                         SEQUENCE LISTING

<110>  NATIONAL INSTITUTE OF PHARMACEUTICAL EDUCATION AND RESEARCH
 
<120>  RECOMBINANT SSOPOX ENZYMES, METHOD OF GENERATION AND REUSABLE 
       NANOBIOCATALYST OF SAME

<130>  IP26555

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  948
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rSsoPox(wt or wild type)

<400>  1
atgcgtattc cgctggttgg caaagatagc attgaatcta aagacatcgg ttttaccctg       60

attcatgaac acctgcgtgt cttctccgaa gcggtgcgcc agcaatggcc gcatctgtat      120

aacgaagatg aagaatttcg taacgccgtc aatgaagtga aacgcgcaat gcagttcggc      180

gtgaaaacca tcgttgatcc gacggtcatg ggcctgggtc gtgacattcg ctttatggaa      240

aaagtggtta aagctaccgg tatcaacctg gttgcgggca cgggtatcta catctacatc      300

gatctgccgt tctacttcct gaatcgcagc atcgatgaaa ttgccgacct gtttatccac      360

gatattaaag aaggcattca aggtaccctg aataaagcag gcttcgtgaa aatcgcggcc      420

gatgaaccgg gtattacgaa agacgttgaa aaagtcatcc gtgcagctgc gattgctaac      480

aaagaaacca aagttccgat tatcacgcat agcaatgcgc acaacaatac cggcctggaa      540

cagcaacgca ttctgacgga agaaggcgtc gatccgggta aaatcctgat tggccatctg      600

ggtgataccg acaacatcga ttacattaag aaaattgctg acaaaggctc ttttatcggt      660

ctggatcgtt acggtctgga cctgttcctg ccggtggata aacgtaatga aaccacgctg      720

cgcctgatta aagatggcta tagtgacaaa atcatgattt cccacgatta ctgctgtacc      780

atcgactggg gtacggccaa accggaatat aaaccgaaac tggcaccgcg ttggtcaatt      840

accctgatct ttgaagatac gattccgttc ctgaaacgca acggcgtgaa tgaagaagtt      900

attgccacca tctttaaaga aaacccgaaa aaatttttct cgctcgag                   948


<210>  2
<211>  948
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rSsoPox(variant-A)

<400>  2
atgcgtattc cgctggttgg caaagatagc attgaatcta aagacatcgg ttttaccctg       60

attcatgaac acctgcgtgt cttctccgaa gcggtgcgcc agcaatggcc gcatctgaat      120

aacgaagatg aagaatttcg taacgccgtc aatgaagtga aacgcgcaat gcagttcggc      180

gtgaaaacca tcgttgatcc gacggtcatg ggcctgggtc gtgacattcg ctttatggaa      240

aaagtggtta aagctaccgg tatcaacctg gttgcgggca cgggtatcta catctatatc      300

gatctgccgt tctacttcct gaatcgcagc atcgatgaaa ttgccgacct gtttatccac      360

gatattaaag aaggcattca aggtaccctg aataaagcag gcttcgtgaa aatcgcggcc      420

gatgaaccgg gtattacgaa agacgttgaa aaagtcatcc gtgcagctgc gattgctaac      480

aaagaaacca aagttccgat tatcacgcat agcaatgcgc acaacaatac cggcctggaa      540

cagcaacgca ttctgacgga agaaggcgtc gatccgggta aaatcctgat tggccatctg      600

ggtgataccg acaacatcga ttacattaag aaaattgctg acaaaggctc ttttatcggt      660

ctggatcgtt acggtctgga cctgttcctg ccggtggata aacgtaatga aaccacgctg      720

cgcctgatta aagatggcta tagtgacaaa atcatgattt cccacgatta ctgctgtacc      780

atcgactggg gtacggccaa accggaatat aaaccgaaac tggcaccgcg ttggtcaatt      840

accctgatct ttgaagatac gattccgttc ctgaaacgca acggcgtgga tgaagaagtt      900

attgccacca tctttaaaga aaacccgaaa aaatttttct cgctcgag                   948


<210>  3
<211>  316
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rSsoPox(wt or wild type)

<400>  3

Met Arg Ile Pro Leu Val Gly Lys Asp Ser Ile Glu Ser Lys Asp Ile 
1               5                   10                  15      


Gly Phe Thr Leu Ile His Glu His Leu Arg Val Phe Ser Glu Ala Val 
            20                  25                  30          


Arg Gln Gln Trp Pro His Leu Tyr Asn Glu Asp Glu Glu Phe Arg Asn 
        35                  40                  45              


Ala Val Asn Glu Val Lys Arg Ala Met Gln Phe Gly Val Lys Thr Ile 
    50                  55                  60                  


Val Asp Pro Thr Val Met Gly Leu Gly Arg Asp Ile Arg Phe Met Glu 
65                  70                  75                  80  


Lys Val Val Lys Ala Thr Gly Ile Asn Leu Val Ala Gly Thr Gly Ile 
                85                  90                  95      


