                         SEQUENCE LISTING

<110>  The Trustees of the University of Pennsylvania
 
<120>  Compositions and Methods Using Modified Salmonella

<130>  UPN-14-7023PCT

<140>  PCT/US15/_______
<141>  2015-03-17

<150>  61/954269
<151>  2014-03-17

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  2598
<212>  DNA
<213>  Salmonella enterica

<400>  1
gtgctagaag aataccgtaa gcacgtagct gagcgtgctg cccaggggat tgtgccaaaa       60

cctttagacg caacccaaat ggctgcgctt gtcgagctgc tgaagacccc gcctgtgggc      120

gaagaagaat tcctgttaga cctgttgatc aaccgcgttc ctcctggcgt agatgaagcc      180

gcttatgtta aagccggttt tctcgctgct gtcgcgaaag gcgacaccac ctccccgctg      240

gtctccccag aaaaagccat tgaactgctg ggcaccatgc agggtggtta caacattcat      300

ccgctgattg atgcgctgga cgatgcgaaa ctggcgccga ttgctgccaa agcactgtcc      360

cacaccctgc tgatgttcga taacttctac gacgtagaag agaaagccaa agcgggcaat      420

gaatatgcca aacaggtgat gcaatcctgg gccgacgccg aatggttcct gagccgtccg      480

ccgctggcgg aaaaaatcac cgtcaccgtt ttcaaagtga ccggcgaaac gaataccgac      540

gatctctctc cggcgccgga tgcgtggtcg agaccggata tcccgttaca tgcgcaggcg      600

atgctgaaaa acgcccgtga aggcattgag ccggatcagc caggcgttgt cggcccgatc      660

aaacaaatcg aagcattgca gaaaaaaggc tacccgctgg cttacgtggg tgacgtggtg      720

ggcaccggtt cttcccgtaa atccgcgacc aactccgtgc tgtggttcat gggcgatgac      780

atcccgaacg tgccgaataa gcgcggcggc ggtctgtgcc tcggcggcaa aattgcgcct      840

atcttcttta acactatgga agatgcgggc gcgctgccga ttgaagttga cgtttctaac      900

ctgaatatgg gcgatgtgat tgacgtctac ccgtacaaag gcgaagtacg caatcatgaa      960

accggcgagc tactggcaac cttcgaactg aaaaccgacg tgctgattga cgaagtgcgt     1020

gccggtggtc gtattccgct gattatcggc cgtggcctga ccaccaaagc gcgtgaagcg     1080

ctggggctgc cgcactcaga cgttttccgt caggcaaaag acgtggcgga aagcagccgt     1140

ggcttctcgc tggcacagaa aatggtcggt cgcgcctgtg gtgtgaaagg tatccgtccg     1200

ggcgcgtact gcgaaccgaa aatgacctcc gtcggttctc aggatactac cggcccgatg     1260

acccgcgacg agctgaaaga cctggcctgt ctgggattct ccgccgatct ggtcatgcag     1320

tcgttctgtc acaccgcagc ctatccgaag ccggttgacg tcaccacgca ccacacgctg     1380

ccagacttca ttatgaaccg tggcggtgtc tctctgcgtc cgggcgacgg cgtgattcac     1440

tcctggctga accgtatgct gctgccggat accgtcggta ccggcggtga ctcccatacc     1500

cgtttcccga ttggtatctc tttcccggcg ggttctggtc tggtggcgtt tgccgccgcg     1560

accggcgtga tgccgctgga tatgccggaa tccgtgctgg tgcgcttcaa aggcaaaatg     1620

cagccgggca tcaccctgcg cgatctggta cacgccatcc cgctgtacgc catcaaacag     1680

ggcctgctga ccgttgagaa gaaaggcaag aaaaacatct tctctggccg catcctggaa     1740

atcgaaggtc tgccggatct gaaagtcgag caggcgtttg agctgaccga tgcttccgcc     1800

gagcgttccg ctgccggttg taccatcaag ctgaacaaag agccgattgt tgaatacctg     1860

acctccaaca tcgtcctgct gaagtggatg atcgccgaag gctacggcga ccgccgtacg     1920

ctggaacgtc gtatccaggg catggaaaaa tggctggcgg acccgcagct gctggaagcc     1980

gatgctgacg cagagtacgc ggcggtgatc gacatcgatc tggcggatat caaagagcca     2040

atcctctgtg cgccgaacga tccggacgac gcgcgtctgc tgtctgacgt gcagggcgag     2100

aagatcgacg aagtgttcat cggctcctgc atgactaaca tcggccactt ccgtgcagcc     2160

