                         SEQUENCE LISTING

<110>  The Trustees of Columbia University in the City of New York
       Genia Technologies, Inc.
       Fuller, Carl
       Kumar, Shiv
       Ju, Jingyue
       Davis, Randall
       Chen, Roger
 
<120>  Chemical Methods For Producing Tagged Nucleotides

<130>  85625-A-PCT

<150>  US 61/969,628
<151>  2014-03-24

<160>  125   

<170>  PatentIn version 3.5

<210>  1
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthesized sequence


<220>
<221>  n
<222>  (1)..(1)
<223>  T-biotin-streptavidin

<220>
<221>  misc_feature
<222>  (11)..(13)
<223>  abasic site

<220>
<221>  misc_feature
<222>  (17)..(19)
<223>  abasic site

<400>  1
tttttttttt nnntttnnnt tttttttttt                                        30


<210>  2
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  T-biotin-streptavidin

<220>
<221>  misc_feature
<222>  (17)..(19)
<223>  abasic site

<400>  2
tttttttttt ttttttnnnt tttttttttt                                        30


<210>  3
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  T-biotin-streptavidin

<400>  3
tttttttttt tttttttttt tttttttttt                                        30


<210>  4
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  T-biotin-streptavidin

<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  T-fluorescein

<400>  4
tttttttttt tttttttttt tttttttttt                                        30


<210>  5
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'-hexyne-phosphate-cyanine 3-phosphate-T

<400>  5
tttttttttt tttttttttt tttttttttt                                        30


<210>  6
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic sequence


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'-hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc_feature
<222>  (13)..(13)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc_feature
<222>  (19)..(19)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc_feature
<222>  (21)..(21)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc_feature
<222>  (23)..(23)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  thiophosphate diester bond to following T

<400>  6
tttttttttt tttttttttt tttttttttt                                        30


<210>  7
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'-hexyne-phosphate-T

<400>  7
tttttttttt tttttttttt tttttttttt                                        30


<210>  8
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (7)..(14)
<223>  furan amidite

<400>  8
ttttttnnnn nnnntttttt tttttttttt                                        30


<210>  9
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (7)..(16)
<223>  thiophosphate diester bond to following T

<400>  9
tttttttttt tttttttttt tttttttttt                                        30


<210>  10
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (4)..(6)
<223>  furan amidite

<400>  10
tttnnntttt tttttttttt tttttttttt                                        30


<210>  11
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (8)..(10)
<223>  furan amidite

<400>  11
tttttttnnn tttttttttt tttttttttt                                        30


<210>  12
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (11)..(13)
<223>  furan amidite

<400>  12
tttttttttt nnnttttttt tttttttttt                                        30


<210>  13
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (14)..(16)
<223>  furan amidite

<400>  13
tttttttttt tttnnntttt tttttttttt                                        30


<210>  14
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  hexanol-T

<400>  14
tttttttttt tttttttttt tttttttttt                                        30


<210>  15
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  hexanol

<400>  15
tttttttttt tttttttttt tttttttttt                                        30


<210>  16
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(14)
<223>  furan amidite

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  hexanol-T

<400>  16
ttttnnnnnn nnnntttttt tttttttttt                                        30


<210>  17
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(6)
<223>  nitropyrrole

<220>
<221>  misc_feature
<222>  (11)..(12)
<223>  nitropyrrole

<220>
<221>  misc_feature
<222>  (17)..(18)
<223>  nitropyrrole

<220>
<221>  misc_feature
<222>  (23)..(24)
<223>  nitropyrrole

<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  nitropyrrole

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  nitropyrrole-propanol

<400>  17
ttttnntttt nnttttnntt ttnnttttnn                                        30


<210>  18
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(6)
<223>  nebularine

<220>
<221>  misc_feature
<222>  (11)..(12)
<223>  nebularine

<220>
<221>  misc_feature
<222>  (17)..(18)
<223>  nebularine

<220>
<221>  misc_feature
<222>  (23)..(24)
<223>  nebularine

<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  nebularine

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  nebularine-propanol

<400>  18
ttttnntttt nnttttnntt ttnnttttnn                                        30


<210>  19
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  18-atom PEG

<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  T-propanol

<400>  19
ttttnttttt tttttttttt ttttttt                                           27


<210>  20
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(6)
<223>  18-atom PEG-phosphate

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  T-propanol

<220>
<221>  misc_feature
<222>  (28)..(28)
<223>  propanol-T

<400>  20
ttttnntttt tttttttttt ttttt                                             25


