               SEQUENCE LISTING

<110> DSM IP Assets B.V.

<120> Proline-specific endoprotease

<130> 30117-WO-PCT

<160> 17

<170> BiSSAP 1.2

<210> 1
<211> 1565
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..1565
<223> /organism="Artificial Sequence"
      /note="Codon optimized for expression in A. niger"
      /mol_type="unassigned DNA"

<400> 1
atggtcaagt ccatcctggc ctccgtcttc ttcgccgcca ctgctctggc tgctcgcccc      60

cgcttggttc ccaagcccgt ctctcgtccc gccagcagca agtcggctgc caccaccggt     120

gaagcctact tcgagcagct ccttgaccac cacgactctt ccaagggcac cttctcccag     180

cgttactggt ggagcactga gtactggggt ggtcctggaa gccctgttgt cctcttcact     240

cccggtgagg cctccgccga tggctacgag ggatacctga ccaacaacac cctgaccggt     300

ctgtacgctc aggagatcca gggtgccgtc atcttgattg agcaccgtta ctggggcgat     360

tcttctccct acgaggagct gaccgctgaa accctccagt acctcaccct ggagcagtcc     420

attttggatc tgacccactt cgctgagact gtccagcttg agttcgacac cagcaactcc     480

tccaacgccc ccaaggcccc ctgggttctc gtcggtggaa gctactctgg tgctcttgct     540

gcctggactg ctgctgttgc tcctggaacc ttctgggcct accacgccac ctcggctcct     600

gtgcaggcca ttgatgactt ctggcagtac ttcgacccca tccgtcacgg aatggctccc     660

aactgctctc gtgatgtctc cctcgtcgcc aaccacatcg acaccgtcgg caagaacggc     720

tctgctgccg accagcttgc tctgaaggag ctgttcggtc ttgaggctct cgaacactac     780

gatgacttcg ctgctgccct tcccactggt ccctacctct ggcagtccaa caccttcgtc     840

accggctact ccaacttctt cgctttctgc gatgccgttg agaacgtcga ggctggtgct     900

gctgtggtgc ctggccccga gggtgttggt ctgcagaagg ccttgactgg ctacgccaac     960

tggttcaact cgaccatcat ccctggctac tgcgcttcct acggctactg gactgacaac    1020

cgcaccgttg cttgcttcga cacccacaac cccagctctg ccatcttcac cgacacctcc    1080

gtcgataacg ccgtcgaccg ccagtggcag tggttcctct gcaacgagcc cttcttctgg    1140

tggcaggatg gtgctcctga gggtgttcct accattgtgc ctcgcaccat caacgccgaa    1200

tactggcagc gccagtgctc tctctacttc cccgaagtca acggctacac ctacggctcc    1260

gccaagggca agactgctgc cactgtcaac acctggactg gtggctggtc cgacagcaag    1320

aacaccagcc gtctcctctg ggtgaacggc cagtacgacc cctggcgtga ctccggtgtc    1380

tcctccaccc accgccctgg tggtcctctg accagcactg ccgatgagcc cgtgcaggtc    1440

atccccggtg gtttccactg ctcggacctc tacctcaagg actacttcgc caacgccggt    1500

gtcaagcagg tcgtcgacaa cgccgttgct cagatcaagt cctgggttgc tgaatactac    1560

aaata                                                                1565


<210> 2
<211> 521
<212> PRT
<213> Artificial Sequence

<220> 
<223> Proline-specific endoprotease BC2G077

<400> 2
Met Val Lys Ser Ile Leu Ala Ser Val Phe Phe Ala Ala Thr Ala Leu 
1               5                   10                  15      
Ala Ala Arg Pro Arg Leu Val Pro Lys Pro Val Ser Arg Pro Ala Ser 
            20                  25                  30          
Ser Lys Ser Ala Ala Thr Thr Gly Glu Ala Tyr Phe Glu Gln Leu Leu 
        35                  40                  45              
Asp His His Asp Ser Ser Lys Gly Thr Phe Ser Gln Arg Tyr Trp Trp 
    50                  55                  60                  
Ser Thr Glu Tyr Trp Gly Gly Pro Gly Ser Pro Val Val Leu Phe Thr 
65                  70                  75                  80  
Pro Gly Glu Ala Ser Ala Asp Gly Tyr Glu Gly Tyr Leu Thr Asn Asn 
                85                  90                  95      
Thr Leu Thr Gly Leu Tyr Ala Gln Glu Ile Gln Gly Ala Val Ile Leu 
            100                 105                 110         
Ile Glu His Arg Tyr Trp Gly Asp Ser Ser Pro Tyr Glu Glu Leu Thr 
        115                 120                 125             
Ala Glu Thr Leu Gln Tyr Leu Thr Leu Glu Gln Ser Ile Leu Asp Leu 
    130                 135                 140                 
Thr His Phe Ala Glu Thr Val Gln Leu Glu Phe Asp Thr Ser Asn Ser 
145                 150                 155                 160 
Ser Asn Ala Pro Lys Ala Pro Trp Val Leu Val Gly Gly Ser Tyr Ser 
                165                 170                 175     
Gly Ala Leu Ala Ala Trp Thr Ala Ala Val Ala Pro Gly Thr Phe Trp 
            180                 185                 190         
Ala Tyr His Ala Thr Ser Ala Pro Val Gln Ala Ile Asp Asp Phe Trp 
        195                 200                 205             
Gln Tyr Phe Asp Pro Ile Arg His Gly Met Ala Pro Asn Cys Ser Arg 
    210                 215                 220                 
Asp Val Ser Leu Val Ala Asn His Ile Asp Thr Val Gly Lys Asn Gly 
225                 230                 235                 240 
Ser Ala Ala Asp Gln Leu Ala Leu Lys Glu Leu Phe Gly Leu Glu Ala 
                245                 250                 255     
