                         SEQUENCE LISTING

<110>  Merz Pharma GmbH & Co.KGaA
 
<120>  Gangliosides for standardizing and increasing the sensitivity of cells to Botulinum neurotoxins in in vitro test systems  

<130>  MP11612pc

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  3891
<212>  DNA
<213>  Clostridium botulinum

<400>  1
atgccatttg ttaataaaca atttaattat aaagatcctg taaatggtgt tgatattgct       60

tatataaaaa ttccaaatgc aggacaaatg caaccagtaa aagcttttaa aattcataat      120

aaaatatggg ttattccaga aagagataca tttacaaatc ctgaagaagg agatttaaat      180

ccaccaccag aagcaaaaca agttccagtt tcatattatg attcaacata tttaagtaca      240

gataatgaaa aagataatta tttaaaggga gttacaaaat tatttgagag aatttattca      300

actgatcttg gaagaatgtt gttaacatca atagtaaggg gaataccatt ttggggtgga      360

agtacaatag atacagaatt aaaagttatt gatactaatt gtattaatgt gatacaacca      420

gatggtagtt atagatcaga agaacttaat ctagtaataa taggaccctc agctgatatt      480

atacagtttg aatgtaaaag ctttggacat gaagttttga atcttacgcg aaatggttat      540

ggctctactc aatacattag atttagccca gattttacat ttggttttga ggagtcactt      600

gaagttgata caaatcctct tttaggtgca ggcaaatttg ctacagatcc agcagtaaca      660

ttagcacatg aacttataca tgctggacat agattatatg gaatagcaat taatccaaat      720

agggttttta aagtaaatac taatgcctat tatgaaatga gtgggttaga agtaagcttt      780

gaggaactta gaacatttgg gggacatgat gcaaagttta tagatagttt acaggaaaac      840

gaatttcgtc tatattatta taataagttt aaagatatag caagtacact taataaagct      900

aaatcaatag taggtactac tgcttcatta cagtatatga aaaatgtttt taaagagaaa      960

tatctcctat ctgaagatac atctggaaaa ttttcggtag ataaattaaa atttgataag     1020

ttatacaaaa tgttaacaga gatttacaca gaggataatt ttgttaagtt ttttaaagta     1080

cttaacagaa aaacatattt gaattttgat aaagccgtat ttaagataaa tatagtacct     1140

aaggtaaatt acacaatata tgatggattt aatttaagaa atacaaattt agcagcaaac     1200

tttaatggtc aaaatacaga aattaataat atgaatttta ctaaactaaa aaattttact     1260

ggattgtttg aattttataa gttgctatgt gtaagaggga taataacttc taaaactaaa     1320

tcattagata aaggatacaa taaggcatta aatgatttat gtatcaaagt taataattgg     1380

gacttgtttt ttagtccttc agaagataat tttactaatg atctaaataa aggagaagaa     1440

attacatctg atactaatat agaagcagca gaagaaaata ttagtttaga tttaatacaa     1500

caatattatt taacctttaa ttttgataat gaacctgaaa atatttcaat agaaaatctt     1560

tcaagtgaca ttataggcca attagaactt atgcctaata tagaaagatt tcctaatgga     1620

aaaaagtatg agttagataa atatactatg ttccattatc ttcgtgctca agaatttgaa     1680

catggtaaat ctaggattgc tttaacaaat tctgttaacg aagcattatt aaatcctagt     1740

cgtgtttata catttttttc ttcagactat gtaaagaaag ttaataaagc tacggaggca     1800

gctatgtttt taggctgggt agaacaatta gtatatgatt ttaccgatga aactagcgaa     1860

gtaagtacta cggataaaat tgcggatata actataatta ttccatatat aggacctgct     1920

ttaaatatag gtaatatgtt atataaagat gattttgtag gtgctttaat attttcagga     1980

gctgttattc tgttagaatt tataccagag attgcaatac ctgtattagg tacttttgca     2040

cttgtatcat atattgcgaa taaggttcta accgttcaaa caatagataa tgctttaagt     2100

aaaagaaatg aaaaatggga tgaggtctat aaatatatag taacaaattg gttagcaaag     2160

gttaatacac agattgatct aataagaaaa aaaatgaaag aagctttaga aaatcaagca     2220

gaagcaacaa aggctataat aaactatcag tataatcaat atactgagga agagaaaaat     2280

aatattaatt ttaatattga tgatttaagt tcgaaactta atgagtctat aaataaagct     2340

atgattaata taaataaatt tttgaatcaa tgctctgttt catatttaat gaattctatg     2400

atcccttatg gtgttaaacg gttagaagat tttgatgcta gtcttaaaga tgcattatta     2460

aagtatatat atgataatag aggaacttta attggtcaag tagatagatt aaaagataaa     2520

gttaataata cacttagtac agatatacct tttcagcttt ccaaatacgt agataatcaa     2580

agattattat ctacatttac tgaatatatt aagaatatta ttaatacttc tatattgaat     2640

ttaagatatg aaagtaatca tttaatagac ttatctaggt atgcatcaaa aataaatatt     2700

ggtagtaaag taaattttga tccaatagat aaaaatcaaa ttcaattatt taatttagaa     2760

agtagtaaaa ttgaggtaat tttaaaaaat gctattgtat ataatagtat gtatgaaaat     2820

tttagtacta gcttttggat aagaattcct aagtatttta acagtataag tctaaataat     2880

gaatatacaa taataaattg tatggaaaat aattcaggat ggaaagtatc acttaattat     2940

ggtgaaataa tctggacttt acaggatact caggaaataa aacaaagagt agtttttaaa     3000

tacagtcaaa tgattaatat atcagattat ataaacagat ggatttttgt aactatcact     3060

aataatagat taaataactc taaaatttat ataaatggaa gattaataga tcaaaaacca     3120

atttcaaatt taggtaatat tcatgctagt aataatataa tgtttaaatt agatggttgt     3180

agagatacac atagatatat ttggataaaa tattttaatc tttttgataa ggaattaaat     3240

gaaaaagaaa tcaaagattt atatgataat caatcaaatt caggtatttt aaaagacttt     3300

tggggtgatt atttacaata tgataaacca tactatatgt taaatttata tgatccaaat     3360

aaatatgtcg atgtaaataa tgtaggtatt agaggttata tgtatcttaa agggcctaga     3420

ggtagcgtaa tgactacaaa catttattta aattcaagtt tgtatagggg gacaaaattt     3480

attataaaaa aatatgcttc tggaaataaa gataatattg ttagaaataa tgatcgtgta     3540

tatattaatg tagtagttaa aaataaagaa tataggttag ctactaatgc atcacaggca     3600

ggcgtagaaa aaatactaag tgcattagaa atacctgatg taggaaatct aagtcaagta     3660

gtagtaatga agtcaaaaaa tgatcaagga ataacaaata aatgcaaaat gaatttacaa     3720

gataataatg ggaatgatat aggctttata ggatttcatc agtttaataa tatagctaaa     3780

ctagtagcaa gtaattggta taatagacaa atagaaagat ctagtaggac tttgggttgc     3840

tcatgggaat ttattcctgt agatgatgga tggggagaaa ggccactgta a              3891


<210>  2
<211>  1296
<212>  PRT
<213>  Clostridium botulinum

<400>  2

Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      


Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          


Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              


Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  


Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  


Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      


Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         


Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             


Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 


Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 


Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     


Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         


Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             


Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 


Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 


Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     


Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         


Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             


Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 


Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 


Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     


Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         


Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             


Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 


Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 


Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     


Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         


Gly Ile Ile Thr Ser Lys Thr Lys Ser Leu Asp Lys Gly Tyr Asn Lys 
        435                 440                 445             


Ala Leu Asn Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe 
    450                 455                 460                 


Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu 
465                 470                 475                 480 


Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu 
                485                 490                 495     


Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro 
            500                 505                 510         


Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu 
        515                 520                 525             


Glu Leu Met Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu 
    530                 535                 540                 


Leu Asp Lys Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu 
545                 550                 555                 560 


His Gly Lys Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu 
                565                 570                 575     


Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys 
            580                 585                 590         


Lys Val Asn Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu 
        595                 600                 605             


Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr 
    610                 615                 620                 


Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala 
625                 630                 635                 640 


Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu 
                645                 650                 655     


Ile Phe Ser Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala 
            660                 665                 670         


Ile Pro Val Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys 
        675                 680                 685             


Val Leu Thr Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu 
    690                 695                 700                 


Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys 
705                 710                 715                 720 


Val Asn Thr Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu 
                725                 730                 735     


Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn 
            740                 745                 750         


Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp 
        755                 760                 765             


Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile 
    770                 775                 780                 


Asn Lys Phe Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met 
785                 790                 795                 800 


Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys 
                805                 810                 815     


Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly 
            820                 825                 830         


Gln Val Asp Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp 
        835                 840                 845             


Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser 
    850                 855                 860                 


Thr Phe Thr Glu Tyr Ile Lys Asn Ile Ile Asn Thr Ser Ile Leu Asn 
865                 870                 875                 880 


Leu Arg Tyr Glu Ser Asn His Leu Ile Asp Leu Ser Arg Tyr Ala Ser 
                885                 890                 895     


Lys Ile Asn Ile Gly Ser Lys Val Asn Phe Asp Pro Ile Asp Lys Asn 
            900                 905                 910         


Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser Lys Ile Glu Val Ile Leu 
        915                 920                 925             


Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr Glu Asn Phe Ser Thr Ser 
    930                 935                 940                 


Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn Ser Ile Ser Leu Asn Asn 
945                 950                 955                 960 


Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn Asn Ser Gly Trp Lys Val 
                965                 970                 975     


Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr Leu Gln Asp Thr Gln Glu 
            980                 985                 990         


Ile Lys Gln Arg Val Val Phe Lys  Tyr Ser Gln Met Ile  Asn Ile Ser 
        995                 1000                 1005             


Asp Tyr  Ile Asn Arg Trp Ile  Phe Val Thr Ile Thr  Asn Asn Arg 
    1010                 1015                 1020             


Leu Asn  Asn Ser Lys Ile Tyr  Ile Asn Gly Arg Leu  Ile Asp Gln 
    1025                 1030                 1035             


Lys Pro  Ile Ser Asn Leu Gly  Asn Ile His Ala Ser  Asn Asn Ile 
    1040                 1045                 1050             


Met Phe  Lys Leu Asp Gly Cys  Arg Asp Thr His Arg  Tyr Ile Trp 
    1055                 1060                 1065             


Ile Lys  Tyr Phe Asn Leu Phe  Asp Lys Glu Leu Asn  Glu Lys Glu 
    1070                 1075                 1080             


Ile Lys  Asp Leu Tyr Asp Asn  Gln Ser Asn Ser Gly  Ile Leu Lys 
    1085                 1090                 1095             


Asp Phe  Trp Gly Asp Tyr Leu  Gln Tyr Asp Lys Pro  Tyr Tyr Met 
    1100                 1105                 1110             


Leu Asn  Leu Tyr Asp Pro Asn  Lys Tyr Val Asp Val  Asn Asn Val 
    1115                 1120                 1125             


Gly Ile  Arg Gly Tyr Met Tyr  Leu Lys Gly Pro Arg  Gly Ser Val 
    1130                 1135                 1140             


Met Thr  Thr Asn Ile Tyr Leu  Asn Ser Ser Leu Tyr  Arg Gly Thr 
    1145                 1150                 1155             


Lys Phe  Ile Ile Lys Lys Tyr  Ala Ser Gly Asn Lys  Asp Asn Ile 
    1160                 1165                 1170             


Val Arg  Asn Asn Asp Arg Val  Tyr Ile Asn Val Val  Val Lys Asn 
    1175                 1180                 1185             


Lys Glu  Tyr Arg Leu Ala Thr  Asn Ala Ser Gln Ala  Gly Val Glu 
    1190                 1195                 1200             


Lys Ile  Leu Ser Ala Leu Glu  Ile Pro Asp Val Gly  Asn Leu Ser 
    1205                 1210                 1215             


Gln Val  Val Val Met Lys Ser  Lys Asn Asp Gln Gly  Ile Thr Asn 
    1220                 1225                 1230             


Lys Cys  Lys Met Asn Leu Gln  Asp Asn Asn Gly Asn  Asp Ile Gly 
    1235                 1240                 1245             


Phe Ile  Gly Phe His Gln Phe  Asn Asn Ile Ala Lys  Leu Val Ala 
    1250                 1255                 1260             


Ser Asn  Trp Tyr Asn Arg Gln  Ile Glu Arg Ser Ser  Arg Thr Leu 
    1265                 1270                 1275             


Gly Cys  Ser Trp Glu Phe Ile  Pro Val Asp Asp Gly  Trp Gly Glu 
    1280                 1285                 1290             


Arg Pro  Leu 
    1295     


<210>  3
<211>  3876
<212>  DNA
<213>  Clostridium botulinum

<400>  3
atgccagtta caataaataa ttttaattat aatgatccta ttgataataa taatattatt       60

atgatggagc ctccatttgc gagaggtacg gggagatatt ataaagcttt taaaatcaca      120

gatcgtattt ggataatacc ggaaagatat acttttggat ataaacctga ggattttaat      180

aaaagttccg gtatttttaa tagagatgtt tgtgaatatt atgatccaga ttacttaaat      240

actaatgata aaaagaatat atttttacaa acaatgatca agttatttaa tagaatcaaa      300

tcaaaaccat tgggtgaaaa gttattagag atgattataa atggtatacc ttatcttgga      360

gatagacgtg ttccactcga agagtttaac acaaacattg ctagtgtaac tgttaataaa      420

ttaatcagta atccaggaga agtggagcga aaaaaaggta ttttcgcaaa tttaataata      480

tttggacctg ggccagtttt aaatgaaaat gagactatag atataggtat acaaaatcat      540

tttgcatcaa gggaaggctt cgggggtata atgcaaatga agttttgccc agaatatgta      600

agcgtattta ataatgttca agaaaacaaa ggcgcaagta tatttaatag acgtggatat      660

ttttcagatc cagccttgat attaatgcat gaacttatac atgttttaca tggattatat      720

ggcattaaag tagatgattt accaattgta ccaaatgaaa aaaaattttt tatgcaatct      780

acagatgcta tacaggcaga agaactatat acatttggag gacaagatcc cagcatcata      840

actccttcta cggataaaag tatctatgat aaagttttgc aaaattttag agggatagtt      900

gatagactta acaaggtttt agtttgcata tcagatccta acattaatat taatatatat      960

aaaaataaat ttaaagataa atataaattc gttgaagatt ctgagggaaa atatagtata     1020

gatgtagaaa gttttgataa attatataaa agcttaatgt ttggttttac agaaactaat     1080

atagcagaaa attataaaat aaaaactaga gcttcttatt ttagtgattc cttaccacca     1140

gtaaaaataa aaaatttatt agataatgaa atctatacta tagaggaagg gtttaatata     1200

tctgataaag atatggaaaa agaatataga ggtcagaata aagctataaa taaacaagct     1260

tatgaagaaa ttagcaagga gcatttggct gtatataaga tacaaatgtg taaaagtgtt     1320

aaagctccag gaatatgtat tgatgttgat aatgaagatt tgttctttat agctgataaa     1380

aatagttttt cagatgattt atctaaaaac gaaagaatag aatataatac acagagtaat     1440

tatatagaaa atgacttccc tataaatgaa ttaattttag atactgattt aataagtaaa     1500

atagaattac caagtgaaaa tacagaatca cttactgatt ttaatgtaga tgttccagta     1560

tatgaaaaac aacccgctat aaaaaaaatt tttacagatg aaaataccat ctttcaatat     1620

ttatactctc agacatttcc tctagatata agagatataa gtttaacatc ttcatttgat     1680

gatgcattat tattttctaa caaagtttat tcattttttt ctatggatta tattaaaact     1740

gctaataaag tggtagaagc aggattattt gcaggttggg tgaaacagat agtaaatgat     1800

tttgtaatcg aagctaataa aagcaatact atggataaaa ttgcagatat atctctaatt     1860

gttccttata taggattagc tttaaatgta ggaaatgaaa cagctaaagg aaattttgaa     1920

aatgcttttg agattgcagg agccagtatt ctactagaat ttataccaga acttttaata     1980

cctgtagttg gagccttttt attagaatca tatattgaca ataaaaataa aattattaaa     2040

acaatagata atgctttaac taaaagaaat gaaaaatgga gtgatatgta cggattaata     2100

gtagcgcaat ggctctcaac agttaatact caattttata caataaaaga gggaatgtat     2160

aaggctttaa attatcaagc acaagcattg gaagaaataa taaaatacag atataatata     2220

tattctgaaa aagaaaagtc aaatattaac atcgatttta atgatataaa ttctaaactt     2280

aatgagggta ttaaccaagc tatagataat ataaataatt ttataaatgg atgttctgta     2340

tcatatttaa tgaaaaaaat gattccatta gctgtagaaa aattactaga ctttgataat     2400

actctcaaaa aaaatttgtt aaattatata gatgaaaata aattatattt gattggaagt     2460

gcagaatatg aaaaatcaaa agtaaataaa tacttgaaaa ccattatgcc gtttgatctt     2520

tcaatatata ccaatgatac aatactaata gaaatgttta ataaatataa tagcgaaatt     2580

ttaaataata ttatcttaaa tttaagatat aaggataata atttaataga tttatcagga     2640

tatggggcaa aggtagaggt atatgatgga gtcgagctta atgataaaaa tcaatttaaa     2700

ttaactagtt cagcaaatag taagattaga gtgactcaaa atcagaatat catatttaat     2760

agtgtgttcc ttgattttag cgttagcttt tggataagaa tacctaaata taagaatgat     2820

ggtatacaaa attatattca taatgaatat acaataatta attgtatgaa aaataattcg     2880

ggctggaaaa tatctattag gggtaatagg ataatatgga ctttaattga tataaatgga     2940

aaaaccaaat cggtattttt tgaatataac ataagagaag atatatcaga gtatataaat     3000

agatggtttt ttgtaactat tactaataat ttgaataacg ctaaaattta tattaatggt     3060

aagctagaat caaatacaga tattaaagat ataagagaag ttattgctaa tggtgaaata     3120

atatttaaat tagatggtga tatagataga acacaattta tttggatgaa atatttcagt     3180

atttttaata cggaattaag tcaatcaaat attgaagaaa gatataaaat tcaatcatat     3240

agcgaatatt taaaagattt ttggggaaat cctttaatgt acaataaaga atattatatg     3300

tttaatgcgg ggaataaaaa ttcatatatt aaactaaaga aagattcacc tgtaggtgaa     3360

attttaacac gtagcaaata taatcaaaat tctaaatata taaattatag agatttatat     3420

attggagaaa aatttattat aagaagaaag tcaaattctc aatctataaa tgatgatata     3480

gttagaaaag aagattatat atatctagat ttttttaatt taaatcaaga gtggagagta     3540

tatacctata aatattttaa gaaagaggaa gaaaaattgt ttttagctcc tataagtgat     3600

tctgatgagt tttacaatac tatacaaata aaagaatatg atgaacagcc aacatatagt     3660

tgtcagttgc tttttaaaaa agatgaagaa agtactgatg agataggatt gattggtatt     3720

catcgtttct acgaatctgg aattgtattt gaagagtata aagattattt ttgtataagt     3780

aaatggtact taaaagaggt aaaaaggaaa ccatataatt taaaattggg atgtaattgg     3840

cagtttattc ctaaagatga agggtggact gaataa                               3876


<210>  4
<211>  1291
<212>  PRT
<213>  Clostridium botulinum

<400>  4

Met Pro Val Thr Ile Asn Asn Phe Asn Tyr Asn Asp Pro Ile Asp Asn 
1               5                   10                  15      


