                         SEQUENCE LISTING

<110>  National Research Council of Canada
       Stanimirovic, Danica
       Kemmerich, Kristin
       Sulea, Traian
       Arbabi-Ghahroudi, Mehdi
       Haqqani, Arsalan
       Gilbert, Renald
       Massie, Bernard
 
<120>  Insulin-Like Growth Factor 1 Receptor-Specific Antibodies and 
       Uses Thereof

<130>  12600-98

<150>  61/948,831
<151>  2014-03-06

<160>  42    

<170>  PatentIn version 3.5

<210>  1
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  IGF1R-5 CDR1

<400>  1

Gly Arg Thr Ile Asp Asn Tyr Ala 
1               5               


<210>  2
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  IGF1R-5 CDR2


<220>
<221>  MISC_FEATURE
<222>  (8)..(8)
<223>  Xaa is Ala or Thr

<400>  2

Ile Asp Trp Gly Asp Gly Gly Xaa 
1               5               


<210>  3
<211>  17
<212>  PRT
<213>  Artificial

<220>
<223>  IGF1R-5 CDR3

<400>  3

Ala Met Ala Arg Gln Ser Arg Val Asn Leu Asp Val Ala Arg Tyr Asp 
1               5                   10                  15      


Tyr 
    


<210>  4
<211>  124
<212>  PRT
<213>  Artificial

<220>
<223>  IGF1R-5 Consensus


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is Glu or Gln

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is Lys or Gln

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is Val or Glu

<220>
<221>  MISC_FEATURE
<222>  (14)..(14)
<223>  Xaa is Ala or Pro

<220>
<221>  MISC_FEATURE
<222>  (37)..(37)
<223>  Xaa is Val or Ser

<220>
<221>  MISC_FEATURE
<222>  (44)..(44)
<223>  Xaa is Asp or Gly

<220>
<221>  MISC_FEATURE
<222>  (45)..(45)
<223>  Xaa is Leu or Arg

<220>
<221>  MISC_FEATURE
<222>  (47)..(47)
<223>  Xaa is Phe or Trp

<220>
<221>  MISC_FEATURE
<222>  (49)..(49)
<223>  Xaa is Ala or Ser

<220>
<221>  MISC_FEATURE
<222>  (58)..(58)
<223>  Xaa is Ala or Thr

<220>
<221>  MISC_FEATURE
<222>  (75)..(75)
<223>  Xaa is Ala or Ser

<220>
<221>  MISC_FEATURE
<222>  (77)..(77)
<223>  Xaa is Gly or Asn

<220>
<221>  MISC_FEATURE
<222>  (79)..(79)
<223>  Xaa is Met or Leu

<220>
<221>  MISC_FEATURE
<222>  (85)..(85)
<223>  Xaa is Asn or Arg

<220>
<221>  MISC_FEATURE
<222>  (87)..(87)
<223>  Xaa is Glu or Arg

<220>
<221>  MISC_FEATURE
<222>  (88)..(88)
<223>  Xaa is Pro or Ala

<220>
<221>  MISC_FEATURE
<222>  (95)..(95)
<223>  Xaa is Ser or Tyr

<220>
<221>  MISC_FEATURE
<222>  (119)..(119)
<223>  Xaa is Gln or Leu

<400>  4

Xaa Val Xaa Leu Xaa Glu Ser Gly Gly Gly Leu Val Gln Xaa Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Ile Asp Asn Tyr 
            20                  25                  30          


Ala Met Ala Trp Xaa Arg Gln Ala Pro Gly Lys Xaa Xaa Glu Xaa Val 
        35                  40                  45              


Xaa Thr Ile Asp Trp Gly Asp Gly Gly Xaa Arg Tyr Ala Asn Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Xaa Lys Xaa Thr Xaa Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Xaa Leu Xaa Xaa Glu Asp Thr Ala Val Tyr Xaa Cys 
                85                  90                  95      


Ala Met Ala Arg Gln Ser Arg Val Asn Leu Asp Val Ala Arg Tyr Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Xaa Val Thr Val Ser Ser 
        115                 120                 


<210>  5
<211>  124
<212>  PRT
<213>  Artificial

<220>
<223>  IGF1R-5 VHH

<400>  5

Gln Val Lys Leu Glu Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Ile Asp Asn Tyr 
            20                  25                  30          


Ala Met Ala Trp Ser Arg Gln Ala Pro Gly Lys Asp Arg Glu Phe Val 
        35                  40                  45              


Ala Thr Ile Asp Trp Gly Asp Gly Gly Ala Arg Tyr Ala Asn Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Gly Thr Met Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Asn Leu Glu Pro Glu Asp Thr Ala Val Tyr Ser Cys 
                85                  90                  95      


Ala Met Ala Arg Gln Ser Arg Val Asn Leu Asp Val Ala Arg Tyr Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 


<210>  6
<211>  124
<212>  PRT
<213>  Artificial

<220>
<223>  IGF1R-5_H1

<400>  6

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Ile Asp Asn Tyr 
            20                  25                  30          


Ala Met Ala Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Thr Ile Asp Trp Gly Asp Gly Gly Thr Arg Tyr Ala Asn Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Met Ala Arg Gln Ser Arg Val Asn Leu Asp Val Ala Arg Tyr Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120                 


<210>  7
<211>  124
<212>  PRT
<213>  Artificial

<220>
<223>  IGF1R-5_H2

<400>  7

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Ile Asp Asn Tyr 
            20                  25                  30          