Leu Glu His Tyr Asp Asp Phe Ala Ala Ala Leu Pro Thr Gly Pro Tyr 
            260                 265                 270         
Leu Trp Gln Ser Asn Thr Phe Val Thr Gly Tyr Ser Asn Phe Phe Ala 
        275                 280                 285             
Phe Cys Asp Ala Val Glu Asn Val Glu Ala Gly Ala Ala Val Val Pro 
    290                 295                 300                 
Gly Pro Glu Gly Val Gly Leu Gln Lys Ala Leu Thr Gly Tyr Ala Asn 
305                 310                 315                 320 
Trp Phe Asn Ser Thr Ile Ile Pro Gly Tyr Cys Ala Ser Tyr Gly Tyr 
                325                 330                 335     
Trp Thr Asp Asn Arg Thr Val Ala Cys Phe Asp Thr His Asn Pro Ser 
            340                 345                 350         
Ser Ala Ile Phe Thr Asp Thr Ser Val Asp Asn Ala Val Asp Arg Gln 
        355                 360                 365             
Trp Gln Trp Phe Leu Cys Asn Glu Pro Phe Phe Trp Trp Gln Asp Gly 
    370                 375                 380                 
Ala Pro Glu Gly Val Pro Thr Ile Val Pro Arg Thr Ile Asn Ala Glu 
385                 390                 395                 400 
Tyr Trp Gln Arg Gln Cys Ser Leu Tyr Phe Pro Glu Val Asn Gly Tyr 
                405                 410                 415     
Thr Tyr Gly Ser Ala Lys Gly Lys Thr Ala Ala Thr Val Asn Thr Trp 
            420                 425                 430         
Thr Gly Gly Trp Ser Asp Ser Lys Asn Thr Ser Arg Leu Leu Trp Val 
        435                 440                 445             
Asn Gly Gln Tyr Asp Pro Trp Arg Asp Ser Gly Val Ser Ser Thr His 
    450                 455                 460                 
Arg Pro Gly Gly Pro Leu Thr Ser Thr Ala Asp Glu Pro Val Gln Val 
465                 470                 475                 480 
Ile Pro Gly Gly Phe His Cys Ser Asp Leu Tyr Leu Lys Asp Tyr Phe 
                485                 490                 495     
Ala Asn Ala Gly Val Lys Gln Val Val Asp Asn Ala Val Ala Gln Ile 
            500                 505                 510         
Lys Ser Trp Val Ala Glu Tyr Tyr Lys 
        515                 520     

<210> 3
<211> 104
<212> PRT
<213> Artificial Sequence

<220> 
<223> Amino acid sequence horse heart cytochrome C

<400> 3
Gly Asp Val Glu Lys Gly Lys Lys Ile Phe Val Gln Lys Cys Ala Gln 
1               5                   10                  15      
Cys His Thr Val Glu Lys Gly Gly Lys His Lys Thr Gly Pro Asn Leu 
            20                  25                  30          
His Gly Leu Phe Gly Arg Lys Thr Gly Gln Ala Pro Gly Phe Thr Tyr 
        35                  40                  45              
Thr Asp Ala Asn Lys Asn Lys Gly Ile Thr Trp Lys Glu Glu Thr Leu 
    50                  55                  60                  
Met Glu Tyr Leu Glu Asn Pro Lys Lys Tyr Ile Pro Gly Thr Lys Met 
65                  70                  75                  80  
Ile Phe Ala Gly Ile Lys Lys Lys Thr Glu Arg Glu Asp Leu Ile Ala 
                85                  90                  95      
Tyr Leu Lys Lys Ala Thr Asn Glu 
            100                 

<210> 4
<211> 30
<212> PRT
<213> Artificial Sequence

<220> 
<223> Fragment of cytochrome C digested with PEP BC2G077

<400> 4
Gly Asp Val Glu Lys Gly Lys Lys Ile Phe Val Gln Lys Cys Ala Gln 
1               5                   10                  15      
Cys His Thr Val Glu Lys Gly Gly Lys His Lys Thr Gly Pro 
            20                  25                  30  

<210> 5
<211> 14
<212> PRT
<213> Artificial Sequence

<220> 
<223> Fragment of cytochrome C digested with PEP BC2G077

<400> 5
Asn Leu His Gly Leu Phe Gly Arg Lys Thr Gly Gln Ala Pro 
1               5                   10                  

<210> 6
<211> 32
<212> PRT
<213> Artificial Sequence

<220> 
<223> Fragment of cytochrome C digested with PEP BC2G077

<400> 6
Gly Phe Thr Tyr Thr Asp Ala Asn Lys Asn Lys Gly Ile Thr Trp Lys 
1               5                   10                  15      
Glu Glu Thr Leu Met Glu Tyr Leu Glu Asn Pro Lys Lys Tyr Ile Pro 
            20                  25                  30          



<210> 7
<211> 7
<212> PRT
<213> Artificial Sequence

<220> 
<223> Fragment of cytochrome C digested with PEP BC2G077

<400> 7
Gly Thr Lys Met Ile Phe Ala 
1               5           

<210> 8
<211> 21
<212> PRT
<213> Artificial Sequence

<220> 
<223> Fragment of cytochrome C digested with PEP BC2G077

<400> 8
Gly Ile Lys Lys Lys Thr Glu Arg Glu Asp Leu Ile Ala Tyr Leu Lys 
1               5                   10                  15      
Lys Ala Thr Asn Glu 
            20      