Asn Asn Ile Ile Met Met Glu Pro Pro Phe Ala Arg Gly Thr Gly Arg 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Lys Ile Thr Asp Arg Ile Trp Ile Ile Pro Glu 
        35                  40                  45              


Arg Tyr Thr Phe Gly Tyr Lys Pro Glu Asp Phe Asn Lys Ser Ser Gly 
    50                  55                  60                  


Ile Phe Asn Arg Asp Val Cys Glu Tyr Tyr Asp Pro Asp Tyr Leu Asn 
65                  70                  75                  80  


Thr Asn Asp Lys Lys Asn Ile Phe Leu Gln Thr Met Ile Lys Leu Phe 
                85                  90                  95      


Asn Arg Ile Lys Ser Lys Pro Leu Gly Glu Lys Leu Leu Glu Met Ile 
            100                 105                 110         


Ile Asn Gly Ile Pro Tyr Leu Gly Asp Arg Arg Val Pro Leu Glu Glu 
        115                 120                 125             


Phe Asn Thr Asn Ile Ala Ser Val Thr Val Asn Lys Leu Ile Ser Asn 
    130                 135                 140                 


Pro Gly Glu Val Glu Arg Lys Lys Gly Ile Phe Ala Asn Leu Ile Ile 
145                 150                 155                 160 


Phe Gly Pro Gly Pro Val Leu Asn Glu Asn Glu Thr Ile Asp Ile Gly 
                165                 170                 175     


Ile Gln Asn His Phe Ala Ser Arg Glu Gly Phe Gly Gly Ile Met Gln 
            180                 185                 190         


Met Lys Phe Cys Pro Glu Tyr Val Ser Val Phe Asn Asn Val Gln Glu 
        195                 200                 205             


Asn Lys Gly Ala Ser Ile Phe Asn Arg Arg Gly Tyr Phe Ser Asp Pro 
    210                 215                 220                 


Ala Leu Ile Leu Met His Glu Leu Ile His Val Leu His Gly Leu Tyr 
225                 230                 235                 240 


Gly Ile Lys Val Asp Asp Leu Pro Ile Val Pro Asn Glu Lys Lys Phe 
                245                 250                 255     


Phe Met Gln Ser Thr Asp Ala Ile Gln Ala Glu Glu Leu Tyr Thr Phe 
            260                 265                 270         


Gly Gly Gln Asp Pro Ser Ile Ile Thr Pro Ser Thr Asp Lys Ser Ile 
        275                 280                 285             


Tyr Asp Lys Val Leu Gln Asn Phe Arg Gly Ile Val Asp Arg Leu Asn 
    290                 295                 300                 


Lys Val Leu Val Cys Ile Ser Asp Pro Asn Ile Asn Ile Asn Ile Tyr 
305                 310                 315                 320 


Lys Asn Lys Phe Lys Asp Lys Tyr Lys Phe Val Glu Asp Ser Glu Gly 
                325                 330                 335     


Lys Tyr Ser Ile Asp Val Glu Ser Phe Asp Lys Leu Tyr Lys Ser Leu 
            340                 345                 350         


Met Phe Gly Phe Thr Glu Thr Asn Ile Ala Glu Asn Tyr Lys Ile Lys 
        355                 360                 365             


Thr Arg Ala Ser Tyr Phe Ser Asp Ser Leu Pro Pro Val Lys Ile Lys 
    370                 375                 380                 


Asn Leu Leu Asp Asn Glu Ile Tyr Thr Ile Glu Glu Gly Phe Asn Ile 
385                 390                 395                 400 


Ser Asp Lys Asp Met Glu Lys Glu Tyr Arg Gly Gln Asn Lys Ala Ile 
                405                 410                 415     


Asn Lys Gln Ala Tyr Glu Glu Ile Ser Lys Glu His Leu Ala Val Tyr 
            420                 425                 430         


Lys Ile Gln Met Cys Lys Ser Val Lys Ala Pro Gly Ile Cys Ile Asp 
        435                 440                 445             


Val Asp Asn Glu Asp Leu Phe Phe Ile Ala Asp Lys Asn Ser Phe Ser 
    450                 455                 460                 


Asp Asp Leu Ser Lys Asn Glu Arg Ile Glu Tyr Asn Thr Gln Ser Asn 
465                 470                 475                 480 


Tyr Ile Glu Asn Asp Phe Pro Ile Asn Glu Leu Ile Leu Asp Thr Asp 
                485                 490                 495     


Leu Ile Ser Lys Ile Glu Leu Pro Ser Glu Asn Thr Glu Ser Leu Thr 
            500                 505                 510         


Asp Phe Asn Val Asp Val Pro Val Tyr Glu Lys Gln Pro Ala Ile Lys 
        515                 520                 525             


Lys Ile Phe Thr Asp Glu Asn Thr Ile Phe Gln Tyr Leu Tyr Ser Gln 
    530                 535                 540                 


Thr Phe Pro Leu Asp Ile Arg Asp Ile Ser Leu Thr Ser Ser Phe Asp 
545                 550                 555                 560 


Asp Ala Leu Leu Phe Ser Asn Lys Val Tyr Ser Phe Phe Ser Met Asp 
                565                 570                 575     


Tyr Ile Lys Thr Ala Asn Lys Val Val Glu Ala Gly Leu Phe Ala Gly 
            580                 585                 590         


Trp Val Lys Gln Ile Val Asn Asp Phe Val Ile Glu Ala Asn Lys Ser 
        595                 600                 605             


Asn Thr Met Asp Lys Ile Ala Asp Ile Ser Leu Ile Val Pro Tyr Ile 
    610                 615                 620                 


Gly Leu Ala Leu Asn Val Gly Asn Glu Thr Ala Lys Gly Asn Phe Glu 
625                 630                 635                 640 


Asn Ala Phe Glu Ile Ala Gly Ala Ser Ile Leu Leu Glu Phe Ile Pro 
                645                 650                 655     


Glu Leu Leu Ile Pro Val Val Gly Ala Phe Leu Leu Glu Ser Tyr Ile 
            660                 665                 670         


Asp Asn Lys Asn Lys Ile Ile Lys Thr Ile Asp Asn Ala Leu Thr Lys 
        675                 680                 685             


Arg Asn Glu Lys Trp Ser Asp Met Tyr Gly Leu Ile Val Ala Gln Trp 
    690                 695                 700                 


Leu Ser Thr Val Asn Thr Gln Phe Tyr Thr Ile Lys Glu Gly Met Tyr 
705                 710                 715                 720 


Lys Ala Leu Asn Tyr Gln Ala Gln Ala Leu Glu Glu Ile Ile Lys Tyr 
                725                 730                 735     


Arg Tyr Asn Ile Tyr Ser Glu Lys Glu Lys Ser Asn Ile Asn Ile Asp 
            740                 745                 750         


Phe Asn Asp Ile Asn Ser Lys Leu Asn Glu Gly Ile Asn Gln Ala Ile 
        755                 760                 765             


Asp Asn Ile Asn Asn Phe Ile Asn Gly Cys Ser Val Ser Tyr Leu Met 
    770                 775                 780                 


Lys Lys Met Ile Pro Leu Ala Val Glu Lys Leu Leu Asp Phe Asp Asn 
785                 790                 795                 800 


Thr Leu Lys Lys Asn Leu Leu Asn Tyr Ile Asp Glu Asn Lys Leu Tyr 
                805                 810                 815     


Leu Ile Gly Ser Ala Glu Tyr Glu Lys Ser Lys Val Asn Lys Tyr Leu 
            820                 825                 830         


Lys Thr Ile Met Pro Phe Asp Leu Ser Ile Tyr Thr Asn Asp Thr Ile 
        835                 840                 845             


Leu Ile Glu Met Phe Asn Lys Tyr Asn Ser Glu Ile Leu Asn Asn Ile 
    850                 855                 860                 


Ile Leu Asn Leu Arg Tyr Lys Asp Asn Asn Leu Ile Asp Leu Ser Gly 
865                 870                 875                 880 


Tyr Gly Ala Lys Val Glu Val Tyr Asp Gly Val Glu Leu Asn Asp Lys 
                885                 890                 895     


Asn Gln Phe Lys Leu Thr Ser Ser Ala Asn Ser Lys Ile Arg Val Thr 
            900                 905                 910         


Gln Asn Gln Asn Ile Ile Phe Asn Ser Val Phe Leu Asp Phe Ser Val 
        915                 920                 925             


Ser Phe Trp Ile Arg Ile Pro Lys Tyr Lys Asn Asp Gly Ile Gln Asn 
    930                 935                 940                 


Tyr Ile His Asn Glu Tyr Thr Ile Ile Asn Cys Met Lys Asn Asn Ser 
945                 950                 955                 960 


Gly Trp Lys Ile Ser Ile Arg Gly Asn Arg Ile Ile Trp Thr Leu Ile 
                965                 970                 975     


Asp Ile Asn Gly Lys Thr Lys Ser Val Phe Phe Glu Tyr Asn Ile Arg 
            980                 985                 990         


Glu Asp Ile Ser Glu Tyr Ile Asn  Arg Trp Phe Phe Val  Thr Ile Thr 
        995                 1000                 1005             


Asn Asn  Leu Asn Asn Ala Lys  Ile Tyr Ile Asn Gly  Lys Leu Glu 
    1010                 1015                 1020             


Ser Asn  Thr Asp Ile Lys Asp  Ile Arg Glu Val Ile  Ala Asn Gly 
    1025                 1030                 1035             


Glu Ile  Ile Phe Lys Leu Asp  Gly Asp Ile Asp Arg  Thr Gln Phe 
    1040                 1045                 1050             


Ile Trp  Met Lys Tyr Phe Ser  Ile Phe Asn Thr Glu  Leu Ser Gln 
    1055                 1060                 1065             


Ser Asn  Ile Glu Glu Arg Tyr  Lys Ile Gln Ser Tyr  Ser Glu Tyr 
    1070                 1075                 1080             


Leu Lys  Asp Phe Trp Gly Asn  Pro Leu Met Tyr Asn  Lys Glu Tyr 
    1085                 1090                 1095             


Tyr Met  Phe Asn Ala Gly Asn  Lys Asn Ser Tyr Ile  Lys Leu Lys 
    1100                 1105                 1110             


Lys Asp  Ser Pro Val Gly Glu  Ile Leu Thr Arg Ser  Lys Tyr Asn 
    1115                 1120                 1125             


Gln Asn  Ser Lys Tyr Ile Asn  Tyr Arg Asp Leu Tyr  Ile Gly Glu 
    1130                 1135                 1140             


Lys Phe  Ile Ile Arg Arg Lys  Ser Asn Ser Gln Ser  Ile Asn Asp 
    1145                 1150                 1155             


Asp Ile  Val Arg Lys Glu Asp  Tyr Ile Tyr Leu Asp  Phe Phe Asn 
    1160                 1165                 1170             


Leu Asn  Gln Glu Trp Arg Val  Tyr Thr Tyr Lys Tyr  Phe Lys Lys 
    1175                 1180                 1185             


Glu Glu  Glu Lys Leu Phe Leu  Ala Pro Ile Ser Asp  Ser Asp Glu 
    1190                 1195                 1200             


Phe Tyr  Asn Thr Ile Gln Ile  Lys Glu Tyr Asp Glu  Gln Pro Thr 
    1205                 1210                 1215             


Tyr Ser  Cys Gln Leu Leu Phe  Lys Lys Asp Glu Glu  Ser Thr Asp 
    1220                 1225                 1230             


Glu Ile  Gly Leu Ile Gly Ile  His Arg Phe Tyr Glu  Ser Gly Ile 
    1235                 1240                 1245             


Val Phe  Glu Glu Tyr Lys Asp  Tyr Phe Cys Ile Ser  Lys Trp Tyr 
    1250                 1255                 1260             


Leu Lys  Glu Val Lys Arg Lys  Pro Tyr Asn Leu Lys  Leu Gly Cys 
    1265                 1270                 1275             


Asn Trp  Gln Phe Ile Pro Lys  Asp Glu Gly Trp Thr  Glu 
    1280                 1285                 1290     


<210>  5
<211>  3843
<212>  DNA
<213>  Clostridium botulinum

<400>  5
atgccaataa caattaacaa ctttaattat tcagatcctg ttgataataa aaatatttta       60

tatttagata ctcatttaaa tacattagct aatgagcctg aaaaagcctt tcgcattata      120

gggaatatat gggtaatacc cgatagattt tcaagagatt ctaatccaaa tttaaataaa      180

cctcctcgag ttacaagccc taaaagtggt tattatgatc ctaattattt gagtactgat      240

tctgaaaaag atacattttt aaaagaaatt ataaagttat ttaaaagaat taactctaga      300

gaaataggag aagaattaat atatagactt gcaacagaca taccctttcc tgggaataac      360

aatactccaa ttaatacttt tgattttgat gtagatttta acagtgttga tgttaaaact      420

agacaaggta acaactgggt taaaactggt agtataaatc ctagtgttat aataactgga      480

cctagagaaa acattataga cccagaaact tctacgttta aattaactaa caatactttt      540

gcggcacaag aaggatttgg tgctttatca ataatttcaa tatcacctag atttatgcta      600

acatatagta atgcaactaa taatgtagga gagggtagat tttctaagtc tgaattttgc      660

atggatccaa tactaatttt aatgcatgaa cttaatcatg caatgcataa tttatatgga      720

atagctatac caaatgatca aagaatttca tctgtaacta gtaatatttt ttattctcaa      780

tataaggtga aattagagta tgcagaaata tatgcatttg gaggtccaac tatagacctt      840

attcctaaaa gtgcaaggaa atattttgag gaaaaggcat tggattatta tagatccata      900

gctaaaagac ttaatagtat aactactgca aatccttcaa gctttaataa atatatagga      960

gaatataaac agaaacttat tagaaagtat agattcgtag tagaatcttc aggtgaagtt     1020

gcagtagatc gtaataagtt tgctgagtta tataaagaac ttacacaaat atttacagaa     1080

tttaactacg ctaaaatata taatgtacaa aataggaaaa tatatctttc aaatgtatat     1140

actccggtta cggcaaatat attagacgat aatgtttatg atatacaaaa tggatttaac     1200

atacctaaaa gtaatttaaa tgtactattt atgggtcaaa atttatctcg aaatccagca     1260

ttaagaaaag tcaatcctga aaatatgctt tatttattta caaaattttg ccataaagca     1320

atagatggta gatcattata taataaaaca ttagattgta gagagctttt agttaaaaat     1380

actgacttac cctttatagg tgatattagt gatatcaaaa ctgatatatt tttaagcaaa     1440

gatattaatg aagaaactga agttatagac tatccggaca atgtttcagt ggatcaagtt     1500

attctcagta agaatacctc agaacatgga caactagatt tattataccc tattattgaa     1560

ggtgagagtc aagtattacc gggagagaat caagtctttt atgataatag aactcaaaat     1620

gttgattatt tgaattctta ttattaccta gaatctcaaa aactaagtga taatgttgaa     1680

gattttactt ttacgacatc aattgaggaa gctttggata atagtggaaa agtatatact     1740

tactttccta aactagctga taaagtaaat acgggtgttc aaggtggttt atttttaatg     1800

tgggcaaatg atgtagttga agattttact acaaatattc taagaaaaga tacattagat     1860

aaaatatcag atgtatcagc tattattccc tatataggac ctgcattaaa tataagtaat     1920

tctgtaagaa ggggaaattt tactgaagca tttgcagtta ccggtgtaac tattttatta     1980

gaagcgtttc aagaatttac aatacctgca cttggtgcat ttgtgattta tagtaaggtt     2040

caagaaagaa acgagattat taaaactata gataattgtt tagaacaaag gattaaaaga     2100

tggaaagatt catatgaatg gatgatagga acgtggttat ccaggattac tactcaattt     2160

aataatataa gttatcaaat gtatgattct ttaaattatc aggcagatgc aatcaaagat     2220

aaaatagatt tagaatataa aaaatactca ggaagtgata aagaaaatat aaaaagtcaa     2280

gttgaaaatt taaaaaatag tttagatata aaaatctcgg aagcaatgaa taatataaat     2340

aaatttatac gagaatgttc tgtaacatac ttatttaaaa atatgctccc taaagtaatt     2400

gatgaattaa ataagtttga tttaaaaact aaaacagaat taattaatct tatagatagt     2460

cataatatta ttctagttgg tgaagtagat agattaaaag caaaagtaaa tgagagtttt     2520

gaaaatacaa taccctttaa tattttttca tatactaata attctttatt aaaagatata     2580

attaatgaat atttcaatag tattaatgat tcaaaaattt tgagcttaca aaacaaaaaa     2640

aatgctttag tggatacatc aggatataat gcagaagtga ggctagaagg tgatgttcaa     2700

gttaatacga tatatacaaa tgattttaaa ttaagtagtt caggagataa aattatagta     2760

aatttaaata ataatatttt atatagcgct atttatgaga actctagtgt tagtttttgg     2820

attaagatat ctaaagattt aactaattct cataatgaat atacaataat taatagtata     2880

aaacaaaatt ctgggtggaa attatgtatt aggaatggca atatagaatg gattttacaa     2940

gatattaata gaaagtataa aagtttaatt tttgattata gtgaatcatt aagtcataca     3000

ggatatacaa ataaatggtt ttttgttact ataactaata atataatggg gtatatgaaa     3060

ctttatataa atggagaatt aaagcagagt gaaagaattg aagatttaaa tgaggttaag     3120

ttagataaaa ccatagtatt tggaatagat gagaatatag atgagaatca gatgctttgg     3180

attagagatt ttaatatttt ttctaaagaa ttaagcaatg aagatattaa tattgtatat     3240

gagggacaaa tattaagaaa tgttattaaa gattattggg gaaatccttt gaagtttgat     3300

acagaatatt atattattaa tgataattat atagataggt atatagcacc taaaagtaat     3360

atacttgtac ttgttcagta tccagataga tctaaattat atactggaaa tcctattact     3420

attaaatcag tatctgataa gaatccttat agtagaattt taaatggaga taatataatg     3480

tttcatatgt tatataatag tgggaaatat atgataataa gagatactga tacaatatat     3540

gcaatagaag gaagagagtg ttcaaaaaat tgtgtatatg cattaaaatt acagagtaat     3600

ttaggtaatt atggtatagg tatatttagt ataaaaaata ttgtatctca aaataaatat     3660

tgtagtcaaa ttttctctag ttttatgaaa aatacaatgc ttctagcaga tatatataaa     3720

ccttggagat tttcttttga aaatgcatac acgccagttg cagtaactaa ttatgagaca     3780

aaactattat caacttcatc tttttggaaa tttatttcta gggatccagg atgggtagag     3840

taa                                                                   3843


<210>  6
<211>  1280
<212>  PRT
<213>  Clostridium botulinum

<400>  6

Met Pro Ile Thr Ile Asn Asn Phe Asn Tyr Ser Asp Pro Val Asp Asn 
1               5                   10                  15      


