                         SEQUENCE LISTING

<110>  Dow AgroSciences LLC
 
<120>  Cry1D for Controlling Corn Earworm

<130>  DAS-74266

<150>  61968703
<151>  2014-03-21

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  3420
<212>  DNA
<213>  artificial

<220>
<223>  DIG-911 DNA; Cry1Da2/Cry1Ab chimeric toxin, from pDOW2848

<400>  1
atggaaataa ataatcaaaa ccaatgtgtg ccttacaatt gtttaagtaa tcctaaggag       60

ataatattag gcgaggaaag gctagaaaca gggaatactg tagcagacat ttcattaggg      120

cttattaatt ttctatattc taattttgta ccaggaggag gatttatagt aggtttacta      180

gaattaatat ggggatttat agggccttcg caatgggata tttttttagc tcaaattgag      240

caattgatta gtcaaagaat agaagaattt gctaggaatc aggcaatttc aagattggag      300

gggctaagca atctttataa ggtctatgtt agagcgttta gcgactggga gaaagatcct      360

actaatcctg ctttaaggga agaaatgcgt atacaattta atgacatgaa tagtgctctc      420

ataacggcta ttccactttt tagagttcaa aattatgaag ttgctctttt atctgtatat      480

gttcaagccg caaacttaca tttatctatt ttaagggatg tttcagtttt cggagaaaga      540

tggggatatg atacagcgac tatcaataat cgctatagtg atctgactag ccttattcat      600

gtttatacta accattgtgt ggatacgtat aatcagggat taaggcgttt ggaaggtcgt      660

tttcttagcg attggattgt atataatcgt ttccggagac aattgacaat ttcagtatta      720

gatattgttg cgttttttcc aaattatgat attagaacat atccaattca aacagctact      780

cagctaacga gggaagtcta tctggattta ccttttatta atgaaaatct ttctcctgca      840

gcaagctatc caaccttttc agctgctgaa agtgctataa ttagaagtcc tcatttagta      900

gactttttaa atagctttac catttataca gatagtctgg cacgttatgc atattgggga      960

gggcacttgg taaattcttt ccgcacagga accactacta atttgataag atccccttta     1020

tatggaaggg aaggaaatac agagcgcccc gtaactatta ccgcatcacc tagcgtacca     1080

atatttagaa cactttcata tattacaggc cttgacaatt caaatcctgt agctggaatc     1140

gagggagtgg aattccaaaa tactataagt agaagtatct atcgtaaaag cggtccaata     1200

gattctttta gtgaattacc acctcaagat gccagcgtat ctcctgcaat tgggtatagt     1260

caccgtttat gccatgcaac atttttagaa cggattagtg gaccaagaat agcaggcacc     1320

gtattttctt ggacacaccg tagtgccagc cctactaatg aagtaagtcc atctagaatt     1380

acacaaattc catgggtaaa ggcgcatact cttgcatctg gtgcctccgt cattaaaggt     1440

cctggattta caggtggaga tattctgact aggaatagta tgggcgagct ggggacctta     1500

cgagtaacct tcacaggaag attaccacaa agttattata tacgtttccg ttatgcttcg     1560

gtagcaaata ggagtggtac atttagatat tcacagccac cttcgtatgg aatttcattt     1620

ccaaaaacta tggacgcagg tgaaccacta acatctcgtt cgttcgctca tacaacactc     1680

ttcactccaa taaccttttc acgagctcaa gaagaatttg atctatacat ccaatcgggt     1740

gtttatatag atcgaattga atttataccg gttactgcaa cactggaggc agagtctgac     1800

ttggaaagag cacagaaggc ggtgaatgct ctgttcactt cgtccaatca gattgggctc     1860

aagacagatg tgactgacta tcacatcgat cgcgtttcca accttgttga gtgcctctct     1920

gatgagttct gtttggatga gaagaaggag ttgtccgaga aggtcaaaca tgctaagcga     1980

cttagtgatg agcggaactt gcttcaagat cccaactttc gcgggatcaa caggcaacta     2040

gatcgtggat ggaggggaag tacggacatc accattcaag gaggtgatga tgtgttcaag     2100

gagaactatg ttacgctctt gggtaccttt gatgagtgct atccaacata cctgtaccag     2160

aagatagatg aatcgaaact caaagcctac acaagatacc agttgagagg ttacatcgag     2220

gacagtcaag accttgagat ctacctcatc agatacaacg ccaaacatga gacagtcaat     2280

gtgcctggga cgggttcact ctggccactt tcagccccaa gtcccatcgg caagtgtgcc     2340

catcactcac accacttctc cttggacata gacgttggct gtaccgacct gaacgaagac     2400

ctcggtgtgt gggtgatctt caagatcaag actcaagatg gccatgccag gctaggcaat     2460

ctggagtttc tagaagagaa accacttgtt ggagaagccc tcgctagagt gaagagggct     2520

gagaagaagt ggagggacaa gagagagaag ttggaatggg aaacaaacat tgtgtacaaa     2580

gaagccaaag aaagcgttga cgctctgttt gtgaactctc agtatgatag gctccaagct     2640

gataccaaca tagctatgat tcatgctgca gacaaacgcg ttcatagcat tcgggaagct     2700

taccttcctg aacttagcgt gattccgggt gtcaatgctg ctatctttga agagttagaa     2760

gggcgcatct tcactgcatt ctccttgtat gatgcgagga atgtcatcaa gaatggtgac     2820

ttcaacaatg gcctatcctg ctggaatgtg aaagggcacg tagatgtaga agaacagaac     2880

aatcaccgct ctgtccttgt tgttcctgag tgggaagcag aagtttcaca agaagttcgt     2940

gtctgtcctg gtcgtggcta cattcttcgt gttaccgcgt acaaagaagg atacggagaa     3000

ggttgcgtca ccatacacga gattgagaac aacaccgacg agctgaagtt cagcaactgc     3060

gtcgaggagg aagtctaccc aaacaacacc gtaacttgca atgactacac tgcgactcaa     3120

gaggagtatg agggtactta cacttctcgc aatcgaggat acgatggagc ctatgagagc     3180

aactcttctg tacccgctga ctatgcatca gcctatgagg agaaggctta caccgatgga     3240

cgtagggaca atccttgcga atctaacaga ggctatgggg actacacacc gttaccagcc     3300

ggctatgtca ccaaagagtt agagtacttt ccagaaaccg acaaggtttg gattgagatt     3360

ggagaaacgg aaggaacatt cattgttgat agcgtggagt tacttctgat ggaggaatga     3420


<210>  2
<211>  1139
<212>  PRT
<213>  artificial

<220>
<223>  amino acid sequence for the DIG-911 protein (Cry1Da2/Cry1Ab 
       chimeric insecticidal toxin, which consists of a core toxin 
       segment of Cry1Da (amino acids 1 to 594, as disclosed in GENBANK 
       Accession No. 176415.1 and U.S Patent No. 5,691,308) and a 
       

<400>  2

Met Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser 
1               5                   10                  15      


Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly Asn 
            20                  25                  30          


Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser Asn 
        35                  40                  45              


Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile Trp 
    50                  55                  60                  


Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile Glu 
65                  70                  75                  80  


Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile 
                85                  90                  95      


Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg Ala 
            100                 105                 110         


Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu 
        115                 120                 125             


Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala Ile 
    130                 135                 140                 


Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr 
145                 150                 155                 160 


Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val 
                165                 170                 175     


Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 
            180                 185                 190         


Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp 
        195                 200                 205             


Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser Asp 
    210                 215                 220                 


Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu 
225                 230                 235                 240 


Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile 
                245                 250                 255     


Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro Phe 
            260                 265                 270         


Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe Ser Ala 
        275                 280                 285             


Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 
    290                 295                 300                 


Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Tyr Ala Tyr Trp Gly 
305                 310                 315                 320 


Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu Ile 
                325                 330                 335     


Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val Thr 
            340                 345                 350         


Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Ile 
        355                 360                 365             


Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val Glu 
    370                 375                 380                 


Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro Ile 
385                 390                 395                 400 


Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala 
                405                 410                 415     


Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile 
            420                 425                 430         


Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser 
        435                 440                 445             


Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile Pro 
    450                 455                 460                 


Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly 
465                 470                 475                 480 


Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly Glu 
                485                 490                 495     


Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser Tyr 
            500                 505                 510         


Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr Phe 
        515                 520                 525             


Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met 
    530                 535                 540                 


Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr Leu 
545                 550                 555                 560 


Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu Tyr 
                565                 570                 575     


Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val Thr 
            580                 585                 590         


Ala Thr Leu Glu Ala Glu Ser Asp Leu Glu Arg Ala Gln Lys Ala Val 
        595                 600                 605             


Asn Ala Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val 
    610                 615                 620                 


Thr Asp Tyr His Ile Asp Arg Val Ser Asn Leu Val Glu Cys Leu Ser 
625                 630                 635                 640 


Asp Glu Phe Cys Leu Asp Glu Lys Lys Glu Leu Ser Glu Lys Val Lys 
                645                 650                 655     


His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn 
            660                 665                 670         


Phe Arg Gly Ile Asn Arg Gln Leu Asp Arg Gly Trp Arg Gly Ser Thr 
        675                 680                 685             


Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val 
    690                 695                 700                 


Thr Leu Leu Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln 
705                 710                 715                 720 


Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg 
                725                 730                 735     


Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr 
            740                 745                 750         


Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp 
        755                 760                 765             


Pro Leu Ser Ala Pro Ser Pro Ile Gly Lys Cys Ala His His Ser His 
    770                 775                 780                 


His Phe Ser Leu Asp Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp 
785                 790                 795                 800 


Leu Gly Val Trp Val Ile Phe Lys Ile Lys Thr Gln Asp Gly His Ala 
                805                 810                 815     