<210> 9
<211> 527
<212> PRT
<213> Aspergillus flavus BC2G101:Afl0942_30CG_Aspergillus_flavus_NRRL3357 527aa


<400> 9
Met Gln Phe Leu Pro Pro Leu Ser Ile Val Thr Leu Leu Ala Ser Trp 
1               5                   10                  15      
Pro Ser Leu Ser Arg Ala Ile His Pro Pro Arg Pro Val Pro Pro Pro 
            20                  25                  30          
Val Ser Arg Pro Val Ser Thr Gln Ser Ser Val Val Glu Gly Asn Ala 
        35                  40                  45              
Thr Phe Glu Gln Leu Leu Asp His His Asp Ser Ser Lys Gly Thr Phe 
    50                  55                  60                  
Ser Gln Arg Tyr Trp Trp Ser Thr Glu Tyr Trp Gly Gly Pro Gly Ser 
65                  70                  75                  80  
Pro Val Val Leu Phe Thr Pro Gly Glu Ala Ser Ala Asp Gly Tyr Glu 
                85                  90                  95      
Gly Tyr Leu Thr Asn Asn Thr Leu Thr Gly Leu Tyr Ala Gln Glu Ile 
            100                 105                 110         
Gln Gly Ala Val Ile Leu Ile Glu His Arg Tyr Trp Gly Asp Ser Ser 
        115                 120                 125             
Pro Tyr Glu Glu Leu Thr Ala Glu Thr Leu Gln Tyr Leu Thr Leu Glu 
    130                 135                 140                 
Gln Ser Ile Leu Asp Leu Thr His Phe Ala Glu Thr Val Gln Leu Glu 
145                 150                 155                 160 
Phe Asp Thr Ser Asn Ser Ser Asn Ala Pro Lys Ala Pro Trp Val Leu 
                165                 170                 175     
Val Gly Gly Ser Tyr Ser Gly Ala Leu Ala Ala Trp Thr Ala Ala Val 
            180                 185                 190         
Ala Pro Glu Thr Phe Trp Ala Tyr His Ala Thr Ser Ala Pro Val Gln 
        195                 200                 205             
Ala Ile Asp Asp Phe Trp Gln Tyr Phe Asp Pro Ile Arg His Gly Met 
    210                 215                 220                 
Ala Pro Asn Cys Ser Arg Asp Val Ser Leu Val Ala Asn His Ile Asp 
225                 230                 235                 240 
Thr Val Gly Lys Asn Gly Ser Ala Ala Asp Gln Leu Ala Leu Lys Glu 
                245                 250                 255     
Leu Phe Gly Leu Glu Ala Leu Glu His Tyr Asp Asp Phe Ala Ala Ala 
            260                 265                 270         
Leu Pro Thr Gly Pro Tyr Leu Trp Gln Ser Asn Thr Phe Val Thr Gly 
        275                 280                 285             
Tyr Ser Asn Phe Phe Ala Phe Cys Asp Ala Val Glu Asn Val Glu Ala 
    290                 295                 300                 
Gly Ala Ala Val Val Pro Gly Pro Glu Gly Val Gly Leu Gln Lys Ala 
305                 310                 315                 320 
Leu Thr Gly Tyr Ala Asn Trp Phe Asn Ser Thr Ile Ile Pro Gly Tyr 
                325                 330                 335     
Cys Ala Ser Tyr Gly Tyr Trp Thr Asp Asn Arg Thr Val Ala Cys Phe 
            340                 345                 350         
Asp Thr His Asn Pro Ser Ser Ala Ile Phe Thr Asp Thr Ser Val Asp 
        355                 360                 365             
Asn Ala Val Asp Arg Gln Trp Gln Trp Phe Leu Cys Asn Glu Pro Phe 
    370                 375                 380                 
Phe Trp Trp Gln Asp Gly Ala Pro Glu Gly Val Pro Thr Ile Val Pro 
385                 390                 395                 400 
Arg Thr Ile Asn Ala Glu Tyr Trp Gln Arg Gln Cys Ser Leu Tyr Phe 
                405                 410                 415     
Pro Glu Val Asn Gly Tyr Thr Tyr Gly Ser Ala Lys Gly Lys Thr Ala 
            420                 425                 430         
Ala Thr Val Asn Thr Trp Thr Gly Gly Trp Ser Asp Ser Lys Asn Thr 
        435                 440                 445             
Ser Arg Leu Leu Trp Val Asn Gly Gln Tyr Asp Pro Trp Arg Asp Ser 
    450                 455                 460                 
Gly Val Ser Ser Thr His Arg Pro Gly Gly Pro Leu Thr Ser Thr Ala 
465                 470                 475                 480 
Asp Glu Pro Val Gln Val Ile Pro Gly Gly Phe His Cys Ser Asp Leu 
                485                 490                 495     
Tyr Leu Lys Asp Tyr Phe Ala Asn Ala Gly Val Lys Gln Val Val Asp 
            500                 505                 510         
Asn Ala Val Ala Gln Ile Lys Ser Trp Val Ala Glu Tyr Tyr Lys 
        515                 520                 525         