<210>  21
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(6)
<223>  9-atom PEG-phosphate

<220>
<221>  misc_feature
<222>  (28)..(28)
<223>  T-propanol

<400>  21
ttttnntttt tttttttttt tttttttt                                          28


<210>  22
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (7)..(12)
<223>  heptylamine amidite

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  22
ttttttnnnn nntttttttt tttttttttt                                        30


<210>  23
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (7)..(12)
<223>  pyrrolidine amidite

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  23
ttttttnnnn nntttttttt tttttttttt                                        30


<210>  24
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (7)..(12)
<223>  aminoethyl dT amidite

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  24
ttttttnnnn nntttttttt tttttttttt                                        30


<210>  25
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  spermine

<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  T-propanol

<400>  25
ttttnttttt tttttttttt ttttttt                                           27


<210>  26
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  spermine

<220>
<221>  misc_feature
<222>  (6)..(8)
<223>  furan amidite

<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  T-propanol

<400>  26
ttttnnnntt tttttttttt ttttttt                                           27


<210>  27
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  spermine

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  fluorescein dT amidite

<220>
<221>  misc_feature
<222>  (27)..(27)
<223>  T-propanol

<400>  27
ttttnntttt tttttttttt ttttttt                                           27


<210>  28
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  28
tttttttttt tttttttttt tttttttttt                                        30


<210>  29
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3.5-T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  29
tttttttttt tttttttttt tttttttttt                                        30


<210>  30
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-cyanine-3-phosphate-T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  30
tttttttttt tttttttttt tttttttttt                                        30


<210>  31
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  cyanine 3.5-phosphate

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  31
ttttttnttt tttttttttt tttttttttt                                        30


<210>  32
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  cyanine 3-phosphate

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  32
tttttttttt nttttttttt tttttttttt                                        30


<210>  33
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (45)..(45)
<223>  T-propanol

<400>  33
ttttcggcgc gtaagcgccg tttttttttt tttttttttt ttttt                       45


<210>  34
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (7)..(14)
<223>  furan amidite

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  34
ttttttnnnn nnnntttttt tttttttttt                                        30


<210>  35
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc-feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc-feature
<222>  (1)..(1)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc-feature
<222>  (3)..(3)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc-feature
<222>  (5)..(5)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc-feature
<222>  (7)..(7)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc-feature
<222>  (9)..(9)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc-feature
<222>  (11)..(11)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc-feature
<222>  (13)..(13)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc-feature
<222>  (15)..(15)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc-feature
<222>  (17)..(17)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc-feature
<222>  (19)..(19)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc-feature
<222>  (21)..(21)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc-feature
<222>  (23)..(23)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc-feature
<222>  (25)..(25)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc-feature
<222>  (27)..(27)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc-feature
<222>  (29)..(29)
<223>  thiophosphate diester bond to following T

<220>
<221>  misc-feature
<222>  (30)..(30)
<223>  T-propanol

<400>  35
tttttttttt tttttttttt tttttttttt                                        30


<210>  36
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (1)..(29)
<223>  thiophosphate diester bond to following T

<400>  36
tttttttttt tttttttttt tttttttttt                                        30


<210>  37
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (1)..(29)
<223>  thiophosphate diester bond to next T

<400>  37
tttttttttt tttttttttt tttttttttt                                        30


<210>  38
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  38
tttttttttt tttttttttt tttttttttt                                        30


<210>  39
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  T-propanol

<400>  39
tttttttttt ttttt                                                        15


<210>  40
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  T-propanol

<400>  40
tttttttttt tttttttttt                                                   20


<210>  41
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  T-propanol

<400>  41
tttttttttt tttttttttt ttttt                                             25


<210>  42
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3- phosphate-T

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  18-atom PEG-phosphate

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  T-propanol

<400>  42
ttnttttttt tttttttttt ttttt                                             25


<210>  43
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(12)
<223>  furan amidite

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  43
ttttnnnnnn nntttttttt tttttttttt                                        30


<210>  44
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (7)..(12)
<223>  aminoethyl dT amidite

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  44
ttttttnnnn nntttttttt tttttttttt                                        30


<210>  45
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  9 atom PEG-phosphate

<220>
<221>  misc_feature
<222>  (25)..(25)
<223>  T-propanol

<400>  45
ttttnttttt tttttttttt tttttttt                                          28


<210>  46
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (2)..(4)
<223>  furan amidite

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  46
tnnntttttt tttttttttt tttttttttt                                        30


<210>  47
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(7)
<223>  furan amidite

