                               SEQUENCE LISTING

<110> NOVOGY, INC.
 
<120> REDUCING HORIZONTAL GENE TRANSFER OF FUNCTIONAL PROTEINS

<130> NGX-026.25 (28975-02625)

<140> PCT/US2015/018410
<141> 2015-03-03

<150> 61/947,051
<151> 2014-03-03

<160> 15    

<170> PatentIn version 3.5

<210> 1
<211> 66
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide


<220>
<221> MOD_RES
<222> (2)..(5)
<223> Any amino acid and this region may encompass 2-4 residues

<220>
<221> MOD_RES
<222> (7)..(25)
<223> Any amino acid and this region may encompass 13-19 residues 

<220>
<221> MOD_RES
<222> (28)..(31)
<223> Any amino acid and this region may encompass 2-4 residues 

<220>
<221> MOD_RES
<222> (34)..(34)
<223> Leu, Val or Ile

<220>
<221> MOD_RES
<222> (36)..(39)
<223> Any amino acid and this region may encompass 2-4 residues 

<220>
<221> MOD_RES
<222> (41)..(60)
<223> Any amino acid and this region may encompass 13-20 residues 

<220>
<221> MOD_RES
<222> (62)..(65)
<223> Any amino acid and this region may encompass 2-4 residues 

<400> 1
Cys Xaa Xaa Xaa Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10                  15      


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Trp His Xaa Xaa Xaa Xaa Cys 
            20                  25                  30          


Phe Xaa Cys Xaa Xaa Xaa Xaa Cys Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        35                  40                  45              


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Cys Xaa Xaa Xaa 
    50                  55                  60                  


Xaa Cys 
65      


<210> 2
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (3)..(3)
<223> Any amino acid

<400> 2
Trp Ser Xaa Trp Ser 
1               5   


<210> 3
<211> 19
<212> PRT
<213> Pseudomonas stutzeri

<400> 3
Arg Leu Ile Asp Pro Ala Leu Leu Ala His Pro Asn Thr Leu Phe Thr 
1               5                   10                  15      


Pro His Ile 
            


<210> 4
<211> 10
<212> PRT
<213> Pseudomonas stutzeri

<400> 4
Ser Gly Glu Phe Gln Gly Trp Gln Pro Gln 
1               5                   10  


<210> 5
<211> 25
<212> PRT
<213> Pseudomonas stutzeri

<400> 5
Val Phe Glu Met Glu Asp Trp Ala Arg Ala Asp Arg Pro Arg Leu Ile 
1               5                   10                  15      


Asp Pro Ala Leu Leu Ala His Pro Asn 
            20                  25  


<210> 6
<211> 336
<212> PRT
<213> Pseudomonas stutzeri

<400> 6
Met Leu Pro Lys Leu Val Ile Thr His Arg Val His Asp Glu Ile Leu 
1               5                   10                  15      


Gln Leu Leu Ala Pro His Cys Glu Leu Met Thr Asn Gln Thr Asp Ser 
            20                  25                  30          


Thr Leu Thr Arg Glu Glu Ile Leu Arg Arg Cys Arg Asp Ala Gln Ala 
        35                  40                  45              


Met Met Ala Phe Met Pro Asp Arg Val Asp Ala Asp Phe Leu Gln Ala 
    50                  55                  60                  


Cys Pro Glu Leu Arg Val Val Gly Cys Ala Leu Lys Gly Phe Asp Asn 
65                  70                  75                  80  


Phe Asp Val Asp Ala Cys Thr Ala Arg Gly Val Trp Leu Thr Phe Val 
                85                  90                  95      


Pro Asp Leu Leu Thr Val Pro Thr Ala Glu Leu Ala Ile Gly Leu Ala 
            100                 105                 110         


Val Gly Leu Gly Arg His Leu Arg Ala Ala Asp Ala Phe Val Arg Ser 
        115                 120                 125             


Gly Glu Phe Gln Gly Trp Gln Pro Gln Phe Tyr Gly Thr Gly Leu Asp 
    130                 135                 140                 


Asn Ala Thr Val Gly Ile Leu Gly Met Gly Ala Ile Gly Leu Ala Met 
145                 150                 155                 160 


Ala Asp Arg Leu Gln Gly Trp Gly Ala Thr Leu Gln Tyr His Glu Ala 
                165                 170                 175     


Lys Ala Leu Asp Thr Gln Thr Glu Gln Arg Leu Gly Leu Arg Gln Val 
            180                 185                 190         


Ala Cys Ser Glu Leu Phe Ala Ser Ser Asp Phe Ile Leu Leu Ala Leu 
        195                 200                 205             


Pro Leu Asn Ala Asp Thr Gln His Leu Val Asn Ala Glu Leu Leu Ala 
    210                 215                 220                 


Leu Val Arg Pro Gly Ala Leu Leu Val Asn Pro Cys Arg Gly Ser Val 
225                 230                 235                 240 