Lys Asn Ile Leu Tyr Leu Asp Thr His Leu Asn Thr Leu Ala Asn Glu 
            20                  25                  30          


Pro Glu Lys Ala Phe Arg Ile Ile Gly Asn Ile Trp Val Ile Pro Asp 
        35                  40                  45              


Arg Phe Ser Arg Asp Ser Asn Pro Asn Leu Asn Lys Pro Pro Arg Val 
    50                  55                  60                  


Thr Ser Pro Lys Ser Gly Tyr Tyr Asp Pro Asn Tyr Leu Ser Thr Asp 
65                  70                  75                  80  


Ser Glu Lys Asp Thr Phe Leu Lys Glu Ile Ile Lys Leu Phe Lys Arg 
                85                  90                  95      


Ile Asn Ser Arg Glu Ile Gly Glu Glu Leu Ile Tyr Arg Leu Ala Thr 
            100                 105                 110         


Asp Ile Pro Phe Pro Gly Asn Asn Asn Thr Pro Ile Asn Thr Phe Asp 
        115                 120                 125             


Phe Asp Val Asp Phe Asn Ser Val Asp Val Lys Thr Arg Gln Gly Asn 
    130                 135                 140                 


Asn Trp Val Lys Thr Gly Ser Ile Asn Pro Ser Val Ile Ile Thr Gly 
145                 150                 155                 160 


Pro Arg Glu Asn Ile Ile Asp Pro Glu Thr Ser Thr Phe Lys Leu Thr 
                165                 170                 175     


Asn Asn Thr Phe Ala Ala Gln Glu Gly Phe Gly Ala Leu Ser Ile Ile 
            180                 185                 190         


Ser Ile Ser Pro Arg Phe Met Leu Thr Tyr Ser Asn Ala Thr Asn Asn 
        195                 200                 205             


Val Gly Glu Gly Arg Phe Ser Lys Ser Glu Phe Cys Met Asp Pro Ile 
    210                 215                 220                 


Leu Ile Leu Met His Glu Leu Asn His Ala Met His Asn Leu Tyr Gly 
225                 230                 235                 240 


Ile Ala Ile Pro Asn Asp Gln Arg Ile Ser Ser Val Thr Ser Asn Ile 
                245                 250                 255     


Phe Tyr Ser Gln Tyr Lys Val Lys Leu Glu Tyr Ala Glu Ile Tyr Ala 
            260                 265                 270         


Phe Gly Gly Pro Thr Ile Asp Leu Ile Pro Lys Ser Ala Arg Lys Tyr 
        275                 280                 285             


Phe Glu Glu Lys Ala Leu Asp Tyr Tyr Arg Ser Ile Ala Lys Arg Leu 
    290                 295                 300                 


Asn Ser Ile Thr Thr Ala Asn Pro Ser Ser Phe Asn Lys Tyr Ile Gly 
305                 310                 315                 320 


Glu Tyr Lys Gln Lys Leu Ile Arg Lys Tyr Arg Phe Val Val Glu Ser 
                325                 330                 335     


Ser Gly Glu Val Ala Val Asp Arg Asn Lys Phe Ala Glu Leu Tyr Lys 
            340                 345                 350         


Glu Leu Thr Gln Ile Phe Thr Glu Phe Asn Tyr Ala Lys Ile Tyr Asn 
        355                 360                 365             


Val Gln Asn Arg Lys Ile Tyr Leu Ser Asn Val Tyr Thr Pro Val Thr 
    370                 375                 380                 


Ala Asn Ile Leu Asp Asp Asn Val Tyr Asp Ile Gln Asn Gly Phe Asn 
385                 390                 395                 400 


Ile Pro Lys Ser Asn Leu Asn Val Leu Phe Met Gly Gln Asn Leu Ser 
                405                 410                 415     


Arg Asn Pro Ala Leu Arg Lys Val Asn Pro Glu Asn Met Leu Tyr Leu 
            420                 425                 430         


Phe Thr Lys Phe Cys His Lys Ala Ile Asp Gly Arg Ser Leu Tyr Asn 
        435                 440                 445             


Lys Thr Leu Asp Cys Arg Glu Leu Leu Val Lys Asn Thr Asp Leu Pro 
    450                 455                 460                 


Phe Ile Gly Asp Ile Ser Asp Ile Lys Thr Asp Ile Phe Leu Ser Lys 
465                 470                 475                 480 


Asp Ile Asn Glu Glu Thr Glu Val Ile Asp Tyr Pro Asp Asn Val Ser 
                485                 490                 495     


Val Asp Gln Val Ile Leu Ser Lys Asn Thr Ser Glu His Gly Gln Leu 
            500                 505                 510         


Asp Leu Leu Tyr Pro Ile Ile Glu Gly Glu Ser Gln Val Leu Pro Gly 
        515                 520                 525             


Glu Asn Gln Val Phe Tyr Asp Asn Arg Thr Gln Asn Val Asp Tyr Leu 
    530                 535                 540                 


Asn Ser Tyr Tyr Tyr Leu Glu Ser Gln Lys Leu Ser Asp Asn Val Glu 
545                 550                 555                 560 


Asp Phe Thr Phe Thr Thr Ser Ile Glu Glu Ala Leu Asp Asn Ser Gly 
                565                 570                 575     


Lys Val Tyr Thr Tyr Phe Pro Lys Leu Ala Asp Lys Val Asn Thr Gly 
            580                 585                 590         


Val Gln Gly Gly Leu Phe Leu Met Trp Ala Asn Asp Val Val Glu Asp 
        595                 600                 605             


Phe Thr Thr Asn Ile Leu Arg Lys Asp Thr Leu Asp Lys Ile Ser Asp 
    610                 615                 620                 


Val Ser Ala Ile Ile Pro Tyr Ile Gly Pro Ala Leu Asn Ile Ser Asn 
625                 630                 635                 640 


Ser Val Arg Arg Gly Asn Phe Thr Glu Ala Phe Ala Val Thr Gly Val 
                645                 650                 655     


Thr Ile Leu Leu Glu Ala Phe Gln Glu Phe Thr Ile Pro Ala Leu Gly 
            660                 665                 670         


Ala Phe Val Ile Tyr Ser Lys Val Gln Glu Arg Asn Glu Ile Ile Lys 
        675                 680                 685             


Thr Ile Asp Asn Cys Leu Glu Gln Arg Ile Lys Arg Trp Lys Asp Ser 
    690                 695                 700                 


Tyr Glu Trp Met Ile Gly Thr Trp Leu Ser Arg Ile Thr Thr Gln Phe 
705                 710                 715                 720 


Asn Asn Ile Ser Tyr Gln Met Tyr Asp Ser Leu Asn Tyr Gln Ala Asp 
                725                 730                 735     


Ala Ile Lys Asp Lys Ile Asp Leu Glu Tyr Lys Lys Tyr Ser Gly Ser 
            740                 745                 750         


Asp Lys Glu Asn Ile Lys Ser Gln Val Glu Asn Leu Lys Asn Ser Leu 
        755                 760                 765             


Asp Ile Lys Ile Ser Glu Ala Met Asn Asn Ile Asn Lys Phe Ile Arg 
    770                 775                 780                 


Glu Cys Ser Val Thr Tyr Leu Phe Lys Asn Met Leu Pro Lys Val Ile 
785                 790                 795                 800 


Asp Glu Leu Asn Lys Phe Asp Leu Lys Thr Lys Thr Glu Leu Ile Asn 
                805                 810                 815     


Leu Ile Asp Ser His Asn Ile Ile Leu Val Gly Glu Val Asp Arg Leu 
            820                 825                 830         


Lys Ala Lys Val Asn Glu Ser Phe Glu Asn Thr Ile Pro Phe Asn Ile 
        835                 840                 845             


Phe Ser Tyr Thr Asn Asn Ser Leu Leu Lys Asp Ile Ile Asn Glu Tyr 
    850                 855                 860                 


Phe Asn Ser Ile Asn Asp Ser Lys Ile Leu Ser Leu Gln Asn Lys Lys 
865                 870                 875                 880 


Asn Ala Leu Val Asp Thr Ser Gly Tyr Asn Ala Glu Val Arg Leu Glu 
                885                 890                 895     


Gly Asp Val Gln Val Asn Thr Ile Tyr Thr Asn Asp Phe Lys Leu Ser 
            900                 905                 910         


Ser Ser Gly Asp Lys Ile Ile Val Asn Leu Asn Asn Asn Ile Leu Tyr 
        915                 920                 925             


Ser Ala Ile Tyr Glu Asn Ser Ser Val Ser Phe Trp Ile Lys Ile Ser 
    930                 935                 940                 


Lys Asp Leu Thr Asn Ser His Asn Glu Tyr Thr Ile Ile Asn Ser Ile 
945                 950                 955                 960 


Lys Gln Asn Ser Gly Trp Lys Leu Cys Ile Arg Asn Gly Asn Ile Glu 
                965                 970                 975     


Trp Ile Leu Gln Asp Ile Asn Arg Lys Tyr Lys Ser Leu Ile Phe Asp 
            980                 985                 990         


Tyr Ser Glu Ser Leu Ser His Thr  Gly Tyr Thr Asn Lys  Trp Phe Phe 
        995                 1000                 1005             


Val Thr  Ile Thr Asn Asn Ile  Met Gly Tyr Met Lys  Leu Tyr Ile 
    1010                 1015                 1020             


Asn Gly  Glu Leu Lys Gln Ser  Glu Arg Ile Glu Asp  Leu Asn Glu 
    1025                 1030                 1035             


Val Lys  Leu Asp Lys Thr Ile  Val Phe Gly Ile Asp  Glu Asn Ile 
    1040                 1045                 1050             


Asp Glu  Asn Gln Met Leu Trp  Ile Arg Asp Phe Asn  Ile Phe Ser 
    1055                 1060                 1065             


Lys Glu  Leu Ser Asn Glu Asp  Ile Asn Ile Val Tyr  Glu Gly Gln 
    1070                 1075                 1080             


Ile Leu  Arg Asn Val Ile Lys  Asp Tyr Trp Gly Asn  Pro Leu Lys 
    1085                 1090                 1095             


Phe Asp  Thr Glu Tyr Tyr Ile  Ile Asn Asp Asn Tyr  Ile Asp Arg 
    1100                 1105                 1110             


Tyr Ile  Ala Pro Lys Ser Asn  Ile Leu Val Leu Val  Gln Tyr Pro 
    1115                 1120                 1125             


Asp Arg  Ser Lys Leu Tyr Thr  Gly Asn Pro Ile Thr  Ile Lys Ser 
    1130                 1135                 1140             


Val Ser  Asp Lys Asn Pro Tyr  Ser Arg Ile Leu Asn  Gly Asp Asn 
    1145                 1150                 1155             


Ile Met  Phe His Met Leu Tyr  Asn Ser Gly Lys Tyr  Met Ile Ile 
    1160                 1165                 1170             


Arg Asp  Thr Asp Thr Ile Tyr  Ala Ile Glu Gly Arg  Glu Cys Ser 
    1175                 1180                 1185             


Lys Asn  Cys Val Tyr Ala Leu  Lys Leu Gln Ser Asn  Leu Gly Asn 
    1190                 1195                 1200             


Tyr Gly  Ile Gly Ile Phe Ser  Ile Lys Asn Ile Val  Ser Gln Asn 
    1205                 1210                 1215             


Lys Tyr  Cys Ser Gln Ile Phe  Ser Ser Phe Met Lys  Asn Thr Met 
    1220                 1225                 1230             


Leu Leu  Ala Asp Ile Tyr Lys  Pro Trp Arg Phe Ser  Phe Glu Asn 
    1235                 1240                 1245             


Ala Tyr  Thr Pro Val Ala Val  Thr Asn Tyr Glu Thr  Lys Leu Leu 
    1250                 1255                 1260             


Ser Thr  Ser Ser Phe Trp Lys  Phe Ile Ser Arg Asp  Pro Gly Trp 
    1265                 1270                 1275             


Val Glu  
    1280 

<210>  7
<211>  3858
<212>  DNA
<213>  Clostridium botulinum

<400>  7
atgacatggc cagtaaaaga ttttaattat agtgatcctg ttaatgacaa tgatatatta       60

