                         SEQUENCE LISTING

<110>  Scarab Genomics, LLC
 
<120>  Enhanced Production of Recombinant CRM197 in E. Coli

<130>  010447-5037 WO

<140>  PCT/US2015/18338
<141>  2015-03-02

<150>  US 61/947,234
<151>  2014-03-03

<160>  38    

<170>  PatentIn version 3.5

<210>  1
<211>  1608
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRM197 sequence optimized for periplasmic expression

<400>  1
ggtgcggatg atgttgtgga cagctctaag tcttttgtga tggaaaactt tagctcgtac       60

cacggtacga agccaggtta tgtcgacagc attcaaaaag gtatccagaa accgaagtcc      120

ggcacgcagg gtaactacga cgacgattgg aaagagttct acagcaccga caacaagtat      180

gacgcagcgg gttacagcgt tgacaatgag aatccgttga gcggcaaagc gggtggtgtt      240

gtcaaagtga cgtatccggg tctgaccaag gtcctggcgt tgaaagttga taacgcggaa      300

accattaaga aagagctggg tctgagcctg accgagccgt tgatggagca agttggtacc      360

gaagagttta tcaaacgttt cggcgatggt gcgagccgcg ttgtcctgtc cctgcctttc      420

gcggagggca gctccagcgt tgagtatatc aataactggg agcaagcaaa agcgctgtcc      480

gtcgaactgg aaatcaattt tgaaacgcgc ggtaaacgtg gtcaagatgc aatgtacgag      540

tatatggccc aggcctgcgc tggtaatcgt gttcgtcgca gcgttggtag cagcttgtct      600

tgtatcaacc tggattggga tgtgatccgt gataagacca agactaagat cgagagcctg      660

aaagaacatg gcccgattaa gaacaagatg tcggagagcc cgaataagac cgtgagcgaa      720

gaaaaggcca agcagtatct ggaagagttc caccaaacgg ctctggagca tccggagctg      780

agcgagctga aaacggttac gggcaccaac ccggtgttcg caggtgcgaa ttacgcggcg      840

tgggcagtga atgtggcgca ggtcatcgac tccgaaacgg cggacaattt ggagaaaacc      900

accgcagcgc tgagcattct gccgggcatc ggcagcgtta tgggcattgc agatggtgct      960

gtgcaccata acactgaaga aatcgtagcg caaagcattg ccctgtctag cttgatggtg     1020

gcgcaggcta ttccgctggt cggcgaactg gttgatatcg gctttgctgc ctacaacttc     1080

gttgaaagca tcattaacct gtttcaggtg gtccacaaca gctataatcg cccagcgtac     1140

agcccgggtc acaagaccca accgttcctg cacgatggct atgcggtgtc ttggaacacg     1200

gtcgaagata gcatcattcg taccggtttc cagggcgaga gcggccatga catcaagatt     1260

actgcagaaa ataccccgct gccgatcgca ggtgtcctgc tgcctacgat tccgggtaag     1320

ctggacgtta acaaaagcaa aacccacatt tctgtgaacg gtcgtaagat tcgcatgcgt     1380

tgtcgtgcga ttgacggcga cgtcaccttc tgccgtccga agagcccggt ctacgttggt     1440

aatggtgtgc acgcgaacct gcacgtggcg tttcaccgca gcagctcgga gaaaatccat     1500

agcaatgaga tttctagcga cagcattggc gttctgggtt accaaaagac ggttgaccat     1560

accaaagtca attccaaact gagcctgttc tttgagatca aaagctaa                  1608


<210>  2
<211>  535
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Expressed and processed CRM197 amino acid sequence

<400>  2

Gly Ala Asp Asp Val Val Asp Ser Ser Lys Ser Phe Val Met Glu Asn 
1               5                   10                  15      


Phe Ser Ser Tyr His Gly Thr Lys Pro Gly Tyr Val Asp Ser Ile Gln 
            20                  25                  30          


Lys Gly Ile Gln Lys Pro Lys Ser Gly Thr Gln Gly Asn Tyr Asp Asp 
        35                  40                  45              


Asp Trp Lys Glu Phe Tyr Ser Thr Asp Asn Lys Tyr Asp Ala Ala Gly 
    50                  55                  60                  


Tyr Ser Val Asp Asn Glu Asn Pro Leu Ser Gly Lys Ala Gly Gly Val 
65                  70                  75                  80  


Val Lys Val Thr Tyr Pro Gly Leu Thr Lys Val Leu Ala Leu Lys Val 
                85                  90                  95      


