                         SEQUENCE LISTING

<110>  Le, Quy
       Maizels, Nancy
 
<120>  RESTRICTING NUCLEAR PROTEIN TO SPECIFIC PHASES OF THE CELL CYCLE

<130>  UW.54WOU2

<160>  19    

<170>  PatentIn version 3.5

<210>  1
<211>  270
<212>  DNA
<213>  artificial sequence

<220>
<223>  construct encoding rearranged IgVH region for AID-mCherry


<220>
<221>  CDS
<222>  (1)..(270)

<400>  1
acc tgc ggt gtt tat ggt ggg tcc ttc agt ggt tac tac tgg agc tgg         48
Thr Cys Gly Val Tyr Gly Gly Ser Phe Ser Gly Tyr Tyr Trp Ser Trp           
1               5                   10                  15                

atc cgc cag ccc cca ggg aag ggg ctg gag tgg att ggg gaa atc aat         96
Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Ile Gly Glu Ile Asn           
            20                  25                  30                    

cat agt gga agc acc aac tac aac ccg tcc ctc aag agt cga gtc acc        144
His Ser Gly Ser Thr Asn Tyr Asn Pro Ser Leu Lys Ser Arg Val Thr           
        35                  40                  45                        

ata tca gta gac acg tcc aag aag cag ctc tcc ctg aag ttg agc tct        192
Ile Ser Val Asp Thr Ser Lys Lys Gln Leu Ser Leu Lys Leu Ser Ser           
    50                  55                  60                            

gtg aac gcc gcg gac acg gct gtg tat tac tgt gcg aga gtt att act        240
Val Asn Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala Arg Val Ile Thr           
65                  70                  75                  80            

agg gcg agt cct ggc aca gac ggg agg tac                                270
Arg Ala Ser Pro Gly Thr Asp Gly Arg Tyr                                   
                85                  90                                    


<210>  2
<211>  90
<212>  PRT
<213>  artificial sequence

<220>
<223>  Synthetic Construct

<400>  2

Thr Cys Gly Val Tyr Gly Gly Ser Phe Ser Gly Tyr Tyr Trp Ser Trp 
1               5                   10                  15      


Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Ile Gly Glu Ile Asn 
            20                  25                  30          


His Ser Gly Ser Thr Asn Tyr Asn Pro Ser Leu Lys Ser Arg Val Thr 
        35                  40                  45              


Ile Ser Val Asp Thr Ser Lys Lys Gln Leu Ser Leu Lys Leu Ser Ser 
    50                  55                  60                  


Val Asn Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala Arg Val Ile Thr 
65                  70                  75                  80  


Arg Ala Ser Pro Gly Thr Asp Gly Arg Tyr 
                85                  90  


<210>  3
<211>  270
<212>  DNA
<213>  artificial sequence

<220>
<223>  construct encoding rearranged IgVH region for AID-mCherry-CDT1


<220>
<221>  CDS
<222>  (1)..(270)

<400>  3
acc tgc ggt gtt tat ggt ggg tcc ttc agt ggt tac tac tgg agc tgg         48
Thr Cys Gly Val Tyr Gly Gly Ser Phe Ser Gly Tyr Tyr Trp Ser Trp           
1               5                   10                  15                

atc cgc cag ccc cca ggg aag ggg ctg gag tgg att ggg gaa atc aat         96
Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Ile Gly Glu Ile Asn           
            20                  25                  30                    

cat agt gga agc acc aac tac aac ccg tcc ctc aag agt cga gtc acc        144
His Ser Gly Ser Thr Asn Tyr Asn Pro Ser Leu Lys Ser Arg Val Thr           
        35                  40                  45                        

ata tca gta gac acg tcc aag aag cag ctc tcc ctg aag ttg agc tct        192
Ile Ser Val Asp Thr Ser Lys Lys Gln Leu Ser Leu Lys Leu Ser Ser           
    50                  55                  60                            

gtg aac gcc gcg gac acg gct gtg tat tac tgt gcg aga gtt att act        240
Val Asn Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala Arg Val Ile Thr           
65                  70                  75                  80            

agg gcg agt cct ggc aca gac ggg agg tac                                270
Arg Ala Ser Pro Gly Thr Asp Gly Arg Tyr                                   
                85                  90                                    


<210>  4
<211>  90
<212>  PRT
<213>  artificial sequence

<220>
<223>  Synthetic Construct

<400>  4

Thr Cys Gly Val Tyr Gly Gly Ser Phe Ser Gly Tyr Tyr Trp Ser Trp 
1               5                   10                  15      


Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Ile Gly Glu Ile Asn 
            20                  25                  30          


His Ser Gly Ser Thr Asn Tyr Asn Pro Ser Leu Lys Ser Arg Val Thr 
        35                  40                  45              


Ile Ser Val Asp Thr Ser Lys Lys Gln Leu Ser Leu Lys Leu Ser Ser 
    50                  55                  60                  


Val Asn Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala Arg Val Ile Thr 
65                  70                  75                  80  