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  47
ttttnnnttt tttttttttt tttttttttt                                        30


<210>  48
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (8)..(10)
<223>  furan amidite

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  48
tttttttnnn tttttttttt tttttttttt                                        30


<210>  49
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (11)..(13)
<223>  furan amidite

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  49
tttttttttt nnnttttttt tttttttttt                                        30


<210>  50
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(7)
<223>  fluorescein dT amidite

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  50
ttttnnnttt tttttttttt tttttttttt                                        30


<210>  51
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  fluorescein-dT-amidite

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  fluorescein-dT-amidite

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  51
ttttntnttt tttttttttt tttttttttt                                        30


<210>  52
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-spermine-phosphate-T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  52
tttttttttt tttttttttt tttttttttt                                        30


<210>  53
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-cyanine 3-phosphate-propanol

<400>  53
tttttttttt tttttttttt tttttttttt                                        30


<210>  54
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-T

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  spermine

<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  T-propanol

<400>  54
ttttttttnt tttttttttt ttttttttt                                         29


<210>  55
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (44)..(44)
<223>  T-propanol

<400>  55
ttttggttgg tgtggttggt tttttttttt tttttttttt tttt                        44


<210>  56
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (57)..(57)
<223>  T-propanol

<400>  56
ttttccggcg cggcgcgtaa gcgccgcgcc ggtttttttt tttttttttt ttttttt          57


<210>  57
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (6)..(8)
<223>  furan amidite

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  57
tttttnnntt tttttttttt tttttttttt                                        30


<210>  58
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (7)..(9)
<223>  furan amidite

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  58
ttttttnnnt tttttttttt tttttttttt                                        30


<210>  59
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(8)
<223>  furan amidite

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  59
ttttnnnntt tttttttttt tttttttttt                                        30


<210>  60
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(9)
<223>  furan amidite

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  60
ttttnnnnnt tttttttttt tttttttttt                                        30


<210>  61
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  dodecyl amidite-phosphate

<220>
<221>  misc_feature
<222>  (29)..(29)
<223>  T-propanol

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  61
tttttntttt tttttttttt ttttttttt                                         29


<210>  62
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(6)
<223>  hexyl amidite

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  62
ttttnntttt tttttttttt tttttttttt                                        30


<210>  63
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(7)
<223>  propyl amidite

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  63
ttttnnnttt tttttttttt tttttttttt                                        30


<210>  64
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  64
tttttttttt tttttttttt tttttttttt                                        30


<210>  65
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (3)..(10)
<223>  furan amidite

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  65
ttnnnnnnnn tttttttttt tttttttttt                                        30


<210>  66
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (31)..(33)
<223>  propyl amidite

<220>
<221>  misc_feature
<222>  (34)..(34)
<223>  propyl amidite-propanol

<400>  66
tttttttttt tttttttttt tttttttttt nnnn                                   34


<210>  67
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-phosphate

<400>  67
tttttttttt tttttttttt tttttttttt                                        30


<210>  68
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propylamine

<400>  68
tttttttttt tttttttttt tttttttttt                                        30


<210>  69
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (3)..(10)
<223>  furan amidite

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  69
ttnnnnnnnn tttttttttt tttttttttt                                        30


<210>  70
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  phosphate-T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-cyanine 3-phosphate-propylamine-phosphate-proparyl-propionamide

<400>  70
tttttttttt tttttttttt tttttttttt                                        30


<210>  71
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  phosphate-T

<220>
<221>  misc_feature
<222>  (25)..(27)
<223>  furan amidite

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-cyanine 3-phosphate-propylamine-phosphate-proparyl-propionamide

<400>  71
tttttttttt tttttttttt ttttnnnttt                                        30


<210>  72
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (45)..(45)
<223>  T-propanol

<400>  72
ttttcggcgc gtaagcgccg tttttttttt tttttttttt ttttt                       45


<210>  73
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-C

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  C-propanol

<400>  73
cccccccccc cccccccccc cccccccccc                                        30


<210>  74
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(10)
<223>  2'-deoxyinosine

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  74
ttttnnnnnn tttttttttt tttttttttt                                        30


<210>  75
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(10)
<223>  5-nitroindole

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  75
ttttnnnnnn tttttttttt tttttttttt                                        30


<210>  76
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  76
tttcccccct tttttttttt tttttttttt                                        30


<210>  77
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(10)
<223>  5-iodo deoxyuridine

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  77
ttttnnnnnn tttttttttt tttttttttt                                        30


<210>  78
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(10)
<223>  5-pyrene-deoxyuridine