<210> 10
<211> 522
<212> PRT
<213> Artificial sequence

<220> 
<223> BC2Gl02:PmeAss_AflProSeq_Mat_Afl0942_30CG

<400> 10
Met Val Lys Ser Ile Leu Ala Ser Val Phe Phe Ala Ala Thr Ala Leu 
1               5                   10                  15      
Ala Ile His Pro Pro Arg Pro Val Pro Pro Pro Val Ser Arg Pro Val 
            20                  25                  30          
Ser Thr Gln Ser Ser Val Val Glu Gly Asn Ala Thr Phe Glu Gln Leu 
        35                  40                  45              
Leu Asp His His Asp Ser Ser Lys Gly Thr Phe Ser Gln Arg Tyr Trp 
    50                  55                  60                  
Trp Ser Thr Glu Tyr Trp Gly Gly Pro Gly Ser Pro Val Val Leu Phe 
65                  70                  75                  80  
Thr Pro Gly Glu Ala Ser Ala Asp Gly Tyr Glu Gly Tyr Leu Thr Asn 
                85                  90                  95      
Asn Thr Leu Thr Gly Leu Tyr Ala Gln Glu Ile Gln Gly Ala Val Ile 
            100                 105                 110         
Leu Ile Glu His Arg Tyr Trp Gly Asp Ser Ser Pro Tyr Glu Glu Leu 
        115                 120                 125             
Thr Ala Glu Thr Leu Gln Tyr Leu Thr Leu Glu Gln Ser Ile Leu Asp 
    130                 135                 140                 
Leu Thr His Phe Ala Glu Thr Val Gln Leu Glu Phe Asp Thr Ser Asn 
145                 150                 155                 160 
Ser Ser Asn Ala Pro Lys Ala Pro Trp Val Leu Val Gly Gly Ser Tyr 
                165                 170                 175     
Ser Gly Ala Leu Ala Ala Trp Thr Ala Ala Val Ala Pro Glu Thr Phe 
            180                 185                 190         
Trp Ala Tyr His Ala Thr Ser Ala Pro Val Gln Ala Ile Asp Asp Phe 
        195                 200                 205             
Trp Gln Tyr Phe Asp Pro Ile Arg His Gly Met Ala Pro Asn Cys Ser 
    210                 215                 220                 
Arg Asp Val Ser Leu Val Ala Asn His Ile Asp Thr Val Gly Lys Asn 
225                 230                 235                 240 
Gly Ser Ala Ala Asp Gln Leu Ala Leu Lys Glu Leu Phe Gly Leu Glu 
                245                 250                 255     
Ala Leu Glu His Tyr Asp Asp Phe Ala Ala Ala Leu Pro Thr Gly Pro 
            260                 265                 270         
Tyr Leu Trp Gln Ser Asn Thr Phe Val Thr Gly Tyr Ser Asn Phe Phe 
        275                 280                 285             
Ala Phe Cys Asp Ala Val Glu Asn Val Glu Ala Gly Ala Ala Val Val 
    290                 295                 300                 
Pro Gly Pro Glu Gly Val Gly Leu Gln Lys Ala Leu Thr Gly Tyr Ala 
305                 310                 315                 320 
Asn Trp Phe Asn Ser Thr Ile Ile Pro Gly Tyr Cys Ala Ser Tyr Gly 
                325                 330                 335     
Tyr Trp Thr Asp Asn Arg Thr Val Ala Cys Phe Asp Thr His Asn Pro 
            340                 345                 350         
Ser Ser Ala Ile Phe Thr Asp Thr Ser Val Asp Asn Ala Val Asp Arg 
        355                 360                 365             
Gln Trp Gln Trp Phe Leu Cys Asn Glu Pro Phe Phe Trp Trp Gln Asp 
    370                 375                 380                 
Gly Ala Pro Glu Gly Val Pro Thr Ile Val Pro Arg Thr Ile Asn Ala 
385                 390                 395                 400 
Glu Tyr Trp Gln Arg Gln Cys Ser Leu Tyr Phe Pro Glu Val Asn Gly 
                405                 410                 415     
Tyr Thr Tyr Gly Ser Ala Lys Gly Lys Thr Ala Ala Thr Val Asn Thr 
            420                 425                 430         
Trp Thr Gly Gly Trp Ser Asp Ser Lys Asn Thr Ser Arg Leu Leu Trp 
        435                 440                 445             
Val Asn Gly Gln Tyr Asp Pro Trp Arg Asp Ser Gly Val Ser Ser Thr 
    450                 455                 460                 
His Arg Pro Gly Gly Pro Leu Thr Ser Thr Ala Asp Glu Pro Val Gln 
465                 470                 475                 480 
Val Ile Pro Gly Gly Phe His Cys Ser Asp Leu Tyr Leu Lys Asp Tyr 
                485                 490                 495     
Phe Ala Asn Ala Gly Val Lys Gln Val Val Asp Asn Ala Val Ala Gln 
            500                 505                 510         
Ile Lys Ser Trp Val Ala Glu Tyr Tyr Lys 
        515                 520         

