                         SEQUENCE LISTING

<110>  MASSACHUSETTS INSTITUTE OF TECHNOLOGY
       Sasisekharan, Ram
       Robinson, Luke N
       Tharakaraman, Kannan
 
<120>  Novel, full spectrum anti-dengue antibody

<130>  0492611-1258

<160>  44    

<170>  PatentIn version 3.5

<210>  1
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  wt 4E11 HC

<400>  1

Glu Val Lys Leu Leu Glu Gln Ser Gly Ala Glu Leu Val Lys Pro Gly 
1               5                   10                  15      


Ala Ser Val Arg Leu Ser Cys Thr Ala Ser Gly Phe Asn Ile Lys Asp 
            20                  25                  30          


Thr Tyr Met Ser Trp Val Lys Gln Arg Pro Glu Gln Gly Leu Glu Trp 
        35                  40                  45              


Ile Gly Arg Ile Asp Pro Ala Asn Gly Asp Thr Lys Tyr Asp Pro Lys 
    50                  55                  60                  


Phe Gln Gly Lys Ala Thr Ile Thr Ala Asp Thr Ser Ser Asn Thr Ala 
65                  70                  75                  80  


Tyr Leu His Leu Ser Ser Leu Thr Ser Gly Asp Thr Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ser Arg Gly Trp Glu Gly Phe Ala Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ala 
        115         


<210>  2
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  wt 4E11 LC

<400>  2

Glu Leu Val Met Thr Gln Thr Pro Ala Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Asn Val Asp Arg Tyr 
            20                  25                  30          


Gly Asn Ser Phe Met His Trp Tyr Gln Gln Lys Ala Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Arg Ala Ser Asn Leu Glu Ser Gly Ile Pro Ala 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Arg Thr Asp Phe Thr Leu Thr Ile Asn 
65                  70                  75                  80  


Pro Val Glu Ala Asp Asp Val Ala Thr Tyr Phe Cys Gln Arg Ser Asn 
                85                  90                  95      


Glu Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
            100                 105                 110         


<210>  3
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  wt 4E11 HC FR1

<400>  3

Glu Val Lys Leu Leu Glu Gln Ser Gly Ala Glu Leu Val Lys Pro Gly 
1               5                   10                  15      


Ala Ser Val Arg Leu Ser Cys Thr Ala Ser 
            20                  25      


<210>  4
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  wt 4E11 HC FR2

<400>  4

Tyr Met Ser Trp Val Lys Gln Arg Pro Glu Gln Gly Leu Glu Trp Ile 
1               5                   10                  15      


Gly Arg Ile 
            


<210>  5
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  wt 4E11 HC FR3

<400>  5

Thr Lys Tyr Asp Pro Lys Phe Gln Gly Lys Ala Thr Ile Thr Ala Asp 
1               5                   10                  15      


Thr Ser Ser Asn Thr Ala Tyr Leu His Leu Ser Ser Leu Thr Ser Gly 
            20                  25                  30          


Asp Thr Ala Val Tyr Tyr Cys Ser Arg 
        35                  40      


<210>  6
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  wt 4E11 HC FR4

<400>  6

Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala 
1               5                   10      


<210>  7
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  wt 4E11 HC CDR1

<400>  7

Gly Phe Asn Ile Lys Asp Thr 
1               5           


<210>  8
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  wt 4E11 HC CDR2

<400>  8

Asp Pro Ala Asn Gly Asp 
1               5       


<210>  9
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  wt 4E11 HC CDR3

<400>  9

Gly Trp Glu Gly Phe Ala Tyr 
1               5           


<210>  10
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  wt 4E11 LC FR1

<400>  10

Glu Leu Val Met Thr Gln Thr Pro Ala Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Gln Arg Ala Thr Ile Ser Cys 
            20              


<210>  11
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  wt 4E11 LC FR2

<400>  11

Trp Tyr Gln Gln Lys Ala Gly Gln Pro Pro Lys Leu Leu Ile Tyr 
1               5                   10                  15  


