca     3900

acagaggaat accgtacaca gatgagtatg tggagtatga tggcttctcc ccttattgca     3960

ggaaatgata taaggaccat gtcacagaca acaaaggata ttctattgaa taaggaagta     4020

atagcaatag accaggatcc tgcaggagtt cagggaaaaa gagttaaaag tgcaaatggt     4080

cttgagattt gggtaaaacc actgggtacg aatggtacaa ctaaggcagt tgctttattg     4140

aacaggaatt cggcaacatc caatattaca gttaattggt cagatatagg tgtaagtgga     4200

agtgttacgg tcagggattt gtgggctaaa tctgacaaag gcagttttac gggctcatac     4260

acagcgtctg ttccttcaca tggaactgtt ttgattaaga tttccactga gccgccggca     4320

cctgttgatg caacaaagca aatagaagca gagagttata gcaatcagtc aggaatccag     4380

acagaaacct gttcggaagg cggagaggat gtaggcttta tcgaaaacgg ggactatact     4440

gtttacagca ttgtggattt cggcgatgga gtcggaggct tccaggcaag agtagcaagt     4500

gcgaccagcg gaggcaatat tgagattaga cttgacagcc ctgccgggac tttaattgga     4560

acctgtccgg ttgccggaac aggggattgg cagacttata ctgatgtaaa atgtactgtc     4620

agcggggcaa caggaaaaca tgatgtatac cttgtattta aaggagatag cggatattta     4680

ttcaacctta attggttcac atttactcca ggaagtgtca atacgggtac attgggtgat     4740

ttaaattccg acggacaagt agacgcgata gatttacagt tattgaaaaa gtatatttta     4800

ggactgggag caatcgaaaa tacaaaactg gcagatttgg atgccaacgg agatatcaat     4860

gcaatagatt tttcactgct gaaacaattc ttactaggca taaggaccag ctttccgggg     4920

cagggggcag cataatttag cgatatcctg ataggaggtt gaaaatgaaa agaaagattt     4980

tatctgttct tttgcttgtc acaatgacta cagcattgtt ttcagctaca ccgatgaata     5040

ccgcttcagc cgcaagtacg gactttgtac tagacggaaa caatatcaaa gcgggcaaca     5100

tcaacggtct cacatttaag gggttcggcg tcctcagtgg aaacagctca agtgcactgt     5160

tgatggatta caagtcggag catcctgaga aatatacaga attactgcaa attctgttcg     5220

gtggaaaaaa tccgattatg acacatgtca aaattgagat gggtaatgac cgtaacaact     5280

ccaccggacc agatccctca acaatgcgtt gggaaaatga gacggctaat gtcaaaagac     5340

accccggatt tcaacttgcg gctgatgcca agaaggtgaa ccccaatctt aaagtcagca     5400

tattacgctg gaatgccccc ggttgggcaa atagcaatga taagatttat acatggtata     5460

agaacaccat attagcagca taccgtcaat atggttatat gattgattac gtaaacccgg     5520

gggtcaacga acaaacaccg aatttaacct ggactaagca atacgcccag cgtatcaaaa     5580

cagacagtac aggttttaac aatgctgaag aacgggcact ttacaacaat attaaggtgg     5640

tgatttccga cgaagtttcc gtcggttcct tcggggatga tatggtcagt gattccaccc     5700

ttcgtgatgc cgtatctgtc gctgcatatc actataatac tgatgacaat agcttgggaa     5760

gtttcaaaca gcttgccgaa tcctttgaca aagaggtgtg gaatagtgaa gcacaggcca     5820

cttttagtaa ttcgtccttt cgtcccaata acaatatgaa agatccaaca gtagcaggaa     5880

ccggcatagg aggcacaaat gggccactgg aaatgggaaa tactgttata aaggggtttg     5940

taaattcaag gaggacacat tttatctatc agccagtcat tgggtccttc tatgagggtg     6000

ggcagtactc ttttaaggaa ttggtatccg cacgtgatcc ttggtccggg tggattcact     6060

atgatgccgg tcttgtcata cttcggcatt tcagttggtt ttcaaaggct ggctgggaaa     6120

atgagagtaa taccgcaggg gtttggaggg ctgtacctca ggcgagtttc acaggtgcga     6180

cgggtacgaa tcctgttaat gggcgtaatg gcactcccag ttatatgaca ctagcttctc     6240

cggacaagca tgatttttca actatcttca ttaacgatag tgaatattcc aaaacctaca     6300

cgtttaagac catcaatatg gcttattcgg gaaacccctc gctggaagta tgggaaacta     6360

gagcagctga taagggagca tcttttaata gtaattacat gaagtacact ggaacggttt     6420

ccacaaatag cagcggagtt tatacagtaa acgtaaaacc ttattcgatt gttacagtta     6480

caacattaag taacagtgga aaagcagagt tcaatacgcc tcttccggtg gagggggacc     6540

gcccggttct ggatacagac aagacaggtt caatgcagga taccagggat aatatattat     6600

atgctgacaa ctttgattat tccagcaaaa ctgcccctgt catagcggag gggggacaaa     6660