Ala Met Ala Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Thr Ile Asp Trp Gly Asp Gly Gly Thr Arg Tyr Ala Asn Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Met Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Met Ala Arg Gln Ser Arg Val Asn Leu Asp Val Ala Arg Tyr Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120                 


<210>  8
<211>  124
<212>  PRT
<213>  Artificial

<220>
<223>  IGF1R-5_H3

<400>  8

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Ile Asp Asn Tyr 
            20                  25                  30          


Ala Met Ala Trp Ser Arg Gln Ala Pro Gly Lys Gly Leu Glu Phe Val 
        35                  40                  45              


Ala Thr Ile Asp Trp Gly Asp Gly Gly Thr Arg Tyr Ala Asn Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Met Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Met Ala Arg Gln Ser Arg Val Asn Leu Asp Val Ala Arg Tyr Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120                 


<210>  9
<211>  124
<212>  PRT
<213>  Artificial

<220>
<223>  IGF1R-5_H5

<400>  9

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Ile Asp Asn Tyr 
            20                  25                  30          


Ala Met Ala Trp Ser Arg Gln Ala Pro Gly Lys Asp Arg Glu Phe Val 
        35                  40                  45              


Ala Thr Ile Asp Trp Gly Asp Gly Gly Thr Arg Tyr Ala Asn Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Gly Thr Met Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Ser Cys 
                85                  90                  95      


Ala Met Ala Arg Gln Ser Arg Val Asn Leu Asp Val Ala Arg Tyr Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120                 


<210>  10
<211>  124
<212>  PRT
<213>  Artificial

<220>
<223>  IGF1R-5_H6

<400>  10

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Ile Asp Asn Tyr 
            20                  25                  30          


Ala Met Ala Trp Ser Arg Gln Ala Pro Gly Lys Asp Arg Glu Phe Val 
        35                  40                  45              


Ser Thr Ile Asp Trp Gly Asp Gly Gly Thr Arg Tyr Ala Asn Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Met Ala Arg Gln Ser Arg Val Asn Leu Asp Val Ala Arg Tyr Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120                 


<210>  11
<211>  13
<212>  PRT
<213>  Artificial

<220>
<223>  IGF1R-5 epitope

<400>  11

Phe Glu Asn Phe Leu His Asn Ser Ile Phe Val Pro Arg 
1               5                   10              


<210>  12
<211>  370
<212>  PRT
<213>  Artificial

<220>
<223>  IGF1R-5-mFc fusion

<400>  12

Gln Val Lys Leu Glu Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Ile Asp Asn Tyr 
            20                  25                  30          


Ala Met Ala Trp Ser Arg Gln Ala Pro Gly Lys Asp Arg Glu Phe Val 
        35                  40                  45              


Ala Thr Ile Asp Trp Gly Asp Gly Gly Ala Arg Tyr Ala Asn Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Gly Thr Met Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Asn Leu Glu Pro Glu Asp Thr Ala Val Tyr Ser Cys 
                85                  90                  95      


Ala Met Ala Arg Gln Ser Arg Val Asn Leu Asp Val Ala Arg Tyr Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser Met Thr Val Asp 
        115                 120                 125             


Lys Lys Leu Glu Pro Ser Gly Pro Ile Ser Thr Ile Asn Pro Cys Pro 
    130                 135                 140                 


Pro Cys Lys Glu Cys His Lys Cys Pro Ala Pro Asn Leu Glu Gly Gly 
145                 150                 155                 160 


Pro Ser Val Phe Ile Phe Pro Pro Asn Ile Lys Asp Val Leu Met Ile 
                165                 170                 175     


Ser Leu Thr Pro Lys Val Thr Cys Val Val Val Asp Val Ser Glu Asp 
            180                 185                 190         


Asp Pro Asp Val Gln Ile Ser Trp Phe Val Asn Asn Val Glu Val His 
        195                 200                 205             


Thr Ala Gln Thr Gln Thr His Arg Glu Asp Tyr Asn Ser Thr Ile Arg 
    210                 215                 220                 


Val Val Ser Thr Leu Pro Ile Gln His Gln Asp Trp Met Ser Gly Lys 
225                 230                 235                 240 


Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu Pro Ser Pro Ile Glu 
                245                 250                 255     


Arg Thr Ile Ser Lys Ile Lys Gly Leu Val Arg Ala Pro Gln Val Tyr 
            260                 265                 270         


Ile Leu Pro Pro Pro Ala Glu Gln Leu Ser Arg Lys Asp Val Ser Leu 
        275                 280                 285             


Thr Cys Leu Val Val Gly Phe Asn Pro Gly Asp Ile Ser Val Glu Trp 
    290                 295                 300                 


Thr Ser Asn Gly His Thr Glu Glu Asn Tyr Lys Asp Thr Ala Pro Val 
305                 310                 315                 320 


Leu Asp Ser Asp Gly Ser Tyr Phe Ile Tyr Ser Lys Leu Asn Met Lys 
                325                 330                 335     


Thr Ser Lys Trp Glu Lys Thr Asp Ser Phe Ser Cys Asn Val Arg His 
            340                 345                 350         


Glu Gly Leu Lys Asn Tyr Tyr Leu Lys Lys Thr Ile Ser Arg Ser Pro 
        355                 360                 365             


Gly Lys 
    370 


<210>  13
<211>  371
<212>  PRT
<213>  Artificial

<220>
<223>  mFc-IGF1R-5 fusion

<400>  13

Glu Pro Ser Gly Pro Ile Ser Thr Ile Asn Pro Cys Pro Pro Cys Lys 
1               5                   10                  15      