Arg Leu Gly Asn Leu Glu Phe Leu Glu Glu Lys Pro Leu Val Gly Glu 
            820                 825                 830         


Ala Leu Ala Arg Val Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg 
        835                 840                 845             


Glu Lys Leu Glu Trp Glu Thr Asn Ile Val Tyr Lys Glu Ala Lys Glu 
    850                 855                 860                 


Ser Val Asp Ala Leu Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala 
865                 870                 875                 880 


Asp Thr Asn Ile Ala Met Ile His Ala Ala Asp Lys Arg Val His Ser 
                885                 890                 895     


Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser Val Ile Pro Gly Val Asn 
            900                 905                 910         


Ala Ala Ile Phe Glu Glu Leu Glu Gly Arg Ile Phe Thr Ala Phe Ser 
        915                 920                 925             


Leu Tyr Asp Ala Arg Asn Val Ile Lys Asn Gly Asp Phe Asn Asn Gly 
    930                 935                 940                 


Leu Ser Cys Trp Asn Val Lys Gly His Val Asp Val Glu Glu Gln Asn 
945                 950                 955                 960 


Asn His Arg Ser Val Leu Val Val Pro Glu Trp Glu Ala Glu Val Ser 
                965                 970                 975     


Gln Glu Val Arg Val Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr 
            980                 985                 990         


Ala Tyr Lys Glu Gly Tyr Gly Glu  Gly Cys Val Thr Ile  His Glu Ile 
        995                 1000                 1005             


Glu Asn  Asn Thr Asp Glu Leu  Lys Phe Ser Asn Cys  Val Glu Glu 
    1010                 1015                 1020             


Glu Val  Tyr Pro Asn Asn Thr  Val Thr Cys Asn Asp  Tyr Thr Ala 
    1025                 1030                 1035             


Thr Gln  Glu Glu Tyr Glu Gly  Thr Tyr Thr Ser Arg  Asn Arg Gly 
    1040                 1045                 1050             


Tyr Asp  Gly Ala Tyr Glu Ser  Asn Ser Ser Val Pro  Ala Asp Tyr 
    1055                 1060                 1065             


Ala Ser  Ala Tyr Glu Glu Lys  Ala Tyr Thr Asp Gly  Arg Arg Asp 
    1070                 1075                 1080             


Asn Pro  Cys Glu Ser Asn Arg  Gly Tyr Gly Asp Tyr  Thr Pro Leu 
    1085                 1090                 1095             


Pro Ala  Gly Tyr Val Thr Lys  Glu Leu Glu Tyr Phe  Pro Glu Thr 
    1100                 1105                 1110             


Asp Lys  Val Trp Ile Glu Ile  Gly Glu Thr Glu Gly  Thr Phe Ile 
    1115                 1120                 1125             


Val Asp  Ser Val Glu Leu Leu  Leu Met Glu Glu 
    1130                 1135                 


<210>  3
<211>  3447
<212>  DNA
<213>  artificial

<220>
<223>  DIG-180 DNA; Cry1Fa2

<400>  3
atggaaaata atattcaaaa tcaatgcgta ccttacaatt gtttaaataa tcctgaagta       60