Val Asp Glu Ala Ala Val Leu Ala Ala Leu Glu Arg Gly Gln Leu Gly 
                245                 250                 255     


Gly Tyr Ala Ala Asp Val Phe Glu Met Glu Asp Trp Ala Arg Ala Asp 
            260                 265                 270         


Arg Pro Arg Leu Ile Asp Pro Ala Leu Leu Ala His Pro Asn Thr Leu 
        275                 280                 285             


Phe Thr Pro His Ile Gly Ser Ala Val Arg Ala Val Arg Leu Glu Ile 
    290                 295                 300                 


Glu Arg Cys Ala Ala Gln Asn Ile Ile Gln Val Leu Ala Gly Ala Arg 
305                 310                 315                 320 


Pro Ile Asn Ala Ala Asn Arg Leu Pro Lys Ala Glu Pro Ala Ala Cys 
                325                 330                 335     


<210> 7
<211> 1011
<212> DNA
<213> Pseudomonas stutzeri

<400> 7
atgctgccga aactcgttat aactcaccga gtacacgatg agatcctgca actgctggcg       60

ccacattgcg agctgatgac caaccagacc gacagcacgc tgacgcgcga ggaaattctg      120

cgccgctgtc gcgatgctca ggcgatgatg gcgttcatgc ccgatcgggt cgatgcagac      180

tttcttcaag cctgccctga gctgcgtgta gtcggctgcg cgctcaaggg cttcgacaat      240

ttcgatgtgg acgcctgtac tgcccgcggg gtctggctga ccttcgtgcc tgatctgttg      300

acggtcccga ctgccgagct ggcgatcgga ctggcggtgg ggctggggcg gcatctgcgg      360

gcagcagatg cgttcgtccg ctctggcgag ttccagggct ggcaaccaca gttctacggc      420

acggggctgg ataacgctac ggtcggcatc cttggcatgg gcgccatcgg actggccatg      480

gctgatcgct tgcagggatg gggcgcgacc ctgcagtacc acgaggcgaa ggctctggat      540

acacaaaccg agcaacggct cggcctgcgc caggtggcgt gcagcgaact cttcgccagc      600

tcggacttca tcctgctggc gcttcccttg aatgccgata cccagcatct ggtcaacgcc      660

gagctgcttg ccctcgtacg gccgggcgct ctgcttgtaa acccctgtcg tggttcggta      720

gtggatgaag ccgccgtgct cgcggcgctt gagcgaggcc agctcggcgg gtatgcggcg      780

gatgtattcg aaatggaaga ctgggctcgc gcggaccggc cgcggctgat cgatcctgcg      840

ctgctcgcgc atccgaatac gctgttcact ccgcacatag ggtcggcagt gcgcgcggtg      900

cgcctggaga ttgaacgttg tgcagcgcag aacatcatcc aggtattggc aggtgcgcgc      960

ccaatcaacg ctgcgaaccg tctgcccaag gccgagcctg ccgcatgttg a              1011


<210> 8
<211> 281
<212> PRT
<213> Pseudomonas stutzeri

<400> 8
Met Leu Pro Lys Leu Val Ile Thr His Arg Val His Asp Glu Ile Leu 
1               5                   10                  15      


Gln Leu Leu Ala Pro His Cys Glu Leu Met Thr Asn Gln Thr Asp Ser 
            20                  25                  30          


Thr Leu Thr Arg Glu Glu Ile Leu Arg Arg Cys Arg Asp Ala Gln Ala 
        35                  40                  45              


Met Met Ala Phe Met Pro Asp Arg Val Asp Ala Asp Phe Leu Gln Ala 
    50                  55                  60                  


Cys Pro Glu Leu Arg Val Val Gly Cys Ala Leu Lys Gly Phe Asp Asn 
65                  70                  75                  80  


Phe Asp Val Asp Ala Cys Thr Ala Arg Gly Val Trp Leu Thr Phe Val 
                85                  90                  95      


Pro Asp Leu Leu Thr Val Pro Thr Ala Glu Leu Ala Ile Gly Leu Ala 
            100                 105                 110         


Val Gly Leu Gly Arg His Leu Arg Ala Ala Asp Ala Phe Val Arg Ser 
        115                 120                 125             


Gly Glu Phe Gln Gly Trp Gln Pro Gln Phe Tyr Gly Thr Gly Leu Asp 
    130                 135                 140                 


Asn Ala Thr Val Gly Ile Leu Gly Met Gly Ala Ile Gly Leu Ala Met 
145                 150                 155                 160 


Ala Asp Arg Leu Gln Gly Trp Gly Ala Thr Leu Gln Tyr His Glu Ala 
                165                 170                 175     


Lys Ala Leu Asp Thr Gln Thr Glu Gln Arg Leu Gly Leu Arg Gln Val 
            180                 185                 190         