<210>  12
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  wt 4E11 LC FR3

<400>  12

Gly Ile Pro Ala Arg Phe Ser Gly Ser Gly Ser Arg Thr Asp Phe Thr 
1               5                   10                  15      


Leu Thr Ile Asn Pro Val Glu Ala Asp Asp Val Ala Thr Tyr Phe Cys 
            20                  25                  30          


<210>  13
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  wt 4E11 LC FR4

<400>  13

Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
1               5                   10      


<210>  14
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  wt 4E11 LC CDR1

<400>  14

Arg Ala Ser Glu Asn Val Asp Arg Tyr Gly Asn Ser Phe Met His 
1               5                   10                  15  


<210>  15
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  wt 4E11 LC CDR2

<400>  15

Arg Ala Ser Asn Leu Glu Ser 
1               5           


<210>  16
<211>  9
<212>  PRT
<213>  artificial

<220>
<223>  wt 4E11 LC CDR3

<400>  16

Gln Arg Ser Asn Glu Val Pro Trp Thr 
1               5                   


<210>  17
<211>  93
<212>  PRT
<213>  Dengue virus type 1

<400>  17

Met Cys Thr Gly Ser Phe Lys Leu Glu Lys Glu Val Ala Glu Thr Gln 
1               5                   10                  15      


His Gly Thr Val Leu Val Gln Val Lys Tyr Glu Gly Thr Asp Ala Pro 
            20                  25                  30          


Cys Lys Ile Pro Phe Ser Ser Gln Asp Glu Lys Gly Val Thr Gln Asn 
        35                  40                  45              


Gly Arg Leu Ile Thr Ala Asn Pro Ile Val Thr Asp Lys Glu Lys Pro 
    50                  55                  60                  


Val Asn Ile Glu Ala Glu Pro Pro Phe Gly Glu Ser Tyr Ile Val Val 
65                  70                  75                  80  


Gly Ala Gly Glu Lys Ala Leu Lys Leu Ser Trp Phe Lys 
                85                  90              


<210>  18
<211>  93
<212>  PRT
<213>  Dengue virus type 2

<400>  18

Met Cys Thr Gly Lys Phe Lys Val Val Lys Glu Ile Ala Glu Thr Gln 
1               5                   10                  15      


His Gly Thr Met Val Ile Arg Val Gln Tyr Glu Gly Asp Asp Ser Pro 
            20                  25                  30          


Cys Lys Ile Pro Phe Glu Ile Met Asp Leu Glu Lys Lys His Val Leu 
        35                  40                  45              


Gly Arg Leu Ile Thr Val Asn Pro Ile Val Ile Glu Lys Asp Ser Pro 
    50                  55                  60                  


Ile Asn Ile Glu Ala Glu Pro Pro Phe Gly Asp Ser Tyr Ile Ile Ile 
65                  70                  75                  80  


Gly Val Glu Pro Gly Gln Leu Lys Leu Asn Trp Phe Lys 
                85                  90              


<210>  19
<211>  93
<212>  PRT
<213>  Dengue virus type 3

<400>  19

Met Cys Thr Asn Thr Phe Val Leu Lys Lys Glu Val Ser Glu Thr Gln 
1               5                   10                  15      


His Gly Thr Ile Leu Ile Lys Val Glu Tyr Lys Gly Glu Asp Ala Pro 
            20                  25                  30          


Cys Lys Ile Pro Phe Ser Thr Glu Asp Gly Gln Gly Lys Ala His Asn 
        35                  40                  45              


Gly Arg Leu Ile Thr Ala Asn Pro Val Val Thr Lys Lys Glu Glu Pro 
    50                  55                  60                  


Val Asn Ile Glu Ala Glu Pro Pro Phe Gly Glu Ser Asn Ile Val Ile 
65                  70                  75                  80  


Gly Ile Gly Asp Asn Ala Leu Lys Ile Asn Trp Tyr Lys 
                85                  90              


<210>  20
<211>  93
<212>  PRT
<213>  Dengue virus type 4

<400>  20

Met Cys Ser Gly Lys Phe Ser Ile Asp Lys Glu Met Ala Glu Thr Gln 
1               5                   10                  15      