tatttaagaa taccacaaaa taagttaatt actacacctg taaaagcttt tatgattact      120

caaaatattt gggtaatacc agaaagattt tcatcagata ctaatccaag tttaagtaaa      180

ccgcctagac ctacttcaaa gtatcaaagt tattatgatc ctagttattt atctactgat      240

gagcaaaaag atacattttt aaaagggatt ataaaattat ttaaaagaat taatgaaaga      300

gatataggaa aaaaattaat aaattattta gtagttggtt caccttttat gggagattca      360

agtacgcctg aagatacatt tgattttaca cgtcatacta ctaatattgc agttgaaaag      420

tttgaaaatg gtagttggaa agtaacaaat attataacac caagtgtatt gatatttgga      480

ccacttccta atatattaga ctatacagca tcccttacat tgcaaggaca acaatcaaat      540

ccatcatttg aagggtttgg aacattatct atactaaaag tagcacctga atttttgtta      600

acatttagtg atgtaacatc taatcaaagt tcagctgtat taggcaaatc tatattttgt      660

atggatccag taatagcttt aatgcatgag ttaacacatt ctttgcatca attgtatgga      720

ataaatatac catctgataa aaggattcgt ccacaagtta gcgagggatt tttttctcaa      780

gatggaccca acgtacaatt tgaggaatta tacacatttg gaggatcaga tgttgaaata      840

atacctcaaa ttgaaagatt acaattaaga gaaaaagcat taggtcacta taaagatata      900

gcgaaaagac ttaataatat taataaaact attccttcta gttggagtag taatatagat      960

aaatataaaa aaatattttc tgaaaagtat aattttgata aagataatac aggaaatttt     1020

gttgtaaata ttgataaatt caatagctta tattcagact tgactaatgt tatgtcagaa     1080

gttgtttatt cttcgcaata taatgttaaa aacaggactc attatttttc aaagcattat     1140

ctacctgtat ttgcaaatat attagatgat aatatttata ctataataaa cggttttaat     1200

ttaacaacta aaggttttaa tatagaaaat tcgggtcaga atatagaaag gaatcctgca     1260

ctacaaaaac ttagttcaga aagtgtagta gatttgttta caaaagtatg tttaagatta     1320

acaagaaata gtagagatga ttcaacatgt attcaagtta aaaataatac attaccttat     1380

gtagctgata aagatagcat ttcacaagaa atatttgaaa gtcaaattat tacagatgag     1440

actaatgtag aaaattattc agataatttt tcattagatg aatctatttt agatgcaaaa     1500

gtccctacta atcctgaagc agtagatcca ctgttaccca atgttaatat ggaaccttta     1560

aatgttccag gtgaagaaga agtattttat gatgatatta ctaaagatgt tgattattta     1620

aactcttatt attatttgga agcccaaaaa ttaagtaata atgttgaaaa tattactctt     1680

acaacttcag ttgaagaagc attaggttat agcaataaga tatacacatt tttacctagc     1740

ttagctgaaa aagtgaataa aggtgttcaa gcaggtttat tcttaaattg ggcgaatgaa     1800

gtagttgagg attttactac aaatattatg aaaaaagata cattggataa aatatcagat     1860

gtatcagcca taattccata tataggacct gccttaaata taggaaattc agcattaagg     1920

ggaaacttta agcaagcatt tgcaacagct ggtgtagctt ttttgttaga aggatttcca     1980

gagtttacaa tacctgcact cggtgtattt accttttata gttctattca agaaagagag     2040

aaaattatta aaactataga aaattgttta gaacaaagag ttaagagatg gaaagattca     2100

tatcaatgga tggtatcaaa ttggttgtca agaattacta ctcgatttaa tcatataagt     2160

tatcaaatgt atgattcttt gagttatcag gcagatgcaa tcaaagctaa aatagattta     2220

gaatataaaa aatactcagg aagtgataaa gaaaatataa aaagtcaagt tgaaaattta     2280

aaaaatagtt tagatgtaaa aatctcggaa gcaatgaata atataaataa atttatacga     2340

gaatgttctg taacatactt atttaaaaat atgctcccta aagtaattga tgaattaaat     2400

aagtttgatt taaaaactaa aacagaatta attaatctta tagatagtca taatattatt     2460

ctagttggtg aagtagatag attaaaagca aaagtaaatg agagttttga aaatacaata     2520

ccctttaata ttttttcata tactaataat tctttattaa aagatatgat taatgaatat     2580

ttcaatagta ttaatgattc aaaaattttg agcttacaaa ataaaaaaaa tactttgatg     2640

gatacatcag gatataacgc agaagtgaga gtagaaggca atgttcagct taatccaata     2700

tttccatttg actttaaatt aggtagttca ggggatgata gaggtaaagt tatagtaacc     2760

cagaatgaaa atattgtata taatgctatg tatgaaagtt ttagtattag tttttggatt     2820

aggataaata aatgggtaag taatttacct ggatatacta taattgatag tgttaaaaat     2880

aactcaggtt ggagtatagg tattattagt aattttttag tgtttacttt aaaacaaaat     2940

gaaaatagtg aacaagatat aaactttagt tatgatatat caaagaatgc tgcgggatat     3000

aataaatggt tttttgtaac tattactacc aatatgatgg gaaatatgat gatttatata     3060

aatggaaaat taatagatac tataaaagtt aaagagttaa ctggaattaa ttttagcaaa     3120

actataacat ttcaaatgaa taaaattcca aatactggct taattacctc agattctgat     3180

aacatcaata tgtggataag ggatttttat atctttgcta aagaattaga tgataaagat     3240

attaatatat tatttaatag cttgcaatat actaatgttg taaaagatta ttggggaaat     3300

gatttaagat atgataaaga atattacatg attaacgtaa attatatgaa tagatatatg     3360

tctaaaaaag gcaatggaat tgtttttaat acacgtaaaa ataataatga cttcaatgaa     3420

ggatataaaa ttataataaa aagaattaga ggaaatacaa atgatactag agtacgagga     3480

gaaaatgtat tatattttaa tactacaatt gataacaaac aatatagttt aggtatgtat     3540

aaaccttcta gaaatctagg gactgattta gttccactag gtgcattgga tcaaccaatg     3600

gatgagatac gtaaatatgg ttcgtttata atacaaccat gcaatacttt tgattactat     3660

gcatcacaat tatttttgtc aagtaatgca acaacaaata ggcttggaat actatcaatt     3720

ggtagttata gtttcaaact tggagatgac tattggttta atcacgaata tttaattcct     3780

gttataaaaa tagagcatta tgcttcatta ttagaatcaa catcaactca ttgggttttt     3840

gtacctgcaa gtgaataa                                                   3858


<210>  8
<211>  1285
<212>  PRT
<213>  Clostridium botulinum

<400>  8

Met Thr Trp Pro Val Lys Asp Phe Asn Tyr Ser Asp Pro Val Asn Asp 
1               5                   10                  15      


Asn Asp Ile Leu Tyr Leu Arg Ile Pro Gln Asn Lys Leu Ile Thr Thr 
            20                  25                  30          


Pro Val Lys Ala Phe Met Ile Thr Gln Asn Ile Trp Val Ile Pro Glu 
        35                  40                  45              


Arg Phe Ser Ser Asp Thr Asn Pro Ser Leu Ser Lys Pro Pro Arg Pro 
    50                  55                  60                  


Thr Ser Lys Tyr Gln Ser Tyr Tyr Asp Pro Ser Tyr Leu Ser Thr Asp 
65                  70                  75                  80  


Glu Gln Lys Asp Thr Phe Leu Lys Gly Ile Ile Lys Leu Phe Lys Arg 
                85                  90                  95      


Ile Asn Glu Arg Asp Ile Gly Lys Lys Leu Ile Asn Tyr Leu Val Val 
            100                 105                 110         


Gly Ser Pro Phe Met Gly Asp Ser Ser Thr Pro Glu Asp Thr Phe Asp 
        115                 120                 125             


Phe Thr Arg His Thr Thr Asn Ile Ala Val Glu Lys Phe Glu Asn Gly 
    130                 135                 140                 


Ser Trp Lys Val Thr Asn Ile Ile Thr Pro Ser Val Leu Ile Phe Gly 
145                 150                 155                 160 


Pro Leu Pro Asn Ile Leu Asp Tyr Thr Ala Ser Leu Thr Leu Gln Gly 
                165                 170                 175     


Gln Gln Ser Asn Pro Ser Phe Glu Gly Phe Gly Thr Leu Ser Ile Leu 
            180                 185                 190         


Lys Val Ala Pro Glu Phe Leu Leu Thr Phe Ser Asp Val Thr Ser Asn 
        195                 200                 205             


Gln Ser Ser Ala Val Leu Gly Lys Ser Ile Phe Cys Met Asp Pro Val 
    210                 215                 220                 


Ile Ala Leu Met His Glu Leu Thr His Ser Leu His Gln Leu Tyr Gly 
225                 230                 235                 240 


Ile Asn Ile Pro Ser Asp Lys Arg Ile Arg Pro Gln Val Ser Glu Gly 
                245                 250                 255     


Phe Phe Ser Gln Asp Gly Pro Asn Val Gln Phe Glu Glu Leu Tyr Thr 
            260                 265                 270         


Phe Gly Gly Ser Asp Val Glu Ile Ile Pro Gln Ile Glu Arg Leu Gln 
        275                 280                 285             


Leu Arg Glu Lys Ala Leu Gly His Tyr Lys Asp Ile Ala Lys Arg Leu 
    290                 295                 300                 


Asn Asn Ile Asn Lys Thr Ile Pro Ser Ser Trp Ser Ser Asn Ile Asp 
305                 310                 315                 320 


Lys Tyr Lys Lys Ile Phe Ser Glu Lys Tyr Asn Phe Asp Lys Asp Asn 
                325                 330                 335     


Thr Gly Asn Phe Val Val Asn Ile Asp Lys Phe Asn Ser Leu Tyr Ser 
            340                 345                 350         


Asp Leu Thr Asn Val Met Ser Glu Val Val Tyr Ser Ser Gln Tyr Asn 
        355                 360                 365             


Val Lys Asn Arg Thr His Tyr Phe Ser Lys His Tyr Leu Pro Val Phe 
    370                 375                 380                 


Ala Asn Ile Leu Asp Asp Asn Ile Tyr Thr Ile Ile Asn Gly Phe Asn 
385                 390                 395                 400 


Leu Thr Thr Lys Gly Phe Asn Ile Glu Asn Ser Gly Gln Asn Ile Glu 
                405                 410                 415     


Arg Asn Pro Ala Leu Gln Lys Leu Ser Ser Glu Ser Val Val Asp Leu 
            420                 425                 430         


Phe Thr Lys Val Cys Leu Arg Leu Thr Arg Asn Ser Arg Asp Asp Ser 
        435                 440                 445             


Thr Cys Ile Gln Val Lys Asn Asn Thr Leu Pro Tyr Val Ala Asp Lys 
    450                 455                 460                 


Asp Ser Ile Ser Gln Glu Ile Phe Glu Ser Gln Ile Ile Thr Asp Glu 
465                 470                 475                 480 


Thr Asn Val Glu Asn Tyr Ser Asp Asn Phe Ser Leu Asp Glu Ser Ile 
                485                 490                 495     


Leu Asp Ala Lys Val Pro Thr Asn Pro Glu Ala Val Asp Pro Leu Leu 
            500                 505                 510         


Pro Asn Val Asn Met Glu Pro Leu Asn Val Pro Gly Glu Glu Glu Val 
        515                 520                 525             


Phe Tyr Asp Asp Ile Thr Lys Asp Val Asp Tyr Leu Asn Ser Tyr Tyr 
    530                 535                 540                 


Tyr Leu Glu Ala Gln Lys Leu Ser Asn Asn Val Glu Asn Ile Thr Leu 
545                 550                 555                 560 


Thr Thr Ser Val Glu Glu Ala Leu Gly Tyr Ser Asn Lys Ile Tyr Thr 
                565                 570                 575     


Phe Leu Pro Ser Leu Ala Glu Lys Val Asn Lys Gly Val Gln Ala Gly 
            580                 585                 590         


Leu Phe Leu Asn Trp Ala Asn Glu Val Val Glu Asp Phe Thr Thr Asn 
        595                 600                 605             


Ile Met Lys Lys Asp Thr Leu Asp Lys Ile Ser Asp Val Ser Ala Ile 
    610                 615                 620                 


Ile Pro Tyr Ile Gly Pro Ala Leu Asn Ile Gly Asn Ser Ala Leu Arg 
625                 630                 635                 640 


Gly Asn Phe Lys Gln Ala Phe Ala Thr Ala Gly Val Ala Phe Leu Leu 
                645                 650                 655     


Glu Gly Phe Pro Glu Phe Thr Ile Pro Ala Leu Gly Val Phe Thr Phe 
            660                 665                 670         


Tyr Ser Ser Ile Gln Glu Arg Glu Lys Ile Ile Lys Thr Ile Glu Asn 
        675                 680                 685             


Cys Leu Glu Gln Arg Val Lys Arg Trp Lys Asp Ser Tyr Gln Trp Met 
    690                 695                 700                 


Val Ser Asn Trp Leu Ser Arg Ile Thr Thr Arg Phe Asn His Ile Ser 
705                 710                 715                 720 


Tyr Gln Met Tyr Asp Ser Leu Ser Tyr Gln Ala Asp Ala Ile Lys Ala 
                725                 730                 735     


Lys Ile Asp Leu Glu Tyr Lys Lys Tyr Ser Gly Ser Asp Lys Glu Asn 
            740                 745                 750         


Ile Lys Ser Gln Val Glu Asn Leu Lys Asn Ser Leu Asp Val Lys Ile 
        755                 760                 765             


Ser Glu Ala Met Asn Asn Ile Asn Lys Phe Ile Arg Glu Cys Ser Val 
    770                 775                 780                 


Thr Tyr Leu Phe Lys Asn Met Leu Pro Lys Val Ile Asp Glu Leu Asn 
785                 790                 795                 800 


Lys Phe Asp Leu Lys Thr Lys Thr Glu Leu Ile Asn Leu Ile Asp Ser 
                805                 810                 815     


His Asn Ile Ile Leu Val Gly Glu Val Asp Arg Leu Lys Ala Lys Val 
            820                 825                 830         


Asn Glu Ser Phe Glu Asn Thr Ile Pro Phe Asn Ile Phe Ser Tyr Thr 
        835                 840                 845             


Asn Asn Ser Leu Leu Lys Asp Met Ile Asn Glu Tyr Phe Asn Ser Ile 
    850                 855                 860                 


Asn Asp Ser Lys Ile Leu Ser Leu Gln Asn Lys Lys Asn Thr Leu Met 
865                 870                 875                 880 


Asp Thr Ser Gly Tyr Asn Ala Glu Val Arg Val Glu Gly Asn Val Gln 
                885                 890                 895     


Leu Asn Pro Ile Phe Pro Phe Asp Phe Lys Leu Gly Ser Ser Gly Asp 
            900                 905                 910         


Asp Arg Gly Lys Val Ile Val Thr Gln Asn Glu Asn Ile Val Tyr Asn 
        915                 920                 925             


Ala Met Tyr Glu Ser Phe Ser Ile Ser Phe Trp Ile Arg Ile Asn Lys 
    930                 935                 940                 


Trp Val Ser Asn Leu Pro Gly Tyr Thr Ile Ile Asp Ser Val Lys Asn 
945                 950                 955                 960 


Asn Ser Gly Trp Ser Ile Gly Ile Ile Ser Asn Phe Leu Val Phe Thr 
                965                 970                 975     


Leu Lys Gln Asn Glu Asn Ser Glu Gln Asp Ile Asn Phe Ser Tyr Asp 
            980                 985                 990         


Ile Ser Lys Asn Ala Ala Gly Tyr  Asn Lys Trp Phe Phe  Val Thr Ile 
        995                 1000                 1005             


Thr Thr  Asn Met Met Gly Asn  Met Met Ile Tyr Ile  Asn Gly Lys 
    1010                 1015                 1020             


Leu Ile  Asp Thr Ile Lys Val  Lys Glu Leu Thr Gly  Ile Asn Phe 
    1025                 1030                 1035             


Ser Lys  Thr Ile Thr Phe Gln  Met Asn Lys Ile Pro  Asn Thr Gly 
    1040                 1045                 1050             


Leu Ile  Thr Ser Asp Ser Asp  Asn Ile Asn Met Trp  Ile Arg Asp 
    1055                 1060                 1065             


Phe Tyr  Ile Phe Ala Lys Glu  Leu Asp Asp Lys Asp  Ile Asn Ile 
    1070                 1075                 1080             


Leu Phe  Asn Ser Leu Gln Tyr  Thr Asn Val Val Lys  Asp Tyr Trp 
    1085                 1090                 1095             


Gly Asn  Asp Leu Arg Tyr Asp  Lys Glu Tyr Tyr Met  Ile Asn Val 
    1100                 1105                 1110             


Asn Tyr  Met Asn Arg Tyr Met  Ser Lys Lys Gly Asn  Gly Ile Val 
    1115                 1120                 1125             


Phe Asn  Thr Arg Lys Asn Asn  Asn Asp Phe Asn Glu  Gly Tyr Lys 
    1130                 1135                 1140             


Ile Ile  Ile Lys Arg Ile Arg  Gly Asn Thr Asn Asp  Thr Arg Val 
    1145                 1150                 1155             


Arg Gly  Glu Asn Val Leu Tyr  Phe Asn Thr Thr Ile  Asp Asn Lys 
    1160                 1165                 1170             


Gln Tyr  Ser Leu Gly Met Tyr  Lys Pro Ser Arg Asn  Leu Gly Thr 
    1175                 1180                 1185             


Asp Leu  Val Pro Leu Gly Ala  Leu Asp Gln Pro Met  Asp Glu Ile 
    1190                 1195                 1200             


Arg Lys  Tyr Gly Ser Phe Ile  Ile Gln Pro Cys Asn  Thr Phe Asp 
    1205                 1210                 1215             


Tyr Tyr  Ala Ser Gln Leu Phe  Leu Ser Ser Asn Ala  Thr Thr Asn 
    1220                 1225                 1230             


Arg Leu  Gly Ile Leu Ser Ile  Gly Ser Tyr Ser Phe  Lys Leu Gly 
    1235                 1240                 1245             


Asp Asp  Tyr Trp Phe Asn His  Glu Tyr Leu Ile Pro  Val Ile Lys 
    1250                 1255                 1260             


Ile Glu  His Tyr Ala Ser Leu  Leu Glu Ser Thr Ser  Thr His Trp 
    1265                 1270                 1275             


Val Phe  Val Pro Ala Ser Glu  
    1280                 1285 


<210>  9
<211>  3756
<212>  DNA
<213>  Clostridium botulinum

<400>  9
atgccaaaaa ttaatagttt taattataat gatcctgtta atgatagaac aattttatat       60