Tyr Ile Tyr Ile Asp Leu Pro Phe Tyr Phe Leu Asn Arg Ser Ile Asp 
            100                 105                 110         


Glu Ile Ala Asp Leu Phe Ile His Asp Ile Lys Glu Gly Ile Gln Gly 
        115                 120                 125             


Thr Leu Asn Lys Ala Gly Phe Val Lys Ile Ala Ala Asp Glu Pro Gly 
    130                 135                 140                 


Ile Thr Lys Asp Val Glu Lys Val Ile Arg Ala Ala Ala Ile Ala Asn 
145                 150                 155                 160 


Lys Glu Thr Lys Val Pro Ile Ile Thr His Ser Asn Ala His Asn Asn 
                165                 170                 175     


Thr Gly Leu Glu Gln Gln Arg Ile Leu Thr Glu Glu Gly Val Asp Pro 
            180                 185                 190         


Gly Lys Ile Leu Ile Gly His Leu Gly Asp Thr Asp Asn Ile Asp Tyr 
        195                 200                 205             


Ile Lys Lys Ile Ala Asp Lys Gly Ser Phe Ile Gly Leu Asp Arg Tyr 
    210                 215                 220                 


Gly Leu Asp Leu Phe Leu Pro Val Asp Lys Arg Asn Glu Thr Thr Leu 
225                 230                 235                 240 


Arg Leu Ile Lys Asp Gly Tyr Ser Asp Lys Ile Met Ile Ser His Asp 
                245                 250                 255     


Tyr Cys Cys Thr Ile Asp Trp Gly Thr Ala Lys Pro Glu Tyr Lys Pro 
            260                 265                 270         


Lys Leu Ala Pro Arg Trp Ser Ile Thr Leu Ile Phe Glu Asp Thr Ile 
        275                 280                 285             


Pro Phe Leu Lys Arg Asn Gly Val Asn Glu Glu Val Ile Ala Thr Ile 
    290                 295                 300                 


Phe Lys Glu Asn Pro Lys Lys Phe Phe Ser Leu Glu 
305                 310                 315     


<210>  4
<211>  316
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  rSsoPox(variant-A)

<400>  4

Met Arg Ile Pro Leu Val Gly Lys Asp Ser Ile Glu Ser Lys Asp Ile 
1               5                   10                  15      


Gly Phe Thr Leu Ile His Glu His Leu Arg Val Phe Ser Glu Ala Val 
            20                  25                  30          


Arg Gln Gln Trp Pro His Leu Asn Asn Glu Asp Glu Glu Phe Arg Asn 
        35                  40                  45              


Ala Val Asn Glu Val Lys Arg Ala Met Gln Phe Gly Val Lys Thr Ile 
    50                  55                  60                  


Val Asp Pro Thr Val Met Gly Leu Gly Arg Asp Ile Arg Phe Met Glu 
65                  70                  75                  80  


Lys Val Val Lys Ala Thr Gly Ile Asn Leu Val Ala Gly Thr Gly Ile 
                85                  90                  95      


Tyr Ile Tyr Ile Asp Leu Pro Phe Tyr Phe Leu Asn Arg Ser Ile Asp 
            100                 105                 110         


Glu Ile Ala Asp Leu Phe Ile His Asp Ile Lys Glu Gly Ile Gln Gly 
        115                 120                 125             


Thr Leu Asn Lys Ala Gly Phe Val Lys Ile Ala Ala Asp Glu Pro Gly 
    130                 135                 140                 


Ile Thr Lys Asp Val Glu Lys Val Ile Arg Ala Ala Ala Ile Ala Asn 
145                 150                 155                 160 


Lys Glu Thr Lys Val Pro Ile Ile Thr His Ser Asn Ala His Asn Asn 
                165                 170                 175     


Thr Gly Leu Glu Gln Gln Arg Ile Leu Thr Glu Glu Gly Val Asp Pro 
            180                 185                 190         


Gly Lys Ile Leu Ile Gly His Leu Gly Asp Thr Asp Asn Ile Asp Tyr 
        195                 200                 205             


Ile Lys Lys Ile Ala Asp Lys Gly Ser Phe Ile Gly Leu Asp Arg Tyr 
    210                 215                 220                 


Gly Leu Asp Leu Phe Leu Pro Val Asp Lys Arg Asn Glu Thr Thr Leu 
225                 230                 235                 240 


Arg Leu Ile Lys Asp Gly Tyr Ser Asp Lys Ile Met Ile Ser His Asp 
                245                 250                 255     


Tyr Cys Cys Thr Ile Asp Trp Gly Thr Ala Lys Pro Glu Tyr Lys Pro 
            260                 265                 270         


Lys Leu Ala Pro Arg Trp Ser Ile Thr Leu Ile Phe Glu Asp Thr Ile 
        275                 280                 285             


Pro Phe Leu Lys Arg Asn Gly Val Asp Glu Glu Val Ile Ala Thr Ile 
    290                 295                 300                 


Phe Lys Glu Asn Pro Lys Lys Phe Phe Ser Leu Glu 
305                 310                 315     