tcacaggaac ccaaagttat atagattccc gaggaggctc aaaaagtgct ataccacgtt     6720

atgccagcga cagaaacggt gcatttgaag tgtatcttcc tgacgggtcc agcaactata     6780

tccttcgcca acaggtgaat cagtcaagta tggggcttgg gggcacatgg aataatggta     6840

atccaatcac cggcattgga gataaccgtt ggatgaacta taaggcaagt gtggatgttt     6900

catttgaaca caacagtaca gagggcggta acaattatgc tgcaatcggt gccagacagc     6960

agggtggtga aaattcacac tacttaaatg gtactcctta tatactgaaa ttctggtttg     7020

acggcggttg gtcgctacta gtaaatggaa gttccgtggc aaatggtaat gtagcaagcg     7080

gctcgggtgg agtgaaaatc agtggtttta atacagctta taatgcatgg cataacatct     7140

ctatcatggt tgcagacaat aaggtgactg cgtatctgga caataccatc ctttatacct     7200

atacagatac tacccaaaga ttgtccgggc gtattgatct ggcaagcggc tattacaata     7260

cttgttttga caatttgaag gttgaaacaa tagacggtta tgcaccttac tactctgaaa     7320

tgctggacaa tctggaaatg tacgatttgt cttctgtttc tgctacaaag cttgtttacg     7380

gcggttcttg ggcacatgaa aacggcaaat ccatgtacaa ttaccaacga tcactttcca     7440

cgagccaggg aataggtgct actattcagt attcattcac tggcaccggg ctggacattc     7500

ttggagccaa caacggttct gctaagttag aggtaactgt tgatggaaga gttgttaatt     7560

cctcagtggg aaccatggtt tcagggaatt tacaccaaaa ctttacgctt cacggtcttg     7620

agtacggtaa gcatacggtt tgtttgaagg tgttaagtgg tactatggtt gtcgatgctg     7680

ttggggttgt tgcaaacata gccggtgctt cggagatacc cgttgaacaa tctgcgtatt     7740

caaggataga agcagagagc tacagcaacc agtcaggaat ccagacagaa acctgttcgg     7800

aaggcggaga ggatgtgggc tttgttgaaa acggcgacta tactgtttac aacaatgtgg     7860

atttcggcga tggtgtcgga ggcttccaag caagagtagc aagtgcaacc agtggaggca     7920

atattgagat caggcttgac agctctaccg ggactttgat aggaacttgt cctgttgccg     7980

gaacagggga ttggcagact tatactgatg caaaatgtac tgtcagcggg gtaacaggaa     8040

aacatgatgt ataccttgta tttaaaggag atagcggata tttatttaat cttaactggt     8100

ttacatttag tgagaaaact gtcataggga atttgggtga tataaactcg gacggacaag     8160

tagatgcaat agatttacag gtattgaaaa agtatctttt gcaactaggg gaaattggag     8220

atacgaagct ggcagatttg gatgccaacg gagaaattaa cgcaatcgat ttttcattac     8280

tcaaacaatt tttactgggt actattatta gttttccggg agaagcacta taaggggaca     8340

tcaccgttcc atatgtgtga agaattacaa atataatata aggaggcttt tttggtatgt     8400

tgagaaagaa aattttatgt atatttcttg tgactgtatt aatgctaact atattaccaa     8460

tacctcaaca gacggtaatg gctgatacag gggtacttaa ggaacttaaa ggaactgaca     8520

tctacaacgg attaagggga ctgaatttta acgagggttg gaaattcaat aagggggatg     8580

taagcaacgg ccagagtacc gggtataatg acagcggctg gtcaggtgtt acactgccac     8640

atgactggag tatttataac acttttaata aatcctcagc agcgggtgca ggaggaggtt     8700

atctggatgg aggaatcgga tggtacagga aaacctttac cgtaccttcg gattatacag     8760

gaaagaaggt attcatt                                                    8777


<210> 8
<211> 604
<212> PRT
<213> Clostridium cellulolyticum

<400> 8
Met Lys Lys Val Arg Thr Val Ser Thr Ile Ile Phe Leu Gly Leu Met 
1               5                   10                  15      


Ile Thr Val Ile Met Val Leu Asn Thr Gly Ala Trp Asp Asn Gly Leu 
            20                  25                  30          


Ala Lys Thr Pro Pro Met Gly Trp Asn Ser Trp Asn Ile Phe His Gly 
        35                  40                  45              


Asp Ile Asn Glu Thr Lys Ile Lys Gln Ile Ala Asp Thr Met Val Ser 
    50                  55                  60                  


Ser Gly Met Lys Glu Ala Gly Tyr Val Tyr Leu Asn Leu Asp Asp Asn 
65                  70                  75                  80  


