                         SEQUENCE LISTING

<110>  Variation Biotechnologies Inc.
 
<120>  COMPOSITIONS AND METHODS FOR TREATMENT OF HEPATITIS C

<130>  PAT 103304W-90

<140>  PCT/IB2015/000397
<141>  2015-02-24

<150>  61/944,264
<151>  2014-02-25

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  538
<212>  PRT
<213>  Moloney Murine leukemia virus

<220>
<223>  Gag amino acid sequence

<400>  1

Met Gly Gln Thr Val Thr Thr Pro Leu Ser Leu Thr Leu Gly His Trp 
1               5                   10                  15      


Lys Asp Val Glu Arg Ile Ala His Asn Gln Ser Val Asp Val Lys Lys 
            20                  25                  30          


Arg Arg Trp Val Thr Phe Cys Ser Ala Glu Trp Pro Thr Phe Asn Val 
        35                  40                  45              


Gly Trp Pro Arg Asp Gly Thr Phe Asn Arg Asp Leu Ile Thr Gln Val 
    50                  55                  60                  


Lys Ile Lys Val Phe Ser Pro Gly Pro His Gly His Pro Asp Gln Val 
65                  70                  75                  80  


Pro Tyr Ile Val Thr Trp Glu Ala Leu Ala Phe Asp Pro Pro Pro Trp 
                85                  90                  95      


Val Lys Pro Phe Val His Pro Lys Pro Pro Pro Pro Leu Pro Pro Ser 
            100                 105                 110         


Ala Pro Ser Leu Pro Leu Glu Pro Pro Arg Ser Thr Pro Pro Arg Ser 
        115                 120                 125             


Ser Leu Tyr Pro Ala Leu Thr Pro Ser Leu Gly Ala Lys Pro Lys Pro 
    130                 135                 140                 


Gln Val Leu Ser Asp Ser Gly Gly Pro Leu Ile Asp Leu Leu Thr Glu 
145                 150                 155                 160 


Asp Pro Pro Pro Tyr Arg Asp Pro Arg Pro Pro Pro Ser Asp Arg Asp 
                165                 170                 175     


Gly Asn Gly Gly Glu Ala Thr Pro Ala Gly Glu Ala Pro Asp Pro Ser 
            180                 185                 190         


Pro Met Ala Ser Arg Leu Arg Gly Arg Arg Glu Pro Pro Val Ala Asp 
        195                 200                 205             


Ser Thr Thr Ser Gln Ala Phe Pro Leu Arg Ala Gly Gly Asn Gly Gln 
    210                 215                 220                 


Leu Gln Tyr Trp Pro Phe Ser Ser Ser Asp Leu Tyr Asn Trp Lys Asn 
225                 230                 235                 240 


Asn Asn Pro Ser Phe Ser Glu Asp Pro Gly Lys Leu Thr Ala Leu Ile 
                245                 250                 255     


Glu Ser Val Leu Ile Thr His Gln Pro Thr Trp Asp Asp Cys Gln Gln 
            260                 265                 270         


Leu Leu Gly Thr Leu Leu Thr Gly Glu Glu Lys Gln Arg Val Leu Leu 
        275                 280                 285             


Glu Ala Arg Lys Ala Val Arg Gly Asp Asp Gly Arg Pro Thr Gln Leu 
    290                 295                 300                 


Pro Asn Glu Val Asp Ala Ala Phe Pro Leu Glu Arg Pro Asp Trp Asp 
305                 310                 315                 320 


Tyr Thr Thr Gln Ala Gly Arg Asn His Leu Val His Tyr Arg Gln Leu 
                325                 330                 335     


Leu Leu Ala Gly Leu Gln Asn Ala Gly Arg Ser Pro Thr Asn Leu Ala 
            340                 345                 350         


Lys Val Lys Gly Ile Thr Gln Gly Pro Asn Glu Ser Pro Ser Ala Phe 
        355                 360                 365             


Leu Glu Arg Leu Lys Glu Ala Tyr Arg Arg Tyr Thr Pro Tyr Asp Pro 
    370                 375                 380                 


Glu Asp Pro Gly Gln Glu Thr Asn Val Ser Met Ser Phe Ile Trp Gln 
385                 390                 395                 400 


Ser Ala Pro Asp Ile Gly Arg Lys Leu Glu Arg Leu Glu Asp Leu Lys 
                405                 410                 415     


Asn Lys Thr Leu Gly Asp Leu Val Arg Glu Ala Glu Lys Ile Phe Asn 
            420                 425                 430         


Lys Arg Glu Thr Pro Glu Glu Arg Glu Glu Arg Ile Arg Arg Glu Thr 
        435                 440                 445             


Glu Glu Lys Glu Glu Arg Arg Arg Thr Glu Asp Glu Gln Lys Glu Lys 
    450                 455                 460                 


Glu Arg Asp Arg Arg Arg His Arg Glu Met Ser Lys Leu Leu Ala Thr 
465                 470                 475                 480 


Val Val Ser Gly Gln Lys Gln Asp Arg Gln Gly Gly Glu Arg Arg Arg 
                485                 490                 495     


Ser Gln Leu Asp Arg Asp Gln Cys Ala Tyr Cys Lys Glu Lys Gly His 
            500                 505                 510         


Trp Ala Lys Asp Cys Pro Lys Lys Pro Arg Gly Pro Arg Gly Pro Arg 
        515                 520                 525             