Asp Asn Ala Glu Thr Ile Lys Lys Glu Leu Gly Leu Ser Leu Thr Glu 
            100                 105                 110         


Pro Leu Met Glu Gln Val Gly Thr Glu Glu Phe Ile Lys Arg Phe Gly 
        115                 120                 125             


Asp Gly Ala Ser Arg Val Val Leu Ser Leu Pro Phe Ala Glu Gly Ser 
    130                 135                 140                 


Ser Ser Val Glu Tyr Ile Asn Asn Trp Glu Gln Ala Lys Ala Leu Ser 
145                 150                 155                 160 


Val Glu Leu Glu Ile Asn Phe Glu Thr Arg Gly Lys Arg Gly Gln Asp 
                165                 170                 175     


Ala Met Tyr Glu Tyr Met Ala Gln Ala Cys Ala Gly Asn Arg Val Arg 
            180                 185                 190         


Arg Ser Val Gly Ser Ser Leu Ser Cys Ile Asn Leu Asp Trp Asp Val 
        195                 200                 205             


Ile Arg Asp Lys Thr Lys Thr Lys Ile Glu Ser Leu Lys Glu His Gly 
    210                 215                 220                 


Pro Ile Lys Asn Lys Met Ser Glu Ser Pro Asn Lys Thr Val Ser Glu 
225                 230                 235                 240 


Glu Lys Ala Lys Gln Tyr Leu Glu Glu Phe His Gln Thr Ala Leu Glu 
                245                 250                 255     


His Pro Glu Leu Ser Glu Leu Lys Thr Val Thr Gly Thr Asn Pro Val 
            260                 265                 270         


Phe Ala Gly Ala Asn Tyr Ala Ala Trp Ala Val Asn Val Ala Gln Val 
        275                 280                 285             


Ile Asp Ser Glu Thr Ala Asp Asn Leu Glu Lys Thr Thr Ala Ala Leu 
    290                 295                 300                 


Ser Ile Leu Pro Gly Ile Gly Ser Val Met Gly Ile Ala Asp Gly Ala 
305                 310                 315                 320 


Val His His Asn Thr Glu Glu Ile Val Ala Gln Ser Ile Ala Leu Ser 
                325                 330                 335     


Ser Leu Met Val Ala Gln Ala Ile Pro Leu Val Gly Glu Leu Val Asp 
            340                 345                 350         


Ile Gly Phe Ala Ala Tyr Asn Phe Val Glu Ser Ile Ile Asn Leu Phe 
        355                 360                 365             


Gln Val Val His Asn Ser Tyr Asn Arg Pro Ala Tyr Ser Pro Gly His 
    370                 375                 380                 


Lys Thr Gln Pro Phe Leu His Asp Gly Tyr Ala Val Ser Trp Asn Thr 
385                 390                 395                 400 


Val Glu Asp Ser Ile Ile Arg Thr Gly Phe Gln Gly Glu Ser Gly His 
                405                 410                 415     


Asp Ile Lys Ile Thr Ala Glu Asn Thr Pro Leu Pro Ile Ala Gly Val 
            420                 425                 430         


Leu Leu Pro Thr Ile Pro Gly Lys Leu Asp Val Asn Lys Ser Lys Thr 
        435                 440                 445             


His Ile Ser Val Asn Gly Arg Lys Ile Arg Met Arg Cys Arg Ala Ile 
    450                 455                 460                 


Asp Gly Asp Val Thr Phe Cys Arg Pro Lys Ser Pro Val Tyr Val Gly 
465                 470                 475                 480 


Asn Gly Val His Ala Asn Leu His Val Ala Phe His Arg Ser Ser Ser 
                485                 490                 495     


Glu Lys Ile His Ser Asn Glu Ile Ser Ser Asp Ser Ile Gly Val Leu 
            500                 505                 510         


Gly Tyr Gln Lys Thr Val Asp His Thr Lys Val Asn Ser Lys Leu Ser 
        515                 520                 525             


Leu Phe Phe Glu Ile Lys Ser 
    530                 535 


<210>  3
<211>  1611
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRM197 sequence optimized for expression in cytoplasm

