                         SEQUENCE LISTING

<110>  UCL Business PLC
 
<120>  CHIMERIC ANTIGEN RECEPTOR

<130>  P103293PCT

<150>  1403972.1
<151>  2014-03-06

<160>  37    

<170>  PatentIn version 3.5

<210>  1
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heavy chain variable region CDR1

<400>  1

Ser Tyr Asn Ile His 
1               5   


<210>  2
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heavy chain variable region CDR2

<400>  2

Val Ile Trp Ala Gly Gly Ser Thr Asn Tyr Asn Ser Ala Leu Met Ser 
1               5                   10                  15      


<210>  3
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heavy chain variable region CDR3

<400>  3

Arg Ser Asp Asp Tyr Ser Trp Phe Ala Tyr 
1               5                   10  


<210>  4
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Light chain variable region CDR1

<400>  4

Arg Ala Ser Ser Ser Val Ser Ser Ser Tyr Leu His 
1               5                   10          


<210>  5
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Light chain variable region CDR2

<400>  5

Ser Thr Ser Asn Leu Ala Ser 
1               5           


<210>  6
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Light chain variable region CDR3

<400>  6

Gln Gln Tyr Ser Gly Tyr Pro Ile Thr 
1               5                   


<210>  7
<211>  243
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Murine KM666 sequence

<400>  7

Gln Val Gln Leu Lys Glu Ser Gly Pro Val Leu Val Ala Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Ala Ser Tyr 
            20                  25                  30          


Asn Ile His Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Gly Val Ile Trp Ala Gly Gly Ser Thr Asn Tyr Asn Ser Ala Leu Met 
    50                  55                  60                  


Ser Arg Leu Ser Ile Ser Lys Asp Asn Ser Lys Ser Gln Val Phe Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Met Tyr Tyr Cys Ala 
                85                  90                  95      


Lys Arg Ser Asp Asp Tyr Ser Trp Phe Ala Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ala Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Glu Asn Val Leu Thr Gln Ser Pro Ala Ile 
    130                 135                 140                 


Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser 
145                 150                 155                 160 


Ser Ser Val Ser Ser Ser Tyr Leu His Trp Tyr Gln Gln Lys Ser Gly 
                165                 170                 175     


Ala Ser Pro Lys Val Trp Ile Tyr Ser Thr Ser Asn Leu Ala Ser Gly 
            180                 185                 190         


Val Pro Gly Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu 
        195                 200                 205             


Thr Ile Ser Ser Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln 
    210                 215                 220                 


Gln Tyr Ser Gly Tyr Pro Ile Thr Phe Gly Ala Gly Thr Lys Val Glu 
225                 230                 235                 240 


Val Lys Arg 
            


<210>  8
<211>  242
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanised KM666 sequence

<400>  8

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Ala Ser Tyr 
            20                  25                  30          


Asn Ile His Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Gly Val Ile Trp Ala Gly Gly Ser Thr Asn Tyr Asn Ser Ala Leu Met 
    50                  55                  60                  


Ser Arg Leu Thr Ile Ser Lys Asp Asn Ser Lys Asn Gln Val Phe Leu 
65                  70                  75                  80  


Lys Met Ser Ser Leu Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Lys Arg Ser Asp Asp Tyr Ser Trp Phe Ala Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        115                 120                 125             


Gly Gly Gly Gly Ser Glu Asn Gln Met Thr Gln Ser Pro Ser Ser Leu 
    130                 135                 140                 


Ser Ala Ser Val Gly Asp Arg Val Thr Met Thr Cys Arg Ala Ser Ser 
145                 150                 155                 160 


Ser Val Ser Ser Ser Tyr Leu His Trp Tyr Gln Gln Lys Ser Gly Lys 
                165                 170                 175     


Ala Pro Lys Val Trp Ile Tyr Ser Thr Ser Asn Leu Ala Ser Gly Val 
            180                 185                 190         


Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr 
        195                 200                 205             


Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln 
    210                 215                 220                 


Tyr Ser Gly Tyr Pro Ile Thr Phe Gly Gln Gly Thr Lys Val Glu Ile 
225                 230                 235                 240 


Lys Arg 
        


<210>  9
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Murine KM666 VH (heavy chain variable region) sequence

<400>  9

Gln Val Gln Leu Lys Glu Ser Gly Pro Val Leu Val Ala Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Ala Ser Tyr 
            20                  25                  30          


Asn Ile His Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Gly Val Ile Trp Ala Gly Gly Ser Thr Asn Tyr Asn Ser Ala Leu Met 
    50                  55                  60                  


Ser Arg Leu Ser Ile Ser Lys Asp Asn Ser Lys Ser Gln Val Phe Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Met Tyr Tyr Cys Ala 
                85                  90                  95      


Lys Arg Ser Asp Asp Tyr Ser Trp Phe Ala Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ala 
        115             


<210>  10
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanised KM666 VH sequence

<400>  10

Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser Leu Ala Ser Tyr 
            20                  25                  30          


Asn Ile His Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Gly Val Ile Trp Ala Gly Gly Ser Thr Asn Tyr Asn Ser Ala Leu Met 
    50                  55                  60                  


Ser Arg Leu Thr Ile Ser Lys Asp Asn Ser Lys Asn Gln Val Phe Leu 
65                  70                  75                  80  


Lys Met Ser Ser Leu Thr Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Lys Arg Ser Asp Asp Tyr Ser Trp Phe Ala Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ser 
        115             


<210>  11
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Murine KM666 VL (light chain variable region) sequence

<400>  11

Glu Asn Val Leu Thr Gln Ser Pro Ala Ile Met Ser Ala Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Thr Cys Arg Ala Ser Ser Ser Val Ser Ser Ser 
            20                  25                  30          


Tyr Leu His Trp Tyr Gln Gln Lys Ser Gly Ala Ser Pro Lys Val Trp 
        35                  40                  45              


Ile Tyr Ser Thr Ser Asn Leu Ala Ser Gly Val Pro Gly Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Ser Val Glu 
65                  70                  75                  80  


Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Gly Tyr Pro 
                85                  90                  95      


Ile Thr Phe Gly Ala Gly Thr Lys Val Glu Val Lys 
            100                 105             


<210>  12
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Humanised KM666 VL sequence

<400>  12

Glu Asn Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Met Thr Cys Arg Ala Ser Ser Ser Val Ser Ser Ser 
            20                  25                  30          


Tyr Leu His Trp Tyr Gln Gln Lys Ser Gly Lys Ala Pro Lys Val Trp 
        35                  40                  45              


Ile Tyr Ser Thr Ser Asn Leu Ala Ser Gly Val Pro Ser Arg Phe Ser 
    50                  55                  60                  


Gly Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser Leu Gln 
65                  70                  75                  80  


Pro Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Gly Tyr Pro 
                85                  90                  95      


Ile Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105             


<210>  13
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Transmembrane domain

<400>  13

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  14
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 endodomain