Pro Gln Thr Ser Leu Leu Thr Leu Asp Asp 
    530                 535             


<210>  2
<211>  1614
<212>  DNA
<213>  Moloney Murine leukemia virus

<220>
<223>  Gag nucleotide sequence

<400>  2
atgggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag       60

cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct      120

gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc      180

atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc      240

ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt      300

gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct      360

cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc      420

aaacctaaac ctcaagttct ttctgacagt ggggggccgc tcatcgacct acttacagaa      480

gaccccccgc cttataggga cccaagacca cccccttccg acagggacgg aaatggtgga      540

gaagcgaccc ctgcgggaga ggcaccggac ccctccccaa tggcatctcg cctacgtggg      600

agacgggagc cccctgtggc cgactccact acctcgcagg cattccccct ccgcgcagga      660

ggaaacggac agcttcaata ctggccgttc tcctcttctg acctttacaa ctggaaaaat      720

aataaccctt ctttttctga agatccaggt aaactgacag ctctgatcga gtctgttctc      780

atcacccatc agcccacctg ggacgactgt cagcagctgt tggggactct gctgaccgga      840

gaagaaaaac aacgggtgct cttagaggct agaaaggcgg tgcggggcga tgatgggcgc      900

cccactcaac tgcccaatga agtcgatgcc gcttttcccc tcgagcgccc agactgggat      960

tacaccaccc aggcaggtag gaaccaccta gtccactatc gccagttgct cctagcgggt     1020

ctccaaaacg cgggcagaag ccccaccaat ttggccaagg taaaaggaat aacacaaggg     1080

cccaatgagt ctccctcggc cttcctagag agacttaagg aagcctatcg caggtacact     1140

ccttatgacc ctgaggaccc agggcaagaa actaatgtgt ctatgtcttt catttggcag     1200

tctgccccag acattgggag aaagttagag aggttagaag atttaaaaaa caagacgctt     1260

ggagatttgg ttagagaggc agaaaagatc tttaataaac gagaaacccc ggaagaaaga     1320

gaggaacgta tcaggagaga aacagaggaa aaagaagaac gccgtaggac agaggatgag     1380

cagaaagaga aagaaagaga tcgtaggaga catagagaga tgagcaagct attggccact     1440

gtcgttagtg gacagaaaca ggatagacag ggaggagaac gaaggaggtc ccaactcgat     1500

cgcgaccagt gtgcctactg caaagaaaag gggcactggg ctaaagattg tcccaagaaa     1560

ccacgaggac ctcggggacc aagaccccag acctccctcc tgaccctaga tgac           1614


<210>  3
<211>  1614
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized Moloney Murine leukemia virus Gag nucleotide 
       sequence

<400>  3
atgggacaga ccgtcacaac acccctgagc ctgaccctgg gacattggaa agacgtggag       60

aggatcgcac ataaccagag cgtggacgtg aagaaacgga gatgggtcac attctgcagt      120

gctgagtggc caacttttaa tgtgggatgg ccccgagacg gcactttcaa cagggatctg      180

atcacccagg tgaagatcaa ggtctttagc ccaggacctc acggacatcc agaccaggtg      240

ccttatatcg tcacctggga ggcactggcc ttcgatcccc ctccatgggt gaagccattt      300

gtccacccaa aaccacctcc accactgcct ccaagtgccc cttcactgcc actggaacca      360

ccccggagca caccaccccg cagctccctg tatcctgctc tgactccatc tctgggcgca      420

aagccaaaac cacaggtgct gagcgactcc ggaggaccac tgattgacct gctgacagag      480

gaccccccac cataccgaga tcctcggcct ccaccaagcg accgcgatgg aaatggagga      540

gaggctactc ctgccggcga agcccctgac ccatctccaa tggctagtag gctgcgcggc      600

aggcgcgagc ctccagtggc agatagcacc acatcccagg ccttccctct gagggctggg      660

ggaaatgggc agctccagta ttggccattt tctagttcag acctgtacaa ctggaagaac      720

aataacccct ctttcagtga ggaccccggc aaactgaccg ccctgatcga atccgtgctg      780

attacccatc agcccacatg ggacgattgt cagcagctcc tgggcaccct gctgaccgga      840

gaggaaaagc agcgcgtgct gctggaggct cgcaaagcag tccgagggga cgatggacgg      900

cccacacagc tccctaatga ggtggacgcc gcttttccac tggaaagacc cgactgggat      960

tatactaccc aggcagggag aaaccacctg gtccattaca ggcagctcct gctggcaggc     1020

ctgcagaatg ccgggagatc ccccaccaac ctggccaagg tgaaaggcat cacacagggg     1080

cctaatgagt caccaagcgc ctttctggag aggctgaagg aagcttaccg acggtatacc     1140

ccatacgacc ctgaggaccc cggacaggaa acaaacgtct ccatgtcttt catctggcag     1200

tctgccccag acattgggcg gaagctggag agactggaag acctgaagaa caagaccctg     1260

ggcgacctgg tgcgggaggc tgaaaagatc ttcaacaaac gggagacccc cgaggaaaga     1320

gaggaaagga ttagaaggga aactgaggaa aaggaggaac gccgacggac cgaggacgaa     1380

cagaaggaga aagaacgaga tcggcggcgg caccgggaga tgtcaaagct gctggccacc     1440

gtggtcagcg gacagaaaca ggacagacag ggaggagagc gacggagaag ccagctcgac     1500

agggatcagt gcgcatactg taaggaaaaa ggccattggg ccaaggattg ccccaaaaag     1560

ccaagaggac caagaggacc aagaccacag acatcactgc tgaccctgga cgac           1614


<210>  4
<211>  387
<212>  PRT
<213>  Hepatitis C virus

<220>
<223>  E2-G amino acid sequence

<400>  4

Met Gly Tyr Ile Pro Val Val Gly Ala Pro Leu Ser Gly Ala Ala Arg 
1               5                   10                  15      