<400>  3
atgggcgcag atgacgtagt agacagcagc aaaagcttcg tgatggaaaa ctttagctcg       60

taccacggta cgaagccagg ttatgtcgac agcattcaaa aaggtatcca gaaaccgaag      120

tccggcacgc agggtaacta cgacgacgat tggaaagagt tctacagcac cgacaacaag      180

tatgacgcag cgggttacag cgttgacaat gagaatccgt tgagcggcaa agcgggtggt      240

gttgtcaaag tgacgtatcc gggtctgacc aaggtcctgg cgttgaaagt tgataacgcg      300

gaaaccatta agaaagagct gggtctgagc ctgaccgagc cgttgatgga gcaagttggt      360

accgaagagt ttatcaaacg tttcggcgat ggtgcgagcc gcgttgtcct gtccctgcct      420

ttcgcggagg gcagctccag cgttgagtat atcaataact gggagcaagc aaaagcgctg      480

tccgtcgaac tggaaatcaa ttttgaaacg cgcggtaaac gtggtcaaga tgcaatgtac      540

gagtatatgg cccaggcctg cgctggtaat cgtgttcgtc gcagcgttgg tagcagcttg      600

tcttgtatca acctggattg ggatgtgatc cgtgataaga ccaagactaa gatcgagagc      660

ctgaaagaac atggcccgat taagaacaag atgtcggaga gcccgaataa gaccgtgagc      720

gaagaaaagg ccaagcagta tctggaagag ttccaccaaa cggctctgga gcatccggag      780

ctgagcgagc tgaaaacggt tacgggcacc aacccggtgt tcgcaggtgc gaattacgcg      840

gcgtgggcag tgaatgtggc gcaggtcatc gactccgaaa cggcggacaa tttggagaaa      900

accaccgcag cgctgagcat tctgccgggc atcggcagcg ttatgggcat tgcagatggt      960

gctgtgcacc ataacactga agaaatcgta gcgcaaagca ttgccctgtc tagcttgatg     1020

gtggcgcagg ctattccgct ggtcggcgaa ctggttgata tcggctttgc tgcctacaac     1080

ttcgttgaaa gcatcattaa cctgtttcag gtggtccaca acagctataa tcgcccagcg     1140

tacagcccgg gtcacaagac ccaaccgttc ctgcacgatg gctatgcggt gtcttggaac     1200

acggtcgaag atagcatcat tcgtaccggt ttccagggcg agagcggcca tgacatcaag     1260

attactgcag aaaatacccc gctgccgatc gcaggtgtcc tgctgcctac gattccgggt     1320

aagctggacg ttaacaaaag caaaacccac atttctgtga acggtcgtaa gattcgcatg     1380

cgttgtcgtg cgattgacgg cgacgtcacc ttctgccgtc cgaagagccc ggtctacgtt     1440

ggtaatggtg tgcacgcgaa cctgcacgtg gcgtttcacc gcagcagctc ggagaaaatc     1500

catagcaatg agatttctag cgacagcatt ggcgttctgg gttaccaaaa gacggttgac     1560

cataccaaag tcaattccaa actgagcctg ttctttgaga tcaaaagcta a              1611


<210>  4
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PelB signal sequence

<400>  4

Met Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala 
1               5                   10                  15      


Ala Gln Pro Ala Met Ala 
            20          


<210>  5
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OmpA signal sequence

<400>  5

Met Lys Lys Thr Ala Ile Ala Ile Ala Val Ala Leu Ala Gly Phe Ala 
1               5                   10                  15      


Thr Val Ala Gln Ala 
            20      


<210>  6
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  StII signal sequence

<400>  6

Met Lys Lys Asn Ile Ala Phe Leu Leu Ala Ser Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Tyr Ala 
            20              


<210>  7
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Endoxylanase signal sequence

<400>  7

Met Phe Lys Phe Lys Lys Lys Phe Leu Val Gly Leu Thr Ala Ala Phe 
1               5                   10                  15      


Met Ser Ile Ser Met Phe Ser Ala Thr Ala Ser Ala 
            20                  25              


<210>  8
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PhoA signal sequence

<400>  8

Met Lys Gln Ser Thr Ile Ala Leu Ala Leu Leu Pro Leu Leu Phe Thr 
1               5                   10                  15      


Pro Val Thr Lys Ala 
            20      


<210>  9
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OmpF signal sequence

<400>  9

Met Met Lys Arg Asn Ile Leu Ala Val Ile Val Pro Ala Leu Leu Val 
1               5                   10                  15      


Ala Gly Thr Ala Asn Ala 
            20          


<210>  10
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PhoE signal sequence

<400>  10

Met Lys Lys Ser Thr Leu Ala Leu Val Val Met Gly Ile Val Ala Ser 
1               5                   10                  15      


Ala Ser Val Gln Ala 
            20      


<210>  11
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MalE signal sequence

<400>  11

Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr 
1               5                   10                  15      


Thr Met Met Phe Ser Ala Ser Ala Leu Ala 
            20                  25      


<210>  12
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OmpC signal sequence