<210> 11
<211> 521
<212> PRT
<213> Artificial sequence

<220> 
<223> BC2Gl03:PmeAss_AnProSeq_Mat_Afl0942_30CG

<400> 11
Met Val Lys Ser Ile Leu Ala Ser Val Phe Phe Ala Ala Thr Ala Leu 
1               5                   10                  15      
Ala Ala Arg Pro Arg Leu Val Pro Lys Pro Val Ser Arg Pro Ala Ser 
            20                  25                  30          
Ser Lys Ser Ala Val Val Glu Gly Asn Ala Thr Phe Glu Gln Leu Leu 
        35                  40                  45              
Asp His His Asp Ser Ser Lys Gly Thr Phe Ser Gln Arg Tyr Trp Trp 
    50                  55                  60                  
Ser Thr Glu Tyr Trp Gly Gly Pro Gly Ser Pro Val Val Leu Phe Thr 
65                  70                  75                  80  
Pro Gly Glu Ala Ser Ala Asp Gly Tyr Glu Gly Tyr Leu Thr Asn Asn 
                85                  90                  95      
Thr Leu Thr Gly Leu Tyr Ala Gln Glu Ile Gln Gly Ala Val Ile Leu 
            100                 105                 110         
Ile Glu His Arg Tyr Trp Gly Asp Ser Ser Pro Tyr Glu Glu Leu Thr 
        115                 120                 125             
Ala Glu Thr Leu Gln Tyr Leu Thr Leu Glu Gln Ser Ile Leu Asp Leu 
    130                 135                 140                 
Thr His Phe Ala Glu Thr Val Gln Leu Glu Phe Asp Thr Ser Asn Ser 
145                 150                 155                 160 
Ser Asn Ala Pro Lys Ala Pro Trp Val Leu Val Gly Gly Ser Tyr Ser 
                165                 170                 175     
Gly Ala Leu Ala Ala Trp Thr Ala Ala Val Ala Pro Glu Thr Phe Trp 
            180                 185                 190         
Ala Tyr His Ala Thr Ser Ala Pro Val Gln Ala Ile Asp Asp Phe Trp 
        195                 200                 205             
Gln Tyr Phe Asp Pro Ile Arg His Gly Met Ala Pro Asn Cys Ser Arg 
    210                 215                 220                 
Asp Val Ser Leu Val Ala Asn His Ile Asp Thr Val Gly Lys Asn Gly 
225                 230                 235                 240 
Ser Ala Ala Asp Gln Leu Ala Leu Lys Glu Leu Phe Gly Leu Glu Ala 
                245                 250                 255     
Leu Glu His Tyr Asp Asp Phe Ala Ala Ala Leu Pro Thr Gly Pro Tyr 
            260                 265                 270         
Leu Trp Gln Ser Asn Thr Phe Val Thr Gly Tyr Ser Asn Phe Phe Ala 
        275                 280                 285             
Phe Cys Asp Ala Val Glu Asn Val Glu Ala Gly Ala Ala Val Val Pro 
    290                 295                 300                 
Gly Pro Glu Gly Val Gly Leu Gln Lys Ala Leu Thr Gly Tyr Ala Asn 
305                 310                 315                 320 
Trp Phe Asn Ser Thr Ile Ile Pro Gly Tyr Cys Ala Ser Tyr Gly Tyr 
                325                 330                 335     
Trp Thr Asp Asn Arg Thr Val Ala Cys Phe Asp Thr His Asn Pro Ser 
            340                 345                 350         
Ser Ala Ile Phe Thr Asp Thr Ser Val Asp Asn Ala Val Asp Arg Gln 
        355                 360                 365             
Trp Gln Trp Phe Leu Cys Asn Glu Pro Phe Phe Trp Trp Gln Asp Gly 
    370                 375                 380                 
Ala Pro Glu Gly Val Pro Thr Ile Val Pro Arg Thr Ile Asn Ala Glu 
385                 390                 395                 400 
Tyr Trp Gln Arg Gln Cys Ser Leu Tyr Phe Pro Glu Val Asn Gly Tyr 
                405                 410                 415     
Thr Tyr Gly Ser Ala Lys Gly Lys Thr Ala Ala Thr Val Asn Thr Trp 
            420                 425                 430         
Thr Gly Gly Trp Ser Asp Ser Lys Asn Thr Ser Arg Leu Leu Trp Val 
        435                 440                 445             
Asn Gly Gln Tyr Asp Pro Trp Arg Asp Ser Gly Val Ser Ser Thr His 
    450                 455                 460                 
Arg Pro Gly Gly Pro Leu Thr Ser Thr Ala Asp Glu Pro Val Gln Val 
465                 470                 475                 480 
Ile Pro Gly Gly Phe His Cys Ser Asp Leu Tyr Leu Lys Asp Tyr Phe 
                485                 490                 495     
Ala Asn Ala Gly Val Lys Gln Val Val Asp Asn Ala Val Ala Gln Ile 
            500                 505                 510         
Lys Ser Trp Val Ala Glu Tyr Tyr Lys 
        515                 520     

<210> 12
<211> 1583
<212> DNA
<213> Artificial Sequence

<220> 
<221> source
<222> 1..1583
<223> /organism="Artificial Sequence"
      /note="Codon optimised bc2g101 for expression in A. niger"
      /mol_type="unassigned DNA"

<400> 12
atgcagttcc tgccccctct gtccattgtc accctccttg cctcctggcc ctccctctcc      60