attaaaccag gcggttgtca agaattttat aaatcattta atattatgaa aaatatttgg      120

ataattccag agagaaatgt aattggtaca accccccaag attttcatcc gcctacttca      180

ttaaaaaatg gagatagtag ttattatgac cctaattatt tacaaagtga tgaagaaaag      240

gatagatttt taaaaatagt cacaaaaata tttaatagaa taaataataa tctttcagga      300

gggattttat tagaagaact gtcaaaagct aatccatatt tagggaatga taatactcca      360

gataatcaat tccatattgg tgatgcatca gcagttgaga ttaaattctc aaatggtagc      420

caagacatac tattacctaa tgttattata atgggagcag agcctgattt atttgaaact      480

aacagttcca atatttctct aagaaataat tatatgccaa gcaatcaccg ttttggatca      540

atagctatag taacattctc acctgaatat tcttttagat ttaatgataa ttgtatgaat      600

gaatttattc aagatcctgc tcttacatta atgcatgaat taatacattc attacatgga      660

ctatatgggg ctaaagggat tactacaaag tatactataa cacaaaaaca aaatccccta      720

ataacaaata taagaggtac aaatattgaa gaattcttaa cttttggagg tactgattta      780

aacattatta ctagtgctca gtccaatgat atctatacta atcttctagc tgattataaa      840

aaaatagcgt ctaaacttag caaagtacaa gtatctaatc cactacttaa tccttataaa      900

gatgtttttg aagcaaagta tggattagat aaagatgcta gcggaattta ttcggtaaat      960

ataaacaaat ttaatgatat ttttaaaaaa ttatacagct ttacggaatt tgatttacga     1020

actaaatttc aagttaaatg taggcaaact tatattggac agtataaata cttcaaactt     1080

tcaaacttgt taaatgattc tatttataat atatcagaag gctataatat aaataattta     1140

aaggtaaatt ttagaggaca gaatgcaaat ttaaatccta gaattattac accaattaca     1200

ggtagaggac tagtaaaaaa aatcattaga ttttgtaaaa atattgtttc tgtaaaaggc     1260

ataaggaaat caatatgtat cgaaataaat aatggtgagt tattttttgt ggcttccgag     1320

aatagttata atgatgataa tataaatact cctaaagaaa ttgacgatac agtaacttca     1380

aataataatt atgaaaatga tttagatcag gttattttaa attttaatag tgaatcagca     1440

cctggacttt cagatgaaaa attaaattta actatccaaa atgatgctta tataccaaaa     1500

tatgattcta atggaacaag tgatatagaa caacatgatg ttaatgaact taatgtattt     1560

ttctatttag atgcacagaa agtgcccgaa ggtgaaaata atgtcaatct cacctcttca     1620

attgatacag cattattaga acaacctaaa atatatacat ttttttcatc agaatttatt     1680

aataatgtca ataaacctgt gcaagcagca ttatttgtaa gctggataca acaagtgtta     1740

gtagatttta ctactgaagc taaccaaaaa agtactgttg ataaaattgc agatatttct     1800

atagttgttc catatatagg tcttgcttta aatataggaa atgaagcaca aaaaggaaat     1860

tttaaagatg cacttgaatt attaggagca ggtattttat tagaatttga acccgagctt     1920

ttaattccta caattttagt attcacgata aaatcttttt taggttcatc tgataataaa     1980

aataaagtta ttaaagcaat aaataatgca ttgaaagaaa gagatgaaaa atggaaagaa     2040

gtatatagtt ttatagtatc gaattggatg actaaaatta atacacaatt taataaaaga     2100

aaagaacaaa tgtatcaagc tttacaaaat caagtaaatg caattaaaac aataatagaa     2160

tctaagtata atagttatac tttagaggaa aaaaatgagc ttacaaataa atatgatatt     2220

aagcaaatag aaaatgaact taatcaaaag gtttctatag caatgaataa tatagacagg     2280

ttcttaactg aaagttctat atcctattta atgaaaataa taaatgaagt aaaaattaat     2340

aaattaagag aatatgatga gaatgtcaaa acgtatttat tgaattatat tatacaacat     2400

ggatcaatct tgggagagag tcagcaagaa ctaaattcta tggtaactga taccctaaat     2460

aatagtattc cttttaagct ttcttcttat acagatgata aaattttaat ttcatatttt     2520

aataaattct ttaagagaat taaaagtagt tcagttttaa atatgagata taaaaatgat     2580

aaatacgtag atacttcagg atatgattca aatataaata ttaatggaga tgtatataaa     2640

tatccaacta ataaaaatca atttggaata tataatgata aacttagtga agttaatata     2700

tctcaaaatg attacattat atatgataat aaatataaaa attttagtat tagtttttgg     2760

gtaagaattc ctaactatga taataagata gtaaatgtta ataatgaata cactataata     2820

aattgtatga gagataataa ttcaggatgg aaagtatctc ttaatcataa tgaaataatt     2880

tggacattcg aagataatcg aggaattaat caaaaattag catttaacta tggtaacgca     2940

aatggtattt ctgattatat aaataagtgg atttttgtaa ctataactaa tgatagatta     3000

ggagattcta aactttatat taatggaaat ttaatagatc aaaaatcaat tttaaattta     3060

ggtaatattc atgttagtga caatatatta tttaaaatag ttaattgtag ttatacaaga     3120

tatattggta ttagatattt taatattttt gataaagaat tagatgaaac agaaattcaa     3180

actttatata gcaatgaacc taatacaaat attttgaagg atttttgggg aaattatttg     3240

ctttatgaca aagaatacta tttattaaat gtgttaaaac caaataactt tattgatagg     3300

agaaaagatt ctactttaag cattaataat ataagaagca ctattctttt agctaataga     3360

ttatatagtg gaataaaagt taaaatacaa agagttaata atagtagtac taacgataat     3420

cttgttagaa agaatgatca ggtatatatt aattttgtag ccagcaaaac tcacttattt     3480

ccattatatg ctgatacagc taccacaaat aaagagaaaa caataaaaat atcatcatct     3540

ggcaatagat ttaatcaagt agtagttatg aattcagtag gaaattgtac aatgaatttt     3600

aaaaataata atggaaataa tattgggttg ttaggtttca aggcagatac tgtcgttgct     3660

agtacttggt attatacaca tatgagagat catacaaaca gcaatggatg tttttggaac     3720

tttatttctg aagaacatgg atggcaagaa aaataa                               3756


<210>  10
<211>  1251
<212>  PRT
<213>  Clostridium botulinum

<400>  10

Met Pro Lys Ile Asn Ser Phe Asn Tyr Asn Asp Pro Val Asn Asp Arg 
1               5                   10                  15      


Thr Ile Leu Tyr Ile Lys Pro Gly Gly Cys Gln Glu Phe Tyr Lys Ser 
            20                  25                  30          


Phe Asn Ile Met Lys Asn Ile Trp Ile Ile Pro Glu Arg Asn Val Ile 
        35                  40                  45              


Gly Thr Thr Pro Gln Asp Phe His Pro Pro Thr Ser Leu Lys Asn Gly 
    50                  55                  60                  


Asp Ser Ser Tyr Tyr Asp Pro Asn Tyr Leu Gln Ser Asp Glu Glu Lys 
65                  70                  75                  80  


Asp Arg Phe Leu Lys Ile Val Thr Lys Ile Phe Asn Arg Ile Asn Asn 
                85                  90                  95      


Asn Leu Ser Gly Gly Ile Leu Leu Glu Glu Leu Ser Lys Ala Asn Pro 
            100                 105                 110         


Tyr Leu Gly Asn Asp Asn Thr Pro Asp Asn Gln Phe His Ile Gly Asp 
        115                 120                 125             


Ala Ser Ala Val Glu Ile Lys Phe Ser Asn Gly Ser Gln Asp Ile Leu 
    130                 135                 140                 


Leu Pro Asn Val Ile Ile Met Gly Ala Glu Pro Asp Leu Phe Glu Thr 
145                 150                 155                 160 


Asn Ser Ser Asn Ile Ser Leu Arg Asn Asn Tyr Met Pro Ser Asn His 
                165                 170                 175     


Arg Phe Gly Ser Ile Ala Ile Val Thr Phe Ser Pro Glu Tyr Ser Phe 
            180                 185                 190         


Arg Phe Asn Asp Asn Cys Met Asn Glu Phe Ile Gln Asp Pro Ala Leu 
        195                 200                 205             


Thr Leu Met His Glu Leu Ile His Ser Leu His Gly Leu Tyr Gly Ala 
    210                 215                 220                 


Lys Gly Ile Thr Thr Lys Tyr Thr Ile Thr Gln Lys Gln Asn Pro Leu 
225                 230                 235                 240 


Ile Thr Asn Ile Arg Gly Thr Asn Ile Glu Glu Phe Leu Thr Phe Gly 
                245                 250                 255     


Gly Thr Asp Leu Asn Ile Ile Thr Ser Ala Gln Ser Asn Asp Ile Tyr 
            260                 265                 270         


Thr Asn Leu Leu Ala Asp Tyr Lys Lys Ile Ala Ser Lys Leu Ser Lys 
        275                 280                 285             


Val Gln Val Ser Asn Pro Leu Leu Asn Pro Tyr Lys Asp Val Phe Glu 
    290                 295                 300                 


Ala Lys Tyr Gly Leu Asp Lys Asp Ala Ser Gly Ile Tyr Ser Val Asn 
305                 310                 315                 320 


Ile Asn Lys Phe Asn Asp Ile Phe Lys Lys Leu Tyr Ser Phe Thr Glu 
                325                 330                 335     


Phe Asp Leu Arg Thr Lys Phe Gln Val Lys Cys Arg Gln Thr Tyr Ile 
            340                 345                 350         


Gly Gln Tyr Lys Tyr Phe Lys Leu Ser Asn Leu Leu Asn Asp Ser Ile 
        355                 360                 365             


Tyr Asn Ile Ser Glu Gly Tyr Asn Ile Asn Asn Leu Lys Val Asn Phe 
    370                 375                 380                 


Arg Gly Gln Asn Ala Asn Leu Asn Pro Arg Ile Ile Thr Pro Ile Thr 
385                 390                 395                 400 


Gly Arg Gly Leu Val Lys Lys Ile Ile Arg Phe Cys Lys Asn Ile Val 
                405                 410                 415     


Ser Val Lys Gly Ile Arg Lys Ser Ile Cys Ile Glu Ile Asn Asn Gly 
            420                 425                 430         


Glu Leu Phe Phe Val Ala Ser Glu Asn Ser Tyr Asn Asp Asp Asn Ile 
        435                 440                 445             


Asn Thr Pro Lys Glu Ile Asp Asp Thr Val Thr Ser Asn Asn Asn Tyr 
    450                 455                 460                 


Glu Asn Asp Leu Asp Gln Val Ile Leu Asn Phe Asn Ser Glu Ser Ala 
465                 470                 475                 480 


Pro Gly Leu Ser Asp Glu Lys Leu Asn Leu Thr Ile Gln Asn Asp Ala 
                485                 490                 495     


Tyr Ile Pro Lys Tyr Asp Ser Asn Gly Thr Ser Asp Ile Glu Gln His 
            500                 505                 510         


Asp Val Asn Glu Leu Asn Val Phe Phe Tyr Leu Asp Ala Gln Lys Val 
        515                 520                 525             


Pro Glu Gly Glu Asn Asn Val Asn Leu Thr Ser Ser Ile Asp Thr Ala 
    530                 535                 540                 


Leu Leu Glu Gln Pro Lys Ile Tyr Thr Phe Phe Ser Ser Glu Phe Ile 
545                 550                 555                 560 


Asn Asn Val Asn Lys Pro Val Gln Ala Ala Leu Phe Val Ser Trp Ile 
                565                 570                 575     


Gln Gln Val Leu Val Asp Phe Thr Thr Glu Ala Asn Gln Lys Ser Thr 
            580                 585                 590         


Val Asp Lys Ile Ala Asp Ile Ser Ile Val Val Pro Tyr Ile Gly Leu 
        595                 600                 605             


Ala Leu Asn Ile Gly Asn Glu Ala Gln Lys Gly Asn Phe Lys Asp Ala 
    610                 615                 620                 


Leu Glu Leu Leu Gly Ala Gly Ile Leu Leu Glu Phe Glu Pro Glu Leu 
625                 630                 635                 640 


Leu Ile Pro Thr Ile Leu Val Phe Thr Ile Lys Ser Phe Leu Gly Ser 
                645                 650                 655     


Ser Asp Asn Lys Asn Lys Val Ile Lys Ala Ile Asn Asn Ala Leu Lys 
            660                 665                 670         


Glu Arg Asp Glu Lys Trp Lys Glu Val Tyr Ser Phe Ile Val Ser Asn 
        675                 680                 685             


Trp Met Thr Lys Ile Asn Thr Gln Phe Asn Lys Arg Lys Glu Gln Met 
    690                 695                 700                 


Tyr Gln Ala Leu Gln Asn Gln Val Asn Ala Ile Lys Thr Ile Ile Glu 
705                 710                 715                 720 


Ser Lys Tyr Asn Ser Tyr Thr Leu Glu Glu Lys Asn Glu Leu Thr Asn 
                725                 730                 735     


Lys Tyr Asp Ile Lys Gln Ile Glu Asn Glu Leu Asn Gln Lys Val Ser 
            740                 745                 750         


Ile Ala Met Asn Asn Ile Asp Arg Phe Leu Thr Glu Ser Ser Ile Ser 
        755                 760                 765             


Tyr Leu Met Lys Ile Ile Asn Glu Val Lys Ile Asn Lys Leu Arg Glu 
    770                 775                 780                 


Tyr Asp Glu Asn Val Lys Thr Tyr Leu Leu Asn Tyr Ile Ile Gln His 
785                 790                 795                 800 


Gly Ser Ile Leu Gly Glu Ser Gln Gln Glu Leu Asn Ser Met Val Thr 
                805                 810                 815     


Asp Thr Leu Asn Asn Ser Ile Pro Phe Lys Leu Ser Ser Tyr Thr Asp 
            820                 825                 830         


Asp Lys Ile Leu Ile Ser Tyr Phe Asn Lys Phe Phe Lys Arg Ile Lys 
        835                 840                 845             


Ser Ser Ser Val Leu Asn Met Arg Tyr Lys Asn Asp Lys Tyr Val Asp 
    850                 855                 860                 


Thr Ser Gly Tyr Asp Ser Asn Ile Asn Ile Asn Gly Asp Val Tyr Lys 
865                 870                 875                 880 


Tyr Pro Thr Asn Lys Asn Gln Phe Gly Ile Tyr Asn Asp Lys Leu Ser 
                885                 890                 895     


Glu Val Asn Ile Ser Gln Asn Asp Tyr Ile Ile Tyr Asp Asn Lys Tyr 
            900                 905                 910         


Lys Asn Phe Ser Ile Ser Phe Trp Val Arg Ile Pro Asn Tyr Asp Asn 
        915                 920                 925             


Lys Ile Val Asn Val Asn Asn Glu Tyr Thr Ile Ile Asn Cys Met Arg 
    930                 935                 940                 


Asp Asn Asn Ser Gly Trp Lys Val Ser Leu Asn His Asn Glu Ile Ile 
945                 950                 955                 960 


Trp Thr Phe Glu Asp Asn Arg Gly Ile Asn Gln Lys Leu Ala Phe Asn 
                965                 970                 975     


Tyr Gly Asn Ala Asn Gly Ile Ser Asp Tyr Ile Asn Lys Trp Ile Phe 
            980                 985                 990         


Val Thr Ile Thr Asn Asp Arg Leu  Gly Asp Ser Lys Leu  Tyr Ile Asn 
        995                 1000                 1005             


Gly Asn  Leu Ile Asp Gln Lys  Ser Ile Leu Asn Leu  Gly Asn Ile 
    1010                 1015                 1020             


His Val  Ser Asp Asn Ile Leu  Phe Lys Ile Val Asn  Cys Ser Tyr 
    1025                 1030                 1035             


Thr Arg  Tyr Ile Gly Ile Arg  Tyr Phe Asn Ile Phe  Asp Lys Glu 
    1040                 1045                 1050             


Leu Asp  Glu Thr Glu Ile Gln  Thr Leu Tyr Ser Asn  Glu Pro Asn 
    1055                 1060                 1065             


Thr Asn  Ile Leu Lys Asp Phe  Trp Gly Asn Tyr Leu  Leu Tyr Asp 
    1070                 1075                 1080             


Lys Glu  Tyr Tyr Leu Leu Asn  Val Leu Lys Pro Asn  Asn Phe Ile 
    1085                 1090                 1095             


Asp Arg  Arg Lys Asp Ser Thr  Leu Ser Ile Asn Asn  Ile Arg Ser 
    1100                 1105                 1110             


Thr Ile  Leu Leu Ala Asn Arg  Leu Tyr Ser Gly Ile  Lys Val Lys 
    1115                 1120                 1125             


Ile Gln  Arg Val Asn Asn Ser  Ser Thr Asn Asp Asn  Leu Val Arg 
    1130                 1135                 1140             


Lys Asn  Asp Gln Val Tyr Ile  Asn Phe Val Ala Ser  Lys Thr His 
    1145                 1150                 1155             


Leu Phe  Pro Leu Tyr Ala Asp  Thr Ala Thr Thr Asn  Lys Glu Lys 
    1160                 1165                 1170             


Thr Ile  Lys Ile Ser Ser Ser  Gly Asn Arg Phe Asn  Gln Val Val 
    1175                 1180                 1185             


Val Met  Asn Ser Val Gly Asn  Cys Thr Met Asn Phe  Lys Asn Asn 
    1190                 1195                 1200             


Asn Gly  Asn Asn Ile Gly Leu  Leu Gly Phe Lys Ala  Asp Thr Val 
    1205                 1210                 1215             


Val Ala  Ser Thr Trp Tyr Tyr  Thr His Met Arg Asp  His Thr Asn 
    1220                 1225                 1230             


Ser Asn  Gly Cys Phe Trp Asn  Phe Ile Ser Glu Glu  His Gly Trp 
    1235                 1240                 1245             


Gln Glu  Lys 
    1250     


<210>  11
<211>  3843
<212>  DNA
<213>  Clostridium botulinum

<400>  11
atgccagttg taataaatag ttttaattat aatgaccctg ttaatgatga gacaatttta       60

