                         SEQUENCE LISTING

<110>  Novo Nordisk A/S
 
<120>  Mating factor alpha pro-peptide variants

<130>  130089WO01

<160>  44    

<170>  PatentIn version 3.5

<210>  1
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  MFalpha propeptide variant subsequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Amino acid residue which is F, L, I or V.

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Amino acid residue which is  L, I, V or M.

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Amino acid residue which is  A, G, S, E, Q, Y, F, W, R, K, H, L, 
       I, V or M.

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Amino acid residue which is  S, Y, F, W, L, I, V or M.

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Amino acid residue which is  Y, W, L, I, V, M or S.

<400>  1

Xaa Xaa Xaa Xaa Xaa 
1               5   


<210>  2
<211>  85
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  2

Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      


Ala Leu Ala Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln 
            20                  25                  30          


Ile Pro Ala Glu Ala Val Ile Gly Tyr Leu Asp Leu Glu Gly Asp Phe 
        35                  40                  45              


Asp Val Ala Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu 
    50                  55                  60                  


Phe Ile Asn Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val 
65                  70                  75                  80  


Ser Leu Asp Lys Arg 
                85  


<210>  3
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  VIGYL sequence

<400>  3

Val Ile Gly Tyr Leu 
1               5   


<210>  4
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  VIGYS sequence

<400>  4

Val Ile Gly Tyr Ser 
1               5   


<210>  5
<211>  31
<212>  PRT
<213>  Homo sapiens

<400>  5

His Ala Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Gly Arg Gly 
            20                  25                  30      


<210>  6
<211>  31
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Analogue of GLP-1(7-37)

<400>  6

His Ala Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
            20                  25                  30      


<210>  7
<211>  29
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Analogue of GLP-1(9-37)

<400>  7

Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly Gln Ala 
1               5                   10                  15      


Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
            20                  25                  


<210>  8
<211>  66
<212>  PRT
<213>  Artificial sequence

<220>
<223>  MFalpha propeptide variant L42S-MFa(20-85)

<400>  8

Ala Pro Val Asn Thr Thr Thr Glu Asp Glu Thr Ala Gln Ile Pro Ala 
1               5                   10                  15      


Glu Ala Val Ile Gly Tyr Ser Asp Leu Glu Gly Asp Phe Asp Val Ala 
            20                  25                  30          


Val Leu Pro Phe Ser Asn Ser Thr Asn Asn Gly Leu Leu Phe Ile Asn 
        35                  40                  45              


Thr Thr Ile Ala Ser Ile Ala Ala Lys Glu Glu Gly Val Ser Leu Asp 
    50                  55                  60                  


Lys Arg 
65      


<210>  9
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 1

<400>  9

Glu Glu Ala Glu Lys 
1               5   


<210>  10
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 2

<400>  10

Glu Glu Ala His Lys 
1               5   


<210>  11
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 3

<400>  11

Glu Glu Ala Glu Ala His Lys 
1               5           


<210>  12
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 4

<400>  12

Glu Glu Ala Glu Ala Glu Ala His Lys 
1               5                   


<210>  13
<211>  5
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 5

<400>  13

Glu Glu Gly His Lys 
1               5   


<210>  14
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 6

<400>  14

Glu Glu Gly Glu Pro Lys 
1               5       


<210>  15
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 7

<400>  15

Glu Glu Ala His Glu Leu Lys 
1               5           


<210>  16
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 8

<400>  16

Glu Glu Ala His Glu Val Lys 
1               5           


<210>  17
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 9

<400>  17

Glu Glu Ala His Glu Met Lys 
1               5           


<210>  18
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 10

<400>  18

Glu Glu Ala His Glu Phe Lys 
1               5           


<210>  19
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 11

<400>  19

Glu Glu Ala His Glu Tyr Lys 
1               5           


<210>  20
<211>  15
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 12

<400>  20

Glu Glu Ala His Glu Trp Lys Glu Glu Gly Asn Thr Thr Pro Lys 
1               5                   10                  15  


<210>  21
<211>  11
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 13

<400>  21

Glu Glu Leu Asp Ala Arg Leu Glu Ala Leu Lys 
1               5                   10      


<210>  22
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 14

<400>  22

Gln Pro Met Tyr Lys Arg 
1               5       


<210>  23
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 15

<400>  23

Gly Gln Pro Met Tyr Lys 
1               5       


<210>  24
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 16

<400>  24

Pro Gly Gln Pro Met Tyr 
1               5       


<210>  25
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 17

<400>  25

Lys Pro Gly Gln Pro Met 
1               5       


<210>  26
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 18

<400>  26

Leu Lys Pro Gly Gln Pro 
1               5       


<210>  27
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 19

<400>  27

Gln Leu Lys Pro Gly Gln 
1               5       


<210>  28
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 20

<400>  28

Leu Gln Leu Lys Pro Gly 
1               5       


<210>  29
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 21

<400>  29

Trp Leu Gln Leu Lys Pro 
1               5       


<210>  30
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 22

<400>  30

His Trp Leu Gln Leu Lys 
1               5       


<210>  31
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 23

<400>  31

Trp His Trp Leu Gln Leu 
1               5       


<210>  32
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 24

<400>  32

Ala Trp His Trp Leu Gln 
1               5       


<210>  33
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 25

<400>  33

Glu Ala Trp His Trp Leu 
1               5       


<210>  34
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 26

<400>  34

Ala Glu Ala Trp His Trp 
1               5       


<210>  35
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 27

<400>  35

Glu Ala Glu Ala Trp His 
1               5       


<210>  36
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 28

<400>  36

Asp Val Lys Pro Gly Gln Pro Leu Ala 
1               5                   


<210>  37
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 29

<400>  37

Asp Val Lys Pro Gly Gln Pro Glu Tyr 
1               5                   


<210>  38
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 30

<400>  38

Asp Val Lys Pro Gly Glu Pro Leu Tyr 
1               5                   


<210>  39
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 31

<400>  39

Asp Val Lys Pro Gly Gln Pro Leu Tyr 
1               5                   


<210>  40
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 32

<400>  40

Asp Val Lys Pro Gly Gln Pro Leu Glu 
1               5                   


<210>  41
<211>  9
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 33

<400>  41

Asp Val Lys Pro Gly Gln Pro Met Tyr 
1               5                   


<210>  42
<211>  14
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extension 34

<400>  42

Asp Val Lys Pro Gly Gln Pro Met Tyr Asp Asp Asp Asp Lys 
1               5                   10                  


<210>  43
<211>  29
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Analogue of GLP-1(9-37)

<400>  43

Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly Gln Ala 
1               5                   10                  15      


Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Lys 
            20                  25                  


<210>  44
<211>  45
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Extended analogue of GLP-1(7-37)

<400>  44

Asp Val Lys Pro Gly Gln Pro Met Tyr Asp Asp Asp Asp Lys His Ala 
1               5                   10                  15      


Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly Gln Ala 
            20                  25                  30          


Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
        35                  40                  45  