<400>  14

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr 
        35                  


<210>  15
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD40 endodomain

<400>  15

Arg Ser Arg Asp Gln Arg Leu Pro Pro Asp Ala His Lys Pro Pro Gly 
1               5                   10                  15      


Gly Gly Ser Phe Arg Thr Pro Ile Gln Glu Glu Gln Ala Asp Ala His 
            20                  25                  30          


Ser Thr Leu Ala Lys Ile 
        35              


<210>  16
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3 zeta endodomain

<400>  16

Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln 
1               5                   10                  15      


Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu 
            20                  25                  30          


Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly 
        35                  40                  45              


Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu 
    50                  55                  60                  


Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly 
65                  70                  75                  80  


Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
                85                  90                  95      


Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro 
            100                 105                 110         


Pro Arg 
        


<210>  17
<211>  153
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28Z

<400>  17

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser 
        35                  40                  45              


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
    50                  55                  60                  


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
65                  70                  75                  80  


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
                85                  90                  95      


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
            100                 105                 110         


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
        115                 120                 125             


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
    130                 135                 140                 


Leu His Met Gln Ala Leu Pro Pro Arg 
145                 150             


<210>  18
<211>  189
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28OXZ

<400>  18

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Asp Gln Arg Leu Pro Pro 
        35                  40                  45              


Asp Ala His Lys Pro Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile Gln 
    50                  55                  60                  


Glu Glu Gln Ala Asp Ala His Ser Thr Leu Ala Lys Ile Arg Val Lys 
65                  70                  75                  80  


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
                85                  90                  95      


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
            100                 105                 110         


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
        115                 120                 125             


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
    130                 135                 140                 


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
145                 150                 155                 160 


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
                165                 170                 175     


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            180                 185                 


<210>  19
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Signal peptide

<400>  19

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly 
            20  


<210>  20
<211>  234
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge-CH2CH3 of human IgG1 spacer

<400>  20

Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15      


Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Thr Leu Met Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    130                 135                 140                 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 


Ser Leu Ser Leu Ser Pro Gly Lys Lys Asp 
225                 230                 


<210>  21
<211>  46
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD8 stalk spacer

<400>  21

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile 
        35                  40                  45      


<210>  22
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 hinge spacer

<400>  22

Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15      


Lys Asp Pro Lys 
            20  


<210>  23
<211>  237
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG1 Hinge-Fc spacer

<400>  23

Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15      


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
            20                  25                  30          


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
        35                  40                  45              


Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr 
    50                  55                  60                  


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
65                  70                  75                  80  


Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
                85                  90                  95      


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
            100                 105                 110         


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln 
        115                 120                 125             


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu 
    130                 135                 140                 


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
145                 150                 155                 160 


Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn 
                165                 170                 175     


Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu 
            180                 185                 190         


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val 
        195                 200                 205             


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
    210                 215                 220                 


Lys Ser Leu Ser Leu Ser Pro Gly Lys Lys Asp Pro Lys 
225                 230                 235         


<210>  24
<211>  236
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG1 Hinge spacer - Fc modified to remove Fc receptor recognition
       motifs

<400>  24

Ala Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15      


Ala Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Thr Leu Met Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    130                 135                 140                 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 


Ser Leu Ser Leu Ser Pro Gly Lys Lys Asp Pro Lys 
225                 230                 235     


<210>  25
<211>  6014
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Retroviral cassette

<400>  25
tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat       60

ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca gatggaacag      120

ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa      180

gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc      240

ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag      300

agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg      360

aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttatgctccc cgagctcaat      420

aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg      480

tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct      540

tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct      600

cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg      660

gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct      720

gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga      780

gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg      840

tggcccgacc tgagtcctaa aatcccgatc gtttaggact ctttggtgca ccccccttag      900

aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg      960

aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg     1020

ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatatg ggcccgggct     1080

agcctgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg     1140

ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat     1200

ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc     1260

aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtggggtaca     1320

tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc     1380

ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt     1440

cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat     1500

ggccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct gaccctgaca     1560

tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc     1620

agcacgaagt ctggagacct ctggcggcag cctaccaaga acaactggac cgaccggtgg     1680

tacctcaccc ttaccgagtc ggcgacacag tgtgggtccg ccgacaccag actaagaacc     1740

tagaacctcg ctggaaagga ccttacacag tcctgctgac cacccccacc gccctcaaag     1800

tagacggcat cgcagcttgg atacacgccg cccacgtgaa ggctgccgac cccgggggtg     1860

gaccatcctc tagactgcca acatgggcac cagcctgctg tgctggatgg ccctgtgcct     1920

gctgggcgcc gaccacgccg atgcctgccc ctacagcaac cccagcctgt gcagcggagg     1980

cggcggcagc gagctgccca cccagggcac cttctccaac gtgtccacca acgtgagccc     2040

agccaagccc accaccaccg cctgtcctta ttccaatcct tccctgtgta gcggaggggg     2100

aggcagccca gcccccagac ctcccacccc agcccccacc atcgccagcc agcctctgag     2160

cctgagaccc gaggcctgcc gcccagccgc cggcggcgcc gtgcacacca gaggcctgga     2220

tttcgcctgc gatatctaca tctgggcccc actggccggc acctgtggcg tgctgctgct     2280

gagcctggtg atcaccctgt actgcaacca ccgcaaccgc aggcgcgtgt gcaagtgccc     2340

caggcccgtg gtgagagccg agggcagagg cagcctgctg acctgcggcg acgtggagga     2400

gaacccaggc cccatggaga ccgacaccct gctgctgtgg gtgctgctgc tgtgggtgcc     2460

aggcagcacc ggccaggtgc agctgcagga gtctggccca ggcctggtga agcccagcca     2520

gaccctgagc atcacctgca ccgtgagcgg cttcagcctg gccagctaca acatccactg     2580

ggtgcggcag cccccaggca agggcctgga gtggctgggc gtgatctggg ctggcggcag     2640

caccaactac aacagcgccc tgatgagccg gctgaccatc agcaaggaca acagcaagaa     2700

ccaggtgttc ctgaagatga gcagcctgac agccgccgac accgccgtgt actactgcgc     2760

caagcggagc gacgactaca gctggttcgc ctactggggc cagggcaccc tggtgaccgt     2820

gagctctggc ggaggcggct ctggcggagg cggctctggc ggaggcggca gcgagaacca     2880

gatgacccag agccccagca gcttgagcgc cagcgtgggc gaccgggtga ccatgacctg     2940

cagagccagc agcagcgtga gcagcagcta cctgcactgg taccagcaga agagcggcaa     3000

ggccccaaag gtgtggatct acagcaccag caacctggcc agcggcgtgc ccagccggtt     3060

cagcggcagc ggcagcggca ccgactacac cctgaccatc agcagcctgc agcccgagga     3120

cttcgccacc tactactgcc agcagtacag cggctacccc atcaccttcg gccagggcac     3180

caaggtggag atcaagcggt cggatcccgc cgagcccaaa tctcctgaca aaactcacac     3240

atgcccaccg tgcccagcac ctcccgtggc cggcccgtca gtcttcctct tccccccaaa     3300

acccaaggac accctcatga tcgcccggac ccctgaggtc acatgcgtgg tggtggacgt     3360

gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg gacggcgtgg aggtgcataa     3420