ggcaagctgc tggacaacca taaaggccaa ctgccgactc gcctgtgggt ggcgccgcca     2220

acccgtatgg acgccgcgca gctgaccgaa gaaggttact acagcgtgtt cggtaagagc     2280

ggtgcgcgta ttgagatccc tggctgttcc ctgtgtatgg gtaaccaggc gcgtgtggct     2340

gacggtgcga cggtggtttc cacttctacc cgtaacttcc cgaaccgttt aggtaccggt     2400

gcgaacgtct tcctggcttc tgcggagctg gcggcggttg cggcgctgat cggcaaactg     2460

ccgacgccgg aagagtacca gacctacgtg gcacaggtgg ataagacggc ggtggatact     2520

taccgttacc tgaactttga ccagctttct cagtacaccg agaaagcaga tggcgtgatt     2580

ttccagactg cggtttaa                                                   2598


<210>  2
<211>  865
<212>  PRT
<213>  salmonella enterica

<400>  2

Met Leu Glu Glu Tyr Arg Lys His Val Ala Glu Arg Ala Ala Gln Gly 
1               5                   10                  15      


Ile Val Pro Lys Pro Leu Asp Ala Thr Gln Met Ala Ala Leu Val Glu 
            20                  25                  30          


Leu Leu Lys Thr Pro Pro Val Gly Glu Glu Glu Phe Leu Leu Asp Leu 
        35                  40                  45              


Leu Ile Asn Arg Val Pro Pro Gly Val Asp Glu Ala Ala Tyr Val Lys 
    50                  55                  60                  


Ala Gly Phe Leu Ala Ala Val Ala Lys Gly Asp Thr Thr Ser Pro Leu 
65                  70                  75                  80  


Val Ser Pro Glu Lys Ala Ile Glu Leu Leu Gly Thr Met Gln Gly Gly 
                85                  90                  95      


Tyr Asn Ile His Pro Leu Ile Asp Ala Leu Asp Asp Ala Lys Leu Ala 
            100                 105                 110         


Pro Ile Ala Ala Lys Ala Leu Ser His Thr Leu Leu Met Phe Asp Asn 
        115                 120                 125             


Phe Tyr Asp Val Glu Glu Lys Ala Lys Ala Gly Asn Glu Tyr Ala Lys 
    130                 135                 140                 


Gln Val Met Gln Ser Trp Ala Asp Ala Glu Trp Phe Leu Ser Arg Pro 
145                 150                 155                 160 


Pro Leu Ala Glu Lys Ile Thr Val Thr Val Phe Lys Val Thr Gly Glu 
                165                 170                 175     


Thr Asn Thr Asp Asp Leu Ser Pro Ala Pro Asp Ala Trp Ser Arg Pro 
            180                 185                 190         


Asp Ile Pro Leu His Ala Gln Ala Met Leu Lys Asn Ala Arg Glu Gly 
        195                 200                 205             


Ile Glu Pro Asp Gln Pro Gly Val Val Gly Pro Ile Lys Gln Ile Glu 
    210                 215                 220                 


Ala Leu Gln Lys Lys Gly Tyr Pro Leu Ala Tyr Val Gly Asp Val Val 
225                 230                 235                 240 


Gly Thr Gly Ser Ser Arg Lys Ser Ala Thr Asn Ser Val Leu Trp Phe 
                245                 250                 255     


Met Gly Asp Asp Ile Pro Asn Val Pro Asn Lys Arg Gly Gly Gly Leu 
            260                 265                 270         


Cys Leu Gly Gly Lys Ile Ala Pro Ile Phe Phe Asn Thr Met Glu Asp 
        275                 280                 285             