Ala Val Ala His Gly Val Arg Val Leu Glu Asp Gly Val Asn Tyr Ala 
            20                  25                  30          


Thr Gly Asn Leu Pro Gly Phe Pro Phe Ser Ile Phe Leu Leu Ala Leu 
        35                  40                  45              


Leu Ser Cys Ile Thr Val Pro Val Ser Ala Gly Thr Thr Thr Val Gly 
    50                  55                  60                  


Gly Ala Val Ala Arg Ser Thr Asn Val Ile Ala Gly Val Phe Ser His 
65                  70                  75                  80  


Gly Pro Gln Gln Asn Ile Gln Leu Ile Asn Thr Asn Gly Ser Trp His 
                85                  90                  95      


Ile Asn Arg Thr Ala Leu Asn Cys Asn Asp Ser Leu Asn Thr Gly Phe 
            100                 105                 110         


Leu Ala Ala Leu Phe Tyr Thr Asn Arg Phe Asn Ser Ser Gly Cys Pro 
        115                 120                 125             


Gly Arg Leu Ser Ala Cys Arg Asn Ile Glu Ala Phe Arg Ile Gly Trp 
    130                 135                 140                 


Gly Thr Leu Gln Tyr Glu Asp Asn Val Thr Asn Pro Glu Asp Met Arg 
145                 150                 155                 160 


Pro Tyr Cys Trp His Tyr Pro Pro Lys Pro Cys Gly Val Val Pro Ala 
                165                 170                 175     


Arg Ser Val Cys Gly Pro Val Tyr Cys Phe Thr Pro Ser Pro Val Val 
            180                 185                 190         


Val Gly Thr Thr Asp Arg Arg Gly Val Pro Thr Tyr Thr Trp Gly Glu 
        195                 200                 205             


Asn Glu Thr Asp Val Phe Leu Leu Asn Ser Thr Arg Pro Pro Gln Gly 
    210                 215                 220                 


Ser Trp Phe Gly Cys Thr Trp Met Asn Ser Thr Gly Phe Thr Lys Thr 
225                 230                 235                 240 


Cys Gly Ala Pro Pro Cys Arg Thr Arg Ala Asp Phe Asn Ala Ser Thr 
                245                 250                 255     


Asp Leu Leu Cys Pro Thr Asp Cys Phe Arg Lys His Pro Asp Ala Thr 
            260                 265                 270         


Tyr Ile Lys Cys Gly Ser Gly Pro Trp Leu Thr Pro Lys Cys Leu Val 
        275                 280                 285             


His Tyr Pro Tyr Arg Leu Trp His Tyr Pro Cys Thr Val Asn Phe Thr 
    290                 295                 300                 


Ile Phe Lys Ile Arg Met Tyr Val Gly Gly Val Glu His Arg Leu Thr 
305                 310                 315                 320 


Ala Ala Cys Asn Phe Thr Arg Gly Asp Arg Cys Asp Leu Glu Asp Arg 
                325                 330                 335     


Asp Arg Ser Gln Leu Ser Pro Phe Phe Phe Ile Ile Gly Leu Ile Ile 
            340                 345                 350         


Gly Leu Phe Leu Val Leu Arg Val Gly Ile His Leu Cys Ile Lys Leu 
        355                 360                 365             


Lys His Thr Lys Lys Arg Gln Ile Tyr Thr Asp Ile Glu Met Asn Arg 
    370                 375                 380                 


Leu Gly Lys 
385         


<210>  5
<211>  1164
<212>  DNA
<213>  Hepatitis C virus

<220>
<223>  E2-G nucleotide sequence

<400>  5
atggggtaca tccccgtcgt aggcgccccg cttagtggcg ccgccagagc tgtcgcgcac       60