Glu Cys His Lys Cys Pro Ala Pro Asn Leu Glu Gly Gly Pro Ser Val 
            20                  25                  30          


Phe Ile Phe Pro Pro Asn Ile Lys Asp Val Leu Met Ile Ser Leu Thr 
        35                  40                  45              


Pro Lys Val Thr Cys Val Val Val Asp Val Ser Glu Asp Asp Pro Asp 
    50                  55                  60                  


Val Gln Ile Ser Trp Phe Val Asn Asn Val Glu Val His Thr Ala Gln 
65                  70                  75                  80  


Thr Gln Thr His Arg Glu Asp Tyr Asn Ser Thr Ile Arg Val Val Ser 
                85                  90                  95      


Thr Leu Pro Ile Gln His Gln Asp Trp Met Ser Gly Lys Glu Phe Lys 
            100                 105                 110         


Cys Lys Val Asn Asn Lys Asp Leu Pro Ser Pro Ile Glu Arg Thr Ile 
        115                 120                 125             


Ser Lys Ile Lys Gly Leu Val Arg Ala Pro Gln Val Tyr Ile Leu Pro 
    130                 135                 140                 


Pro Pro Ala Glu Gln Leu Ser Arg Lys Asp Val Ser Leu Thr Cys Leu 
145                 150                 155                 160 


Val Val Gly Phe Asn Pro Gly Asp Ile Ser Val Glu Trp Thr Ser Asn 
                165                 170                 175     


Gly His Thr Glu Glu Asn Tyr Lys Asp Thr Ala Pro Val Leu Asp Ser 
            180                 185                 190         


Asp Gly Ser Tyr Phe Ile Tyr Ser Lys Leu Asn Met Lys Thr Ser Lys 
        195                 200                 205             


Trp Glu Lys Thr Asp Ser Phe Ser Cys Asn Val Arg His Glu Gly Leu 
    210                 215                 220                 


Lys Asn Tyr Tyr Leu Lys Lys Thr Ile Ser Arg Ser Pro Gly Gly Gly 
225                 230                 235                 240 


Gly Ser Gly Gly Gly Gly Ser Gln Val Lys Leu Glu Glu Ser Gly Gly 
                245                 250                 255     


Gly Leu Val Gln Ala Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser 
            260                 265                 270         


Gly Arg Thr Ile Asp Asn Tyr Ala Met Ala Trp Ser Arg Gln Ala Pro 
        275                 280                 285             


Gly Lys Asp Arg Glu Phe Val Ala Thr Ile Asp Trp Gly Asp Gly Gly 
    290                 295                 300                 


Ala Arg Tyr Ala Asn Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp 
305                 310                 315                 320 


Asn Ala Lys Gly Thr Met Tyr Leu Gln Met Asn Asn Leu Glu Pro Glu 
                325                 330                 335     


Asp Thr Ala Val Tyr Ser Cys Ala Met Ala Arg Gln Ser Arg Val Asn 
            340                 345                 350         


Leu Asp Val Ala Arg Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr 
        355                 360                 365             


Val Ser Ser 
    370     


<210>  14
<211>  1367
<212>  PRT
<213>  Artificial

<220>
<223>  Human IGF1R

<400>  14

Met Lys Ser Gly Ser Gly Gly Gly Ser Pro Thr Ser Leu Trp Gly Leu 
1               5                   10                  15      


Leu Phe Leu Ser Ala Ala Leu Ser Leu Trp Pro Thr Ser Gly Glu Ile 
            20                  25                  30          


Cys Gly Pro Gly Ile Asp Ile Arg Asn Asp Tyr Gln Gln Leu Lys Arg 
        35                  40                  45              


Leu Glu Asn Cys Thr Val Ile Glu Gly Tyr Leu His Ile Leu Leu Ile 
    50                  55                  60                  


Ser Lys Ala Glu Asp Tyr Arg Ser Tyr Arg Phe Pro Lys Leu Thr Val 
65                  70                  75                  80  


Ile Thr Glu Tyr Leu Leu Leu Phe Arg Val Ala Gly Leu Glu Ser Leu 
                85                  90                  95      


Gly Asp Leu Phe Pro Asn Leu Thr Val Ile Arg Gly Trp Lys Leu Phe 
            100                 105                 110         


Tyr Asn Tyr Ala Leu Val Ile Phe Glu Met Thr Asn Leu Lys Asp Ile 
        115                 120                 125             


Gly Leu Tyr Asn Leu Arg Asn Ile Thr Arg Gly Ala Ile Arg Ile Glu 
    130                 135                 140                 


Lys Asn Ala Asp Leu Cys Tyr Leu Ser Thr Val Asp Trp Ser Leu Ile 
145                 150                 155                 160 


Leu Asp Ala Val Ser Asn Asn Tyr Ile Val Gly Asn Lys Pro Pro Lys 
                165                 170                 175     


Glu Cys Gly Asp Leu Cys Pro Gly Thr Met Glu Glu Lys Pro Met Cys 
            180                 185                 190         


Glu Lys Thr Thr Ile Asn Asn Glu Tyr Asn Tyr Arg Cys Trp Thr Thr 
        195                 200                 205             


Asn Arg Cys Gln Lys Met Cys Pro Ser Thr Cys Gly Lys Arg Ala Cys 
    210                 215                 220                 