Ala Gly Ala Leu Pro Ile Glu Val Asp Val Ser Asn Leu Asn Met Gly 
    290                 295                 300                 


Asp Val Ile Asp Val Tyr Pro Tyr Lys Gly Glu Val Arg Asn His Glu 
305                 310                 315                 320 


Thr Gly Glu Leu Leu Ala Thr Phe Glu Leu Lys Thr Asp Val Leu Ile 
                325                 330                 335     


Asp Glu Val Arg Ala Gly Gly Arg Ile Pro Leu Ile Ile Gly Arg Gly 
            340                 345                 350         


Leu Thr Thr Lys Ala Arg Glu Ala Leu Gly Leu Pro His Ser Asp Val 
        355                 360                 365             


Phe Arg Gln Ala Lys Asp Val Ala Glu Ser Ser Arg Gly Phe Ser Leu 
    370                 375                 380                 


Ala Gln Lys Met Val Gly Arg Ala Cys Gly Val Lys Gly Ile Arg Pro 
385                 390                 395                 400 


Gly Ala Tyr Cys Glu Pro Lys Met Thr Ser Val Gly Ser Gln Asp Thr 
                405                 410                 415     


Thr Gly Pro Met Thr Arg Asp Glu Leu Lys Asp Leu Ala Cys Leu Gly 
            420                 425                 430         


Phe Ser Ala Asp Leu Val Met Gln Ser Phe Cys His Thr Ala Ala Tyr 
        435                 440                 445             


Pro Lys Pro Val Asp Val Thr Thr His His Thr Leu Pro Asp Phe Ile 
    450                 455                 460                 


Met Asn Arg Gly Gly Val Ser Leu Arg Pro Gly Asp Gly Val Ile His 
465                 470                 475                 480 


Ser Trp Leu Asn Arg Met Leu Leu Pro Asp Thr Val Gly Thr Gly Gly 
                485                 490                 495     


Asp Ser His Thr Arg Phe Pro Ile Gly Ile Ser Phe Pro Ala Gly Ser 
            500                 505                 510         


Gly Leu Val Ala Phe Ala Ala Ala Thr Gly Val Met Pro Leu Asp Met 
        515                 520                 525             


Pro Glu Ser Val Leu Val Arg Phe Lys Gly Lys Met Gln Pro Gly Ile 
    530                 535                 540                 


Thr Leu Arg Asp Leu Val His Ala Ile Pro Leu Tyr Ala Ile Lys Gln 
545                 550                 555                 560 


Gly Leu Leu Thr Val Glu Lys Lys Gly Lys Lys Asn Ile Phe Ser Gly 
                565                 570                 575     


Arg Ile Leu Glu Ile Glu Gly Leu Pro Asp Leu Lys Val Glu Gln Ala 
            580                 585                 590         


Phe Glu Leu Thr Asp Ala Ser Ala Glu Arg Ser Ala Ala Gly Cys Thr 
        595                 600                 605             


Ile Lys Leu Asn Lys Glu Pro Ile Val Glu Tyr Leu Thr Ser Asn Ile 
    610                 615                 620                 


Val Leu Leu Lys Trp Met Ile Ala Glu Gly Tyr Gly Asp Arg Arg Thr 
625                 630                 635                 640 


Leu Glu Arg Arg Ile Gln Gly Met Glu Lys Trp Leu Ala Asp Pro Gln 
                645                 650                 655     


Leu Leu Glu Ala Asp Ala Asp Ala Glu Tyr Ala Ala Val Ile Asp Ile 
            660                 665                 670         


Asp Leu Ala Asp Ile Lys Glu Pro Ile Leu Cys Ala Pro Asn Asp Pro 
        675                 680                 685             


Asp Asp Ala Arg Leu Leu Ser Asp Val Gln Gly Glu Lys Ile Asp Glu 
    690                 695                 700                 


Val Phe Ile Gly Ser Cys Met Thr Asn Ile Gly His Phe Arg Ala Ala 
705                 710                 715                 720 


Gly Lys Leu Leu Asp Asn His Lys Gly Gln Leu Pro Thr Arg Leu Trp 
                725                 730                 735     