tacatgcaga aaccatatga agaaagaagt agaaaatatt ataaagcttt tgagattatg      120

cctaatgttt ggataatgcc tgagagagat acaataggaa ctaagcctga tgagtttcag      180

gtgccggatt cattaaagaa cggaagtagt gcttattatg atcctaatta tttaaccact      240

gatgctgaaa aagatagata tttaaaaaca atgataaaat tatttaatag aattaatagt      300

aatcctacag ggaaagtttt gttagaagaa gtatcaaatg ctagaccata tttaggagat      360

gatgacacgc taattaatga attccttcca gttaatgtaa ctacaagtgt taatataaaa      420

ttttcaactg atgttgaaag ttcaataata tcgaatcttc ttgtattggg agcaggacct      480

gatatattta aagcttactg tacccccctt gtaaggttta ataagtcaga taaattaatt      540

gaaccaagta atcatggttt tggatcaatt aatatcttga cattttcacc tgagtatgaa      600

catattttta atgatattag tggagggaat cataatagta cagaatcatt tattgcagat      660

cctgcaattt cactagctca tgaattgata catgcactac atggattata cggggctaag      720

gcagttactc ataaagagtc tctagtagca gagcgaggac ctcttatgat agccgaaaag      780

cccataaggc tagaagaatt tttaactttt ggaggtgagg atttaaatat cattcctagt      840

gctatgaagg aaaaaatata taacgatctt ttagctaact atgaaaaaat agctactaga      900

cttagagaag ttaatacggc tcctcctgga tatgatatta atgaatataa agattatttt      960

caatggaagt atggactaga tagaaatgca gatggaagtt atactgtgaa tagaaataaa     1020

tttaatgaaa tttataaaaa attatatagc tttacagaga ttgacttagc aaataaattt     1080

aaagtaaaat gtagaaatac ttattttatt aaatatggat ttgtaaaagt tccaaatttg     1140

ttagatgatg atatttatac tgtatcagag gggtttaata taggtaattt agcagtaaac     1200

aatcgcggac aaaatataaa tttaaatcct aaaattattg attccattcc agataaaggt     1260

ttagtggaaa agattattaa attttgtaag agcattattc ctagaaaagg tacgaagcag     1320

tcaccgtcac tatgcattag agtaaataat agggagttat tttttgtagc ttcagaaagt     1380

agctataatg aaagtgatat taatacacct aaagaaattg acgatacaac aaatctaaat     1440

aataattata gaaataattt agatgaagtt attttagatt ataatagtga gacaatacct     1500

caaatatcaa atcgaacatt aaatacactt gtacaagaca atagttatgt gccaagatat     1560

gattctaatg gaacaagtga aatagaggaa tatgatgttg ttgactttaa tgtatttttc     1620

tatttacatg cacaaaaagt accagaaggt gaaaccaata taagtttaac ttcttcaatt     1680

gatacagcat tattagaaga atccaaagta tatacatttt tttcttcaga gtttatcgat     1740

actatcaata aacctgtaaa tgcagcacta tttatagatt ggataagcaa agtaataaga     1800

gattttacca ctgaagctac acaaaaaagt actgttgata agattgcaga catatcttta     1860

attgtaccct atgtaggtct tgctttgaat atagttattg aggcagaaaa aggaaatttt     1920

gaggaggcat ttgaattatt aggagcgggt attttattag aatttgtgcc agagcttaca     1980

attcctgtaa ttttagtgtt tacgataaaa tcctatatag attcatatga gaataaaaat     2040

aaagcaatta aagcaataaa taattcatta atcgaaagag aagcaaagtg gaaagaaata     2100

tatagttgga tagtatcaaa ttggcttact agaattaata cgcaatttaa taaaagaaaa     2160

gagcaaatgt atcaggcttt acaaaatcaa gtagatgcaa taaaaacagc aatagaatat     2220

aaatataata attatacttc agatgagaaa aatagacttg aatctaaata taatatcaat     2280

aatatagaag aagaattgaa taaaaaagtt tctttagcaa tgaaaaatat agaaagattt     2340

atgacagaaa gttctatatc ttatttaatg aaattaataa atgaagccga agttggtaaa     2400

ttaaaagaat atgataaaca tgttaagagc gatttattag actatattct ctaccataaa     2460

ttaatcttag gagagcagac aaaggaatta attgatttgg tgactagtac tttgaatagt     2520

agtattccat ttgaactttc ttcatatact aatgataaaa ttctaattat atattttaat     2580

agattatata aaaaaattaa agatagttct attttagata tgcgatatga aaataataaa     2640

tttatagata tctctggata tggttcaaat ataagcatta atggaaacgt atatatttat     2700

tcaacaaata gaaatcaatt tggaatatat agtggtaggc ttagtgaagt taatatagct     2760

caaaataatg atattatata caatagtaga tatcaaaatt ttagtattag tttctgggta     2820

accattccta aacactacag acctatgaat cgtaatcggg aatacactat aataaattgt     2880

atggggaata ataattcggg atggaaaata tcacttagaa ctattagaga ttgtgaaata     2940

atttggactt tacaagatac ttccggaaat aaggaaaaat taatttttag gtatgaagaa     3000

cttgctagta tatctgatta tataaataaa tggatttttg taactattac taataataga     3060

ttaggcaatt ctagaattta catcaatgga aatttaatag ttgaaaaatc aatttcgaat     3120

ttaggtgata ttcatgttag tgataatata ttatttaaaa ttgttggttg tgatgatgaa     3180

acgtatgttg gtataagata ttttaaagtt tttaatacgg aattagataa aacagaaatt     3240

gagactttat atagtaatga gccagatcca agtatcttaa aagactattg gggaaattat     3300

ttgctatata ataaaaaata ttatttattc aatttactaa gaaaagataa gtatattact     3360

cggaattcag gcattttaaa tattaatcaa caaagaggtg ttactggagg catatctgtt     3420

tttttgaact ataaattata tgaaggagta gaagttatta taagaaaaaa tgctcctata     3480

gatatatcta atacagataa ttttgttaga aaaaacgatc tagcatacat taatgtagta     3540

gatcatggtg tagaatatcg gttatatgct gatatatcaa ttacaaaatc agagaaaata     3600

ataaaattaa taagaacatc taatccaaac gatagcttag gtcaaattat agttatggat     3660

tcaataggaa ataattgcac aatgaatttt caaaacaatg atgggagcaa tataggatta     3720

ctaggttttc attcagatga tttggttgct agtagttggt attataacca tatacgaaga     3780

aacactagca gtaatggatg cttttggagt tttatttcta aagagcatgg ttggaaagaa     3840

taa                                                                   3843


<210>  12
<211>  1280
<212>  PRT
<213>  Clostridium botulinum

<400>  12

Met Pro Val Val Ile Asn Ser Phe Asn Tyr Asn Asp Pro Val Asn Asp 
1               5                   10                  15      


Glu Thr Ile Leu Tyr Met Gln Lys Pro Tyr Glu Glu Arg Ser Arg Lys 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Glu Ile Met Pro Asn Val Trp Ile Met Pro Glu 
        35                  40                  45              


Arg Asp Thr Ile Gly Thr Lys Pro Asp Glu Phe Gln Val Pro Asp Ser 
    50                  55                  60                  


Leu Lys Asn Gly Ser Ser Ala Tyr Tyr Asp Pro Asn Tyr Leu Thr Thr 
65                  70                  75                  80  


Asp Ala Glu Lys Asp Arg Tyr Leu Lys Thr Met Ile Lys Leu Phe Asn 
                85                  90                  95      


Arg Ile Asn Ser Asn Pro Thr Gly Lys Val Leu Leu Glu Glu Val Ser 
            100                 105                 110         


Asn Ala Arg Pro Tyr Leu Gly Asp Asp Asp Thr Leu Ile Asn Glu Phe 
        115                 120                 125             


Leu Pro Val Asn Val Thr Thr Ser Val Asn Ile Lys Phe Ser Thr Asp 
    130                 135                 140                 


Val Glu Ser Ser Ile Ile Ser Asn Leu Leu Val Leu Gly Ala Gly Pro 
145                 150                 155                 160 


Asp Ile Phe Lys Ala Tyr Cys Thr Pro Leu Val Arg Phe Asn Lys Ser 
                165                 170                 175     


Asp Lys Leu Ile Glu Pro Ser Asn His Gly Phe Gly Ser Ile Asn Ile 
            180                 185                 190         


Leu Thr Phe Ser Pro Glu Tyr Glu His Ile Phe Asn Asp Ile Ser Gly 
        195                 200                 205             


Gly Asn His Asn Ser Thr Glu Ser Phe Ile Ala Asp Pro Ala Ile Ser 
    210                 215                 220                 


Leu Ala His Glu Leu Ile His Ala Leu His Gly Leu Tyr Gly Ala Lys 
225                 230                 235                 240 


Ala Val Thr His Lys Glu Ser Leu Val Ala Glu Arg Gly Pro Leu Met 
                245                 250                 255     


Ile Ala Glu Lys Pro Ile Arg Leu Glu Glu Phe Leu Thr Phe Gly Gly 
            260                 265                 270         


Glu Asp Leu Asn Ile Ile Pro Ser Ala Met Lys Glu Lys Ile Tyr Asn 
        275                 280                 285             


Asp Leu Leu Ala Asn Tyr Glu Lys Ile Ala Thr Arg Leu Arg Glu Val 
    290                 295                 300                 


Asn Thr Ala Pro Pro Gly Tyr Asp Ile Asn Glu Tyr Lys Asp Tyr Phe 
305                 310                 315                 320 


Gln Trp Lys Tyr Gly Leu Asp Arg Asn Ala Asp Gly Ser Tyr Thr Val 
                325                 330                 335     


Asn Arg Asn Lys Phe Asn Glu Ile Tyr Lys Lys Leu Tyr Ser Phe Thr 
            340                 345                 350         


Glu Ile Asp Leu Ala Asn Lys Phe Lys Val Lys Cys Arg Asn Thr Tyr 
        355                 360                 365             


Phe Ile Lys Tyr Gly Phe Val Lys Val Pro Asn Leu Leu Asp Asp Asp 
    370                 375                 380                 


Ile Tyr Thr Val Ser Glu Gly Phe Asn Ile Gly Asn Leu Ala Val Asn 
385                 390                 395                 400 


Asn Arg Gly Gln Asn Ile Asn Leu Asn Pro Lys Ile Ile Asp Ser Ile 
                405                 410                 415     


Pro Asp Lys Gly Leu Val Glu Lys Ile Ile Lys Phe Cys Lys Ser Ile 
            420                 425                 430         


Ile Pro Arg Lys Gly Thr Lys Gln Ser Pro Ser Leu Cys Ile Arg Val 
        435                 440                 445             


Asn Asn Arg Glu Leu Phe Phe Val Ala Ser Glu Ser Ser Tyr Asn Glu 
    450                 455                 460                 


Ser Asp Ile Asn Thr Pro Lys Glu Ile Asp Asp Thr Thr Asn Leu Asn 
465                 470                 475                 480 


Asn Asn Tyr Arg Asn Asn Leu Asp Glu Val Ile Leu Asp Tyr Asn Ser 
                485                 490                 495     


Glu Thr Ile Pro Gln Ile Ser Asn Arg Thr Leu Asn Thr Leu Val Gln 
            500                 505                 510         


Asp Asn Ser Tyr Val Pro Arg Tyr Asp Ser Asn Gly Thr Ser Glu Ile 
        515                 520                 525             


Glu Glu Tyr Asp Val Val Asp Phe Asn Val Phe Phe Tyr Leu His Ala 
    530                 535                 540                 


Gln Lys Val Pro Glu Gly Glu Thr Asn Ile Ser Leu Thr Ser Ser Ile 
545                 550                 555                 560 


Asp Thr Ala Leu Leu Glu Glu Ser Lys Val Tyr Thr Phe Phe Ser Ser 
                565                 570                 575     


Glu Phe Ile Asp Thr Ile Asn Lys Pro Val Asn Ala Ala Leu Phe Ile 
            580                 585                 590         


Asp Trp Ile Ser Lys Val Ile Arg Asp Phe Thr Thr Glu Ala Thr Gln 
        595                 600                 605             


Lys Ser Thr Val Asp Lys Ile Ala Asp Ile Ser Leu Ile Val Pro Tyr 
    610                 615                 620                 


Val Gly Leu Ala Leu Asn Ile Val Ile Glu Ala Glu Lys Gly Asn Phe 
625                 630                 635                 640 


Glu Glu Ala Phe Glu Leu Leu Gly Ala Gly Ile Leu Leu Glu Phe Val 
                645                 650                 655     


Pro Glu Leu Thr Ile Pro Val Ile Leu Val Phe Thr Ile Lys Ser Tyr 
            660                 665                 670         


Ile Asp Ser Tyr Glu Asn Lys Asn Lys Ala Ile Lys Ala Ile Asn Asn 
        675                 680                 685             


Ser Leu Ile Glu Arg Glu Ala Lys Trp Lys Glu Ile Tyr Ser Trp Ile 
    690                 695                 700                 


Val Ser Asn Trp Leu Thr Arg Ile Asn Thr Gln Phe Asn Lys Arg Lys 
705                 710                 715                 720 


Glu Gln Met Tyr Gln Ala Leu Gln Asn Gln Val Asp Ala Ile Lys Thr 
                725                 730                 735     


Ala Ile Glu Tyr Lys Tyr Asn Asn Tyr Thr Ser Asp Glu Lys Asn Arg 
            740                 745                 750         


Leu Glu Ser Lys Tyr Asn Ile Asn Asn Ile Glu Glu Glu Leu Asn Lys 
        755                 760                 765             


Lys Val Ser Leu Ala Met Lys Asn Ile Glu Arg Phe Met Thr Glu Ser 
    770                 775                 780                 


Ser Ile Ser Tyr Leu Met Lys Leu Ile Asn Glu Ala Glu Val Gly Lys 
785                 790                 795                 800 


Leu Lys Glu Tyr Asp Lys His Val Lys Ser Asp Leu Leu Asp Tyr Ile 
                805                 810                 815     


Leu Tyr His Lys Leu Ile Leu Gly Glu Gln Thr Lys Glu Leu Ile Asp 
            820                 825                 830         


Leu Val Thr Ser Thr Leu Asn Ser Ser Ile Pro Phe Glu Leu Ser Ser 
        835                 840                 845             


Tyr Thr Asn Asp Lys Ile Leu Ile Ile Tyr Phe Asn Arg Leu Tyr Lys 
    850                 855                 860                 


Lys Ile Lys Asp Ser Ser Ile Leu Asp Met Arg Tyr Glu Asn Asn Lys 
865                 870                 875                 880 


Phe Ile Asp Ile Ser Gly Tyr Gly Ser Asn Ile Ser Ile Asn Gly Asn 
                885                 890                 895     


Val Tyr Ile Tyr Ser Thr Asn Arg Asn Gln Phe Gly Ile Tyr Ser Gly 
            900                 905                 910         


Arg Leu Ser Glu Val Asn Ile Ala Gln Asn Asn Asp Ile Ile Tyr Asn 
        915                 920                 925             


Ser Arg Tyr Gln Asn Phe Ser Ile Ser Phe Trp Val Thr Ile Pro Lys 
    930                 935                 940                 


His Tyr Arg Pro Met Asn Arg Asn Arg Glu Tyr Thr Ile Ile Asn Cys 
945                 950                 955                 960 


Met Gly Asn Asn Asn Ser Gly Trp Lys Ile Ser Leu Arg Thr Ile Arg 
                965                 970                 975     


Asp Cys Glu Ile Ile Trp Thr Leu Gln Asp Thr Ser Gly Asn Lys Glu 
            980                 985                 990         


Lys Leu Ile Phe Arg Tyr Glu Glu  Leu Ala Ser Ile Ser  Asp Tyr Ile 
        995                 1000                 1005             


Asn Lys  Trp Ile Phe Val Thr  Ile Thr Asn Asn Arg  Leu Gly Asn 
    1010                 1015                 1020             


Ser Arg  Ile Tyr Ile Asn Gly  Asn Leu Ile Val Glu  Lys Ser Ile 
    1025                 1030                 1035             


Ser Asn  Leu Gly Asp Ile His  Val Ser Asp Asn Ile  Leu Phe Lys 
    1040                 1045                 1050             


Ile Val  Gly Cys Asp Asp Glu  Thr Tyr Val Gly Ile  Arg Tyr Phe 
    1055                 1060                 1065             


Lys Val  Phe Asn Thr Glu Leu  Asp Lys Thr Glu Ile  Glu Thr Leu 
    1070                 1075                 1080             


Tyr Ser  Asn Glu Pro Asp Pro  Ser Ile Leu Lys Asp  Tyr Trp Gly 
    1085                 1090                 1095             


Asn Tyr  Leu Leu Tyr Asn Lys  Lys Tyr Tyr Leu Phe  Asn Leu Leu 
    1100                 1105                 1110             


Arg Lys  Asp Lys Tyr Ile Thr  Arg Asn Ser Gly Ile  Leu Asn Ile 
    1115                 1120                 1125             


Asn Gln  Gln Arg Gly Val Thr  Gly Gly Ile Ser Val  Phe Leu Asn 
    1130                 1135                 1140             


Tyr Lys  Leu Tyr Glu Gly Val  Glu Val Ile Ile Arg  Lys Asn Ala 
    1145                 1150                 1155             


Pro Ile  Asp Ile Ser Asn Thr  Asp Asn Phe Val Arg  Lys Asn Asp 
    1160                 1165                 1170             


Leu Ala  Tyr Ile Asn Val Val  Asp His Gly Val Glu  Tyr Arg Leu 
    1175                 1180                 1185             


Tyr Ala  Asp Ile Ser Ile Thr  Lys Ser Glu Lys Ile  Ile Lys Leu 
    1190                 1195                 1200             


Ile Arg  Thr Ser Asn Pro Asn  Asp Ser Leu Gly Gln  Ile Ile Val 
    1205                 1210                 1215             


Met Asp  Ser Ile Gly Asn Asn  Cys Thr Met Asn Phe  Gln Asn Asn 
    1220                 1225                 1230             


Asp Gly  Ser Asn Ile Gly Leu  Leu Gly Phe His Ser  Asp Asp Leu 
    1235                 1240                 1245             


Val Ala  Ser Ser Trp Tyr Tyr  Asn His Ile Arg Arg  Asn Thr Ser 
    1250                 1255                 1260             


Ser Asn  Gly Cys Phe Trp Ser  Phe Ile Ser Lys Glu  His Gly Trp 
    1265                 1270                 1275             


Lys Glu  
    1280     


<210>  13
<211>  3894
<212>  DNA
<213>  Clostridium botulinum


<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  n is a, c, g, or t

<400>  13
atgccagtta atataaaaan ctttaattat aatgacccta ttaataatga tgacattatt       60