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  78
ttttnnnnnn tttttttttt tttttttttt                                        30


<210>  79
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (3)..(30)
<223>  T-propanol

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  furan amidite

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  furan amidite

<220>
<221>  misc_feature
<222>  (9)..(9)
<223>  furan amidite

<220>
<221>  misc_feature
<222>  (11)..(11)
<223>  furan amidite

<400>  79
ttttntntnt nttttttttt ttttttttt                                         29


<210>  80
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (6)..(6)
<223>  furan amidite

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  furan amidite

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  furan amidite

<220>
<221>  misc_feature
<222>  (12)..(12)
<223>  furan amidite

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  80
tttttntntn tntttttttt ttttttttt                                         29


<210>  81
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(10)
<223>  propanol

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  81
ttttnnnnnn tttttttttt tttttttttt                                        30


<210>  82
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-L isomer of T

<220>
<221>  misc_feature
<222>  (2)..(29)
<223>  L isomer of T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  L isomer of T-propanol

<400>  82
tttttttttt tttttttttt tttttttttt                                        30


<210>  83
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-L isomer of T

<220>
<221>  misc_feature
<222>  (2)..(4)
<223>  L isomer of T

<220>
<221>  misc_feature
<222>  (5)..(7)
<223>  furan amidite

<220>
<221>  misc_feature
<222>  (8)..(29)
<223>  L isomer of T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  L isomer of T-propanol

<400>  83
ttttnnnttt tttttttttt tttttttttt                                        30


<210>  84
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-L isomer of T

<220>
<221>  misc_feature
<222>  (2)..(4)
<223>  L isomer of T

<220>
<221>  misc_feature
<222>  (5)..(12)
<223>  furan amidite

<220>
<221>  misc_feature
<222>  (13)..(29)
<223>  L isomer of T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  L isomer of T-propanol

<400>  84
ttttnnnnnn nntttttttt tttttttttt                                        30


<210>  85
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-L isomer of T

<220>
<221>  misc_feature
<222>  (2)..(4)
<223>  L isomer of T

<220>
<221>  misc_feature
<222>  (5)..(10)
<223>  2'-deoxy I

<220>
<221>  misc_feature
<222>  (11)..(29)
<223>  L isomer of T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  L isomer of T-propanol

<400>  85
ttttnnnnnn tttttttttt tttttttttt                                        30


<210>  86
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (45)..(54)
<223>  T-propanol

<400>  86
ttttgggtgg gtgggtgggt tttttttttt tttttttttt ttttt                       45


<210>  87
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (46)..(46)
<223>  T-propanol

<400>  87
ttttgggtgg gttgggtggg tttttttttt tttttttttt tttttt                      46


<210>  88
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(6)
<223>  dodecyl amidite

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  88
ttttnntttt tttttttttt tttttttttt                                        30


<210>  89
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (4)..(6)
<223>  dodecyl amidite

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  89
tttnnntttt tttttttttt tttttttttt                                        30


<210>  90
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(8)
<223>  hexyl amidite

<220>
<221>  misc_feature
<222>  (33)..(33)
<223>  T-propanol

<400>  90
ttttnnnntt tttttttttt tttttttttt ttt                                    33


<210>  91
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(9)
<223>  hexyl amidite

<220>
<221>  misc_feature
<222>  (32)..(32)
<223>  T-propanol

<400>  91
ttttnnnnnt tttttttttt tttttttttt tt                                     32


<210>  92
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (6)..(9)
<223>  hexyl amidite

<220>
<221>  misc_feature
<222>  (33)..(33)
<223>  T-propanol

<400>  92
tttttnnnnt tttttttttt tttttttttt ttt                                    33


<210>  93
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (3)..(7)
<223>  hexyl amidite

<220>
<221>  misc_feature
<222>  (32)..(32)
<223>  T-propanol

<400>  93
ttnnnnnttt tttttttttt tttttttttt tt                                     32


<210>  94
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  spermine

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  94
ttttnttttt tttttttttt tttttttttt                                        30


<210>  95
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (3)..(3)
<223>  spermine

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  95
ttnttttttt tttttttttt tttttttttt                                        30


<210>  96
<211>  30
<212>  DNA
<213>  Artificial sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (3)..(4)
<223>  spermine

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  96
ttnntttttt tttttttttt tttttttttt                                        30


<210>  97
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(5)
<223>  5-pyrene-deoxyuridine

<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  5-pyrene-deoxyuridine

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  97
ttttnttntt tttttttttt tttttttttt                                        30