Thr Glu Asn Asn Glu Cys Cys His Pro Glu Cys Leu Gly Ser Cys Ser 
225                 230                 235                 240 


Ala Pro Asp Asn Asp Thr Ala Cys Val Ala Cys Arg His Tyr Tyr Tyr 
                245                 250                 255     


Ala Gly Val Cys Val Pro Ala Cys Pro Pro Asn Thr Tyr Arg Phe Glu 
            260                 265                 270         


Gly Trp Arg Cys Val Asp Arg Asp Phe Cys Ala Asn Ile Leu Ser Ala 
        275                 280                 285             


Glu Ser Ser Asp Ser Glu Gly Phe Val Ile His Asp Gly Glu Cys Met 
    290                 295                 300                 


Gln Glu Cys Pro Ser Gly Phe Ile Arg Asn Gly Ser Gln Ser Met Tyr 
305                 310                 315                 320 


Cys Ile Pro Cys Glu Gly Pro Cys Pro Lys Val Cys Glu Glu Glu Lys 
                325                 330                 335     


Lys Thr Lys Thr Ile Asp Ser Val Thr Ser Ala Gln Met Leu Gln Gly 
            340                 345                 350         


Cys Thr Ile Phe Lys Gly Asn Leu Leu Ile Asn Ile Arg Arg Gly Asn 
        355                 360                 365             


Asn Ile Ala Ser Glu Leu Glu Asn Phe Met Gly Leu Ile Glu Val Val 
    370                 375                 380                 


Thr Gly Tyr Val Lys Ile Arg His Ser His Ala Leu Val Ser Leu Ser 
385                 390                 395                 400 


Phe Leu Lys Asn Leu Arg Leu Ile Leu Gly Glu Glu Gln Leu Glu Gly 
                405                 410                 415     


Asn Tyr Ser Phe Tyr Val Leu Asp Asn Gln Asn Leu Gln Gln Leu Trp 
            420                 425                 430         


Asp Trp Asp His Arg Asn Leu Thr Ile Lys Ala Gly Lys Met Tyr Phe 
        435                 440                 445             


Ala Phe Asn Pro Lys Leu Cys Val Ser Glu Ile Tyr Arg Met Glu Glu 
    450                 455                 460                 


Val Thr Gly Thr Lys Gly Arg Gln Ser Lys Gly Asp Ile Asn Thr Arg 
465                 470                 475                 480 


Asn Asn Gly Glu Arg Ala Ser Cys Glu Ser Asp Val Leu His Phe Thr 
                485                 490                 495     


Ser Thr Thr Thr Ser Lys Asn Arg Ile Ile Ile Thr Trp His Arg Tyr 
            500                 505                 510         


Arg Pro Pro Asp Tyr Arg Asp Leu Ile Ser Phe Thr Val Tyr Tyr Lys 
        515                 520                 525             


Glu Ala Pro Phe Lys Asn Val Thr Glu Tyr Asp Gly Gln Asp Ala Cys 
    530                 535                 540                 


Gly Ser Asn Ser Trp Asn Met Val Asp Val Asp Leu Pro Pro Asn Lys 
545                 550                 555                 560 


Asp Val Glu Pro Gly Ile Leu Leu His Gly Leu Lys Pro Trp Thr Gln 
                565                 570                 575     


Tyr Ala Val Tyr Val Lys Ala Val Thr Leu Thr Met Val Glu Asn Asp 
            580                 585                 590         


His Ile Arg Gly Ala Lys Ser Glu Ile Leu Tyr Ile Arg Thr Asn Ala 
        595                 600                 605             


Ser Val Pro Ser Ile Pro Leu Asp Val Leu Ser Ala Ser Asn Ser Ser 
    610                 615                 620                 


Ser Gln Leu Ile Val Lys Trp Asn Pro Pro Ser Leu Pro Asn Gly Asn 
625                 630                 635                 640 


Leu Ser Tyr Tyr Ile Val Arg Trp Gln Arg Gln Pro Gln Asp Gly Tyr 
                645                 650                 655     


Leu Tyr Arg His Asn Tyr Cys Ser Lys Asp Lys Ile Pro Ile Arg Lys 
            660                 665                 670         


Tyr Ala Asp Gly Thr Ile Asp Ile Glu Glu Val Thr Glu Asn Pro Lys 
        675                 680                 685             


Thr Glu Val Cys Gly Gly Glu Lys Gly Pro Cys Cys Ala Cys Pro Lys 
    690                 695                 700                 


Thr Glu Ala Glu Lys Gln Ala Glu Lys Glu Glu Ala Glu Tyr Arg Lys 
705                 710                 715                 720 


Val Phe Glu Asn Phe Leu His Asn Ser Ile Phe Val Pro Arg Pro Glu 
                725                 730                 735     


Arg Lys Arg Arg Asp Val Met Gln Val Ala Asn Thr Thr Met Ser Ser 
            740                 745                 750         


Arg Ser Arg Asn Thr Thr Ala Ala Asp Thr Tyr Asn Ile Thr Asp Pro 
        755                 760                 765             


Glu Glu Leu Glu Thr Glu Tyr Pro Phe Phe Glu Ser Arg Val Asp Asn 
    770                 775                 780                 


Lys Glu Arg Thr Val Ile Ser Asn Leu Arg Pro Phe Thr Leu Tyr Arg 
785                 790                 795                 800 


Ile Asp Ile His Ser Cys Asn His Glu Ala Glu Lys Leu Gly Cys Ser 
                805                 810                 815     


