                         SEQUENCE LISTING

<110>  Tocagen Inc.
 
<120>  A RETROVIRAL VECTOR HAVING IMMUNE-STIMULATING ACTIVITY

<130>  00014-021WO1

<140>  Not yet assigned
<141>  2015-03-25

<150>  US 61/970,823
<151>  2014-03-26

<160>  33    

<170>  PatentIn version 3.5

<210>  1
<211>  11892
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector pAC3-yCD2

<400>  1
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cggcgccagt cctccgattg      600

actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt      660

ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc      720

tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca      780

ccgggaggta agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac      840

tgattttatg cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg      900

tggtggaact gacgagttcg gaacacccgg ccgcaaccct gggagacgtc ccagggactt      960

cgggggccgt ttttgtggcc cgacctgagt ccaaaaatcc cgatcgtttt ggactctttg     1020

gtgcaccccc cttagaggag ggatatgtgg ttctggtagg agacgagaac ctaaaacagt     1080

tcccgcctcc gtctgaattt ttgctttcgg tttgggaccg aagccgcgcc gcgcgtcttg     1140

tctgctgcag catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaga     1200

atatgggcca gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg     1260

agcggatcgc tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct     1320

ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc     1380

tcatcaccca ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg     1440

tcccctacat cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct     1500

ttgtacaccc taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac     1560

ctcctcgttc gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg     1620

ccaaacctaa acctcaagtt ctttctgaca gtggggggcc gctcatcgac ctacttacag     1680

aagacccccc gccttatagg gacccaagac cacccccttc cgacagggac ggaaatggtg     1740

gagaagcgac ccctgcggga gaggcaccgg acccctcccc aatggcatct cgcctacgtg     1800

ggagacggga gccccctgtg gccgactcca ctacctcgca ggcattcccc ctccgcgcag     1860

gaggaaacgg acagcttcaa tactggccgt tctcctcttc tgacctttac aactggaaaa     1920

ataataaccc ttctttttct gaagatccag gtaaactgac agctctgatc gagtctgttc     1980

tcatcaccca tcagcccacc tgggacgact gtcagcagct gttggggact ctgctgaccg     2040

gagaagaaaa acaacgggtg ctcttagagg ctagaaaggc ggtgcggggc gatgatgggc     2100

gccccactca actgcccaat gaagtcgatg ccgcttttcc cctcgagcgc ccagactggg     2160

attacaccac ccaggcaggt aggaaccacc tagtccacta tcgccagttg ctcctagcgg     2220

gtctccaaaa cgcgggcaga agccccacca atttggccaa ggtaaaagga ataacacaag     2280

ggcccaatga gtctccctcg gccttcctag agagacttaa ggaagcctat cgcaggtaca     2340

ctccttatga ccctgaggac ccagggcaag aaactaatgt gtctatgtct ttcatttggc     2400

agtctgcccc agacattggg agaaagttag agaggttaga agatttaaaa aacaagacgc     2460

ttggagattt ggttagagag gcagaaaaga tctttaataa acgagaaacc ccggaagaaa     2520

gagaggaacg tatcaggaga gaaacagagg aaaaagaaga acgccgtagg acagaggatg     2580

agcagaaaga gaaagaaaga gatcgtagga gacatagaga gatgagcaag ctattggcca     2640

ctgtcgttag tggacagaaa caggatagac agggaggaga acgaaggagg tcccaactcg     2700

atcgcgacca gtgtgcctac tgcaaagaaa aggggcactg ggctaaagat tgtcccaaga     2760

aaccacgagg acctcgggga ccaagacccc agacctccct cctgacccta gatgactagg     2820

gaggtcaggg tcaggagccc ccccctgaac ccaggataac cctcaaagtc ggggggcaac     2880

ccgtcacctt cctggtagat actggggccc aacactccgt gctgacccaa aatcctggac     2940

ccctaagtga taagtctgcc tgggtccaag gggctactgg aggaaagcgg tatcgctgga     3000

ccacggatcg caaagtacat ctagctaccg gtaaggtcac ccactctttc ctccatgtac     3060

cagactgtcc ctatcctctg ttaggaagag atttgctgac taaactaaaa gcccaaatcc     3120

actttgaggg atcaggagcc caggttatgg gaccaatggg gcagcccctg caagtgttga     3180

ccctaaatat agaagatgag catcggctac atgagacctc aaaagagcca gatgtttctc     3240

tagggtccac atggctgtct gattttcctc aggcctgggc ggaaaccggg ggcatgggac     3300

tggcagttcg ccaagctcct ctgatcatac ctctgaaagc aacctctacc cccgtgtcca     3360

taaaacaata ccccatgtca caagaagcca gactggggat caagccccac atacagagac     3420

tgttggacca gggaatactg gtaccctgcc agtccccctg gaacacgccc ctgctacccg     3480

ttaagaaacc agggactaat gattataggc ctgtccagga tctgagagaa gtcaacaagc     3540

gggtggaaga catccacccc accgtgccca acccttacaa cctcttgagc gggctcccac     3600

cgtcccacca gtggtacact gtgcttgatt taaaggatgc ctttttctgc ctgagactcc     3660

accccaccag tcagcctctc ttcgcctttg agtggagaga tccagagatg ggaatctcag     3720

gacaattgac ctggaccaga ctcccacagg gtttcaaaaa cagtcccacc ctgtttgatg     3780

aggcactgca cagagaccta gcagacttcc ggatccagca cccagacttg atcctgctac     3840

agtacgtgga tgacttactg ctggccgcca cttctgagct agactgccaa caaggtactc     3900

gggccctgtt acaaacccta gggaacctcg ggtatcgggc ctcggccaag aaagcccaaa     3960

tttgccagaa acaggtcaag tatctggggt atcttctaaa agagggtcag agatggctga     4020

ctgaggccag aaaagagact gtgatggggc agcctactcc gaagacccct cgacaactaa     4080

gggagttcct agggacggca ggcttctgtc gcctctggat ccctgggttt gcagaaatgg     4140

cagccccctt gtaccctctc accaaaacgg ggactctgtt taattggggc ccagaccaac     4200

aaaaggccta tcaagaaatc aagcaagctc ttctaactgc cccagccctg gggttgccag     4260

atttgactaa gccctttgaa ctctttgtcg acgagaagca gggctacgcc aaaggtgtcc     4320

taacgcaaaa actgggacct tggcgtcggc cggtggccta cctgtccaaa aagctagacc     4380

cagtagcagc tgggtggccc ccttgcctac ggatggtagc agccattgcc gtactgacaa     4440

aggatgcagg caagctaacc atgggacagc cactagtcat tctggccccc catgcagtag     4500

aggcactagt caaacaaccc cccgaccgct ggctttccaa cgcccggatg actcactatc     4560

aggccttgct tttggacacg gaccgggtcc agttcggacc ggtggtagcc ctgaacccgg     4620

ctacgctgct cccactgcct gaggaagggc tgcaacacaa ctgccttgat atcctggccg     4680

aagcccacgg aacccgaccc gacctaacgg accagccgct cccagacgcc gaccacacct     4740

ggtacacgga tggaagcagt ctcttacaag agggacagcg taaggcggga gctgcggtga     4800

ccaccgagac cgaggtaatc tgggctaaag ccctgccagc cgggacatcc gctcagcggg     4860

ctgaactgat agcactcacc caggccctaa agatggcaga aggtaagaag ctaaatgttt     4920

atactgatag ccgttatgct tttgctactg cccatatcca tggagaaata tacagaaggc     4980

gtgggttgct cacatcagaa ggcaaagaga tcaaaaataa agacgagatc ttggccctac     5040

taaaagccct ctttctgccc aaaagactta gcataatcca ttgtccagga catcaaaagg     5100

gacacagcgc cgaggctaga ggcaaccgga tggctgacca agcggcccga aaggcagcca     5160

tcacagagac tccagacacc tctaccctcc tcatagaaaa ttcatcaccc tacacctcag     5220

aacattttca ttacacagtg actgatataa aggacctaac caagttgggg gccatttatg     5280

ataaaacaaa gaagtattgg gtctaccaag gaaaacctgt gatgcctgac cagtttactt     5340

ttgaattatt agactttctt catcagctga ctcacctcag cttctcaaaa atgaaggctc     5400

tcctagagag aagccacagt ccctactaca tgctgaaccg ggatcgaaca ctcaaaaata     5460

tcactgagac ctgcaaagct tgtgcacaag tcaacgccag caagtctgcc gttaaacagg     5520

gaactagggt ccgcgggcat cggcccggca ctcattggga gatcgatttc accgagataa     5580

agcccggatt gtatggctat aaatatcttc tagtttttat agataccttt tctggctgga     5640

tagaagcctt cccaaccaag aaagaaaccg ccaaggtcgt aaccaagaag ctactagagg     5700

agatcttccc caggttcggc atgcctcagg tattgggaac tgacaatggg cctgccttcg     5760

tctccaaggt gagtcagaca gtggccgatc tgttggggat tgattggaaa ttacattgtg     5820

catacagacc ccaaagctca ggccaggtag aaagaatgaa tagaaccatc aaggagactt     5880

taactaaatt aacgcttgca actggctcta gagactgggt gctcctactc cccttagccc     5940

tgtaccgagc ccgcaacacg ccgggccccc atggcctcac cccatatgag atcttatatg     6000

gggcaccccc gccccttgta aacttccctg accctgacat gacaagagtt actaacagcc     6060

cctctctcca agctcactta caggctctct acttagtcca gcacgaagtc tggagacctc     6120

tggcggcagc ctaccaagaa caactggacc gaccggtggt acctcaccct taccgagtcg     6180

gcgacacagt gtgggtccgc cgacaccaga ctaagaacct agaacctcgc tggaaaggac     6240

cttacacagt cctgctgacc acccccaccg ccctcaaagt agacggcatc gcagcttgga     6300

tacacgccgc ccacgtgaag gctgccgacc ccgggggtgg accatcctct agactgacat     6360

ggcgcgttca acgctctcaa aaccccctca agataagatt aacccgtgga agcccttaat     6420

agtcatggga gtcctgttag gagtagggat ggcagagagc ccccatcagg tctttaatgt     6480

aacctggaga gtcaccaacc tgatgactgg gcgtaccgcc aatgccacct ccctcctggg     6540

aactgtacaa gatgccttcc caaaattata ttttgatcta tgtgatctgg tcggagagga     6600

gtgggaccct tcagaccagg aaccgtatgt cgggtatggc tgcaagtacc ccgcagggag     6660

acagcggacc cggacttttg acttttacgt gtgccctggg cataccgtaa agtcggggtg     6720

tgggggacca ggagagggct actgtggtaa atgggggtgt gaaaccaccg gacaggctta     6780

ctggaagccc acatcatcgt gggacctaat ctcccttaag cgcggtaaca ccccctggga     6840

cacgggatgc tctaaagttg cctgtggccc ctgctacgac ctctccaaag tatccaattc     6900

cttccaaggg gctactcgag ggggcagatg caaccctcta gtcctagaat tcactgatgc     6960

aggaaaaaag gctaactggg acgggcccaa atcgtgggga ctgagactgt accggacagg     7020

aacagatcct attaccatgt tctccctgac ccggcaggtc cttaatgtgg gaccccgagt     7080

ccccataggg cccaacccag tattacccga ccaaagactc ccttcctcac caatagagat     7140

tgtaccggct ccacagccac ctagccccct caataccagt tacccccctt ccactaccag     7200

tacaccctca acctccccta caagtccaag tgtcccacag ccacccccag gaactggaga     7260

tagactacta gctctagtca aaggagccta tcaggcgctt aacctcacca atcccgacaa     7320

gacccaagaa tgttggctgt gcttagtgtc gggacctcct tattacgaag gagtagcggt     7380

cgtgggcact tataccaatc attccaccgc tccggccaac tgtacggcca cttcccaaca     7440

taagcttacc ctatctgaag tgacaggaca gggcctatgc atgggggcag tacctaaaac     7500

tcaccaggcc ttatgtaaca ccacccaaag cgccggctca ggatcctact accttgcagc     7560

acccgccgga acaatgtggg cttgcagcac tggattgact ccctgcttgt ccaccacggt     7620

gctcaatcta accacagatt attgtgtatt agttgaactc tggcccagag taatttacca     7680

ctcccccgat tatatgtatg gtcagcttga acagcgtacc aaatataaaa gagagccagt     7740

atcattgacc ctggcccttc tactaggagg attaaccatg ggagggattg cagctggaat     7800

agggacgggg accactgcct taattaaaac ccagcagttt gagcagcttc atgccgctat     7860

ccagacagac ctcaacgaag tcgaaaagtc aattaccaac ctagaaaagt cactgacctc     7920

gttgtctgaa gtagtcctac agaaccgcag aggcctagat ttgctattcc taaaggaggg     7980

aggtctctgc gcagccctaa aagaagaatg ttgtttttat gcagaccaca cggggctagt     8040

gagagacagc atggccaaat taagagaaag gcttaatcag agacaaaaac tatttgagac     8100

aggccaagga tggttcgaag ggctgtttaa tagatccccc tggtttacca ccttaatctc     8160

caccatcatg ggacctctaa tagtactctt actgatctta ctctttggac cttgcattct     8220

caatcgattg gtccaatttg ttaaagacag gatctcagtg gtccaggctc tggttttgac     8280

tcagcaatat caccagctaa aacccataga gtacgagcca tgaacgcgtt actggccgaa     8340

gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt     8400

cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg     8460

gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc     8520

ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc     8580

ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa     8640

aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc     8700

tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg     8760

gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac     8820

gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga ttataaatgg     8880

tgaccggcgg catggcctcc aagtgggatc aaaagggcat ggatatcgct tacgaggagg     8940

ccctgctggg ctacaaggag ggcggcgtgc ctatcggcgg ctgtctgatc aacaacaagg     9000

acggcagtgt gctgggcagg ggccacaaca tgaggttcca gaagggctcc gccaccctgc     9060

acggcgagat ctccaccctg gagaactgtg gcaggctgga gggcaaggtg tacaaggaca     9120

ccaccctgta caccaccctg tccccttgtg acatgtgtac cggcgctatc atcatgtacg     9180

gcatccctag gtgtgtgatc ggcgagaacg tgaacttcaa gtccaagggc gagaagtacc     9240

tgcaaaccag gggccacgag gtggtggttg ttgacgatga gaggtgtaag aagctgatga     9300

agcagttcat cgacgagagg cctcaggact ggttcgagga tatcggcgag taagcggccg     9360

cagataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc     9420

tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa     9480

ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagctga atatgggcca     9540

aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggaaca     9600

gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca     9660

agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg     9720

tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag     9780

ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa     9840

cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca     9900

ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct     9960

ctgagtgatt gactacccgt cagcgggggt ctttcattac atgtgagcaa aaggccagca    10020

aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc    10080

tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata    10140

aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc    10200

gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc    10260

acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga    10320

accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc    10380

ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag    10440

gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag    10500

gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag    10560

ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca    10620

gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga    10680

cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat    10740

cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga    10800

gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg    10860

tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga    10920

gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc    10980

agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac    11040

tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc    11100

agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc    11160

gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc    11220

catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt    11280

ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc    11340

atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg    11400

tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag    11460

cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat    11520

cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc    11580

atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa    11640

aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta    11700

ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa    11760

aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga    11820

aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct    11880

tcaagaattc at                                                        11892


<210>  2
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MLV-U3-B Primer

<400>  2
agcccacaac ccctcactc                                                    19


<210>  3
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MLV-Psi Primer

<400>  3
tctcccgatc ccggacga                                                     18


<210>  4
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Probe

<400>  4
ccccaaatga aagacccccg ctgacg                                            26


<210>  5
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' Env primer

<400>  5
ctgatcttac tctttggacc ttg                                               23


<210>  6
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' UTR Primer

<400>  6
cccctttttc tggagactaa ataa                                              24


<210>  7
<211>  11489
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector pAC3-H1-IDO1miR30shRNA2

<400>  7
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cggcgccagt cctccgattg      600

actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt      660

ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc      720

tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca      780

ccgggaggta agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac      840

tgattttatg cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg      900

tggtggaact gacgagttcg gaacacccgg ccgcaaccct gggagacgtc ccagggactt      960

cgggggccgt ttttgtggcc cgacctgagt ccaaaaatcc cgatcgtttt ggactctttg     1020

gtgcaccccc cttagaggag ggatatgtgg ttctggtagg agacgagaac ctaaaacagt     1080

tcccgcctcc gtctgaattt ttgctttcgg tttgggaccg aagccgcgcc gcgcgtcttg     1140

tctgctgcag catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa     1200

atatgggcca gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg     1260

agcggatcgc tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct     1320

ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc     1380

tcatcaccca ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg     1440

tcccctacat cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct     1500

ttgtacaccc taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac     1560

ctcctcgttc gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg     1620

ccaaacctaa acctcaagtt ctttctgaca gtggggggcc gctcatcgac ctacttacag     1680

aagacccccc gccttatagg gacccaagac cacccccttc cgacagggac ggaaatggtg     1740

gagaagcgac ccctgcggga gaggcaccgg acccctcccc aatggcatct cgcctacgtg     1800

ggagacggga gccccctgtg gccgactcca ctacctcgca ggcattcccc ctccgcgcag     1860

gaggaaacgg acagcttcaa tactggccgt tctcctcttc tgacctttac aactggaaaa     1920

ataataaccc ttctttttct gaagatccag gtaaactgac agctctgatc gagtctgtcc     1980

tcatcaccca tcagcccacc tgggacgact gtcagcagct gttggggact ctgctgaccg     2040

gagaagaaaa acaacgggtg ctcttagagg ctagaaaggc ggtgcggggc gatgatgggc     2100

gccccactca actgcccaat gaagtcgatg ccgcttttcc cctcgagcgc ccagactggg     2160

attacaccac ccaggcaggt aggaaccacc tagtccacta tcgccagttg ctcctagcgg     2220

gtctccaaaa cgcgggcaga agccccacca atttggccaa ggtaaaagga ataacacaag     2280

ggcccaatga gtctccctcg gccttcctag agagacttaa ggaagcctat cgcaggtaca     2340

ctccttatga ccctgaggac ccagggcaag aaactaatgt gtctatgtct ttcatttggc     2400

agtctgcccc agacattggg agaaagttag agaggttaga agatttaaaa aacaagacgc     2460

ttggagattt ggttagagag gcagaaaaga tctttaataa acgagaaacc ccggaagaaa     2520

gagaggaacg tatcaggaga gaaacagagg aaaaagaaga acgccgtagg acagaggatg     2580

agcagaaaga gaaagaaaga gatcgtagga gacatagaga gatgagcaag ctattggcca     2640

ctgtcgttag tggacagaaa caggatagac agggaggaga acgaaggagg tcccaactcg     2700

atcgcgacca gtgtgcctac tgcaaagaaa aggggcactg ggctaaagat tgtcccaaga     2760

aaccacgagg acctcgggga ccaagacccc agacctccct cctgacccta gatgactagg     2820

gaggtcaggg tcaggagccc ccccctgaac ccaggataac cctcaaagtc ggggggcaac     2880

ccgtcacctt cctggtagat actggggccc aacactccgt gctgacccaa aatcctggac     2940

ccctaagtga taagtctgcc tgggtccaag gggctactgg aggaaagcgg tatcgctgga     3000

ccacggatcg caaagtacat ctagctaccg gtaaggtcac ccactctttc ctccatgtac     3060

cagactgtcc ctatcctctg ttaggaagag atttgctgac taaactaaaa gcccaaatcc     3120

actttgaggg atcaggagcc caggttatgg gaccaatggg gcagcccctg caagtgttga     3180

ccctaaatat agaagatgag tatcggctac atgagacctc aaaagagcca gatgtttctc     3240

tagggtccac atggctgtct gattttcctc aggcctgggc ggaaaccggg ggcatgggac     3300

tggcagttcg ccaagctcct ctgatcatac ctctgaaagc aacctctacc cccgtgtcca     3360

taaaacaata ccccatgtca caagaagcca gactggggat caagccccac atacagagac     3420

tgttggacca gggaatactg gtaccctgcc agtccccctg gaacacgccc ctgctacccg     3480

ttaagaaacc agggactaat gattataggc ctgtccagga tctgagagaa gtcaacaagc     3540

gggtggaaga catccacccc accgtgccca acccttacaa cctcttgagc gggctcccac     3600

cgtcccacca gtggtacact gtgcttgatt taaaggatgc ctttttctgc ctgagactcc     3660

accccaccag tcagcctctc ttcgcctttg agtggagaga tccagagatg ggaatctcag     3720

gacaattgac ctggaccaga ctcccacagg gtttcaaaaa cagtcccacc ctgtttgatg     3780

aggcactgca cagagaccta gcagacttcc ggatccagca cccagacttg atcctgctac     3840

agtacgtgga tgacttactg ctggccgcca cttctgagct agactgccaa caaggtactc     3900

gggccctgtt acaaacccta gggaacctcg ggtatcgggc ctcggccaag aaagcccaaa     3960

tttgccagaa acaggtcaag tatctggggt atcttctaaa agagggtcag agatggctga     4020

ctgaggccag aaaagagact gtgatggggc agcctactcc gaagacccct cgacaactaa     4080

gggagttcct agggacggca ggcttctgtc gcctctggat ccctgggttt gcagaaatgg     4140

cagccccctt gtaccctctc accaaaacgg ggactctgtt taattggggc ccagaccaac     4200

aaaaggccta tcaagaaatc aagcaagctc ttctaactgc cccagccctg gggttgccag     4260

atttgactaa gccctttgaa ctctttgtcg acgagaagca gggctacgcc aaaggtgtcc     4320

taacgcaaaa actgggacct tggcgtcggc cggtggccta cctgtccaaa aagctagacc     4380

cagtagcagc tgggtggccc ccttgcctac ggatggtagc agccattgcc gtactgacaa     4440

aggatgcagg caagctaacc atgggacagc cactagtcat tctggccccc catgcagtag     4500

aggcactagt caaacaaccc cccgaccgct ggctttccaa cgcccggatg actcactatc     4560

aggccttgct tttggacacg gaccgggtcc agttcggacc ggtggtagcc ctgaacccgg     4620

ctacgctgct cccactgcct gaggaagggc tgcaacacaa ctgccttgat atcctggccg     4680

aagcccacgg aacccgaccc gacctaacgg accagccgct cccagacgcc gaccacacct     4740

ggtacacgga tggaagcagt ctcttacaag agggacagcg taaggcggga gctgcggtga     4800

ccaccgagac cgaggtaatc tgggctaaag ccctgccagc cgggacatcc gctcagcggg     4860

ctgaactgat agcactcacc caggccctaa agatggcaga aggtaagaag ctaaatgttt     4920

atactgatag ccgttatgct tttgctactg cccatatcca tggagaaata tacagaaggc     4980

gtgggttgct cacatcagaa ggcaaagaga tcaaaaataa agacgagatc ttggccctac     5040

taaaagccct ctttctgccc aaaagactta gcataatcca ttgtccagga catcaaaagg     5100

gacacagcgc cgaggctaga ggcaaccgga tggctgacca agcggcccga aaggcagcca     5160

tcacagagac tccagacacc tctaccctcc tcatagaaaa ttcatcaccc tacacctcag     5220

aacattttca ttacacagtg actgatataa aggacctaac caagttgggg gccatttatg     5280

ataaaacaaa gaagtattgg gtctaccaag gaaaacctgt gatgcctgac cagtttactt     5340

ttgaattatt agactttctt catcagctga ctcacctcag cttctcaaaa atgaaggctc     5400

tcctagagag aagccacagt ccctactaca tgctgaaccg ggatcgaaca ctcaaaaata     5460

tcactgagac ctgcaaagct tgtgcacaag tcaacgccag caagtctgcc gttaaacagg     5520

gaactagggt ccgcgggcat cggcccggca ctcattggga gatcgatttc accgagataa     5580

agcccggatt gtatggctat aaatatcttc tagtttttat agataccttt tctggctgga     5640

tagaagcctt cccaaccaag aaagaaaccg ccaaggtcgt aaccaagaag ctactagagg     5700

agatcttccc caggttcggc atgcctcagg tattgggaac tgacaatggg cctgccttcg     5760

tctccaaggt gagtcagaca gtggccgatc tgttggggat tgattggaaa ttacattgtg     5820

catacagacc ccaaagctca ggccaggtag aaagaatgaa tagaaccatc aaggagactt     5880

taactaaatt aacgcttgca actggctcta gagactgggt gctcctactc cccttagccc     5940

tgtaccgagc ccgcaacacg ccgggccccc atggcctcac cccatatgag atcttatatg     6000

gggcaccccc gccccttgta aacttccctg accctgacat gacaagagtt actaacagcc     6060

cctctctcca agctcactta caggctctct acttagtcca gcacgaagtc tggagacctc     6120

tggcggcagc ctaccaagaa caactggacc gaccggtggt acctcaccct taccgagtcg     6180

gcgacacagt gtgggtccgc cgacaccaga ctaagaacct agaacctcgc tggaaaggac     6240

cttacacagt cctgctgacc acccccaccg ccctcaaagt agacggcatc gcagcttgga     6300

tacacgccgc ccacgtgaag gctgccgacc ccgggggtgg accatcctct agactgacat     6360

ggcgcgttca acgctctcaa aaccccctca agataagatt aacccgtgga agcccttaat     6420

agtcatggga gtcctgttag gagtagggat ggcagagagc ccccatcagg tctttaatgt     6480

aacctggaga gtcaccaacc tgatgactgg gcgtaccgcc aatgccacct ccctcctggg     6540

aactgtacaa gatgccttcc caaaattata ttttgatcta tgtgatctgg tcggagagga     6600

gtgggaccct tcagaccagg aaccgtatgt cgggtatggc tgcaagtacc ccgcagggag     6660

acagcggacc cggacttttg acttttacgt gtgccctggg cataccgtaa agtcggggtg     6720

tgggggacca ggagagggct actgtggtaa atgggggtgt gaaaccaccg gacaggctta     6780

ctggaagccc acatcatcgt gggacctaat ctcccttaag cgcggtaaca ccccctggga     6840

cacgggatgc tctaaagttg cctgtggccc ctgctacgac ctctccaaag tatccaattc     6900

cttccaaggg gctactcgag ggggcagatg caaccctcta gtcctagaat tcactgatgc     6960

aggaaaaaag gctaactggg acgggcccaa atcgtgggga ctgagactgt accggacagg     7020

aacagatcct attaccatgt tctccctgac ccggcaggtc cttaatgtgg gaccccgagt     7080

ccccataggg cccaacccag tattacccga ccaaagactc ccttcctcac caatagagat     7140

tgtaccggct ccacagccac ctagccccct caataccagt tacccccctt ccactaccag     7200

tacaccctca acctccccta caagtccaag tgtcccacag ccacccccag gaactggaga     7260

tagactacta gctctagtca aaggagccta tcaggcgctt aacctcacca atcccgacaa     7320

gacccaagaa tgttggctgt gcttagtgtc gggacctcct tattacgaag gagtagcggt     7380

cgtgggcact tataccaatc attccaccgc tccggccaac tgtacggcca cttcccaaca     7440

taagcttacc ctatctgaag tgacaggaca gggcctatgc atgggggcag tacctaaaac     7500

tcaccaggcc ttatgtaaca ccacccaaag cgccggctca ggatcctact accttgcagc     7560

acccgccgga acaatgtggg cttgcagcac tggattgact ccctgcttgt ccaccacggt     7620

gctcaatcta accacagatt attgtgtatt agttgaactc tggcccagag taatttacca     7680

ctcccccgat tatatgtatg gtcagcttga acagcgtacc aaatataaaa gagagccagt     7740

atcattgacc ctggcccttc tactaggagg attaaccatg ggagggattg cagctggaat     7800

agggacgggg accactgcct taattaaaac ccagcagttt gagcagcttc atgccgctat     7860

ccagacagac ctcaacgaag tcgaaaagtc aattaccaac ctagaaaagt cactgacctc     7920

gttgtctgaa gtagtcctac agaaccgcag aggcctagat ttgctattcc taaaggaggg     7980

aggtctctgc gcagccctaa aagaagaatg ttgtttttat gcagaccaca cggggctagt     8040

gagagacagc atggccaaat taagagaaag gcttaatcag agacaaaaac tatttgagac     8100

aggccaagga tggttcgaag ggctgtttaa tagatccccc tggtttacca ccttaatctc     8160

caccatcatg ggacctctaa tagtactctt actgatctta ctctttggac cttgcattct     8220

caatcgattg gtccaatttg ttaaagacag gatctcagtg gtccaggctc tggttttgac     8280

tcagcaatat caccagctaa aacccataga gtacgagcca tgaacgcgtg aacgctgacg     8340

tcatcaaccc gctccaagga atcgcgggcc cagtgtcact aggcgggaac acccagcgcg     8400

cgtgcgccct ggcaggaaga tggctgtgag ggacagggga gtggcgccct gcaatatttg     8460

catgtcgcta tgtgttctgg gaaatcacca taaacgtgaa atgtctttgg atttgggaat     8520

cttataagtt ctgtatgaga ccactctttc ccggatcctg ctcgcttcgg cagcacatat     8580

actagtcgac tagggataac agggtaattg tttgaatgag gcttcagtac tttacagaat     8640

cgttgcctgc acatcttgga aacacttgct gggattactt cttcaggtta acccaacaga     8700

aggctcgaga aggtatattg ctgttgacag tgagcgacac gatcatgtga acccaaatag     8760

tgaagccaca gatgtatttg ggttcacatg atcgtggtgc ctactgcctc ggaattcaag     8820

gggctacttt aggagcaatt atcttgttta ctaaaactga ataccttgct atctctttga     8880

tacattttta caaagctgaa ttaaaatggt ataaattaaa tcactttttt caattggaag     8940

actaatgcgg cggccgcaga taaaataaaa gattttattt agtctccaga aaaagggggg     9000

aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc     9060

atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa cagatggaac     9120

agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc     9180

aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg     9240

ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct     9300

agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt     9360

tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca     9420

ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg     9480

ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc     9540

cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt cattacatgt     9600

gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc     9660

ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa     9720

acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc     9780

ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg     9840

cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc     9900

tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc     9960

gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca    10020

ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact    10080

acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg    10140

gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt    10200

ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct    10260

tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga    10320

gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa    10380

tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac    10440

ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga    10500

taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc    10560

cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca    10620

gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta    10680

gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg    10740

tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc    10800

gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg    10860

ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt    10920

ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt    10980

cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata    11040

ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc    11100

gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac    11160

ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa    11220

ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct    11280

tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat    11340

ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc    11400

cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca    11460

cgaggccctt tcgtcttcaa gaattccat                                      11489


<210>  8
<211>  11526
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector pAC3-U6-IDO1miRshRNA2

<400>  8
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cggcgccagt cctccgattg      600

actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt      660

ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc      720

tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca      780

ccgggaggta agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac      840

tgattttatg cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg      900

tggtggaact gacgagttcg gaacacccgg ccgcaaccct gggagacgtc ccagggactt      960

cgggggccgt ttttgtggcc cgacctgagt ccaaaaatcc cgatcgtttt ggactctttg     1020

gtgcaccccc cttagaggag ggatatgtgg ttctggtagg agacgagaac ctaaaacagt     1080

tcccgcctcc gtctgaattt ttgctttcgg tttgggaccg aagccgcgcc gcgcgtcttg     1140

tctgctgcag catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa     1200

atatgggcca gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg     1260

agcggatcgc tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct     1320

ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc     1380

tcatcaccca ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg     1440

tcccctacat cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct     1500

ttgtacaccc taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac     1560

ctcctcgttc gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg     1620

ccaaacctaa acctcaagtt ctttctgaca gtggggggcc gctcatcgac ctacttacag     1680

aagacccccc gccttatagg gacccaagac cacccccttc cgacagggac ggaaatggtg     1740

gagaagcgac ccctgcggga gaggcaccgg acccctcccc aatggcatct cgcctacgtg     1800

ggagacggga gccccctgtg gccgactcca ctacctcgca ggcattcccc ctccgcgcag     1860

gaggaaacgg acagcttcaa tactggccgt tctcctcttc tgacctttac aactggaaaa     1920

ataataaccc ttctttttct gaagatccag gtaaactgac agctctgatc gagtctgtcc     1980

tcatcaccca tcagcccacc tgggacgact gtcagcagct gttggggact ctgctgaccg     2040

gagaagaaaa acaacgggtg ctcttagagg ctagaaaggc ggtgcggggc gatgatgggc     2100

gccccactca actgcccaat gaagtcgatg ccgcttttcc cctcgagcgc ccagactggg     2160

attacaccac ccaggcaggt aggaaccacc tagtccacta tcgccagttg ctcctagcgg     2220

gtctccaaaa cgcgggcaga agccccacca atttggccaa ggtaaaagga ataacacaag     2280

ggcccaatga gtctccctcg gccttcctag agagacttaa ggaagcctat cgcaggtaca     2340

ctccttatga ccctgaggac ccagggcaag aaactaatgt gtctatgtct ttcatttggc     2400

agtctgcccc agacattggg agaaagttag agaggttaga agatttaaaa aacaagacgc     2460

ttggagattt ggttagagag gcagaaaaga tctttaataa acgagaaacc ccggaagaaa     2520

gagaggaacg tatcaggaga gaaacagagg aaaaagaaga acgccgtagg acagaggatg     2580

agcagaaaga gaaagaaaga gatcgtagga gacatagaga gatgagcaag ctattggcca     2640

ctgtcgttag tggacagaaa caggatagac agggaggaga acgaaggagg tcccaactcg     2700

atcgcgacca gtgtgcctac tgcaaagaaa aggggcactg ggctaaagat tgtcccaaga     2760

aaccacgagg acctcgggga ccaagacccc agacctccct cctgacccta gatgactagg     2820

gaggtcaggg tcaggagccc ccccctgaac ccaggataac cctcaaagtc ggggggcaac     2880

ccgtcacctt cctggtagat actggggccc aacactccgt gctgacccaa aatcctggac     2940

ccctaagtga taagtctgcc tgggtccaag gggctactgg aggaaagcgg tatcgctgga     3000

ccacggatcg caaagtacat ctagctaccg gtaaggtcac ccactctttc ctccatgtac     3060

cagactgtcc ctatcctctg ttaggaagag atttgctgac taaactaaaa gcccaaatcc     3120

actttgaggg atcaggagcc caggttatgg gaccaatggg gcagcccctg caagtgttga     3180

ccctaaatat agaagatgag tatcggctac atgagacctc aaaagagcca gatgtttctc     3240

tagggtccac atggctgtct gattttcctc aggcctgggc ggaaaccggg ggcatgggac     3300

tggcagttcg ccaagctcct ctgatcatac ctctgaaagc aacctctacc cccgtgtcca     3360

taaaacaata ccccatgtca caagaagcca gactggggat caagccccac atacagagac     3420

tgttggacca gggaatactg gtaccctgcc agtccccctg gaacacgccc ctgctacccg     3480

ttaagaaacc agggactaat gattataggc ctgtccagga tctgagagaa gtcaacaagc     3540

gggtggaaga catccacccc accgtgccca acccttacaa cctcttgagc gggctcccac     3600

cgtcccacca gtggtacact gtgcttgatt taaaggatgc ctttttctgc ctgagactcc     3660

accccaccag tcagcctctc ttcgcctttg agtggagaga tccagagatg ggaatctcag     3720

gacaattgac ctggaccaga ctcccacagg gtttcaaaaa cagtcccacc ctgtttgatg     3780

aggcactgca cagagaccta gcagacttcc ggatccagca cccagacttg atcctgctac     3840

agtacgtgga tgacttactg ctggccgcca cttctgagct agactgccaa caaggtactc     3900

gggccctgtt acaaacccta gggaacctcg ggtatcgggc ctcggccaag aaagcccaaa     3960

tttgccagaa acaggtcaag tatctggggt atcttctaaa agagggtcag agatggctga     4020

ctgaggccag aaaagagact gtgatggggc agcctactcc gaagacccct cgacaactaa     4080

gggagttcct agggacggca ggcttctgtc gcctctggat ccctgggttt gcagaaatgg     4140

cagccccctt gtaccctctc accaaaacgg ggactctgtt taattggggc ccagaccaac     4200

aaaaggccta tcaagaaatc aagcaagctc ttctaactgc cccagccctg gggttgccag     4260

atttgactaa gccctttgaa ctctttgtcg acgagaagca gggctacgcc aaaggtgtcc     4320

taacgcaaaa actgggacct tggcgtcggc cggtggccta cctgtccaaa aagctagacc     4380

cagtagcagc tgggtggccc ccttgcctac ggatggtagc agccattgcc gtactgacaa     4440

aggatgcagg caagctaacc atgggacagc cactagtcat tctggccccc catgcagtag     4500

aggcactagt caaacaaccc cccgaccgct ggctttccaa cgcccggatg actcactatc     4560

aggccttgct tttggacacg gaccgggtcc agttcggacc ggtggtagcc ctgaacccgg     4620

ctacgctgct cccactgcct gaggaagggc tgcaacacaa ctgccttgat atcctggccg     4680

aagcccacgg aacccgaccc gacctaacgg accagccgct cccagacgcc gaccacacct     4740

ggtacacgga tggaagcagt ctcttacaag agggacagcg taaggcggga gctgcggtga     4800

ccaccgagac cgaggtaatc tgggctaaag ccctgccagc cgggacatcc gctcagcggg     4860

ctgaactgat agcactcacc caggccctaa agatggcaga aggtaagaag ctaaatgttt     4920

atactgatag ccgttatgct tttgctactg cccatatcca tggagaaata tacagaaggc     4980

gtgggttgct cacatcagaa ggcaaagaga tcaaaaataa agacgagatc ttggccctac     5040

taaaagccct ctttctgccc aaaagactta gcataatcca ttgtccagga catcaaaagg     5100

gacacagcgc cgaggctaga ggcaaccgga tggctgacca agcggcccga aaggcagcca     5160

tcacagagac tccagacacc tctaccctcc tcatagaaaa ttcatcaccc tacacctcag     5220

aacattttca ttacacagtg actgatataa aggacctaac caagttgggg gccatttatg     5280

ataaaacaaa gaagtattgg gtctaccaag gaaaacctgt gatgcctgac cagtttactt     5340

ttgaattatt agactttctt catcagctga ctcacctcag cttctcaaaa atgaaggctc     5400

tcctagagag aagccacagt ccctactaca tgctgaaccg ggatcgaaca ctcaaaaata     5460

tcactgagac ctgcaaagct tgtgcacaag tcaacgccag caagtctgcc gttaaacagg     5520

gaactagggt ccgcgggcat cggcccggca ctcattggga gatcgatttc accgagataa     5580

agcccggatt gtatggctat aaatatcttc tagtttttat agataccttt tctggctgga     5640

tagaagcctt cccaaccaag aaagaaaccg ccaaggtcgt aaccaagaag ctactagagg     5700

agatcttccc caggttcggc atgcctcagg tattgggaac tgacaatggg cctgccttcg     5760

tctccaaggt gagtcagaca gtggccgatc tgttggggat tgattggaaa ttacattgtg     5820

catacagacc ccaaagctca ggccaggtag aaagaatgaa tagaaccatc aaggagactt     5880

taactaaatt aacgcttgca actggctcta gagactgggt gctcctactc cccttagccc     5940

tgtaccgagc ccgcaacacg ccgggccccc atggcctcac cccatatgag atcttatatg     6000

gggcaccccc gccccttgta aacttccctg accctgacat gacaagagtt actaacagcc     6060

cctctctcca agctcactta caggctctct acttagtcca gcacgaagtc tggagacctc     6120

tggcggcagc ctaccaagaa caactggacc gaccggtggt acctcaccct taccgagtcg     6180

gcgacacagt gtgggtccgc cgacaccaga ctaagaacct agaacctcgc tggaaaggac     6240

cttacacagt cctgctgacc acccccaccg ccctcaaagt agacggcatc gcagcttgga     6300

tacacgccgc ccacgtgaag gctgccgacc ccgggggtgg accatcctct agactgacat     6360

ggcgcgttca acgctctcaa aaccccctca agataagatt aacccgtgga agcccttaat     6420

agtcatggga gtcctgttag gagtagggat ggcagagagc ccccatcagg tctttaatgt     6480

aacctggaga gtcaccaacc tgatgactgg gcgtaccgcc aatgccacct ccctcctggg     6540

aactgtacaa gatgccttcc caaaattata ttttgatcta tgtgatctgg tcggagagga     6600

gtgggaccct tcagaccagg aaccgtatgt cgggtatggc tgcaagtacc ccgcagggag     6660

acagcggacc cggacttttg acttttacgt gtgccctggg cataccgtaa agtcggggtg     6720

tgggggacca ggagagggct actgtggtaa atgggggtgt gaaaccaccg gacaggctta     6780

ctggaagccc acatcatcgt gggacctaat ctcccttaag cgcggtaaca ccccctggga     6840

cacgggatgc tctaaagttg cctgtggccc ctgctacgac ctctccaaag tatccaattc     6900

cttccaaggg gctactcgag ggggcagatg caaccctcta gtcctagaat tcactgatgc     6960

aggaaaaaag gctaactggg acgggcccaa atcgtgggga ctgagactgt accggacagg     7020

aacagatcct attaccatgt tctccctgac ccggcaggtc cttaatgtgg gaccccgagt     7080

ccccataggg cccaacccag tattacccga ccaaagactc ccttcctcac caatagagat     7140

tgtaccggct ccacagccac ctagccccct caataccagt tacccccctt ccactaccag     7200

tacaccctca acctccccta caagtccaag tgtcccacag ccacccccag gaactggaga     7260

tagactacta gctctagtca aaggagccta tcaggcgctt aacctcacca atcccgacaa     7320

gacccaagaa tgttggctgt gcttagtgtc gggacctcct tattacgaag gagtagcggt     7380

cgtgggcact tataccaatc attccaccgc tccggccaac tgtacggcca cttcccaaca     7440

taagcttacc ctatctgaag tgacaggaca gggcctatgc atgggggcag tacctaaaac     7500

tcaccaggcc ttatgtaaca ccacccaaag cgccggctca ggatcctact accttgcagc     7560

acccgccgga acaatgtggg cttgcagcac tggattgact ccctgcttgt ccaccacggt     7620

gctcaatcta accacagatt attgtgtatt agttgaactc tggcccagag taatttacca     7680

ctcccccgat tatatgtatg gtcagcttga acagcgtacc aaatataaaa gagagccagt     7740

atcattgacc ctggcccttc tactaggagg attaaccatg ggagggattg cagctggaat     7800

agggacgggg accactgcct taattaaaac ccagcagttt gagcagcttc atgccgctat     7860

ccagacagac ctcaacgaag tcgaaaagtc aattaccaac ctagaaaagt cactgacctc     7920

gttgtctgaa gtagtcctac agaaccgcag aggcctagat ttgctattcc taaaggaggg     7980

aggtctctgc gcagccctaa aagaagaatg ttgtttttat gcagaccaca cggggctagt     8040

gagagacagc atggccaaat taagagaaag gcttaatcag agacaaaaac tatttgagac     8100

aggccaagga tggttcgaag ggctgtttaa tagatccccc tggtttacca ccttaatctc     8160

caccatcatg ggacctctaa tagtactctt actgatctta ctctttggac cttgcattct     8220

caatcgattg gtccaatttg ttaaagacag gatctcagtg gtccaggctc tggttttgac     8280

tcagcaatat caccagctaa aacccataga gtacgagcca tgaacgcgta aggtcgggca     8340

ggaagagggc ctatttccca tgattccttc atatttgcat atacgataca aggctgttag     8400

agagataatt agaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg     8460

tagaaagtaa taatttcttg ggtagtttgc agtttttaaa attatgtttt aaaatggact     8520

atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga     8580

aaggacgaaa caccgtgctc gcttcggcag cacatatact agtcgactag ggataacagg     8640

gtaattgttt gaatgaggct tcagtacttt acagaatcgt tgcctgcaca tcttggaaac     8700

acttgctggg attacttctt caggttaacc caacagaagg ctcgagaagg tatattgctg     8760

ttgacagtga gcgacacgat catgtgaacc caaatagtga agccacagat gtatttgggt     8820

tcacatgatc gtggtgccta ctgcctcgga attcaagggg ctactttagg agcaattatc     8880

ttgtttacta aaactgaata ccttgctatc tctttgatac atttttacaa agctgaatta     8940

aaatggtata aattaaatca cttttttcaa ttggaagact aatgcggcgg ccgcagataa     9000

aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt     9060

ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa     9120

tagagaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg ccaaacagga     9180

tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat     9240

atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag     9300

atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag     9360

ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt     9420

ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca     9480

ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc     9540

ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg     9600

attgactacc cgtcagcggg ggtctttcat tacatgtgag caaaaggcca gcaaaaggcc     9660

aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag     9720

catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac     9780

caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc     9840

ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt     9900

aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc     9960

gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga    10020

cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta    10080

ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta    10140

tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga    10200

tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg    10260

cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag    10320

tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc    10380

tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact    10440

tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt    10500

cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta    10560

ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta    10620

tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc    10680

gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat    10740

agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt    10800

atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg    10860

tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca    10920

gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta    10980

agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg    11040

cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact    11100

ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg    11160

ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt    11220

actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga    11280

ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc    11340

atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa    11400

caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt    11460

attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa    11520

ttccat                                                               11526


<210>  9
<211>  11528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector pAC3-RSV-IDO1miR30shRNA2