cgtgccatcc accctcctcg ccccgtgcct cctcctgtca gccgccccgt cagcacccag     120

tcttccgtcg tcgagggcaa cgccaccttc gaacaattgc tcgaccacca cgactcttcc     180

aagggtacct tctcccagcg ttactggtgg agcaccgagt actggggtgg tcccggctct     240

cccgtcgtcc tcttcacccc cggtgaagcc tccgccgatg gatacgaggg ctacctcacc     300

aacaacaccc tgaccggcct ctacgctcag gagatccagg gtgccgtcat cctgattgag     360

caccgttact ggggagattc ttctccctac gaagaattga ctgctgagac tctccagtac     420

ctcaccctgg agcagtccat cctggatttg acccacttcg ctgagactgt gcagctggag     480

ttcgacacct ccaactcctc caacgccccc aaggctcctt gggtgcttgt cggtggcagc     540

tactctggtg ctcttgctgc ctggactgct gccgttgctc ccgagacttt ctgggcctac     600

cacgccacct ccgctcctgt gcaggccatt gatgacttct ggcagtactt cgaccccatc     660

cgtcacggaa tggctcccaa ctgctctcgt gatgtctccc tggttgccaa ccacattgac     720

actgtcggca agaacggctc tgctgccgac cagctggctc tcaaggagct gttcggtctt     780

gaggctcttg agcactacga cgacttcgct gctgcccttc ctactggtcc ctacctctgg     840

cagagcaaca ccttcgtcac tggctactcc aacttcttcg ctttctgcga tgccgttgag     900

aacgtcgagg ctggtgctgc tgttgttcct ggtcccgagg gtgtcggtct gcagaaggct     960

ctgaccggct acgccaactg gttcaactcg accatcatcc ccggatactg cgccagctac    1020

ggctactgga ccgacaaccg caccgttgcc tgcttcgaca cccacaaccc ctcctccgcc    1080

atcttcaccg acaccagcgt tgacaacgcc gtcgaccgcc agtggcagtg gttcctctgc    1140

aacgagccct tcttctggtg gcaggatggt gctcctgagg gtgttcccac catcgtcccc    1200

cgtaccatca acgcggaata ctggcagcgt cagtgctcgc tatacttccc tgaagtcaac    1260

ggctacacct acggcagcgc caagggcaag actgctgcta ctgtcaacac ctggactggt    1320

ggctggtccg acagcaagaa cacctcccgc ctcctctggg tgaacggcca gtacgacccc    1380

tggcgcgact ctggtgtctc ctccacccac cgccccggtg gtcccttgac ctccactgcc    1440

gatgagcccg tgcaggtcat ccctggtggt ttccactgct cggatctcta cctcaaggac    1500

tacttcgcca acgccggtgt caagcaggtt gtcgataacg ccgttgccca gatcaagagc    1560

tgggttgctg agtactacaa gta                                            1583


<210> 13
<211> 1568
<212> DNA
<213> Artificial sequence

<220> 
<221> source
<222> 1..1568
<223> /organism="Artificial Sequence"
      /note="BC2G102"
      /mol_type="unassigned DNA"

<400> 13
atggtcaagt ccatcctggc ctccgtcttc ttcgccgcca ctgctctggc tatccaccct      60

cctcgccccg tgcctcctcc tgtcagccgc cccgtcagca cccagtcttc cgtcgtcgag     120

ggcaacgcca ccttcgaaca attgctcgac caccacgact cttccaaggg taccttctcc     180

cagcgttact ggtggagcac cgagtactgg ggtggtcccg gctctcccgt cgtcctcttc     240

acccccggtg aagcctccgc cgatggatac gagggctacc tcaccaacaa caccctgacc     300

ggcctctacg ctcaggagat ccagggtgcc gtcatcctga ttgagcaccg ttactgggga     360

gattcttctc cctacgaaga attgactgct gagactctcc agtacctcac cctggagcag     420

tccatcctgg atttgaccca cttcgctgag actgtgcagc tggagttcga cacctccaac     480

tcctccaacg cccccaaggc tccttgggtg cttgtcggtg gcagctactc tggtgctctt     540

gctgcctgga ctgctgccgt tgctcccgag actttctggg cctaccacgc cacctccgct     600

cctgtgcagg ccattgatga cttctggcag tacttcgacc ccatccgtca cggaatggct     660

cccaactgct ctcgtgatgt ctccctggtt gccaaccaca ttgacactgt cggcaagaac     720

ggctctgctg ccgaccagct ggctctcaag gagctgttcg gtcttgaggc tcttgagcac     780

tacgacgact tcgctgctgc ccttcctact ggtccctacc tctggcagag caacaccttc     840

gtcactggct actccaactt cttcgctttc tgcgatgccg ttgagaacgt cgaggctggt     900

gctgctgttg ttcctggtcc cgagggtgtc ggtctgcaga aggctctgac cggctacgcc     960

aactggttca actcgaccat catccccgga tactgcgcca gctacggcta ctggaccgac    1020

aaccgcaccg ttgcctgctt cgacacccac aacccctcct ccgccatctt caccgacacc    1080

agcgttgaca acgccgtcga ccgccagtgg cagtggttcc tctgcaacga gcccttcttc    1140

tggtggcagg atggtgctcc tgagggtgtt cccaccatcg tcccccgtac catcaacgcg    1200

gaatactggc agcgtcagtg ctcgctatac ttccctgaag tcaacggcta cacctacggc    1260

agcgccaagg gcaagactgc tgctactgtc aacacctgga ctggtggctg gtccgacagc    1320

aagaacacct cccgcctcct ctgggtgaac ggccagtacg acccctggcg cgactctggt    1380

gtctcctcca cccaccgccc cggtggtccc ttgacctcca ctgccgatga gcccgtgcag    1440

gtcatccctg gtggtttcca ctgctcggat ctctacctca aggactactt cgccaacgcc    1500

ggtgtcaagc aggttgtcga taacgccgtt gcccagatca agagctgggt tgctgagtac    1560

tacaagta                                                             1568


<210> 14
<211> 1565
<212> DNA
<213> Artificial sequence

<220> 
<221> source
<222> 1..1565
<223> /organism="Artificial Sequence"
      /note="BC2G103"
      /mol_type="unassigned DNA"