ggcgtgagag tcctggagga cggggttaat tatgcaacag ggaacctacc cggtttcccc      120

ttttctatct tcttgctggc cctgttgtcc tgcatcaccg ttccggtctc tgctggcacc      180

accaccgttg gaggcgctgt tgcacgttcc accaacgtga ttgccggcgt gttcagccat      240

ggccctcagc agaacattca gctcattaac accaacggca gttggcacat caaccgtact      300

gccttgaatt gcaatgactc cttgaacacc ggctttctcg cggccttgtt ctacaccaac      360

cgctttaact cgtcagggtg tccagggcgc ctgtccgcct gccgcaacat cgaggctttc      420

cggatagggt ggggcaccct acagtacgag gataatgtca ccaatccaga ggatatgagg      480

ccgtactgct ggcactaccc cccaaagccg tgtggcgtag tccccgcgag gtctgtgtgt      540

ggcccagtgt actgtttcac ccccagcccg gtagtagtgg gcacgaccga cagacgtgga      600

gtgcccacct acacatgggg agagaatgag acagatgtct tcctactgaa cagcacccga      660

ccgccgcagg gctcatggtt cggctgcacg tggatgaact ccactggttt caccaagact      720

tgtggcgcgc caccttgccg caccagagct gacttcaacg ccagcacgga cttgttgtgc      780

cctacggatt gttttaggaa gcatcctgat gccacttata ttaagtgtgg ttctgggccc      840

tggctcacac caaagtgcct ggtccactac ccttacagac tctggcatta cccctgcaca      900

gtcaatttta ccatcttcaa gataagaatg tatgtagggg gggttgagca caggctcacg      960

gccgcatgca acttcactcg tggggatcgc tgcgacttgg aggacaggga caggagtcag     1020

ctgtctcctt ttttctttat catagggtta atcattggac tattcttggt tctccgagtt     1080

ggtatccatc tttgcattaa attaaagcac accaagaaaa gacagattta tacagacata     1140

gagatgaacc gacttggaaa gtaa                                            1164


<210>  6
<211>  1164
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized E2-G nucleotide sequence

<400>  6
atgggctaca tccctgtcgt cggggcacca ctgtctgggg ctgcacgggc tgtcgcacac       60

ggggtccggg tcctggaaga tggagtcaac tacgccaccg ggaatctgcc aggatttccc      120

ttctccatct tcctgctggc tctgctgtca tgcattacag tccctgtgag cgcaggcacc      180

acaactgtcg gaggggctgt ggcaaggagt accaacgtca tcgccggggt gttttcacac      240

ggaccacagc agaatatcca gctcatcaac accaatggct cttggcatat taaccgcaca      300

gctctgaact gtaatgatag tctgaatact gggtttctgg ccgctctgtt ctacaccaac      360

cggttcaaca gcagcggatg ccccggcaga ctgtctgcat gtcgaaacat cgaggccttc      420

cgcattgggt ggggaactct gcagtatgag gataacgtga ccaatcccga agacatgagg      480

ccttactgct ggcactatcc acctaagcca tgtggagtgg tccccgcacg cagcgtctgc      540

ggaccagtgt actgtttcac accctcccct gtggtcgtgg gcaccacaga tcggagaggg      600

gtgcccactt atacctgggg cgagaacgaa acagacgtgt tcctgctgaa cagcactagg      660

ccaccccagg gctcttggtt cgggtgcact tggatgaact caaccggctt caccaaaact      720

tgcggcgcac ctccatgtag gacccgcgcc gacttcaatg ctagtacaga tctgctgtgc      780

cccactgact gttttcggaa gcatcctgac gctacatata tcaaatgcgg cagcgggcct      840

tggctgactc caaagtgtct ggtgcactac ccttatagac tgtggcatta cccatgcacc      900

gtgaacttta caatcttcaa aatccggatg tacgtcggag gcgtggagca cagactgacc      960

gcagcctgca atttcacaag aggcgacagg tgtgatctgg aagaccgaga tcggtctcag     1020

ctcagtcctt tctttttcat cattggactg atcattggcc tgtttctggt cctgagagtg     1080

ggcatccacc tgtgcattaa gctgaaacat accaaaaaac gacagatcta caccgacatc     1140

gaaatgaaca gactgggcaa ataa                                            1164


<210>  7
<211>  1033
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Gag/NS3-NS4 amino acid sequence

<400>  7

Met Gly Gln Thr Val Thr Thr Pro Leu Ser Leu Thr Leu Gly His Trp 
1               5                   10                  15      


Lys Asp Val Glu Arg Ile Ala His Asn Gln Ser Val Asp Val Lys Lys 
            20                  25                  30          


Arg Arg Trp Val Thr Phe Cys Ser Ala Glu Trp Pro Thr Phe Asn Val 
        35                  40                  45              


Gly Trp Pro Arg Asp Gly Thr Phe Asn Arg Asp Leu Ile Thr Gln Val 
    50                  55                  60                  


Lys Ile Lys Val Phe Ser Pro Gly Pro His Gly His Pro Asp Gln Val 
65                  70                  75                  80  


Pro Tyr Ile Val Thr Trp Glu Ala Leu Ala Phe Asp Pro Pro Pro Trp 
                85                  90                  95      


Val Lys Pro Phe Val His Pro Lys Pro Pro Pro Pro Leu Pro Pro Ser 
            100                 105                 110         


Ala Pro Ser Leu Pro Leu Glu Pro Pro Arg Ser Thr Pro Pro Arg Ser 
        115                 120                 125             


Ser Leu Tyr Pro Ala Leu Thr Pro Ser Leu Gly Ala Lys Pro Lys Pro 
    130                 135                 140                 


Gln Val Leu Ser Asp Ser Gly Gly Pro Leu Ile Asp Leu Leu Thr Glu 
145                 150                 155                 160 


Asp Pro Pro Pro Tyr Arg Asp Pro Arg Pro Pro Pro Ser Asp Arg Asp 
                165                 170                 175     


Gly Asn Gly Gly Glu Ala Thr Pro Ala Gly Glu Ala Pro Asp Pro Ser 
            180                 185                 190         


Pro Met Ala Ser Arg Leu Arg Gly Arg Arg Glu Pro Pro Val Ala Asp 
        195                 200                 205             


Ser Thr Thr Ser Gln Ala Phe Pro Leu Arg Ala Gly Gly Asn Gly Gln 
    210                 215                 220                 