<400>  9
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cggcgccagt cctccgattg      600

actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt      660

ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc      720

tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca      780

ccgggaggta agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac      840

tgattttatg cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg      900

tggtggaact gacgagttcg gaacacccgg ccgcaaccct gggagacgtc ccagggactt      960

cgggggccgt ttttgtggcc cgacctgagt ccaaaaatcc cgatcgtttt ggactctttg     1020

gtgcaccccc cttagaggag ggatatgtgg ttctggtagg agacgagaac ctaaaacagt     1080

tcccgcctcc gtctgaattt ttgctttcgg tttgggaccg aagccgcgcc gcgcgtcttg     1140

tctgctgcag catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa     1200

atatgggcca gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg     1260

agcggatcgc tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct     1320

ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc     1380

tcatcaccca ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg     1440

tcccctacat cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct     1500

ttgtacaccc taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac     1560

ctcctcgttc gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg     1620

ccaaacctaa acctcaagtt ctttctgaca gtggggggcc gctcatcgac ctacttacag     1680

aagacccccc gccttatagg gacccaagac cacccccttc cgacagggac ggaaatggtg     1740

gagaagcgac ccctgcggga gaggcaccgg acccctcccc aatggcatct cgcctacgtg     1800

ggagacggga gccccctgtg gccgactcca ctacctcgca ggcattcccc ctccgcgcag     1860

gaggaaacgg acagcttcaa tactggccgt tctcctcttc tgacctttac aactggaaaa     1920

ataataaccc ttctttttct gaagatccag gtaaactgac agctctgatc gagtctgtcc     1980

tcatcaccca tcagcccacc tgggacgact gtcagcagct gttggggact ctgctgaccg     2040

gagaagaaaa acaacgggtg ctcttagagg ctagaaaggc ggtgcggggc gatgatgggc     2100

gccccactca actgcccaat gaagtcgatg ccgcttttcc cctcgagcgc ccagactggg     2160

attacaccac ccaggcaggt aggaaccacc tagtccacta tcgccagttg ctcctagcgg     2220

gtctccaaaa cgcgggcaga agccccacca atttggccaa ggtaaaagga ataacacaag     2280

ggcccaatga gtctccctcg gccttcctag agagacttaa ggaagcctat cgcaggtaca     2340

ctccttatga ccctgaggac ccagggcaag aaactaatgt gtctatgtct ttcatttggc     2400

agtctgcccc agacattggg agaaagttag agaggttaga agatttaaaa aacaagacgc     2460

ttggagattt ggttagagag gcagaaaaga tctttaataa acgagaaacc ccggaagaaa     2520

gagaggaacg tatcaggaga gaaacagagg aaaaagaaga acgccgtagg acagaggatg     2580

agcagaaaga gaaagaaaga gatcgtagga gacatagaga gatgagcaag ctattggcca     2640

ctgtcgttag tggacagaaa caggatagac agggaggaga acgaaggagg tcccaactcg     2700

atcgcgacca gtgtgcctac tgcaaagaaa aggggcactg ggctaaagat tgtcccaaga     2760

aaccacgagg acctcgggga ccaagacccc agacctccct cctgacccta gatgactagg     2820

gaggtcaggg tcaggagccc ccccctgaac ccaggataac cctcaaagtc ggggggcaac     2880

ccgtcacctt cctggtagat actggggccc aacactccgt gctgacccaa aatcctggac     2940

ccctaagtga taagtctgcc tgggtccaag gggctactgg aggaaagcgg tatcgctgga     3000

ccacggatcg caaagtacat ctagctaccg gtaaggtcac ccactctttc ctccatgtac     3060

cagactgtcc ctatcctctg ttaggaagag atttgctgac taaactaaaa gcccaaatcc     3120

actttgaggg atcaggagcc caggttatgg gaccaatggg gcagcccctg caagtgttga     3180

ccctaaatat agaagatgag tatcggctac atgagacctc aaaagagcca gatgtttctc     3240

tagggtccac atggctgtct gattttcctc aggcctgggc ggaaaccggg ggcatgggac     3300

tggcagttcg ccaagctcct ctgatcatac ctctgaaagc aacctctacc cccgtgtcca     3360

taaaacaata ccccatgtca caagaagcca gactggggat caagccccac atacagagac     3420

tgttggacca gggaatactg gtaccctgcc agtccccctg gaacacgccc ctgctacccg     3480

ttaagaaacc agggactaat gattataggc ctgtccagga tctgagagaa gtcaacaagc     3540

gggtggaaga catccacccc accgtgccca acccttacaa cctcttgagc gggctcccac     3600

cgtcccacca gtggtacact gtgcttgatt taaaggatgc ctttttctgc ctgagactcc     3660

accccaccag tcagcctctc ttcgcctttg agtggagaga tccagagatg ggaatctcag     3720

gacaattgac ctggaccaga ctcccacagg gtttcaaaaa cagtcccacc ctgtttgatg     3780

aggcactgca cagagaccta gcagacttcc ggatccagca cccagacttg atcctgctac     3840

agtacgtgga tgacttactg ctggccgcca cttctgagct agactgccaa caaggtactc     3900

gggccctgtt acaaacccta gggaacctcg ggtatcgggc ctcggccaag aaagcccaaa     3960

tttgccagaa acaggtcaag tatctggggt atcttctaaa agagggtcag agatggctga     4020

ctgaggccag aaaagagact gtgatggggc agcctactcc gaagacccct cgacaactaa     4080

gggagttcct agggacggca ggcttctgtc gcctctggat ccctgggttt gcagaaatgg     4140

cagccccctt gtaccctctc accaaaacgg ggactctgtt taattggggc ccagaccaac     4200

aaaaggccta tcaagaaatc aagcaagctc ttctaactgc cccagccctg gggttgccag     4260

atttgactaa gccctttgaa ctctttgtcg acgagaagca gggctacgcc aaaggtgtcc     4320

taacgcaaaa actgggacct tggcgtcggc cggtggccta cctgtccaaa aagctagacc     4380

cagtagcagc tgggtggccc ccttgcctac ggatggtagc agccattgcc gtactgacaa     4440

aggatgcagg caagctaacc atgggacagc cactagtcat tctggccccc catgcagtag     4500

aggcactagt caaacaaccc cccgaccgct ggctttccaa cgcccggatg actcactatc     4560

aggccttgct tttggacacg gaccgggtcc agttcggacc ggtggtagcc ctgaacccgg     4620

ctacgctgct cccactgcct gaggaagggc tgcaacacaa ctgccttgat atcctggccg     4680

aagcccacgg aacccgaccc gacctaacgg accagccgct cccagacgcc gaccacacct     4740

ggtacacgga tggaagcagt ctcttacaag agggacagcg taaggcggga gctgcggtga     4800

ccaccgagac cgaggtaatc tgggctaaag ccctgccagc cgggacatcc gctcagcggg     4860

ctgaactgat agcactcacc caggccctaa agatggcaga aggtaagaag ctaaatgttt     4920

atactgatag ccgttatgct tttgctactg cccatatcca tggagaaata tacagaaggc     4980

gtgggttgct cacatcagaa ggcaaagaga tcaaaaataa agacgagatc ttggccctac     5040

taaaagccct ctttctgccc aaaagactta gcataatcca ttgtccagga catcaaaagg     5100

gacacagcgc cgaggctaga ggcaaccgga tggctgacca agcggcccga aaggcagcca     5160

tcacagagac tccagacacc tctaccctcc tcatagaaaa ttcatcaccc tacacctcag     5220

aacattttca ttacacagtg actgatataa aggacctaac caagttgggg gccatttatg     5280

ataaaacaaa gaagtattgg gtctaccaag gaaaacctgt gatgcctgac cagtttactt     5340

ttgaattatt agactttctt catcagctga ctcacctcag cttctcaaaa atgaaggctc     5400

tcctagagag aagccacagt ccctactaca tgctgaaccg ggatcgaaca ctcaaaaata     5460

tcactgagac ctgcaaagct tgtgcacaag tcaacgccag caagtctgcc gttaaacagg     5520

gaactagggt ccgcgggcat cggcccggca ctcattggga gatcgatttc accgagataa     5580

agcccggatt gtatggctat aaatatcttc tagtttttat agataccttt tctggctgga     5640

tagaagcctt cccaaccaag aaagaaaccg ccaaggtcgt aaccaagaag ctactagagg     5700

agatcttccc caggttcggc atgcctcagg tattgggaac tgacaatggg cctgccttcg     5760

tctccaaggt gagtcagaca gtggccgatc tgttggggat tgattggaaa ttacattgtg     5820

catacagacc ccaaagctca ggccaggtag aaagaatgaa tagaaccatc aaggagactt     5880

taactaaatt aacgcttgca actggctcta gagactgggt gctcctactc cccttagccc     5940

tgtaccgagc ccgcaacacg ccgggccccc atggcctcac cccatatgag atcttatatg     6000

gggcaccccc gccccttgta aacttccctg accctgacat gacaagagtt actaacagcc     6060

cctctctcca agctcactta caggctctct acttagtcca gcacgaagtc tggagacctc     6120

tggcggcagc ctaccaagaa caactggacc gaccggtggt acctcaccct taccgagtcg     6180

gcgacacagt gtgggtccgc cgacaccaga ctaagaacct agaacctcgc tggaaaggac     6240

cttacacagt cctgctgacc acccccaccg ccctcaaagt agacggcatc gcagcttgga     6300

tacacgccgc ccacgtgaag gctgccgacc ccgggggtgg accatcctct agactgacat     6360

ggcgcgttca acgctctcaa aaccccctca agataagatt aacccgtgga agcccttaat     6420

agtcatggga gtcctgttag gagtagggat ggcagagagc ccccatcagg tctttaatgt     6480

aacctggaga gtcaccaacc tgatgactgg gcgtaccgcc aatgccacct ccctcctggg     6540

aactgtacaa gatgccttcc caaaattata ttttgatcta tgtgatctgg tcggagagga     6600

gtgggaccct tcagaccagg aaccgtatgt cgggtatggc tgcaagtacc ccgcagggag     6660

acagcggacc cggacttttg acttttacgt gtgccctggg cataccgtaa agtcggggtg     6720

tgggggacca ggagagggct actgtggtaa atgggggtgt gaaaccaccg gacaggctta     6780

ctggaagccc acatcatcgt gggacctaat ctcccttaag cgcggtaaca ccccctggga     6840

cacgggatgc tctaaagttg cctgtggccc ctgctacgac ctctccaaag tatccaattc     6900

cttccaaggg gctactcgag ggggcagatg caaccctcta gtcctagaat tcactgatgc     6960

aggaaaaaag gctaactggg acgggcccaa atcgtgggga ctgagactgt accggacagg     7020

aacagatcct attaccatgt tctccctgac ccggcaggtc cttaatgtgg gaccccgagt     7080

ccccataggg cccaacccag tattacccga ccaaagactc ccttcctcac caatagagat     7140

tgtaccggct ccacagccac ctagccccct caataccagt tacccccctt ccactaccag     7200

tacaccctca acctccccta caagtccaag tgtcccacag ccacccccag gaactggaga     7260

tagactacta gctctagtca aaggagccta tcaggcgctt aacctcacca atcccgacaa     7320

gacccaagaa tgttggctgt gcttagtgtc gggacctcct tattacgaag gagtagcggt     7380

cgtgggcact tataccaatc attccaccgc tccggccaac tgtacggcca cttcccaaca     7440

taagcttacc ctatctgaag tgacaggaca gggcctatgc atgggggcag tacctaaaac     7500

tcaccaggcc ttatgtaaca ccacccaaag cgccggctca ggatcctact accttgcagc     7560

acccgccgga acaatgtggg cttgcagcac tggattgact ccctgcttgt ccaccacggt     7620

gctcaatcta accacagatt attgtgtatt agttgaactc tggcccagag taatttacca     7680

ctcccccgat tatatgtatg gtcagcttga acagcgtacc aaatataaaa gagagccagt     7740

atcattgacc ctggcccttc tactaggagg attaaccatg ggagggattg cagctggaat     7800

agggacgggg accactgcct taattaaaac ccagcagttt gagcagcttc atgccgctat     7860

ccagacagac ctcaacgaag tcgaaaagtc aattaccaac ctagaaaagt cactgacctc     7920

gttgtctgaa gtagtcctac agaaccgcag aggcctagat ttgctattcc taaaggaggg     7980

aggtctctgc gcagccctaa aagaagaatg ttgtttttat gcagaccaca cggggctagt     8040

gagagacagc atggccaaat taagagaaag gcttaatcag agacaaaaac tatttgagac     8100

aggccaagga tggttcgaag ggctgtttaa tagatccccc tggtttacca ccttaatctc     8160

caccatcatg ggacctctaa tagtactctt actgatctta ctctttggac cttgcattct     8220

caatcgattg gtccaatttg ttaaagacag gatctcagtg gtccaggctc tggttttgac     8280

tcagcaatat caccagctaa aacccataga gtacgagcca tgaacgcgta atgtagtctt     8340

atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag     8400

gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat     8460

taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattc cgcattgcag     8520

agatattgta tttaagtgcc tagctcgata caataaacgc catttgacca ttcaccacat     8580

tggtgtgcac cggatcctgc tcgcttcggc agcacatata ctagtcgact agggataaca     8640

gggtaattgt ttgaatgagg cttcagtact ttacagaatc gttgcctgca catcttggaa     8700

acacttgctg ggattacttc ttcaggttaa cccaacagaa ggctcgagaa ggtatattgc     8760

tgttgacagt gagcgacacg atcatgtgaa cccaaatagt gaagccacag atgtatttgg     8820

gttcacatga tcgtggtgcc tactgcctcg gaattcaagg ggctacttta ggagcaatta     8880

tcttgtttac taaaactgaa taccttgcta tctctttgat acatttttac aaagctgaat     8940

taaaatggta taaattaaat cacttttttc aattggaaga ctaatgcggc ggccgcagat     9000

aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag     9060

gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag     9120

aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag     9180

gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga     9240

atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac     9300

agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc     9360

agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc     9420

ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct     9480

cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa     9540

ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag     9600

tgattgacta cccgtcagcg ggggtctttc attacatgtg agcaaaaggc cagcaaaagg     9660

ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg     9720

agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat     9780

accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta     9840

ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct     9900

gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc     9960

ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa    10020

gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg    10080

taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag    10140

tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt    10200

gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta    10260

cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc    10320

agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca    10380

cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa    10440

cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat    10500

ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct    10560

taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt    10620

tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat    10680

ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta    10740

atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg    10800

gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt    10860

tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg    10920

cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg    10980

taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc    11040

ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa    11100

ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac    11160

cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt    11220

ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg    11280

gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa    11340

gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata    11400

aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca    11460

ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag    11520

aattccat                                                             11528


<210>  10
<211>  12008
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector pAC3-RSV-yCD2-IDO1miR30shRNA2

<400>  10
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cggcgccagt cctccgattg      600

actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt      660

ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc      720

tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca      780

ccgggaggta agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac      840

tgattttatg cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg      900

tggtggaact gacgagttcg gaacacccgg ccgcaaccct gggagacgtc ccagggactt      960

cgggggccgt ttttgtggcc cgacctgagt ccaaaaatcc cgatcgtttt ggactctttg     1020

gtgcaccccc cttagaggag ggatatgtgg ttctggtagg agacgagaac ctaaaacagt     1080

tcccgcctcc gtctgaattt ttgctttcgg tttgggaccg aagccgcgcc gcgcgtcttg     1140

tctgctgcag catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa     1200

atatgggcca gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg     1260

agcggatcgc tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct     1320

ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc     1380

tcatcaccca ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg     1440

tcccctacat cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct     1500

ttgtacaccc taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac     1560

ctcctcgttc gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg     1620

ccaaacctaa acctcaagtt ctttctgaca gtggggggcc gctcatcgac ctacttacag     1680

aagacccccc gccttatagg gacccaagac cacccccttc cgacagggac ggaaatggtg     1740

gagaagcgac ccctgcggga gaggcaccgg acccctcccc aatggcatct cgcctacgtg     1800

ggagacggga gccccctgtg gccgactcca ctacctcgca ggcattcccc ctccgcgcag     1860

gaggaaacgg acagcttcaa tactggccgt tctcctcttc tgacctttac aactggaaaa     1920

ataataaccc ttctttttct gaagatccag gtaaactgac agctctgatc gagtctgtcc     1980

tcatcaccca tcagcccacc tgggacgact gtcagcagct gttggggact ctgctgaccg     2040

gagaagaaaa acaacgggtg ctcttagagg ctagaaaggc ggtgcggggc gatgatgggc     2100

gccccactca actgcccaat gaagtcgatg ccgcttttcc cctcgagcgc ccagactggg     2160

attacaccac ccaggcaggt aggaaccacc tagtccacta tcgccagttg ctcctagcgg     2220

gtctccaaaa cgcgggcaga agccccacca atttggccaa ggtaaaagga ataacacaag     2280

ggcccaatga gtctccctcg gccttcctag agagacttaa ggaagcctat cgcaggtaca     2340

ctccttatga ccctgaggac ccagggcaag aaactaatgt gtctatgtct ttcatttggc     2400

agtctgcccc agacattggg agaaagttag agaggttaga agatttaaaa aacaagacgc     2460

ttggagattt ggttagagag gcagaaaaga tctttaataa acgagaaacc ccggaagaaa     2520

gagaggaacg tatcaggaga gaaacagagg aaaaagaaga acgccgtagg acagaggatg     2580

agcagaaaga gaaagaaaga gatcgtagga gacatagaga gatgagcaag ctattggcca     2640

ctgtcgttag tggacagaaa caggatagac agggaggaga acgaaggagg tcccaactcg     2700

atcgcgacca gtgtgcctac tgcaaagaaa aggggcactg ggctaaagat tgtcccaaga     2760

aaccacgagg acctcgggga ccaagacccc agacctccct cctgacccta gatgactagg     2820

gaggtcaggg tcaggagccc ccccctgaac ccaggataac cctcaaagtc ggggggcaac     2880

ccgtcacctt cctggtagat actggggccc aacactccgt gctgacccaa aatcctggac     2940

ccctaagtga taagtctgcc tgggtccaag gggctactgg aggaaagcgg tatcgctgga     3000

ccacggatcg caaagtacat ctagctaccg gtaaggtcac ccactctttc ctccatgtac     3060

cagactgtcc ctatcctctg ttaggaagag atttgctgac taaactaaaa gcccaaatcc     3120

actttgaggg atcaggagcc caggttatgg gaccaatggg gcagcccctg caagtgttga     3180

ccctaaatat agaagatgag tatcggctac atgagacctc aaaagagcca gatgtttctc     3240

tagggtccac atggctgtct gattttcctc aggcctgggc ggaaaccggg ggcatgggac     3300

tggcagttcg ccaagctcct ctgatcatac ctctgaaagc aacctctacc cccgtgtcca     3360

taaaacaata ccccatgtca caagaagcca gactggggat caagccccac atacagagac     3420

tgttggacca gggaatactg gtaccctgcc agtccccctg gaacacgccc ctgctacccg     3480

ttaagaaacc agggactaat gattataggc ctgtccagga tctgagagaa gtcaacaagc     3540

gggtggaaga catccacccc accgtgccca acccttacaa cctcttgagc gggctcccac     3600

cgtcccacca gtggtacact gtgcttgatt taaaggatgc ctttttctgc ctgagactcc     3660

accccaccag tcagcctctc ttcgcctttg agtggagaga tccagagatg ggaatctcag     3720

gacaattgac ctggaccaga ctcccacagg gtttcaaaaa cagtcccacc ctgtttgatg     3780

aggcactgca cagagaccta gcagacttcc ggatccagca cccagacttg atcctgctac     3840

agtacgtgga tgacttactg ctggccgcca cttctgagct agactgccaa caaggtactc     3900

gggccctgtt acaaacccta gggaacctcg ggtatcgggc ctcggccaag aaagcccaaa     3960

tttgccagaa acaggtcaag tatctggggt atcttctaaa agagggtcag agatggctga     4020

ctgaggccag aaaagagact gtgatggggc agcctactcc gaagacccct cgacaactaa     4080

gggagttcct agggacggca ggcttctgtc gcctctggat ccctgggttt gcagaaatgg     4140

cagccccctt gtaccctctc accaaaacgg ggactctgtt taattggggc ccagaccaac     4200

aaaaggccta tcaagaaatc aagcaagctc ttctaactgc cccagccctg gggttgccag     4260

atttgactaa gccctttgaa ctctttgtcg acgagaagca gggctacgcc aaaggtgtcc     4320

taacgcaaaa actgggacct tggcgtcggc cggtggccta cctgtccaaa aagctagacc     4380

cagtagcagc tgggtggccc ccttgcctac ggatggtagc agccattgcc gtactgacaa     4440

aggatgcagg caagctaacc atgggacagc cactagtcat tctggccccc catgcagtag     4500

aggcactagt caaacaaccc cccgaccgct ggctttccaa cgcccggatg actcactatc     4560

aggccttgct tttggacacg gaccgggtcc agttcggacc ggtggtagcc ctgaacccgg     4620

ctacgctgct cccactgcct gaggaagggc tgcaacacaa ctgccttgat atcctggccg     4680

aagcccacgg aacccgaccc gacctaacgg accagccgct cccagacgcc gaccacacct     4740

ggtacacgga tggaagcagt ctcttacaag agggacagcg taaggcggga gctgcggtga     4800

ccaccgagac cgaggtaatc tgggctaaag ccctgccagc cgggacatcc gctcagcggg     4860

ctgaactgat agcactcacc caggccctaa agatggcaga aggtaagaag ctaaatgttt     4920

atactgatag ccgttatgct tttgctactg cccatatcca tggagaaata tacagaaggc     4980

gtgggttgct cacatcagaa ggcaaagaga tcaaaaataa agacgagatc ttggccctac     5040

taaaagccct ctttctgccc aaaagactta gcataatcca ttgtccagga catcaaaagg     5100

gacacagcgc cgaggctaga ggcaaccgga tggctgacca agcggcccga aaggcagcca     5160

tcacagagac tccagacacc tctaccctcc tcatagaaaa ttcatcaccc tacacctcag     5220

aacattttca ttacacagtg actgatataa aggacctaac caagttgggg gccatttatg     5280

ataaaacaaa gaagtattgg gtctaccaag gaaaacctgt gatgcctgac cagtttactt     5340

ttgaattatt agactttctt catcagctga ctcacctcag cttctcaaaa atgaaggctc     5400

tcctagagag aagccacagt ccctactaca tgctgaaccg ggatcgaaca ctcaaaaata     5460

tcactgagac ctgcaaagct tgtgcacaag tcaacgccag caagtctgcc gttaaacagg     5520

gaactagggt ccgcgggcat cggcccggca ctcattggga gatcgatttc accgagataa     5580

agcccggatt gtatggctat aaatatcttc tagtttttat agataccttt tctggctgga     5640

tagaagcctt cccaaccaag aaagaaaccg ccaaggtcgt aaccaagaag ctactagagg     5700

agatcttccc caggttcggc atgcctcagg tattgggaac tgacaatggg cctgccttcg     5760

tctccaaggt gagtcagaca gtggccgatc tgttggggat tgattggaaa ttacattgtg     5820

catacagacc ccaaagctca ggccaggtag aaagaatgaa tagaaccatc aaggagactt     5880

taactaaatt aacgcttgca actggctcta gagactgggt gctcctactc cccttagccc     5940

tgtaccgagc ccgcaacacg ccgggccccc atggcctcac cccatatgag atcttatatg     6000

gggcaccccc gccccttgta aacttccctg accctgacat gacaagagtt actaacagcc     6060

cctctctcca agctcactta caggctctct acttagtcca gcacgaagtc tggagacctc     6120

tggcggcagc ctaccaagaa caactggacc gaccggtggt acctcaccct taccgagtcg     6180

gcgacacagt gtgggtccgc cgacaccaga ctaagaacct agaacctcgc tggaaaggac     6240

cttacacagt cctgctgacc acccccaccg ccctcaaagt agacggcatc gcagcttgga     6300

tacacgccgc ccacgtgaag gctgccgacc ccgggggtgg accatcctct agactgacat     6360

ggcgcgttca acgctctcaa aaccccctca agataagatt aacccgtgga agcccttaat     6420

agtcatggga gtcctgttag gagtagggat ggcagagagc ccccatcagg tctttaatgt     6480

aacctggaga gtcaccaacc tgatgactgg gcgtaccgcc aatgccacct ccctcctggg     6540

aactgtacaa gatgccttcc caaaattata ttttgatcta tgtgatctgg tcggagagga     6600

gtgggaccct tcagaccagg aaccgtatgt cgggtatggc tgcaagtacc ccgcagggag     6660

acagcggacc cggacttttg acttttacgt gtgccctggg cataccgtaa agtcggggtg     6720

tgggggacca ggagagggct actgtggtaa atgggggtgt gaaaccaccg gacaggctta     6780

ctggaagccc acatcatcgt gggacctaat ctcccttaag cgcggtaaca ccccctggga     6840

cacgggatgc tctaaagttg cctgtggccc ctgctacgac ctctccaaag tatccaattc     6900

cttccaaggg gctactcgag ggggcagatg caaccctcta gtcctagaat tcactgatgc     6960

aggaaaaaag gctaactggg acgggcccaa atcgtgggga ctgagactgt accggacagg     7020

aacagatcct attaccatgt tctccctgac ccggcaggtc cttaatgtgg gaccccgagt     7080

ccccataggg cccaacccag tattacccga ccaaagactc ccttcctcac caatagagat     7140

tgtaccggct ccacagccac ctagccccct caataccagt tacccccctt ccactaccag     7200

tacaccctca acctccccta caagtccaag tgtcccacag ccacccccag gaactggaga     7260

tagactacta gctctagtca aaggagccta tcaggcgctt aacctcacca atcccgacaa     7320

gacccaagaa tgttggctgt gcttagtgtc gggacctcct tattacgaag gagtagcggt     7380

cgtgggcact tataccaatc attccaccgc tccggccaac tgtacggcca cttcccaaca     7440

taagcttacc ctatctgaag tgacaggaca gggcctatgc atgggggcag tacctaaaac     7500

tcaccaggcc ttatgtaaca ccacccaaag cgccggctca ggatcctact accttgcagc     7560

acccgccgga acaatgtggg cttgcagcac tggattgact ccctgcttgt ccaccacggt     7620

gctcaatcta accacagatt attgtgtatt agttgaactc tggcccagag taatttacca     7680

ctcccccgat tatatgtatg gtcagcttga acagcgtacc aaatataaaa gagagccagt     7740

atcattgacc ctggcccttc tactaggagg attaaccatg ggagggattg cagctggaat     7800

agggacgggg accactgcct taattaaaac ccagcagttt gagcagcttc atgccgctat     7860

ccagacagac ctcaacgaag tcgaaaagtc aattaccaac ctagaaaagt cactgacctc     7920

gttgtctgaa gtagtcctac agaaccgcag aggcctagat ttgctattcc taaaggaggg     7980

aggtctctgc gcagccctaa aagaagaatg ttgtttttat gcagaccaca cggggctagt     8040

gagagacagc atggccaaat taagagaaag gcttaatcag agacaaaaac tatttgagac     8100

aggccaagga tggttcgaag ggctgtttaa tagatccccc tggtttacca ccttaatctc     8160

caccatcatg ggacctctaa tagtactctt actgatctta ctctttggac cttgcattct     8220

caatcgattg gtccaatttg ttaaagacag gatctcagtg gtccaggctc tggttttgac     8280

tcagcaatat caccagctaa aacccataga gtacgagcca tgaacgcgta atgtagtctt     8340

atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag     8400

gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat     8460

taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattc cgcattgcag     8520

agatattgta tttaagtgcc tagctcgata caataaacgc catttgacca ttcaccacat     8580

tggtgtgcac catggtgacc ggcggcatgg cctccaagtg ggatcaaaag ggcatggata     8640

tcgcttacga ggaggccctg ctgggctaca aggagggcgg cgtgcctatc ggcggctgtc     8700

tgatcaacaa caaggacggc agtgtgctgg gcaggggcca caacatgagg ttccagaagg     8760

gctccgccac cctgcacggc gagatctcca ccctggagaa ctgtggcagg ctggagggca     8820

aggtgtacaa ggacaccacc ctgtacacca ccctgtcccc ttgtgacatg tgtaccggcg     8880

ctatcatcat gtacggcatc cctaggtgtg tgatcggcga gaacgtgaac ttcaagtcca     8940

agggcgagaa gtacctgcaa accaggggcc acgaggtggt ggttgttgac gatgagaggt     9000

gtaagaagct gatgaagcag ttcatcgacg agaggcctca ggactggttc gaggatatcg     9060

gcgagtaatg aggatcctgc tcgcttcggc agcacatata ctagtcgact agggataaca     9120

gggtaattgt ttgaatgagg cttcagtact ttacagaatc gttgcctgca catcttggaa     9180

acacttgctg ggattacttc ttcaggttaa cccaacagaa ggctcgagaa ggtatattgc     9240

tgttgacagt gagcgacacg atcatgtgaa cccaaatagt gaagccacag atgtatttgg     9300

gttcacatga tcgtggtgcc tactgcctcg gaattcaagg ggctacttta ggagcaatta     9360

tcttgtttac taaaactgaa taccttgcta tctctttgat acatttttac aaagctgaat     9420

taaaatggta taaattaaat cacttttttc aattggaaga ctaatgcggc ggccgcagat     9480

aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag     9540

gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag     9600

aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag     9660

gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga     9720

atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac     9780

agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc     9840

agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc     9900

ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct     9960

cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa    10020

ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag    10080

tgattgacta cccgtcagcg ggggtctttc attacatgtg agcaaaaggc cagcaaaagg    10140

ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg    10200

agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat    10260

accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta    10320

ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct    10380

gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc    10440

ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa    10500

gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg    10560

taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag    10620

tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt    10680

gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta    10740

cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc    10800

agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca    10860

cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa    10920

cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat    10980

ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct    11040

taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt    11100

tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat    11160

ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta    11220

atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg    11280

gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt    11340

tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg    11400

cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg    11460

taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc    11520

ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa    11580

ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac    11640

cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt    11700

ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg    11760

gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa    11820

gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata    11880

aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca    11940

ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag    12000

aattccat                                                             12008


<210>  11
<211>  12273
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector pAC3-RSV-yCD2-U6-IDO1miR30shRNA2

<400>  11
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cggcgccagt cctccgattg      600

actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt      660

ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc      720

tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca      780

ccgggaggta agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac      840

tgattttatg cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg      900

tggtggaact gacgagttcg gaacacccgg ccgcaaccct gggagacgtc ccagggactt      960

cgggggccgt ttttgtggcc cgacctgagt ccaaaaatcc cgatcgtttt ggactctttg     1020

gtgcaccccc cttagaggag ggatatgtgg ttctggtagg agacgagaac ctaaaacagt     1080

tcccgcctcc gtctgaattt ttgctttcgg tttgggaccg aagccgcgcc gcgcgtcttg     1140

tctgctgcag catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa     1200

atatgggcca gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg     1260

agcggatcgc tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct     1320

ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc     1380

tcatcaccca ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg     1440

tcccctacat cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct     1500

ttgtacaccc taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac     1560

ctcctcgttc gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg     1620

ccaaacctaa acctcaagtt ctttctgaca gtggggggcc gctcatcgac ctacttacag     1680

aagacccccc gccttatagg gacccaagac cacccccttc cgacagggac ggaaatggtg     1740

gagaagcgac ccctgcggga gaggcaccgg acccctcccc aatggcatct cgcctacgtg     1800

ggagacggga gccccctgtg gccgactcca ctacctcgca ggcattcccc ctccgcgcag     1860

gaggaaacgg acagcttcaa tactggccgt tctcctcttc tgacctttac aactggaaaa     1920

ataataaccc ttctttttct gaagatccag gtaaactgac agctctgatc gagtctgtcc     1980

tcatcaccca tcagcccacc tgggacgact gtcagcagct gttggggact ctgctgaccg     2040

gagaagaaaa acaacgggtg ctcttagagg ctagaaaggc ggtgcggggc gatgatgggc     2100

gccccactca actgcccaat gaagtcgatg ccgcttttcc cctcgagcgc ccagactggg     2160

attacaccac ccaggcaggt aggaaccacc tagtccacta tcgccagttg ctcctagcgg     2220

gtctccaaaa cgcgggcaga agccccacca atttggccaa ggtaaaagga ataacacaag     2280

ggcccaatga gtctccctcg gccttcctag agagacttaa ggaagcctat cgcaggtaca     2340

ctccttatga ccctgaggac ccagggcaag aaactaatgt gtctatgtct ttcatttggc     2400

agtctgcccc agacattggg agaaagttag agaggttaga agatttaaaa aacaagacgc     2460

ttggagattt ggttagagag gcagaaaaga tctttaataa acgagaaacc ccggaagaaa     2520

gagaggaacg tatcaggaga gaaacagagg aaaaagaaga acgccgtagg acagaggatg     2580

agcagaaaga gaaagaaaga gatcgtagga gacatagaga gatgagcaag ctattggcca     2640

ctgtcgttag tggacagaaa caggatagac agggaggaga acgaaggagg tcccaactcg     2700

atcgcgacca gtgtgcctac tgcaaagaaa aggggcactg ggctaaagat tgtcccaaga     2760

aaccacgagg acctcgggga ccaagacccc agacctccct cctgacccta gatgactagg     2820

gaggtcaggg tcaggagccc ccccctgaac ccaggataac cctcaaagtc ggggggcaac     2880

ccgtcacctt cctggtagat actggggccc aacactccgt gctgacccaa aatcctggac     2940

ccctaagtga taagtctgcc tgggtccaag gggctactgg aggaaagcgg tatcgctgga     3000

ccacggatcg caaagtacat ctagctaccg gtaaggtcac ccactctttc ctccatgtac     3060

cagactgtcc ctatcctctg ttaggaagag atttgctgac taaactaaaa gcccaaatcc     3120

actttgaggg atcaggagcc caggttatgg gaccaatggg gcagcccctg caagtgttga     3180

ccctaaatat agaagatgag tatcggctac atgagacctc aaaagagcca gatgtttctc     3240

tagggtccac atggctgtct gattttcctc aggcctgggc ggaaaccggg ggcatgggac     3300

tggcagttcg ccaagctcct ctgatcatac ctctgaaagc aacctctacc cccgtgtcca     3360

taaaacaata ccccatgtca caagaagcca gactggggat caagccccac atacagagac     3420

tgttggacca gggaatactg gtaccctgcc agtccccctg gaacacgccc ctgctacccg     3480

ttaagaaacc agggactaat gattataggc ctgtccagga tctgagagaa gtcaacaagc     3540

gggtggaaga catccacccc accgtgccca acccttacaa cctcttgagc gggctcccac     3600

cgtcccacca gtggtacact gtgcttgatt taaaggatgc ctttttctgc ctgagactcc     3660

accccaccag tcagcctctc ttcgcctttg agtggagaga tccagagatg ggaatctcag     3720

gacaattgac ctggaccaga ctcccacagg gtttcaaaaa cagtcccacc ctgtttgatg     3780

aggcactgca cagagaccta gcagacttcc ggatccagca cccagacttg atcctgctac     3840

agtacgtgga tgacttactg ctggccgcca cttctgagct agactgccaa caaggtactc     3900

gggccctgtt acaaacccta gggaacctcg ggtatcgggc ctcggccaag aaagcccaaa     3960

tttgccagaa acaggtcaag tatctggggt atcttctaaa agagggtcag agatggctga     4020

ctgaggccag aaaagagact gtgatggggc agcctactcc gaagacccct cgacaactaa     4080

gggagttcct agggacggca ggcttctgtc gcctctggat ccctgggttt gcagaaatgg     4140

cagccccctt gtaccctctc accaaaacgg ggactctgtt taattggggc ccagaccaac     4200

aaaaggccta tcaagaaatc aagcaagctc ttctaactgc cccagccctg gggttgccag     4260

atttgactaa gccctttgaa ctctttgtcg acgagaagca gggctacgcc aaaggtgtcc     4320

taacgcaaaa actgggacct tggcgtcggc cggtggccta cctgtccaaa aagctagacc     4380

cagtagcagc tgggtggccc ccttgcctac ggatggtagc agccattgcc gtactgacaa     4440

aggatgcagg caagctaacc atgggacagc cactagtcat tctggccccc catgcagtag     4500

aggcactagt caaacaaccc cccgaccgct ggctttccaa cgcccggatg actcactatc     4560

aggccttgct tttggacacg gaccgggtcc agttcggacc ggtggtagcc ctgaacccgg     4620

ctacgctgct cccactgcct gaggaagggc tgcaacacaa ctgccttgat atcctggccg     4680

aagcccacgg aacccgaccc gacctaacgg accagccgct cccagacgcc gaccacacct     4740

ggtacacgga tggaagcagt ctcttacaag agggacagcg taaggcggga gctgcggtga     4800

ccaccgagac cgaggtaatc tgggctaaag ccctgccagc cgggacatcc gctcagcggg     4860

ctgaactgat agcactcacc caggccctaa agatggcaga aggtaagaag ctaaatgttt     4920

atactgatag ccgttatgct tttgctactg cccatatcca tggagaaata tacagaaggc     4980

gtgggttgct cacatcagaa ggcaaagaga tcaaaaataa agacgagatc ttggccctac     5040

taaaagccct ctttctgccc aaaagactta gcataatcca ttgtccagga catcaaaagg     5100

gacacagcgc cgaggctaga ggcaaccgga tggctgacca agcggcccga aaggcagcca     5160

tcacagagac tccagacacc tctaccctcc tcatagaaaa ttcatcaccc tacacctcag     5220

aacattttca ttacacagtg actgatataa aggacctaac caagttgggg gccatttatg     5280

ataaaacaaa gaagtattgg gtctaccaag gaaaacctgt gatgcctgac cagtttactt     5340

ttgaattatt agactttctt catcagctga ctcacctcag cttctcaaaa atgaaggctc     5400

tcctagagag aagccacagt ccctactaca tgctgaaccg ggatcgaaca ctcaaaaata     5460

tcactgagac ctgcaaagct tgtgcacaag tcaacgccag caagtctgcc gttaaacagg     5520

gaactagggt ccgcgggcat cggcccggca ctcattggga gatcgatttc accgagataa     5580

agcccggatt gtatggctat aaatatcttc tagtttttat agataccttt tctggctgga     5640

tagaagcctt cccaaccaag aaagaaaccg ccaaggtcgt aaccaagaag ctactagagg     5700

agatcttccc caggttcggc atgcctcagg tattgggaac tgacaatggg cctgccttcg     5760

tctccaaggt gagtcagaca gtggccgatc tgttggggat tgattggaaa ttacattgtg     5820

catacagacc ccaaagctca ggccaggtag aaagaatgaa tagaaccatc aaggagactt     5880

taactaaatt aacgcttgca actggctcta gagactgggt gctcctactc cccttagccc     5940

tgtaccgagc ccgcaacacg ccgggccccc atggcctcac cccatatgag atcttatatg     6000

gggcaccccc gccccttgta aacttccctg accctgacat gacaagagtt actaacagcc     6060

cctctctcca agctcactta caggctctct acttagtcca gcacgaagtc tggagacctc     6120

tggcggcagc ctaccaagaa caactggacc gaccggtggt acctcaccct taccgagtcg     6180

gcgacacagt gtgggtccgc cgacaccaga ctaagaacct agaacctcgc tggaaaggac     6240

cttacacagt cctgctgacc acccccaccg ccctcaaagt agacggcatc gcagcttgga     6300

tacacgccgc ccacgtgaag gctgccgacc ccgggggtgg accatcctct agactgacat     6360

ggcgcgttca acgctctcaa aaccccctca agataagatt aacccgtgga agcccttaat     6420

agtcatggga gtcctgttag gagtagggat ggcagagagc ccccatcagg tctttaatgt     6480

aacctggaga gtcaccaacc tgatgactgg gcgtaccgcc aatgccacct ccctcctggg     6540

aactgtacaa gatgccttcc caaaattata ttttgatcta tgtgatctgg tcggagagga     6600

gtgggaccct tcagaccagg aaccgtatgt cgggtatggc tgcaagtacc ccgcagggag     6660

acagcggacc cggacttttg acttttacgt gtgccctggg cataccgtaa agtcggggtg     6720

tgggggacca ggagagggct actgtggtaa atgggggtgt gaaaccaccg gacaggctta     6780

ctggaagccc acatcatcgt gggacctaat ctcccttaag cgcggtaaca ccccctggga     6840

cacgggatgc tctaaagttg cctgtggccc ctgctacgac ctctccaaag tatccaattc     6900

cttccaaggg gctactcgag ggggcagatg caaccctcta gtcctagaat tcactgatgc     6960

aggaaaaaag gctaactggg acgggcccaa atcgtgggga ctgagactgt accggacagg     7020

aacagatcct attaccatgt tctccctgac ccggcaggtc cttaatgtgg gaccccgagt     7080

ccccataggg cccaacccag tattacccga ccaaagactc ccttcctcac caatagagat     7140

tgtaccggct ccacagccac ctagccccct caataccagt tacccccctt ccactaccag     7200

tacaccctca acctccccta caagtccaag tgtcccacag ccacccccag gaactggaga     7260

tagactacta gctctagtca aaggagccta tcaggcgctt aacctcacca atcccgacaa     7320

gacccaagaa tgttggctgt gcttagtgtc gggacctcct tattacgaag gagtagcggt     7380

cgtgggcact tataccaatc attccaccgc tccggccaac tgtacggcca cttcccaaca     7440

taagcttacc ctatctgaag tgacaggaca gggcctatgc atgggggcag tacctaaaac     7500

tcaccaggcc ttatgtaaca ccacccaaag cgccggctca ggatcctact accttgcagc     7560

acccgccgga acaatgtggg cttgcagcac tggattgact ccctgcttgt ccaccacggt     7620

gctcaatcta accacagatt attgtgtatt agttgaactc tggcccagag taatttacca     7680

ctcccccgat tatatgtatg gtcagcttga acagcgtacc aaatataaaa gagagccagt     7740

atcattgacc ctggcccttc tactaggagg attaaccatg ggagggattg cagctggaat     7800

agggacgggg accactgcct taattaaaac ccagcagttt gagcagcttc atgccgctat     7860

ccagacagac ctcaacgaag tcgaaaagtc aattaccaac ctagaaaagt cactgacctc     7920

gttgtctgaa gtagtcctac agaaccgcag aggcctagat ttgctattcc taaaggaggg     7980

aggtctctgc gcagccctaa aagaagaatg ttgtttttat gcagaccaca cggggctagt     8040

gagagacagc atggccaaat taagagaaag gcttaatcag agacaaaaac tatttgagac     8100

aggccaagga tggttcgaag ggctgtttaa tagatccccc tggtttacca ccttaatctc     8160

caccatcatg ggacctctaa tagtactctt actgatctta ctctttggac cttgcattct     8220

caatcgattg gtccaatttg ttaaagacag gatctcagtg gtccaggctc tggttttgac     8280

tcagcaatat caccagctaa aacccataga gtacgagcca tgaacgcgta atgtagtctt     8340

atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag     8400

gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat     8460

taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattc cgcattgcag     8520

agatattgta tttaagtgcc tagctcgata caataaacgc catttgacca ttcaccacat     8580

tggtgtgcac catggtgacc ggcggcatgg cctccaagtg ggatcaaaag ggcatggata     8640

tcgcttacga ggaggccctg ctgggctaca aggagggcgg cgtgcctatc ggcggctgtc     8700

tgatcaacaa caaggacggc agtgtgctgg gcaggggcca caacatgagg ttccagaagg     8760

gctccgccac cctgcacggc gagatctcca ccctggagaa ctgtggcagg ctggagggca     8820

aggtgtacaa ggacaccacc ctgtacacca ccctgtcccc ttgtgacatg tgtaccggcg     8880

ctatcatcat gtacggcatc cctaggtgtg tgatcggcga gaacgtgaac ttcaagtcca     8940

agggcgagaa gtacctgcaa accaggggcc acgaggtggt ggttgttgac gatgagaggt     9000

gtaagaagct gatgaagcag ttcatcgacg agaggcctca ggactggttc gaggatatcg     9060

gcgagtaatg aaaggtcggg caggaagagg gcctatttcc catgattcct tcatatttgc     9120

atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta aacacaaaga     9180

tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttta     9240

aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc     9300

ttggctttat atatcttgtg gaaaggacga aacaccggat cctgctcgct tcggcagcac     9360

atatactagt cgactaggga taacagggta attgtttgaa tgaggcttca gtactttaca     9420

gaatcgttgc ctgcacatct tggaaacact tgctgggatt acttcttcag gttaacccaa     9480

cagaaggctc gagaaggtat attgctgttg acagtgagcg acacgatcat gtgaacccaa     9540

atagtgaagc cacagatgta tttgggttca catgatcgtg gtgcctactg cctcggaatt     9600

caaggggcta ctttaggagc aattatcttg tttactaaaa ctgaatacct tgctatctct     9660

ttgatacatt tttacaaagc tgaattaaaa tggtataaat taaatcactt ttttcaattg     9720

gaagactaat gcggcggccg cagataaaat aaaagatttt atttagtctc cagaaaaagg     9780

ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca     9840

aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg     9900

gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag     9960

ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt    10020

cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt    10080

ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct    10140

tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag    10200

ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg    10260

cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct    10320

gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcattac    10380

atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt    10440

ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg    10500

cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc    10560

tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc    10620

gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc    10680

aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac    10740

tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt    10800

aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct    10860

aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc    10920

ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt    10980

ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg    11040

atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc    11100

atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa    11160

tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag    11220

gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg    11280

tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga    11340

gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag    11400

cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa    11460

gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc    11520

atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca    11580

aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg    11640

atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat    11700

aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc    11760

aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg    11820

gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg    11880

gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt    11940

gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca    12000

ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata    12060

ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac    12120

atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa    12180

gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt    12240

atcacgaggc cctttcgtct tcaagaattc cat                                 12273


<210>  12
<211>  11489
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector pAC3-H1-PDL1miR30shRN4

<400>  12
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cggcgccagt cctccgattg      600

actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt      660

ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc      720

tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca      780

ccgggaggta agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac      840

tgattttatg cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg      900

tggtggaact gacgagttcg gaacacccgg ccgcaaccct gggagacgtc ccagggactt      960

cgggggccgt ttttgtggcc cgacctgagt ccaaaaatcc cgatcgtttt ggactctttg     1020

gtgcaccccc cttagaggag ggatatgtgg ttctggtagg agacgagaac ctaaaacagt     1080

tcccgcctcc gtctgaattt ttgctttcgg tttgggaccg aagccgcgcc gcgcgtcttg     1140

tctgctgcag catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa     1200

atatgggcca gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg     1260

agcggatcgc tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct     1320

ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc     1380

tcatcaccca ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg     1440

tcccctacat cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct     1500

ttgtacaccc taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac     1560

ctcctcgttc gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg     1620

ccaaacctaa acctcaagtt ctttctgaca gtggggggcc gctcatcgac ctacttacag     1680

aagacccccc gccttatagg gacccaagac cacccccttc cgacagggac ggaaatggtg     1740

gagaagcgac ccctgcggga gaggcaccgg acccctcccc aatggcatct cgcctacgtg     1800

ggagacggga gccccctgtg gccgactcca ctacctcgca ggcattcccc ctccgcgcag     1860

gaggaaacgg acagcttcaa tactggccgt tctcctcttc tgacctttac aactggaaaa     1920

ataataaccc ttctttttct gaagatccag gtaaactgac agctctgatc gagtctgtcc     1980

tcatcaccca tcagcccacc tgggacgact gtcagcagct gttggggact ctgctgaccg     2040

gagaagaaaa acaacgggtg ctcttagagg ctagaaaggc ggtgcggggc gatgatgggc     2100

gccccactca actgcccaat gaagtcgatg ccgcttttcc cctcgagcgc ccagactggg     2160

attacaccac ccaggcaggt aggaaccacc tagtccacta tcgccagttg ctcctagcgg     2220

gtctccaaaa cgcgggcaga agccccacca atttggccaa ggtaaaagga ataacacaag     2280

ggcccaatga gtctccctcg gccttcctag agagacttaa ggaagcctat cgcaggtaca     2340

ctccttatga ccctgaggac ccagggcaag aaactaatgt gtctatgtct ttcatttggc     2400

agtctgcccc agacattggg agaaagttag agaggttaga agatttaaaa aacaagacgc     2460

ttggagattt ggttagagag gcagaaaaga tctttaataa acgagaaacc ccggaagaaa     2520

gagaggaacg tatcaggaga gaaacagagg aaaaagaaga acgccgtagg acagaggatg     2580

agcagaaaga gaaagaaaga gatcgtagga gacatagaga gatgagcaag ctattggcca     2640

ctgtcgttag tggacagaaa caggatagac agggaggaga acgaaggagg tcccaactcg     2700

atcgcgacca gtgtgcctac tgcaaagaaa aggggcactg ggctaaagat tgtcccaaga     2760

aaccacgagg acctcgggga ccaagacccc agacctccct cctgacccta gatgactagg     2820

gaggtcaggg tcaggagccc ccccctgaac ccaggataac cctcaaagtc ggggggcaac     2880

ccgtcacctt cctggtagat actggggccc aacactccgt gctgacccaa aatcctggac     2940

ccctaagtga taagtctgcc tgggtccaag gggctactgg aggaaagcgg tatcgctgga     3000

ccacggatcg caaagtacat ctagctaccg gtaaggtcac ccactctttc ctccatgtac     3060

cagactgtcc ctatcctctg ttaggaagag atttgctgac taaactaaaa gcccaaatcc     3120

actttgaggg atcaggagcc caggttatgg gaccaatggg gcagcccctg caagtgttga     3180

ccctaaatat agaagatgag tatcggctac atgagacctc aaaagagcca gatgtttctc     3240

tagggtccac atggctgtct gattttcctc aggcctgggc ggaaaccggg ggcatgggac     3300

tggcagttcg ccaagctcct ctgatcatac ctctgaaagc aacctctacc cccgtgtcca     3360

taaaacaata ccccatgtca caagaagcca gactggggat caagccccac atacagagac     3420

tgttggacca gggaatactg gtaccctgcc agtccccctg gaacacgccc ctgctacccg     3480

ttaagaaacc agggactaat gattataggc ctgtccagga tctgagagaa gtcaacaagc     3540

gggtggaaga catccacccc accgtgccca acccttacaa cctcttgagc gggctcccac     3600

cgtcccacca gtggtacact gtgcttgatt taaaggatgc ctttttctgc ctgagactcc     3660

accccaccag tcagcctctc ttcgcctttg agtggagaga tccagagatg ggaatctcag     3720

gacaattgac ctggaccaga ctcccacagg gtttcaaaaa cagtcccacc ctgtttgatg     3780

aggcactgca cagagaccta gcagacttcc ggatccagca cccagacttg atcctgctac     3840

agtacgtgga tgacttactg ctggccgcca cttctgagct agactgccaa caaggtactc     3900

gggccctgtt acaaacccta gggaacctcg ggtatcgggc ctcggccaag aaagcccaaa     3960

tttgccagaa acaggtcaag tatctggggt atcttctaaa agagggtcag agatggctga     4020

ctgaggccag aaaagagact gtgatggggc agcctactcc gaagacccct cgacaactaa     4080

gggagttcct agggacggca ggcttctgtc gcctctggat ccctgggttt gcagaaatgg     4140

cagccccctt gtaccctctc accaaaacgg ggactctgtt taattggggc ccagaccaac     4200

aaaaggccta tcaagaaatc aagcaagctc ttctaactgc cccagccctg gggttgccag     4260

atttgactaa gccctttgaa ctctttgtcg acgagaagca gggctacgcc aaaggtgtcc     4320

taacgcaaaa actgggacct tggcgtcggc cggtggccta cctgtccaaa aagctagacc     4380

cagtagcagc tgggtggccc ccttgcctac ggatggtagc agccattgcc gtactgacaa     4440

aggatgcagg caagctaacc atgggacagc cactagtcat tctggccccc catgcagtag     4500

aggcactagt caaacaaccc cccgaccgct ggctttccaa cgcccggatg actcactatc     4560

aggccttgct tttggacacg gaccgggtcc agttcggacc ggtggtagcc ctgaacccgg     4620

ctacgctgct cccactgcct gaggaagggc tgcaacacaa ctgccttgat atcctggccg     4680

aagcccacgg aacccgaccc gacctaacgg accagccgct cccagacgcc gaccacacct     4740

ggtacacgga tggaagcagt ctcttacaag agggacagcg taaggcggga gctgcggtga     4800

ccaccgagac cgaggtaatc tgggctaaag ccctgccagc cgggacatcc gctcagcggg     4860

ctgaactgat agcactcacc caggccctaa agatggcaga aggtaagaag ctaaatgttt     4920

atactgatag ccgttatgct tttgctactg cccatatcca tggagaaata tacagaaggc     4980

gtgggttgct cacatcagaa ggcaaagaga tcaaaaataa agacgagatc ttggccctac     5040

taaaagccct ctttctgccc aaaagactta gcataatcca ttgtccagga catcaaaagg     5100

gacacagcgc cgaggctaga ggcaaccgga tggctgacca agcggcccga aaggcagcca     5160

tcacagagac tccagacacc tctaccctcc tcatagaaaa ttcatcaccc tacacctcag     5220

aacattttca ttacacagtg actgatataa aggacctaac caagttgggg gccatttatg     5280

ataaaacaaa gaagtattgg gtctaccaag gaaaacctgt gatgcctgac cagtttactt     5340

ttgaattatt agactttctt catcagctga ctcacctcag cttctcaaaa atgaaggctc     5400

tcctagagag aagccacagt ccctactaca tgctgaaccg ggatcgaaca ctcaaaaata     5460

tcactgagac ctgcaaagct tgtgcacaag tcaacgccag caagtctgcc gttaaacagg     5520

gaactagggt ccgcgggcat cggcccggca ctcattggga gatcgatttc accgagataa     5580

agcccggatt gtatggctat aaatatcttc tagtttttat agataccttt tctggctgga     5640

tagaagcctt cccaaccaag aaagaaaccg ccaaggtcgt aaccaagaag ctactagagg     5700

agatcttccc caggttcggc atgcctcagg tattgggaac tgacaatggg cctgccttcg     5760

tctccaaggt gagtcagaca gtggccgatc tgttggggat tgattggaaa ttacattgtg     5820

catacagacc ccaaagctca ggccaggtag aaagaatgaa tagaaccatc aaggagactt     5880

taactaaatt aacgcttgca actggctcta gagactgggt gctcctactc cccttagccc     5940

tgtaccgagc ccgcaacacg ccgggccccc atggcctcac cccatatgag atcttatatg     6000

gggcaccccc gccccttgta aacttccctg accctgacat gacaagagtt actaacagcc     6060

cctctctcca agctcactta caggctctct acttagtcca gcacgaagtc tggagacctc     6120

tggcggcagc ctaccaagaa caactggacc gaccggtggt acctcaccct taccgagtcg     6180

gcgacacagt gtgggtccgc cgacaccaga ctaagaacct agaacctcgc tggaaaggac     6240

cttacacagt cctgctgacc acccccaccg ccctcaaagt agacggcatc gcagcttgga     6300

tacacgccgc ccacgtgaag gctgccgacc ccgggggtgg accatcctct agactgacat     6360

ggcgcgttca acgctctcaa aaccccctca agataagatt aacccgtgga agcccttaat     6420

agtcatggga gtcctgttag gagtagggat ggcagagagc ccccatcagg tctttaatgt     6480

aacctggaga gtcaccaacc tgatgactgg gcgtaccgcc aatgccacct ccctcctggg     6540

aactgtacaa gatgccttcc caaaattata ttttgatcta tgtgatctgg tcggagagga     6600

gtgggaccct tcagaccagg aaccgtatgt cgggtatggc tgcaagtacc ccgcagggag     6660

acagcggacc cggacttttg acttttacgt gtgccctggg cataccgtaa agtcggggtg     6720

tgggggacca ggagagggct actgtggtaa atgggggtgt gaaaccaccg gacaggctta     6780

ctggaagccc acatcatcgt gggacctaat ctcccttaag cgcggtaaca ccccctggga     6840

cacgggatgc tctaaagttg cctgtggccc ctgctacgac ctctccaaag tatccaattc     6900

cttccaaggg gctactcgag ggggcagatg caaccctcta gtcctagaat tcactgatgc     6960

aggaaaaaag gctaactggg acgggcccaa atcgtgggga ctgagactgt accggacagg     7020

aacagatcct attaccatgt tctccctgac ccggcaggtc cttaatgtgg gaccccgagt     7080

ccccataggg cccaacccag tattacccga ccaaagactc ccttcctcac caatagagat     7140

tgtaccggct ccacagccac ctagccccct caataccagt tacccccctt ccactaccag     7200

tacaccctca acctccccta caagtccaag tgtcccacag ccacccccag gaactggaga     7260

tagactacta gctctagtca aaggagccta tcaggcgctt aacctcacca atcccgacaa     7320

gacccaagaa tgttggctgt gcttagtgtc gggacctcct tattacgaag gagtagcggt     7380

cgtgggcact tataccaatc attccaccgc tccggccaac tgtacggcca cttcccaaca     7440

taagcttacc ctatctgaag tgacaggaca gggcctatgc atgggggcag tacctaaaac     7500

tcaccaggcc ttatgtaaca ccacccaaag cgccggctca ggatcctact accttgcagc     7560

acccgccgga acaatgtggg cttgcagcac tggattgact ccctgcttgt ccaccacggt     7620

gctcaatcta accacagatt attgtgtatt agttgaactc tggcccagag taatttacca     7680

ctcccccgat tatatgtatg gtcagcttga acagcgtacc aaatataaaa gagagccagt     7740

atcattgacc ctggcccttc tactaggagg attaaccatg ggagggattg cagctggaat     7800

agggacgggg accactgcct taattaaaac ccagcagttt gagcagcttc atgccgctat     7860

ccagacagac ctcaacgaag tcgaaaagtc aattaccaac ctagaaaagt cactgacctc     7920

gttgtctgaa gtagtcctac agaaccgcag aggcctagat ttgctattcc taaaggaggg     7980

aggtctctgc gcagccctaa aagaagaatg ttgtttttat gcagaccaca cggggctagt     8040

gagagacagc atggccaaat taagagaaag gcttaatcag agacaaaaac tatttgagac     8100

aggccaagga tggttcgaag ggctgtttaa tagatccccc tggtttacca ccttaatctc     8160

caccatcatg ggacctctaa tagtactctt actgatctta ctctttggac cttgcattct     8220

caatcgattg gtccaatttg ttaaagacag gatctcagtg gtccaggctc tggttttgac     8280

tcagcaatat caccagctaa aacccataga gtacgagcca tgaacgcgtg aacgctgacg     8340

tcatcaaccc gctccaagga atcgcgggcc cagtgtcact aggcgggaac acccagcgcg     8400

cgtgcgccct ggcaggaaga tggctgtgag ggacagggga gtggcgccct gcaatatttg     8460

catgtcgcta tgtgttctgg gaaatcacca taaacgtgaa atgtctttgg atttgggaat     8520

cttataagtt ctgtatgaga ccactctttc ccggatcctg ctcgcttcgg cagcacatat     8580

actagtcgac tagggataac agggtaattg tttgaatgag gcttcagtac tttacagaat     8640

cgttgcctgc acatcttgga aacacttgct gggattactt cttcaggtta acccaacaga     8700

aggctcgaga aggtatattg ctgttgacag tgagcgcggt agcaatatga caattgatag     8760

tgaagccaca gatgtatcaa ttgtcatatt gctaccatgc ctactgcctc ggaattcaag     8820

gggctacttt aggagcaatt atcttgttta ctaaaactga ataccttgct atctctttga     8880

tacattttta caaagctgaa ttaaaatggt ataaattaaa tcactttttt caattggaag     8940

actaatgcgg cggccgcaga taaaataaaa gattttattt agtctccaga aaaagggggg     9000

aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc     9060

atggaaaaat acataactga gaatagagaa gttcagatca aggtcaggaa cagatggaac     9120

agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc     9180

aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg     9240

ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct     9300

agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt     9360

tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca     9420

ataaaagagc ccacaacccc tcactcgggg cgccagtcct ccgattgact gagtcgcccg     9480

ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc     9540

cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt cattacatgt     9600

gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc     9660

ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa     9720

acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc     9780

ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg     9840

cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc     9900

tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc     9960

gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca    10020

ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact    10080

acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg    10140

gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt    10200

ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct    10260

tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga    10320

gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa    10380

tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac    10440

ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga    10500

taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc    10560

cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca    10620

gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta    10680

gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg    10740

tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc    10800

gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg    10860

ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt    10920

ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt    10980

cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata    11040

ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc    11100

gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac    11160

ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa    11220

ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct    11280

tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat    11340

ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc    11400

cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca    11460

cgaggccctt tcgtcttcaa gaattccat                                      11489


<210>  13
<211>  11526
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector pAC3-U6-PDL1-miR30shRNA4