atgatggaac cattcaatga cccagggcca ggaacatatt ataaagcttt taggattata      120

gatcgtattt ggatagtacc agaaaggttt acttatggat ttcaacctga ccaatttaat      180

gccagtacag gagtttttag taaagatgtc tacgaatatt acgatccaac ttatttaaaa      240

accgatgctg aaaaagataa atttttaaaa acaatgatta aattatttaa tagaattaat      300

tcaaaaccat caggacagag attactggat atgatagtag atgctatacc ttatcttgga      360

aatgcatcta caccgcccga caaatttgca gcaaatgttg caaatgtatc tattaataaa      420

aaaattatcc aacctggagc tgaagatcaa ataaaaggtt taatgacaaa tttaataata      480

tttggaccag gaccagttct aagtgataat tttactgata gtatgattat gaatggccat      540

tccccaatat cagaaggatt tggtgcaaga atgatgataa gattttgtcc tagttgttta      600

aatgtattta ataatgttca ggaaaataaa gatacatcta tatttagtag acgcgcgtat      660

tttgcagatc cagctctaac gttaatgcat gaacttatac atgtgttaca tggattatat      720

ggaattaaga taagtaattt accaattact ccaaatacaa aagaattttt catgcaacat      780

agcgatcctg tacaagcaga agaactatat acattcggag gacatgatcc tagtgttata      840

agtccttcta cggatatgaa tatttataat aaagcgttac aaaattttca agatatagct      900

aataggctta atattgtttc aagtgcccaa gggagtggaa ttgatatttc cttatataaa      960

caaatatata aaaataaata tgattttgtt gaagatccta atggaaaata tagtgtagat     1020

aaggataagt ttgataaatt atataaggcc ttaatgtttg gctttactga aactaatcta     1080

gctggtgaat atggaataaa aactaggtat tcttatttta gtgaatattt gccaccgata     1140

aaaactgaaa aattgttaga caatacaatt tatactcaaa atgaaggctt taacatagct     1200

agtaaaaatc tcaaaacgga atttaatggt cagaataagg cggtaaataa agaggcttat     1260

gaagaaatca gcctagaaca tctcgttata tatagaatag caatgtgcaa gcctgtaatg     1320

tacaaaaata ccggtaaatc tgaacagtgt attattgtta ataatgagga tttatttttc     1380

atagctaata aagatagttt ttcaaaagat ttagctaaag cagaaactat agcatataat     1440

acacaaaata atactataga aaataatttt tctatagatc agttgatttt agataatgat     1500

ttaagcagtg gcatagactt accaaatgaa aacacagaac catttacaaa ttttgacgac     1560

atagatatcc ctgtgtatat taaacaatct gctttaaaaa aaatttttgt ggatggagat     1620

agcctttttg aatatttaca tgctcaaaca tttccttcta atatagaaaa tctacaacta     1680

acgaattcat taaatgatgc tttaagaaat aataataaag tctatacttt tttttctaca     1740

aaccttgttg aaaaagctaa tacagttgta ggtgcttcac tttttgtaaa ctgggtaaaa     1800

ggagtaatag atgattttac atctgaatcc acacaaaaaa gtactataga taaagtttca     1860

gatgtatcca taattattcc ctatatagga cctgctttga atgtaggaaa tgaaacagct     1920

aaagaaaatt ttaaaaatgc ttttgaaata ggtggagccg ctatcttaat ggagtttatt     1980

ccagaactta ttgtacctat agttggattt tttacattag aatcatatgt aggaaataaa     2040

gggcatatta ttatgacgat atccaatgct ttaaagaaaa gggatcaaaa atggacagat     2100

atgtatggtt tgatagtatc gcagtggctc tcaacggtta atactcaatt ttatacaata     2160

aaagaaagaa tgtacaatgc tttaaataat caatcacaag caatagaaaa aataatagaa     2220

gatcaatata atagatatag tgaagaagat aaaatgaata ttaacattga ttttaatgat     2280

atagatttta aacttaatca aagtataaat ttagcaataa acaatataga tgattttata     2340

aaccaatgtt ctatatcata tctaatgaat agaatgattc cattagctgt aaaaaagtta     2400

aaagactttg atgataatct taagagagat ttattggagt atatagatac aaatgaacta     2460

tatttacttg atgaagtaaa tattctaaaa tcaaaagtaa atagacacct aaaagacagt     2520

ataccatttg atctttcact atataccaag gacacaattt taatacaagt ttttaataat     2580

tatattagta atattagtag taatgctatt ttaagtttaa gttatagagg tgggcgttta     2640

atagattcat ctggatatgg tgcaactatg aatgtaggtt cagatgttat ctttaatgat     2700

ataggaaatg gtcaatttaa attaaataat tctgaaaata gtaatattac ggcacatcaa     2760

agtaaattcg ttgtatatga tagtatgttt gataatttta gcattaactt ttgggtaagg     2820

actcctaaat ataataataa tgatatacaa acttatcttc aaaatgagta tacaataatt     2880

agttgtataa aaaatgactc aggatggaaa gtatctatta agggaaatag aataatatgg     2940

acattaatag atgttaatgc aaaatctaaa tcaatatttt tcgaatatag tataaaagat     3000

aatatatcag attatataaa taaatggttt tccataacta ttactaatga tagattaggt     3060

aacgcaaata tttatataaa tggaagtttg aaaaaaagtg aaaaaatttt aaacttagat     3120

agaattaatt ctagtaatga tatagacttc aaattaatta attgtacaga tactactaaa     3180

tttgtttgga ttaaggattt taatattttt ggtagagaat taaatgctac agaagtatct     3240

tcactatatt ggattcaatc atctacaaat actttaaaag atttttgggg gaatccttta     3300

agatacgata cacaatacta tctgtttaat caaggtatgc aaaatatcta tataaagtat     3360

tttagtaaag cttctatggg ggaaactgca ccacgtacaa actttaataa tgcagcaata     3420

aattatcaaa atttatatct tggtttacga tttattataa aaaaagcatc aaattctcgg     3480

aatataaata atgataatat agtcagagaa ggagattata tatatcttaa tattgataat     3540

atttctgatg aatcttacag agtatatgtt ttggtgaatt ctaaagaaat tcaaactcaa     3600

ttatttttag cacccataaa tgatgatcct acgttctatg atgtactaca aataaaaaaa     3660

tattatgaaa aaacaacata taattgtcag atactttgcg aaaaagatac taaaacattt     3720

gggctgtttg gaattggtaa atttgttaaa gattatggat atgtttggga tacctatgat     3780

aattattttt gcataagtca gtggtatctc agaagaatat ctgaaaatat aaataaatta     3840

aggttgggat gtaattggca attcattccc gtggatgaag gatggacaga ataa           3894


<210>  14
<211>  1297
<212>  PRT
<213>  Clostridium botulinum


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Xaa can be any naturally occurring amino acid

<400>  14

Met Pro Val Asn Ile Lys Xaa Phe Asn Tyr Asn Asp Pro Ile Asn Asn 
1               5                   10                  15      


Asp Asp Ile Ile Met Met Glu Pro Phe Asn Asp Pro Gly Pro Gly Thr 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Arg Ile Ile Asp Arg Ile Trp Ile Val Pro Glu 
        35                  40                  45              


Arg Phe Thr Tyr Gly Phe Gln Pro Asp Gln Phe Asn Ala Ser Thr Gly 
    50                  55                  60                  


Val Phe Ser Lys Asp Val Tyr Glu Tyr Tyr Asp Pro Thr Tyr Leu Lys 
65                  70                  75                  80  


Thr Asp Ala Glu Lys Asp Lys Phe Leu Lys Thr Met Ile Lys Leu Phe 
                85                  90                  95      


Asn Arg Ile Asn Ser Lys Pro Ser Gly Gln Arg Leu Leu Asp Met Ile 
            100                 105                 110         


Val Asp Ala Ile Pro Tyr Leu Gly Asn Ala Ser Thr Pro Pro Asp Lys 
        115                 120                 125             


Phe Ala Ala Asn Val Ala Asn Val Ser Ile Asn Lys Lys Ile Ile Gln 
    130                 135                 140                 


Pro Gly Ala Glu Asp Gln Ile Lys Gly Leu Met Thr Asn Leu Ile Ile 
145                 150                 155                 160 


Phe Gly Pro Gly Pro Val Leu Ser Asp Asn Phe Thr Asp Ser Met Ile 
                165                 170                 175     


Met Asn Gly His Ser Pro Ile Ser Glu Gly Phe Gly Ala Arg Met Met 
            180                 185                 190         


Ile Arg Phe Cys Pro Ser Cys Leu Asn Val Phe Asn Asn Val Gln Glu 
        195                 200                 205             


Asn Lys Asp Thr Ser Ile Phe Ser Arg Arg Ala Tyr Phe Ala Asp Pro 
    210                 215                 220                 


Ala Leu Thr Leu Met His Glu Leu Ile His Val Leu His Gly Leu Tyr 
225                 230                 235                 240 


Gly Ile Lys Ile Ser Asn Leu Pro Ile Thr Pro Asn Thr Lys Glu Phe 
                245                 250                 255     


Phe Met Gln His Ser Asp Pro Val Gln Ala Glu Glu Leu Tyr Thr Phe 
            260                 265                 270         


Gly Gly His Asp Pro Ser Val Ile Ser Pro Ser Thr Asp Met Asn Ile 
        275                 280                 285             


Tyr Asn Lys Ala Leu Gln Asn Phe Gln Asp Ile Ala Asn Arg Leu Asn 
    290                 295                 300                 


Ile Val Ser Ser Ala Gln Gly Ser Gly Ile Asp Ile Ser Leu Tyr Lys 
305                 310                 315                 320 


Gln Ile Tyr Lys Asn Lys Tyr Asp Phe Val Glu Asp Pro Asn Gly Lys 
                325                 330                 335     


Tyr Ser Val Asp Lys Asp Lys Phe Asp Lys Leu Tyr Lys Ala Leu Met 
            340                 345                 350         


Phe Gly Phe Thr Glu Thr Asn Leu Ala Gly Glu Tyr Gly Ile Lys Thr 
        355                 360                 365             


Arg Tyr Ser Tyr Phe Ser Glu Tyr Leu Pro Pro Ile Lys Thr Glu Lys 
    370                 375                 380                 


Leu Leu Asp Asn Thr Ile Tyr Thr Gln Asn Glu Gly Phe Asn Ile Ala 
385                 390                 395                 400 


Ser Lys Asn Leu Lys Thr Glu Phe Asn Gly Gln Asn Lys Ala Val Asn 
                405                 410                 415     


Lys Glu Ala Tyr Glu Glu Ile Ser Leu Glu His Leu Val Ile Tyr Arg 
            420                 425                 430         


Ile Ala Met Cys Lys Pro Val Met Tyr Lys Asn Thr Gly Lys Ser Glu 
        435                 440                 445             


Gln Cys Ile Ile Val Asn Asn Glu Asp Leu Phe Phe Ile Ala Asn Lys 
    450                 455                 460                 


Asp Ser Phe Ser Lys Asp Leu Ala Lys Ala Glu Thr Ile Ala Tyr Asn 
465                 470                 475                 480 


Thr Gln Asn Asn Thr Ile Glu Asn Asn Phe Ser Ile Asp Gln Leu Ile 
                485                 490                 495     


Leu Asp Asn Asp Leu Ser Ser Gly Ile Asp Leu Pro Asn Glu Asn Thr 
            500                 505                 510         


Glu Pro Phe Thr Asn Phe Asp Asp Ile Asp Ile Pro Val Tyr Ile Lys 
        515                 520                 525             


Gln Ser Ala Leu Lys Lys Ile Phe Val Asp Gly Asp Ser Leu Phe Glu 
    530                 535                 540                 


Tyr Leu His Ala Gln Thr Phe Pro Ser Asn Ile Glu Asn Leu Gln Leu 
545                 550                 555                 560 


Thr Asn Ser Leu Asn Asp Ala Leu Arg Asn Asn Asn Lys Val Tyr Thr 
                565                 570                 575     


Phe Phe Ser Thr Asn Leu Val Glu Lys Ala Asn Thr Val Val Gly Ala 
            580                 585                 590         


Ser Leu Phe Val Asn Trp Val Lys Gly Val Ile Asp Asp Phe Thr Ser 
        595                 600                 605             


Glu Ser Thr Gln Lys Ser Thr Ile Asp Lys Val Ser Asp Val Ser Ile 
    610                 615                 620                 


Ile Ile Pro Tyr Ile Gly Pro Ala Leu Asn Val Gly Asn Glu Thr Ala 
625                 630                 635                 640 


Lys Glu Asn Phe Lys Asn Ala Phe Glu Ile Gly Gly Ala Ala Ile Leu 
                645                 650                 655     


Met Glu Phe Ile Pro Glu Leu Ile Val Pro Ile Val Gly Phe Phe Thr 
            660                 665                 670         


Leu Glu Ser Tyr Val Gly Asn Lys Gly His Ile Ile Met Thr Ile Ser 
        675                 680                 685             


Asn Ala Leu Lys Lys Arg Asp Gln Lys Trp Thr Asp Met Tyr Gly Leu 
    690                 695                 700                 


Ile Val Ser Gln Trp Leu Ser Thr Val Asn Thr Gln Phe Tyr Thr Ile 
705                 710                 715                 720 


Lys Glu Arg Met Tyr Asn Ala Leu Asn Asn Gln Ser Gln Ala Ile Glu 
                725                 730                 735     


Lys Ile Ile Glu Asp Gln Tyr Asn Arg Tyr Ser Glu Glu Asp Lys Met 
            740                 745                 750         


Asn Ile Asn Ile Asp Phe Asn Asp Ile Asp Phe Lys Leu Asn Gln Ser 
        755                 760                 765             


Ile Asn Leu Ala Ile Asn Asn Ile Asp Asp Phe Ile Asn Gln Cys Ser 
    770                 775                 780                 


Ile Ser Tyr Leu Met Asn Arg Met Ile Pro Leu Ala Val Lys Lys Leu 
785                 790                 795                 800 


Lys Asp Phe Asp Asp Asn Leu Lys Arg Asp Leu Leu Glu Tyr Ile Asp 
                805                 810                 815     


Thr Asn Glu Leu Tyr Leu Leu Asp Glu Val Asn Ile Leu Lys Ser Lys 
            820                 825                 830         


Val Asn Arg His Leu Lys Asp Ser Ile Pro Phe Asp Leu Ser Leu Tyr 
        835                 840                 845             


Thr Lys Asp Thr Ile Leu Ile Gln Val Phe Asn Asn Tyr Ile Ser Asn 
    850                 855                 860                 


Ile Ser Ser Asn Ala Ile Leu Ser Leu Ser Tyr Arg Gly Gly Arg Leu 
865                 870                 875                 880 


Ile Asp Ser Ser Gly Tyr Gly Ala Thr Met Asn Val Gly Ser Asp Val 
                885                 890                 895     


Ile Phe Asn Asp Ile Gly Asn Gly Gln Phe Lys Leu Asn Asn Ser Glu 
            900                 905                 910         


Asn Ser Asn Ile Thr Ala His Gln Ser Lys Phe Val Val Tyr Asp Ser 
        915                 920                 925             


Met Phe Asp Asn Phe Ser Ile Asn Phe Trp Val Arg Thr Pro Lys Tyr 
    930                 935                 940                 


Asn Asn Asn Asp Ile Gln Thr Tyr Leu Gln Asn Glu Tyr Thr Ile Ile 
945                 950                 955                 960 


Ser Cys Ile Lys Asn Asp Ser Gly Trp Lys Val Ser Ile Lys Gly Asn 
                965                 970                 975     


Arg Ile Ile Trp Thr Leu Ile Asp Val Asn Ala Lys Ser Lys Ser Ile 
            980                 985                 990         


Phe Phe Glu Tyr Ser Ile Lys Asp  Asn Ile Ser Asp Tyr  Ile Asn Lys 
        995                 1000                 1005             


Trp Phe  Ser Ile Thr Ile Thr  Asn Asp Arg Leu Gly  Asn Ala Asn 
    1010                 1015                 1020             


Ile Tyr  Ile Asn Gly Ser Leu  Lys Lys Ser Glu Lys  Ile Leu Asn 
    1025                 1030                 1035             


Leu Asp  Arg Ile Asn Ser Ser  Asn Asp Ile Asp Phe  Lys Leu Ile 
    1040                 1045                 1050             


Asn Cys  Thr Asp Thr Thr Lys  Phe Val Trp Ile Lys  Asp Phe Asn 
    1055                 1060                 1065             


Ile Phe  Gly Arg Glu Leu Asn  Ala Thr Glu Val Ser  Ser Leu Tyr 
    1070                 1075                 1080             


Trp Ile  Gln Ser Ser Thr Asn  Thr Leu Lys Asp Phe  Trp Gly Asn 
    1085                 1090                 1095             


Pro Leu  Arg Tyr Asp Thr Gln  Tyr Tyr Leu Phe Asn  Gln Gly Met 
    1100                 1105                 1110             


Gln Asn  Ile Tyr Ile Lys Tyr  Phe Ser Lys Ala Ser  Met Gly Glu 
    1115                 1120                 1125             


Thr Ala  Pro Arg Thr Asn Phe  Asn Asn Ala Ala Ile  Asn Tyr Gln 
    1130                 1135                 1140             


Asn Leu  Tyr Leu Gly Leu Arg  Phe Ile Ile Lys Lys  Ala Ser Asn 
    1145                 1150                 1155             


Ser Arg  Asn Ile Asn Asn Asp  Asn Ile Val Arg Glu  Gly Asp Tyr 
    1160                 1165                 1170             


Ile Tyr  Leu Asn Ile Asp Asn  Ile Ser Asp Glu Ser  Tyr Arg Val 
    1175                 1180                 1185             


Tyr Val  Leu Val Asn Ser Lys  Glu Ile Gln Thr Gln  Leu Phe Leu 
    1190                 1195                 1200             


Ala Pro  Ile Asn Asp Asp Pro  Thr Phe Tyr Asp Val  Leu Gln Ile 
    1205                 1210                 1215             


Lys Lys  Tyr Tyr Glu Lys Thr  Thr Tyr Asn Cys Gln  Ile Leu Cys 
    1220                 1225                 1230             


Glu Lys  Asp Thr Lys Thr Phe  Gly Leu Phe Gly Ile  Gly Lys Phe 
    1235                 1240                 1245             


Val Lys  Asp Tyr Gly Tyr Val  Trp Asp Thr Tyr Asp  Asn Tyr Phe 
    1250                 1255                 1260             


Cys Ile  Ser Gln Trp Tyr Leu  Arg Arg Ile Ser Glu  Asn Ile Asn 
    1265                 1270                 1275             


Lys Leu  Arg Leu Gly Cys Asn  Trp Gln Phe Ile Pro  Val Asp Glu 
    1280                 1285                 1290             