<210>  98
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  A-diaminobutryic acid

<400>  98
aaaaaaaaaa aaaaa                                                        15


<210>  99
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthesized peptide

<400>  99

Arg Arg Arg Arg Arg 
1               5   


<210>  100
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  u

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (15)..(15)
<223>  A-diaminobutryic acid

<400>  100
aaaaaaanaa aaaaa                                                        15


<210>  101
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(10)
<223>  thymidine methylphosphonate

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propyl

<400>  101
ttttnnnnnn tttttttttt tttttttttt                                        30


<210>  102
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(10)
<223>  pyrrolidine

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  102
ttttnnnnnt tttttttttt tttttttttt                                        30


<210>  103
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-pyrrolidine-phosphate-T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  103
tttttttttt tttttttttt tttttttttt                                        30


<210>  104
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-pyrrolidine-phosphate-pyrrolidine-phosphate-T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  104
tttttttttt tttttttttt tttttttttt                                        30


<210>  105
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-pyrrolidine-phosphate-pyrrolidine-phosphate-pyrr
       olidine-phosphate-T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  105
tttttttttt tttttttttt tttttttttt                                        30


<210>  106
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-propyl amidite-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  106
tttttttttt tttttttttt tttttttttt                                        30


<210>  107
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-propyl amidite-propyl amidite-cyanine 
       3-phosphate-T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  107
tttttttttt tttttttttt tttttttttt                                        30


<210>  108
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-hexyl amidite-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  108
tttttttttt tttttttttt tttttttttt                                        30


<210>  109
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  hexyne-phosphate-cyanine 3-phosphate-T

<220>
<221>  misc_feature
<222>  (5)..(7)
<223>  alpha anomer of T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-propanol

<400>  109
tttttttttt tttttttttt tttttttttt                                        30


<210>  110
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide

<400>  110
gcgctcgaga tctcctcgta agaggagatc tcgagcgcac tgactgactg acctcagctg       60

cacgtaagtg cagctgaggt cag                                               83


<210>  111
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  biotin-T

<400>  111
tttttttttt tttttttttt tttttttttt                                        30


<210>  112
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  biotin-T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-furan amidite-furan amidite-furan amidite

<400>  112
tttttttttt tttttttttt tttttttttt                                        30


<210>  113
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  biotin-T

<220>
<221>  misc_feature
<222>  (31)..(33)
<223>  any nt

<220>
<221>  misc_feature
<222>  (31)..(33)
<223>  any

<400>  113
tttttttttt tttttttttt tttttttttt nnn                                    33


<210>  114
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  biotin-T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  t-propanol

<400>  114
tttttttttt tttttttttt tttttttttt                                        30


<210>  115
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  biotin-T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-hexanol

<400>  115
tttttttttt tttttttttt tttttttttt                                        30


<210>  116
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  biotin-T

<220>
<221>  misc_feature
<222>  (30)..(30)
<223>  T-furan amidite-propanol

<400>  116
tttttttttt tttttttttt tttttttttt                                        30


<210>  117
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  biotin-T

<220>
<221>  misc_feature
<222>  (10)..(12)
<223>  thymidine methyl phosphonate

<220>
<221>  misc_feature
<222>  (16)..(18)
<223>  thymidine methyl phosphonate

<400>  117
tttttttttn nntttnnntt tttttttttt                                        30


<210>  118
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide

<400>  118
agaggagatc tcgagcgcac tgactgcgtg acctcagctg cacgtaagtg cagctgaggt       60

cac                                                                     63


<210>  119
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide


<220>
<221>  misc_feature
<222>  (47)..(47)
<223>  any nt

<400>  119
gcgctcgaga tctcctcgta agaggagatc tcgagcgcac tgactgnctg acctcagctg       60

cacgtaagtg cagctgaggt cag                                               83


<210>  120
<211>  92
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide

<400>  120
gcacacaagc ttaccttttg gtaagcttgt gtcgaaaatt ttcccctagt agaagcaagt       60

gttttcactt gcttctacta ggggaaaatt tt                                     92


<210>  121
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide

<400>  121
tcagtcagtg cgctcgagat                                                   20


<210>  122
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide

<400>  122
ggggaaaatt tt                                                           12


<210>  123
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide

<400>  123
agtcagtcac gcgagctcta                                                   20


<210>  124
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide

<400>  124
atactgactg actgactgac ctcaga                                            26


<210>  125
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthesized oligonucleotide

<400>  125
gctctaatct gacctcagt                                                    19