Leu Gln Tyr Trp Pro Phe Ser Ser Ser Asp Leu Tyr Asn Trp Lys Asn 
225                 230                 235                 240 


Asn Asn Pro Ser Phe Ser Glu Asp Pro Gly Lys Leu Thr Ala Leu Ile 
                245                 250                 255     


Glu Ser Val Leu Ile Thr His Gln Pro Thr Trp Asp Asp Cys Gln Gln 
            260                 265                 270         


Leu Leu Gly Thr Leu Leu Thr Gly Glu Glu Lys Gln Arg Val Leu Leu 
        275                 280                 285             


Glu Ala Arg Lys Ala Val Arg Gly Asp Asp Gly Arg Pro Thr Gln Leu 
    290                 295                 300                 


Pro Asn Glu Val Asp Ala Ala Phe Pro Leu Glu Arg Pro Asp Trp Asp 
305                 310                 315                 320 


Tyr Thr Thr Gln Ala Gly Arg Asn His Leu Val His Tyr Arg Gln Leu 
                325                 330                 335     


Leu Leu Ala Gly Leu Gln Asn Ala Gly Arg Ser Pro Thr Asn Leu Ala 
            340                 345                 350         


Lys Val Lys Gly Ile Thr Gln Gly Pro Asn Glu Ser Pro Ser Ala Phe 
        355                 360                 365             


Leu Glu Arg Leu Lys Glu Ala Tyr Arg Arg Tyr Thr Pro Tyr Asp Pro 
    370                 375                 380                 


Glu Asp Pro Gly Gln Glu Thr Asn Val Ser Met Ser Phe Ile Trp Gln 
385                 390                 395                 400 


Ser Ala Pro Asp Ile Gly Arg Lys Leu Glu Arg Leu Glu Asp Leu Lys 
                405                 410                 415     


Asn Lys Thr Leu Gly Asp Leu Val Arg Glu Ala Glu Lys Ile Phe Asn 
            420                 425                 430         


Lys Arg Glu Thr Pro Glu Glu Arg Glu Glu Arg Ile Arg Arg Glu Thr 
        435                 440                 445             


Glu Glu Lys Glu Glu Arg Arg Arg Thr Glu Asp Glu Gln Lys Glu Lys 
    450                 455                 460                 


Glu Arg Asp Arg Arg Arg His Arg Glu Met Ser Lys Leu Leu Ala Thr 
465                 470                 475                 480 


Val Val Ser Gly Gln Lys Gln Asp Arg Gln Gly Gly Glu Arg Arg Arg 
                485                 490                 495     


Ser Gln Leu Asp Arg Asp Gln Cys Ala Tyr Cys Lys Glu Lys Gly His 
            500                 505                 510         


Trp Ala Lys Asp Cys Pro Lys Lys Pro Arg Gly Pro Arg Gly Pro Arg 
        515                 520                 525             


Pro Gln Thr Ser Leu Leu Thr Leu Asp Asp Pro Ala Val Pro Gln Thr 
    530                 535                 540                 


Tyr Gln Val Gly Tyr Leu His Ala Pro Thr Gly Ser Gly Lys Ser Thr 
545                 550                 555                 560 


Lys Val Pro Val Ala Tyr Ala Ala Gln Gly Tyr Lys Val Leu Val Leu 
                565                 570                 575     


Asn Pro Ser Val Ala Ala Thr Leu Gly Phe Gly Ala Tyr Leu Ser Lys 
            580                 585                 590         


Ala His Gly Ile Asn Pro Asn Ile Arg Thr Gly Val Arg Thr Val Met 
        595                 600                 605             


Thr Gly Glu Ala Ile Thr Tyr Ser Thr Tyr Gly Lys Phe Leu Ala Asp 
    610                 615                 620                 


Gly Gly Cys Ala Ser Gly Ala Tyr Asp Ile Ile Ile Cys Asp Glu Cys 
625                 630                 635                 640 


His Ala Val Asp Ala Thr Ser Ile Leu Gly Ile Gly Thr Val Leu Asp 
                645                 650                 655     


Gln Ala Glu Thr Ala Gly Val Arg Leu Thr Val Leu Ala Thr Ala Thr 
            660                 665                 670         


Pro Pro Gly Ser Val Thr Thr Pro His Pro Asp Ile Glu Glu Val Gly 
        675                 680                 685             


Leu Gly Arg Glu Gly Glu Ile Pro Phe Tyr Gly Arg Ala Ile Pro Leu 
    690                 695                 700                 


Ser Cys Ile Lys Gly Gly Arg His Leu Ile Phe Cys His Ser Lys Lys 
705                 710                 715                 720 


Lys Cys Asp Glu Leu Ala Ala Ala Leu Arg Gly Met Gly Leu Asn Ala 
                725                 730                 735     


Val Ala Tyr Tyr Arg Gly Leu Asp Val Ser Ile Ile Pro Ala Gln Gly 
            740                 745                 750         


Asp Val Val Val Val Ala Thr Asp Ala Leu Met Thr Gly Tyr Thr Gly 
        755                 760                 765             


Asp Phe Asp Ser Val Ile Asp Cys Asn Val Ala Val Thr Gln Ala Val 
    770                 775                 780                 


Asp Phe Ser Leu Asp Pro Thr Phe Thr Ile Thr Thr Gln Thr Val Pro 
785                 790                 795                 800 