tgccaagaca aagccgcggg aggagcagta caacagcacg taccgtgtgg tcagcgtcct     3480

caccgtcctg caccaggact ggctgaatgg caaggagtac aagtgcaagg tctccaacaa     3540

agccctccca gcccccatcg agaaaaccat ctccaaagcc aaagggcagc cccgagaacc     3600

acaggtgtac accctgcccc catcccggga tgagctgacc aagaaccagg tcagcctgac     3660

ctgcctggtc aaaggcttct atcccagcga catcgccgtg gagtgggaga gcaatgggca     3720

accggagaac aactacaaga ccacgcctcc cgtgctggac tccgacggct ccttcttcct     3780

ctacagcaag ctcaccgtgg acaagagcag gtggcagcag gggaacgtct tctcatgctc     3840

cgtgatgcat gaggccctgc acaatcacta tacccagaaa tctctgagtc tgagcccagg     3900

caagaaggac cccaagttct gggtcctggt ggtggtggga ggcgtgctgg cctgttactc     3960

tctcctggtg accgtggcct tcatcatctt ctgggtgcgc tccaagagga gcaggctcct     4020

gcacagtgac tacatgaaca tgactccccg ccgccccggg cccacccgca agcattacca     4080

gccctatgcc ccaccacgcg acttcgcagc ctatcgctcc cgggtgaagt tctctcgctc     4140

tgccgatgcc ccagcctatc agcagggcca gaatcagctg tacaatgaac tgaacctggg     4200

caggcgggag gagtacgacg tgctggataa gcggagaggc agagaccccg agatgggcgg     4260

caaaccacgg cgcaaaaatc cccaggaggg actctataac gagctgcaga aggacaaaat     4320

ggccgaggcc tattccgaga tcggcatgaa gggagagaga agacgcggaa agggccacga     4380

cggcctgtat cagggattgt ccaccgctac aaaagataca tatgatgccc tgcacatgca     4440

ggccctgcca cccagatgac gcgtatcgat actgttctca tcacatcata tcaaggttat     4500

ataccatcaa tattgccaca gatgttactt agccttttaa tatttctcta atttagtgta     4560

tatgcaatga tagttctctg atttctgaga ttgagtttct catgtgtaat gattatttag     4620

agtttctctt tcatctgttc aaatttttgt ctagttttat tttttactga tttgtaagac     4680

ttctttttat aatctgcata ttacaattct ctttactggg gtgttgcaaa tattttctgt     4740

cattctatgg cctgactttt cttaatggtt ttttaatttt aaaaataagt cttaatattc     4800

atgcaatcta attaacaatc ttttctttgt ggttaggact ttgagtcata agaaattttt     4860

ctctacactg aagtcatgat ggcatgcttc tatattattt tctaaaagat ttaaagtttt     4920

gccttctcca tttagactta taattcactg gaattttttt gtgtgtatgg tatgacatat     4980

gggttccctt ttatttttta catataaata tatttccctg tttttctaaa aaagaaaaag     5040

atcatcattt tcccattgta aaatgccata tttttttcat aggtcactta catatatcaa     5100

tgggtctgtt tctgagctct actctatttt atcagcctca ctgtctatcc ccacacatct     5160

catgctttgc tctaaatctt gatatttagt ggaacattct ttcccatttt gttctacaag     5220

aatatttttg ttattgtctt tgggctttct atatacattt tgaaatgagg ttgacaagtt     5280

cggattagtc caatttgtta aagacaggat atcagtggtc caggctctag ttttgactca     5340

acaatatcac cagctgaagc ctatagagta cgagccatag ataaaataaa agattttatt     5400

tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta     5460

agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaga agttcagatc     5520

aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag     5580

ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat     5640

atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg     5700

gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct     5760

gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg     5820

cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc     5880

tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc     5940

cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag     6000

cgggggtctt tcac                                                       6014


<210>  26
<211>  719
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-GD2 CAR, muKM666-HCH2CH3-CD28OXZ

<400>  26

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Gln Val Gln Leu Lys Glu Ser Gly Pro Val Leu Val 
            20                  25                  30          


Ala Pro Ser Gln Thr Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser 
        35                  40                  45              


Leu Ala Ser Tyr Asn Ile His Trp Val Arg Gln Pro Pro Gly Lys Gly 
    50                  55                  60                  


Leu Glu Trp Leu Gly Val Ile Trp Ala Gly Gly Ser Thr Asn Tyr Asn 
65                  70                  75                  80  


Ser Ala Leu Met Ser Arg Leu Ser Ile Ser Lys Asp Asn Ser Lys Ser 
                85                  90                  95      


Gln Val Phe Leu Gln Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Met 
            100                 105                 110         


Tyr Tyr Cys Ala Lys Arg Ser Asp Asp Tyr Ser Trp Phe Ala Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Leu Val Thr Val Ser Ala Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Asn Val Leu Thr Gln 
145                 150                 155                 160 


Ser Pro Ala Ile Met Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr 
                165                 170                 175     


Cys Arg Ala Ser Ser Ser Val Ser Ser Ser Tyr Leu His Trp Tyr Gln 
            180                 185                 190         


Gln Lys Ser Gly Ala Ser Pro Lys Val Trp Ile Tyr Ser Thr Ser Asn 
        195                 200                 205             


Leu Ala Ser Gly Val Pro Gly Arg Phe Ser Gly Ser Gly Ser Gly Thr 
    210                 215                 220                 


Ser Tyr Ser Leu Thr Ile Ser Ser Val Glu Ala Glu Asp Ala Ala Thr 
225                 230                 235                 240 


Tyr Tyr Cys Gln Gln Tyr Ser Gly Tyr Pro Ile Thr Phe Gly Ala Gly 
                245                 250                 255     


Thr Lys Val Glu Val Lys Arg Ser Asp Pro Ala Glu Pro Lys Ser Pro 
            260                 265                 270         


Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
        275                 280                 285             


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
    290                 295                 300                 


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
305                 310                 315                 320 


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
                325                 330                 335     


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
            340                 345                 350         


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
        355                 360                 365             


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
    370                 375                 380                 


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
385                 390                 395                 400 


Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 
                405                 410                 415     


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
            420                 425                 430         


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
        435                 440                 445             


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
    450                 455                 460                 


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
465                 470                 475                 480 


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
                485                 490                 495     


Pro Gly Lys Lys Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly 
            500                 505                 510         


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 
        515                 520                 525             


Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn 
    530                 535                 540                 


Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr 
545                 550                 555                 560 


Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Asp Gln Arg Leu 
                565                 570                 575     


Pro Pro Asp Ala His Lys Pro Pro Gly Gly Gly Ser Phe Arg Thr Pro 
            580                 585                 590         