gaaatactga acgaagaacg cagcaccggc cgcctgccgc tggacatcag cctgagcctt      120

acacgtttcc ttttgagtga atttgttcca ggtgtgggag ttgcgtttgg attatttgat      180

ttaatatggg gttttataac tccttctgat tggagcttat ttcttttaca gattgaacaa      240

ttgattgagc aaagaataga aacattggaa aggaaccggg caattactac attacgaggg      300

ttagcagata gctatgaaat ttatattgaa gcactaagag agtgggaagc aaatcctaat      360

aatgcacaat taagggaaga tgtgcgtatt cgatttgcta atacagacga cgctttaata      420

acagcaataa ataattttac acttacaagt tttgaaatcc ctcttttatc ggtctatgtt      480

caagcggcga atttacattt atcactatta agagacgcag tatcgtttgg gcagggttgg      540

ggactggata tagctactgt taataatcat tataatagat taataaatct tattcataga      600

tatacgaaac attgtttgga cacatacaat caaggattag aaaacttaag aggtactaat      660

actcgacaat gggcaagatt caatcagttt aggagagatt taacacttac tgtattagat      720

atcgttgctc tttttccgaa ctacgatgtt agaacatatc caattcaaac gtcatcccaa      780

ttaacaaggg aaatttatac aagttcagta attgaggatt ctccagtttc tgctaatata      840

cctaatggtt ttaatagggc ggaatttgga gttagaccgc cccatcttat ggactttatg      900

aattctttgt ttgtaactgc agagactgtt agaagtcaaa ctgtgtgggg aggacactta      960

gttagttcac gaaatacggc tggtaaccgt ataaatttcc ctagttacgg ggtcttcaat     1020

cctggtggcg ccatttggat tgcagatgag gatccacgtc ctttttatcg gacattatca     1080

gatcctgttt ttgtccgagg aggatttggg aatcctcatt atgtactggg gcttagggga     1140

gtagcatttc aacaaactgg tacgaaccac acccgaacat ttagaaatag tgggaccata     1200

gattctctag atgaaatccc acctcaggat aatagtgggg caccttggaa tgattatagt     1260

catgtattaa atcatgttac atttgtacga tggccaggtg agatttcagg aagtgattca     1320

tggagagctc caatgttttc ttggacgcac cgtagtgcaa cccctacaaa tacaattgat     1380

ccggagagga ttactcaaat accattggta aaagcacata cacttcagtc aggtactact     1440

gttgtaagag ggcccgggtt tacgggagga gatattcttc gacgaacaag tggaggacca     1500

tttgcttata ctattgttaa tataaatggg caattacccc aaaggtatcg tgcaagaata     1560

cgctatgcct ctactacaaa tctaagaatt tacgtaacgg ttgcaggtga acggattttt     1620

gctggtcaat ttaacaaaac aatggatacc ggtgacccat taacattcca atcttttagt     1680

tacgcaacta ttaatacagc ttttacattc ccaatgagcc agagtagttt cacagtaggt     1740

gctgatactt ttagttcagg gaatgaagtt tatatagaca gatttgaatt gattccagtt     1800

actgcaacat tggaagcaga atctgattta gaaagagcac aaaaggcggt gaatgcgctg     1860

tttacttcta gcaaccaaat agggctaaaa acagatgtga cggattatca tatcgatcga     1920

gtatccaatt tagttgagtg tttatctgat gaattttgtc tggatgaaaa aaaagaattg     1980

tccgagaaag tcaaacatgc gaagcgactt agtgatgagc ggaatttact tcaagatcca     2040

aactttagag ggatcaatag acaactagac cgtggctgga gaggaagtac ggatattacc     2100

atccaaggag gcgatgacgt attcaaagag aattacgtta cgctattggg tacctttgat     2160

gagtgctatc caacgtattt atatcaaaaa atagatgagt cgaaattaaa agcctatacc     2220

cgttaccaat taagagggta tatcgaagat agtcaagact tagaaatcta tttaattcgc     2280

tacaatgcca aacacgaaac agtaaatgtg ccaggtacgg gttccttatg gccgctttca     2340

gccccaagtc caatcggaaa atgtgcccat cattcccatc atttctcctt ggacattgat     2400

gttggatgta cagacttaaa tgaggactta ggtgtatggg tgatattcaa gattaagacg     2460

caagatggcc atgcaagact aggaaatcta gaatttctcg aagagaaacc attagtagga     2520

gaagcactag ctcgtgtgaa aagagcggag aaaaaatgga gagacaaacg tgaaaaattg     2580

gaatgggaaa caaatattgt ttataaagag gcaaaagaat ctgtagatgc tttatttgta     2640

aactctcaat atgatagatt acaagcggat accaacatcg cgatgattca tgcggcagat     2700

aaacgcgttc atagcattcg agaagcttat ctgcctgagc tgtctgtgat tccgggtgtc     2760

aatgcggcta tttttgaaga attagaaggg cgtattttca ctgcattctc cctatatgat     2820

gcgagaaatg tcattaaaaa tggtgatttt aataatggct tatcctgctg gaacgtgaaa     2880

gggcatgtag atgtagaaga acaaaacaac caccgttcgg tccttgttgt tccggaatgg     2940

gaagcagaag tgtcacaaga agttcgtgtc tgtccgggtc gtggctatat ccttcgtgtc     3000

acagcgtaca aggagggata tggagaaggt tgcgtaacca ttcatgagat cgagaacaat     3060

acagacgaac tgaagtttag caactgtgta gaagaggaag tatatccaaa caacacggta     3120

acgtgtaatg attatactgc gactcaagaa gaatatgagg gtacgtacac ttctcgtaat     3180

cgaggatatg acggagccta tgaaagcaat tcttctgtac cagctgatta tgcatcagcc     3240

tatgaagaaa aagcatatac agatggacga agagacaatc cttgtgaatc taacagagga     3300

tatggggatt acacaccact accagctggc tatgtgacaa aagaattaga gtacttccca     3360

gaaaccgata aggtatggat tgagatcgga gaaacggaag gaacattcat cgtggacagc     3420

gtggaattac ttcttatgga ggaataa                                         3447


<210>  4
<211>  1148
<212>  PRT
<213>  artificial

<220>
<223>  DIG-180 protein; Cry1Fa2

<400>  4

Met Glu Asn Asn Ile Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Asn 
1               5                   10                  15      


Asn Pro Glu Val Glu Ile Leu Asn Glu Glu Arg Ser Thr Gly Arg Leu 
            20                  25                  30          


Pro Leu Asp Ile Ser Leu Ser Leu Thr Arg Phe Leu Leu Ser Glu Phe 
        35                  40                  45              


Val Pro Gly Val Gly Val Ala Phe Gly Leu Phe Asp Leu Ile Trp Gly 
    50                  55                  60                  