Gln Asp Ala Val Ser Arg Ser Gln Arg Arg Gly Arg Thr Gly Arg Gly 
                805                 810                 815     


Arg Gln Gly Thr Tyr Arg Tyr Val Ser Thr Gly Glu Arg Ala Ser Gly 
            820                 825                 830         


Met Phe Asp Ser Val Val Leu Cys Glu Cys Tyr Asp Ala Gly Ala Ala 
        835                 840                 845             


Trp Tyr Asp Leu Thr Pro Ala Glu Thr Thr Val Arg Leu Arg Ala Tyr 
    850                 855                 860                 


Phe Asn Thr Pro Gly Leu Pro Val Cys Gln Asp His Leu Glu Phe Trp 
865                 870                 875                 880 


Glu Ala Val Phe Thr Gly Leu Thr His Ile Asp Ala His Phe Leu Ser 
                885                 890                 895     


Gln Thr Lys Gln Ala Gly Glu Asn Phe Ala Tyr Leu Val Ala Tyr Gln 
            900                 905                 910         


Ala Thr Val Cys Ala Arg Ala Lys Ala Pro Pro Pro Ser Trp Asp Ala 
        915                 920                 925             


Met Trp Lys Cys Leu Ala Arg Leu Lys Pro Thr Leu Ala Gly Pro Thr 
    930                 935                 940                 


Pro Leu Leu Tyr Arg Leu Gly Pro Ile Thr Asn Glu Val Thr Leu Thr 
945                 950                 955                 960 


His Pro Gly Thr Lys Tyr Ile Ala Thr Cys Met Gln Ala Asp Leu Glu 
                965                 970                 975     


Val Met Thr Ser Thr Trp Val Leu Ala Gly Gly Val Leu Ala Ala Val 
            980                 985                 990         


Ala Ala Tyr Cys Leu Ala Thr Gly  Cys Val Ser Ile Ile  Gly Arg Leu 
        995                 1000                 1005             


His Val  Asn Gln Arg Val Val  Val Ala Pro Asp Lys  Glu Val Leu 
    1010                 1015                 1020             


Tyr Glu  Ala Phe Asp Glu Met  Glu Glu Cys 
    1025                 1030             


<210>  8
<211>  3102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gag/NS3-NS4 nucleotide sequence

