                         SEQUENCE LISTING

<110>  BASE4 INNOVATION LIMITED
 
<120>  METHYLATION DETECTION METHOD

<130>  P59960WO

<150>  GB 1402644.7
<151>  2014-02-14

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  113
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  single-stranded oligonucleotide precursor


<220>
<221>  modified_base
<222>  (28)..(28)
<223>  m5c

<220>
<221>  misc_feature
<222>  (101)..(102)
<223>  T bases labelled with Quasar 570

<220>
<221>  misc_feature
<222>  (103)..(103)
<223>  T base labelled with BHQ-2 quencher

<220>
<221>  misc_feature
<222>  (104)..(105)
<223>  T bases labelled with Quasar 570

<220>
<221>  misc_feature
<222>  (112)..(113)
<223>  phosphorothioate linkage

<400>  1
gtaggtcctg gaaacgaaaa acggaggcca gaagcaaaaa gcagggacgt gatgttccat       60

gactgatttt tttttcagtc atggaacatc acgtccctgc tttttgcttc tgg             113


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  single-stranded oligonucleotide precursor


<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  T bases labelled with Alexa Fluor 633

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  T base labelled with BHQ-2 quencher

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  T bases labelled with Alexa Fluor 633

<400>  2
ctccgttttt cgtttccaga                                                   20


<210>  3
<211>  113
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  single-stranded oligonucleotide precursor


<220>
<221>  misc_feature
<222>  (101)..(102)
<223>  T bases labelled with Quasar 570

<220>
<221>  misc_feature
<222>  (103)..(103)
<223>  T base labelled with BHQ-2 quencher

<220>
<221>  misc_feature
<222>  (104)..(105)
<223>  T bases labelled with Quasar 570

<220>
<221>  misc_feature
<222>  (112)..(113)
<223>  phosphorothioate linkage

<400>  3
gtaggtcctg gaaacgaaaa acggacgtac gaagcaaaaa gcagggacgt gatgttccat       60

gactgatttt tttttcagtc atggaacatc acgtccctgc tttttgcttc gta             113


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  single-stranded oligonucleotide precursor


<220>
<221>  misc_feature
<222>  (6)..(7)
<223>  T bases labelled with Alexa Fluor 633

<220>
<221>  misc_feature
<222>  (8)..(8)
<223>  T base labelled with BHQ-2 quencher

<220>
<221>  misc_feature
<222>  (9)..(10)
<223>  T bases labelled with Alexa Fluor 633

<400>  4
gtccgttttt cgtttccaga                                                   20