His Gly Thr Thr Val Val Lys Val Lys Tyr Glu Gly Ala Gly Ala Pro 
            20                  25                  30          


Cys Lys Val Pro Ile Glu Ile Arg Asp Val Asn Lys Glu Lys Val Val 
        35                  40                  45              


Gly Arg Ile Ile Ser Ser Thr Pro Leu Ala Glu Asn Thr Asn Ser Val 
    50                  55                  60                  


Thr Asn Ile Glu Leu Glu Pro Pro Phe Gly Asp Ser Tyr Ile Val Ile 
65                  70                  75                  80  


Gly Val Gly Asn Ser Ala Leu Thr Leu His Trp Phe Arg 
                85                  90              


<210>  21
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HC of provided antibody agents

<400>  21

Glu Val Lys Leu Leu Glu Gln Ser Gly Ala Glu Leu Val Lys Pro Gly 
1               5                   10                  15      


Ala Ser Val Arg Leu Ser Cys Thr Ala Ser Gly Phe Asn Ile Lys Asp 
            20                  25                  30          


Thr Tyr Met Ser Trp Val Lys Gln Arg Pro Glu Gln Gly Leu Glu Trp 
        35                  40                  45              


Ile Gly Arg Ile Asp Pro Glu Asn Gly Asp Thr Lys Tyr Asp Pro Lys 
    50                  55                  60                  


Phe Gln Gly Lys Ala Thr Ile Thr Ala Asp Thr Ser Ser Asn Thr Ala 
65                  70                  75                  80  


Tyr Leu His Leu Ser Ser Leu Thr Ser Gly Asp Thr Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ser Arg Gly Trp Glu Gly Phe Ala Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ala 
        115         


<210>  22
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LC of provided antibody agents

<400>  22

Glu Leu Val Met Thr Gln Thr Pro Ala Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Asn Val Asp Lys Tyr 
            20                  25                  30          


Gly Asn Ser Phe Met His Trp Tyr Gln Gln Lys Ala Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Arg Ala Ser Glu Leu Gln Trp Gly Ile Pro Ala 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Arg Thr Asp Phe Thr Leu Thr Ile Asn 
65                  70                  75                  80  


Pro Val Glu Ala Asp Asp Val Ala Thr Tyr Phe Cys Gln Arg Ser Asn 
                85                  90                  95      


Glu Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
            100                 105                 110         


<210>  23
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HC CDR1 of provided antibody agents

<400>  23

Gly Phe Asn Ile Lys Asp Thr 
1               5           


<210>  24
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HC CDR2 of provided antibody agents

<400>  24

Asp Pro Glu Asn Gly Asp 
1               5       


<210>  25
<211>  7
<212>  PRT
<213>  artificial

<220>
<223>  HC CDR3 of provided antibody agents

<400>  25

Gly Trp Glu Gly Phe Ala Tyr 
1               5           


<210>  26
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LC CDR1 of provided antibody agents

<400>  26

Arg Ala Ser Glu Asn Val Asp Lys Tyr Gly Asn Ser Phe Met His 
1               5                   10                  15  


<210>  27
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LC CDR2 of provided antibody agents

<400>  27

Arg Ala Ser Glu Leu Gln Trp 
1               5           


<210>  28
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LC CDR3 of provided antibody agents

<400>  28

Gln Arg Ser Asn Glu Val Pro Trp Thr 
1               5                   


<210>  29
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized polypeptide

<400>  29

Glu Val Lys Leu Leu Glu Gln Ser Gly Ala Glu Leu Val Lys Pro Gly 
1               5                   10                  15      


Ala Ser Val Arg Leu Ser Cys Thr Ala Ser Gly Phe Asn Ile Lys Asp 
            20                  25                  30          


Thr Tyr Met Ser Trp Val Lys Gln Arg Pro Glu Gln Gly Leu Glu Trp 
        35                  40                  45              


Ile Gly Arg Ile Asp Pro Glu Asn Gly Asp Thr Lys Tyr Asp Pro Lys 
    50                  55                  60                  