<400>  8
atgggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag       60

cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct      120

gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc      180

atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc      240

ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt      300

gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct      360

cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc      420

aaacctaaac ctcaagttct ttctgacagt ggggggccgc tcatcgacct acttacagaa      480

gaccccccgc cttataggga cccaagacca cccccttccg acagggacgg aaatggtgga      540

gaagcgaccc ctgcgggaga ggcaccggac ccctccccaa tggcatctcg cctacgtggg      600

agacgggagc cccctgtggc cgactccact acctcgcagg cattccccct ccgcgcagga      660

ggaaacggac agcttcaata ctggccgttc tcctcttctg acctttacaa ctggaaaaat      720

aataaccctt ctttttctga agatccaggt aaactgacag ctctgatcga gtctgttctc      780

atcacccatc agcccacctg ggacgactgt cagcagctgt tggggactct gctgaccgga      840

gaagaaaaac aacgggtgct cttagaggct agaaaggcgg tgcggggcga tgatgggcgc      900

cccactcaac tgcccaatga agtcgatgcc gcttttcccc tcgagcgccc agactgggat      960

tacaccaccc aggcaggtag gaaccaccta gtccactatc gccagttgct cctagcgggt     1020

ctccaaaacg cgggcagaag ccccaccaat ttggccaagg taaaaggaat aacacaaggg     1080

cccaatgagt ctccctcggc cttcctagag agacttaagg aagcctatcg caggtacact     1140

ccttatgacc ctgaggaccc agggcaagaa actaatgtgt ctatgtcttt catttggcag     1200

tctgccccag acattgggag aaagttagag aggttagaag atttaaaaaa caagacgctt     1260

ggagatttgg ttagagaggc agaaaagatc tttaataaac gagaaacccc ggaagaaaga     1320

gaggaacgta tcaggagaga aacagaggaa aaagaagaac gccgtaggac agaggatgag     1380

cagaaagaga aagaaagaga tcgtaggaga catagagaga tgagcaagct attggccact     1440

gtcgttagtg gacagaaaca ggatagacag ggaggagaac gaaggaggtc ccaactcgat     1500

cgcgaccagt gtgcctactg caaagaaaag gggcactggg ctaaagattg tcccaagaaa     1560

ccacgaggac ctcggggacc aagaccccag acctccctcc tgaccctaga tgacccggct     1620

gtgccccaga cctatcaggt cgggtacttg catgctccaa ctggcagtgg aaagagcacc     1680

aaggtccctg tcgcgtatgc cgcccagggg tacaaagtac tagtgcttaa cccctcggta     1740

gctgccaccc tggggtttgg ggcgtaccta tccaaggcac atggcatcaa tcccaacatt     1800

aggactggag tcaggaccgt gatgaccggg gaggccatca cgtactccac atatggcaaa     1860

tttctcgccg atgggggctg cgctagcggc gcctatgaca tcatcatatg cgatgaatgc     1920

cacgctgtgg atgctacctc cattctcggc atcggaacgg tccttgatca agcagagaca     1980

gccggggtca gactaactgt gctggctacg gccacacccc ccgggtcagt gacaaccccc     2040

catcccgata tagaagaggt aggcctcggg cgggagggtg agatcccctt ctatgggagg     2100

gcgattcccc tatcctgcat caagggaggg agacacctga ttttctgcca ctcaaagaaa     2160

aagtgtgacg agctcgcggc ggcccttcgg ggcatgggct tgaatgccgt ggcatactat     2220

agagggttgg acgtctccat aataccagct cagggagatg tggtggtcgt cgccaccgac     2280

gccctcatga cggggtacac tggagacttt gactccgtga tcgactgcaa tgtagcggtc     2340

acccaagctg tcgacttcag cctggacccc accttcacta taaccacaca gactgtccca     2400

caagacgctg tctcacgcag tcagcgccgc gggcgcacag gtagaggaag acagggcact     2460

tataggtatg tttccactgg tgaacgagcc tcaggaatgt ttgacagtgt agtgctttgt     2520

gagtgctacg acgcaggggc tgcgtggtac gatctcacac cagcggagac caccgtcagg     2580

cttagagcgt atttcaacac gcccggccta cccgtgtgtc aagaccatct tgaattttgg     2640

gaggcagttt tcaccggcct cacacacata gacgcccact tcctctccca aacaaagcaa     2700

gcgggggaga acttcgcgta cctagtagcc taccaagcta cggtgtgcgc cagagccaag     2760

gcccctcccc cgtcctggga cgccatgtgg aagtgcctgg cccgactcaa gcctacgctt     2820

gcgggcccca cacctctcct gtaccgtttg ggccctatta ccaatgaggt caccctcaca     2880

caccctggga cgaagtacat cgccacatgc atgcaagctg accttgaggt catgaccagc     2940

acgtgggtcc tagctggagg agtcctggca gccgtcgccg catattgcct ggcgactgga     3000

tgcgtttcca tcatcggccg cttgcacgtc aaccagcgag tcgtcgttgc gccggataag     3060

gaggtcctgt atgaggcttt tgatgagatg gaggaatgct aa                        3102


<210>  9
<211>  3102
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Codon optimized Gag/NS3-NS4 nucleotide sequence

<400>  9
atggggcaga ccgtgactac accactgtcc ctgaccctgg ggcattggaa ggatgtcgaa       60