Ile Gln Glu Glu Gln Ala Asp Ala His Ser Thr Leu Ala Lys Ile Arg 
        595                 600                 605             


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln 
    610                 615                 620                 


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
625                 630                 635                 640 


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
                645                 650                 655     


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
            660                 665                 670         


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
        675                 680                 685             


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
    690                 695                 700                 


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
705                 710                 715                 


<210>  27
<211>  718
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-GD2 CAR, huKM666-HCH2CH3-CD28OXZ

<400>  27

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val 
            20                  25                  30          


Lys Pro Ser Gln Thr Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser 
        35                  40                  45              


Leu Ala Ser Tyr Asn Ile His Trp Val Arg Gln Pro Pro Gly Lys Gly 
    50                  55                  60                  


Leu Glu Trp Leu Gly Val Ile Trp Ala Gly Gly Ser Thr Asn Tyr Asn 
65                  70                  75                  80  


Ser Ala Leu Met Ser Arg Leu Thr Ile Ser Lys Asp Asn Ser Lys Asn 
                85                  90                  95      


Gln Val Phe Leu Lys Met Ser Ser Leu Thr Ala Ala Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Lys Arg Ser Asp Asp Tyr Ser Trp Phe Ala Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Asn Gln Met Thr Gln Ser 
145                 150                 155                 160 


Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Met Thr Cys 
                165                 170                 175     


Arg Ala Ser Ser Ser Val Ser Ser Ser Tyr Leu His Trp Tyr Gln Gln 
            180                 185                 190         


Lys Ser Gly Lys Ala Pro Lys Val Trp Ile Tyr Ser Thr Ser Asn Leu 
        195                 200                 205             


Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Tyr Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
225                 230                 235                 240 


Tyr Cys Gln Gln Tyr Ser Gly Tyr Pro Ile Thr Phe Gly Gln Gly Thr 
                245                 250                 255     


Lys Val Glu Ile Lys Arg Ser Asp Pro Ala Glu Pro Lys Ser Pro Asp 
            260                 265                 270         


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
        275                 280                 285             


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
    290                 295                 300                 


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
305                 310                 315                 320 


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
                325                 330                 335     


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 
            340                 345                 350         


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
        355                 360                 365             


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
    370                 375                 380                 


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
385                 390                 395                 400 


Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu 
                405                 410                 415     


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
            420                 425                 430         


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
        435                 440                 445             


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
    450                 455                 460                 


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
465                 470                 475                 480 


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
                485                 490                 495     


Gly Lys Lys Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val 
            500                 505                 510         


Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp 
        515                 520                 525             


Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met 
    530                 535                 540                 


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
545                 550                 555                 560 


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Asp Gln Arg Leu Pro 
                565                 570                 575     


Pro Asp Ala His Lys Pro Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile 
            580                 585                 590         


Gln Glu Glu Gln Ala Asp Ala His Ser Thr Leu Ala Lys Ile Arg Val 
        595                 600                 605             


Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn 
    610                 615                 620                 


Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val 
625                 630                 635                 640 


Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg 
                645                 650                 655     


Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys 
            660                 665                 670         


Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg 
        675                 680                 685             


Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys 
    690                 695                 700                 


Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
705                 710                 715             


<210>  28
<211>  717
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-GD2 CAR, huKM666-HCH2CH3pvaa-CD28OXZ

<400>  28

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val 
            20                  25                  30          


Lys Pro Ser Gln Thr Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser 
        35                  40                  45              


Leu Ala Ser Tyr Asn Ile His Trp Val Arg Gln Pro Pro Gly Lys Gly 
    50                  55                  60                  


Leu Glu Trp Leu Gly Val Ile Trp Ala Gly Gly Ser Thr Asn Tyr Asn 
65                  70                  75                  80  


Ser Ala Leu Met Ser Arg Leu Thr Ile Ser Lys Asp Asn Ser Lys Asn 
                85                  90                  95      


Gln Val Phe Leu Lys Met Ser Ser Leu Thr Ala Ala Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Lys Arg Ser Asp Asp Tyr Ser Trp Phe Ala Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Asn Gln Met Thr Gln Ser 
145                 150                 155                 160 


Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Met Thr Cys 
                165                 170                 175     


Arg Ala Ser Ser Ser Val Ser Ser Ser Tyr Leu His Trp Tyr Gln Gln 
            180                 185                 190         


Lys Ser Gly Lys Ala Pro Lys Val Trp Ile Tyr Ser Thr Ser Asn Leu 
        195                 200                 205             


Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Tyr Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
225                 230                 235                 240 


Tyr Cys Gln Gln Tyr Ser Gly Tyr Pro Ile Thr Phe Gly Gln Gly Thr 
                245                 250                 255     


Lys Val Glu Ile Lys Arg Ser Asp Pro Ala Glu Pro Lys Ser Pro Asp 
            260                 265                 270         


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro 
        275                 280                 285             


Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ala 
    290                 295                 300                 


Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
305                 310                 315                 320 


Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
                325                 330                 335     


Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val 
            340                 345                 350         


Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
        355                 360                 365             


Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys 
    370                 375                 380                 


Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
385                 390                 395                 400 


Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr 
                405                 410                 415     


Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
            420                 425                 430         


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
        435                 440                 445             


Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys 
    450                 455                 460                 


Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
465                 470                 475                 480 


Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
                485                 490                 495     


Lys Lys Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val Leu 
            500                 505                 510         


Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
        515                 520                 525             


Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
    530                 535                 540                 


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
545                 550                 555                 560 


Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Asp Gln Arg Leu Pro Pro 
                565                 570                 575     


Asp Ala His Lys Pro Pro Gly Gly Gly Ser Phe Arg Thr Pro Ile Gln 
            580                 585                 590         


Glu Glu Gln Ala Asp Ala His Ser Thr Leu Ala Lys Ile Arg Val Lys 
        595                 600                 605             


Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
    610                 615                 620                 


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
625                 630                 635                 640 


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
                645                 650                 655     


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
            660                 665                 670         


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
        675                 680                 685             


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
    690                 695                 700                 


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
705                 710                 715         


<210>  29
<211>  527
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-GD2 CAR, huKM666-HSTK-CD28OXZ

<400>  29

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val 
            20                  25                  30          


Lys Pro Ser Gln Thr Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser 
        35                  40                  45              


Leu Ala Ser Tyr Asn Ile His Trp Val Arg Gln Pro Pro Gly Lys Gly 
    50                  55                  60                  


Leu Glu Trp Leu Gly Val Ile Trp Ala Gly Gly Ser Thr Asn Tyr Asn 
65                  70                  75                  80  


Ser Ala Leu Met Ser Arg Leu Thr Ile Ser Lys Asp Asn Ser Lys Asn 
                85                  90                  95      


Gln Val Phe Leu Lys Met Ser Ser Leu Thr Ala Ala Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Lys Arg Ser Asp Asp Tyr Ser Trp Phe Ala Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Asn Gln Met Thr Gln Ser 
145                 150                 155                 160 


Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Met Thr Cys 
                165                 170                 175     


Arg Ala Ser Ser Ser Val Ser Ser Ser Tyr Leu His Trp Tyr Gln Gln 
            180                 185                 190         


Lys Ser Gly Lys Ala Pro Lys Val Trp Ile Tyr Ser Thr Ser Asn Leu 
        195                 200                 205             


Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Tyr Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
225                 230                 235                 240 


Tyr Cys Gln Gln Tyr Ser Gly Tyr Pro Ile Thr Phe Gly Gln Gly Thr 
                245                 250                 255     


Lys Val Glu Ile Lys Arg Ser Asp Pro Thr Thr Thr Pro Ala Pro Arg 
            260                 265                 270         


Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg 
        275                 280                 285             


Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly 
    290                 295                 300                 


Leu Asp Phe Ala Cys Asp Ile Phe Trp Val Leu Val Val Val Gly Gly 
305                 310                 315                 320 


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 
                325                 330                 335     


Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn 
            340                 345                 350         


Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr 
        355                 360                 365             


Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Asp Gln Arg Leu 
    370                 375                 380                 


Pro Pro Asp Ala His Lys Pro Pro Gly Gly Gly Ser Phe Arg Thr Pro 
385                 390                 395                 400 


Ile Gln Glu Glu Gln Ala Asp Ala His Ser Thr Leu Ala Lys Ile Arg 
                405                 410                 415     


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln 
            420                 425                 430         


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
        435                 440                 445             


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
    450                 455                 460                 


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
465                 470                 475                 480 


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
                485                 490                 495     


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
            500                 505                 510         


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        515                 520                 525         


<210>  30
<211>  527
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-GD2 CAR, huKM666-STK-CD28XOXZ

<400>  30

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val 
            20                  25                  30          


Lys Pro Ser Gln Thr Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser 
        35                  40                  45              


Leu Ala Ser Tyr Asn Ile His Trp Val Arg Gln Pro Pro Gly Lys Gly 
    50                  55                  60                  


Leu Glu Trp Leu Gly Val Ile Trp Ala Gly Gly Ser Thr Asn Tyr Asn 
65                  70                  75                  80  


Ser Ala Leu Met Ser Arg Leu Thr Ile Ser Lys Asp Asn Ser Lys Asn 
                85                  90                  95      


Gln Val Phe Leu Lys Met Ser Ser Leu Thr Ala Ala Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Lys Arg Ser Asp Asp Tyr Ser Trp Phe Ala Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Asn Gln Met Thr Gln Ser 
145                 150                 155                 160 


Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Met Thr Cys 
                165                 170                 175     


Arg Ala Ser Ser Ser Val Ser Ser Ser Tyr Leu His Trp Tyr Gln Gln 
            180                 185                 190         


Lys Ser Gly Lys Ala Pro Lys Val Trp Ile Tyr Ser Thr Ser Asn Leu 
        195                 200                 205             


Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Tyr Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
225                 230                 235                 240 


Tyr Cys Gln Gln Tyr Ser Gly Tyr Pro Ile Thr Phe Gly Gln Gly Thr 
                245                 250                 255     


Lys Val Glu Ile Lys Arg Ser Asp Pro Thr Thr Thr Pro Ala Pro Arg 
            260                 265                 270         


Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg 
        275                 280                 285             


Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly 
    290                 295                 300                 


Leu Asp Phe Ala Cys Asp Ile Phe Trp Val Leu Val Val Val Gly Gly 
305                 310                 315                 320 


Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe 
                325                 330                 335     


Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn 
            340                 345                 350         


Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr 
        355                 360                 365             


Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Asp Gln Arg Leu 
    370                 375                 380                 


Pro Pro Asp Ala His Lys Pro Pro Gly Gly Gly Ser Phe Arg Thr Pro 
385                 390                 395                 400 


Ile Gln Glu Glu Gln Ala Asp Ala His Ser Thr Leu Ala Lys Ile Arg 
                405                 410                 415     


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln 
            420                 425                 430         


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
        435                 440                 445             


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
    450                 455                 460                 


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
465                 470                 475                 480 


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
                485                 490                 495     


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
            500                 505                 510         


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        515                 520                 525         


<210>  31
<211>  501
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-GD2 CAR, huKM666-HNG-CD28OXZ

<400>  31

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val 
            20                  25                  30          


Lys Pro Ser Gln Thr Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser 
        35                  40                  45              


Leu Ala Ser Tyr Asn Ile His Trp Val Arg Gln Pro Pro Gly Lys Gly 
    50                  55                  60                  


Leu Glu Trp Leu Gly Val Ile Trp Ala Gly Gly Ser Thr Asn Tyr Asn 
65                  70                  75                  80  


Ser Ala Leu Met Ser Arg Leu Thr Ile Ser Lys Asp Asn Ser Lys Asn 
                85                  90                  95      


Gln Val Phe Leu Lys Met Ser Ser Leu Thr Ala Ala Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Lys Arg Ser Asp Asp Tyr Ser Trp Phe Ala Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Asn Gln Met Thr Gln Ser 
145                 150                 155                 160 


Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Met Thr Cys 
                165                 170                 175     


Arg Ala Ser Ser Ser Val Ser Ser Ser Tyr Leu His Trp Tyr Gln Gln 
            180                 185                 190         


Lys Ser Gly Lys Ala Pro Lys Val Trp Ile Tyr Ser Thr Ser Asn Leu 
        195                 200                 205             


Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Tyr Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
225                 230                 235                 240 


Tyr Cys Gln Gln Tyr Ser Gly Tyr Pro Ile Thr Phe Gly Gln Gly Thr 
                245                 250                 255     


Lys Val Glu Ile Lys Arg Ser Asp Pro Ala Glu Pro Lys Ser Pro Asp 
            260                 265                 270         


Lys Thr His Thr Cys Pro Pro Cys Pro Lys Asp Pro Lys Phe Trp Val 
        275                 280                 285             


Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr 
    290                 295                 300                 


Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu 
305                 310                 315                 320 


His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg 
                325                 330                 335     


Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg 
            340                 345                 350         


Ser Arg Asp Gln Arg Leu Pro Pro Asp Ala His Lys Pro Pro Gly Gly 
        355                 360                 365             


Gly Ser Phe Arg Thr Pro Ile Gln Glu Glu Gln Ala Asp Ala His Ser 
    370                 375                 380                 


Thr Leu Ala Lys Ile Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro 
385                 390                 395                 400 


Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly 
                405                 410                 415     


Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro 
            420                 425                 430         


Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr 
        435                 440                 445             


Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly 
    450                 455                 460                 


Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln 
465                 470                 475                 480 


Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln 
                485                 490                 495     


Ala Leu Pro Pro Arg 
            500     


<210>  32
<211>  642
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-GD2 CAR, huKM666-HCH2CH3pvaa-CD28tmZ

<400>  32

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val 
            20                  25                  30          