Phe Ile Thr Pro Ser Asp Trp Ser Leu Phe Leu Leu Gln Ile Glu Gln 
65                  70                  75                  80  


Leu Ile Glu Gln Arg Ile Glu Thr Leu Glu Arg Asn Arg Ala Ile Thr 
                85                  90                  95      


Thr Leu Arg Gly Leu Ala Asp Ser Tyr Glu Ile Tyr Ile Glu Ala Leu 
            100                 105                 110         


Arg Glu Trp Glu Ala Asn Pro Asn Asn Ala Gln Leu Arg Glu Asp Val 
        115                 120                 125             


Arg Ile Arg Phe Ala Asn Thr Asp Asp Ala Leu Ile Thr Ala Ile Asn 
    130                 135                 140                 


Asn Phe Thr Leu Thr Ser Phe Glu Ile Pro Leu Leu Ser Val Tyr Val 
145                 150                 155                 160 


Gln Ala Ala Asn Leu His Leu Ser Leu Leu Arg Asp Ala Val Ser Phe 
                165                 170                 175     


Gly Gln Gly Trp Gly Leu Asp Ile Ala Thr Val Asn Asn His Tyr Asn 
            180                 185                 190         


Arg Leu Ile Asn Leu Ile His Arg Tyr Thr Lys His Cys Leu Asp Thr 
        195                 200                 205             


Tyr Asn Gln Gly Leu Glu Asn Leu Arg Gly Thr Asn Thr Arg Gln Trp 
    210                 215                 220                 


Ala Arg Phe Asn Gln Phe Arg Arg Asp Leu Thr Leu Thr Val Leu Asp 
225                 230                 235                 240 


Ile Val Ala Leu Phe Pro Asn Tyr Asp Val Arg Thr Tyr Pro Ile Gln 
                245                 250                 255     


Thr Ser Ser Gln Leu Thr Arg Glu Ile Tyr Thr Ser Ser Val Ile Glu 
            260                 265                 270         


Asp Ser Pro Val Ser Ala Asn Ile Pro Asn Gly Phe Asn Arg Ala Glu 
        275                 280                 285             


Phe Gly Val Arg Pro Pro His Leu Met Asp Phe Met Asn Ser Leu Phe 
    290                 295                 300                 


Val Thr Ala Glu Thr Val Arg Ser Gln Thr Val Trp Gly Gly His Leu 
305                 310                 315                 320 


Val Ser Ser Arg Asn Thr Ala Gly Asn Arg Ile Asn Phe Pro Ser Tyr 
                325                 330                 335     


Gly Val Phe Asn Pro Gly Gly Ala Ile Trp Ile Ala Asp Glu Asp Pro 
            340                 345                 350         


Arg Pro Phe Tyr Arg Thr Leu Ser Asp Pro Val Phe Val Arg Gly Gly 
        355                 360                 365             


Phe Gly Asn Pro His Tyr Val Leu Gly Leu Arg Gly Val Ala Phe Gln 
    370                 375                 380                 


Gln Thr Gly Thr Asn His Thr Arg Thr Phe Arg Asn Ser Gly Thr Ile 
385                 390                 395                 400 


Asp Ser Leu Asp Glu Ile Pro Pro Gln Asp Asn Ser Gly Ala Pro Trp 
                405                 410                 415     


Asn Asp Tyr Ser His Val Leu Asn His Val Thr Phe Val Arg Trp Pro 
            420                 425                 430         


Gly Glu Ile Ser Gly Ser Asp Ser Trp Arg Ala Pro Met Phe Ser Trp 
        435                 440                 445             


Thr His Arg Ser Ala Thr Pro Thr Asn Thr Ile Asp Pro Glu Arg Ile 
    450                 455                 460                 


Thr Gln Ile Pro Leu Val Lys Ala His Thr Leu Gln Ser Gly Thr Thr 
465                 470                 475                 480 


Val Val Arg Gly Pro Gly Phe Thr Gly Gly Asp Ile Leu Arg Arg Thr 
                485                 490                 495     


Ser Gly Gly Pro Phe Ala Tyr Thr Ile Val Asn Ile Asn Gly Gln Leu 
            500                 505                 510         


Pro Gln Arg Tyr Arg Ala Arg Ile Arg Tyr Ala Ser Thr Thr Asn Leu 
        515                 520                 525             


Arg Ile Tyr Val Thr Val Ala Gly Glu Arg Ile Phe Ala Gly Gln Phe 
    530                 535                 540                 


Asn Lys Thr Met Asp Thr Gly Asp Pro Leu Thr Phe Gln Ser Phe Ser 
545                 550                 555                 560 


Tyr Ala Thr Ile Asn Thr Ala Phe Thr Phe Pro Met Ser Gln Ser Ser 
                565                 570                 575     


Phe Thr Val Gly Ala Asp Thr Phe Ser Ser Gly Asn Glu Val Tyr Ile 
            580                 585                 590         