<400>  13
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cggcgccagt cctccgattg      600

actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt      660

ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc      720

tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca      780

ccgggaggta agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac      840

tgattttatg cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg      900

tggtggaact gacgagttcg gaacacccgg ccgcaaccct gggagacgtc ccagggactt      960

cgggggccgt ttttgtggcc cgacctgagt ccaaaaatcc cgatcgtttt ggactctttg     1020

gtgcaccccc cttagaggag ggatatgtgg ttctggtagg agacgagaac ctaaaacagt     1080

tcccgcctcc gtctgaattt ttgctttcgg tttgggaccg aagccgcgcc gcgcgtcttg     1140

tctgctgcag catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa     1200

atatgggcca gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg     1260

agcggatcgc tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct     1320

ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc     1380

tcatcaccca ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg     1440

tcccctacat cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct     1500

ttgtacaccc taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac     1560

ctcctcgttc gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg     1620

ccaaacctaa acctcaagtt ctttctgaca gtggggggcc gctcatcgac ctacttacag     1680

aagacccccc gccttatagg gacccaagac cacccccttc cgacagggac ggaaatggtg     1740

gagaagcgac ccctgcggga gaggcaccgg acccctcccc aatggcatct cgcctacgtg     1800

ggagacggga gccccctgtg gccgactcca ctacctcgca ggcattcccc ctccgcgcag     1860

gaggaaacgg acagcttcaa tactggccgt tctcctcttc tgacctttac aactggaaaa     1920

ataataaccc ttctttttct gaagatccag gtaaactgac agctctgatc gagtctgtcc     1980

tcatcaccca tcagcccacc tgggacgact gtcagcagct gttggggact ctgctgaccg     2040

gagaagaaaa acaacgggtg ctcttagagg ctagaaaggc ggtgcggggc gatgatgggc     2100

gccccactca actgcccaat gaagtcgatg ccgcttttcc cctcgagcgc ccagactggg     2160

attacaccac ccaggcaggt aggaaccacc tagtccacta tcgccagttg ctcctagcgg     2220

gtctccaaaa cgcgggcaga agccccacca atttggccaa ggtaaaagga ataacacaag     2280

ggcccaatga gtctccctcg gccttcctag agagacttaa ggaagcctat cgcaggtaca     2340

ctccttatga ccctgaggac ccagggcaag aaactaatgt gtctatgtct ttcatttggc     2400

agtctgcccc agacattggg agaaagttag agaggttaga agatttaaaa aacaagacgc     2460

ttggagattt ggttagagag gcagaaaaga tctttaataa acgagaaacc ccggaagaaa     2520

gagaggaacg tatcaggaga gaaacagagg aaaaagaaga acgccgtagg acagaggatg     2580

agcagaaaga gaaagaaaga gatcgtagga gacatagaga gatgagcaag ctattggcca     2640

ctgtcgttag tggacagaaa caggatagac agggaggaga acgaaggagg tcccaactcg     2700

atcgcgacca gtgtgcctac tgcaaagaaa aggggcactg ggctaaagat tgtcccaaga     2760

aaccacgagg acctcgggga ccaagacccc agacctccct cctgacccta gatgactagg     2820

gaggtcaggg tcaggagccc ccccctgaac ccaggataac cctcaaagtc ggggggcaac     2880

ccgtcacctt cctggtagat actggggccc aacactccgt gctgacccaa aatcctggac     2940

ccctaagtga taagtctgcc tgggtccaag gggctactgg aggaaagcgg tatcgctgga     3000

ccacggatcg caaagtacat ctagctaccg gtaaggtcac ccactctttc ctccatgtac     3060

cagactgtcc ctatcctctg ttaggaagag atttgctgac taaactaaaa gcccaaatcc     3120

actttgaggg atcaggagcc caggttatgg gaccaatggg gcagcccctg caagtgttga     3180

ccctaaatat agaagatgag tatcggctac atgagacctc aaaagagcca gatgtttctc     3240

tagggtccac atggctgtct gattttcctc aggcctgggc ggaaaccggg ggcatgggac     3300

tggcagttcg ccaagctcct ctgatcatac ctctgaaagc aacctctacc cccgtgtcca     3360

taaaacaata ccccatgtca caagaagcca gactggggat caagccccac atacagagac     3420

tgttggacca gggaatactg gtaccctgcc agtccccctg gaacacgccc ctgctacccg     3480

ttaagaaacc agggactaat gattataggc ctgtccagga tctgagagaa gtcaacaagc     3540

gggtggaaga catccacccc accgtgccca acccttacaa cctcttgagc gggctcccac     3600

cgtcccacca gtggtacact gtgcttgatt taaaggatgc ctttttctgc ctgagactcc     3660

accccaccag tcagcctctc ttcgcctttg agtggagaga tccagagatg ggaatctcag     3720

gacaattgac ctggaccaga ctcccacagg gtttcaaaaa cagtcccacc ctgtttgatg     3780

aggcactgca cagagaccta gcagacttcc ggatccagca cccagacttg atcctgctac     3840

agtacgtgga tgacttactg ctggccgcca cttctgagct agactgccaa caaggtactc     3900

gggccctgtt acaaacccta gggaacctcg ggtatcgggc ctcggccaag aaagcccaaa     3960

tttgccagaa acaggtcaag tatctggggt atcttctaaa agagggtcag agatggctga     4020

ctgaggccag aaaagagact gtgatggggc agcctactcc gaagacccct cgacaactaa     4080

gggagttcct agggacggca ggcttctgtc gcctctggat ccctgggttt gcagaaatgg     4140

cagccccctt gtaccctctc accaaaacgg ggactctgtt taattggggc ccagaccaac     4200

aaaaggccta tcaagaaatc aagcaagctc ttctaactgc cccagccctg gggttgccag     4260

atttgactaa gccctttgaa ctctttgtcg acgagaagca gggctacgcc aaaggtgtcc     4320

taacgcaaaa actgggacct tggcgtcggc cggtggccta cctgtccaaa aagctagacc     4380

cagtagcagc tgggtggccc ccttgcctac ggatggtagc agccattgcc gtactgacaa     4440

aggatgcagg caagctaacc atgggacagc cactagtcat tctggccccc catgcagtag     4500

aggcactagt caaacaaccc cccgaccgct ggctttccaa cgcccggatg actcactatc     4560

aggccttgct tttggacacg gaccgggtcc agttcggacc ggtggtagcc ctgaacccgg     4620

ctacgctgct cccactgcct gaggaagggc tgcaacacaa ctgccttgat atcctggccg     4680

aagcccacgg aacccgaccc gacctaacgg accagccgct cccagacgcc gaccacacct     4740

ggtacacgga tggaagcagt ctcttacaag agggacagcg taaggcggga gctgcggtga     4800

ccaccgagac cgaggtaatc tgggctaaag ccctgccagc cgggacatcc gctcagcggg     4860

ctgaactgat agcactcacc caggccctaa agatggcaga aggtaagaag ctaaatgttt     4920

atactgatag ccgttatgct tttgctactg cccatatcca tggagaaata tacagaaggc     4980

gtgggttgct cacatcagaa ggcaaagaga tcaaaaataa agacgagatc ttggccctac     5040

taaaagccct ctttctgccc aaaagactta gcataatcca ttgtccagga catcaaaagg     5100

gacacagcgc cgaggctaga ggcaaccgga tggctgacca agcggcccga aaggcagcca     5160

tcacagagac tccagacacc tctaccctcc tcatagaaaa ttcatcaccc tacacctcag     5220

aacattttca ttacacagtg actgatataa aggacctaac caagttgggg gccatttatg     5280

ataaaacaaa gaagtattgg gtctaccaag gaaaacctgt gatgcctgac cagtttactt     5340

ttgaattatt agactttctt catcagctga ctcacctcag cttctcaaaa atgaaggctc     5400

tcctagagag aagccacagt ccctactaca tgctgaaccg ggatcgaaca ctcaaaaata     5460

tcactgagac ctgcaaagct tgtgcacaag tcaacgccag caagtctgcc gttaaacagg     5520

gaactagggt ccgcgggcat cggcccggca ctcattggga gatcgatttc accgagataa     5580

agcccggatt gtatggctat aaatatcttc tagtttttat agataccttt tctggctgga     5640

tagaagcctt cccaaccaag aaagaaaccg ccaaggtcgt aaccaagaag ctactagagg     5700

agatcttccc caggttcggc atgcctcagg tattgggaac tgacaatggg cctgccttcg     5760

tctccaaggt gagtcagaca gtggccgatc tgttggggat tgattggaaa ttacattgtg     5820

catacagacc ccaaagctca ggccaggtag aaagaatgaa tagaaccatc aaggagactt     5880

taactaaatt aacgcttgca actggctcta gagactgggt gctcctactc cccttagccc     5940

tgtaccgagc ccgcaacacg ccgggccccc atggcctcac cccatatgag atcttatatg     6000

gggcaccccc gccccttgta aacttccctg accctgacat gacaagagtt actaacagcc     6060

cctctctcca agctcactta caggctctct acttagtcca gcacgaagtc tggagacctc     6120

tggcggcagc ctaccaagaa caactggacc gaccggtggt acctcaccct taccgagtcg     6180

gcgacacagt gtgggtccgc cgacaccaga ctaagaacct agaacctcgc tggaaaggac     6240

cttacacagt cctgctgacc acccccaccg ccctcaaagt agacggcatc gcagcttgga     6300

tacacgccgc ccacgtgaag gctgccgacc ccgggggtgg accatcctct agactgacat     6360

ggcgcgttca acgctctcaa aaccccctca agataagatt aacccgtgga agcccttaat     6420

agtcatggga gtcctgttag gagtagggat ggcagagagc ccccatcagg tctttaatgt     6480

aacctggaga gtcaccaacc tgatgactgg gcgtaccgcc aatgccacct ccctcctggg     6540

aactgtacaa gatgccttcc caaaattata ttttgatcta tgtgatctgg tcggagagga     6600

gtgggaccct tcagaccagg aaccgtatgt cgggtatggc tgcaagtacc ccgcagggag     6660

acagcggacc cggacttttg acttttacgt gtgccctggg cataccgtaa agtcggggtg     6720

tgggggacca ggagagggct actgtggtaa atgggggtgt gaaaccaccg gacaggctta     6780

ctggaagccc acatcatcgt gggacctaat ctcccttaag cgcggtaaca ccccctggga     6840

cacgggatgc tctaaagttg cctgtggccc ctgctacgac ctctccaaag tatccaattc     6900

cttccaaggg gctactcgag ggggcagatg caaccctcta gtcctagaat tcactgatgc     6960

aggaaaaaag gctaactggg acgggcccaa atcgtgggga ctgagactgt accggacagg     7020

aacagatcct attaccatgt tctccctgac ccggcaggtc cttaatgtgg gaccccgagt     7080

ccccataggg cccaacccag tattacccga ccaaagactc ccttcctcac caatagagat     7140

tgtaccggct ccacagccac ctagccccct caataccagt tacccccctt ccactaccag     7200

tacaccctca acctccccta caagtccaag tgtcccacag ccacccccag gaactggaga     7260

tagactacta gctctagtca aaggagccta tcaggcgctt aacctcacca atcccgacaa     7320

gacccaagaa tgttggctgt gcttagtgtc gggacctcct tattacgaag gagtagcggt     7380

cgtgggcact tataccaatc attccaccgc tccggccaac tgtacggcca cttcccaaca     7440

taagcttacc ctatctgaag tgacaggaca gggcctatgc atgggggcag tacctaaaac     7500

tcaccaggcc ttatgtaaca ccacccaaag cgccggctca ggatcctact accttgcagc     7560

acccgccgga acaatgtggg cttgcagcac tggattgact ccctgcttgt ccaccacggt     7620

gctcaatcta accacagatt attgtgtatt agttgaactc tggcccagag taatttacca     7680

ctcccccgat tatatgtatg gtcagcttga acagcgtacc aaatataaaa gagagccagt     7740

atcattgacc ctggcccttc tactaggagg attaaccatg ggagggattg cagctggaat     7800

agggacgggg accactgcct taattaaaac ccagcagttt gagcagcttc atgccgctat     7860

ccagacagac ctcaacgaag tcgaaaagtc aattaccaac ctagaaaagt cactgacctc     7920

gttgtctgaa gtagtcctac agaaccgcag aggcctagat ttgctattcc taaaggaggg     7980

aggtctctgc gcagccctaa aagaagaatg ttgtttttat gcagaccaca cggggctagt     8040

gagagacagc atggccaaat taagagaaag gcttaatcag agacaaaaac tatttgagac     8100

aggccaagga tggttcgaag ggctgtttaa tagatccccc tggtttacca ccttaatctc     8160

caccatcatg ggacctctaa tagtactctt actgatctta ctctttggac cttgcattct     8220

caatcgattg gtccaatttg ttaaagacag gatctcagtg gtccaggctc tggttttgac     8280

tcagcaatat caccagctaa aacccataga gtacgagcca tgaacgcgta aggtcgggca     8340

ggaagagggc ctatttccca tgattccttc atatttgcat atacgataca aggctgttag     8400

agagataatt agaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg     8460

tagaaagtaa taatttcttg ggtagtttgc agtttttaaa attatgtttt aaaatggact     8520

atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga     8580

aaggacgaaa caccgtgctc gcttcggcag cacatatact agtcgactag ggataacagg     8640

gtaattgttt gaatgaggct tcagtacttt acagaatcgt tgcctgcaca tcttggaaac     8700

acttgctggg attacttctt caggttaacc caacagaagg ctcgagaagg tatattgctg     8760

ttgacagtga gcgcggtagc aatatgacaa ttgatagtga agccacagat gtatcaattg     8820

tcatattgct accatgccta ctgcctcgga attcaagggg ctactttagg agcaattatc     8880

ttgtttacta aaactgaata ccttgctatc tctttgatac atttttacaa agctgaatta     8940

aaatggtata aattaaatca cttttttcaa ttggaagact aatgcggcgg ccgcagataa     9000

aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt     9060

ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa     9120

tagagaagtt cagatcaagg tcaggaacag atggaacagc tgaatatggg ccaaacagga     9180

tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat     9240

atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag     9300

atggtcccca gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag     9360

ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt     9420

ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca     9480

ctcggggcgc cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc     9540

ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg     9600

attgactacc cgtcagcggg ggtctttcat tacatgtgag caaaaggcca gcaaaaggcc     9660

aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag     9720

catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac     9780

caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc     9840

ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt     9900

aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc     9960

gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga    10020

cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta    10080

ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta    10140

tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga    10200

tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg    10260

cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag    10320

tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc    10380

tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact    10440

tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt    10500

cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta    10560

ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta    10620

tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc    10680

gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat    10740

agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt    10800

atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg    10860

tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca    10920

gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta    10980

agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg    11040

cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact    11100

ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg    11160

ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt    11220

actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga    11280

ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc    11340

atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa    11400

caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt    11460

attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa    11520

ttccat                                                               11526


<210>  14
<211>  11528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector pAC3-RSV-PDL1miR30shRNA4

<400>  14
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cggcgccagt cctccgattg      600

actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt      660

ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc      720

tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca      780

ccgggaggta agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac      840

tgattttatg cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg      900

tggtggaact gacgagttcg gaacacccgg ccgcaaccct gggagacgtc ccagggactt      960

cgggggccgt ttttgtggcc cgacctgagt ccaaaaatcc cgatcgtttt ggactctttg     1020

gtgcaccccc cttagaggag ggatatgtgg ttctggtagg agacgagaac ctaaaacagt     1080

tcccgcctcc gtctgaattt ttgctttcgg tttgggaccg aagccgcgcc gcgcgtcttg     1140

tctgctgcag catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa     1200

atatgggcca gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg     1260

agcggatcgc tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct     1320

ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc     1380

tcatcaccca ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg     1440

tcccctacat cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct     1500

ttgtacaccc taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac     1560

ctcctcgttc gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg     1620

ccaaacctaa acctcaagtt ctttctgaca gtggggggcc gctcatcgac ctacttacag     1680

aagacccccc gccttatagg gacccaagac cacccccttc cgacagggac ggaaatggtg     1740

gagaagcgac ccctgcggga gaggcaccgg acccctcccc aatggcatct cgcctacgtg     1800

ggagacggga gccccctgtg gccgactcca ctacctcgca ggcattcccc ctccgcgcag     1860

gaggaaacgg acagcttcaa tactggccgt tctcctcttc tgacctttac aactggaaaa     1920

ataataaccc ttctttttct gaagatccag gtaaactgac agctctgatc gagtctgtcc     1980

tcatcaccca tcagcccacc tgggacgact gtcagcagct gttggggact ctgctgaccg     2040

gagaagaaaa acaacgggtg ctcttagagg ctagaaaggc ggtgcggggc gatgatgggc     2100

gccccactca actgcccaat gaagtcgatg ccgcttttcc cctcgagcgc ccagactggg     2160

attacaccac ccaggcaggt aggaaccacc tagtccacta tcgccagttg ctcctagcgg     2220

gtctccaaaa cgcgggcaga agccccacca atttggccaa ggtaaaagga ataacacaag     2280

ggcccaatga gtctccctcg gccttcctag agagacttaa ggaagcctat cgcaggtaca     2340

ctccttatga ccctgaggac ccagggcaag aaactaatgt gtctatgtct ttcatttggc     2400

agtctgcccc agacattggg agaaagttag agaggttaga agatttaaaa aacaagacgc     2460

ttggagattt ggttagagag gcagaaaaga tctttaataa acgagaaacc ccggaagaaa     2520

gagaggaacg tatcaggaga gaaacagagg aaaaagaaga acgccgtagg acagaggatg     2580

agcagaaaga gaaagaaaga gatcgtagga gacatagaga gatgagcaag ctattggcca     2640

ctgtcgttag tggacagaaa caggatagac agggaggaga acgaaggagg tcccaactcg     2700

atcgcgacca gtgtgcctac tgcaaagaaa aggggcactg ggctaaagat tgtcccaaga     2760

aaccacgagg acctcgggga ccaagacccc agacctccct cctgacccta gatgactagg     2820

gaggtcaggg tcaggagccc ccccctgaac ccaggataac cctcaaagtc ggggggcaac     2880

ccgtcacctt cctggtagat actggggccc aacactccgt gctgacccaa aatcctggac     2940

ccctaagtga taagtctgcc tgggtccaag gggctactgg aggaaagcgg tatcgctgga     3000

ccacggatcg caaagtacat ctagctaccg gtaaggtcac ccactctttc ctccatgtac     3060

cagactgtcc ctatcctctg ttaggaagag atttgctgac taaactaaaa gcccaaatcc     3120

actttgaggg atcaggagcc caggttatgg gaccaatggg gcagcccctg caagtgttga     3180

ccctaaatat agaagatgag tatcggctac atgagacctc aaaagagcca gatgtttctc     3240

tagggtccac atggctgtct gattttcctc aggcctgggc ggaaaccggg ggcatgggac     3300

tggcagttcg ccaagctcct ctgatcatac ctctgaaagc aacctctacc cccgtgtcca     3360

taaaacaata ccccatgtca caagaagcca gactggggat caagccccac atacagagac     3420

tgttggacca gggaatactg gtaccctgcc agtccccctg gaacacgccc ctgctacccg     3480

ttaagaaacc agggactaat gattataggc ctgtccagga tctgagagaa gtcaacaagc     3540

gggtggaaga catccacccc accgtgccca acccttacaa cctcttgagc gggctcccac     3600

cgtcccacca gtggtacact gtgcttgatt taaaggatgc ctttttctgc ctgagactcc     3660

accccaccag tcagcctctc ttcgcctttg agtggagaga tccagagatg ggaatctcag     3720

gacaattgac ctggaccaga ctcccacagg gtttcaaaaa cagtcccacc ctgtttgatg     3780

aggcactgca cagagaccta gcagacttcc ggatccagca cccagacttg atcctgctac     3840

agtacgtgga tgacttactg ctggccgcca cttctgagct agactgccaa caaggtactc     3900

gggccctgtt acaaacccta gggaacctcg ggtatcgggc ctcggccaag aaagcccaaa     3960

tttgccagaa acaggtcaag tatctggggt atcttctaaa agagggtcag agatggctga     4020

ctgaggccag aaaagagact gtgatggggc agcctactcc gaagacccct cgacaactaa     4080

gggagttcct agggacggca ggcttctgtc gcctctggat ccctgggttt gcagaaatgg     4140

cagccccctt gtaccctctc accaaaacgg ggactctgtt taattggggc ccagaccaac     4200

aaaaggccta tcaagaaatc aagcaagctc ttctaactgc cccagccctg gggttgccag     4260

atttgactaa gccctttgaa ctctttgtcg acgagaagca gggctacgcc aaaggtgtcc     4320

taacgcaaaa actgggacct tggcgtcggc cggtggccta cctgtccaaa aagctagacc     4380

cagtagcagc tgggtggccc ccttgcctac ggatggtagc agccattgcc gtactgacaa     4440

aggatgcagg caagctaacc atgggacagc cactagtcat tctggccccc catgcagtag     4500

aggcactagt caaacaaccc cccgaccgct ggctttccaa cgcccggatg actcactatc     4560

aggccttgct tttggacacg gaccgggtcc agttcggacc ggtggtagcc ctgaacccgg     4620

ctacgctgct cccactgcct gaggaagggc tgcaacacaa ctgccttgat atcctggccg     4680

aagcccacgg aacccgaccc gacctaacgg accagccgct cccagacgcc gaccacacct     4740

ggtacacgga tggaagcagt ctcttacaag agggacagcg taaggcggga gctgcggtga     4800

ccaccgagac cgaggtaatc tgggctaaag ccctgccagc cgggacatcc gctcagcggg     4860

ctgaactgat agcactcacc caggccctaa agatggcaga aggtaagaag ctaaatgttt     4920

atactgatag ccgttatgct tttgctactg cccatatcca tggagaaata tacagaaggc     4980

gtgggttgct cacatcagaa ggcaaagaga tcaaaaataa agacgagatc ttggccctac     5040

taaaagccct ctttctgccc aaaagactta gcataatcca ttgtccagga catcaaaagg     5100

gacacagcgc cgaggctaga ggcaaccgga tggctgacca agcggcccga aaggcagcca     5160

tcacagagac tccagacacc tctaccctcc tcatagaaaa ttcatcaccc tacacctcag     5220

aacattttca ttacacagtg actgatataa aggacctaac caagttgggg gccatttatg     5280

ataaaacaaa gaagtattgg gtctaccaag gaaaacctgt gatgcctgac cagtttactt     5340

ttgaattatt agactttctt catcagctga ctcacctcag cttctcaaaa atgaaggctc     5400

tcctagagag aagccacagt ccctactaca tgctgaaccg ggatcgaaca ctcaaaaata     5460

tcactgagac ctgcaaagct tgtgcacaag tcaacgccag caagtctgcc gttaaacagg     5520

gaactagggt ccgcgggcat cggcccggca ctcattggga gatcgatttc accgagataa     5580

agcccggatt gtatggctat aaatatcttc tagtttttat agataccttt tctggctgga     5640

tagaagcctt cccaaccaag aaagaaaccg ccaaggtcgt aaccaagaag ctactagagg     5700

agatcttccc caggttcggc atgcctcagg tattgggaac tgacaatggg cctgccttcg     5760

tctccaaggt gagtcagaca gtggccgatc tgttggggat tgattggaaa ttacattgtg     5820

catacagacc ccaaagctca ggccaggtag aaagaatgaa tagaaccatc aaggagactt     5880

taactaaatt aacgcttgca actggctcta gagactgggt gctcctactc cccttagccc     5940

tgtaccgagc ccgcaacacg ccgggccccc atggcctcac cccatatgag atcttatatg     6000

gggcaccccc gccccttgta aacttccctg accctgacat gacaagagtt actaacagcc     6060

cctctctcca agctcactta caggctctct acttagtcca gcacgaagtc tggagacctc     6120

tggcggcagc ctaccaagaa caactggacc gaccggtggt acctcaccct taccgagtcg     6180

gcgacacagt gtgggtccgc cgacaccaga ctaagaacct agaacctcgc tggaaaggac     6240

cttacacagt cctgctgacc acccccaccg ccctcaaagt agacggcatc gcagcttgga     6300

tacacgccgc ccacgtgaag gctgccgacc ccgggggtgg accatcctct agactgacat     6360

ggcgcgttca acgctctcaa aaccccctca agataagatt aacccgtgga agcccttaat     6420

agtcatggga gtcctgttag gagtagggat ggcagagagc ccccatcagg tctttaatgt     6480

aacctggaga gtcaccaacc tgatgactgg gcgtaccgcc aatgccacct ccctcctggg     6540

aactgtacaa gatgccttcc caaaattata ttttgatcta tgtgatctgg tcggagagga     6600

gtgggaccct tcagaccagg aaccgtatgt cgggtatggc tgcaagtacc ccgcagggag     6660

acagcggacc cggacttttg acttttacgt gtgccctggg cataccgtaa agtcggggtg     6720

tgggggacca ggagagggct actgtggtaa atgggggtgt gaaaccaccg gacaggctta     6780

ctggaagccc acatcatcgt gggacctaat ctcccttaag cgcggtaaca ccccctggga     6840

cacgggatgc tctaaagttg cctgtggccc ctgctacgac ctctccaaag tatccaattc     6900

cttccaaggg gctactcgag ggggcagatg caaccctcta gtcctagaat tcactgatgc     6960

aggaaaaaag gctaactggg acgggcccaa atcgtgggga ctgagactgt accggacagg     7020

aacagatcct attaccatgt tctccctgac ccggcaggtc cttaatgtgg gaccccgagt     7080

ccccataggg cccaacccag tattacccga ccaaagactc ccttcctcac caatagagat     7140

tgtaccggct ccacagccac ctagccccct caataccagt tacccccctt ccactaccag     7200

tacaccctca acctccccta caagtccaag tgtcccacag ccacccccag gaactggaga     7260

tagactacta gctctagtca aaggagccta tcaggcgctt aacctcacca atcccgacaa     7320

gacccaagaa tgttggctgt gcttagtgtc gggacctcct tattacgaag gagtagcggt     7380

cgtgggcact tataccaatc attccaccgc tccggccaac tgtacggcca cttcccaaca     7440

taagcttacc ctatctgaag tgacaggaca gggcctatgc atgggggcag tacctaaaac     7500

tcaccaggcc ttatgtaaca ccacccaaag cgccggctca ggatcctact accttgcagc     7560

acccgccgga acaatgtggg cttgcagcac tggattgact ccctgcttgt ccaccacggt     7620

gctcaatcta accacagatt attgtgtatt agttgaactc tggcccagag taatttacca     7680

ctcccccgat tatatgtatg gtcagcttga acagcgtacc aaatataaaa gagagccagt     7740

atcattgacc ctggcccttc tactaggagg attaaccatg ggagggattg cagctggaat     7800

agggacgggg accactgcct taattaaaac ccagcagttt gagcagcttc atgccgctat     7860

ccagacagac ctcaacgaag tcgaaaagtc aattaccaac ctagaaaagt cactgacctc     7920

gttgtctgaa gtagtcctac agaaccgcag aggcctagat ttgctattcc taaaggaggg     7980

aggtctctgc gcagccctaa aagaagaatg ttgtttttat gcagaccaca cggggctagt     8040

gagagacagc atggccaaat taagagaaag gcttaatcag agacaaaaac tatttgagac     8100

aggccaagga tggttcgaag ggctgtttaa tagatccccc tggtttacca ccttaatctc     8160

caccatcatg ggacctctaa tagtactctt actgatctta ctctttggac cttgcattct     8220

caatcgattg gtccaatttg ttaaagacag gatctcagtg gtccaggctc tggttttgac     8280

tcagcaatat caccagctaa aacccataga gtacgagcca tgaacgcgta atgtagtctt     8340

atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag     8400

gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat     8460

taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattc cgcattgcag     8520

agatattgta tttaagtgcc tagctcgata caataaacgc catttgacca ttcaccacat     8580

tggtgtgcac cggatcctgc tcgcttcggc agcacatata ctagtcgact agggataaca     8640

gggtaattgt ttgaatgagg cttcagtact ttacagaatc gttgcctgca catcttggaa     8700

acacttgctg ggattacttc ttcaggttaa cccaacagaa ggctcgagaa ggtatattgc     8760

tgttgacagt gagcgcggta gcaatatgac aattgatagt gaagccacag atgtatcaat     8820

tgtcatattg ctaccatgcc tactgcctcg gaattcaagg ggctacttta ggagcaatta     8880

tcttgtttac taaaactgaa taccttgcta tctctttgat acatttttac aaagctgaat     8940

taaaatggta taaattaaat cacttttttc aattggaaga ctaatgcggc ggccgcagat     9000

aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag     9060

gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag     9120

aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag     9180

gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga     9240

atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac     9300

agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc     9360

agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc     9420

ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct     9480

cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa     9540

ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag     9600

tgattgacta cccgtcagcg ggggtctttc attacatgtg agcaaaaggc cagcaaaagg     9660

ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg     9720

agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat     9780

accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta     9840

ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct     9900

gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc     9960

ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa    10020

gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg    10080

taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag    10140

tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt    10200

gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta    10260

cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc    10320

agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca    10380

cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa    10440

cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat    10500

ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct    10560

taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt    10620

tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat    10680

ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta    10740

atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg    10800

gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt    10860

tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg    10920

cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg    10980

taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc    11040

ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa    11100

ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac    11160

cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt    11220

ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg    11280

gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa    11340

gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata    11400

aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca    11460

ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag    11520

aattccat                                                             11528


<210>  15
<211>  12008
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector pAC3-RSV-yCD2-PDL1miR30shRNA4

<400>  15
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cggcgccagt cctccgattg      600

actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt      660

ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc      720

tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca      780

ccgggaggta agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac      840

tgattttatg cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg      900

tggtggaact gacgagttcg gaacacccgg ccgcaaccct gggagacgtc ccagggactt      960

cgggggccgt ttttgtggcc cgacctgagt ccaaaaatcc cgatcgtttt ggactctttg     1020

gtgcaccccc cttagaggag ggatatgtgg ttctggtagg agacgagaac ctaaaacagt     1080

tcccgcctcc gtctgaattt ttgctttcgg tttgggaccg aagccgcgcc gcgcgtcttg     1140

tctgctgcag catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa     1200

atatgggcca gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg     1260

agcggatcgc tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct     1320

ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc     1380

tcatcaccca ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg     1440

tcccctacat cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct     1500

ttgtacaccc taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac     1560

ctcctcgttc gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg     1620

ccaaacctaa acctcaagtt ctttctgaca gtggggggcc gctcatcgac ctacttacag     1680

aagacccccc gccttatagg gacccaagac cacccccttc cgacagggac ggaaatggtg     1740

gagaagcgac ccctgcggga gaggcaccgg acccctcccc aatggcatct cgcctacgtg     1800

ggagacggga gccccctgtg gccgactcca ctacctcgca ggcattcccc ctccgcgcag     1860

gaggaaacgg acagcttcaa tactggccgt tctcctcttc tgacctttac aactggaaaa     1920

ataataaccc ttctttttct gaagatccag gtaaactgac agctctgatc gagtctgtcc     1980

tcatcaccca tcagcccacc tgggacgact gtcagcagct gttggggact ctgctgaccg     2040

gagaagaaaa acaacgggtg ctcttagagg ctagaaaggc ggtgcggggc gatgatgggc     2100

gccccactca actgcccaat gaagtcgatg ccgcttttcc cctcgagcgc ccagactggg     2160

attacaccac ccaggcaggt aggaaccacc tagtccacta tcgccagttg ctcctagcgg     2220

gtctccaaaa cgcgggcaga agccccacca atttggccaa ggtaaaagga ataacacaag     2280

ggcccaatga gtctccctcg gccttcctag agagacttaa ggaagcctat cgcaggtaca     2340

ctccttatga ccctgaggac ccagggcaag aaactaatgt gtctatgtct ttcatttggc     2400

agtctgcccc agacattggg agaaagttag agaggttaga agatttaaaa aacaagacgc     2460

ttggagattt ggttagagag gcagaaaaga tctttaataa acgagaaacc ccggaagaaa     2520

gagaggaacg tatcaggaga gaaacagagg aaaaagaaga acgccgtagg acagaggatg     2580

agcagaaaga gaaagaaaga gatcgtagga gacatagaga gatgagcaag ctattggcca     2640

ctgtcgttag tggacagaaa caggatagac agggaggaga acgaaggagg tcccaactcg     2700

atcgcgacca gtgtgcctac tgcaaagaaa aggggcactg ggctaaagat tgtcccaaga     2760

aaccacgagg acctcgggga ccaagacccc agacctccct cctgacccta gatgactagg     2820

gaggtcaggg tcaggagccc ccccctgaac ccaggataac cctcaaagtc ggggggcaac     2880

ccgtcacctt cctggtagat actggggccc aacactccgt gctgacccaa aatcctggac     2940

ccctaagtga taagtctgcc tgggtccaag gggctactgg aggaaagcgg tatcgctgga     3000

ccacggatcg caaagtacat ctagctaccg gtaaggtcac ccactctttc ctccatgtac     3060

cagactgtcc ctatcctctg ttaggaagag atttgctgac taaactaaaa gcccaaatcc     3120

actttgaggg atcaggagcc caggttatgg gaccaatggg gcagcccctg caagtgttga     3180

ccctaaatat agaagatgag tatcggctac atgagacctc aaaagagcca gatgtttctc     3240

tagggtccac atggctgtct gattttcctc aggcctgggc ggaaaccggg ggcatgggac     3300

tggcagttcg ccaagctcct ctgatcatac ctctgaaagc aacctctacc cccgtgtcca     3360

taaaacaata ccccatgtca caagaagcca gactggggat caagccccac atacagagac     3420

tgttggacca gggaatactg gtaccctgcc agtccccctg gaacacgccc ctgctacccg     3480

ttaagaaacc agggactaat gattataggc ctgtccagga tctgagagaa gtcaacaagc     3540

gggtggaaga catccacccc accgtgccca acccttacaa cctcttgagc gggctcccac     3600

cgtcccacca gtggtacact gtgcttgatt taaaggatgc ctttttctgc ctgagactcc     3660

accccaccag tcagcctctc ttcgcctttg agtggagaga tccagagatg ggaatctcag     3720

gacaattgac ctggaccaga ctcccacagg gtttcaaaaa cagtcccacc ctgtttgatg     3780

aggcactgca cagagaccta gcagacttcc ggatccagca cccagacttg atcctgctac     3840

agtacgtgga tgacttactg ctggccgcca cttctgagct agactgccaa caaggtactc     3900

gggccctgtt acaaacccta gggaacctcg ggtatcgggc ctcggccaag aaagcccaaa     3960

tttgccagaa acaggtcaag tatctggggt atcttctaaa agagggtcag agatggctga     4020

ctgaggccag aaaagagact gtgatggggc agcctactcc gaagacccct cgacaactaa     4080

gggagttcct agggacggca ggcttctgtc gcctctggat ccctgggttt gcagaaatgg     4140

cagccccctt gtaccctctc accaaaacgg ggactctgtt taattggggc ccagaccaac     4200

aaaaggccta tcaagaaatc aagcaagctc ttctaactgc cccagccctg gggttgccag     4260

atttgactaa gccctttgaa ctctttgtcg acgagaagca gggctacgcc aaaggtgtcc     4320

taacgcaaaa actgggacct tggcgtcggc cggtggccta cctgtccaaa aagctagacc     4380

cagtagcagc tgggtggccc ccttgcctac ggatggtagc agccattgcc gtactgacaa     4440

aggatgcagg caagctaacc atgggacagc cactagtcat tctggccccc catgcagtag     4500

aggcactagt caaacaaccc cccgaccgct ggctttccaa cgcccggatg actcactatc     4560

aggccttgct tttggacacg gaccgggtcc agttcggacc ggtggtagcc ctgaacccgg     4620

ctacgctgct cccactgcct gaggaagggc tgcaacacaa ctgccttgat atcctggccg     4680

aagcccacgg aacccgaccc gacctaacgg accagccgct cccagacgcc gaccacacct     4740

ggtacacgga tggaagcagt ctcttacaag agggacagcg taaggcggga gctgcggtga     4800

ccaccgagac cgaggtaatc tgggctaaag ccctgccagc cgggacatcc gctcagcggg     4860

ctgaactgat agcactcacc caggccctaa agatggcaga aggtaagaag ctaaatgttt     4920

atactgatag ccgttatgct tttgctactg cccatatcca tggagaaata tacagaaggc     4980

gtgggttgct cacatcagaa ggcaaagaga tcaaaaataa agacgagatc ttggccctac     5040

taaaagccct ctttctgccc aaaagactta gcataatcca ttgtccagga catcaaaagg     5100

gacacagcgc cgaggctaga ggcaaccgga tggctgacca agcggcccga aaggcagcca     5160

tcacagagac tccagacacc tctaccctcc tcatagaaaa ttcatcaccc tacacctcag     5220

aacattttca ttacacagtg actgatataa aggacctaac caagttgggg gccatttatg     5280

ataaaacaaa gaagtattgg gtctaccaag gaaaacctgt gatgcctgac cagtttactt     5340

ttgaattatt agactttctt catcagctga ctcacctcag cttctcaaaa atgaaggctc     5400

tcctagagag aagccacagt ccctactaca tgctgaaccg ggatcgaaca ctcaaaaata     5460

tcactgagac ctgcaaagct tgtgcacaag tcaacgccag caagtctgcc gttaaacagg     5520

gaactagggt ccgcgggcat cggcccggca ctcattggga gatcgatttc accgagataa     5580

agcccggatt gtatggctat aaatatcttc tagtttttat agataccttt tctggctgga     5640

tagaagcctt cccaaccaag aaagaaaccg ccaaggtcgt aaccaagaag ctactagagg     5700

agatcttccc caggttcggc atgcctcagg tattgggaac tgacaatggg cctgccttcg     5760

tctccaaggt gagtcagaca gtggccgatc tgttggggat tgattggaaa ttacattgtg     5820

catacagacc ccaaagctca ggccaggtag aaagaatgaa tagaaccatc aaggagactt     5880

taactaaatt aacgcttgca actggctcta gagactgggt gctcctactc cccttagccc     5940

tgtaccgagc ccgcaacacg ccgggccccc atggcctcac cccatatgag atcttatatg     6000

gggcaccccc gccccttgta aacttccctg accctgacat gacaagagtt actaacagcc     6060

cctctctcca agctcactta caggctctct acttagtcca gcacgaagtc tggagacctc     6120

tggcggcagc ctaccaagaa caactggacc gaccggtggt acctcaccct taccgagtcg     6180

gcgacacagt gtgggtccgc cgacaccaga ctaagaacct agaacctcgc tggaaaggac     6240

cttacacagt cctgctgacc acccccaccg ccctcaaagt agacggcatc gcagcttgga     6300

tacacgccgc ccacgtgaag gctgccgacc ccgggggtgg accatcctct agactgacat     6360

ggcgcgttca acgctctcaa aaccccctca agataagatt aacccgtgga agcccttaat     6420

agtcatggga gtcctgttag gagtagggat ggcagagagc ccccatcagg tctttaatgt     6480

aacctggaga gtcaccaacc tgatgactgg gcgtaccgcc aatgccacct ccctcctggg     6540

aactgtacaa gatgccttcc caaaattata ttttgatcta tgtgatctgg tcggagagga     6600

gtgggaccct tcagaccagg aaccgtatgt cgggtatggc tgcaagtacc ccgcagggag     6660

acagcggacc cggacttttg acttttacgt gtgccctggg cataccgtaa agtcggggtg     6720

tgggggacca ggagagggct actgtggtaa atgggggtgt gaaaccaccg gacaggctta     6780

ctggaagccc acatcatcgt gggacctaat ctcccttaag cgcggtaaca ccccctggga     6840

cacgggatgc tctaaagttg cctgtggccc ctgctacgac ctctccaaag tatccaattc     6900

cttccaaggg gctactcgag ggggcagatg caaccctcta gtcctagaat tcactgatgc     6960

aggaaaaaag gctaactggg acgggcccaa atcgtgggga ctgagactgt accggacagg     7020

aacagatcct attaccatgt tctccctgac ccggcaggtc cttaatgtgg gaccccgagt     7080

ccccataggg cccaacccag tattacccga ccaaagactc ccttcctcac caatagagat     7140

tgtaccggct ccacagccac ctagccccct caataccagt tacccccctt ccactaccag     7200

tacaccctca acctccccta caagtccaag tgtcccacag ccacccccag gaactggaga     7260

tagactacta gctctagtca aaggagccta tcaggcgctt aacctcacca atcccgacaa     7320

gacccaagaa tgttggctgt gcttagtgtc gggacctcct tattacgaag gagtagcggt     7380

cgtgggcact tataccaatc attccaccgc tccggccaac tgtacggcca cttcccaaca     7440

taagcttacc ctatctgaag tgacaggaca gggcctatgc atgggggcag tacctaaaac     7500

tcaccaggcc ttatgtaaca ccacccaaag cgccggctca ggatcctact accttgcagc     7560

acccgccgga acaatgtggg cttgcagcac tggattgact ccctgcttgt ccaccacggt     7620

gctcaatcta accacagatt attgtgtatt agttgaactc tggcccagag taatttacca     7680

ctcccccgat tatatgtatg gtcagcttga acagcgtacc aaatataaaa gagagccagt     7740

atcattgacc ctggcccttc tactaggagg attaaccatg ggagggattg cagctggaat     7800

agggacgggg accactgcct taattaaaac ccagcagttt gagcagcttc atgccgctat     7860

ccagacagac ctcaacgaag tcgaaaagtc aattaccaac ctagaaaagt cactgacctc     7920

gttgtctgaa gtagtcctac agaaccgcag aggcctagat ttgctattcc taaaggaggg     7980

aggtctctgc gcagccctaa aagaagaatg ttgtttttat gcagaccaca cggggctagt     8040

gagagacagc atggccaaat taagagaaag gcttaatcag agacaaaaac tatttgagac     8100

aggccaagga tggttcgaag ggctgtttaa tagatccccc tggtttacca ccttaatctc     8160

caccatcatg ggacctctaa tagtactctt actgatctta ctctttggac cttgcattct     8220

caatcgattg gtccaatttg ttaaagacag gatctcagtg gtccaggctc tggttttgac     8280

tcagcaatat caccagctaa aacccataga gtacgagcca tgaacgcgta atgtagtctt     8340

atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag     8400

gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat     8460

taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattc cgcattgcag     8520

agatattgta tttaagtgcc tagctcgata caataaacgc catttgacca ttcaccacat     8580

tggtgtgcac catggtgacc ggcggcatgg cctccaagtg ggatcaaaag ggcatggata     8640

tcgcttacga ggaggccctg ctgggctaca aggagggcgg cgtgcctatc ggcggctgtc     8700

tgatcaacaa caaggacggc agtgtgctgg gcaggggcca caacatgagg ttccagaagg     8760

gctccgccac cctgcacggc gagatctcca ccctggagaa ctgtggcagg ctggagggca     8820

aggtgtacaa ggacaccacc ctgtacacca ccctgtcccc ttgtgacatg tgtaccggcg     8880

ctatcatcat gtacggcatc cctaggtgtg tgatcggcga gaacgtgaac ttcaagtcca     8940

agggcgagaa gtacctgcaa accaggggcc acgaggtggt ggttgttgac gatgagaggt     9000

gtaagaagct gatgaagcag ttcatcgacg agaggcctca ggactggttc gaggatatcg     9060

gcgagtaatg aggatcctgc tcgcttcggc agcacatata ctagtcgact agggataaca     9120

gggtaattgt ttgaatgagg cttcagtact ttacagaatc gttgcctgca catcttggaa     9180

acacttgctg ggattacttc ttcaggttaa cccaacagaa ggctcgagaa ggtatattgc     9240

tgttgacagt gagcgcggta gcaatatgac aattgatagt gaagccacag atgtatcaat     9300

tgtcatattg ctaccatgcc tactgcctcg gaattcaagg ggctacttta ggagcaatta     9360

tcttgtttac taaaactgaa taccttgcta tctctttgat acatttttac aaagctgaat     9420

taaaatggta taaattaaat cacttttttc aattggaaga ctaatgcggc ggccgcagat     9480

aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag     9540

gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag     9600

aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag     9660

gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga     9720

atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac     9780

agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc     9840

agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc     9900

ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct     9960

cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa    10020

ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag    10080

tgattgacta cccgtcagcg ggggtctttc attacatgtg agcaaaaggc cagcaaaagg    10140

ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg    10200

agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat    10260

accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta    10320

ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct    10380

gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc    10440

ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa    10500

gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg    10560

taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag    10620

tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt    10680

gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta    10740

cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc    10800

agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca    10860

cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa    10920

cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat    10980

ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct    11040

taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt    11100

tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat    11160

ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta    11220

atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg    11280

gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt    11340

tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg    11400

cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg    11460

taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc    11520

ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa    11580

ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac    11640

cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt    11700

ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg    11760

gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa    11820

gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata    11880

aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca    11940

ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag    12000

aattccat                                                             12008


<210>  16
<211>  12273
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Vector pAC3-RSV-yCD2-U6-PDL1miR30shRNA4

<400>  16
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cggcgccagt cctccgattg      600

actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt      660

ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc      720

tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca      780

ccgggaggta agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac      840

tgattttatg cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg      900

tggtggaact gacgagttcg gaacacccgg ccgcaaccct gggagacgtc ccagggactt      960

cgggggccgt ttttgtggcc cgacctgagt ccaaaaatcc cgatcgtttt ggactctttg     1020

gtgcaccccc cttagaggag ggatatgtgg ttctggtagg agacgagaac ctaaaacagt     1080

tcccgcctcc gtctgaattt ttgctttcgg tttgggaccg aagccgcgcc gcgcgtcttg     1140

tctgctgcag catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa     1200

atatgggcca gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg     1260

agcggatcgc tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct     1320

ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc     1380

tcatcaccca ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg     1440

tcccctacat cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct     1500

ttgtacaccc taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac     1560

ctcctcgttc gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg     1620

ccaaacctaa acctcaagtt ctttctgaca gtggggggcc gctcatcgac ctacttacag     1680

aagacccccc gccttatagg gacccaagac cacccccttc cgacagggac ggaaatggtg     1740

gagaagcgac ccctgcggga gaggcaccgg acccctcccc aatggcatct cgcctacgtg     1800

ggagacggga gccccctgtg gccgactcca ctacctcgca ggcattcccc ctccgcgcag     1860

gaggaaacgg acagcttcaa tactggccgt tctcctcttc tgacctttac aactggaaaa     1920

ataataaccc ttctttttct gaagatccag gtaaactgac agctctgatc gagtctgtcc     1980

tcatcaccca tcagcccacc tgggacgact gtcagcagct gttggggact ctgctgaccg     2040

gagaagaaaa acaacgggtg ctcttagagg ctagaaaggc ggtgcggggc gatgatgggc     2100

gccccactca actgcccaat gaagtcgatg ccgcttttcc cctcgagcgc ccagactggg     2160

attacaccac ccaggcaggt aggaaccacc tagtccacta tcgccagttg ctcctagcgg     2220

gtctccaaaa cgcgggcaga agccccacca atttggccaa ggtaaaagga ataacacaag     2280

ggcccaatga gtctccctcg gccttcctag agagacttaa ggaagcctat cgcaggtaca     2340

ctccttatga ccctgaggac ccagggcaag aaactaatgt gtctatgtct ttcatttggc     2400

agtctgcccc agacattggg agaaagttag agaggttaga agatttaaaa aacaagacgc     2460

ttggagattt ggttagagag gcagaaaaga tctttaataa acgagaaacc ccggaagaaa     2520

gagaggaacg tatcaggaga gaaacagagg aaaaagaaga acgccgtagg acagaggatg     2580

agcagaaaga gaaagaaaga gatcgtagga gacatagaga gatgagcaag ctattggcca     2640

ctgtcgttag tggacagaaa caggatagac agggaggaga acgaaggagg tcccaactcg     2700

atcgcgacca gtgtgcctac tgcaaagaaa aggggcactg ggctaaagat tgtcccaaga     2760

aaccacgagg acctcgggga ccaagacccc agacctccct cctgacccta gatgactagg     2820

gaggtcaggg tcaggagccc ccccctgaac ccaggataac cctcaaagtc ggggggcaac     2880

ccgtcacctt cctggtagat actggggccc aacactccgt gctgacccaa aatcctggac     2940

ccctaagtga taagtctgcc tgggtccaag gggctactgg aggaaagcgg tatcgctgga     3000

ccacggatcg caaagtacat ctagctaccg gtaaggtcac ccactctttc ctccatgtac     3060

cagactgtcc ctatcctctg ttaggaagag atttgctgac taaactaaaa gcccaaatcc     3120

actttgaggg atcaggagcc caggttatgg gaccaatggg gcagcccctg caagtgttga     3180

ccctaaatat agaagatgag tatcggctac atgagacctc aaaagagcca gatgtttctc     3240

tagggtccac atggctgtct gattttcctc aggcctgggc ggaaaccggg ggcatgggac     3300

tggcagttcg ccaagctcct ctgatcatac ctctgaaagc aacctctacc cccgtgtcca     3360

taaaacaata ccccatgtca caagaagcca gactggggat caagccccac atacagagac     3420

tgttggacca gggaatactg gtaccctgcc agtccccctg gaacacgccc ctgctacccg     3480

ttaagaaacc agggactaat gattataggc ctgtccagga tctgagagaa gtcaacaagc     3540

gggtggaaga catccacccc accgtgccca acccttacaa cctcttgagc gggctcccac     3600

cgtcccacca gtggtacact gtgcttgatt taaaggatgc ctttttctgc ctgagactcc     3660

accccaccag tcagcctctc ttcgcctttg agtggagaga tccagagatg ggaatctcag     3720

gacaattgac ctggaccaga ctcccacagg gtttcaaaaa cagtcccacc ctgtttgatg     3780

aggcactgca cagagaccta gcagacttcc ggatccagca cccagacttg atcctgctac     3840

agtacgtgga tgacttactg ctggccgcca cttctgagct agactgccaa caaggtactc     3900

gggccctgtt acaaacccta gggaacctcg ggtatcgggc ctcggccaag aaagcccaaa     3960

tttgccagaa acaggtcaag tatctggggt atcttctaaa agagggtcag agatggctga     4020

ctgaggccag aaaagagact gtgatggggc agcctactcc gaagacccct cgacaactaa     4080

gggagttcct agggacggca ggcttctgtc gcctctggat ccctgggttt gcagaaatgg     4140

cagccccctt gtaccctctc accaaaacgg ggactctgtt taattggggc ccagaccaac     4200

aaaaggccta tcaagaaatc aagcaagctc ttctaactgc cccagccctg gggttgccag     4260

atttgactaa gccctttgaa ctctttgtcg acgagaagca gggctacgcc aaaggtgtcc     4320

taacgcaaaa actgggacct tggcgtcggc cggtggccta cctgtccaaa aagctagacc     4380

cagtagcagc tgggtggccc ccttgcctac ggatggtagc agccattgcc gtactgacaa     4440

aggatgcagg caagctaacc atgggacagc cactagtcat tctggccccc catgcagtag     4500

aggcactagt caaacaaccc cccgaccgct ggctttccaa cgcccggatg actcactatc     4560

aggccttgct tttggacacg gaccgggtcc agttcggacc ggtggtagcc ctgaacccgg     4620

ctacgctgct cccactgcct gaggaagggc tgcaacacaa ctgccttgat atcctggccg     4680

aagcccacgg aacccgaccc gacctaacgg accagccgct cccagacgcc gaccacacct     4740

ggtacacgga tggaagcagt ctcttacaag agggacagcg taaggcggga gctgcggtga     4800

ccaccgagac cgaggtaatc tgggctaaag ccctgccagc cgggacatcc gctcagcggg     4860

ctgaactgat agcactcacc caggccctaa agatggcaga aggtaagaag ctaaatgttt     4920

atactgatag ccgttatgct tttgctactg cccatatcca tggagaaata tacagaaggc     4980

gtgggttgct cacatcagaa ggcaaagaga tcaaaaataa agacgagatc ttggccctac     5040

taaaagccct ctttctgccc aaaagactta gcataatcca ttgtccagga catcaaaagg     5100

gacacagcgc cgaggctaga ggcaaccgga tggctgacca agcggcccga aaggcagcca     5160

tcacagagac tccagacacc tctaccctcc tcatagaaaa ttcatcaccc tacacctcag     5220

aacattttca ttacacagtg actgatataa aggacctaac caagttgggg gccatttatg     5280

ataaaacaaa gaagtattgg gtctaccaag gaaaacctgt gatgcctgac cagtttactt     5340

ttgaattatt agactttctt catcagctga ctcacctcag cttctcaaaa atgaaggctc     5400

tcctagagag aagccacagt ccctactaca tgctgaaccg ggatcgaaca ctcaaaaata     5460

tcactgagac ctgcaaagct tgtgcacaag tcaacgccag caagtctgcc gttaaacagg     5520

gaactagggt ccgcgggcat cggcccggca ctcattggga gatcgatttc accgagataa     5580

agcccggatt gtatggctat aaatatcttc tagtttttat agataccttt tctggctgga     5640

tagaagcctt cccaaccaag aaagaaaccg ccaaggtcgt aaccaagaag ctactagagg     5700

agatcttccc caggttcggc atgcctcagg tattgggaac tgacaatggg cctgccttcg     5760

tctccaaggt gagtcagaca gtggccgatc tgttggggat tgattggaaa ttacattgtg     5820

catacagacc ccaaagctca ggccaggtag aaagaatgaa tagaaccatc aaggagactt     5880

taactaaatt aacgcttgca actggctcta gagactgggt gctcctactc cccttagccc     5940

tgtaccgagc ccgcaacacg ccgggccccc atggcctcac cccatatgag atcttatatg     6000

gggcaccccc gccccttgta aacttccctg accctgacat gacaagagtt actaacagcc     6060

cctctctcca agctcactta caggctctct acttagtcca gcacgaagtc tggagacctc     6120

tggcggcagc ctaccaagaa caactggacc gaccggtggt acctcaccct taccgagtcg     6180

gcgacacagt gtgggtccgc cgacaccaga ctaagaacct agaacctcgc tggaaaggac     6240

cttacacagt cctgctgacc acccccaccg ccctcaaagt agacggcatc gcagcttgga     6300

tacacgccgc ccacgtgaag gctgccgacc ccgggggtgg accatcctct agactgacat     6360

ggcgcgttca acgctctcaa aaccccctca agataagatt aacccgtgga agcccttaat     6420

agtcatggga gtcctgttag gagtagggat ggcagagagc ccccatcagg tctttaatgt     6480

aacctggaga gtcaccaacc tgatgactgg gcgtaccgcc aatgccacct ccctcctggg     6540

aactgtacaa gatgccttcc caaaattata ttttgatcta tgtgatctgg tcggagagga     6600

gtgggaccct tcagaccagg aaccgtatgt cgggtatggc tgcaagtacc ccgcagggag     6660

acagcggacc cggacttttg acttttacgt gtgccctggg cataccgtaa agtcggggtg     6720

tgggggacca ggagagggct actgtggtaa atgggggtgt gaaaccaccg gacaggctta     6780

ctggaagccc acatcatcgt gggacctaat ctcccttaag cgcggtaaca ccccctggga     6840

cacgggatgc tctaaagttg cctgtggccc ctgctacgac ctctccaaag tatccaattc     6900

cttccaaggg gctactcgag ggggcagatg caaccctcta gtcctagaat tcactgatgc     6960

aggaaaaaag gctaactggg acgggcccaa atcgtgggga ctgagactgt accggacagg     7020

aacagatcct attaccatgt tctccctgac ccggcaggtc cttaatgtgg gaccccgagt     7080

ccccataggg cccaacccag tattacccga ccaaagactc ccttcctcac caatagagat     7140

tgtaccggct ccacagccac ctagccccct caataccagt tacccccctt ccactaccag     7200

tacaccctca acctccccta caagtccaag tgtcccacag ccacccccag gaactggaga     7260

tagactacta gctctagtca aaggagccta tcaggcgctt aacctcacca atcccgacaa     7320

gacccaagaa tgttggctgt gcttagtgtc gggacctcct tattacgaag gagtagcggt     7380

cgtgggcact tataccaatc attccaccgc tccggccaac tgtacggcca cttcccaaca     7440

taagcttacc ctatctgaag tgacaggaca gggcctatgc atgggggcag tacctaaaac     7500

tcaccaggcc ttatgtaaca ccacccaaag cgccggctca ggatcctact accttgcagc     7560

acccgccgga acaatgtggg cttgcagcac tggattgact ccctgcttgt ccaccacggt     7620

gctcaatcta accacagatt attgtgtatt agttgaactc tggcccagag taatttacca     7680

ctcccccgat tatatgtatg gtcagcttga acagcgtacc aaatataaaa gagagccagt     7740

atcattgacc ctggcccttc tactaggagg attaaccatg ggagggattg cagctggaat     7800

agggacgggg accactgcct taattaaaac ccagcagttt gagcagcttc atgccgctat     7860

ccagacagac ctcaacgaag tcgaaaagtc aattaccaac ctagaaaagt cactgacctc     7920

gttgtctgaa gtagtcctac agaaccgcag aggcctagat ttgctattcc taaaggaggg     7980

aggtctctgc gcagccctaa aagaagaatg ttgtttttat gcagaccaca cggggctagt     8040

gagagacagc atggccaaat taagagaaag gcttaatcag agacaaaaac tatttgagac     8100

aggccaagga tggttcgaag ggctgtttaa tagatccccc tggtttacca ccttaatctc     8160

caccatcatg ggacctctaa tagtactctt actgatctta ctctttggac cttgcattct     8220

caatcgattg gtccaatttg ttaaagacag gatctcagtg gtccaggctc tggttttgac     8280

tcagcaatat caccagctaa aacccataga gtacgagcca tgaacgcgta atgtagtctt     8340

atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag     8400

gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat     8460

taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattc cgcattgcag     8520

agatattgta tttaagtgcc tagctcgata caataaacgc catttgacca ttcaccacat     8580

tggtgtgcac catggtgacc ggcggcatgg cctccaagtg ggatcaaaag ggcatggata     8640

tcgcttacga ggaggccctg ctgggctaca aggagggcgg cgtgcctatc ggcggctgtc     8700

tgatcaacaa caaggacggc agtgtgctgg gcaggggcca caacatgagg ttccagaagg     8760

gctccgccac cctgcacggc gagatctcca ccctggagaa ctgtggcagg ctggagggca     8820

aggtgtacaa ggacaccacc ctgtacacca ccctgtcccc ttgtgacatg tgtaccggcg     8880

ctatcatcat gtacggcatc cctaggtgtg tgatcggcga gaacgtgaac ttcaagtcca     8940

agggcgagaa gtacctgcaa accaggggcc acgaggtggt ggttgttgac gatgagaggt     9000

gtaagaagct gatgaagcag ttcatcgacg agaggcctca ggactggttc gaggatatcg     9060

gcgagtaatg aaaggtcggg caggaagagg gcctatttcc catgattcct tcatatttgc     9120

atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta aacacaaaga     9180

tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttta     9240

aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc     9300

ttggctttat atatcttgtg gaaaggacga aacaccggat cctgctcgct tcggcagcac     9360

atatactagt cgactaggga taacagggta attgtttgaa tgaggcttca gtactttaca     9420

gaatcgttgc ctgcacatct tggaaacact tgctgggatt acttcttcag gttaacccaa     9480

cagaaggctc gagaaggtat attgctgttg acagtgagcg cggtagcaat atgacaattg     9540

atagtgaagc cacagatgta tcaattgtca tattgctacc atgcctactg cctcggaatt     9600

caaggggcta ctttaggagc aattatcttg tttactaaaa ctgaatacct tgctatctct     9660

ttgatacatt tttacaaagc tgaattaaaa tggtataaat taaatcactt ttttcaattg     9720

gaagactaat gcggcggccg cagataaaat aaaagatttt atttagtctc cagaaaaagg     9780

ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca     9840

aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg     9900

gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag     9960

ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt    10020

cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt    10080

ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct    10140

tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag    10200

ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg    10260

cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct    10320

gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcattac    10380

atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt    10440

ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg    10500

cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc    10560

tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc    10620

gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc    10680

aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac    10740

tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt    10800

aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct    10860

aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc    10920

ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt    10980

ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg    11040

atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc    11100

atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa    11160

tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag    11220

gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg    11280

tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga    11340

gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag    11400

cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa    11460

gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc    11520

atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca    11580

aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg    11640

atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat    11700

aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc    11760

aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg    11820

gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg    11880

gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt    11940

gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca    12000

ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata    12060

ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac    12120

atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa    12180

gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt    12240

atcacgaggc cctttcgtct tcaagaattc cat                                 12273


<210>  17
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SALI-IRES Forward Primer

<400>  17
gtacgtcgac tactggccga agccgcttgg a                                      31


<210>  18
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ClaI-yCD2-Reverse Primer

<400>  18
gtacatcgat ttactcgccg atatcctcga ac                                     32


<210>  19
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ClaI-hIL-2-Reverse Primer

<400>  19
gtacatcgat tcaagtcagt gttgagatga tg                                     32


<210>  20
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ClaI-mIL-2-Reverse Primer

<400>  20
gtacatcgat ttattgaggg cttgttgaga tg                                     32


<210>  21
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hU6-NotI Forward Primer

<400>  21
gatcgcggcc gcacgcgtaa ggtcgggcag gaa                                    33


<210>  22
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  shRNA-NotI-Reverse Primer

<400>  22
atctgcggcc gcctagaaaa aa                                                22


<210>  23
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miR30-NotI-Reverse Primer

<400>  23
gcggccgccg cattagtctt ccaattg                                           27


<210>  24
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IDO-1 siRNA sequence

<400>  24
tattctatag tcttacttg                                                    19


<210>  25
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IDO-1 siRNA sequence

<400>  25
tcaacttctt ctcgaagct                                                    19


<210>  26
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IDO-1 siRNA sequence

<400>  26
tgaaatgaca aactcacgg                                                    19


<210>  27
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDL-1 siRNA sequence

<400>  27
tagttcatgc tcagaagtg                                                    19


<210>  28
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDL-1 siRNA sequence

<400>  28
aatgctagac aatgaactg                                                    19


<210>  29
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDL-1 siRNA sequence

<400>  29
tatgcagcag taaacgcct                                                    19


<210>  30
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PDL-1 siRNA sequence

<400>  30
agtccgcacc accgtagct                                                    19


<210>  31
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TGFb2 siRNA sequence

<400>  31
ttgtggtgaa gccactcct                                                    19


<210>  32
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TGFb2 siRNA sequence

<400>  32
tctcctgcag taagtccct                                                    19


<210>  33
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TGFb2 siRNA sequence

<400>  33
aacaaacaga acacaagct                                                    19