Lys Pro Ser Gln Thr Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser 
        35                  40                  45              


Leu Ala Ser Tyr Asn Ile His Trp Val Arg Gln Pro Pro Gly Lys Gly 
    50                  55                  60                  


Leu Glu Trp Leu Gly Val Ile Trp Ala Gly Gly Ser Thr Asn Tyr Asn 
65                  70                  75                  80  


Ser Ala Leu Met Ser Arg Leu Thr Ile Ser Lys Asp Asn Ser Lys Asn 
                85                  90                  95      


Gln Val Phe Leu Lys Met Ser Ser Leu Thr Ala Ala Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Lys Arg Ser Asp Asp Tyr Ser Trp Phe Ala Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Asn Gln Met Thr Gln Ser 
145                 150                 155                 160 


Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Met Thr Cys 
                165                 170                 175     


Arg Ala Ser Ser Ser Val Ser Ser Ser Tyr Leu His Trp Tyr Gln Gln 
            180                 185                 190         


Lys Ser Gly Lys Ala Pro Lys Val Trp Ile Tyr Ser Thr Ser Asn Leu 
        195                 200                 205             


Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Tyr Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
225                 230                 235                 240 


Tyr Cys Gln Gln Tyr Ser Gly Tyr Pro Ile Thr Phe Gly Gln Gly Thr 
                245                 250                 255     


Lys Val Glu Ile Lys Arg Ser Asp Pro Ala Glu Pro Lys Ser Pro Asp 
            260                 265                 270         


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro 
        275                 280                 285             


Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ala 
    290                 295                 300                 


Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
305                 310                 315                 320 


Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
                325                 330                 335     


Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val 
            340                 345                 350         


Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
        355                 360                 365             


Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys 
    370                 375                 380                 


Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
385                 390                 395                 400 


Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr 
                405                 410                 415     


Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
            420                 425                 430         


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
        435                 440                 445             


Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys 
    450                 455                 460                 


Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
465                 470                 475                 480 


Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
                485                 490                 495     


Lys Lys Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val Leu 
            500                 505                 510         


Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
        515                 520                 525             


Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln 
    530                 535                 540                 


Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu 
545                 550                 555                 560 


Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly 
                565                 570                 575     


Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu 
            580                 585                 590         


Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly 
        595                 600                 605             


Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
    610                 615                 620                 


Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro 
625                 630                 635                 640 


Pro Arg 
        


<210>  33
<211>  681
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-GD2 CAR, huMK666-HCH2CH3pvaa-CD28Z

<400>  33

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu Val 
            20                  25                  30          


Lys Pro Ser Gln Thr Leu Ser Ile Thr Cys Thr Val Ser Gly Phe Ser 
        35                  40                  45              


Leu Ala Ser Tyr Asn Ile His Trp Val Arg Gln Pro Pro Gly Lys Gly 
    50                  55                  60                  


Leu Glu Trp Leu Gly Val Ile Trp Ala Gly Gly Ser Thr Asn Tyr Asn 
65                  70                  75                  80  


Ser Ala Leu Met Ser Arg Leu Thr Ile Ser Lys Asp Asn Ser Lys Asn 
                85                  90                  95      


Gln Val Phe Leu Lys Met Ser Ser Leu Thr Ala Ala Asp Thr Ala Val 
            100                 105                 110         


Tyr Tyr Cys Ala Lys Arg Ser Asp Asp Tyr Ser Trp Phe Ala Tyr Trp 
        115                 120                 125             


Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Asn Gln Met Thr Gln Ser 
145                 150                 155                 160 


Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Met Thr Cys 
                165                 170                 175     


Arg Ala Ser Ser Ser Val Ser Ser Ser Tyr Leu His Trp Tyr Gln Gln 
            180                 185                 190         


Lys Ser Gly Lys Ala Pro Lys Val Trp Ile Tyr Ser Thr Ser Asn Leu 
        195                 200                 205             


Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
    210                 215                 220                 


Tyr Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
225                 230                 235                 240 


Tyr Cys Gln Gln Tyr Ser Gly Tyr Pro Ile Thr Phe Gly Gln Gly Thr 
                245                 250                 255     


Lys Val Glu Ile Lys Arg Ser Asp Pro Ala Glu Pro Lys Ser Pro Asp 
            260                 265                 270         


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro 
        275                 280                 285             


Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ala 
    290                 295                 300                 


Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp 
305                 310                 315                 320 


Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn 
                325                 330                 335     


Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val 
            340                 345                 350         


Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu 
        355                 360                 365             


Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys 
    370                 375                 380                 


Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr 
385                 390                 395                 400 


Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr 
                405                 410                 415     


Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu 
            420                 425                 430         


Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu 
        435                 440                 445             


Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys 
    450                 455                 460                 


Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu 
465                 470                 475                 480 


Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly 
                485                 490                 495     


Lys Lys Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val Leu 
            500                 505                 510         


Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
        515                 520                 525             


Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
    530                 535                 540                 


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
545                 550                 555                 560 


Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg Ser 
                565                 570                 575     


Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu 
            580                 585                 590         


Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg 
        595                 600                 605             


Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln 
    610                 615                 620                 


Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr 
625                 630                 635                 640 


Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp 
                645                 650                 655     


Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala 
            660                 665                 670         


Leu His Met Gln Ala Leu Pro Pro Arg 
        675                 680     


<210>  34
<211>  1103
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-GD2 CAR co-expressed with iCasp9 suicide gene

<400>  34

Met Leu Glu Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg 
1               5                   10                  15      


Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met 
            20                  25                  30          


Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro 
        35                  40                  45              


Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu 
    50                  55                  60                  


Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser 
65                  70                  75                  80  


Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro 
                85                  90                  95      


His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Ser Gly 
            100                 105                 110         


Gly Gly Ser Gly Val Asp Gly Phe Gly Asp Val Gly Ala Leu Glu Ser 
        115                 120                 125             


Leu Arg Gly Asn Ala Asp Leu Ala Tyr Ile Leu Ser Met Glu Pro Cys 
    130                 135                 140                 


Gly His Cys Leu Ile Ile Asn Asn Val Asn Phe Cys Arg Glu Ser Gly 
145                 150                 155                 160 


Leu Arg Thr Arg Thr Gly Ser Asn Ile Asp Cys Glu Lys Leu Arg Arg 
                165                 170                 175     


Arg Phe Ser Ser Leu His Phe Met Val Glu Val Lys Gly Asp Leu Thr 
            180                 185                 190         


Ala Lys Lys Met Val Leu Ala Leu Leu Glu Leu Ala Gln Gln Asp His 
        195                 200                 205             


Gly Ala Leu Asp Cys Cys Val Val Val Ile Leu Ser His Gly Cys Gln 
    210                 215                 220                 


Ala Ser His Leu Gln Phe Pro Gly Ala Val Tyr Gly Thr Asp Gly Cys 
225                 230                 235                 240 


Pro Val Ser Val Glu Lys Ile Val Asn Ile Phe Asn Gly Thr Ser Cys 
                245                 250                 255     