aggattgctc ataaccagag cgtggatgtg aagaaacgga gatgggtcac attctgcagt      120

gccgagtggc caacttttaa tgtgggatgg ccccgggacg ggaccttcaa cagagatctg      180

attacacagg tgaaaatcaa ggtctttagc ccaggaccac acggacatcc agaccaggtg      240

ccttacattg tcacctggga ggccctggct ttcgatcccc ctccatgggt gaaaccattt      300

gtccacccaa agccacctcc accactgcct ccaagcgccc cctccctgcc tctggaacca      360

cctcgcagta caccaccccg aagctccctg tatcctgcac tgactccaag cctgggggcc      420

aaacctaagc cacaggtgct gagcgactcc ggaggaccac tgatcgacct gctgaccgag      480

gaccccccac cataccggga cccccggccc ccacccagcg acagggatgg aaatggagga      540

gaggcaacac ctgccggcga agcacccgac ccttccccaa tggcatctcg actgcgggga      600

aggcgcgaac ctccagtggc agattctacc acaagtcagg cattcccact gcgagcagga      660

ggaaatggac agctccagta ttggcctttt tctagttcag acctgtacaa ctggaaaaac      720

aataacccct ctttcagtga ggaccccggc aagctgaccg ccctgattga atccgtgctg      780

atcacacacc agcccacttg ggacgattgc cagcagctcc tgggcaccct gctgaccggc      840

gaggaaaaac agagggtgct gctggaggca aggaaggctg tccgcgggga cgatggacgc      900

cccacccagc tccctaatga ggtggacgcc gcttttccac tggaaagacc cgactgggat      960

tatactaccc aggccggcag aaaccacctg gtgcattaca gacagctcct gctggctggg     1020

ctgcagaatg caggacggag ccccaccaac ctggccaaag tgaaggggat cacacaggga     1080

cctaatgagt caccaagcgc cttcctggag cggctgaagg aagcataccg acggtatacc     1140

ccatacgacc cagaggaccc cggacaggaa acaaacgtgt ccatgtcttt catttggcag     1200

agcgcccccg acatcggacg aaagctggag cggctggaag acctgaaaaa taagactctg     1260

ggcgatctgg tgcgggaggc cgaaaaaatt ttcaacaaga gagagacccc tgaggaaagg     1320

gaggaacgca tcagaaggga aactgaggaa aaggaggaac gccgacggac cgaggacgaa     1380

cagaaagaga aggaaaggga tcggcggcgg caccgggaga tgagcaaact gctggccacc     1440

gtggtctccg gacagaagca ggacaggcag ggaggagagc gacggagatc ccagctcgac     1500

cgcgatcagt gcgcatactg taaagaaaag ggccactggg ccaaggattg cccaaagaaa     1560

ccccgaggcc ctcgggggcc cagacctcag acctctctgc tgacactgga cgatcccgcc     1620

gtgccccaga cctatcaggt cggctacctg catgccccca ccggatcagg caaaagcaca     1680

aaggtgcctg tcgcctatgc agcccagggg tacaaagtgc tggtcctgaa cccttctgtg     1740

gctgcaacac tggggtttgg agcctatctg agtaaggctc atggcattaa tccaaacatc     1800

agaactggcg tgaggactgt catgaccggg gaggccatca catattcaac ttacgggaag     1860

ttcctggctg acggaggctg tgcaagcgga gcctacgaca tcattatctg cgatgagtgt     1920

cacgctgtgg acgcaacttc aattctgggc atcgggaccg tgctggatca ggccgaaact     1980

gctggcgtgc ggctgaccgt cctggcaaca gcaactcccc ctgggagcgt gacaactcca     2040

catcccgata ttgaggaagt cggactgggc agggagggag aaatcccttt ttatggccgc     2100

gccattccac tgtcctgcat caagggggga agacacctga tcttctgcca ttctaagaaa     2160

aagtgtgacg agctggccgc tgcactgcga ggaatgggac tgaatgctgt ggcatactat     2220

agaggactgg atgtctccat tatcccagca cagggcgacg tggtcgtggt cgcaaccgat     2280

gccctgatga ccggatacac aggcgacttc gatagcgtga ttgactgtaa cgtggccgtc     2340

acacaggctg tggacttcag cctggacccc accttcacca tcaccacaca gacagtgccc     2400

caggatgccg tcagtcgatc acagaggcgc ggaagaactg gcagagggag gcagggcaca     2460

tacagatacg tgagcactgg cgagcgcgct agtgggatgt ttgactcagt ggtcctgtgc     2520

gaatgttatg acgccggagc cgcttggtac gatctgactc ctgccgagac taccgtgcgc     2580

ctgcgagctt atttcaatac ccctggactg ccagtgtgcc aggaccacct ggagttctgg     2640

gaagccgtgt tcaccgggct gacccacatc gatgcacatt tcctgtccca gaccaaacag     2700

gcaggagaga actttgccta tctggtggct taccaggcaa cagtctgcgc cagggctaag     2760

gcaccacccc cttcttggga cgccatgtgg aaatgtctgg ctcggctgaa gcccactctg     2820

gccggaccta ccccactgct gtatagactg gggcctatta ccaatgaagt gactctgacc     2880

cacccaggca caaagtacat cgctacttgt atgcaggcag atctggaagt gatgacatct     2940

acttgggtcc tggctggagg ggtgctggca gccgtcgctg catactgcct ggccacaggg     3000

tgcgtgagca ttatcggaag gctgcatgtg aaccagcgcg tcgtcgtcgc ccccgataaa     3060

gaggtgctgt atgaggcatt tgatgagatg gaggagtgct ga                        3102


<210>  10
<211>  4895
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Propol II expression plasmid

<400>  10
ctagagagct tggcccattg catacgttgt atccatatca taatatgtac atttatattg       60

gctcatgtcc aacattaccg ccatgttgac attgattatt gactagttat taatagtaat      120

caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg      180

taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt      240

atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac      300

ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg      360

acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact      420

ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt      480

ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc      540

ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc      600

gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata      660

taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg      720

acctccatag aagacaccgg gaccgatcca gcctccggtc gaccgatcct gagaacttca      780

gggtgagttt ggggaccctt gattgttctt tctttttcgc tattgtaaaa ttcatgttat      840

atggaggggg caaagttttc agggtgttgt ttagaatggg aagatgtccc ttgtatcacc      900

atggaccctc atgataattt tgtttctttc actttctact ctgttgacaa ccattgtctc      960

ctcttatttt cttttcattt tcttgtaact ttttcgttaa actttagctt gcatttgtaa     1020

cgaattttta aattcacttt tgtttatttg tcagattgta agtactttct ctaatcactt     1080

ttttttcaag gcaatcaggg tatattatat tgtacttcag cacagtttta gagaacaatt     1140

gttataatta aatgataagg tagaatattt ctgcatataa attctggctg gcgtggaaat     1200

attcttattg gtagaaacaa ctacatcctg gtcatcatcc tgcctttctc tttatggtta     1260

caatgatata cactgtttga gatgaggata aaatactctg agtccaaacc gggcccctct     1320

gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt     1380

gtgctgtctc atcattttgg caaagaattc ctcgagcgta cgcctagggg atccagcgct     1440

atttaaatgc tagcatgcat gttaaccctg caggggtacc gcggccgcaa gcttagatcc     1500

gtcgaggaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc     1560

aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac     1620

atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca     1680

atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat     1740

ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct     1800

gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc     1860

cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt     1920

gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt     1980

ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc     2040

gacggatcgg ccgcaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc     2100

gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta     2160

atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa     2220

cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat     2280

tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg     2340

agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc     2400

aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt     2460

gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag     2520

tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc     2580

cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc     2640

ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt     2700

cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt     2760

atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc     2820

agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa     2880

gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa     2940

gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg     3000

tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga     3060

agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg     3120

gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg     3180

aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt     3240

aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact     3300

ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat     3360

gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg     3420

aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg     3480

ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat     3540

tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc     3600

ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg gttagctcct     3660

tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg     3720

cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg     3780

agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg     3840

cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa     3900

aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt     3960

aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt     4020

gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt     4080

gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca     4140

tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat     4200

ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa     4260

tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa     4320

atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact     4380

attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc     4440

actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa     4500

tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc     4560

gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt     4620

cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca     4680

ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt     4740

acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt     4800

ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata     4860

gggcgaattg gagctccacc gcggtggcgg ccgct                                4895