Phe Gln Gly Lys Ala Thr Ile Thr Ala Asp Thr Ser Ser Asn Thr Ala 
65                  70                  75                  80  


Tyr Leu His Leu Ser Ser Leu Thr Ser Gly Asp Thr Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ser Arg Gly Trp Glu Gly Phe Ala Tyr Trp Gly Gln Gly Thr Leu 
            100                 105                 110         


Val Thr Val Ser Ala 
        115         


<210>  30
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized polypeptide

<400>  30

Glu Leu Val Met Thr Gln Thr Pro Ala Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Gln Arg Ala Thr Ile Ser Cys Arg Ala Ser Glu Asn Val Asp Lys Tyr 
            20                  25                  30          


Gly Asn Ser Phe Met His Trp Tyr Gln Gln Lys Ala Gly Gln Pro Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Arg Ala Ser Glu Leu Gln Trp Gly Ile Pro Ala 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Arg Thr Asp Phe Thr Leu Thr Ile Asn 
65                  70                  75                  80  


Pro Val Glu Ala Asp Asp Val Ala Thr Tyr Phe Cys Gln Arg Ser Asn 
                85                  90                  95      


Glu Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
            100                 105                 110         


<210>  31
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized polypeptide

<400>  31

Glu Val Lys Leu Leu Glu Gln Ser Gly Ala Glu Leu Val Lys Pro Gly 
1               5                   10                  15      


Ala Ser Val Arg Leu Ser Cys Thr Ala Ser 
            20                  25      


<210>  32
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized polypeptide

<400>  32

Tyr Met Ser Trp Val Lys Gln Arg Pro Glu Gln Gly Leu Glu Trp Ile 
1               5                   10                  15      


Gly Arg Ile 
            


<210>  33
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized polypeptide

<400>  33

Thr Lys Tyr Asp Pro Lys Phe Gln Gly Lys Ala Thr Ile Thr Ala Asp 
1               5                   10                  15      


Thr Ser Ser Asn Thr Ala Tyr Leu His Leu Ser Ser Leu Thr Ser Gly 
            20                  25                  30          


Asp Thr Ala Val Tyr Tyr Cys Ser Arg 
        35                  40      


<210>  34
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized polypeptide

<400>  34

Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala 
1               5                   10      


<210>  35
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized polypeptide

<400>  35

Gly Phe Asn Ile Lys Asp Thr 
1               5           


<210>  36
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized polypeptide

<400>  36

Asp Pro Glu Asn Gly Asp 
1               5       


<210>  37
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized polypeptide

<400>  37

Gly Trp Glu Gly Phe Ala Tyr 
1               5           


<210>  38
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized polypeptide

<400>  38

Glu Leu Val Met Thr Gln Thr Pro Ala Ser Leu Ala Val Ser Leu Gly 
1               5                   10                  15      


Gln Arg Ala Thr Ile Ser Cys 
            20              


<210>  39
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized polypeptide

<400>  39

Trp Tyr Gln Gln Lys Ala Gly Gln Pro Pro Lys Leu Leu Ile Tyr 
1               5                   10                  15  


<210>  40
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized polypeptide

<400>  40

Gly Ile Pro Ala Arg Phe Ser Gly Ser Gly Ser Arg Thr Asp Phe Thr 
1               5                   10                  15      


Leu Thr Ile Asn Pro Val Glu Ala Asp Asp Val Ala Thr Tyr Phe Cys 
            20                  25                  30          


<210>  41
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized polypeptide

<400>  41

Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
1               5                   10      


<210>  42
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized polypeptide

<400>  42

Arg Ala Ser Glu Asn Val Asp Lys Tyr Gly Asn Ser Phe Met His 
1               5                   10                  15  


<210>  43
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized polypeptide

<400>  43

Arg Ala Ser Glu Leu Gln Trp 
1               5           


<210>  44
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically synthesized polypeptide

<400>  44

Gln Arg Ser Asn Glu Val Pro Trp Thr 
1               5                   