Asp Arg Phe Glu Leu Ile Pro Val Thr Ala Thr Leu Glu Ala Glu Ser 
        595                 600                 605             


Asp Leu Glu Arg Ala Gln Lys Ala Val Asn Ala Leu Phe Thr Ser Ser 
    610                 615                 620                 


Asn Gln Ile Gly Leu Lys Thr Asp Val Thr Asp Tyr His Ile Asp Arg 
625                 630                 635                 640 


Val Ser Asn Leu Val Glu Cys Leu Ser Asp Glu Phe Cys Leu Asp Glu 
                645                 650                 655     


Lys Lys Glu Leu Ser Glu Lys Val Lys His Ala Lys Arg Leu Ser Asp 
            660                 665                 670         


Glu Arg Asn Leu Leu Gln Asp Pro Asn Phe Arg Gly Ile Asn Arg Gln 
        675                 680                 685             


Leu Asp Arg Gly Trp Arg Gly Ser Thr Asp Ile Thr Ile Gln Gly Gly 
    690                 695                 700                 


Asp Asp Val Phe Lys Glu Asn Tyr Val Thr Leu Leu Gly Thr Phe Asp 
705                 710                 715                 720 


Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln Lys Ile Asp Glu Ser Lys Leu 
                725                 730                 735     


Lys Ala Tyr Thr Arg Tyr Gln Leu Arg Gly Tyr Ile Glu Asp Ser Gln 
            740                 745                 750         


Asp Leu Glu Ile Tyr Leu Ile Arg Tyr Asn Ala Lys His Glu Thr Val 
        755                 760                 765             


Asn Val Pro Gly Thr Gly Ser Leu Trp Pro Leu Ser Ala Pro Ser Pro 
    770                 775                 780                 


Ile Gly Lys Cys Ala His His Ser His His Phe Ser Leu Asp Ile Asp 
785                 790                 795                 800 


Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val Ile Phe 
                805                 810                 815     


Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu Glu Phe 
            820                 825                 830         


Leu Glu Glu Lys Pro Leu Val Gly Glu Ala Leu Ala Arg Val Lys Arg 
        835                 840                 845             


Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Lys Leu Glu Trp Glu Thr 
    850                 855                 860                 


Asn Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu Phe Val 
865                 870                 875                 880 


Asn Ser Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn Ile Ala Met Ile 
                885                 890                 895     


His Ala Ala Asp Lys Arg Val His Ser Ile Arg Glu Ala Tyr Leu Pro 
            900                 905                 910         


Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu Glu Leu 
        915                 920                 925             


Glu Gly Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg Asn Val 
    930                 935                 940                 


Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Ser Cys Trp Asn Val Lys 
945                 950                 955                 960 


Gly His Val Asp Val Glu Glu Gln Asn Asn His Arg Ser Val Leu Val 
                965                 970                 975     


Val Pro Glu Trp Glu Ala Glu Val Ser Gln Glu Val Arg Val Cys Pro 
            980                 985                 990         


Gly Arg Gly Tyr Ile Leu Arg Val  Thr Ala Tyr Lys Glu  Gly Tyr Gly 
        995                 1000                 1005             


Glu Gly  Cys Val Thr Ile His  Glu Ile Glu Asn Asn  Thr Asp Glu 
    1010                 1015                 1020             


Leu Lys  Phe Ser Asn Cys Val  Glu Glu Glu Val Tyr  Pro Asn Asn 
    1025                 1030                 1035             


Thr Val  Thr Cys Asn Asp Tyr  Thr Ala Thr Gln Glu  Glu Tyr Glu 
    1040                 1045                 1050             


Gly Thr  Tyr Thr Ser Arg Asn  Arg Gly Tyr Asp Gly  Ala Tyr Glu 
    1055                 1060                 1065             


Ser Asn  Ser Ser Val Pro Ala  Asp Tyr Ala Ser Ala  Tyr Glu Glu 
    1070                 1075                 1080             


Lys Ala  Tyr Thr Asp Gly Arg  Arg Asp Asn Pro Cys  Glu Ser Asn 
    1085                 1090                 1095             


Arg Gly  Tyr Gly Asp Tyr Thr  Pro Leu Pro Ala Gly  Tyr Val Thr 
    1100                 1105                 1110             


Lys Glu  Leu Glu Tyr Phe Pro  Glu Thr Asp Lys Val  Trp Ile Glu 
    1115                 1120                 1125             


Ile Gly  Glu Thr Glu Gly Thr  Phe Ile Val Asp Ser  Val Glu Leu 
    1130                 1135                 1140             


Leu Leu  Met Glu Glu 
    1145             


<210>  5
<211>  1812
<212>  DNA
<213>  artificial

<220>
<223>  Cry1Da2 v4 DNA from pDAB109841 (truncated Cry1Da; maize 
       optimized)