Gly Trp  Thr Glu 
    1295         


<210>  15
<211>  4400
<212>  DNA
<213>  Clostridium tetani

<400>  15
tagcattaaa aaaattagaa cctatagtaa ataaattaat taatatatag tttttataat       60

ttaattatga ataatattct taagataaaa agtaaatttt taaaaattta aattttcagt      120

ttacaaaaaa taacctgatt atgttatatg taattgtaaa aaacatataa aaaatcagaa      180

aaatttagga ggtatattat taatggatta aataataatt ttttaattta cttttgatta      240

ataaatatta aatgtttatt ttaattagga gatgatacgt atgccaataa ccataaataa      300

ttttagatat agtgatcctg ttaataatga tacaattatt atgatggagc caccatactg      360

taagggtcta gatatctatt ataaggcttt caaaataaca gatcgtattt ggatagtgcc      420

ggaaaggtat gaatttggga caaaacctga agattttaac ccaccatctt cattaataga      480

aggtgcatct gagtattacg atccaaatta tttaaggact gattctgata aagatagatt      540

tttacaaacc atggtaaaac tgtttaacag aattaaaaac aatgtagcag gtgaagcctt      600

attagataag ataataaatg ccatacctta ccttggaaat tcatattcct tactagacaa      660

gtttgataca aactctaatt cagtatcttt taatttatta gaacaagacc ccagtggagc      720

aactacaaaa tcagcaatgc tgacaaattt aataatattt ggacctgggc ctgttttaaa      780

taaaaatgag gttagaggta ttgtattgag ggtagataat aaaaattact tcccatgtag      840

agatggtttt ggctcaataa tgcaaatggc attttgccca gaatatgtac ctacctttga      900

taatgtaata gaaaatatta cgtcactcac tattggcaaa agcaaatatt ttcaagatcc      960

agcattacta ttaatgcacg aacttataca tgtactacat ggtttatacg gaatgcaggt     1020

atcaagccat gaaattattc catccaaaca agaaatttat atgcagcata catatccaat     1080

aagtgctgaa gaactattca cttttggcgg acaggatgct aatcttataa gtattgatat     1140

aaaaaacgat ttatatgaaa aaactttaaa tgattataaa gctatagcta acaaacttag     1200

tcaagtcact agctgcaatg atcccaacat tgatattgat agctacaaac aaatatatca     1260

acaaaaatat caattcgata aagatagcaa tggacaatat attgtaaatg aggataaatt     1320

tcagatacta tataatagca taatgtatgg ttttacagag attgaattgg gaaaaaaatt     1380

taatataaaa actagacttt cttattttag tatgaatcat gaccctgtaa aaattccaaa     1440

tttattagat gatacaattt acaatgatac agaaggattt aatatagaaa gcaaagatct     1500

gaaatctgaa tataaaggac aaaatatgag ggtaaataca aatgctttta gaaatgttga     1560

tggatcaggc ctagtttcaa aacttattgg cttatgtaaa aaaattatac caccaacaaa     1620

tataagagaa aatttatata atagaactgc atcattaaca gatttaggag gagaattatg     1680

tataaaaatt aaaaatgaag atttaacttt tatagctgaa aaaaatagct tttcagaaga     1740

accatttcaa gatgaaatag ttagttataa tacaaaaaat aaaccattaa attttaatta     1800

ttcgctagat aaaattattg tagattataa tctacaaagt aaaattacat tacctaatga     1860

taggacaacc ccagttacaa aaggaattcc atatgctcca gaatataaaa gtaatgctgc     1920

aagtacaata gaaatacata atattgatga caatacaata tatcaatatt tgtatgctca     1980

aaaatctcct acaactctac aaagaataac tatgactaat tctgttgatg acgcattaat     2040

aaattccacc aaaatatatt catattttcc atctgtaatc agtaaagtta accaaggtgc     2100

acaaggaatt ttattcttac agtgggtgag agatataatt gatgatttta ccaatgaatc     2160

ttcacaaaaa actactattg ataaaatttc agatgtatcc actattgttc cttatatagg     2220

acccgcatta aacattgtaa aacaaggcta tgagggaaac tttataggcg ctttagaaac     2280

taccggagtg gttttattat tagaatatat tccagaaatt actttaccag taattgcagc     2340

tttatctata gcagaaagta gcacacaaaa agaaaagata ataaaaacaa tagataactt     2400

tttagaaaaa agatatgaaa aatggattga agtatataaa ctagtaaaag caaaatggtt     2460

aggcacagtt aatacgcaat tccaaaaaag aagttatcaa atgtatagat ctttagaata     2520

tcaagtagat gcaataaaaa aaataataga ctatgaatat aaaatatatt caggacctga     2580

taaggaacaa attgccgacg aaattaataa tctgaaaaac aaacttgaag aaaaggctaa     2640

taaagcaatg ataaacataa atatatttat gagggaaagt tctagatcat ttttagttaa     2700

tcaaatgatt aacgaagcta aaaagcagtt attagagttt gatactcaaa gcaaaaatat     2760

tttaatgcag tatataaaag caaattctaa atttataggt ataactgaac taaaaaaatt     2820

agaatcaaaa ataaacaaag ttttttcaac accaattcca ttttcttatt ctaaaaatct     2880

ggattgttgg gttgataatg aagaagatat agatgttata ttaaaaaaga gtacaatttt     2940

aaatttagat attaataatg atattatatc agatatatct gggtttaatt catctgtaat     3000

aacatatcca gatgctcaat tggtgcccgg aataaatggc aaagcaatac atttagtaaa     3060

caatgaatct tctgaagtta tagtgcataa agctatggat attgaatata atgatatgtt     3120

taataatttt accgttagct tttggttgag ggttcctaaa gtatctgcta gtcatttaga     3180

acaatatggc acaaatgagt attcaataat tagctctatg aaaaaacata gtctatcaat     3240

aggatctggt tggagtgtat cacttaaagg taataactta atatggactt taaaagattc     3300

cgcgggagaa gttagacaaa taacttttag ggatttacct gataaattta atgcttattt     3360

agcaaataaa tgggttttta taactattac taatgataga ttatcttctg ctaatttgta     3420

tataaatgga gtacttatgg gaagtgcaga aattactggt ttaggagcta ttagagagga     3480

taataatata acattaaaac tagatagatg taataataat aatcaatacg tttctattga     3540

taaatttagg atattttgca aagcattaaa tccaaaagag attgaaaaat tatacacaag     3600

ttatttatct ataacctttt taagagactt ctggggaaac cctttacgat atgatacaga     3660

atattattta ataccagtag cttctagttc taaagatgtt caattgaaaa atataacaga     3720

ttatatgtat ttgacaaatg cgccatcgta tactaacgga aaattgaata tatattatag     3780

aaggttatat aatggactaa aatttattat aaaaagatat acacctaata atgaaataga     3840

ttcttttgtt aaatcaggtg attttattaa attatatgta tcatataaca ataatgagca     3900

cattgtaggt tatccgaaag atggaaatgc ctttaataat cttgatagaa ttctaagagt     3960

aggttataat gccccaggta tccctcttta taaaaaaatg gaagcagtaa aattgcgtga     4020

tttaaaaacc tattctgtac aacttaaatt atatgatgat aaaaatgcat ctttaggact     4080

agtaggtacc cataatggtc aaataggcaa cgatccaaat agggatatat taattgcaag     4140

caactggtac tttaatcatt taaaagataa aattttagga tgtgattggt actttgtacc     4200

tacagatgaa ggatggacaa atgattaaac agattgatat gttcatgatt actctatata     4260

aaaaattaaa taatataaca atctagctat attatttttg attattttct taatatatac     4320

taataaaata atcaaaatag agcctatctt aaattactga agggctgtgt caaaataaga     4380

ttttgacaca gcctctactt                                                 4400


<210>  16
<211>  1315
<212>  PRT
<213>  Clostridium tetani

<400>  16

Met Pro Ile Thr Ile Asn Asn Phe Arg Tyr Ser Asp Pro Val Asn Asn 
1               5                   10                  15      


Asp Thr Ile Ile Met Met Glu Pro Pro Tyr Cys Lys Gly Leu Asp Ile 
            20                  25                  30          


Tyr Tyr Lys Ala Phe Lys Ile Thr Asp Arg Ile Trp Ile Val Pro Glu 
        35                  40                  45              


Arg Tyr Glu Phe Gly Thr Lys Pro Glu Asp Phe Asn Pro Pro Ser Ser 
    50                  55                  60                  


Leu Ile Glu Gly Ala Ser Glu Tyr Tyr Asp Pro Asn Tyr Leu Arg Thr 
65                  70                  75                  80  


Asp Ser Asp Lys Asp Arg Phe Leu Gln Thr Met Val Lys Leu Phe Asn 
                85                  90                  95      


Arg Ile Lys Asn Asn Val Ala Gly Glu Ala Leu Leu Asp Lys Ile Ile 
            100                 105                 110         


Asn Ala Ile Pro Tyr Leu Gly Asn Ser Tyr Ser Leu Leu Asp Lys Phe 
        115                 120                 125             


Asp Thr Asn Ser Asn Ser Val Ser Phe Asn Leu Leu Glu Gln Asp Pro 
    130                 135                 140                 


Ser Gly Ala Thr Thr Lys Ser Ala Met Leu Thr Asn Leu Ile Ile Phe 
145                 150                 155                 160 


Gly Pro Gly Pro Val Leu Asn Lys Asn Glu Val Arg Gly Ile Val Leu 
                165                 170                 175     


Arg Val Asp Asn Lys Asn Tyr Phe Pro Cys Arg Asp Gly Phe Gly Ser 
            180                 185                 190         


Ile Met Gln Met Ala Phe Cys Pro Glu Tyr Val Pro Thr Phe Asp Asn 
        195                 200                 205             


Val Ile Glu Asn Ile Thr Ser Leu Thr Ile Gly Lys Ser Lys Tyr Phe 
    210                 215                 220                 


Gln Asp Pro Ala Leu Leu Leu Met His Glu Leu Ile His Val Leu His 
225                 230                 235                 240 


Gly Leu Tyr Gly Met Gln Val Ser Ser His Glu Ile Ile Pro Ser Lys 
                245                 250                 255     


Gln Glu Ile Tyr Met Gln His Thr Tyr Pro Ile Ser Ala Glu Glu Leu 
            260                 265                 270         


Phe Thr Phe Gly Gly Gln Asp Ala Asn Leu Ile Ser Ile Asp Ile Lys 
        275                 280                 285             


Asn Asp Leu Tyr Glu Lys Thr Leu Asn Asp Tyr Lys Ala Ile Ala Asn 
    290                 295                 300                 


Lys Leu Ser Gln Val Thr Ser Cys Asn Asp Pro Asn Ile Asp Ile Asp 
305                 310                 315                 320 


Ser Tyr Lys Gln Ile Tyr Gln Gln Lys Tyr Gln Phe Asp Lys Asp Ser 
                325                 330                 335     


Asn Gly Gln Tyr Ile Val Asn Glu Asp Lys Phe Gln Ile Leu Tyr Asn 
            340                 345                 350         


Ser Ile Met Tyr Gly Phe Thr Glu Ile Glu Leu Gly Lys Lys Phe Asn 
        355                 360                 365             


Ile Lys Thr Arg Leu Ser Tyr Phe Ser Met Asn His Asp Pro Val Lys 
    370                 375                 380                 


Ile Pro Asn Leu Leu Asp Asp Thr Ile Tyr Asn Asp Thr Glu Gly Phe 
385                 390                 395                 400 


Asn Ile Glu Ser Lys Asp Leu Lys Ser Glu Tyr Lys Gly Gln Asn Met 
                405                 410                 415     


Arg Val Asn Thr Asn Ala Phe Arg Asn Val Asp Gly Ser Gly Leu Val 
            420                 425                 430         


Ser Lys Leu Ile Gly Leu Cys Lys Lys Ile Ile Pro Pro Thr Asn Ile 
        435                 440                 445             


Arg Glu Asn Leu Tyr Asn Arg Thr Ala Ser Leu Thr Asp Leu Gly Gly 
    450                 455                 460                 


Glu Leu Cys Ile Lys Ile Lys Asn Glu Asp Leu Thr Phe Ile Ala Glu 
465                 470                 475                 480 


Lys Asn Ser Phe Ser Glu Glu Pro Phe Gln Asp Glu Ile Val Ser Tyr 
                485                 490                 495     


Asn Thr Lys Asn Lys Pro Leu Asn Phe Asn Tyr Ser Leu Asp Lys Ile 
            500                 505                 510         


Ile Val Asp Tyr Asn Leu Gln Ser Lys Ile Thr Leu Pro Asn Asp Arg 
        515                 520                 525             


Thr Thr Pro Val Thr Lys Gly Ile Pro Tyr Ala Pro Glu Tyr Lys Ser 
    530                 535                 540                 


Asn Ala Ala Ser Thr Ile Glu Ile His Asn Ile Asp Asp Asn Thr Ile 
545                 550                 555                 560 


Tyr Gln Tyr Leu Tyr Ala Gln Lys Ser Pro Thr Thr Leu Gln Arg Ile 
                565                 570                 575     


Thr Met Thr Asn Ser Val Asp Asp Ala Leu Ile Asn Ser Thr Lys Ile 
            580                 585                 590         


Tyr Ser Tyr Phe Pro Ser Val Ile Ser Lys Val Asn Gln Gly Ala Gln 
        595                 600                 605             


Gly Ile Leu Phe Leu Gln Trp Val Arg Asp Ile Ile Asp Asp Phe Thr 
    610                 615                 620                 


Asn Glu Ser Ser Gln Lys Thr Thr Ile Asp Lys Ile Ser Asp Val Ser 
625                 630                 635                 640 


Thr Ile Val Pro Tyr Ile Gly Pro Ala Leu Asn Ile Val Lys Gln Gly 
                645                 650                 655     


Tyr Glu Gly Asn Phe Ile Gly Ala Leu Glu Thr Thr Gly Val Val Leu 
            660                 665                 670         


Leu Leu Glu Tyr Ile Pro Glu Ile Thr Leu Pro Val Ile Ala Ala Leu 
        675                 680                 685             


Ser Ile Ala Glu Ser Ser Thr Gln Lys Glu Lys Ile Ile Lys Thr Ile 
    690                 695                 700                 


Asp Asn Phe Leu Glu Lys Arg Tyr Glu Lys Trp Ile Glu Val Tyr Lys 
705                 710                 715                 720 


Leu Val Lys Ala Lys Trp Leu Gly Thr Val Asn Thr Gln Phe Gln Lys 
                725                 730                 735     


Arg Ser Tyr Gln Met Tyr Arg Ser Leu Glu Tyr Gln Val Asp Ala Ile 
            740                 745                 750         


Lys Lys Ile Ile Asp Tyr Glu Tyr Lys Ile Tyr Ser Gly Pro Asp Lys 
        755                 760                 765             


Glu Gln Ile Ala Asp Glu Ile Asn Asn Leu Lys Asn Lys Leu Glu Glu 
    770                 775                 780                 


Lys Ala Asn Lys Ala Met Ile Asn Ile Asn Ile Phe Met Arg Glu Ser 
785                 790                 795                 800 


Ser Arg Ser Phe Leu Val Asn Gln Met Ile Asn Glu Ala Lys Lys Gln 
                805                 810                 815     


Leu Leu Glu Phe Asp Thr Gln Ser Lys Asn Ile Leu Met Gln Tyr Ile 
            820                 825                 830         


Lys Ala Asn Ser Lys Phe Ile Gly Ile Thr Glu Leu Lys Lys Leu Glu 
        835                 840                 845             


Ser Lys Ile Asn Lys Val Phe Ser Thr Pro Ile Pro Phe Ser Tyr Ser 
    850                 855                 860                 


Lys Asn Leu Asp Cys Trp Val Asp Asn Glu Glu Asp Ile Asp Val Ile 
865                 870                 875                 880 


Leu Lys Lys Ser Thr Ile Leu Asn Leu Asp Ile Asn Asn Asp Ile Ile 
                885                 890                 895     


Ser Asp Ile Ser Gly Phe Asn Ser Ser Val Ile Thr Tyr Pro Asp Ala 
            900                 905                 910         


Gln Leu Val Pro Gly Ile Asn Gly Lys Ala Ile His Leu Val Asn Asn 
        915                 920                 925             


Glu Ser Ser Glu Val Ile Val His Lys Ala Met Asp Ile Glu Tyr Asn 
    930                 935                 940                 


Asp Met Phe Asn Asn Phe Thr Val Ser Phe Trp Leu Arg Val Pro Lys 
945                 950                 955                 960 


Val Ser Ala Ser His Leu Glu Gln Tyr Gly Thr Asn Glu Tyr Ser Ile 
                965                 970                 975     


Ile Ser Ser Met Lys Lys His Ser Leu Ser Ile Gly Ser Gly Trp Ser 
            980                 985                 990         


Val Ser Leu Lys Gly Asn Asn Leu  Ile Trp Thr Leu Lys  Asp Ser Ala 
        995                 1000                 1005             


Gly Glu  Val Arg Gln Ile Thr  Phe Arg Asp Leu Pro  Asp Lys Phe 
    1010                 1015                 1020             


Asn Ala  Tyr Leu Ala Asn Lys  Trp Val Phe Ile Thr  Ile Thr Asn 
    1025                 1030                 1035             


Asp Arg  Leu Ser Ser Ala Asn  Leu Tyr Ile Asn Gly  Val Leu Met 
    1040                 1045                 1050             


Gly Ser  Ala Glu Ile Thr Gly  Leu Gly Ala Ile Arg  Glu Asp Asn 
    1055                 1060                 1065             


Asn Ile  Thr Leu Lys Leu Asp  Arg Cys Asn Asn Asn  Asn Gln Tyr 
    1070                 1075                 1080             


Val Ser  Ile Asp Lys Phe Arg  Ile Phe Cys Lys Ala  Leu Asn Pro 
    1085                 1090                 1095             


Lys Glu  Ile Glu Lys Leu Tyr  Thr Ser Tyr Leu Ser  Ile Thr Phe 
    1100                 1105                 1110             


Leu Arg  Asp Phe Trp Gly Asn  Pro Leu Arg Tyr Asp  Thr Glu Tyr 
    1115                 1120                 1125             


Tyr Leu  Ile Pro Val Ala Ser  Ser Ser Lys Asp Val  Gln Leu Lys 
    1130                 1135                 1140             


Asn Ile  Thr Asp Tyr Met Tyr  Leu Thr Asn Ala Pro  Ser Tyr Thr 
    1145                 1150                 1155             


Asn Gly  Lys Leu Asn Ile Tyr  Tyr Arg Arg Leu Tyr  Asn Gly Leu 
    1160                 1165                 1170             


Lys Phe  Ile Ile Lys Arg Tyr  Thr Pro Asn Asn Glu  Ile Asp Ser 
    1175                 1180                 1185             


Phe Val  Lys Ser Gly Asp Phe  Ile Lys Leu Tyr Val  Ser Tyr Asn 
    1190                 1195                 1200             


Asn Asn  Glu His Ile Val Gly  Tyr Pro Lys Asp Gly  Asn Ala Phe 
    1205                 1210                 1215             


Asn Asn  Leu Asp Arg Ile Leu  Arg Val Gly Tyr Asn  Ala Pro Gly 
    1220                 1225                 1230             


Ile Pro  Leu Tyr Lys Lys Met  Glu Ala Val Lys Leu  Arg Asp Leu 
    1235                 1240                 1245             


Lys Thr  Tyr Ser Val Gln Leu  Lys Leu Tyr Asp Asp  Lys Asn Ala 
    1250                 1255                 1260             


Ser Leu  Gly Leu Val Gly Thr  His Asn Gly Gln Ile  Gly Asn Asp 
    1265                 1270                 1275             


Pro Asn  Arg Asp Ile Leu Ile  Ala Ser Asn Trp Tyr  Phe Asn His 
    1280                 1285                 1290             


Leu Lys  Asp Lys Ile Leu Gly  Cys Asp Trp Tyr Phe  Val Pro Thr 
    1295                 1300                 1305             


Asp Glu  Gly Trp Thr Asn Asp  
    1310                 1315 