Trp Met Ala Asn Pro Ala Arg Asp Ser Asn Gly Asn Leu Arg Ala Asp 
                85                  90                  95      


Pro Thr Arg Phe Pro Ser Gly Ile Arg Ala Leu Ala Asp Tyr Val His 
            100                 105                 110         


Ala Lys Gly Leu Lys Leu Gly Ile Tyr Gly Cys Arg Gly Thr Met Thr 
        115                 120                 125             


Cys Met Asn Ile Pro Gln Ser Gly Ser Lys Gly Tyr Glu Asp Lys Asp 
    130                 135                 140                 


Ala Lys Thr Phe Ala Ser Trp Gly Ile Asp Tyr Leu Lys Tyr Asp Asn 
145                 150                 155                 160 


Cys Asn Ile Pro Asn Gly Ser Asp Met Lys Thr Asp Tyr Gln Lys Met 
                165                 170                 175     


Gln Thr Ala Leu Ala Asn Cys Gly Arg Pro Ile Val Phe Ser Ile Cys 
            180                 185                 190         


Ala Trp Gly Tyr Gln Ser Trp Met Pro Ala Thr Gly Asn Leu Trp Arg 
        195                 200                 205             


Thr Thr Gly Asp Ile Ala Asp Lys Trp Asp Asn Gly Asn Glu Trp Phe 
    210                 215                 220                 


Lys Gly Ile Ile Asn Ala Ile Asp Gly Asn Ala Gln Tyr Thr Ser Ser 
225                 230                 235                 240 


Ala Ala Pro Gly Ala Trp Asn Asp Pro Asp Met Leu Glu Ile Gly Asn 
                245                 250                 255     


Gly Gly Cys Thr Thr Glu Glu Tyr Arg Thr Gln Met Ser Met Trp Ser 
            260                 265                 270         


Met Met Ala Ser Pro Leu Ile Ala Gly Asn Asp Ile Arg Thr Met Ser 
        275                 280                 285             


Gln Thr Thr Lys Asp Ile Leu Leu Asn Lys Glu Val Ile Ala Ile Asp 
    290                 295                 300                 


Gln Asp Pro Ala Gly Val Gln Gly Lys Arg Val Lys Ser Ala Asn Gly 
305                 310                 315                 320 


Leu Glu Ile Trp Val Lys Pro Leu Gly Thr Asn Gly Thr Thr Lys Ala 
                325                 330                 335     


Val Ala Leu Leu Asn Arg Asn Ser Ala Thr Ser Asn Ile Thr Val Asn 
            340                 345                 350         


Trp Ser Asp Ile Gly Val Ser Gly Ser Val Thr Val Arg Asp Leu Trp 
        355                 360                 365             


Ala Lys Ser Asp Lys Gly Ser Phe Thr Gly Ser Tyr Thr Ala Ser Val 
    370                 375                 380                 


Pro Ser His Gly Thr Val Leu Ile Lys Ile Ser Thr Glu Pro Pro Ala 
385                 390                 395                 400 


Pro Val Asp Ala Thr Lys Gln Ile Glu Ala Glu Ser Tyr Ser Asn Gln 
                405                 410                 415     


Ser Gly Ile Gln Thr Glu Thr Cys Ser Glu Gly Gly Glu Asp Val Gly 
            420                 425                 430         


Phe Ile Glu Asn Gly Asp Tyr Thr Val Tyr Ser Asn Val Asp Phe Gly 
        435                 440                 445             


Asp Gly Val Gly Gly Phe Gln Ala Arg Val Ala Ser Ala Thr Ser Gly 
    450                 455                 460                 


Gly Asn Ile Glu Ile Arg Leu Asp Ser Pro Ala Gly Thr Leu Ile Gly 
465                 470                 475                 480 


Thr Cys Pro Val Ala Gly Thr Gly Asp Trp Gln Thr Tyr Thr Asp Val 
                485                 490                 495     


Lys Cys Thr Val Ser Gly Ala Thr Gly Lys His Asp Val Tyr Leu Val 
            500                 505                 510         


Phe Lys Gly Asp Ser Gly Tyr Leu Phe Asn Leu Asn Trp Phe Thr Phe 
        515                 520                 525             


Thr Pro Gly Ser Val Asn Thr Gly Thr Leu Gly Asp Leu Asn Ser Asp 
    530                 535                 540                 


Gly Gln Val Asp Ala Ile Asp Leu Gln Leu Leu Lys Lys Tyr Ile Leu 
545                 550                 555                 560 


Gly Leu Gly Ala Ile Glu Asn Thr Lys Leu Ala Asp Leu Asp Ala Asn 
                565                 570                 575     


Gly Asp Ile Asn Ala Ile Asp Phe Ser Leu Leu Lys Gln Phe Leu Leu 
            580                 585                 590         


Gly Ile Arg Thr Ser Phe Pro Gly Gln Gly Ala Ala 
        595                 600                 