Pro Ser Leu Gly Gly Lys Pro Lys Leu Phe Phe Ile Gln Ala Cys Gly 
            260                 265                 270         


Gly Glu Gln Lys Asp His Gly Phe Glu Val Ala Ser Thr Ser Pro Glu 
        275                 280                 285             


Asp Glu Ser Pro Gly Ser Asn Pro Glu Pro Asp Ala Thr Pro Phe Gln 
    290                 295                 300                 


Glu Gly Leu Arg Thr Phe Asp Gln Leu Asp Ala Ile Ser Ser Leu Pro 
305                 310                 315                 320 


Thr Pro Ser Asp Ile Phe Val Ser Tyr Ser Thr Phe Pro Gly Phe Val 
                325                 330                 335     


Ser Trp Arg Asp Pro Lys Ser Gly Ser Trp Tyr Val Glu Thr Leu Asp 
            340                 345                 350         


Asp Ile Phe Glu Gln Trp Ala His Ser Glu Asp Leu Gln Ser Leu Leu 
        355                 360                 365             


Leu Arg Val Ala Asn Ala Val Ser Val Lys Gly Ile Tyr Lys Gln Met 
    370                 375                 380                 


Pro Gly Cys Phe Asn Phe Leu Arg Lys Lys Leu Phe Phe Lys Thr Ser 
385                 390                 395                 400 


Ala Ser Arg Ala Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val 
                405                 410                 415     


Glu Glu Asn Pro Gly Pro Met Glu Thr Asp Thr Leu Leu Leu Trp Val 
            420                 425                 430         


Leu Leu Leu Trp Val Pro Gly Ser Thr Gly Gln Val Gln Leu Gln Glu 
        435                 440                 445             


Ser Gly Pro Gly Leu Val Lys Pro Ser Gln Thr Leu Ser Ile Thr Cys 
    450                 455                 460                 


Thr Val Ser Gly Phe Ser Leu Ala Ser Tyr Asn Ile His Trp Val Arg 
465                 470                 475                 480 


Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu Gly Val Ile Trp Ala Gly 
                485                 490                 495     


Gly Ser Thr Asn Tyr Asn Ser Ala Leu Met Ser Arg Leu Thr Ile Ser 
            500                 505                 510         


Lys Asp Asn Ser Lys Asn Gln Val Phe Leu Lys Met Ser Ser Leu Thr 
        515                 520                 525             


Ala Ala Asp Thr Ala Val Tyr Tyr Cys Ala Lys Arg Ser Asp Asp Tyr 
    530                 535                 540                 


Ser Trp Phe Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
545                 550                 555                 560 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
                565                 570                 575     


Asn Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp 
            580                 585                 590         


Arg Val Thr Met Thr Cys Arg Ala Ser Ser Ser Val Ser Ser Ser Tyr 
        595                 600                 605             


Leu His Trp Tyr Gln Gln Lys Ser Gly Lys Ala Pro Lys Val Trp Ile 
    610                 615                 620                 


Tyr Ser Thr Ser Asn Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly 
625                 630                 635                 640 


Ser Gly Ser Gly Thr Asp Tyr Thr Leu Thr Ile Ser Ser Leu Gln Pro 
                645                 650                 655     


Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Gly Tyr Pro Ile 
            660                 665                 670         


Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Ser Asp Pro Ala 
        675                 680                 685             


Glu Pro Lys Ser Pro Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
    690                 695                 700                 


Pro Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
705                 710                 715                 720 


Asp Thr Leu Met Ile Ala Arg Thr Pro Glu Val Thr Cys Val Val Val 
                725                 730                 735     


Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp 
            740                 745                 750         


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr 
        755                 760                 765             


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
    770                 775                 780                 


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu 
785                 790                 795                 800 


Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
                805                 810                 815     


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys 
            820                 825                 830         


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
        835                 840                 845             


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
    850                 855                 860                 


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
865                 870                 875                 880 


Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser 
                885                 890                 895     


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
            900                 905                 910         


Leu Ser Leu Ser Pro Gly Lys Lys Asp Pro Lys Phe Trp Val Leu Val 
        915                 920                 925             


Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala 
    930                 935                 940                 


Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser 
945                 950                 955                 960 


Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His 
                965                 970                 975     


Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg 
            980                 985                 990         


Val Lys Phe Ser Arg Ser Ala Asp  Ala Pro Ala Tyr Gln  Gln Gly Gln 
        995                 1000                 1005             


Asn Gln  Leu Tyr Asn Glu Leu  Asn Leu Gly Arg Arg  Glu Glu Tyr 
    1010                 1015                 1020             


Asp Val  Leu Asp Lys Arg Arg  Gly Arg Asp Pro Glu  Met Gly Gly 
    1025                 1030                 1035             


Lys Pro  Arg Arg Lys Asn Pro  Gln Glu Gly Leu Tyr  Asn Glu Leu 
    1040                 1045                 1050             


Gln Lys  Asp Lys Met Ala Glu  Ala Tyr Ser Glu Ile  Gly Met Lys 
    1055                 1060                 1065             


Gly Glu  Arg Arg Arg Gly Lys  Gly His Asp Gly Leu  Tyr Gln Gly 
    1070                 1075                 1080             


Leu Ser  Thr Ala Thr Lys Asp  Thr Tyr Asp Ala Leu  His Met Gln 
    1085                 1090                 1095             


Ala Leu  Pro Pro Arg 
    1100             


<210>  35
<211>  858
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-GD2 CAR co-expressed with RQR8 suicide gene

<400>  35

Met Gly Thr Ser Leu Leu Cys Trp Met Ala Leu Cys Leu Leu Gly Ala 
1               5                   10                  15      


Asp His Ala Asp Ala Cys Pro Tyr Ser Asn Pro Ser Leu Cys Ser Gly 
            20                  25                  30          


Gly Gly Gly Ser Glu Leu Pro Thr Gln Gly Thr Phe Ser Asn Val Ser 
        35                  40                  45              


Thr Asn Val Ser Pro Ala Lys Pro Thr Thr Thr Ala Cys Pro Tyr Ser 
    50                  55                  60                  


Asn Pro Ser Leu Cys Ser Gly Gly Gly Gly Ser Pro Ala Pro Arg Pro 
65                  70                  75                  80  


Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro 
                85                  90                  95      


Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu 
            100                 105                 110         


Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys 
        115                 120                 125             


Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg 
    130                 135                 140                 


Asn Arg Arg Arg Val Cys Lys Cys Pro Arg Pro Val Val Arg Ala Glu 
145                 150                 155                 160 


Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly 
                165                 170                 175     


Pro Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val 
            180                 185                 190         


Pro Gly Ser Thr Gly Gln Val Gln Leu Gln Glu Ser Gly Pro Gly Leu 
        195                 200                 205             


Val Lys Pro Ser Gln Thr Leu Ser Ile Thr Cys Thr Val Ser Gly Phe 
    210                 215                 220                 