Ala Ser Asn Phe Val Phe Ala Arg Thr Met Pro Ala Glu Gly Ala Asp 
            820                 825                 830         


Asp Ile Pro Gly Pro Val Thr Trp Glu Pro Arg Pro Glu Asn Ser Ile 
        835                 840                 845             


Phe Leu Lys Trp Pro Glu Pro Glu Asn Pro Asn Gly Leu Ile Leu Met 
    850                 855                 860                 


Tyr Glu Ile Lys Tyr Gly Ser Gln Val Glu Asp Gln Arg Glu Cys Val 
865                 870                 875                 880 


Ser Arg Gln Glu Tyr Arg Lys Tyr Gly Gly Ala Lys Leu Asn Arg Leu 
                885                 890                 895     


Asn Pro Gly Asn Tyr Thr Ala Arg Ile Gln Ala Thr Ser Leu Ser Gly 
            900                 905                 910         


Asn Gly Ser Trp Thr Asp Pro Val Phe Phe Tyr Val Gln Ala Lys Thr 
        915                 920                 925             


Gly Tyr Glu Asn Phe Ile His Leu Ile Ile Ala Leu Pro Val Ala Val 
    930                 935                 940                 


Leu Leu Ile Val Gly Gly Leu Val Ile Met Leu Tyr Val Phe His Arg 
945                 950                 955                 960 


Lys Arg Asn Asn Ser Arg Leu Gly Asn Gly Val Leu Tyr Ala Ser Val 
                965                 970                 975     


Asn Pro Glu Tyr Phe Ser Ala Ala Asp Val Tyr Val Pro Asp Glu Trp 
            980                 985                 990         


Glu Val Ala Arg Glu Lys Ile Thr  Met Ser Arg Glu Leu  Gly Gln Gly 
        995                 1000                 1005             


Ser Phe  Gly Met Val Tyr Glu  Gly Val Ala Lys Gly  Val Val Lys 
    1010                 1015                 1020             


Asp Glu  Pro Glu Thr Arg Val  Ala Ile Lys Thr Val  Asn Glu Ala 
    1025                 1030                 1035             


Ala Ser  Met Arg Glu Arg Ile  Glu Phe Leu Asn Glu  Ala Ser Val 
    1040                 1045                 1050             


Met Lys  Glu Phe Asn Cys His  His Val Val Arg Leu  Leu Gly Val 
    1055                 1060                 1065             


Val Ser  Gln Gly Gln Pro Thr  Leu Val Ile Met Glu  Leu Met Thr 
    1070                 1075                 1080             


Arg Gly  Asp Leu Lys Ser Tyr  Leu Arg Ser Leu Arg  Pro Glu Met 
    1085                 1090                 1095             


Glu Asn  Asn Pro Val Leu Ala  Pro Pro Ser Leu Ser  Lys Met Ile 
    1100                 1105                 1110             


Gln Met  Ala Gly Glu Ile Ala  Asp Gly Met Ala Tyr  Leu Asn Ala 
    1115                 1120                 1125             


Asn Lys  Phe Val His Arg Asp  Leu Ala Ala Arg Asn  Cys Met Val 
    1130                 1135                 1140             


Ala Glu  Asp Phe Thr Val Lys  Ile Gly Asp Phe Gly  Met Thr Arg 
    1145                 1150                 1155             


Asp Ile  Tyr Glu Thr Asp Tyr  Tyr Arg Lys Gly Gly  Lys Gly Leu 
    1160                 1165                 1170             


Leu Pro  Val Arg Trp Met Ser  Pro Glu Ser Leu Lys  Asp Gly Val 
    1175                 1180                 1185             


Phe Thr  Thr Tyr Ser Asp Val  Trp Ser Phe Gly Val  Val Leu Trp 
    1190                 1195                 1200             


Glu Ile  Ala Thr Leu Ala Glu  Gln Pro Tyr Gln Gly  Leu Ser Asn 
    1205                 1210                 1215             


Glu Gln  Val Leu Arg Phe Val  Met Glu Gly Gly Leu  Leu Asp Lys 
    1220                 1225                 1230             


Pro Asp  Asn Cys Pro Asp Met  Leu Phe Glu Leu Met  Arg Met Cys 
    1235                 1240                 1245             


Trp Gln  Tyr Asn Pro Lys Met  Arg Pro Ser Phe Leu  Glu Ile Ile 
    1250                 1255                 1260             


Ser Ser  Ile Lys Glu Glu Met  Glu Pro Gly Phe Arg  Glu Val Ser 
    1265                 1270                 1275             


Phe Tyr  Tyr Ser Glu Glu Asn  Lys Leu Pro Glu Pro  Glu Glu Leu 
    1280                 1285                 1290             


Asp Leu  Glu Pro Glu Asn Met  Glu Ser Val Pro Leu  Asp Pro Ser 
    1295                 1300                 1305             


Ala Ser  Ser Ser Ser Leu Pro  Leu Pro Asp Arg His  Ser Gly His 
    1310                 1315                 1320             


Lys Ala  Glu Asn Gly Pro Gly  Pro Gly Val Leu Val  Leu Arg Ala 
    1325                 1330                 1335             


Ser Phe  Asp Glu Arg Gln Pro  Tyr Ala His Met Asn  Gly Gly Arg 
    1340                 1345                 1350             


Lys Asn  Glu Arg Ala Leu Pro  Leu Pro Gln Ser Ser  Thr Cys 
    1355                 1360                 1365         