<400>  5
atggagatca acaaccagaa tcagtgcgtt ccttacaact gtctgagcaa ccccaaagaa       60

atcatccttg gcgaggagag gctggaaact ggcaacacag tggctgacat tagcttgggt      120

cttatcaact tcttgtactc aaactttgtt cccggtggag gcttcattgt gggactgctt      180

gagctgatat ggggtttcat aggtccgagc cagtgggaca tctttctggc acaaatcgag      240

cagctcatct cacagcggat tgaggagttt gcgagaaatc aagccatatc gagactcgaa      300

gggctgtcca acttgtacaa ggtctatgtg agggctttct ctgattggga gaaggacccc      360

acgaatccag cgcttcgcga ggagatgagg atacagttca atgacatgaa ctctgcgttg      420

atcacggcta ttccgctctt tagggtgcag aactacgagg ttgctctgct ctcagtgtac      480

gtgcaagctg ccaacttgca tctgagcatc ctccgggacg tctcggtgtt tggggaacgg      540

tggggttatg acaccgcaac gatcaacaac cgctattcag accttacatc tcttatccac      600

gtgtacacga atcactgcgt tgatacgtac aatcaaggcc tccgcagact cgaagggagg      660

ttcctcagcg attggattgt ttacaatcgc ttcagacggc aactcacaat ctcggttctg      720

gacatagtcg cgttcttccc gaactatgat atccgcacct atcccattca gaccgctact      780

cagctcactc gcgaagtgta tcttgacctc ccgttcatca atgagaactt gtcaccagca      840

gcgtcctatc ccaccttctc agctgcggag tccgctatca tccgctcccc acatctggtt      900

gatttcctca actctttcac tatctacacc gactcgcttg cgagatacgc atactggggt      960

ggccatctgg tgaactcatt ccggactggc accacgacca atctgatccg cagccctctc     1020

tacggacgcg agggcaacac cgagaggcca gtgaccatca ccgcttcccc ttccgttcct     1080

atcttccgca ccctttcgta cattactggc ctcgacaaca gcaacccagt cgctggcatc     1140

gagggtgttg agtttcagaa caccatttct aggtctatct ataggaagag cggtccaata     1200

gactcgtttt ctgagttgcc tccccaagat gcctctgtca gcccagccat tggctactcc     1260

catcggctct gtcacgccac cttccttgaa cgcatctccg gaccaaggat cgctgggacg     1320

gtctttagct ggacccaccg ctcagcatct ccgacaaatg aggtctcccc ttcccgcatc     1380

acacaaatcc cgtgggtgaa ggcacacaca ttggcctcgg gagcctcggt catcaaaggg     1440

cctggcttca ctggaggcga cattctgacg aggaactcaa tgggtgagct ggggaccttg     1500

agggtcactt tcactggacg cctcccacag tcctactaca ttcggttccg ctatgccagc     1560

gtggccaata ggtccggaac attccgctac agccagccac ccagctacgg cattagcttc     1620

cctaagacta tggatgctgg ggaacctctg acctcaaggt cgtttgccca cacgacgctg     1680

ttcaccccta tcacattcag cagagcacaa gaggagtttg atctgtacat ccagtccgga     1740

gtctacattg accggatcga gttcattccg gttactgcga cactcgaggc tgaatcggat     1800

cttgaaaggt ga                                                         1812


<210>  6
<211>  603
<212>  PRT
<213>  artificial

<220>
<223>  Cry1Da2 v4 protein (truncated Cry1Da)

<400>  6

Met Glu Ile Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Ser 
1               5                   10                  15      


Asn Pro Lys Glu Ile Ile Leu Gly Glu Glu Arg Leu Glu Thr Gly Asn 
            20                  25                  30          


Thr Val Ala Asp Ile Ser Leu Gly Leu Ile Asn Phe Leu Tyr Ser Asn 
        35                  40                  45              


Phe Val Pro Gly Gly Gly Phe Ile Val Gly Leu Leu Glu Leu Ile Trp 
    50                  55                  60                  


Gly Phe Ile Gly Pro Ser Gln Trp Asp Ile Phe Leu Ala Gln Ile Glu 
65                  70                  75                  80  


Gln Leu Ile Ser Gln Arg Ile Glu Glu Phe Ala Arg Asn Gln Ala Ile 
                85                  90                  95      


Ser Arg Leu Glu Gly Leu Ser Asn Leu Tyr Lys Val Tyr Val Arg Ala 
            100                 105                 110         


Phe Ser Asp Trp Glu Lys Asp Pro Thr Asn Pro Ala Leu Arg Glu Glu 
        115                 120                 125             


Met Arg Ile Gln Phe Asn Asp Met Asn Ser Ala Leu Ile Thr Ala Ile 
    130                 135                 140                 


Pro Leu Phe Arg Val Gln Asn Tyr Glu Val Ala Leu Leu Ser Val Tyr 
145                 150                 155                 160 


Val Gln Ala Ala Asn Leu His Leu Ser Ile Leu Arg Asp Val Ser Val 
                165                 170                 175     


Phe Gly Glu Arg Trp Gly Tyr Asp Thr Ala Thr Ile Asn Asn Arg Tyr 
            180                 185                 190         