<400> 14
atggtcaagt ccatcctggc ctccgtcttc ttcgccgcca ctgctctggc tgctcgcccc      60

cgcttggttc ccaagcccgt gtctcgtccc gccagcagca agtcggctgt cgtcgagggc     120

aacgccacct tcgaacaatt gctcgaccac cacgactctt ccaagggtac cttctcccag     180

cgttactggt ggagcaccga gtactggggt ggtcccggct ctcccgtcgt cctcttcacc     240

cccggtgaag cctccgccga tggatacgag ggctacctca ccaacaacac cctgaccggc     300

ctctacgctc aggagatcca gggtgccgtc atcctgattg agcaccgtta ctggggagat     360

tcttctccct acgaagaatt gactgctgag actctccagt acctcaccct ggagcagtcc     420

atcctggatt tgacccactt cgctgagact gtgcagctgg agttcgacac ctccaactcc     480

tccaacgccc ccaaggctcc ttgggtgctt gtcggtggca gctactctgg tgctcttgct     540

gcctggactg ctgccgttgc tcccgagact ttctgggcct accacgccac ctccgctcct     600

gtgcaggcca ttgatgactt ctggcagtac ttcgacccca tccgtcacgg aatggctccc     660

aactgctctc gtgatgtctc cctggttgcc aaccacattg acactgtcgg caagaacggc     720

tctgctgccg accagctggc tctcaaggag ctgttcggtc ttgaggctct tgagcactac     780

gacgacttcg ctgctgccct tcctactggt ccctacctct ggcagagcaa caccttcgtc     840

actggctact ccaacttctt cgctttctgc gatgccgttg agaacgtcga ggctggtgct     900

gctgttgttc ctggtcccga gggtgtcggt ctgcagaagg ctctgaccgg ctacgccaac     960

tggttcaact cgaccatcat ccccggatac tgcgccagct acggctactg gaccgacaac    1020

cgcaccgttg cctgcttcga cacccacaac ccctcctccg ccatcttcac cgacaccagc    1080

gttgacaacg ccgtcgaccg ccagtggcag tggttcctct gcaacgagcc cttcttctgg    1140

tggcaggatg gtgctcctga gggtgttccc accatcgtcc cccgtaccat caacgcggaa    1200

tactggcagc gtcagtgctc gctatacttc cctgaagtca acggctacac ctacggcagc    1260

gccaagggca agactgctgc tactgtcaac acctggactg gtggctggtc cgacagcaag    1320

aacacctccc gcctcctctg ggtgaacggc cagtacgacc cctggcgcga ctctggtgtc    1380

tcctccaccc accgccccgg tggtcccttg acctccactg ccgatgagcc cgtgcaggtc    1440

atccctggtg gtttccactg ctcggatctc tacctcaagg actacttcgc caacgccggt    1500

gtcaagcagg ttgtcgataa cgccgttgcc cagatcaaga gctgggttgc tgagtactac    1560

aagta                                                                1565


<210> 15
<211> 13
<212> DNA
<213> Artificial sequence

<220> 
<221> source
<222> 1..13
<223> /organism="Artificial Sequence"
      /note="translation initiation glaA promotor"
      /mol_type="unassigned DNA"

<400> 15
caccgtcaaa atg                                                       13


<210> 16
<211> 521
<212> PRT
<213> Artificial sequence

<220> 
<223> EDP Aspergillus niger 528-5, PmeA ss, EDP prosequence

<400> 16
Met Val Lys Ser Ile Leu Ala Ser Val Phe Phe Ala Ala Thr Ala Leu 
1               5                   10                  15      
Ala Ala Arg Pro Arg Leu Val Pro Lys Pro Val Ser Arg Pro Ala Ser 
            20                  25                  30          
Ser Lys Ser Ala Ala Thr Thr Gly Glu Ala Tyr Phe Glu Gln Leu Leu 
        35                  40                  45              
Asp His His Asn Pro Glu Lys Gly Thr Phe Ser Gln Arg Tyr Trp Trp 
    50                  55                  60                  
Ser Thr Glu Tyr Trp Gly Gly Pro Gly Ser Pro Val Val Leu Phe Thr 
65                  70                  75                  80  
Pro Gly Glu Val Ser Ala Asp Gly Tyr Glu Gly Tyr Leu Thr Asn Glu 
                85                  90                  95      
Thr Leu Thr Gly Val Tyr Ala Gln Glu Ile Gln Gly Ala Val Ile Leu 
            100                 105                 110         
Ile Glu His Arg Tyr Trp Gly Asp Ser Ser Pro Tyr Glu Val Leu Asn 
        115                 120                 125             
Ala Glu Thr Leu Gln Tyr Leu Thr Leu Asp Gln Ala Ile Leu Asp Met 
    130                 135                 140                 
Thr Tyr Phe Ala Glu Thr Val Lys Leu Gln Phe Asp Asn Ser Thr Arg 
145                 150                 155                 160 
Ser Asn Ala Gln Asn Ala Pro Trp Val Met Val Gly Gly Ser Tyr Ser 
                165                 170                 175     
Gly Ala Leu Thr Ala Trp Thr Glu Ser Val Ala Pro Gly Thr Phe Trp 
            180                 185                 190         
Ala Tyr His Ala Thr Ser Ala Pro Val Glu Ala Ile Tyr Asp Tyr Trp 
        195                 200                 205             
Gln Tyr Phe Tyr Pro Ile Gln Gln Gly Met Ala Gln Asn Cys Ser Lys 
    210                 215                 220                 
Asp Val Ser Leu Val Ala Glu Tyr Val Asp Lys Ile Gly Lys Asn Gly 
225                 230                 235                 240 
Thr Ala Lys Glu Gln Gln Ala Leu Lys Glu Leu Phe Gly Leu Gly Ala 
                245                 250                 255     
Val Glu His Phe Asp Asp Phe Ala Ala Val Leu Pro Asn Gly Pro Tyr 
            260                 265                 270         
Leu Trp Gln Asp Asn Asp Phe Ala Thr Gly Tyr Ser Ser Phe Phe Gln 
        275                 280                 285             
Phe Cys Asp Ala Val Glu Gly Val Glu Ala Gly Ala Ala Val Thr Pro 
    290                 295                 300                 
Gly Pro Glu Gly Val Gly Leu Glu Lys Ala Leu Ala Asn Tyr Ala Asn 
305                 310                 315                 320 
Trp Phe Asn Ser Thr Ile Leu Pro Asp Tyr Cys Ala Ser Tyr Gly Tyr 
                325                 330                 335     
Trp Thr Asp Glu Trp Ser Val Ala Cys Phe Asp Ser Tyr Asn Ala Ser 
            340                 345                 350         
Ser Pro Ile Tyr Thr Asp Thr Ser Val Gly Asn Ala Val Asp Arg Gln 
        355                 360                 365             
Trp Glu Trp Phe Leu Cys Asn Glu Pro Phe Phe Tyr Trp Gln Asp Gly 
    370                 375                 380                 
Ala Pro Glu Gly Thr Ser Thr Ile Val Pro Arg Leu Val Ser Ala Ser 
385                 390                 395                 400 
Tyr Trp Gln Arg Gln Cys Pro Leu Tyr Phe Pro Glu Thr Asn Gly Tyr 
                405                 410                 415     
Thr Tyr Gly Ser Ala Lys Gly Lys Asn Ala Ala Thr Val Asn Ser Trp 
            420                 425                 430         
Thr Gly Gly Trp Asp Met Thr Arg Asn Thr Thr Arg Leu Ile Trp Thr 
        435                 440                 445             
Asn Gly Gln Tyr Asp Pro Trp Arg Asp Ser Gly Val Ser Ser Thr Phe 
    450                 455                 460                 
Arg Pro Gly Gly Pro Leu Ala Ser Thr Ala Asn Glu Pro Val Gln Ile 
465                 470                 475                 480 
Ile Pro Gly Gly Phe His Cys Ser Asp Leu Tyr Met Ala Asp Tyr Tyr 
                485                 490                 495     
Ala Asn Glu Gly Val Lys Lys Val Val Asp Asn Glu Val Lys Gln Ile 
            500                 505                 510         
Lys Glu Trp Val Glu Glu Tyr Tyr Ala 
        515                 520     