<210>  15
<211>  4
<212>  PRT
<213>  Artificial

<220>
<223>  Furin cleavage site

<400>  15

Arg Lys Arg Arg 
1               


<210>  16
<211>  33
<212>  DNA
<213>  Artificial

<220>
<223>  Forward primer

<400>  16
cgggatccgc caccatgaag tctggctccg gag                                    33


<210>  17
<211>  34
<212>  DNA
<213>  Artificial

<220>
<223>  Reverse primer

<400>  17
gctctagatc agaagttttc atatcctgtt ttgg                                   34


<210>  18
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  MJ1 primer

<400>  18
gcccagccgg ccatggccsm kgtgcagctg gtggaktctg gggga                       45


<210>  19
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  MJ2 primer

<400>  19
gcccagccgg ccatggccca ggtaaagctg gaggagtctg gggga                       45


<210>  20
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  MJ3 primer

<400>  20
gcccagccgg ccatggccca ggctcaggta cagctggtgg agtct                       45


<210>  21
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  CH2 primer

<400>  21
cgccatcaag gtaccagttg a                                                 21


<210>  22
<211>  29
<212>  DNA
<213>  Artificial

<220>
<223>  CH2b3 primer

<400>  22
ggggtacctg tcatccacgg accagctga                                         29


<210>  23
<211>  34
<212>  DNA
<213>  Artificial

<220>
<223>  MJ7 primer

<400>  23
catgtgtaga ctcgcggccc agccggccat ggcc                                   34


<210>  24
<211>  47
<212>  DNA
<213>  Artificial

<220>
<223>  MJ8 primer

<400>  24
catgtgtaga ttcctggccg gcctggcctg aggagacggt gacctgg                     47


<210>  25
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  Forward primer

<400>  25
tatgaagaca ccaggcccag gtaaagctgg aggagtct                               38


<210>  26
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  Reverse primer

<400>  26
ttgttcggat cctgaggaga cggtgacctg                                        30


<210>  27
<211>  14
<212>  PRT
<213>  Artificial

<220>
<223>  IGF1R-5 peptide

<400>  27

Glu Phe Val Ala Thr Ile Asp Trp Gly Asp Gly Gly Ala Arg 
1               5                   10                  


<210>  28
<211>  11
<212>  PRT
<213>  Artificial

<220>
<223>  IGF1R-5 peptide

<400>  28

Thr Ile Asp Asn Tyr Ala Met Ala Trp Ser Arg 
1               5                   10      


<210>  29
<211>  16
<212>  PRT
<213>  Artificial

<220>
<223>  IGF1R-5 peptide

<400>  29

Leu Glu Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly Ser Leu Arg 
1               5                   10                  15      


<210>  30
<211>  15
<212>  PRT
<213>  Artificial

<220>
<223>  FC5  and FC5_ILIS peptide

<400>  30

Ile Thr Trp Gly Gly Asp Asn Thr Phe Tyr Ser Asn Ser Val Lys 
1               5                   10                  15  


<210>  31
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  A20.1 peptide

<400>  31

Thr Thr Tyr Tyr Ala Asp Ser Val Lys 
1               5                   


<210>  32
<211>  11
<212>  PRT
<213>  Artificial

<220>
<223>  A20.1 peptide

<400>  32

Glu Phe Val Ala Ala Gly Ser Ser Thr Gly Arg 
1               5                   10      


<210>  33
<211>  11
<212>  PRT
<213>  Artificial

<220>
<223>  A20.1 peptide

<400>  33

Thr Phe Ser Met Asp Pro Met Ala Trp Phe Arg 
1               5                   10      


<210>  34
<211>  26
<212>  PRT
<213>  Artificial

<220>
<223>  A20.1 peptide

<400>  34

Asp Glu Tyr Ala Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
1               5                   10                  15      


Gly Gln Ala Gly Gln Gly Ser Glu Gln Lys 
            20                  25      


<210>  35
<211>  11
<212>  PRT
<213>  Artificial

<220>
<223>  EG2 peptide

<400>  35

Asp Phe Ser Asp Tyr Val Met Gly Trp Phe Arg 
1               5                   10      


<210>  36
<211>  16
<212>  PRT
<213>  Artificial

<220>
<223>  EG2 peptide

<400>  36

Leu Glu Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Asp Ser Leu Arg 
1               5                   10                  15      


<210>  37
<211>  32
<212>  PRT
<213>  Artificial

<220>
<223>  EG2 peptide

<400>  37

Asn Met Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala 
1               5                   10                  15      


Val Tyr Tyr Cys Ala Val Asn Ser Ala Gly Thr Tyr Val Ser Pro Arg 
            20                  25                  30          


<210>  38
<211>  14
<212>  PRT
<213>  Artificial

<220>
<223>  Albumin peptide

<400>  38

Ala Pro Gln Val Ser Thr Pro Thr Leu Val Glu Ala Ala Arg 
1               5                   10                  


<210>  39
<211>  29
<212>  PRT
<213>  Artificial

<220>
<223>  Galanin-cysteamide

<400>  39

Gly Trp Thr Leu Asn Ser Ala Gly Tyr Leu Leu Gly Pro His Ala Ile 
1               5                   10                  15      


Asp Asn His Arg Ser Phe Ser Asp Lys His Gly Leu Thr 
            20                  25                  


<210>  40
<211>  19
<212>  PRT
<213>  Artificial

<220>
<223>  Signal peptide

<400>  40

Met Glu Phe Gly Leu Ser Trp Val Phe Leu Val Ala Ile Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys 
            