Val Ala Pro Pro Thr Arg Met Asp Ala Ala Gln Leu Thr Glu Glu Gly 
            740                 745                 750         


Tyr Tyr Ser Val Phe Gly Lys Ser Gly Ala Arg Ile Glu Ile Pro Gly 
        755                 760                 765             


Cys Ser Leu Cys Met Gly Asn Gln Ala Arg Val Ala Asp Gly Ala Thr 
    770                 775                 780                 


Val Val Ser Thr Ser Thr Arg Asn Phe Pro Asn Arg Leu Gly Thr Gly 
785                 790                 795                 800 


Ala Asn Val Phe Leu Ala Ser Ala Glu Leu Ala Ala Val Ala Ala Leu 
                805                 810                 815     


Ile Gly Lys Leu Pro Thr Pro Glu Glu Tyr Gln Thr Tyr Val Ala Gln 
            820                 825                 830         


Val Asp Lys Thr Ala Val Asp Thr Tyr Arg Tyr Leu Asn Phe Asp Gln 
        835                 840                 845             


Leu Ser Gln Tyr Thr Glu Lys Ala Asp Gly Val Ile Phe Gln Thr Ala 
    850                 855                 860                 


Val 
865 


<210>  3
<211>  1251
<212>  DNA
<213>  Salmonella enterica

<400>  3
atggaaagca aagtagttgt tccggtggaa ggtaagaaga tcaccctgca aaacggcaaa       60

ctcaacgttc ctgagaatcc gattatcccg tttattgaag gcgatggtat cggtgttgat      120

gtcactccag ccatgcttaa agtagtcgat gccgccgtcg agaaagccta taaaggcgag      180

cgtaaaattt cctggatgga aatttacacc ggagaaaaat ccacacaggt ttacggccag      240

gatgtctggc ttcccgctga aacccttgat ttaattcgtg actaccgcgt agcgattaaa      300

ggtccattga ccacgccggt tggcggcggt atccgctctc tgaacgttgc cctgcgtcag      360

gagcttgacc tgtacgtttg tctgcgtccc gttcgttact atcagggcac gccaagtccg      420

gtgaaacatc cggagctgac cgatatggtc atcttccgtg aaaactctga agacatctac      480

gccggaattg aatggaaagc tgactctgca gacgctgaga aagtgatcaa gttcctgcgc      540

gaagagatgg gcgtgaagaa aattcgcttc ccggagcact gcggtatcgg tatcaaaccg      600

tgttctgaag aaggcactaa acgtctggtt cgcgcggcga ttgaatacgc tattaccaac      660

gatcgcgatt ccgtcacgct ggtacataaa ggcaatatca tgaagttcac cgaaggcgcg      720

tttaaagact ggggctacca gttggcgcgc gacgagttcg gcggtgagct tattgacggc      780

ggtccgtggc tgaaaattaa gaacccgaac acgggtaaag agatcgtggt taaagacgtt      840

atcgccgatg cgttcctgca acaaatcctg ttgcgtccgg cggaatacga cgttatcgcc      900

tgtatgaacc tgaacggtga ctatatttcc gatgccctgg cggctcaggt tggcggtatc      960

ggtattgcac cgggtgcgaa tatcggcgac gaatgcgcgt tgtttgaagc gactcacggg     1020

actgcgccga aatatgcggg ccaggataaa gtcaacccag gctccattat tctgtctgcg     1080

gagatgatgc tgcgccacat gcagtggttc gaagccgcag acctgattgt taaaggtatg     1140

gaaggcgcga ttgccgcgaa gaccgtgacc tatgactttg aacgcctgat ggaaggcgct     1200

aagctgctga aatgtagtga gtttggtgac gcgattatcg cgaatatgta a              1251


<210>  4
<211>  416
<212>  PRT
<213>  Salmonella enterica

<400>  4

Met Glu Ser Lys Val Val Val Pro Val Glu Gly Lys Lys Ile Thr Leu 
1               5                   10                  15      


Gln Asn Gly Lys Leu Asn Val Pro Glu Asn Pro Ile Ile Pro Phe Ile 
            20                  25                  30          