Ala Cys Ser Glu Leu Phe Ala Ser Ser Asp Phe Ile Leu Leu Ala Leu 
        195                 200                 205             


Pro Leu Asn Ala Asp Thr Gln His Leu Val Asn Ala Glu Leu Leu Ala 
    210                 215                 220                 


Leu Val Arg Pro Gly Ala Leu Leu Val Asn Pro Cys Arg Gly Ser Val 
225                 230                 235                 240 


Val Asp Glu Ala Ala Val Leu Ala Ala Leu Glu Arg Gly Gln Leu Gly 
                245                 250                 255     


Gly Tyr Ala Ala Asp Val Phe Glu Met Glu Asp Trp Ala Arg Ala Asp 
            260                 265                 270         


Arg Pro Arg Leu Ile Asp Pro Ala Leu 
        275                 280     


<210> 9
<211> 843
<212> DNA
<213> Pseudomonas stutzeri

<400> 9
atgctgccga aactcgttat aactcaccga gtacacgatg agatcctgca actgctggcg       60

ccacattgcg agctgatgac caaccagacc gacagcacgc tgacgcgcga ggaaattctg      120

cgccgctgtc gcgatgctca ggcgatgatg gcgttcatgc ccgatcgggt cgatgcagac      180

tttcttcaag cctgccctga gctgcgtgta gtcggctgcg cgctcaaggg cttcgacaat      240

ttcgatgtgg acgcctgtac tgcccgcggg gtctggctga ccttcgtgcc tgatctgttg      300

acggtcccga ctgccgagct ggcgatcgga ctggcggtgg ggctggggcg gcatctgcgg      360

gcagcagatg cgttcgtccg ctctggcgag ttccagggct ggcaaccaca gttctacggc      420

acggggctgg ataacgctac ggtcggcatc cttggcatgg gcgccatcgg actggccatg      480

gctgatcgct tgcagggatg gggcgcgacc ctgcagtacc acgaggcgaa ggctctggat      540

acacaaaccg agcaacggct cggcctgcgc caggtggcgt gcagcgaact cttcgccagc      600

tcggacttca tcctgctggc gcttcccttg aatgccgata cccagcatct ggtcaacgcc      660

gagctgcttg ccctcgtacg gccgggcgct ctgcttgtaa acccctgtcg tggttcggta      720

gtggatgaag ccgccgtgct cgcggcgctt gagcgaggcc agctcggcgg gtatgcggcg      780

gatgtattcg aaatggaaga ctgggctcgc gcggaccggc cgcggctgat cgatcctgcg      840

ctg                                                                    843


<210> 10
<211> 55
<212> PRT
<213> Pseudomonas stutzeri

<400> 10
Leu Ala His Pro Asn Thr Leu Phe Thr Pro His Ile Gly Ser Ala Val 
1               5                   10                  15      


Arg Ala Val Arg Leu Glu Ile Glu Arg Cys Ala Ala Gln Asn Ile Ile 
            20                  25                  30          


Gln Val Leu Ala Gly Ala Arg Pro Ile Asn Ala Ala Asn Arg Leu Pro 
        35                  40                  45              


Lys Ala Glu Pro Ala Ala Cys 
    50                  55  


<210> 11
<211> 168
<212> DNA
<213> Pseudomonas stutzeri

<400> 11
ctcgcgcatc cgaatacgct gttcactccg cacatagggt cggcagtgcg cgcggtgcgc       60

ctggagattg aacgttgtgc agcgcagaac atcatccagg tattggcagg tgcgcgccca      120

atcaacgctg cgaaccgtct gcccaaggcc gagcctgccg catgttga                   168


<210> 12
<211> 29
<212> PRT
<213> Pseudomonas stutzeri

<400> 12
Ala Leu Lys Lys Glu Leu Gln Ala Asn Lys Lys Glu Leu Ala Gln Leu 
1               5                   10                  15      


Lys Trp Glu Leu Gln Ala Leu Lys Lys Glu Leu Ala Gln 
            20                  25                  


<210> 13
<211> 90
<212> DNA
<213> Pseudomonas stutzeri

<400> 13
gctttaaaaa aagaattaca agctaataaa aaagaattag ctcaattaaa atgggaatta       60

caagctttaa aaaaagaatt agctcaatga                                        90


<210> 14
<211> 31
<212> PRT
<213> Pseudomonas stutzeri

<400> 14
Met Glu Gln Leu Glu Lys Lys Leu Gln Ala Leu Glu Lys Lys Leu Ala 
1               5                   10                  15      


Gln Leu Glu Trp Lys Asn Gln Ala Leu Glu Lys Lys Leu Ala Gln 
            20                  25                  30      


<210> 15
<211> 93
<212> DNA
<213> Pseudomonas stutzeri

<400> 15
atggaacaat tagaaaaaaa attacaagct ttagaaaaaa aattagctca attagaatgg       60

aaaaatcaag ctttagaaaa aaaattagct caa                                    93