Ser Asp Leu Thr Ser Leu Ile His Val Tyr Thr Asn His Cys Val Asp 
        195                 200                 205             


Thr Tyr Asn Gln Gly Leu Arg Arg Leu Glu Gly Arg Phe Leu Ser Asp 
    210                 215                 220                 


Trp Ile Val Tyr Asn Arg Phe Arg Arg Gln Leu Thr Ile Ser Val Leu 
225                 230                 235                 240 


Asp Ile Val Ala Phe Phe Pro Asn Tyr Asp Ile Arg Thr Tyr Pro Ile 
                245                 250                 255     


Gln Thr Ala Thr Gln Leu Thr Arg Glu Val Tyr Leu Asp Leu Pro Phe 
            260                 265                 270         


Ile Asn Glu Asn Leu Ser Pro Ala Ala Ser Tyr Pro Thr Phe Ser Ala 
        275                 280                 285             


Ala Glu Ser Ala Ile Ile Arg Ser Pro His Leu Val Asp Phe Leu Asn 
    290                 295                 300                 


Ser Phe Thr Ile Tyr Thr Asp Ser Leu Ala Arg Tyr Ala Tyr Trp Gly 
305                 310                 315                 320 


Gly His Leu Val Asn Ser Phe Arg Thr Gly Thr Thr Thr Asn Leu Ile 
                325                 330                 335     


Arg Ser Pro Leu Tyr Gly Arg Glu Gly Asn Thr Glu Arg Pro Val Thr 
            340                 345                 350         


Ile Thr Ala Ser Pro Ser Val Pro Ile Phe Arg Thr Leu Ser Tyr Ile 
        355                 360                 365             


Thr Gly Leu Asp Asn Ser Asn Pro Val Ala Gly Ile Glu Gly Val Glu 
    370                 375                 380                 


Phe Gln Asn Thr Ile Ser Arg Ser Ile Tyr Arg Lys Ser Gly Pro Ile 
385                 390                 395                 400 


Asp Ser Phe Ser Glu Leu Pro Pro Gln Asp Ala Ser Val Ser Pro Ala 
                405                 410                 415     


Ile Gly Tyr Ser His Arg Leu Cys His Ala Thr Phe Leu Glu Arg Ile 
            420                 425                 430         


Ser Gly Pro Arg Ile Ala Gly Thr Val Phe Ser Trp Thr His Arg Ser 
        435                 440                 445             


Ala Ser Pro Thr Asn Glu Val Ser Pro Ser Arg Ile Thr Gln Ile Pro 
    450                 455                 460                 


Trp Val Lys Ala His Thr Leu Ala Ser Gly Ala Ser Val Ile Lys Gly 
465                 470                 475                 480 


Pro Gly Phe Thr Gly Gly Asp Ile Leu Thr Arg Asn Ser Met Gly Glu 
                485                 490                 495     


Leu Gly Thr Leu Arg Val Thr Phe Thr Gly Arg Leu Pro Gln Ser Tyr 
            500                 505                 510         


Tyr Ile Arg Phe Arg Tyr Ala Ser Val Ala Asn Arg Ser Gly Thr Phe 
        515                 520                 525             


Arg Tyr Ser Gln Pro Pro Ser Tyr Gly Ile Ser Phe Pro Lys Thr Met 
    530                 535                 540                 


Asp Ala Gly Glu Pro Leu Thr Ser Arg Ser Phe Ala His Thr Thr Leu 
545                 550                 555                 560 


Phe Thr Pro Ile Thr Phe Ser Arg Ala Gln Glu Glu Phe Asp Leu Tyr 
                565                 570                 575     


Ile Gln Ser Gly Val Tyr Ile Asp Arg Ile Glu Phe Ile Pro Val Thr 
            580                 585                 590         


Ala Thr Leu Glu Ala Glu Ser Asp Leu Glu Arg 
        595                 600             


<210>  7
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  AAD1F Primer

<400>  7
tgttcggttc cctctaccaa                                                   20


<210>  8
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  AAD1R Primer

<400>  8
caacatccat caccttgact ga                                                22


<210>  9
<211>  24
<212>  DNA
<213>  artificial

<220>
<223>  AAD1P Probe

<400>  9
cacagaaccg tcgcttcagc aaca                                              24


<210>  10
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  SPC1A Primer

<400>  10
cttagctgga taacgccac                                                    19


<210>  11
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  SPC1S Primer

<400>  11
gaccgtaagg cttgatgaa                                                    19


<210>  12
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  TQSPC Probe

<400>  12
cgagattctc cgcgctgtag a                                                 21


<210>  13
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  InvertaseF Primer

<400>  13
tggcggacga cgacttgt                                                     18


<210>  14
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  InvertaseR Primer

<400>  14
aaagtttgga ggctgccgt                                                    19


<210>  15
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  InvertaseP Probe

<400>  15
cgagcagacc gccgtgtact t                                                 21