<210> 17
<211> 1566
<212> DNA
<213> Artificial sequence

<220> 
<221> source
<222> 1..1566
<223> /organism="Artificial Sequence"
      /note="EDP Aspergillus niger 528-5 PmeA ss, EDP prosequence"
      /mol_type="unassigned DNA"

<400> 17
atggtcaagt ccatcctggc ctccgtcttc ttcgctgcca ctgctcttgc tgcaaggcct      60

cgtctcgttc ccaagcccgt ttctcgtccc gccagctcca agtccgctgc tactactggt     120

gaggcctact ttgaacagct gttggaccac cacaaccctg agaagggtac tttctcgcaa     180

agatactggt ggagcaccga gtactggggt ggtcccggat cccccgttgt cctgttcact     240

cccggtgagg tcagcgctga tggctacgag ggttatctga ccaacgagac tctcaccggt     300

gtctacgccc aggagattca gggtgctgtc atcctgatcg aacaccgata ctggggtgac     360

tcgtctccct acgaggtgct gaacgccgag actctccagt acttgaccct cgaccaggct     420

atccttgata tgacctactt cgccgaaacc gtcaagctcc agtttgacaa ctccacccgc     480

tccaacgctc agaacgctcc ttgggttatg gtcggcggca gctacagcgg tgctctgact     540

gcttggaccg agtccgttgc tcccggcacc ttctgggctt accacgccac ctctgctcct     600

gttgaggcca tctacgacta ctggcaatac ttctacccca ttcagcaggg tatggctcag     660

aactgctcca aagatgtctc tcttgtagca gaatacgtcg acaagatcgg caagaacggc     720

actgccaagg agcaacaggc tctgaaggag cttttcggcc taggagcagt ggagcacttc     780

gacgacttcg ccgctgttct gcccaacggt ccttacctct ggcaagacaa cgactttgcc     840

accggttact cttctttctt ccagttctgt gatgccgtcg agggtgtcga ggctggtgct     900

gccgtcaccc ccggtcctga aggtgttggt ctggaaaagg cccttgctaa ctacgcgaac     960

tggttcaact ctaccatcct ccccgattac tgcgccagct acggctactg gactgacgag    1020

tggtccgtcg cctgcttcga ctcctacaac gcctcctctc ctatatacac cgacaccagc    1080

gttggtaacg ccgtcgaccg tcagtgggag tggttcctct gcaatgagcc cttcttttac    1140

tggcaggacg gtgcccccga gggtacttca acgatagtac cccgcttagt gtccgcctcc    1200

tactggcagc gtcaatgtcc gttgtacttc cccgagacta acggttacac ctacggctcc    1260

gccaagggaa agaacgccgc caccgtcaac agctggaccg gtggctggga catgacccgt    1320

aacaccaccc gtctgatctg gacgaacggc caatacgacc cctggcgtga ctccggtgtc    1380

tcttccacct tccgccccgg tggtcccctc gcttcgaccg ccaacgagcc cgtccagata    1440

atacccggtg gtttccattg ctccgacctc tacatggcag actactacgc caacgagggc    1500

gtcaagaagg ttgtcgacaa cgaagtcaaa caaatcaagg agtgggttga ggaatactac    1560

gcgtaa                                                               1566