Ser Leu Ala Ser Tyr Asn Ile His Trp Val Arg Gln Pro Pro Gly Lys 
225                 230                 235                 240 


Gly Leu Glu Trp Leu Gly Val Ile Trp Ala Gly Gly Ser Thr Asn Tyr 
                245                 250                 255     


Asn Ser Ala Leu Met Ser Arg Leu Thr Ile Ser Lys Asp Asn Ser Lys 
            260                 265                 270         


Asn Gln Val Phe Leu Lys Met Ser Ser Leu Thr Ala Ala Asp Thr Ala 
        275                 280                 285             


Val Tyr Tyr Cys Ala Lys Arg Ser Asp Asp Tyr Ser Trp Phe Ala Tyr 
    290                 295                 300                 


Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Gly Gly Gly Gly Ser 
305                 310                 315                 320 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Asn Gln Met Thr Gln 
                325                 330                 335     


Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Met Thr 
            340                 345                 350         


Cys Arg Ala Ser Ser Ser Val Ser Ser Ser Tyr Leu His Trp Tyr Gln 
        355                 360                 365             


Gln Lys Ser Gly Lys Ala Pro Lys Val Trp Ile Tyr Ser Thr Ser Asn 
    370                 375                 380                 


Leu Ala Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr 
385                 390                 395                 400 


Asp Tyr Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr 
                405                 410                 415     


Tyr Tyr Cys Gln Gln Tyr Ser Gly Tyr Pro Ile Thr Phe Gly Gln Gly 
            420                 425                 430         


Thr Lys Val Glu Ile Lys Arg Ser Asp Pro Ala Glu Pro Lys Ser Pro 
        435                 440                 445             


Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly 
    450                 455                 460                 


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
465                 470                 475                 480 


Ala Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
                485                 490                 495     


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
            500                 505                 510         


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 
        515                 520                 525             


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
    530                 535                 540                 


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
545                 550                 555                 560 


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
                565                 570                 575     


Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu 
            580                 585                 590         


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
        595                 600                 605             


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
    610                 615                 620                 


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
625                 630                 635                 640 


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
                645                 650                 655     


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
            660                 665                 670         


Gly Lys Lys Asp Pro Lys Phe Trp Val Leu Val Val Val Gly Gly Val 
        675                 680                 685             


Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp 
    690                 695                 700                 


Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met 
705                 710                 715                 720 


Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala 
                725                 730                 735     


Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val Lys Phe Ser Arg 
            740                 745                 750         


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
        755                 760                 765             


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
    770                 775                 780                 


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
785                 790                 795                 800 


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
                805                 810                 815     


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
            820                 825                 830         


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
        835                 840                 845             


Ala Leu His Met Gln Ala Leu Pro Pro Arg 
    850                 855             


<210>  36
<211>  402
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Inducible Caspase 9 (iCasp9) sequence

<400>  36

Met Leu Glu Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg 
1               5                   10                  15      


Thr Phe Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met 
            20                  25                  30          


Leu Glu Asp Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro 
        35                  40                  45              


Phe Lys Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu 
    50                  55                  60                  


Gly Val Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser 
65                  70                  75                  80  


Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro 
                85                  90                  95      


His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu Ser Gly 
            100                 105                 110         


Gly Gly Ser Gly Val Asp Gly Phe Gly Asp Val Gly Ala Leu Glu Ser 
        115                 120                 125             


Leu Arg Gly Asn Ala Asp Leu Ala Tyr Ile Leu Ser Met Glu Pro Cys 
    130                 135                 140                 


Gly His Cys Leu Ile Ile Asn Asn Val Asn Phe Cys Arg Glu Ser Gly 
145                 150                 155                 160 


Leu Arg Thr Arg Thr Gly Ser Asn Ile Asp Cys Glu Lys Leu Arg Arg 
                165                 170                 175     


Arg Phe Ser Ser Leu His Phe Met Val Glu Val Lys Gly Asp Leu Thr 
            180                 185                 190         


Ala Lys Lys Met Val Leu Ala Leu Leu Glu Leu Ala Gln Gln Asp His 
        195                 200                 205             


Gly Ala Leu Asp Cys Cys Val Val Val Ile Leu Ser His Gly Cys Gln 
    210                 215                 220                 


Ala Ser His Leu Gln Phe Pro Gly Ala Val Tyr Gly Thr Asp Gly Cys 
225                 230                 235                 240 


Pro Val Ser Val Glu Lys Ile Val Asn Ile Phe Asn Gly Thr Ser Cys 
                245                 250                 255     


Pro Ser Leu Gly Gly Lys Pro Lys Leu Phe Phe Ile Gln Ala Cys Gly 
            260                 265                 270         


Gly Glu Gln Lys Asp His Gly Phe Glu Val Ala Ser Thr Ser Pro Glu 
        275                 280                 285             


Asp Glu Ser Pro Gly Ser Asn Pro Glu Pro Asp Ala Thr Pro Phe Gln 
    290                 295                 300                 


Glu Gly Leu Arg Thr Phe Asp Gln Leu Asp Ala Ile Ser Ser Leu Pro 
305                 310                 315                 320 


Thr Pro Ser Asp Ile Phe Val Ser Tyr Ser Thr Phe Pro Gly Phe Val 
                325                 330                 335     


Ser Trp Arg Asp Pro Lys Ser Gly Ser Trp Tyr Val Glu Thr Leu Asp 
            340                 345                 350         


Asp Ile Phe Glu Gln Trp Ala His Ser Glu Asp Leu Gln Ser Leu Leu 
        355                 360                 365             


Leu Arg Val Ala Asn Ala Val Ser Val Lys Gly Ile Tyr Lys Gln Met 
    370                 375                 380                 


Pro Gly Cys Phe Asn Phe Leu Arg Lys Lys Leu Phe Phe Lys Thr Ser 
385                 390                 395                 400 


Ala Ser 
        


<210>  37
<211>  157
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Novel marker/suicide gene RQR8 sequence

<400>  37

Met Gly Thr Ser Leu Leu Cys Trp Met Ala Leu Cys Leu Leu Gly Ala 
1               5                   10                  15      


Asp His Ala Asp Ala Cys Pro Tyr Ser Asn Pro Ser Leu Cys Ser Gly 
            20                  25                  30          


Gly Gly Gly Ser Glu Leu Pro Thr Gln Gly Thr Phe Ser Asn Val Ser 
        35                  40                  45              


Thr Asn Val Ser Pro Ala Lys Pro Thr Thr Thr Ala Cys Pro Tyr Ser 
    50                  55                  60                  


Asn Pro Ser Leu Cys Ser Gly Gly Gly Gly Ser Pro Ala Pro Arg Pro 
65                  70                  75                  80  


Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro 
                85                  90                  95      


Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu 
            100                 105                 110         


Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys 
        115                 120                 125             


Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His Arg 
    130                 135                 140                 


Asn Arg Arg Arg Val Cys Lys Cys Pro Arg Pro Val Val 
145                 150                 155         