<400>  12

Met Lys Val Lys Val Leu Ser Leu Leu Val Pro Ala Leu Leu Val Ala 
1               5                   10                  15      


Gly Ala Ala Asn Ala 
            20      


<210>  13
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Lpp signal sequence

<400>  13

Met Lys Ala Thr Lys Leu Val Leu Gly Ala Val Ile Leu Gly Ser Thr 
1               5                   10                  15      


Leu Leu Ala Gly 
            20  


<210>  14
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LamB signal sequence

<400>  14

Met Met Ile Thr Leu Arg Lys Leu Pro Leu Ala Val Ala Val Ala Ala 
1               5                   10                  15      


Gly Val Met Ser Ala Gln Ala Met Ala 
            20                  25  


<210>  15
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OmpT signal sequence

<400>  15

Met Arg Ala Lys Leu Leu Gly Ile Val Leu Thr Thr Pro Ile Ala Ile 
1               5                   10                  15      


Ser Ser Phe Ala 
            20  


<210>  16
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LTB signal sequence

<400>  16

Met Asn Lys Val Lys Cys Tyr Val Leu Phe Thr Ala Leu Leu Ser Ser 
1               5                   10                  15      


Leu Tyr Ala His Gly 
            20      


<210>  17
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MglB signal sequence

<400>  17

Met Asn Lys Lys Val Leu Thr Leu Ser Ala Val Met Ala Ser Met Leu 
1               5                   10                  15      


Phe Gly Ala Ala Ala His Ala 
            20              


<210>  18
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  OppA signal sequence

<400>  18

Met Thr Asn Ile Thr Lys Arg Ser Leu Val Ala Ala Gly Val Leu Ala 
1               5                   10                  15      


Ala Leu Met Ala Gly Asn Val Ala Leu Ala 
            20                  25      


<210>  19
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RbsB signal sequence

<400>  19

Met Asn Met Lys Lys Leu Ala Thr Leu Val Ser Ala Val Ala Leu Ser 
1               5                   10                  15      


Ala Thr Val Ser Ala Asn Ala Met Ala 
            20                  25  


<210>  20
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Agp signal sequence

<400>  20

Met Asn Lys Thr Leu Ile Ala Ala Ala Val Ala Gly Ile Val Leu Leu 
1               5                   10                  15      


Ala Ser Asn Ala Gln Ala 
            20          


<210>  21
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FkpA signal sequence

<400>  21

Met Lys Ser Leu Phe Lys Val Thr Leu Leu Ala Thr Thr Met Ala Val 
1               5                   10                  15      


Ala Leu His Ala Pro Ile Thr Phe Ala 
            20                  25  


<210>  22
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  YtfQ signal sequence

<400>  22

Met Trp Lys Arg Leu Leu Ile Val Ser Ala Val Ser Ala Ala Met Ser 
1               5                   10                  15      


Ser Met Ala Leu Ala 
            20      


<210>  23
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HdeA signal sequence

<400>  23

Met Lys Lys Val Leu Gly Val Ile Leu Gly Gly Leu Leu Leu Leu Pro 
1               5                   10                  15      


Val Val Ser Asn Ala 
            20      


<210>  24
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HdeB signal sequence

<400>  24

Met Asn Ile Ser Ser Leu Arg Lys Ala Phe Ile Phe Met Gly Ala Val 
1               5                   10                  15      


Ala Ala Leu Ser Leu Val Asn Ala Gln Ser Ala Leu Ala 
            20                  25                  


<210>  25
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GlnH signal sequence

<400>  25

Met Lys Ser Val Leu Lys Val Ser Leu Ala Ala Leu Thr Leu Ala Phe 
1               5                   10                  15      


Ala Val Ser Ser His Ala 
            20          


<210>  26
<211>  1743
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pelB-CRM197 fusion with 3' and 5' flanking sequence

<400>  26
cctctagaaa taattttgtt taacttttga aggagatata catatgaaat acttgctgcc       60