Glu Gly Asp Gly Ile Gly Val Asp Val Thr Pro Ala Met Leu Lys Val 
        35                  40                  45              


Val Asp Ala Ala Val Glu Lys Ala Tyr Lys Gly Glu Arg Lys Ile Ser 
    50                  55                  60                  


Trp Met Glu Ile Tyr Thr Gly Glu Lys Ser Thr Gln Val Tyr Gly Gln 
65                  70                  75                  80  


Asp Val Trp Leu Pro Ala Glu Thr Leu Asp Leu Ile Arg Asp Tyr Arg 
                85                  90                  95      


Val Ala Ile Lys Gly Pro Leu Thr Thr Pro Val Gly Gly Gly Ile Arg 
            100                 105                 110         


Ser Leu Asn Val Ala Leu Arg Gln Glu Leu Asp Leu Tyr Val Cys Leu 
        115                 120                 125             


Arg Pro Val Arg Tyr Tyr Gln Gly Thr Pro Ser Pro Val Lys His Pro 
    130                 135                 140                 


Glu Leu Thr Asp Met Val Ile Phe Arg Glu Asn Ser Glu Asp Ile Tyr 
145                 150                 155                 160 


Ala Gly Ile Glu Trp Lys Ala Asp Ser Ala Asp Ala Glu Lys Val Ile 
                165                 170                 175     


Lys Phe Leu Arg Glu Glu Met Gly Val Lys Lys Ile Arg Phe Pro Glu 
            180                 185                 190         


His Cys Gly Ile Gly Ile Lys Pro Cys Ser Glu Glu Gly Thr Lys Arg 
        195                 200                 205             


Leu Val Arg Ala Ala Ile Glu Tyr Ala Ile Thr Asn Asp Arg Asp Ser 
    210                 215                 220                 


Val Thr Leu Val His Lys Gly Asn Ile Met Lys Phe Thr Glu Gly Ala 
225                 230                 235                 240 


Phe Lys Asp Trp Gly Tyr Gln Leu Ala Arg Asp Glu Phe Gly Gly Glu 
                245                 250                 255     


Leu Ile Asp Gly Gly Pro Trp Leu Lys Ile Lys Asn Pro Asn Thr Gly 
            260                 265                 270         


Lys Glu Ile Val Val Lys Asp Val Ile Ala Asp Ala Phe Leu Gln Gln 
        275                 280                 285             


Ile Leu Leu Arg Pro Ala Glu Tyr Asp Val Ile Ala Cys Met Asn Leu 
    290                 295                 300                 


Asn Gly Asp Tyr Ile Ser Asp Ala Leu Ala Ala Gln Val Gly Gly Ile 
305                 310                 315                 320 


Gly Ile Ala Pro Gly Ala Asn Ile Gly Asp Glu Cys Ala Leu Phe Glu 
                325                 330                 335     


Ala Thr His Gly Thr Ala Pro Lys Tyr Ala Gly Gln Asp Lys Val Asn 
            340                 345                 350         


Pro Gly Ser Ile Ile Leu Ser Ala Glu Met Met Leu Arg His Met Gln 
        355                 360                 365             


Trp Phe Glu Ala Ala Asp Leu Ile Val Lys Gly Met Glu Gly Ala Ile 
    370                 375                 380                 


Ala Ala Lys Thr Val Thr Tyr Asp Phe Glu Arg Leu Met Glu Gly Ala 
385                 390                 395                 400 


Lys Leu Leu Lys Cys Ser Glu Phe Gly Asp Ala Ile Ile Ala Asn Met 
                405                 410                 415     


<210>  5
<211>  24
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TetA primer sequence

<400>  5
acctttggtc accaacgctt ttcc                                              24


<210>  6
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Random sequence primer


<220>
<221>  misc_feature
<222>  (18)..(26)
<223>  n is A or G or T or C

<400>  6
gtttcccagt cacgatcnnn nnnnnn                                            26


<210>  7
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Universal primer

<400>  7
gacaagatgt gtatccacct tacc                                              24


<210>  8
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Random primer

<400>  8
gtttcccagt cacgatc                                                      17