Arg Ala Ser Pro Gly Thr Asp Gly Arg Tyr 
                85                  90  


<210>  5
<211>  270
<212>  DNA
<213>  artificial sequence

<220>
<223>  construct encoding rearranged IgVH region for AID-mCherry-GEM


<220>
<221>  CDS
<222>  (1)..(270)

<400>  5
acc tgc ggt gtt tat ggt ggg tcc ttc agt ggt tac tac tgg agc tgg         48
Thr Cys Gly Val Tyr Gly Gly Ser Phe Ser Gly Tyr Tyr Trp Ser Trp           
1               5                   10                  15                

atc cgc cag ccc cca ggg aag ggg ctg gag tgg att ggg gaa atc aat         96
Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Ile Gly Glu Ile Asn           
            20                  25                  30                    

cat agt gga agc acc aac tac aac ccg tcc ctc aag agt cga gtc acc        144
His Ser Gly Ser Thr Asn Tyr Asn Pro Ser Leu Lys Ser Arg Val Thr           
        35                  40                  45                        

ata tca gta gac acg tcc aag aag cag ctc tcc ctg aag ttg agc tct        192
Ile Ser Val Asp Thr Ser Lys Lys Gln Leu Ser Leu Lys Leu Ser Ser           
    50                  55                  60                            

gtg aac gcc gcg gac acg gct gtg tat tac tgt gcg aga gtt att act        240
Val Asn Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala Arg Val Ile Thr           
65                  70                  75                  80            

agg gcg agt cct ggc aca gac ggg agg tac                                270
Arg Ala Ser Pro Gly Thr Asp Gly Arg Tyr                                   
                85                  90                                    


<210>  6
<211>  90
<212>  PRT
<213>  artificial sequence

<220>
<223>  Synthetic Construct

<400>  6

Thr Cys Gly Val Tyr Gly Gly Ser Phe Ser Gly Tyr Tyr Trp Ser Trp 
1               5                   10                  15      


Ile Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Ile Gly Glu Ile Asn 
            20                  25                  30          


His Ser Gly Ser Thr Asn Tyr Asn Pro Ser Leu Lys Ser Arg Val Thr 
        35                  40                  45              


Ile Ser Val Asp Thr Ser Lys Lys Gln Leu Ser Leu Lys Leu Ser Ser 
    50                  55                  60                  


Val Asn Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala Arg Val Ile Thr 
65                  70                  75                  80  


Arg Ala Ser Pro Gly Thr Asp Gly Arg Tyr 
                85                  90  


<210>  7
<211>  29
<212>  DNA
<213>  artificial sequence

<220>
<223>  PQL31 primer

<400>  7
atatcaattg agatcccaaa tggacagcc                                         29


<210>  8
<211>  33
<212>  DNA
<213>  artificial sequence

<220>
<223>  PQL32 primer

<400>  8
atattctaga ttacttgtac agctcgtcca tgc                                    33


<210>  9
<211>  35
<212>  DNA
<213>  artificial sequence

<220>
<223>  PQL44 primer

<400>  9
tatatgtaca agggatatcc atcacactgg cggcc                                  35


<210>  10
<211>  35
<212>  DNA
<213>  artificial sequence

<220>
<223>  PQL45 primer

<400>  10
tatatgtaca tctagattag atggtgtcct ggtcc                                  35


<210>  11
<211>  34
<212>  DNA
<213>  artificial sequence

<220>
<223>  PQL46 primer

<400>  11
tatatgtaca tctagattac agcgcctttc tccg                                   34


<210>  12
<211>  31
<212>  DNA
<213>  artificial sequence

<220>
<223>  mKO2 FOR primer

<400>  12
atatggatcc atcgccacca tggtgagtgt g                                      31


<210>  13
<211>  31
<212>  DNA
<213>  artificial sequence

<220>
<223>  mKO2 REV primer

<400>  13
atatgcggcc gccagtgtga tggatatccg c                                      31


<210>  14
<211>  29
<212>  DNA
<213>  artificial sequence

<220>
<223>  F193A FOR primer

<400>  14
cttacgagac gcagctcgta ctttgggac                                         29


<210>  15
<211>  29
<212>  DNA
<213>  artificial sequence

<220>
<223>  F193A REV primer

<400>  15
gtcccaaagt acgagctgcg tctcgtaag                                         29


<210>  16
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  RVHFOR QL primer

<400>  16
tcccaggtgc agctacagca g                                                 21


<210>  17
<211>  21
<212>  DNA
<213>  artificial sequence

<220>
<223>  JOL48 QL primer

<400>  17
gtacctgagg agacggtgac c                                                 21


<210>  18
<211>  19
<212>  DNA
<213>  artificial sequence

<220>
<223>  2nd round FOR

<400>  18
aggtgcagct acagcagtg                                                    19


<210>  19
<211>  18
<212>  DNA
<213>  artificial sequence

<220>
<223>  2nd round REV primer

<400>  19
gccccagacg tccatacc                                                     18