aaccgccgcc gccggcctgc tgctgctcgc agcacagccg gctatggcag gtgcggatga      120

tgttgtggac agctctaagt cttttgtgat ggaaaacttt agctcgtacc acggtacgaa      180

gccaggttat gtcgacagca ttcaaaaagg tatccagaaa ccgaagtccg gcacgcaggg      240

taactacgac gacgattgga aagagttcta cagcaccgac aacaagtatg acgcagcggg      300

ttacagcgtt gacaatgaga atccgttgag cggcaaagcg ggtggtgttg tcaaagtgac      360

gtatccgggt ctgaccaagg tcctggcgtt gaaagttgat aacgcggaaa ccattaagaa      420

agagctgggt ctgagcctga ccgagccgtt gatggagcaa gttggtaccg aagagtttat      480

caaacgtttc ggcgatggtg cgagccgcgt tgtcctgtcc ctgcctttcg cggagggcag      540

ctccagcgtt gagtatatca ataactggga gcaagcaaaa gcgctgtccg tcgaactgga      600

aatcaatttt gaaacgcgcg gtaaacgtgg tcaagatgca atgtacgagt atatggccca      660

ggcctgcgct ggtaatcgtg ttcgtcgcag cgttggtagc agcttgtctt gtatcaacct      720

ggattgggat gtgatccgtg ataagaccaa gactaagatc gagagcctga aagaacatgg      780

cccgattaag aacaagatgt cggagagccc gaataagacc gtgagcgaag aaaaggccaa      840

gcagtatctg gaagagttcc accaaacggc tctggagcat ccggagctga gcgagctgaa      900

aacggttacg ggcaccaacc cggtgttcgc aggtgcgaat tacgcggcgt gggcagtgaa      960

tgtggcgcag gtcatcgact ccgaaacggc ggacaatttg gagaaaacca ccgcagcgct     1020

gagcattctg ccgggcatcg gcagcgttat gggcattgca gatggtgctg tgcaccataa     1080

cactgaagaa atcgtagcgc aaagcattgc cctgtctagc ttgatggtgg cgcaggctat     1140

tccgctggtc ggcgaactgg ttgatatcgg ctttgctgcc tacaacttcg ttgaaagcat     1200

cattaacctg tttcaggtgg tccacaacag ctataatcgc ccagcgtaca gcccgggtca     1260

caagacccaa ccgttcctgc acgatggcta tgcggtgtct tggaacacgg tcgaagatag     1320

catcattcgt accggtttcc agggcgagag cggccatgac atcaagatta ctgcagaaaa     1380

taccccgctg ccgatcgcag gtgtcctgct gcctacgatt ccgggtaagc tggacgttaa     1440

caaaagcaaa acccacattt ctgtgaacgg tcgtaagatt cgcatgcgtt gtcgtgcgat     1500

tgacggcgac gtcaccttct gccgtccgaa gagcccggtc tacgttggta atggtgtgca     1560

cgcgaacctg cacgtggcgt ttcaccgcag cagctcggag aaaatccata gcaatgagat     1620

ttctagcgac agcattggcg ttctgggtta ccaaaagacg gttgaccata ccaaagtcaa     1680

ttccaaactg agcctgttct ttgagatcaa aagctaactc gagccccaag ggcgacaccc     1740

cct                                                                   1743


<210>  27
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR sense primer (pelB-CRM197)

<400>  27
ggagatatac atatgaaata cttgctgcca acc                                    33


<210>  28
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR antisense primer (PelB-CRM197)

<400>  28
ctttgttagc agccgattag cttttgatct caaagaaca                              39


<210>  29
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR sense primer (OmpA-CRM197)

<400>  29
gctaccgtag cgcaggccgg tgcggatgat gttgtgga                               38


<210>  30
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR antisense primer (OmpA-CRM197)

<400>  30
ctttgttagc agccgattag cttttgatct caaagaaca                              39


<210>  31
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR sense primer 2 (OmpA-CRM197)

<400>  31
ggagatatac atatgaaaaa gacagctatc gcgattgcag tggcactggc tggtttcgct       60

accgtagcgc aggcc                                                        75


<210>  32
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR antisense primer 2 (OmpA-CRM197)

<400>  32
ctttgttagc agccgattag cttttgatct caaagaaca                              39


<210>  33
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR sense primer 3 (OmpA-CRM197)

<400>  33
ggagatatac atatgaaaaa gacagctatc g                                      31


<210>  34
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR antisense primer 3 (OmpA-CRM197)

<400>  34
ctttgttagc agccgattag cttttgatct caaagaaca                              39


<210>  35
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR sense primer (OmpF-CRM197)

<400>  35
gttagtagca ggtactgcaa acgctggtgc ggatgatgtt gtgga                       45


<210>  36
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR antisense primer (OmpF-CRM197)

<400>  36
ctttgttagc agccgattag cttttgatct caaagaaca                              39


<210>  37
<211>  78
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR sense primer 2 (OmpF-CRM197)

<400>  37
ggagatatac atatgatgaa gcgcaatatt ctggcagtga tcgtccctgc tctgttagta       60

gcaggtactg caaacgct                                                     78


<210>  38
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR antisense primer 2 (OmpF-CRM197)

<400>  38
ctttgttagc agccgattag cttttgatct caaagaaca                              39