<210>  41
<211>  370
<212>  PRT
<213>  Artificial

<220>
<223>  IGF1R-5-mFc consensus fusion


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa is Glu or Gln

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa is Lys or Gln

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa is Val or Glu

<220>
<221>  MISC_FEATURE
<222>  (14)..(14)
<223>  Xaa is Ala or Pro

<220>
<221>  MISC_FEATURE
<222>  (37)..(37)
<223>  Xaa is Val or Ser

<220>
<221>  MISC_FEATURE
<222>  (44)..(44)
<223>  Xaa is Asp or Gly

<220>
<221>  MISC_FEATURE
<222>  (45)..(45)
<223>  Xaa is Leu or Arg

<220>
<221>  MISC_FEATURE
<222>  (47)..(47)
<223>  Xaa is Phe or Trp

<220>
<221>  MISC_FEATURE
<222>  (49)..(49)
<223>  Xaa is Ala or Ser

<220>
<221>  MISC_FEATURE
<222>  (58)..(58)
<223>  Xaa is Ala or Thr

<220>
<221>  MISC_FEATURE
<222>  (75)..(75)
<223>  Xaa is Ala or Ser

<220>
<221>  MISC_FEATURE
<222>  (77)..(77)
<223>  Xaa is Gly or Asn

<220>
<221>  MISC_FEATURE
<222>  (79)..(79)
<223>  Xaa is Met or Leu

<220>
<221>  MISC_FEATURE
<222>  (85)..(85)
<223>  Xaa is Asn or Arg

<220>
<221>  MISC_FEATURE
<222>  (87)..(87)
<223>  Xaa is Glu or Arg

<220>
<221>  MISC_FEATURE
<222>  (88)..(88)
<223>  Xaa is Pro or Ala

<220>
<221>  MISC_FEATURE
<222>  (95)..(95)
<223>  Xaa is Ser or Tyr

<220>
<221>  MISC_FEATURE
<222>  (119)..(119)
<223>  Xaa is Gln or Leu

<400>  41

Xaa Val Xaa Leu Xaa Glu Ser Gly Gly Gly Leu Val Gln Xaa Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Ile Asp Asn Tyr 
            20                  25                  30          


Ala Met Ala Trp Xaa Arg Gln Ala Pro Gly Lys Xaa Xaa Glu Xaa Val 
        35                  40                  45              


Xaa Thr Ile Asp Trp Gly Asp Gly Gly Xaa Arg Tyr Ala Asn Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Xaa Lys Xaa Thr Xaa Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Xaa Leu Xaa Xaa Glu Asp Thr Ala Val Tyr Xaa Cys 
                85                  90                  95      


Ala Met Ala Arg Gln Ser Arg Val Asn Leu Asp Val Ala Arg Tyr Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Xaa Val Thr Val Ser Ser Met Thr Val Asp 
        115                 120                 125             


Lys Lys Leu Glu Pro Ser Gly Pro Ile Ser Thr Ile Asn Pro Cys Pro 
    130                 135                 140                 


Pro Cys Lys Glu Cys His Lys Cys Pro Ala Pro Asn Leu Glu Gly Gly 
145                 150                 155                 160 


Pro Ser Val Phe Ile Phe Pro Pro Asn Ile Lys Asp Val Leu Met Ile 
                165                 170                 175     


Ser Leu Thr Pro Lys Val Thr Cys Val Val Val Asp Val Ser Glu Asp 
            180                 185                 190         


Asp Pro Asp Val Gln Ile Ser Trp Phe Val Asn Asn Val Glu Val His 
        195                 200                 205             


Thr Ala Gln Thr Gln Thr His Arg Glu Asp Tyr Asn Ser Thr Ile Arg 
    210                 215                 220                 


Val Val Ser Thr Leu Pro Ile Gln His Gln Asp Trp Met Ser Gly Lys 
225                 230                 235                 240 


Glu Phe Lys Cys Lys Val Asn Asn Lys Asp Leu Pro Ser Pro Ile Glu 
                245                 250                 255     


Arg Thr Ile Ser Lys Ile Lys Gly Leu Val Arg Ala Pro Gln Val Tyr 
            260                 265                 270         


Ile Leu Pro Pro Pro Ala Glu Gln Leu Ser Arg Lys Asp Val Ser Leu 
        275                 280                 285             


Thr Cys Leu Val Val Gly Phe Asn Pro Gly Asp Ile Ser Val Glu Trp 
    290                 295                 300                 


Thr Ser Asn Gly His Thr Glu Glu Asn Tyr Lys Asp Thr Ala Pro Val 
305                 310                 315                 320 


Leu Asp Ser Asp Gly Ser Tyr Phe Ile Tyr Ser Lys Leu Asn Met Lys 
                325                 330                 335     


Thr Ser Lys Trp Glu Lys Thr Asp Ser Phe Ser Cys Asn Val Arg His 
            340                 345                 350         


Glu Gly Leu Lys Asn Tyr Tyr Leu Lys Lys Thr Ile Ser Arg Ser Pro 
        355                 360                 365             


Gly Lys 
    370 


<210>  42
<211>  371
<212>  PRT
<213>  Artificial

<220>
<223>  mFc-IGF1R-5 consensus fusion


<220>
<221>  MISC_FEATURE
<222>  (248)..(248)
<223>  Xaa is Glu or Gln

<220>
<221>  MISC_FEATURE
<222>  (250)..(250)
<223>  Xaa is Lys or Gln

<220>
<221>  MISC_FEATURE
<222>  (252)..(252)
<223>  Xaa is Val or Glu

<220>
<221>  MISC_FEATURE
<222>  (261)..(261)
<223>  Xaa is Ala or Pro

<220>
<221>  MISC_FEATURE
<222>  (284)..(284)
<223>  Xaa is Val or Ser

<220>
<221>  MISC_FEATURE
<222>  (291)..(291)
<223>  Xaa is Asp or Gly

<220>
<221>  MISC_FEATURE
<222>  (292)..(292)
<223>  Xaa is Leu or Arg

<220>
<221>  MISC_FEATURE
<222>  (294)..(294)
<223>  Xaa is Phe or Trp

<220>
<221>  MISC_FEATURE
<222>  (296)..(296)
<223>  Xaa is Ala or Ser

<220>
<221>  MISC_FEATURE
<222>  (305)..(305)
<223>  Xaa is Ala or Thr

<220>
<221>  MISC_FEATURE
<222>  (322)..(322)
<223>  Xaa is Ala or Ser

<220>
<221>  MISC_FEATURE
<222>  (324)..(324)
<223>  Xaa is Ala or Ser

<220>
<221>  MISC_FEATURE
<222>  (326)..(326)
<223>  Xaa is Gly or Asn

<220>
<221>  MISC_FEATURE
<222>  (332)..(332)
<223>  Xaa is Met or Leu

<220>
<221>  MISC_FEATURE
<222>  (332)..(332)
<223>  Xaa is Asn or Arg

<220>
<221>  MISC_FEATURE
<222>  (334)..(334)
<223>  Xaa is Glu or Arg

<220>
<221>  MISC_FEATURE
<222>  (335)..(335)
<223>  Xaa is Pro or Ala

<220>
<221>  MISC_FEATURE
<222>  (342)..(342)
<223>  Xaa is Ser or Tyr

<220>
<221>  MISC_FEATURE
<222>  (366)..(366)
<223>  Xaa is Gln or Leu

<400>  42

Glu Pro Ser Gly Pro Ile Ser Thr Ile Asn Pro Cys Pro Pro Cys Lys 
1               5                   10                  15      


Glu Cys His Lys Cys Pro Ala Pro Asn Leu Glu Gly Gly Pro Ser Val 
            20                  25                  30          


Phe Ile Phe Pro Pro Asn Ile Lys Asp Val Leu Met Ile Ser Leu Thr 
        35                  40                  45              


Pro Lys Val Thr Cys Val Val Val Asp Val Ser Glu Asp Asp Pro Asp 
    50                  55                  60                  


Val Gln Ile Ser Trp Phe Val Asn Asn Val Glu Val His Thr Ala Gln 
65                  70                  75                  80  


Thr Gln Thr His Arg Glu Asp Tyr Asn Ser Thr Ile Arg Val Val Ser 
                85                  90                  95      


Thr Leu Pro Ile Gln His Gln Asp Trp Met Ser Gly Lys Glu Phe Lys 
            100                 105                 110         


Cys Lys Val Asn Asn Lys Asp Leu Pro Ser Pro Ile Glu Arg Thr Ile 
        115                 120                 125             


Ser Lys Ile Lys Gly Leu Val Arg Ala Pro Gln Val Tyr Ile Leu Pro 
    130                 135                 140                 


Pro Pro Ala Glu Gln Leu Ser Arg Lys Asp Val Ser Leu Thr Cys Leu 
145                 150                 155                 160 


Val Val Gly Phe Asn Pro Gly Asp Ile Ser Val Glu Trp Thr Ser Asn 
                165                 170                 175     


Gly His Thr Glu Glu Asn Tyr Lys Asp Thr Ala Pro Val Leu Asp Ser 
            180                 185                 190         


Asp Gly Ser Tyr Phe Ile Tyr Ser Lys Leu Asn Met Lys Thr Ser Lys 
        195                 200                 205             


Trp Glu Lys Thr Asp Ser Phe Ser Cys Asn Val Arg His Glu Gly Leu 
    210                 215                 220                 


Lys Asn Tyr Tyr Leu Lys Lys Thr Ile Ser Arg Ser Pro Gly Gly Gly 
225                 230                 235                 240 


Gly Ser Gly Gly Gly Gly Ser Xaa Val Xaa Leu Xaa Glu Ser Gly Gly 
                245                 250                 255     


Gly Leu Val Gln Xaa Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser 
            260                 265                 270         


Gly Arg Thr Ile Asp Asn Tyr Ala Met Ala Trp Xaa Arg Gln Ala Pro 
        275                 280                 285             


Gly Lys Xaa Xaa Glu Xaa Val Xaa Thr Ile Asp Trp Gly Asp Gly Gly 
    290                 295                 300                 


Xaa Arg Tyr Ala Asn Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp 
305                 310                 315                 320 


Asn Xaa Lys Xaa Thr Xaa Tyr Leu Gln Met Asn Xaa Leu Xaa Xaa Glu 
                325                 330                 335     


Asp Thr Ala Val Tyr Xaa Cys Ala Met Ala Arg Gln Ser Arg Val Asn 
            340                 345                 350         


Leu Asp Val Ala Arg Tyr Asp Tyr Trp Gly Gln Gly Thr Xaa Val Thr 
        355                 360                 365             


Val Ser Ser 
    370     


